| effect of biocontrol strains of trichoderma on plant growth, pythium ultimum polulations, soil microbial communities and soil enzyme activities. | five strains of trichoderma with known biocontrol activities were assessed for their effect upon pea growth and their antagonistic activity against large pythium ultimum inocula. the effect of trichoderma inocula upon the indigenous soil microflora and soil enzyme activities in the presence and absence of pythium is assessed. in the absence of pythium, trichoderma strain n47 significantly increased the wet shoot weight by 15% but did not significantly affect the dry weight, whilst strains t4 and ... | 2000 | 10735255 |
| aggressive multimodality therapy for stage iii esophageal cancer: a phase i/ii study. | stage iii advanced locoregional esophageal carcinoma is frequently unresectable and inconsistently represented in therapeutic trials of esophageal cancer. | 2000 | 10735664 |
| dopaminergic and cholinergic involvement in the inhibitory effect of dexamethasone on the tsh response to trh. | glucocorticoid administration is associated with reduced basal thyroid-stimulating hormone (tsh) levels and a blunted tsh response to thyrotropin-releasing hormone (trh), despite thyroid hormone levels within the normal range. in light of the inhibitory effect of somatostatin and dopamine on tsh secretion, we examined whether this condition is caused by glucocorticoids through an increased hypothalamic somatostatinergic and/or dopaminergic inhibitory control of tsh. we measured the tsh response ... | 2000 | 10736973 |
| prognostic indicators for renal cell carcinoma: a multivariate analysis of 643 patients using the revised 1997 tnm staging criteria. | we determine independent prognostic indicators for renal cell carcinoma using the revised 1997 tnm staging criteria. | 2000 | 10737472 |
| potent and selective tools to investigate neuropeptide y receptors in the central and peripheral nervous systems: bib03304 (y1) and cgp71683a (y5). | we have evaluated 3 newly developed neuropeptide y receptor antagonists in various in vitro binding and bioassays: bibo3304 (y1), t4[npy33-36]4 (y2), and cgp71683a (y5). in rat brain homogenates, bibo3304 competes for the same population of [125i][leu31,pro34] peptide yy (pyy) binding sites (75%) as bibp3226, but with a 10 fold greater affinity (ic50 of 0.2 +/- 0.04 nm for bibo3304 vs. 2.4 +/- 0.07 nm for bibp3226),while cgp71683a has high affinity for 25% of specific [125i][leu31,pro34]pyy bind ... | 2000 | 10737674 |
| [prognostic influence of cutaneous involvement in malignant tumors of the oral cavity]. | the aim of this study was to assess the prognostic influence of cutaneous involvement in t4 squamous cell carcinoma of the oral cavity. the population was a homogeneous group of 137 patients. cutaneous tumor localizations were observed in 20 of them. surgery and radiotherapy were given in 103 cases and salvage surgery was performed in 34. local control at 5 years was achieved in 68.5 % of the patients. carcinologic failure rate was 55 % and 27.3 % in patients with and without cutaneous involveme ... | 2000 | 10739997 |
| hyperbaric bupivacaine for spinal anaesthesia in 7-18 yr old children: comparison of bupivacaine 5 mg ml-1 in 0.9% and 8% glucose solutions. | we have compared two hyperbaric bupivacaine solutions for spinal anaesthesia in 7-18-yr-old school-aged children in a double-blind, randomized, parallel group, prospective study. children were premedicated with diazepam orally. half of the patients were sedated with either midazolam or thiopental. after lumbar puncture with a 27-gauge spinal needle, bupivacaine 5 mg ml-1 in either 0.9% or 8% glucose was injected in a dose of 0.3 mg kg-1. maximum cephalad spread and regression of block were teste ... | 2000 | 10740548 |
| [partial laryngectomy for treatment of the laryngeal cancer of t3 and t4 categories]. | between 1980 to 1993, 31 patients (27 men and 4 women) with squamous cell carcinoma of the larynx with t3,4 categories undergone partial largngectomy. the 3- and 5-year survival rates were 64.5% and 59.3% respectively. the extubation rate was 71.0% and recurrence rate 19.4%. after opration, the 31 patients have acquired speech function of different degrees and the swallow function returned to normal. the authors concluded that in selected patients with t3,4 laryngeal cancers, partial laryngectom ... | 1997 | 10743107 |
| [clinical analysis of bilateral neck dissection in patients with laryngeal cancer]. | the clinical materials of 54 cases with laryngeal cancer treated between 1980 and 1995 were retrospectively analysed. all patients had received bilateral neck dissection. the results indicated that if one internal jugular vein was preserved, bilateral neck dissection was safe and no fatal complications occur. in this series, most patients were of t3 and t4. the 3 and 5 year survival rates were 62% and 52% respectively. cervical recurrence was the main reason for the deaths. it is suggested that ... | 1997 | 10743129 |
| [on the mechanism of hemilaryngeal fixation in pyriform sinus carcinoma and its clinical significance]. | to approach the mechanism of hemilaryngeal fixation in pyriform sinus carcinoma (psc), surgical specimens from total laryngectomy and partial hypopharyngectomy in 43 cases of pyriform sinus carcinoma were studied using organ subserial sections. invasion of intralaryngeal muscles and the upper and outer aspects of arytenoid cartilage via paraglottic space was found to be the main cause of hemilaryngeal fixation in these cases. presence of tumor on the medial wall of the pyriform sinus makes invol ... | 1997 | 10743148 |
| [lysosomal acid lipase deficiency. overview of czech patients]. | lysosomal lipase deficiency is a hereditary autosomal recessive enzymopathy leading to lysosomal storage of triacylglycerols (tag) and cholesterol esters (ce). in particular cells with a permanently high receptor-mediated ldl endocytosis are affected (liver, kidneys). there are two basic phenotypes. the fatal infantile phenotype (wolman's disease) with generalized storage of both types of apolar lipids. this form was diagnosed in this country only once. the opposite is the protracted, oligosympt ... | 1999 | 10746035 |
| [new possibilities in the diagnosis and documentation using 3d power doppler ultrasound with carcinoma of the floor of the mouth as illustration]. | to evaluate a novel 3d power doppler ultrasound-technique (3d pdus) in the diagnosis and documentation of tumours of the floor of the mouth. | 2000 | 10746281 |
| long-term alterations in adiposity affect the expression of melanin-concentrating hormone and enkephalin but not proopiomelanocortin in the hypothalamus of ovariectomized ewes. | we have developed a ruminant model to study long-term alterations in adiposity on the expression of appetite-regulating peptides in the hypothalamus. in this model endocrine and metabolic status are fully defined as well as body composition. the current study sought to define the effects of altered adiposity on the expression of genes for neuropeptide y (npy), pomc, enkephalin (enk), and melanin-concentrating hormone (mch). ovariectomized ewes with high (60 +/- 1 kg) (fat) or low (37 +/- 3 kg) b ... | 2000 | 10746657 |
| hla class i antigens expression in renal cell carcinoma: histopathological and clinical correlation. | immunohistochemical analysis of hla class i antigens expression in 26 renal cell carcinomas (rccs)--18 clear cell, 6 granular and 2 chromophobe--was performed with indirect immunoperoxidase method. results were correlated with extent and immunophenotype of tumor infiltrating mononuclear cells, histopathological (histology, cytology, grade, presence of necrosis) and clinical (tumor diameter, tnm classification) characteristics of rcc. 4 (15%) rccs showed reduced hla class i presence and this was ... | 1999 | 10746977 |
| cysteines involved in radical generation and catalysis of class iii anaerobic ribonucleotide reductase. a protein engineering study of bacteriophage t4 nrdd. | class iii ribonucleotide reductase (rnr) is an anaerobic glycyl radical enzyme that catalyzes the reduction of ribonucleotides to deoxyribonucleotides. we have investigated the importance in the reaction mechanism of nine conserved cysteine residues in class iii rnr from bacteriophage t4. by using site-directed mutagenesis, we show that two of the cysteines, cys-79 and cys-290, are directly involved in the reaction mechanism. based on the positioning of these two residues in the active site regi ... | 2000 | 10748010 |
| allosteric regulation of the class iii anaerobic ribonucleotide reductase from bacteriophage t4. | ribonucleotide reductase (rnr) is an essential enzyme in all organisms. it provides precursors for dna synthesis by reducing all four ribonucleotides to deoxyribonucleotides. the overall activity and the substrate specificity of rnr are allosterically regulated by deoxyribonucleoside triphosphates and atp, thereby providing balanced dntp pools. we have characterized the allosteric regulation of the class iii rnr from bacteriophage t4. our results show that the t4 enzyme has a single type of allo ... | 2000 | 10748029 |
| determination of the free-energy change for repair of a dna phosphodiester bond. | the repair of phosphodiester bonds in nicked dna is catalyzed by dna ligases. ligation is coupled to cleavage of a phosphoanhydride bond in a nucleotide cofactor resulting in a thermodynamically favorable process. a free energy value for phosphodiester bond formation was calculated using the reversibility of the t4 dna ligase reaction. the relative number of dna nicks to phosphodiester bonds in a circular plasmid dna, formed during this reaction at fixed concentrations of atp to amp and pp(i), w ... | 2000 | 10748184 |
| results of surgical resection of oesophageal carcinoma in singapore. | the 5-year survival rate after resection of oesophageal cancer is still poor, reported to be between 5% to 34% in the literatures. we conducted this retrospective study to determine the prognostic factors and long-term survival rate for surgical resection of oesophageal carcinoma in singapore. | 2000 | 10748966 |
| radical radiotherapy for inoperable non-small cell lung cancer: what factors predict prognosis? | we set out to determine the factors that predict the outcome of conventional radical radiotherapy for inoperable non-small cell lung cancer. a retrospective casenote review was carried out of all 69 patients treated between 1986 and 1992 at the northern ireland centre for clinical oncology, belfast, with radical radiotherapy for inoperable non-small cell lung cancer. the tumour dose ranged from 45 gy to 67.5 gy, delivered in 15-30 fractions, 5 days per week over 3-6 weeks. all patients were foll ... | 2000 | 10749020 |
| diagnosis of hypothyroidism in a western lowland gorilla (gorilla gorilla gorilla) using human thyroid-stimulating hormone assay. | primary hypothyroidism was diagnosed in a 26-yr-old female western lowland gorilla (gorilla gorilla gorilla) on the basis of serum levels of thyroxine (t4), free t4, and thyroid-stimulating hormone (tsh) measured by human immunoassays. compared with clinically normal gorillas, the tsh level (107 mliu/l) was markedly elevated, and t4 (<14.0 nmol/l) and free t4 (5.0 pmol/l) levels were decreased. thyroid hypofunction could explain the weight gain, unsettled appetite, anxious behavior, lethargy, an ... | 1999 | 10749441 |
| the central melanocortin system affects the hypothalamo-pituitary thyroid axis and may mediate the effect of leptin. | prolonged fasting is associated with a downregulation of the hypothalamo-pituitary thyroid (h-p-t) axis, which is reversed by administration of leptin. the hypothalamic melanocortin system regulates energy balance and mediates a number of central effects of leptin. in this study, we show that hypothalamic melanocortins can stimulate the thyroid axis and that their antagonist, agouti-related peptide (agrp), can inhibit it. intracerebroventricular (icv) administration of agrp (83-132) decreased pl ... | 2000 | 10749579 |
| azu-1: a candidate breast tumor suppressor and biomarker for tumor progression. | to identify genes misregulated in the final stages of breast carcinogenesis, we performed differential display to compare the gene expression patterns of the human tumorigenic mammary epithelial cells, hmt-3522-t4-2, with those of their immediate premalignant progenitors, hmt-3522-s2. we identified a novel gene, called anti-zuai-1 (azu-1), that was abundantly expressed in non- and premalignant cells and tissues but was appreciably reduced in breast tumor cell types and in primary tumors. the azu ... | 2000 | 10749935 |
| morphometric x-ray absorptiometry and morphometric radiography of the spine: a comparison of prevalent vertebral deformity identification. | prevalent vertebral deformities are associated with a substantially increased risk of subsequent vertebral and nonvertebral fractures. knowledge of vertebral fracture status is an important component in the prediction of further fractures in patients with osteoporosis. this study reports a comparison of the quantitative identification of vertebral deformities on morphometric x-ray absorptiometry (mxa) scans and conventional radiographs (mrx) in 161 postmenopausal women (mean age +/- sd, 64 +/- 7 ... | 2000 | 10750572 |
| combined resection of t4 lung cancer with invasion of the descending thoracic aorta. | | 2000 | 10750808 |
| dual thyroid ectopy: case report and review of the literature. | dual ectopic thyroid glands rarely occur, and only six cases have been reported in the literature. the authors describe a 14-year-boy who had a midline neck swelling for 8 to 9 years. the swelling increased gradually, but he had no pressure symptoms. the thyroid hormone profile showed a moderately increased thyroid-stimulating hormone value and normal t3 and t4 levels. a tc-99m sodium pertechnetate thyroid scan showed dual ectopic thyroid glands in the sublingual and subhyoid regions. | 2000 | 10750961 |
| [study on the p53 gene mutation and microsatellite instability of gastric carcinoma]. | to observe the relationship between p53 gene mutation and microsatellite instability (msi) of the preoperative staging of gastric carcinoma by endoscopic ultrasonography(eus). | 2000 | 10751531 |
| vestibular evoked potentials (vestep) and brain electrical activity mapping - a test of vestibular function - a review (1990 - 1996). | brain electrical activity mapping of vestibular evoked potentials (beam-vestep) is a new technology for investigation of the spatial and temporal properties of a rotationally-induced brain electrical events. the method consists of multichannel eeg registration and mapping of the brain isoelectrical contours during short-lasting repetitive angular accelerations. a special data bank containing more than 400 beam-vestep investigations on more than 300 persons, either symptom free volunteers or neur ... | 1996 | 10753341 |
| cutting edge: activation of hiv-1 transcription by the mhc class ii transactivator. | both macrophages and activated cd4+ t cells can be productively infected by hiv-1, and both cell types express mhc class ii molecules. expression of mhc class ii proteins in these cells is regulated by a specific transcriptional coactivator, the class ii transactivator (ciita). in this study, we report for the first time that ciita expression profoundly influences hiv-1 replication. stable expression of ciita in jurkat cells markedly increased 1) hiv-1 replication as assessed by the p24 ag produ ... | 2000 | 10754282 |
| quantitative eeg in schizophrenic patients before and during pharmacotherapy. | the aim of the study was to determine the possible differences in quantitative eeg parameters of schizophrenic patients before and during therapy with neuroleptics. first eeg recordings were obtained from schizophrenic patients (n = 50) who had not been taking any medicaments during the preceding 2 months. second eeg recordings were obtained during the administration of neuroleptic therapy. amplitude values of particular spectral segment, i.e. delta, theta, alpha 1, alpha 2, beta 1 and beta 2 (a ... | 2000 | 10754432 |
| loss of heterozygosity analysis on chromosome 5p defines 5p13-12 as the critical region involved in tumor progression of bladder carcinomas. | we have previously observed loss of heterozygosity (loh) at a single locus (del-27) on human chromosome 5p13-12 to correlate with bladder tumor progression. in this study, we examined 33 bladder tumors for their pattern of allelic loss on chromosome 5p using 7 microsatellite markers. in 14 of 15 bladder tumors with loh at locus del-27, allelic loss was confined to chromosomal region 5p13-12. this region included the microsatellite marker d5s2025 that showed loh in 5 of 11 (45%) informative cases ... | 2000 | 10754499 |
| recombination-dependent dna replication in phage t4. | studies in the 1960s implied that bacteriophage t4 tightly couples dna replication to genetic recombination. this contradicted the prevailing wisdom of the time, which staunchly supported recombination as a simple cut-and-paste process. more-recent investigations have shown how recombination triggers dna synthesis and why the coupling of these two processes is important. results from t4 were instrumental in our understanding of many important replication and recombination proteins, including the ... | 2000 | 10754548 |
| the incidence and mechanisms of pharyngeal and upper esophageal dysfunction in partially paralyzed humans: pharyngeal videoradiography and simultaneous manometry after atracurium. | residual neuromuscular block caused by vecuronium alters pharyngeal function and impairs airway protection. the primary objectives of this investigation were to radiographically evaluate the swallowing act and to record the incidence of and the mechanism behind pharyngeal dysfunction during partial neuromuscular block. the secondary objective was to evaluate the effect of atracurium on pharyngeal function. | 2000 | 10754616 |
| effect of hypothyroidism on the testes in mature rats and treatment with levothyroxine and zinc. | this study was performed to investigate the effect of propylthiouracil (ptu) on rat testis, and to compare the results of the different treatment regimens of levothyroxine and zinc. twenty sexually mature wistar albino rats were subjected to ptu for 14 days intraperitoneally to make them hypothyroidic. the effect of ptu on testicular function was assessed histopathologically after unilateral orchiectomy on day 15, and treatment was evaluated by measuring serum thyroid-stimulating hormone (tsh), ... | 2000 | 10755190 |
| inhibition of gap-junctional-intercellular communication in thyroid-follicular cells by propylthiouracil and low iodine diet. | propylthiouracil (ptu) or low-iodine diet (lid) treatment increases thyroid-follicular-cell proliferation, possibly by disrupting the movement of small molecules (< 1.2 kd) through membrane channels called gap junctions. numerous tumor promoters and proliferative disease states exhibit inhibited gap-junctional-intercellular communication (gjic) prior to the induction of cell proliferation, yet the association between gjic and apoptosis is unclear. in the present study, we used an ex vivo method ... | 2000 | 10755705 |
| measurement of free thyroxine (t4) levels in pregnancy. | free t4 and tsh were measured in 119 pregnant women. twenty of these women had the free t4 measured a second time during the pregnancy; 35 of the 119 had the free t4 measured by 2 different methods. the results showed that free t4 fell as pregnancy progressed but even at the early stages of pregnancy many measured free t4 concentrations were below the lower end of the nonpregnant normal range. this remained the case when the measurement was made by a dialysis kit method. these results challenge ... | 1999 | 10755769 |
| type ii and type iii monodeiodinase activities in the skin of untreated and propylthiouracil-treated cashmere goats. | the presence or absence of types i, ii and iii iodothyronine monodeiodinase enzymes (mdi, mdii and mdiii) and their levels of activity in the skin of goats, which were orally dosed for 60 days with 0, 1.1, 2.2, 4.4, 8.8, 17.5, or 35 mg(-1)kg liveweight day(-1)of the anti-thyroid, enzyme-inhibiting drug, propylthiouracil (ptu), were determined. contrary to our earlier report that ptu did not influence skin mdii activity, the currect more thorough investigation (in terms of numbers of observations ... | 2000 | 10756128 |
| evidence of interactions between gp27 and gp28 constituents of the central part of bacteriophage t4 baseplate. | the central part of the bacteriophage t4 baseplate consists of several proteins. however, for a number of the constituents the manner of incorporation are not convincingly established. recently, we have presented evidence that gp28 is the structural component of the central part of the baseplate, which possesses a hydrophobic region and is membrane bound [nieradko et al., 1998]. by utilizing extracts prepared from escherichia coli cells that overexpressed genes 27 and 28 of phage t4, we proved t ... | 1999 | 10756710 |
| evidence for colorectal cancer micrometastases using reverse transcriptase-polymerase chain reaction analysis of muc2 in lymph nodes. | poor survival in patients following resection for early stage colorectal cancer is thought to be due in part to the presence of occult micrometastases at the time of surgery. the muc2 mucin gene is highly expressed in the colon and associated colorectal tumors and may be a candidate marker for colorectal cancer micrometastases. we have used rt-pcr to detect expression of muc2 mrna transcripts in order to identify possible lymph node micrometastases in node negative (stage i and ii, or dukes a an ... | 2000 | 10757125 |
| association of the antidiabetic effects of bromocriptine with a shift in the daily rhythm of monoamine metabolism within the suprachiasmatic nuclei of the syrian hamster. | bromocriptine, a dopamine d2 agonist, inhibits seasonal fattening and improves seasonal insulin resistance in syrian hamsters. alterations in daily rhythms of neuroendocrine activities are involved in the regulation of seasonal metabolic changes. changes in circadian neuroendocrine activities that regulate metabolism are believed to be modulated by central circadian oscillators within the hypothalamic suprachiasmatic nuclei (scn) of seasonal animals. we examined the association of metabolic resp ... | 2000 | 10757461 |
| comparison of hypotensive epidural anaesthesia and spinal anaesthesia on blood loss and coagulation during and after total hip arthroplasty. | hypotensive epidural anaesthesia (hea) is a technique for reducing peroperative blood loss by significantly lowering mean arterial pressure (map). | 2000 | 10757582 |
| the use of carboplatin and paclitaxel with daily radiotherapy in patients with locally advanced squamous cell carcinomas of the head and neck. | unresectable squamous cell carcinomas of the head and neck (scchn) continue to pose a significant therapeutic challenge. this report defines the toxicities, efficacy, and prognostic factors associated with the combination of carboplatin (cbdca), paclitaxel, and once-daily radiation for patients with locally advanced disease. additionally, the pharmacokinetics of paclitaxel were investigated. | 2000 | 10758304 |
| surgery for t4 breast carcinoma: implications for local control. | despite increasing public awareness and widespread availability of mammography, many patients will present with locally advanced breast cancers. the role of surgery remains controversial. between 1993 and 1998, 47 of 393 (11.9%) breast cancer patients presented with t4 (inflammatory or locally advanced) carcinoma. we reviewed multimodality management, clinical response to neoadjuvant therapy, perioperative course and complications, and local control. forty-six women and one man were diagnosed wi ... | 2000 | 10759194 |
| missing secretory granules, dilated endoplasmic reticulum, and nuclear dislocation in the thyroid gland of rdw rats with hereditary dwarfism. | previous studies on the rdw rat have suggested that its dwarfism is caused primarily by dysfunction of the thyroid gland. in this study, rat thyroid glands were analyzed endocrinologically and morphologically to clarify the primary cause of dwarfism in the rdw rat. the rdw rat showed lowered thyroid hormone (t4 and t3) levels but elevated tsh in serum. the rdw thyroid gland was almost proportional in size and it was not goiter in gross inspection. our histological investigation produced three re ... | 2000 | 10760744 |
| spinal subdural tuberculous abscess. | spinal subdural abscess is rare and only 48 cases have been described to date. in this report, we present an additional spinal subdural tuberculous abscess. | 2000 | 10762199 |
| supracricoid partial laryngectomy with cricohyoidoepiglottopexy and cricohyoidopexy for glottic and supraglottic carcinomas. | to review the patients operated in our department with supracricoid partial laryngectomy with either cricohyoidoepiglottopexy (chep) (59 cases) or cricohyoidopexy (chp) (10 cases) technique, for primary or recurrent glottosupraglottic squamous cell carcinoma and compare the technique with other surgical or conservative approaches for treatment of laryngeal carcinoma. | 2000 | 10764009 |
| multilevel thoracic pedicle aplasia causing congenital thoracic kyphosis: case report. | congenital thoracic kyphosis is a rare cause of treatable myelopathy. multilevel thoracic pedicle aplasia as a cause of this deformity has not been previously reported in the literature. we report a case and describe the surgical management and outcome. | 2000 | 10764276 |
| gonadotropin-releasing hormone stimulates thyroid activity in a freshwater murrel, channa gachua (ham.), and carps, catla catla (ham.) and cirrhinus mrigala (ham.). | injections of mammalian gnrh (mgnrh), salmon gnrh (sgnrh), and a homologous murrel, channa punctatus, gnrh (cgnrh) to a murrel, channa gachua, and a carp, catla catla, at a dose of 1 microg/250 g body wt significantly increased plasma thyroxine (t4) levels above control. piscine gnrhs (sgnrh and cgnrh) had significantly greater stimulatory effects compared with mgnrh. to observe whether this stimulatory effect by gnrhs is direct or indirect, thyroid follicles were isolated from hypobranchial mus ... | 2000 | 10764556 |
| endoribonuclease regb from bacteriophage t4 is necessary for the degradation of early but not middle or late mrnas. | the regb endoribonuclease from bacteriophage t4 cleaves early mrnas specifically in the middle of the sequence ggag. we show here that regb is required for the degradation of bulk t4 early mrna. in the absence of regb, the chemical half-life of early transcripts is increased nearly fourfold, whereas their functional half-life is increased twofold. regb also regulates the translation of several prereplicative genes. the synthesis of several early proteins is down-regulated, probably as a conseque ... | 2000 | 10764573 |
| treatment results of esophageal carcinoma of clinical t3, t4m0: historical comparison between neoadjuvant chemoradiotherapy followed by surgery or definitive radiotherapy and conventional surgery. | we investigated results for thoracic t3-t4 esophageal squamous cell carcinoma in two strategies. the protocol group (46 patients) consisted of neoadjuvant chemoradiotherapy (44 gy, cddp, 5-fu), followed by either definitive radiotherapy (total 70 gy) for responders or surgery for non-responders. the surgery group (26 patients) underwent esophagectomy with regional lymph node dissection. nineteen of them received postoperative radiotherapy. surgical candidates in both groups received intraoperati ... | 2000 | 10767370 |
| approaching the upper thoracic vertebrae without sternotomy or thoracotomy: a radiographic analysis with clinical application. | the distribution of the lowest vertebra tangential to the suprasternal notch and the lowest intervertebral disc visualized above the sternum was determined on magnetic resonance imaging (mri) studies. the method is illustrated in seven patients undergoing upper thoracic spinal reconstruction to define a surgical approach without sternotomy or thoracotomy. | 2000 | 10767801 |
| bacterial colonisation during gtr treatment. a longitudinal analysis. | the purpose of the present study was to evaluate the long-term biological and clinical effects of a controlled delivery system releasing tetracyclines during the healing of interproximal periodontal defects treated by the technique of guided tissue regeneration (gtr) using e-ptfe membranes. | 1999 | 10768009 |
| [lumbar radical nephrectomy. a retrospective analysis of 186 cases]. | 186 cases of nephrectomy for renal cell cancer are studied retrospectively. the overall 5-year survival rate was 77.2%. according to the 4th edition of the "tnm" classification system of uicc the survival rates of patients with t2-(n = 97), t3a (n = 58) and t3b-tumors (n = 25) were 80.9%, 79.3% and 65.6%, respectively. no patient with a stage t4 tumor (n = 6) survived longer than 20 months. the 5-year survival rate of patients with n1-lymph node metastases was 83.3% (n = 8); without lymph node m ... | 2000 | 10768226 |
| clinical significance of autoimmune thyroid disease in myasthenia gravis. | we investigated thyroid function and autoimmunity in 74 (61% females) consecutive patients with mg. 30 of these patients were tested twice: the time between the investigations ranging from 1 to 4 years. mg was diagnosed on the basis of typical clinical symptoms, a positive tensilone-test, and/or detectable acetylcholine antibodies and/or repetetive stimulation tests. eye involvement was present in 86%, concurrent thymomas in 56%. the following parameters were measured in the serum of these patie ... | 2000 | 10768835 |
| metallothionein expression in transplantable mouse mammary tumors. | the metal binding protein, metallothionein (mt) is involved in various physiological processes. in various human tumors, moreover, mt is reported to play an important role in carcinogenesis. | 2000 | 10769683 |
| cervical coupling during lateral head translations creates an s-configuration. | to determine cervical coupling during the posture of lateral head translation relative to a fixed thoracic cage. | 2000 | 10771122 |
| developing chicken oligodendrocytes express the type iv oligodendrocyte marker t4-o in situ, but not in vitro. | accumulating data suggest that the oligodendrocyte population includes morphological and biochemical subtypes. we recently reported that a polyclonal antiserum against an unknown antigen, the t4-o molecule, labels a subpopulation of chicken oligodendrocytes, obviously representing the type iv variety of del rio hortega. the present study examines the developmental expression of the t4-o molecule in situ and in vitro. the results show that t4-o immunoreactive cells first appear at e15 in the vent ... | 2000 | 10771152 |
| [results of a survey of 549 patients regarding long-term clinical outcome and quality of life four years after ptca]. | quality control becomes increasingly important in interventional cardiology. since in most health care systems, clinical treatment of patients who underwent percutaneous transluminal coronary angioplasty (ptca) is left to general practitioners, important information on the clinical long-term outcome is lost for the cardiologic centers. aim of this study was to evaluate the clinical status of these patients 4 years after treatment with a ptca at our institution. | 2000 | 10771558 |
| thyroid function tests and their interpretation. | for general screening of children who are in fairly good health and in whom cns or pituitary disease is not strongly suspected, the newer ths assays are very useful in assessing the action of both endogenous and exogenous thyroid hormone. those children in whom primary hypothyroidism is strongly suspected or who are already on thyroid hormone supplementation, the free t4 assay provides a useful adjunct to the tsh. if grave's disease or factious hyperthyroidism is suspected, the total t3 assay is ... | 1997 | 10771809 |
| evaluation of a tier i screening battery for detecting endocrine-active compounds (eacs) using the positive controls testosterone, coumestrol, progesterone, and ru486. | after previously examining 12 compounds with known endocrine activities, we have now evaluated 4 additional compounds in a tier i screening battery for detecting endocrine-active compounds (eacs): a weak estrogen receptor (er) agonist (coumestrol; coum), an androgen receptor (ar) agonist (testosterone; test), a progesterone receptor (pr) agonist (progesterone; prog), and a pr antagonist (mifepristone; ru486). the tier i battery incorporates 2 short-term in vivo tests (5-day ovariectomized female ... | 2000 | 10774816 |
| [coliphages inactivation using chitosan derivatives]. | the effect of chitosan fragments with different degrees of polymerization and the chemical derivatives of chitosan differing in the number of amino groups and total molecule charge on phages t2, t4, and t7 was studied. the interaction of chitosan with bacteriophage particles inactivated them to the extent dependent on the chemical properties of chitosan and its concentration. phage t2 was found to be most susceptible to inactivation by chitosan. the polycationic nature of chitosan plays an impor ... | 2000 | 10776628 |
| [circadian rhythms of hypophysis-thyroid hormones secretion in patients with bronchial asthma]. | to examine circadian rhythms of hypophysiothyroid system in patients with bronchial asthma and effects of exogenic glucocorticoids on thyroid function. | 2000 | 10776645 |
| accelerated hyperfractionation for bronchogenic cancer: radiation therapy oncology group 9205. | rtog 92-05 was a phase ii trial developed to evaluate the feasibility, toxicity, and acceptance of a three times daily accelerated hyperfractionation radiation therapy schedule delivering 110 cgy, three times daily, to 79.2 gy uncorrected tumor dose in 72 fractions, in 24 treatment days, in patients with bronchogenic cancer. the radiographically visible tumor received accelerated hyperfractionation and the other radiation volume received standard hyperfractionation. three times a day, a dose of ... | 2000 | 10776986 |
| a t4-phage deoxycytidylate deaminase mutant that no longer requires deoxycytidine 5'-triphosphate for activation. | a deoxycytidylate (dcmp) deaminase encoded in t4-bacteriophage dna that is induced on phage infection of escherichia coli was shown earlier (maley, g. f., duceman, b. w., wang, a. m., martinez, j. m., and maley, f. (1990) j. biol. chem. 265, 47-51) to be similar in size, properties, and amino acid composition to the t2-phage-induced deaminase. neither enzyme is active in the absence of dctp or its natural activator, 5-hydroxymethyl-dctp. however, on changing the arginine (arg) at residue 115 of ... | 2000 | 10777550 |
| [total thyroxine (t4), free thyroxine (ft4)]. | | 1999 | 10778068 |
| hormonal profiles and estrogen receptors in egyptian female breast cancer patients. | hormones are considered to be an important factor in the etiology of breast cancer. serum hormonal profiles of premenopausal and postmenopausal breast cancer patients as well as estrogen receptor (er) concentrations in breast cancer tissues were examined in an attempt to establish a possible association between hormones and breast cancer risk and to elucidate the biological features of the disease among egyptian female patients. | 2000 | 10778762 |
| the rat hepatic lectin-1 subunit of the asialoglycoprotein receptor is upregulated by thyrotropin and downregulated by neoplastic transformation of thyroid cells. | we have recently shown that the rat hepatic lectin (rhl)-1 subunit of the asialoglycoprotein receptor (asgpr) is expressed in the pc c13 differentiated thyroid cell line. to investigate in vivo the expression of rhl-1 and the ability of thyrotropin (tsh) to modulate its expression, reverse-transcriptase polymerase chain reaction (rt-pcr) and western blot assays have been performed on thyroid extracts from rats treated with thyroxine (t4) or propylthiouracil (ptu), each of which modulates tsh lev ... | 2000 | 10779134 |
| maturation of human hypothalamic-pituitary-thyroid function and control. | measurements of serum thyrotropin (tsh) and free thyroxine (t4) concentrations were conducted in infants, children, and adults to assess maturation of the hypothalamic-pituitary-thyroid (hpt) feedback control axis. serum free t4 and tsh concentration data were collated for cord blood of the midgestation fetus, for premature and term infants, and for peripheral blood from newborn infants, children, and adults. mean values were plotted on a nomogram developed to characterize the reference ranges o ... | 2000 | 10779137 |
| neuropsychological follow-up in early-treated congenital hypothyroidism: a problem-oriented approach. | screening programs for congenital hypothyroidism (ch) dramatically improved the neuropsychological prognosis in affected children. however, mild impairments in cognitive performances, poorer motor skills, defective language abilities, and learning problems have been reported in some studies of early-treated ch children. the occurrence of these defects makes neuropsychological follow-up mandatory. the aim of the present study was to identify those neuropsychological functions that are more freque ... | 2000 | 10779139 |
| a case of sarcoidosis and sarcoid granuloma, papillary carcinoma, and graves' disease in the thyroid gland. | sarcoidosis is a systemic chronic granulomatous disease of unknown etiology most commonly affecting young females. the disease was first described in the thyroid gland in 1938. our patient, a 27-year-old male with known sarcoidosis, was referred to the national university hospital for acute symptoms of thyrotoxicosis (weight loss of 6 kg, tremor, thyroid enlargement, and tachycardia). laboratory findings showed suppressed serum thyrotropin (tsh, <0.03 mu/l [0.5-4.20]), increased total thyroxine ... | 2000 | 10779143 |
| [quality of life after treatment of laryngeal carcinoma: surgery versus radiotherapy]. | radiotherapy and surgery for laryngeal cancer achieve comparable results in patient survival. therefore, the expected quality of life is increasingly influencing the choice of treatment. the aim of this study was to compare the quality of life of patients after surgery or radiotherapy for laryngeal carcinoma. to evaluate quality of life, we used the validated european organization for research and treatment of cancer quality of life questionnaire core 30 (eortc qlq-c30) and the eortc head and ne ... | 2000 | 10780067 |
| [hypothyroidism after combined surgical and radiotherapy treatment of cancer of the orl area]. | hypothyroidism is a well described complication of head and neck cancer treatment, but routine testing of thyroid function is generally not done. the combination of surgery and radiotherapy seems to increase the risk of thyroid hypofunction. | 2000 | 10780072 |
| nucleic acid synthesis for investigations of gene functions. | the chemical syntheses of genes encoding human c-ha-ras and t4 endonuclease v are described. these genes have been used for studies of mutagenesis by damaged bases and recognition of nucleic acids by proteins. the modes of interaction of photo-damaged dna and cognate antibodies have also been investigated by biochemical and physicochemical approaches. | 1999 | 10780357 |
| direct laser trapping of single globular dna molecules. | we show herein that a single t4 phage dna molecule which was transformed from coiled to globular state, was trapped directly by nd:yag laser (1064 nm). we also estimated trapping force of globular dna molecules. | 1999 | 10780398 |
| in vitro selection of deoxyribozymes with dna capping activity. | in vitro selection was used to isolate a series of deoxyribozymes from a pool of random-sequence dnas that catalyze an atp-dependent self-capping reaction. each deoxyribozyme catalyzes the transfer of the nucleoside and alpha-phosphate moieties of atp to the phosphate group located at its 5' terminus, thereby creating a 5',5'-pyrophosphate cap. this same pyrophosphate cap structure is formed by t4 dna ligase during the classical process of dna ligation. these dna capping enzymes representative o ... | 1999 | 10780467 |
| the effects of long-term caging and molt of single comb white leghorn hens on heterophil to lymphocyte ratios, corticosterone and thyroid hormones. | two commercial strains of 18-wk-old single comb white leghorn (scwl) hens, hyline w-36 and dekalb xl, were housed six hens per cage in layer cages at two densities (361 and 482 cm2 per bird) with two replications each per strain and density combination. the high density treatment contained 24 hens per replication, and the low density treatment contained 18 hens per replication. egg production was measured during the first egg production cycle, a molt (fast) period, and the first 4 wk of the seco ... | 2000 | 10780646 |
| quantitative eeg in 'positive' and 'negative' schizophrenia. | quantitative eeg has yielded different results in schizophrenia. the method of quantitative eeg has been relatively poorly used in the studies of the dichotomy into positive and negative schizophrenia. | 2000 | 10782551 |
| stability of bacteriophage t4 short tail fiber. | adsorption of t-even bacteriophages to the e. coli host cell is mediated by long and short tail fibers. bacteriophage t4 short tail fiber protein p12 was used to investigate the stability against thermal and chemical denaturation. purified p12 is thermostable with a melting point of 78 degrees c. guanidinium chloride-induced denaturation displayed strong hysteresis and an intermediate between 2 and 3 m denaturant. the transitions occur at 1.5 and 3.2 m denaturant as revealed by fluorescence spec ... | 2000 | 10782996 |
| aorto-coronary dissection during angioplasty in a patient with myxedema. | a 67-year-old man with overt hypothyroidism and medically controlled hypertension was admitted for coronary angiography because of exertional angina. his triiodothyronine (t3) and thyroxine (t4) levels had been low for 4 years. although signs and symptoms of hypothyroidism were apparent, his hypercholesterolemia was mild. coronary angiography revealed an eccentric stenosis in the distal portion of the right coronary artery and it was decided to perform angioplasty because his angina had continue ... | 2000 | 10783057 |
| conjugated linoleic acid effects on circulating hormones, metabolites and lipoproteins, and its proportion in fasting serum and erythrocyte membranes of swine. | conjugated linoleic acids (cla)# refer to a group of linoleic acid (18:2)-derived isomers with conjugated double bonds mostly at carbon atoms 9 and 11 or 10 and 12, and with all possible cis and trans combinations. cla is a newly recognized nutrient that functions to regulate energy retention and metabolism and that causes a serum lipoprotein profile considered to be less atherogenic. however, rodent models that have been frequently used for these studies are only of limited use because of disti ... | 1999 | 10784383 |
| pre- and postresection thoracic washings in non-small cell carcinoma of the lung: a cytological study of 44 patients without pleural effusion. | the presence of malignant pleural effusion in patients with non-small cell bronchogenic cancer has a poor prognostic significance and is indicative of advanced disease (t4, iiib). the present study will investigate the role of cytology and identify the various cellular components seen in thoracic washings, in the absence of an effusion, and will identify the potential pitfalls in diagnosing these specimens. the sensitivity, specificity, and positive and negative predictive values will be determi ... | 2000 | 10787140 |
| treating the patient with differentiated thyroid cancer with thyroglobulin-positive iodine-131 diagnostic scan-negative metastases: including comments on the role of serum thyroglobulin monitoring in tumor surveillance. | differentiated thyroid cancer (dtc) patients, especially the 10% to 15% at high risk of cancer-related death, should have long-term monitoring for detection of recurrence or metastasis. conventional radiologic and ultrasonographic imaging is useful for localization of recurrent or persistent disease. for patients who have had ablation of residual thyroid tissue, measurement of serum thyroglobulin (tg) levels and radioactive iodine (rai) imaging provide highly sensitive tools for early detection. ... | 2000 | 10787191 |
| increased killing of bacillus subtilis on the hair roots of transgenic t4 lysozyme-producing potatoes. | transgenic potato plants expressing the phage t4 lysozyme gene which are resistant to the plant-pathogenic enterobacterium erwinia carotovora subsp. carotovora have been constructed. the agricultural growth of these potatoes might have harmful effects on soil microbiota as a result of t4 lysozyme release into the rhizosphere. to assess the bactericidal effect of roots, we have developed a novel method to associate the cells of bacillus subtilis with hair roots of plants and to quantify the survi ... | 2000 | 10788351 |
| identification and typing of human papillomavirus (hpv) in squamous cell carcinoma of the oral cavity and oropharynx. | recent epidemiological research has raised the possibility of a connection between viral infection by the human papilloma virus (hpv) and the existence of squamous cell carcinoma of the oral cavity and oroharynx (scca). some 60 subtypes have been identified to date, and specific subtypes are associated with lesions or infections at specific tissue sites. twenty-three patients with scca of the oral cavity were studied for the presence of hpv in their tumours. hpv dna (all type 16) was isolated fr ... | 2000 | 10789410 |
| pancreatic or duodenal resection or both for advanced carcinoma of the right colon: is it justified? | the role of extended resections for locally advanced carcinomas of the right colon infiltrating duodenum and pancreas remains unclear. this investigation was undertaken to review our experience with pancreatic head or duodenal resections for advanced right-sided colon cancer. | 2000 | 10789739 |
| evaluation of the liaison thyroid chemiluminescence immunoassays. | the liaison thyroid hormone assays tsh, ft4, ft3, t4 and t3 were evaluated by determining the imprecision, the reference ranges, the functional sensitivity (tsh), the dilution characteristics (accuracy) (ft4, ft3), and the recovery after spiking (tsh, t4, t3). furthermore, inter-method comparisons were performed with following methods: elecsys (roche diagnostics; tsh), axsym (abbott diagnostics; tsh, ft4, ft3, t4), acs:180 (bayer diagnostics; all analytes), amerlex-m (johnson & johnson; t4) and ... | 2000 | 10791127 |
| postoperative results of left upper abdominal evisceration for advanced gastric cancer. | in spite of the improvement of surgical techniques, the prognosis of patients with advanced gastric cancer still remains poor. with the aim of achieving en bloc resection of primary tumor, peritoneal dissemination on the greater omentum and lymph node metastasis, left upper abdominal evisceration (luae) was performed for 75 patients. in this report, we investigated the prognostic difference between the luae group and standard gastrectomy (total gastrectomy + pancreatosplenectomy). | 2000 | 10791241 |
| comparison of radiotherapy alone with radiotherapy plus hyperthermia in locally advanced pelvic tumours: a prospective, randomised, multicentre trial. dutch deep hyperthermia group. | local-control rates after radiotherapy for locally advanced tumours of the bladder, cervix, and rectum are disappointing. we investigated the effect of adding hyperthermia to standard radiotherapy. | 2000 | 10791373 |
| oligomerization, secretion, and biological function of an anchor-free parainfluenza virus type 2 (pi2) fusion protein. | a number of studies indicate that the transmembrane domain, the cytoplasmic domain, or both regions of viral surface glycoproteins are involved in quaternary structure formation. in this report, the transmembrane domain and cytoplasmic tail coding sequence of the fusion (f) glycoprotein gene from parainfluenza type 2 virus was truncated by pcr and the resulting gene (pi2f') was expressed in hela-t4 cells by using the vaccinia virus-t7 transient expression system. pulse-chase experiments indicate ... | 2000 | 10792996 |
| temperature sensitive mutants of escherichia coli for trna synthesis. | an efficient method was devised to isolate temperature sensitive mutants of e. coli defective in trna biosynthesis. mutants were selected for their inability to express suppressor activity after su3(+)-transducing phage infection. in virtually all the mutants tested, temperature sensitive synthesis of trna(tyr) was demonstrated. electrophoretic fractionation of (32)p labeled rna synthesized at high temperature showed in some mutants changes in mobility of the main trna band and the appearance of ... | 1974 | 10793671 |
| nucleotide sequence determination of bacteriophage t4 glycine transfer ribonucleic acid. | the nucleotide sequence of a t4 trna with an anticodon for glycine has been determined using (32)p-labeled material from t4-infected cultures of escherichiacoli. the sequence is: pgcggauaucguauaaugmgdauuaccucagacuuccaapsicugaugaugugagtpsicgauucucauuauccgcucca-oh. the 74 nucleotide sequence can be arranged in the classic cloverleaf pattern for trnas. the anticodon of t4 trna(gly) is ucc with a possible modification of the u. the trna molecule would thus be expected to recognize the glycine codons ... | 1974 | 10793690 |
| the nucleotide sequence and genome organization of japanese iris necrotic ring virus, a new species in the genus carmovirus. | the genome of japanese iris necrotic ring virus (jinrv) consists of a positive-sense ssrna of 4014 nucleotides with six major open reading frames (orfs). a 5'-non-coding region of 31 nucleotides precedes the first initiation codon. like carnation mottle virus (carmv), the 5'-proximal three orfs encode a 26 kda protein (p26) and two readthrough proteins, i.e. an 85 kda putative rna replicase (p85) and a 99 kda protein (p99). the central orf encodes a small 8 kda protein (p8). the 3'-proximal orf ... | 2000 | 10795530 |
| cyclin d1 expression is useful as a prognostic indicator for advanced esophageal carcinomas, but not for superficial tumors. | the purpose of the present study was to define the overexpression of cyclin d1 in superficial and advanced esophageal carcinomas and to investigate whether the expression of this molecule indicates a poor prognosis. this study included 41 patients with superficial esophageal carcinomas (tis and t1) and 48 patients with advanced esophageal carcinomas (t2, t3, and t4). the expression of cyclin d1 in surgically resected specimens was evaluated immunohistochemically with a monoclonal antibody. posit ... | 2000 | 10795746 |
| systemic circulation and cerebral oxygenation during head-up tilt in spinal cord injured individuals. | to compare tilt-induced alterations in cardiovascular homeostasis and cerebral oxygenation of spinal cord-injured (sci) to able-bodied (ab) individuals. | 2000 | 10795936 |
| interactions of proteins in aqueous electrolyte solutions from fluorescence anisotropy and circular-dichroism measurements. | understanding aqueous protein-protein interactions is crucial for the development of a molecular-thermodynamic model for salt-induced protein precipitation. in addition, protein interactions are important in many disease states, including cataract formation and alpha-amyloid diseases. fluorescence anisotropy provides a means to measure intermolecular interactions. in this work, monomer-dimer equilibrium of the peptide t4 lys(11-36) was studied by fluorescence anisotropy over the ph range 4-7 and ... | 2000 | 10796032 |
| intracellular ph regulation in a nonmalignant and a derived malignant human breast cell line. | tumor cells in vivo often exist in an ischemic microenvironment that would compromise the growth of normal cells. to minimize intracellular acidification under these conditions, these cells are thought to upregulate h(+) transport mechanisms and/or slow the rate at which metabolic processes generate intracellular protons. proton extrusion has been compared under identical conditions in two closely related human breast cell lines: nonmalignant but immortalized hmt-3522/s1 and malignant hmt-3522/t ... | 2000 | 10797312 |
| [risk factors for lymphedema of the arm after mastectomy for breast cancer]. | postmastectomy lymphedema of the arm is frequently associated to different factors including axillary node involvement and local and regional treatment of breast cancer. our aims was to identify risk factors of postmastectomy lymphedema. from a retrospective analysis of 735 breast cancers treated in our institute, we found 61 lymphedema of the arm. we then describe our study population and identify by univariate et multivariate analysis the factors significantly associated to the disease. the ma ... | 1999 | 10797983 |
| spectroscopic studies on ethidium bromide binding to intramolecular parallel and antiparallel triple helices containing t*a:t and g*g:c triplets. | the interaction of ethidium bromide (eb), a dna intercalator, with two intramolecular triplexes 5'd(g4a4g4-[t4]-c4t4c4-[t4]-g4t4g4), 5'd(g4t4g4-[t4]-g4a4g4-[t4]-c4t4c4) ([t4] represents a stretch of 4 thymine residues) and their precursor duplexes has been investigated by circular dichroism, fluorescence and uv absorption spectroscopy. binding of eb induces a circular dichroism band in the region around 310 nm which is positive for the duplex forms but negative for the triplex forms. we observed ... | 2000 | 10798532 |
| haemodynamic changes during minimally invasive coronary artery bypass surgery using high-dose esmolol. | we aimed to investigate the effects of high-dose esmolol on haemodynamics and oxygen extraction in minimally invasive direct coronary artery bypass (midcab) surgery patients. | 2000 | 10799829 |
| high prevalence of thyroid abnormalities in a chilean psychiatric outpatient population. | the aim of the present study was to establish the prevalence of thyroid disturbances in patients consulting for panic and mood disorders. these data may be relevant because thyroid functional alterations affect the success of treatment in these pathologies. we studied prospectively 268 psychiatric outpatients (204 females and 64 males) diagnosed by dsm-iv criteria. we excluded patients with addictive disorders and major medical disease. we measured tsh, free t4 (ft4) and antimicrosomal antibodie ... | 2000 | 10800763 |
| identification of initiation sites for t4 lysozyme folding using cd and nmr spectroscopy of peptide fragments. | using cd and 2d (1)h nmr spectroscopy, we have identified potential initiation sites for the folding of t4 lysozyme by examining the conformational preferences of peptide fragments corresponding to regions of secondary structure. cd spectropolarimetry showed most peptides were unstructured in water, but adopted partial helical conformations in tfe and sds solution. this was also consistent with the (1)h nmr data which showed that the peptides were predominantly disordered in water, although in s ... | 2000 | 10801343 |