| t4 phage beta-glucosyltransferase: substrate binding and proposed catalytic mechanism. | beta-glucosyltransferase (bgt) is a dna-modifying enzyme encoded by bacteriophage t4 which catalyses the transfer of glucose (glc) from uridine diphosphoglucose (udp-glc) to 5-hydroxymethylcytosine (5-hmc) in double-stranded dna. the glucosylation of t4 phage dna is part of a phage dna protection system aimed at host nucleases. we previously reported the first three-dimensional structure of bgt determined from crystals grown in ammonium sulphate containing udp-glc. in this previous structure, we ... | 1999 | 10497034 |
| parameters of thyroid function in the endemic goitre of akungba and oke-agbe villages of akoko area of southwestern nigeria. | the parameters of thyroid function were determined in akungba and oke-agbe communities of akoko area, where goiter is endemic and compared to a control population of erinmo and ifewara in ijesha area, both areas being in southwestern nigeria. the two study areas have similar physical and geological settings. however, while the main carbohydrate source of the control population are yam and/or bread-fruit, the akoko population derives her carbohydrates mainly from cassava which could lead to incre ... | 1998 | 10497657 |
| cyclin d1, glutathione s-transferase, and cytochrome p450 genotypes and outcome in patients with upper aerodigestive tract cancers: assessment of the importance of individual genes using multivariate analysis. | gst, cyp, and ccnd1 genotypes have been associated with outcome in several cancers. accordingly, we have examined, in patients with one squamous cell carcinoma (scc) of the head and neck, associations between gstm1, gstt1, gstm3, gstp1, cyp2d6, cyp1a1, cyp2e1, and ccnd1 genotypes and the outcome parameters, tumor extension, histological grade, and presence of nodes. we used logistic regression to study, first, each gene individually and, second, in a step-wise model that included all of the gene ... | 1999 | 10498401 |
| prognostic value of le(y) and h antigens in oral tongue carcinomas. | to investigate the prognostic significance of the carbohydrate epitopes h and le(y) and their relationship with proliferation and apoptosis. | 1999 | 10499058 |
| bis[platinum(ii)] and bis[platinum(iv)] complexes with optically active bis(vicinal-1,2-diamines) and their interaction with dna. | bis[platinum(ii)] [cl2pt(ll)ptcl2] complexes 2,5 and 8 with chiral non-racemic ligands: 1a-c (ll = (r,r), (s,s) and (r,s) n,n'-bis(3,4-diaminobutyl)hexanediamide); 4a,b (ll = (r,r) and (s,s) n,n'-bis[3,4-bis(diaminobutyl)] urea); 7a-d (ll' = (r,r), (s,s), (r,s) and (s,r) 4,5-diamino-n-(3,4-diaminobutyl) pentanamide) and bis[platinum(iv)] complex 10-13 with ligands 1a,b and 4a,b have been prepared and characterized by ir, 1h, 13c and 195pt nmr spectra. the interactions of 2a-c, 5a, 5b, 8a-d and 1 ... | 1999 | 10499290 |
| iodine speciation in biological samples by capillary electrophoresis-inductively coupled plasma mass spectrometry. | a hyphenation of capillary electrophoresis (ce) to inductively coupled plasma mass spectrometry (icp-ms) was employed for the speciation of iodine. the separation method used a buffer sandwich of phosphate (ph 2.3), naoh, sodium dodecyl sulfate (sds) and borate buffer (ph 8.3) for stacking, aiming at sufficient separation of iodide, iodate, thyroxine (t4) and triiodothyronine (t3). these four iodine species were separated within 15 min and subsequently detected during a pressure-driven detection ... | 1999 | 10499349 |
| prognostic significance of fas and fas ligand expressions in human esophageal cancer. | esophageal carcinomas have recently been shown to express fas ligand (fasl) and down-regulate fas to escape from host immune surveillance. however, the prognostic importance of fas/fasl and their correlation with clinicopathological characteristics are yet to be delineated in this highly malignant carcinoma. specimens from 106 esophageal squamous cell carcinoma patients were used for immunohistochemical evaluation of fas, fasl, and cd8 expressions. fifty-two (49%) and 34 (32%) patients were posi ... | 1999 | 10499620 |
| predicting tumor failure in prostate carcinoma after definitive radiation therapy: limitations of models based on prostate-specific antigen, clinical stage, and gleason score. | in this report, we use new patient data to test three popular models developed to predict the outcome of definitive radiation therapy. the data come from 240 men with localized prostate cancer and who were treated with definitive radiation therapy at a community hospital. all three models tested were based on the three commonly available variables of pretreatment prostate-specific antigen (psa), gleason score, and tumor stage, and we used the cox proportional hazards model and the logistic regre ... | 1999 | 10499622 |
| efficacy of simulated intravenous test dose in the elderly during general anesthesia. | reliability of detecting unintentional intravascular injection of the epinephrine-containing test dose is improved by decreasing the heart rate (hr) threshold to 10 beats/min during combined epidural and general anesthesia. we have tested whether this modified hr criterion is still applicable in the anesthetized elderly patients. | 1999 | 10499748 |
| hiv-1 long terminal repeat modulation by glucocorticoids in monocytic and lymphocytic cell lines. | glucocorticoid gene regulation can be carried out through direct binding of glucocorticoid receptor to glucocorticoid responsive elements (gre), regulating directly gene transcription and modulating some signaling pathways. the human immunodeficiency virus type 1 (hiv-1) expression can be activated by different immunomodulators through binding of particular nuclear factors to its long terminal repeat (ltr). in order to investigate the effect of glucocorticoids in pathways that activate hiv-1 exp ... | 1999 | 10500286 |
| splanchnic perfusion during controlled hypotension with haemodilution under isoflurane anaesthesia in elderly patients. | we investigated the effects of controlled hypotension with haemodilution under isoflurane anaesthesia on splanchnic perfusion in elderly patients. we determined the intramucosal ph using gastric tonometry in 28 patients scheduled for hip surgery. patients without cardiac disease were assigned to two groups according to age. group a (adult patients, n = 14) included patients aged less than 60 years (range 29-58 years, 47 +/- 11 years, mean +/- sd) and group b (elderly patients, n = 14) more than ... | 1999 | 10500940 |
| measurement of vertebral body heights: ex vivo comparisons between morphometric x-ray absorptiometry, morphometric radiography and direct measurements. | morphometric x-ray absorptiometry (mxa) offers some potential advantages over spinal radiography for the quantitative evaluation of vertebral fractures in individuals with spinal osteoporosis. this ex vivo study examined the accuracy of mxa and quantitative morphometry (qm) for the evaluation of vertebral height, in relation to direct measurements from cadaveric vertebral columns. spinal radiographs and mxa scans were obtained from nine cadaveric vertebral columns (mean age at death 64 years). a ... | 1999 | 10501773 |
| identification of vertebral deformities in men: comparison of morphometric radiography and morphometric x-ray absorptiometry. | osteoporotic vertebral deformities may be detected by morphometric radiography (mr) using spinal radiographs, and by morphometric x-ray absorptiometry (mxa) using dual-energy x-ray absorptiometry. reference values for mr may not be appropriate for mxa, and reference values may be affected by gender and age. the aims of this study were to (1) compare mean deformity of vertebral height ratios for mr and mxa in men, (2) compare mean deformity for mxa in men and women, (3) compare mean wedge angle m ... | 1999 | 10501798 |
| serum fructosamine concentrations in hyperthyroid cats. | serum fructosamine concentrations were measured in 35 healthy cats and in 30 hyperthyroid cats before and 30 days after curative radioiodine ((131)i) treatment. hyperthyroid cats were divided into those with 30 day post-treatment total thyroxine (t4) concentrations within (eut4) or below (hypot4) the reference range. the median (semi-interquartile range, sir) fructosamine concentration was significantly lower in hyperthyroid compared with healthy cats (295. 0 (18.5) micromol l(-1)) both before ( ... | 1999 | 10502488 |
| [the laboratory diagnosis of autoimmune thyroid diseases in those who worked in the cleanup of the aftermath of the accident at the chernobyl atomic electric power station]. | serum antibodies to thyroid tissue antigens were tested by enzyme immunoassay and the thyroid hormonal status was analyzed in 80 liquidators of the chernobyl accident consequences. antibodies to thyroglobulin in concentration of 160.7 +/- 10.5 iu/ml were detected in 11 (13.7%) subjects 11-12 years after the accident. antibodies to thyroperoxidase were detected in this cohort: the reaction was positive in 2.5% cases and weakly positive in 5%. groups of liquidators with changed thyroid functional ... | 1999 | 10502927 |
| gugulu (commiphora mukul) induces triiodothyronine production: possible involvement of lipid peroxidation. | an investigation was made to find out the importance of gugulu (commiphora mukul) in thyroid function of mice and to reveal the possible involvement of lipid peroxidation (lpo), if any. while no marked change in the concentrations of serum thyroxine (t4) was observed, triiodoth yronine (t3) concentration and t3/t4 ratio were enhanced following the administration of gugulu extract (0.2 g/kg b. wt./d for 15 days). a concomitant decrease in lpo was also noticed in liver, the principal site of t3 ge ... | 1999 | 10503949 |
| clinical characteristics of amiodarone-induced thyrotoxicosis and hypothyroidism in japan. | since amiodarone was introduced in japan in 1992, the incidence of the drug-induced thyroid dysfunction has been increasing. we studied the thyroid function of 13 patients with amiodarone-induced thyrotoxicosis (ait) and 11 patients with amiodarone-associated hypothyroidism (aah) who had been referred to our institute in the last 6 years. ait and aah developed after 39+/-21 and 20+/-16 months of amiodarone treatment, respectively. one patient developed aah followed by ait. the ait ranged from su ... | 1999 | 10503998 |
| productive, physiological and biochemical changes in imported and locally born friesian and holstein lactating cows under hot summer conditions of egypt. | twenty each of healthy lactating friesian and holstein cows were studied. they were of similar age and body weight, nonpregnant in their third lactation and 80-100 days post partum. the investigation was carried out for 8 weeks on two farms at the same time during the hot summer season in egypt. the first farm was in demietta (north east of the nile delta, 31 degrees 40' n) on 20 friesian cows and the second was in fakous (east of the nile delta, 30 degrees 40' n) on 20 holstein cows. on each of ... | 1999 | 10504103 |
| folding of coliphage t4 short tail fiber in vitro. analysing the role of a bacteriophage-encoded chaperone. | the morphogenesis of the escherichia coli bacteriophage t4 depends on the presence of helper proteins which are not components of the mature virion. two bacteriophage-encoded proteins, p57 and p38, are required for the assembly of the bacteriophage t4 tail fibers. in the absence of p57, two polypeptides of the long fiber (p34 and p37) and that of the short tail fiber (p12) fail to trimerize. instead they form water-insoluble aggregates. co-expression of the genes 12 and 57 in vivo caused the for ... | 1999 | 10504409 |
| bacteriophage t4 resistance to lysis-inhibition collapse. | lysis inhibition is a mechanism of latent-period extension and burst-size increase that is induced by the t4 bacteriophage adsorption of t4-infected cells. mutants of t4 genes imm, sp and 5 (specifically the ts1 mutant of 5) display some lysis inhibition. however, these mutants experience lysis-inhibition collapse, the lysis of lysis-inhibited cells, earlier than wild-type-infected cells (i.e. their collapse occurs prematurely). lysis from without is a lysis induced by excessive t4 adsorption. g ... | 1999 | 10505404 |
| selective v2-receptor vasopressin antagonism decreases urinary aquaporin-2 excretion in patients with chronic heart failure. | aquaporin-2 (aqp-2), a water channel located on the apical membrane of collecting duct cells, regulates water reabsorption under the control of vasopressin (avp). using an antibody directed to human aqp-2, a quantitative western blot analysis was performed to determine the collecting duct responsiveness to an oral, nonpeptide, v2 receptor antagonist (vpa-985) in patients with chronic nyha ii and iii heart failure. standards were derived by conjugating the immunizing peptide to maleimide-activate ... | 1999 | 10505693 |
| [accuracy and dose dependency of the train-of-four count]. | the estimation of a nondepolarizing neuromuscular block using the train-of-four (tof) count shows wide differences compared to the mechanomyographic measurement. the purpose of this study was to evaluate the clinical significance of these differences. | 1999 | 10506315 |
| [thoracoscopy-guided management of the "anterior column". methods and results]. | anterior thoracoscopic interbody stabilization and fusion was performed in 163 patients. lesions treated were located between t4 and l3, most frequently occurring at t12/ l2. operative time decreased dramatically as experience was gained with the procedure. 2 patients early in the series successfully were converted to an open procedure. one positioning related pressure harm on the thoracodorsal nerve and one irritation of the l1 root at the entrance site were both transitory. postoperative contr ... | 1999 | 10506375 |
| pain and reduced mobility in complex regional pain syndrome i: outcome of a prospective randomised controlled clinical trial of adjuvant physical therapy versus occupational therapy. | there are no adequate comparative studies on physical therapy (pt) versus occupational therapy (ot) in patients with complex regional pain syndrome i (crps i). therefore, we conducted a prospective randomised clinical trial to assess their effectiveness. the outcomes regarding reducing pain and normalising active range of motion (arom) are discussed. included in the study were 135 patients who had been suffering from crps i of one upper extremity for less than one year. they were randomly assign ... | 1999 | 10506674 |
| frequency of noncontiguous lymph node involvement in patients with resectable nonsmall cell lung carcinoma. | this study was undertaken to investigate the patterns of lymph node spread and the frequency of involvement of noncontiguous lymph node stations in patients with nonsmall cell lung carcinoma who had complete surgical resection. | 1999 | 10506699 |
| a phase ii trial of methotrexate, cisplatin, 5-fluorouracil, and leucovorin in the treatment of invasive and metastatic urothelial carcinoma. | the current treatment of advanced urothelial carcinoma generates high response rates but is associated with poor overall survival. the current study evaluated the efficacy and toxicity of a new combination of active drugs in the treatment of urothelial carcinoma. | 1999 | 10506721 |
| histology and aspiration cytology of benign thyroid diseases. | the thyroid is an endocrine gland involved in the homeostatic regulation of the organism by means of its products, the hormones thyroxine (t4) and triiodothyronine (t3). its basic morphologic unit is the follicle, composed of thyrocytes producing the inactive hormonal form thyroglobulin which is stored in the center of the follicle. the diseases affecting the thyroid are classified into benign and malignant. benign diseases include goiter, either diffuse (graves' disease when hyperfunctioning) o ... | 1999 | 10509125 |
| thyrotoxicosis: clinical and laboratory assessment. | thyroid hormones are triiodothyronine (t3) and thyroxine (t4). the hypophysial thyrotropic hormone, thyroid stimulating hormone (tsh) is their physiologic regulator. thyrotoxicosis is characterized by clinical symptoms caused by high thyroid hormone concentrations. the commonest forms are: 1) toxic diffuse goiter (basedow-flajani-graves disease), 2) toxic multinodular goiter, 3) toxic adenoma. other less frequent forms are the iodide-induced, that during hashimoto thyroiditis, that from inapprop ... | 1999 | 10509130 |
| dose- and age-dependent alterations in choline acetyltransferase (chat) activity, learning and memory, and thyroid hormones in 15- and 30-day old rats exposed to 1.25 or 12.5 ppm polychlorinated biphenyl (pcb) beginning at conception. | 1. polychlorinated biphenyls (pcb) were released into the environment through improper disposal for decades, causing widespread contamination. slow biodegradation and lipophilic properties of pcb caused its persistence and concentration through food webs. exposure to these environmental contaminants through maternal transfer during early development has been associated with neurological and endocrinological alterations in several different organisms. 2. the present study extended a preliminary i ... | 1999 | 10509384 |
| severe symptoms following a massive intentional l-thyroxine ingestion. | l-thyroxine (t4) is commonly prescribed medication for hypothyroidism in humans and animals. overdose has generally resulted in limited symptomatology managed with sedatives and beta-adrenergic receptor antagonists. we describe the largest acute t4 ingestion ever reported, which resulted in a profound thyrotoxicosis, resistant to treatment. a 34-y-old man ingested 900 (0.8 mg) tablets of veterinary t4 (720 mg) and was given 60 g of activated charcoal. he became lethargic on post-ingestion days 2 ... | 1999 | 10509439 |
| viral encephalopathy and retinopathy of farmed marine fish species in italy. | viral encephalopathy and retinopathy, otherwise known as fish encephalitis or viral nervous necrosis (vnn), is an emerging problem in several farmed marine fish species in various geographic areas all over the world. since summer 1995, heavy losses affecting mainly juvenile and adult sea bass (dicentrarchus labrax) have been observed in several on-growing facilities in italy. dying fish show abnormal swimming behaviour and, at temperatures higher than 20-22 degrees c, mortality rates range betwe ... | 1999 | 10509726 |
| correlation between maternal thyroid function tests and endothelin in preeclampsia-eclampsia. | to investigate the relationship between results of maternal thyroid function tests and endothelin levels in preeclamptic or eclamptic women. | 1999 | 10511357 |
| [nucleic acid syntheses and molecular recognition]. | syntheses of nucleic acids are of importance not only in the natural product chemistry but also in the molecular recognition study in biology. this review describes syntheses of rna and dna, which were found to be related each other. deoxyinosine probes were developed for cloning genes with degenerated codons. synthetic genes for c-ha-ras and t4 endonuclease v provided new approaches in recognition of nucleic acids by proteins. antibodies specific for photo-damaged pyrimidines in dna have been s ... | 1999 | 10511816 |
| insulin resistance during puberty: results from clamp studies in 357 children. | insulin resistance may be an important cause of a constellation of cardiovascular risk factors in adults, and onset of this syndrome may occur in childhood. however, children normally experience transient insulin resistance at puberty. there were 357 normal children (159 girls, 198 boys) age 10-14 years who underwent euglycemic clamp studies to assess the effects of tanner stage (t), sex, ethnicity, and bmi on insulin resistance. insulin resistance increased immediately at the onset of puberty ( ... | 1999 | 10512371 |
| structural analysis of a non-contiguous second-site revertant in t4 lysozyme shows that increasing the rigidity of a protein can enhance its stability. | the mutation glu108-->val (e108v) in t4 lysozyme was previously isolated as a second-site revertant that specifically compensated for the loss of function associated with the destabilizing substitution leu99-->gly (l99g). surprisingly, the two sites are 11 a apart, with leu99 in the core and glu108 on the surface of the protein. in order to better understand this result we have carried out a detailed thermodynamic, enzymatic and structural analysis of these mutant lysozymes as well as a related ... | 1999 | 10512706 |
| assessment of extensive surgery for locally advanced lung cancer. safety and efficacy of induction therapy. | locally advanced lung cancer has a poor prognosis, despite extensive surgery conducted in an effort to improve survival. we evaluated the safety and efficacy of induction therapy prior to extensive surgery for locally advanced lung cancer. | 1999 | 10513134 |
| [analysis of the long-term survived patients with p-n2 non-small cell lung cancer]. | we had 308 patients with p-n2 non-small cell lung cancer who were treated with pulmonary resection therapy from june 1975 to march 1994. we analysed 9 patients who could survive for 5 years and more after the operation. all of them were male and the mean age was 61.1 years. histologically, 2 patients had adenocarcinoma and 7 patients had squamous cell carcinoma. the clinical staging was t2n1m0 in 2 patients, t1n2m0 in one, t2n2m0 in 2, t3n2m0 in 2, and t4n2m0 in 2 patients. the histopathological ... | 1999 | 10513155 |
| comparison of ribcage and posteroanterior thoracic spine stiffness: an investigation of the normal response. | evaluation of the movement response to posteroanterior (pa) loads applied to the spinous processes is a recognized part of the physical examination of the thoracic spine. during this clinical procedure the thoracic spine is supported by the ribcage which may contribute to the movement response. however, the contribution of ribcage stiffness to the pa stiffness of the thoracic spine has not been established. the purpose of this study was to measure the pa stiffness of the thoracic spine and compa ... | 1999 | 10513446 |
| effects of thyroid hormones on the release of calcitonin gene-related peptide (cgrp) by rat prostate glands in vitro. | it has been well known that calcitonin (ct) and calcitonin gene-related peptide (cgrp) are derived from the ct/cgrp gene which is localized in chromosome 11. cgrp is a 37-amino acid neuropeptide expressed predominantly in the nervous system and is one of the most potent endogenous vasodilatory peptides that have been found. only few reports described the distribution of cgrp in reproductive organs. moreover, the hormonal regulation of cgrp secretion is still not clear. the present study was desi ... | 1999 | 10513604 |
| [first-line chemo-radiotherapy neoadjuvant treatment in locally advanced (t4) epidermoid carcinoma of the esophagus]. | in the period 1993-1997 we performed two phase ii pilot studies of first-line chemo-radiotherapy in patients with locally advanced (t4) scc of the esophagus. the first protocol (3 cycles of ddp-vp16 + 45 gy) was used in 37 patients: toxicity was not negligible; a clinical tumor downstaging was obtained in 54% of cases; an r0 resection surgery was performed in 40% of patients. the overall median survival of the whole group of 37 patients was 11 months, while it was > 36 months for patients underg ... | 1999 | 10514923 |
| effect of divergent selection for total plasma phosphorus on plasma and yolk very low density lipoproteins and plasma concentrations of selected hormones in laying japanese quail. | japanese quail lines were divergently selected over 32 generations for laying hen plasma yolk precursor, as measured by total plasma phosphorus (tpp). the high (hp) and low (lp) lines were developed from a randombred control population (r1) that was maintained without conscious selection. the purpose of the present study was to characterize the composition of very low density lipoproteins (vldl) in laying japanese quail hens (vldly) and the concentration of selected hormones in laying hens from ... | 1999 | 10515352 |
| gene silencing-mediated resistance in transgenic tobacco plants carrying potato virus y coat protein gene. | unlike other pathogens, plant viruses are hardly controlled by chemical agents. potato virus y (pvy) is distributed around the world, and causes a great loss economically. in an attempt to minimize the damage by viruses, the pvy coat protein (cp) gene was introduced into tobacco by agrobacterium-mediated transformation. a significant proportion of the transgenic plants displayed resistance to pvy and showed substantially decreased cp transgene expression at both protein and steady-state mrna lev ... | 1999 | 10515600 |
| [discrimination and specific degradation of mrnas: a new turn with t4 phage gene silencing]. | | 1999 | 10517104 |
| streptomyces genomes: circular genetic maps from the linear chromosomes. | streptomyces chromosomes are linear dna molecules and yet their genetic maps based on linkage analysis are circular. the only other known examples of this phenomenon are in the bacteriophages t2 and t4, the linear genomic sequences of which are circularly permuted and terminally redundant, and in which replication intermediates include long concatemers. these structural and functional features are not found in streptomyces. instead, the circularity of streptomyces genetic maps appears to be caus ... | 1999 | 10517574 |
| [correlation of ultrasound and radioisotope studies in subacute de quervain thyroiditis]. | the first report on ultrasonic findings in subacute thyroiditis was published in 1975 (1). more detailed studies combining ultrasonographic and clinical findings are still missing. the present paper compares echostructure of the thyroid gland with the level of hormones--triiodthyronine (t3), thyroxine (t4), thyroidstimulating hormone (tsh) and thyroglobulin (tg) and thyroglobulin autoantibodies (tg-ab). | 1999 | 10518399 |
| capselect: a highly sensitive method for 5' cap-dependent enrichment of full-length cdna in pcr-mediated analysis of mrnas. | here we present capselect as a novel experimental approach for the selective enrichment of full-length cdnas in pcr-mediated analysis of mrna sequences. the method combines the 5'-cap-dependent addition of specifically three to four non-templated dcmp residues to the 3'-end of full-length cdnas by reverse transcriptases in the presence of manganese and the controlled ribonucleotide tailing of cdna ends by terminal deoxynucleotidyl transferase using ratp. by virtue of the generated terminal seque ... | 1999 | 10518626 |
| [delayed toxicity of brief preoperative irradiation and risk-adjusted postoperative radiotherapy of operative rectal carcinoma. results of a randomized prospective study]. | analysis of a randomized study of preoperative radiation therapy for operable carcinoma of the rectum with regard to late sequelae. results of tumor control and survival, which have already been published in detail are summarized for comparison and for confirmation of the conclusions. | 1999 | 10518976 |
| tumor-doubling time and onset of pulmonary metastasis from adenoid cystic carcinoma of the salivary gland. | adenoid cystic carcinoma (acc), an uncommon malignancy in the head and neck region, invades diffusely and often metastasizes to the lung, although the growth rate is very slow. a retrospective study was conducted in 30 patients with acc to ascertain the frequency of pulmonary metastasis, the doubling time of metastatic tumor deposits, and the time of onset for pulmonary metastasis. the following results were obtained: (1) of 30 patients with acc, 21 had pulmonary metastases (4 initially and 17 d ... | 1999 | 10519758 |
| effects of induced hypothyroidism on ovarian response to superovulation in brahman (bos indicus) cows. | to evaluate the effects of hypothyroidism on ovarian function, multiparous, nonlactating brahman cows (n = 18) were assigned randomly to dietary treatments containing either 0 (c; n = 9) or 4 mg x kg bw(-1) x d(-1) 6-n-propyl-2-thiouracil (ptu; n = 9), to suppress thyroid function, in the feed concentrate. weekly changes in bw and body condition score (bcs) were recorded. dietary treatments began on d 10 of the estrous cycle. ten days after the first treatment estrus, all cows received daily i.m ... | 1999 | 10521037 |
| the relative influence of vertebral body and intervertebral disc shape on thoracic kyphosis. | the aim of this study was to quantify the morphology or shape of thoracic vertebral bodies and intervertebral discs, and to examine the ex vivo association of thoracic kyphosis with these shape parameters. | 1999 | 10521627 |
| thyroid function, morphology and prevalence of thyroid disease in a population-based study of danish centenarians. | to investigate thyroid function, morphology, and autoimmunity in relation to physical function in an unselected population of centenarians. | 1999 | 10522958 |
| early, late symptoms and histological type of nasopharyngeal carcinoma. | after a wide literature review, we retrospectively analyzed the accurately recorded early onset and late symptoms at first diagnosis of nasopharyngeal carcinoma (npc) in a series of 85 patients. thirty-seven (44%) and 48 (56%) of cases had a squamous (scc) and undifferentiated (ucnt) histological npc subtype, respectively. thirteen (15%), 21 (25%) and 51 (60%) of cases were t2, t3 and t4, respectively. the involvement of locoregional lymph nodes resulted significantly more frequent in ucnt with ... | 1999 | 10523718 |
| hydrogastric sonography in the preoperative staging of gastric cancer. | depth of wall invasion is the main prognostic factor in gastric cancer. we studied the utility of hydrogastric sonography in the evaluation of transmural infiltration by gastric cancer. | 1999 | 10525211 |
| [renal tumor embolization]. | today the relevance of renal tumor embolization is not determined only by the technical and clinical success of the method. progress in diagnosis of early stages of renal carcinomas as well as the improvement of both surgical techniques and anesthetic procedures have lead to a change in the selection of patients for embolization. preoperative embolization of advanced renal cell carcinomas with tumor thrombus into the vena cava or of t4 tumors is now an established clinical procedure. the complet ... | 1999 | 10525637 |
| [thyroid autoantibodies in depressive disorders]. | whereas clinical relevant hypo- as well as hyperthyreosis are strongly suspected to induce psychiatric symptoms, there is a controversy about the relevance of only subclinical and autoimmune findings. we found autoantibodies (mak, tak, trak) in a high percentage (100 out of 144 = 70%) in severely depressed inpatients. also we found a hashimoto thyreoiditis in 5 patients. in the long run this may lead to relevant hypothyreosis which is regarded to be a risk factor for depression and for possible ... | 1999 | 10526630 |
| [administration of thyroid hormones in therapy of psychiatric illnesses]. | this review tries to give the state of the art of the therapeutic use of thyroid hormones in psychiatric disorders, mainly in depression. four hypotheses suggest an effect in these ailments: 1) a local relative t4 excess present (or better postulated to be present) in the brain of depressed patients is lowered by triiodothyronine (t3), by lowering serum levels of thyroxine (t4), 2) the effect of a depression-mediated cerebral lack of catecholamines is compensated by the t3/t4 induced activation ... | 1999 | 10526631 |
| [thyroid gland and sleep]. | a set of data suggests that the thyroid gland plays a role in the bi-directional interaction between the electrophysiological and the endocrine components of sleep, e.g. the nonrem-rem-cycle and the patterns of nocturnal hormone secretion, respectively. in detail thyroid-stimulating hormone (tsh) and thyroxin (t4) show circadian rhythms. a specific relationship was observed between tsh and rem sleep. blunted tsh levels were found in healthy elderly subjects and, probably due to overactivity of c ... | 1999 | 10526632 |
| transoral carbon dioxide laser resection of supraglottic carcinoma. | between 1981 and 1994, 34 patients with squamous cell carcinoma of the supraglottis were treated by transoral carbon dioxide laser resection, 12 of them palliatively. additional treatment included neck dissection in 21 patients and radiotherapy in 24 patients. the 3-year overall survival was 62%, and the actuarial survival 80%. the overall survival for t1 and t2 tumors was 71%, and that for t3 and t4 tumors was 47%. the overall 3-year survival for the early stages, i and ii, was 88%, and that fo ... | 1999 | 10527270 |
| pre-operative chemoradiotherapy in locally advanced rectal cancer. | the aim of the present study was to investigate the effectiveness and toxicity of pre-operative chemoradiation in locally advanced rectal cancer (t3-t4). | 1999 | 10527353 |
| hypothalamic-pituitary-thyroid axis status of humans during development of ageing process. | we have investigated hypothalamic-pituitary-thyroid function in four groups of healthy elderly male humans. group a (n=18, age range 20-45 years) served as healthy younger controls, group b (n=10, age range 50-60 years), group c (n=15, age range 60-70 years) and group d (n=16, age range 70-85 years) are the subjects of this study. groups c and d showed significantly lower t3 and thyroid-stimulating hormone (tsh), and higher t4 levels with respect to controls. evidence for tsh circadian modulatio ... | 1999 | 10529465 |
| serum cytokeratin 19 fragment levels in non-small cell lung cancer patients according to t factor in the tnm classification. | we examined changes in cytokeratin 19 fragment (cyfra 21-1) levels in relation to the t factor in 64 non-small cell n2m0 lung cancer patients. although a correlation between the levels and t factor was found (rho=0.627, p<0.0001), there was no difference between the levels in t3 and t4. serum cyfra 21-1 levels increased in the order of the following groups: the limited tumor group (t1+t2: n=28), the group with tumor extending to the pleura or chest wall (t3: n=13), and the group with tumor invad ... | 1999 | 10529467 |
| isolation of bacteria from cervical lymph nodes in patients with oral cancer. | thirty patients with oral mucosal cancer were studied in relation to oral mucosal damage and bacterial translocation to the regional lymph nodes in the neck. all 30 patients (gingiva 11, tongue 13, cheek mucosa four, oral floor two) underwent extensive, clean-contaminated, head-and-neck surgery (including neck dissection) with free flap reconstruction. a total of 153 lymph nodes was harvested for microbial and histological examination. viable bacteria were isolated from 70 lymph nodes (45.8%) fr ... | 1999 | 10530911 |
| development of motoneurons and primary sensory afferents in the thoracic and lumbar spinal cord of the south american opossum monodelphis domestica. | the postnatal development of the primary sensory afferent projection to the thoracic (t4) and lumbar (l4) spinal cord of the marsupial species monodelphis domestica was studied by using anterograde and retrograde neuronal tracers. large numbers of primary afferents and motoneurons were labelled by application of the carbocyanine dye dii into individual dorsal root ganglia (drg) afferents in short-term organ cultures. dorsal root axons had entered the cord at birth, but most primary afferent inne ... | 1999 | 10531537 |
| clinical relevance of the histological classification of sinonasal intestinal-type adenocarcinomas. | to verify the prognostic value of the histological typing of intestinal-type adenocarcinoma (itac) of the sinonasal tract, 41 such lesions were separately classified by 2 pathologists both according to the world health organization (who) criteria for the histological typing of large intestine adenocarcinomas (well, moderately, poorly differentiated adenocarcinomas, and mucinous adenocarcinomas) and to the criteria of kleinsasser and schroeder (papillary-tubular cylinder cell type, alveolar-goble ... | 1999 | 10534159 |
| [effect of combined administration of trh and dexamethasone in pregnant women and the course of their pregnancy]. | extremely immature neonates are threatened during the first days after delivery by many conditions which are due to incomplete development.--a key role is played during the first days of extrauterine life by the incidence and degree of the respiratory distress syndrome (rds). its incidence in neonates born before the completed 32nd week of gestation is very common. causal treatment of rds is not known. to overcome it the neonatologist must use in the majority of infants invasive techniques of co ... | 1998 | 10535166 |
| video-assisted thoracic diskectomy and anterior release: a biomechanical analysis of an endoscopic technique. | this study evaluates the residual biomechanical stability of the spine following multilevel anterior diskectomies and anterior longitudinal ligament release using video-assisted thoracoscopic surgery (vats). eighteen domestic pigs were randomly divided into three groups of six pigs. group 1 underwent thoracic anterior release from t4-t9 using a left-sided vats approach, group 2 underwent thoracic anterior release from t4-t9 via a traditional left thoracotomy (open), and group 3 did not undergo s ... | 1999 | 10535554 |
| increased glucose synthesis in renal tubule fragments from hyperthyroid rats. | rates of glucose synthesis from several substrates were examined in renal tubule fragments from hyperthyroid rats. a hyperthyroid state was induced by daily intraperitoneal injections of thyroxine (t4) (100 microg/100 g body weight) for 14 days. at the end of the experimental period, plasma triiodothyronine and t4 levels were six and eight times higher, respectively, than initial values. hyperthyroid rats gained less weight and had lower blood glucose despite an increased food intake. in both co ... | 1999 | 10535706 |
| dna packing in stable lipid complexes designed for gene transfer imitates dna compaction in bacteriophage. | the structure of complexes made from dna and suitable lipids (lipoplex, lx) was examined by cryo-electron microscopy (cryoem). we observed a distinct concentric ring-like pattern with striated shells when using plasmid dna. these spherical multilamellar particles have a mean diameter of 254 nm with repetitive spacing of 7.5 nm with striation of 5.3 nm width. small angle x-ray scattering revealed repetitive ordering of 6.9 nm, suggesting a lamellar structure containing at least 12 layers. this co ... | 1999 | 10535915 |
| opening of a monomer-monomer interface of the trimeric bacteriophage t4-coded gp45 sliding clamp is required for clamp loading onto dna. | the replication system of bacteriophage t4 uses a trimeric ring-shaped processivity clamp (gp45) to tether the replication polymerase (gp43) to the template-primer dna. this ring is placed onto the dna by an atpase-driven clamp-loading complex (gp44/62) where it then transfers, in closed form, to the polymerase. it generally has been assumed that one of the functions of the loading machinery is to open the clamp to place it around the dna. however, the mechanism by which this occurs has not been ... | 1999 | 10535942 |
| comparison of a new piezoelectric train-of-four neuromuscular monitor, the paragraph, and the relaxometer mechanomyograph. | the paragraph is a new device for monitoring neuromuscular function using a piezoelectric motion sensor. in 20 patients, monitoring of neuromuscular block produced by cisatracurium 0.1 mg kg-1 was compared using the paragraph and a relaxometer 2 mechanomyograph. the paragraph was quick to set up, and easy to operate and interpret. there were no significant differences in the time to 100% depression of t1/t0, time to 25% recovery of t1/t0 or time to recovery of t1/t0 from 25% to 75%, measured by ... | 1999 | 10536564 |
| thyroid hormone regulates the extracellular organization of laminin on astrocytes. | astrocytes produce laminin, a key extracellular matrix guidance molecule in the developing brain. laminin is bound to transmembrane receptors on the surface of astrocytes known as integrins, which are, in turn, bound to the microfilament meshwork inside the astrocyte. previous studies have shown that t4 regulates the pattern of integrin distribution in astrocytes by modulating the organization of the microfilaments. in this study, the effect of thyroid hormone on the secretion and topology of la ... | 1999 | 10537126 |
| type 3 iodothyronine deiodinase is selectively expressed in areas related to sexual differentiation in the newborn rat brain. | thyroid hormone (t4 and t3) concentrations in target tissues are greatly influenced by the activity of iodothyronine deiodinases. type 1 and 2 deiodinases generate t3 from t4, while deiodinase type 3 (d3) transforms t4 and t3 to inactive metabolites. coordination of the expression and activity of these enzymes is postulated to play an important role in physiology and development, making it possible that individual cells and tissues regulate the concentrations of the active hormone according to s ... | 1999 | 10537178 |
| long-term results of radiotherapy alone for carcinoma of the vulva. | from 1953 to 1978, definitive radiotherapy for carcinoma of the vulva without surgery was used as a standard therapy at a university hospital in germany. we retrospectively reviewed the records of 170 patients treated mainly with electron-beam radiation to the vulva and co-60 to the inguinal lymph nodes. total doses to the vulva ranged from 6 to 90 gy. a 30-year retrospective follow-up was accomplished. tumor and node classification, tumor location, and parity were significant independent factor ... | 1999 | 10539381 |
| relationship between stimulating current and accelographic train-of-four response at the great toe. | we investigated the accelographic train-of-four response evaluated at the great toe at varying stimulating currents. fifteen adult patients undergoing elective general anaesthesia were studied. the mean current at which a supramaximal t1 value could be elicited was > 49 (9) ma [mean (sd)]. ratios of accelographic t1 values at 50, 40, 30, 20 and 10 ma to accelographic t1 value at 60 ma were 0.90 (0. 18), 0.58 (0.37), 0.38 (0.37), 0.19 (0.26) and 0.00 (0.00), respectively [mean (sd), p < 0.05 for ... | 1999 | 10540101 |
| the energetics of t4 lysozyme reveal a hierarchy of conformations. | we have used native state exchange to examine the energy landscape of the well-characterized protein t4 lysozyme. although the protein exhibits two-state behavior by traditional probes, the energy landscape determined here is much more complex. the average stability of the c-terminal subdomain is significantly higher than that for the n-terminus suggesting at least two regions of unfolding. at a more detailed level, there appears to be a broad continuum of stabilities throughout each region. the ... | 1999 | 10542101 |
| identification of the rna binding domain of t4 rega protein by structure-based mutagenesis. | the t4 translational repressor rega protein folds into two structural domains, as revealed by the crystal structure (kang, c.-h. , chan, r., berger, i., lockshin, c., green, l., gold, l., and rich, a. (1995) science 268, 1170-1173). domain i of the rega protein contains a four-stranded beta-sheet and two alpha-helices. domain ii contains a four-stranded beta-sheet and an unusual 3/10 helix. since beta-sheet residues play a role in a number of protein-rna interactions, one or both of the beta-she ... | 1999 | 10542265 |
| incidence of sideropenia and effects of iron repletion treatment in women with subclinical hypothyroidism. | sideropenia affects ca. 20% of the world population, and iron dependent anemia is the most frequent type of anemia worldwide. the aim of the study was to investigate the incidence of sideropenia and dependent anemia in patients with subtle changes of the thyroid function, such as subclinical hypothyroidism (sh). 57 women with sh and 61 euthyroid controls (cg) were studied. serum concentrations of t4, t3, tsh, anti-tpo, anti-tg, ferrum (fe), ferritin (frt) total iron binding capacity (tibc) and b ... | 1999 | 10543412 |
| preoperative chemoradiotherapy and surgery for selected non-small cell lung cancer iiib subgroups: long-term results. | preoperative chemoradiotherapy is feasible for selected patients with non-small cell lung cancer stage iiib. the aim of this investigation was to analyze long-term results after this multimodality approach and to identify subgroups with improved long-term prognosis. | 1999 | 10543470 |
| video assistance reduces complication rate of thoracoscopic sympathicotomy for hyperhidrosis. | thoracoscopic sympathicotomy has proved successful in the treatment of palmar hyperhidrosis. however, up to 8% of patients experience horner's syndrome, and about 50% show compensatory sweating. this study evaluates the role of video assistance in thoracoscopic sympathicotomy for primary hyperhidrosis of the upper limb. | 1999 | 10543476 |
| specific inhibition of the eubacterial dna ligase by arylamino compounds. | all known dna ligases catalyze the formation of a phosphodiester linkage between adjacent termini in double-stranded dna via very similar mechanisms. the ligase family can, however, be divided into two classes: eubacterial ligases, which require nad(+) as a cofactor, and other ligases, from viruses, archaea, and eukaryotes, which use atp. drugs that discriminate between dna ligases from different sources may have antieubacterial activity. we now report that a group of arylamino compounds, includ ... | 1999 | 10543760 |
| polymerization of bacteriophage t4 tail sheath protein mutants truncated at the c-termini. | gene 18 of bacteriophage t4 encodes the contractile protein of the tail sheath. previous work has shown that the full-length recombinant gene product (gp) 18 of 658 amino acid residues assembles in escherichia coli cells into a long polysheath structure. however, the gp18 mutants truncated at the n-termini form insoluble aggregates similar to inclusion bodies. in this study, six plasmid vectors expressing the recombinant gp18 proteins truncated at the c-termini have been constructed. the cdelta5 ... | 1999 | 10544047 |
| role of the c-terminal rdel motif of the myxoma virus m-t4 protein in terms of apoptosis regulation and viral pathogenesis. | the purpose of this study was to investigate the significance of the c-terminal rdel motif of the myxoma virus m-t4 protein in terms of apoptosis regulation and role in viral virulence. to accomplish this, a recombinant myxoma virus was created in which the c-terminal rdel motif of m-t4 was deleted and a selectable marker (ecogpt) was inserted immediately downstream. we hypothesized that removal of the rdel motif from m-t4 would alter the subcellular localization of the protein and provide insig ... | 1999 | 10544103 |
| atp/adp switches the higher-order structure of dna in the presence of spermidine. | in the living cellular environment, dnas exist in a compact state in the presence of a polyamine, such as spermidine. we found that the hydrolysis of atp into adp induces the folding of elongated dnas, by the single-chain observation of individual t4 dna molecules. this result is discussed in relation to the possible role of atp as a regulatory factor in genetic activity, in addition to its well-established role as an energy source. | 1999 | 10544259 |
| methionine and alanine substitutions show that the formation of wild-type-like structure in the carboxy-terminal domain of t4 lysozyme is a rate-limiting step in folding. | in an attempt to identify a systematic relation between the structure of a protein and its folding kinetics, the rate of folding was determined for 20 mutants of t4 lysozyme in which a bulky, buried, nonpolar wild-type residue (leu, ile, phe, val, or met) was substituted with alanine. methionine, which approximated the size of the original side chain but which is of different shape and flexibility, was also substituted at most of the same sites. mutations that substantially destabilize the prote ... | 1999 | 10545167 |
| identification of in vivo induced genes in actinobacillus pleuropneumoniae. | we have developed an in vivo expression technology (ivet) system to identify actinobacillus pleuropneumoniae gene promoters that are specifically induced in vivo during infection. this system is based upon an avirulent riboflavin-requiring a. pleuropneumoniae mutant and a promoter-trap vector (ptf86) that contains, in sequence, the t4 terminator, a unique bam hi site, a promoterless copy of the v. harveyi luxab genes, and a promoterless copy of the b. subtilis ribbah genes in the e. coli - a. pl ... | 1999 | 10545257 |
| the structure of bacteriophage t4 gene product 9: the trigger for tail contraction. | the t4 bacteriophage consists of a head, filled with double-stranded dna, and a complex contractile tail required for the ejection of the viral genome into the escherichia coli host. the tail has a baseplate to whïch are attached six long and six short tail fibers. these fibers are the sensing devices for recognizing the host. when activated by attachment to cell receptors, the fibers cause a conformational transition in the baseplate and subsequently in the tail sheath, which initiates dna ejec ... | 1999 | 10545330 |
| the cooperativity of burst phase reactions explored. | the denaturant-dependence of the major, observable relaxation rates for folding (kobs) of ribonuclease hi from escherichia coli (rnase h) and phage t4 lysozyme (t4l) reveal that, for both proteins, folding begins with the rapid and transient accumulation of intermediate species in a "burst phase" which precedes the rate-limiting formation of the native state; this is evidenced by a "rollover" in the folding limb of the rate profiles (kobs versus denaturant, or chevron plot). these rate profiles ... | 1999 | 10547295 |
| treatment of maxillary sinus carcinoma: a comparison of the 1997 and 1977 american joint committee on cancer staging systems. | this study was conducted to assess the effectiveness of the 1997 american joint committee on cancer (ajcc) staging system to predict survival and local control of patients with maxillary sinus carcinoma and to identify significant factors for overall survival, local control, and distant metastases in patients with these tumors. | 1999 | 10547542 |
| surgical and multimodal approaches to cancer of the oesophagus: state of the art. | this review article aims to discuss the modalities of oesophageal resection, to define the categories of patients who are most likely to benefit from oesophagectomy with extensive lymph node clearance, and to analyse the eventual contribution of nonsurgical neo-adjuvant or adjuvant therapies to improving long-term survival rates achieved by surgery alone. both the review of the literature devoted to potentially curative treatment of oesophageal cancer and the authors' own experience indicate tha ... | 1999 | 10547892 |
| successful weaning from cardiopulmonary bypass with central venous prostaglandin e1 and left atrial norepinephrine infusion in patients with acute pulmonary hypertension. | postoperative pulmonary hypertension increases the mortality risk in cardiac surgery. we have used central venous prostaglandin e1 (pge1) and left atrial norepinephrine (ne) infusion to wean from cardiopulmonary bypass (cpb) patients with refractory postoperative pulmonary hypertension. | 1999 | 10548203 |
| [neuromuscular recovery following mivacurium is predictable in patients with severe systemic disease prognoses]. | fast recovery from mivacurium-induced neuromuscular blockade is impaired in patients with decreased plasma cholinesterase activity which is often associated with dysfunction of different organs. nevertheless, predictability of neuromuscular recovery may be given. thus, this study evaluates parameters to predict individual neuromuscular recovery in patients with uncommon diseases. | 1999 | 10548961 |
| joining rna molecules with t4 dna ligase. | | 1999 | 10549511 |
| gemcitabine and cisplatin versus mitomycin, ifosfamide, and cisplatin in advanced non-small-cell lung cancer: a randomized phase iii study of the italian lung cancer project. | to compare gemcitabine and cisplatin (gc) with mitomycin, ifosfamide, and cisplatin (mic) chemotherapy in patients with stage iiib (limited to t4 for pleural effusion and n3 for supraclavicular lymph nodes) or stage iv non-small-cell lung cancer (nsclc). the end points were the evaluation of quality of life (qol), response rates, survival, and toxicity. | 1999 | 10550150 |
| vibriophage kvp40 and coliphage t4 genomes share a homologous 7-kbp region immediately upstream of the gene encoding the major capsid protein. | vibriophage kvp40, a large tailed dna phage morphologically similar to t-even coliphages, has a major capsid protein (mcp) homologous to the equivalent protein, gp23(*), of coliphage t4. the sequence analysis was extended to a 7-kbp region immediately upstream of the mcp gene encoding the precursor of mcp. the region as a whole was fairly homologous to the corresponding region of the t4 genome and contained 8 orfs homologous to t4 genes 17, 18, 19, 20, 67, 68, 21, and 22 in the same order as in ... | 1999 | 10550672 |
| wakame seaweed suppresses the proliferation of 7,12-dimethylbenz(a)-anthracene-induced mammary tumors in rats. | we examined the anti-tumor proliferation effects of wakame seaweed on 7,12-dimethylbenz(a)-anthracene (dmba)-induced rat mammary tumor. dmba was administered to 8-week-old female sprague-dawley rats, and rats which developed mammary tumors were assigned randomly to three groups. commercial rat feed was used in a control group (group i-a), and two feed mixtures were prepared, which contained commercial rat feed blended with wakame at 1.0% (group i-b) and 5.0% (group i-c) by weight. the respective ... | 1999 | 10551319 |
| high thoracic epidural anesthesia does not inhibit sympathetic nerve activity in the lower extremities. | sympathetic nerve activity was recorded in the leg during high thoracic epidural anesthesia with a segmental sensory blockade of the upper thoracic dermatomes to test the hypothesis that the sympathetic blockade accompanying thoracic epidural anesthesia includes caudal parts of the sympathetic nervous system. | 1999 | 10551580 |
| the analgesic action of nitrous oxide is dependent on the release of norepinephrine in the dorsal horn of the spinal cord. | the authors and others have demonstrated that supraspinal opiate receptors and spinal alpha2 adrenoceptors are involved in the analgesic mechanism for nitrous oxide (n2o). the authors hypothesize that activation of opiate receptors in the periaqueductal gray results in the activation of a descending noradrenergic pathway that releases norepinephrine onto alpha2 adrenoceptors in the dorsal horn of the spinal cord. | 1999 | 10551592 |
| characterization of the thyroxine-binding site of thyroxine-binding globulin by site-directed mutagenesis. | the principal transport protein for t4 in human blood, thyroxine-binding globulin (tbg), binds t4 with an exceptionally high affinity (ka = 10(10) m(-1)). its homology to the superfamily of the serpins has recently been used in the design of chimeric proteins, providing experimental evidence that an eight-stranded beta-barrel domain encompasses the ligand-binding site. we have now characterized the t4 binding site by site-directed mutagenesis. sequence alignment of tbg from several species revea ... | 1999 | 10551780 |
| potato performance in response to different mulches. 1. nitrogen metabolism and yield. | this work evaluates the impact that the application of different mulches exerts on nitrogen (n) metabolism in roots and leaves and on yield of potato tubers (solanum tuberosum var. spunta). the parameters analyzed in this experiment were root temperature; nitrate reductase and nitrite reductase activities; concentrations of nitrates, ammonium, amino acids, proteins, and organic n; dry weight of roots and leaves; and tuber yield. use of white polyethylene (t2) and white-black plastic (t3) promote ... | 1999 | 10552541 |
| analysis of the hereditary pancreatitis-associated cationic trypsinogen gene mutations in exons 2 and 3 by enzymatic mutation detection from a single 2.2-kb polymerase chain reaction product. | hereditary pancreatitis is associated with at least 2 mutations in the cationic trypsinogen gene. the purpose of the present study is to test the utility of t4 endonuclease vii for the detection of cationic trypsinogen r117h mutations. in addition, the possibility of screening for r117h, n21i, and a8v mutations in a single 2.2-kb polymerase chain reaction (pcr) product using t4 endonuclease vii was investigated. | 1999 | 10553021 |