| histology and ultrastructure of the lymph nodes of the buffalo (bos bubalus). | pre-scapular, femoral and mesenteric lymph nodes from five buffalo calves and five buffalo bulls were studied using light and transmission electron microscopy. the nodes were surrounded with a thin capsule of dense connective tissue and smooth muscles. subcapsular and trabecular lymphatic sinuses were lined with endothelial cells resting on a basement membrane. the cortex was formed by lymphoid follicles and inter-follicular lymphocytes. primary and secondary follicles were observed. the medulla ... | 2015 | 24810142 |
| first cloned swamp buffalo produced from adult ear fibroblast cell. | the world's first cloned swamp buffalo (bubalus bubalis) derived from adult ear skin fibroblast has been reported. donor fibroblast cells were produced from biopsies taken from adult male ear skin and in vitro matured oocytes obtained from a slaughterhouse were used as cytoplasts. a total of 39 blastocysts and 19 morulae fresh embryos were transferred into 12 recipient buffaloes. progesterone assays indicated establishment of pregnancy in 10 of the 12 buffaloes (83.3%) after 45 days, with six an ... | 2014 | 24804579 |
| effects of melatonin and epiphyseal proteins on fluoride-induced adverse changes in antioxidant status of heart, liver, and kidney of rats. | several experimental and clinical reports indicated the oxidative stress-mediated adverse changes in vital organs of human and animal in fluoride (f) toxicity. therefore, the present study was undertaken to evaluate the therapeutic effect of buffalo (bubalus bubalis) epiphyseal (pineal) proteins (bep) and melatonin (mel) against f-induced oxidative stress in heart, liver, and kidney of experimental adult female rats. to accomplish this experimental objective, twenty-four adult female wistar rats ... | 2014 | 24790596 |
| association of a novel snp in exon 10 of the igf2 gene with growth traits in egyptian water buffalo (bubalus bubalis). | insulin-like growth factor 2 (igf2) plays an important role in muscle growth and it might be used as a marker for the growth traits selection strategies in farm animals. the objectives of this study were to detect polymorphisms in exon 10 of igf2 and to determine associations between these polymorphisms and growth traits in egyptian water buffalo. pcr-single-strand conformation polymorphism (sscp) and dna sequencing methods were used to detect any prospective polymorphism. a novel single nucleot ... | 2014 | 24756464 |
| ontogenic development of corticotrophs in fetal buffalo (bubalus bubalis) pituitary gland. | to evaluate the subpopulation of corticotrophs in developing buffalo (bubalus bubalis) fetus, recovered pituitary glands (n=6 per group) from late first, second and third gestational female buffalo dams. the corticotrophs were identified by using specific antibodies against proopiomelanocortin (pomc) and adrenocorticotrophic hormone (acth) through immunohistochemistry. there was a significant (p≤0.05) increase of immunoreactive (ir) ir-acth cells during late 2nd trimester while, ir-pomc cells we ... | 2014 | 24704996 |
| haematological alterations induced by oral subacute exposure to fenvalerate, nitrate and their combination in domestic buffalo, bubalus bubalis. | the present study investigated haematological alterations induced by oral subacute exposure to fenvalerate, nitrate and their combination in the domestic buffalo, bubalus bubalis. fenvalerate exposure produced significant declines in haemoglobin (hb), total leukocyte count (tlc), total erythrocyte count (tec) and mean corpuscular haemoglobin concentration (mchc), and a corresponding elevation in mean corpuscular volume (mcv). following oral exposure to sodium nitrate, significant declines in blo ... | 2014 | 24687223 |
| lead and trace element levels in milk and blood of buffaloes (bubalus bubalis) from hyderabad, india. | the presence of lead (pb) in milk and its interaction with trace elements is a serious health concern. present study is aimed at determining pb and trace element (fe, zn and mg) levels in milk and blood/serum samples of lactating buffaloes (bubalus bubalis) living in a market-area (group-a) and a dairy-experimental station (group-b), hyderabad, india. in addition, kidney and liver function tests were assessed. fodder, milk and blood pb levels were significantly (p < 0.01) higher in group-b. elev ... | 2014 | 24671305 |
| stage specific expression of atp-binding cassette and solute carrier superfamily of transporter genes in mammary gland of riverine buffalo (bubalus bubalis). | in the present study, expression level of various atp-binding cassette (abc) viz., abca1, abca7, abcg1, abcg2, and abcg5; associated transcription factors viz., srebf1, lxrα (nr1h3), ppara, and solute carriers (slc); or glucose transporters (glut) viz., slc2a1(glut1), slc2a4 (glut4), slc2a8 (glut8), and slc2a12 (glut12) superfamily of transporters were compared across physiological stages of buffalo mammary gland. the relative expression of abca1, and abcg1 was significantly (p < 0.05) higher in ... | 2014 | 24669870 |
| testicular lesions and epididymal sperm parameters in the iranian river buffalo (bubalus bubalis). | the aim of this study was to evaluate the testicular lesions and their effects on the epididymal sperm parameters in the iranian river buffalo (bubalus bubalis). total numbers of 117 scrota from the pubertal buffalo were provided from the local slaughterhouse. the samples were evaluated for morphological parameters and any macro- or microscopic lesions. the sterile swabs from the testis parenchyma were subjected to microbiology culture. the epididymal spermatozoon was analysed for concentration, ... | 2015 | 24661073 |
| mammary diffuse fibroadenomatoid hyperplasia in water buffalo (bubalus bubalis): three cases. | the current report describes 3 rare cases of mammary diffuse fibroadenomatoid hyperplasia in water buffalo (bubalus bubalis). all of the animals were between 10 and 12 months of age. grossly, the lesions consisted of severe diffuse swelling with homogeneous large masses in the udder. surgical removal of the masses was curative. microscopically, there was severe hyperplasia of the mammary epithelium and numerous well-differentiated and mildly pleomorphic acini and their associated ducts. moderate ... | 2014 | 24621849 |
| prevalence and molecular characterization of sarcocystis species in water buffaloes (bubalus bubalus) in egypt. | the present study was planned to investigate the prevalence of sarcocystis spp. among slaughtered water buffaloes (bubalus bubalis) at alexandria province, egypt. three hundred blood samples were collected from slaughtered buffaloes (5-7 years old). two techniques were used to evaluate the seroprevalence of sarcocystis spp., enzyme-linked immunosorbent assay (elisa) and indirect haemagglutination assay (iha). it was revealed that 203 (67.6 %) and 191 (63.6 %) of the tested serum samples were ser ... | 2014 | 24619513 |
| synchronization of follicular wave emergence following ultrasound-guided transvaginal follicle ablation or estradiol-17β administration in water buffalo (bubalus bubalis). | the aim of this study was to assess the synchrony in follicular wave emergence and subsequent ovulation following dominant follicle ablation or estradiol-17β administration. six cycling murrah buffaloes were sequentially allotted to three groups, that is, control, follicular ablation, and estradiol-17β groups. for the control group, buffaloes at random stages of estrous cycle were examined daily by transrectal ultrasonography for 14 days and the day of wave emergence was recorded. following indu ... | 2014 | 24612954 |
| effect of extender and equilibration time on post thaw motility and chromatin structure of buffalo bull (bubalus bubalis) spermatozoa. | the aim of the present study was to investigate the effects of four equilibration times (2, 4, 8 and 16 hours) and two extenders (tris or bioxcell®) on cryopreservation of buffalo semen. | 2014 | 24611139 |
| reprogramming of buffalo (bubalus bubalis) foetal fibroblasts with avian egg extract for generation of pluripotent stem cells. | an attempt was made to study the efficiency of chicken (gallus gallus domesticus) egg extract (ee) to reprogram buffalo (bubalus bubalis) foetal fibroblasts (bffs) without incorporation of ectopic transcription factors. the isolated bffs were cultured in media supplemented with 2%, 4%, 6% and 10% ee to induce reprogramming. it was observed that fewer but larger sized alkaline phosphatase positive (ap(+ve)) colonies developed in culture system containing 2% ee whereas, more but smaller sized colo ... | 2014 | 24602917 |
| insight into buffalo (bubalus bubalis) rig1 and mda5 receptors: a comparative study on dsrna recognition and in-vitro antiviral response. | rig1 and mda5 have emerged as important intracellular innate pattern recognition receptors that recognize viral rna and mediate cellular signals controlling type i interferon (ifn-i) response. buffalo rig1 and mda5 genes were investigated to understand the mechanism of receptor induced antiviral response. sequence analysis revealed that rig1 and mda5 maintain a domain arrangement that is common in mammals. critical binding site residues of the receptors are evolutionary conserved among mammals. ... | 2014 | 24587036 |
| short communication: effects of systemic treatment with penethamate hydriodide on udder health and milk yields in dry primiparous mediterranean buffaloes (bubalus bubalis). | the effects of penethamate hydriodide (mamyzin, boehringer ingelheim, ingelheim, germany) on udder health and milk yields were evaluated in primiparous mediterranean buffaloes (bubalus bubalis). an intramuscular administration of 10 million international units was performed in 20 buffaloes at 7 d precalving (treatment group; tg), and 20 animals were enrolled as the control group (cg). evening milk samplings were performed at 10, 30, and 60 d in milk (dim). somatic cell count (scc) values were ev ... | 2014 | 24565324 |
| production of wild buffalo (bubalus arnee) embryos by interspecies somatic cell nuclear transfer using domestic buffalo (bubalus bubalis) oocytes. | the objective of this study was to explore the possibility of producing wild buffalo embryos by interspecies somatic cell nuclear transfer (iscnt) through handmade cloning using wild buffalo somatic cells and domestic buffalo (bubalus bubalis) oocytes. somatic cells derived from the ear skin of wild buffalo were found to express vimentin but not keratin and cytokeratin-18, indicating that they were of fibroblast origin. the population doubling time of skin fibroblasts from wild buffalo was signi ... | 2014 | 24494649 |
| analysis of slc11a1 gene expression in healthy water buffalo (bubalus bubalis) blood cells using qpcr. | slc11a1 (solute carrier family 11 member 1 protein) gene influences the initial phase of bacterial cellular infections through macrophage activation. recent literature on buffalo has attempted to associate the genotype of the polymorphic microsatellite located in the 3'untranslated region (3'utr) of the gene, with either susceptibility to brucellosis or with improved macrophage function. carriers of the (gt)16 allele have been reported to be resistant to brucellosis. in this study we analyzed th ... | 2013 | 24391044 |
| glutathione-s-transferase: role in buffalo (bubalus bubalis) sperm capacitation and cryopreservation. | in this study, glutathione-s-transferase mu3 (gst) has been reported to play an important role in sperm capacitation, acrosome reaction, and fertilization. the freshly ejaculated buffalo spermatozoa were in vitro capacitated using heparin (10 μg/ml) or cryopreserved in egg yolk citrate extender. glutathione-s-transferase was identified and characterized in terms of their isozymic forms, tyrosine phosphorylation, and immunolocalization patterns in cryopreserved buffalo spermatozoa in comparison w ... | 2014 | 24388131 |
| embryonic development of heart in indian buffalo (bubalus bubalis). | the present study was conducted on 35 buffalo foetuses from 0.9 cm cvrl (32 days) to 99.5 cm cvrl (298 days) to observe the morphogenesis and histogenesis of heart. the study revealed that, in 0.9 cm cvrl buffalo foetus, heart was unseptated and tubular which was clearly divided into common atrial chamber dorsally, primitive ventricles ventrally, primitive outflow tract with bulbous cordis region proximally, and aortic sac distally at 1.2 cm cvrl. septum primum appeared at 1.9 cm cvrl whereas th ... | 2014 | 27355030 |
| tuberculosis prevalence and risk factors for water buffalo in pará, brazil. | the prevalence of and possible risk factors for tuberculosis were studied in water buffalo from pará, brazil. in this study, 3,917 pregnant and nonpregnant female murrah and mediterranean buffaloes were studied; 2,089 originated from marajó island, and 1,108 were from the mainland. the comparative cervical tuberculin test was used as a diagnostic test for tuberculosis in these animals. the prevalence of positive buffaloes was 3.5 % (100/2,809) on marajó island and 7.2 % (80/1,108) on the mainlan ... | 2014 | 24356890 |
| prevalence of subclinical coccidiosis in river buffalo calves of southwest of iran. | despite the importance of buffalo farming in iran, little is known in this country about the abundance and distribution of eimeria spp. in the animal species. the present study was designed to investigate the prevalence and species characterization of eimeria oocysts in river buffalo calves of khuzestan province, southwest of iran. of the total 108 fecal samples examined for eimeria, 108 (100%) were found infected with 11 species of the parasite. among the identified species of eimeria, e. bovis ... | 2013 | 24338314 |
| bacterial diversity associated with feeding dry forage at different dietary concentrations in the rumen contents of mehshana buffalo (bubalus bubalis) using 16s pyrotags. | pyrosequencing of 16s rrna gene targeting bacteria was applied to identify diet-induced shifts in the microbiome of both solid and liquid ruminal fractions retrieved from water buffalo fed different diets. the depth of coverage of metabolically active bacteria in a community using different primer pairs was also investigated. to assess reproducibility, animal to animal variation was considered in all phylogenetic and community comparisons. the experiment included four non-lactating water buffalo ... | 2014 | 24315806 |
| developmental competence of buffalo (bubalus bubalis) pluripotent embryonic stem cells over different homologous feeder layers and the comparative evaluation with various extracellular matrices. | use of somatic cells as a feeder layer to maintain the embryonic stem cells (escs) in undifferentiated state limits the stem cell research design, since experimental data may result from a combined escs and feeder cell response to various stimuli. therefore, present study was designed to evaluate the developmental competence of the buffalo escs over different homogenous feeders and compare with various extracellular matrices using different concentrations of lif. | 2013 | 24298371 |
| effect of flubendiamide, lead and their combined exposure on erythrocytic indices in water buffalo calves. | this study evaluated the effect of flubendiamide, lead and their combined oral administration on erythrocytic indices in water buffalo calves. exposure to flubendiamide alone resulted in non-significant decreases in hemoglobin (hb), packed cell volume (pcv), total erythrocyte count (tec), mean corpuscular volume (mcv) and mean corpuscular hemoglobin (mch). lead acetate exposure resulted in significant declines in hb, pcv and tec. combined exposure to flubendiamide and lead resulted in declines i ... | 2014 | 24292848 |
| expression and localization of locally produced growth factors regulating lymphangiogenesis during different stages of the estrous cycle in corpus luteum of buffalo (bubalus bubalis). | recent experiments using expression, immunolocalization, and cell culture approaches have provided leading insights into regulation of luteal angiogenesis by different growth factor systems and its role in the function of corpus luteum (cl) in buffalo. on the contrary, lymphangiogenesis and its regulation in the cl are still poorly understood. the aim of this study was to evaluate the expression and localization of lymphangiogenic factors (vascular endothelial growth factor [vegf]-c and vegfd), ... | 2014 | 24246422 |
| endocrine changes, timing of ovulation, ovarian follicular growth and efficacy of a novel protocol (estradoublesynch) for synchronization of ovulation and timed artificial insemination in murrah buffaloes (bubalus bubalis). | experiments were conducted to investigate (1) ovarian follicular growth, timing of ovulation and associated endocrine changes (progesterone, estrogen, and lh) in cycling, and (2) efficacy in terms of pregnancy rate in cycling and anestrus murrah buffaloes subjected to the estradoublesynch protocol (prostaglandin f2α [pgf2α] 0, gnrh 2, pgf2α 9, estradiol benzoate, eb 10). twelve cycling buffaloes were subjected to the estradoublesynch protocol and observed for ovulation, follicle size, and endocr ... | 2014 | 24125725 |
| arachidic acid in extender improves post-thaw parameters of cryopreserved nili-ravi buffalo bull semen. | cryopreservation process reduces lipids and phospholipids from buffalo bull spermatozoa. it was therefore hypothesized that supplementation of fatty acid to extender may improve the post-thaw quality of buffalo semen. the objective was to evaluate the effect of arachidic acid supplementation in extender on post-thaw quality of buffalo bull (bubalus bubalis) spermatozoa. semen was collected from three adult nili-ravi buffalo bulls of similar age group with artificial vagina (42°c) for 3 weeks (re ... | 2014 | 24112366 |
| carboxypeptidase-b from bubalus bubalis pancreas: purification, properties and maldi-tof monitored activation of proinsulin. | carboxypeptidase-b (e.c 3.4.17.2) catalyzes the hydrolysis of peptides and esters at c-terminus of arginine and lysine residues. our study describes the large scale purification, n-terminal sequence analysis and physiochemical properties of pancreatic enzyme from river buffalo (bubalus bubalis). the enzyme was purified up to 71 folds by anion-exchange chromatography with 21% final recovery. purified enzyme displayed two bands on sds-page with molecular weights of 9 kda and 26 kda respectively, t ... | 2013 | 24035945 |
| isolation and characterization of luteal cells in buffalo (bubalus bubalis). | the objective of the study was to evaluate the morphological and functional characteristics of luteal cells isolated from buffalo ovary. luteal cells exhibited columnar morphology and contact inhibition at the stage of confluence. protein concentrations increased linearly from day 3 to day 7 of culture. dna concentrations increased from day 3 to day 5 and then declined to day 7 of culture. pgf2alpha concentrations decreased progressively from day 3 to day 7 of culture. it was concluded that buff ... | 2013 | 24020092 |
| effect of feeding inorganic chromium on growth performance, endocrine variables, and energy metabolites in winter-exposed buffalo calves (bubalus bubalis). | we investigated the effect of chromium (cr) supplementation on the growth performance, energy metabolites, and hormonal variation in winter-exposed buffalo calves. twenty-four female buffalo calves were randomly allotted to four dietary treatments (n = 6) for a period of 120 days. feeding regimen was the same in all the groups, except the animals in the four respective groups were additionally supplemented with 0.0, 0.5, 1.0, and 1.5 mg of cr/kg dm in the form of crcl3.6h2o. calves were monitore ... | 2013 | 24013931 |
| treatment of donor cells with trichostatin a improves in vitro development and reprogramming of buffalo (bubalus bubalis) nucleus transfer embryos. | it has been reported that buffalo (bubalus bubalis) embryos reconstructed by somatic cell nucleus transfer (scnt) can develop to the full term of gestation and result in newborn calves. however, the developmental competence of reconstructed embryos is still low. recently, it has been reported that treating donor cells or embryos with trichostatin a (tsa) can increase the cloning efficiency in some species. thus, the present study was undertaken to improve the development of buffalo scnt embryos ... | 2013 | 24007823 |
| expression pattern of glucose metabolism genes in relation to development rate of buffalo (bubalus bubalis) oocytes and in vitro-produced embryos. | the expression pattern of glucose metabolism genes (hexokinase, phosphofructokinase, glucose-6-phosphate dehydrogenase [g6pdh], lactate dehydrogenase [ldh], and pyruvate dehydrogenase [pdh]) were studied in buffalo in vitro-matured oocytes and in vitro-produced embryos cultured under different glucose concentrations (0 mm, 1.5 mm, 5.6 mm, and 10 mm) during in vitro maturation of oocytes and culture of ivf produced embryos. the expression of the genes varied significantly over the cleavage stages ... | 2013 | 23992643 |
| establishment and biological characteristics comparison of chinese swamp buffalo (bubalus bubalis) fibroblast cell lines. | to establish fibroblast cell lines from different tissues and to compare the biological characteristics of those cell lines, five fibroblast cell lines derived from chinese swamp buffalo (bubalus bubalis) were selected for comparative assays. cell style and survival rate (before cryogenic preservation and after recovery) were tested, and karyotype, patterns of isoenzymes of lactic dehydrogenase, malic dehydrogenase, and cell cycle were analyzed. these cell lines had a healthy morphology with a t ... | 2014 | 23990385 |
| oxytocin induced oxidative stress in lactating bubalis bubalis (nili ravi). | oxytocin has been reported for a wide range of adverse effects in different species of lactating animals. the present study was aimed to evaluate the adverse effects of oxytocin on biomarkers of oxidative stress in buffaloes. lactating buffaloes (n = 40) were randomly selected from a commercial dairy farm located in the peri-urban area of district faisalabad, pakistan and divided into two equal groups viz. treatment and control groups, each containing 20 buffaloes. buffaloes in treatment group w ... | 2013 | 23981231 |
| assessment of chromium content of feedstuffs, their estimated requirement, and effects of dietary chromium supplementation on nutrient utilization, growth performance, and mineral balance in summer-exposed buffalo calves (bubalus bubalis). | this study was conducted to determine the chromium content of different feedstuffs, their estimated requirement, and effect of dietary cr supplementation on nutrient intake, nutrient utilization, growth performance, and mineral balance in buffalo calves during summer season. levels of cr was higher in cultivated fodder, moderate in cakes and cereal grains, while straw, grasses, and non-conventional feeds were poor in cr content. to test the effect of cr supplementation in buffalo calves, 0, 0.5, ... | 2013 | 23963742 |
| myoglobin as marker in meat adulteration: a uplc method for determining the presence of pork meat in raw beef burger. | the identification of meat animal species used in raw burgers is very important with respect to economic and religious considerations. therefore, international supervisory bodies have implemented procedures to control the employed meat species. in this paper we propose myoglobin as a powerful molecular marker to evaluate the presence of non-declared meat addition in raw beef burgers by using ultra-performance liquid chromatography (uplc) for the separation and identification of edible animal spe ... | 2013 | 23870895 |
| molecular genetic diversity and quantitation of methanogen in ruminal fluid of buffalo (bubalus bubalis) fed ration (wheat straw and concentrate mixture diet). | high roughage diet causes more methane emissions; however, the total methanogen abundance is not influenced by roughage proportion. technologies to reduce methane emissions are lacking, and development of inhibitors and vaccines that mitigate rumen-derived methane by targeting methanogens relies on present knowledge of the methanogens. in this work, we have investigated molecular diversity of rumen methanogens of surti buffalo. dna from rumen fluid was extracted, and 16s rrna encoding genes were ... | 2013 | 23862067 |
| novel polymorphisms of the igf1r gene and their association with average daily gain in egyptian buffalo (bubalus bubalis). | the objective of this study was to detect insulin-like growth factor 1 receptor (igf1r) polymorphisms, their allele, and genotype frequencies and to determine associations between these polymorphisms and growth traits in egyptian water buffalo. three loci of the igf1r coding region were amplified by rt-pcr and, subsequently, subjected to sequence analysis, followed by single-strand conformation polymorphism to identify different allelic patterns. a total of 11 novel polymorphisms were detected; ... | 2013 | 23820243 |
| molecular cloning, sequencing and structural studies of granulocyte-macrophage colony-stimulating factor (gm-csf) from indian water buffalo (bubalus bubalis). | granulocyte-macrophage colony-stimulating factor (gm-csf) is a cytokine that is essential for growth and development of progenitors of granulocytes and monocytes/macrophages. in this study, we report molecular cloning, sequencing and characterization of gm-csf from indian water buffalo, bubalus bubalis. in addition, we performed sequence and structural analysis for buffalo gm-csf. buffalo gm-csf has been compared with 17 mammalian gm-csfs using multiple sequence alignment and phylogenetic tree. ... | 2014 | 23800159 |
| a signature protein-based method to distinguish mediterranean water buffalo and foreign breed milk. | a novel genetic variant at the αs1-casein locus of water buffalo (wb), 8-residue shorter than its wild-type has been found and sequenced. the internal deletion of the peptide e(35)kvnelst(42) was confirmed by the isolation of the junction peptide. the 8-residue deletion mutant has a molecular weight that is 919 da less than that of the wild-type. the novel isoform with a unique f35-42 deletion could be the result of the skipping of exon 6, generating an exon 6-deleted variant of αs1-casein. the ... | 2013 | 23768399 |
| osteopontin improves sperm capacitation and in vitro fertilization efficiency in buffalo (bubalus bubalis). | the aim of this study was to evaluate the effect of osteopontin (opn), an ubiquitous acid glycoprotein, on in vitro sperm capacitation and on in vitro embryo production (ivep) efficiency in buffalo. in experiment 1, after swim-up separation the sperm were incubated in tyrode albumin lactate pyruvate medium in the absence of capacitating agents (control), with the standard concentration of heparin (0.01 mm) and three different concentrations of opn (0.1, 1, and 10 mcg/ml), both in the presence an ... | 2013 | 23726297 |
| differential expression dynamics of growth differentiation factor9 (gdf9) and bone morphogenetic factor15 (bmp15) mrna transcripts during in vitro maturation of buffalo (bubalus bubalis) cumulus-oocyte complexes. | the present study has evaluated the association of growth differentiation factor9 (gdf9) and bone morphogenetic protein15 (bmp15) mrna expression in cumulus-oocyte complexes (cocs) of buffalo ovary during in vitro maturation (ivm). gdf9 and bmp15 are expressed specifically in mammalian oocytes and also participate in cumulus-oocyte crosstalk. quantitative real-time polymerase chain reaction (qrt-pcr) technique was applied to investigate the relative abundance (ra) of gdf9 and bmp15 mrna transcri ... | 2013 | 23724366 |
| clinicophysiological and haemodynamic effects of fentanyl with xylazine, medetomidine and dexmedetomidine in isoflurane-anaesthetised water buffaloes (bubalus bubalis). | the present study was undertaken to investigate the sedative, analgesic and clinical effects of xylazine, medetomidine and dexmedetomidine with fentanyl as pre-anaesthetics in water buffaloes and to compare the dose-sparing effect of xylazine, medetomidine and dexmedetomidine on thiopental for induction and isoflurane for maintenance of anaesthesia in water buffaloes. six male water buffaloes randomly received intravenous fentanyl (5.0 µg/kg body weight) and xylazine (0.05 mg/kg body weight), fe ... | 2013 | 23718913 |
| pharmacokinetics, urinary excretion and plasma protein binding of ofloxacin in water buffalo calves (bubalus bubalis). | pharmacokinetics and urinary excretion of an intravenous dose of 5 mg.kg-1 ofloxacin were investigated in water buffalo calves. plasma concentrations of ofloxacin were determined by high-performance liquid chromatography. ofloxacin was rapidly distributed from the central to the peripheral compartment as evidenced by a short distribution half-life (0.09 h ± 0.003 h) and high k12 (4.7 h(-1) ± 0.1 h(-1)), and was detected in plasma for 8 h. the large volume of distribution (2.48 l.kg(-1) ± 0.18 l. ... | 2013 | 23718538 |
| effects of milk feeding, frequency and concentration on weaning and buffalo (bubalus bubalis) calf growth, health and behaviour. | growth, weight at birth and daily weight gain (dwg) on 12 water buffalo calves, starting from 6 days of age until completion of weaning, was investigated in this study. different feeding regimens were given to two groups of animals with regard to daily milk replacer: (1) group 1 (g1) received a double concentration in single administration; whereas (2) group 2 (g2) received the same amount of milk replacer split twice daily. blood samples were collected from each calf on days 6, 30, 60 and 90 to ... | 2013 | 23712396 |
| influence of weaning regimen on intake, growth characteristics and plasma blood metabolites in male buffalo calves. | experiment was conducted to evaluate the effect of weaning age on growth performance, feed intake, feed efficiency (fe) and blood metabolites in nili-ravi male buffalo (bubalus bubalis) calves. twenty-four male buffalo calves were assigned to one of the three treatment groups: continuous milk feeding (cmf), limited milk feeding (lmf) and early weaning (ew), and weaned off milk at 12, 10 and 8 weeks of age, respectively. for the first 3 days after birth, calves in all three treatments were fed co ... | 2013 | 23688620 |
| wnt3a signalling pathway in buffalo (bubalus bubalis) embryonic stem cells. | the aim of this study was to investigate the transcriptional profile and role of wnt3a signalling in maintaining buffalo embryonic stem (es) cells in a pluripotent state and in the induction of their differentiation. es cells were derived from embryos produced by in vitro fertilisation (iesc), parthenogenesis (pesc) and hand-made cloning (cesc). the expression of wnt3a, its receptors and intermediate signalling pathways were found to be conserved in es cells derived from the three different sour ... | 2014 | 23656691 |
| comparative evaluation of oral and dermal cypermethrin exposure on antioxidant profile in bubalus bubalis. | cypermethrin, a type ii synthetic pyrethroid insecticide, @ 0.5mg/kg/day for 14 consecutive weeks produced mild signs of toxicity in buffalo calves. significant changes were observed in various antioxidant parameters in blood. there was a marked increase in the extent of lipid peroxidation (33.9%) and enzymic activity of glutathione peroxidase (6.7%), superoxide dismutase (35.0%), catalase (43.7%), glutathione-s-transferase (64.4%), glutathione reductase (36.7%) and glucose-6-phosphate dehydroge ... | 2013 | 23623842 |
| effect of physiologically relevant heat shock on development, apoptosis and expression of some genes in buffalo (bubalus bubalis) embryos produced in vitro. | for investigating the effects of physiologically relevant heat shock, buffalo oocytes/embryos were cultured at 38.5°c (control) or were exposed to 39.5°c (group ii) or 40.5°c (group iii) for 2 h once every day throughout in vitro maturation (ivm), fertilization (ivf) and culture (ivc). percentage of oocytes that developed to 8-cell, 16-cell or blastocyst stage was lower (p < 0.05) and the number of apoptotic nuclei was higher (p < 0.05) for group iii > group ii > controls. at both 8-16-cell and ... | 2013 | 23581430 |
| effect of different thawing rates on post-thaw viability, kinematic parameters and chromatin structure of buffalo (bubalus bubalis) spermatozoa. | the aim of the present study was to evaluate three thawing rates on the post thaw motility, viability and chromatin structure of buffalo semen frozen in 0.5-ml straws. | 2013 | 23577311 |
| proteomic profiles of the embryonic chorioamnion and uterine caruncles in buffaloes (bubalus bubalis) with normal and retarded embryonic development. | the aim of this study was to compare the proteome profiles of the chorioamnion and corresponding caruncle for buffalo embryos that had either normal or retarded development on day 25 after artificial insemination (ai). in experiment 1, embryos that were to subsequently undergo late embryonic mortality had a smaller width on day 25 after ai than embryos associated with pregnancy on day 45 after ai. in experiment 2, 25 italian mediterranean buffaloes underwent transrectal ultrasonography on day 25 ... | 2013 | 23575152 |
| faecal chemical cues in water buffalo that facilitate estrus detection. | chemo-signals are among the reliable non-invasive methods for estrus detection in mammals. water buffalo is a silent heat animal and, hence, there is search for chemo-signals which would be effective non-invasive indicators of estrus state. we analyzed the faecal chemical cues during the estrous cycle in buffalo and to find the estrus-specific faecal volatile compounds adopting bull behavior assay. the faecal samples were collected at three phases of the estrous cycle (i.e., proestrus, estrus an ... | 2013 | 23570909 |
| expression and localization of vascular endothelial growth factor and its receptors in the corpus luteum during oestrous cycle in water buffaloes (bubalus bubalis). | the aim of this study was to document the expression and localization of vegf system comprising of vegf isoforms (vegf 120, vegf 164 and vegf 188) and their receptors (vegfr1 and vegfr2) in buffalo corpus luteum (cl) obtained from different stages of the oestrous cycle. real-time rt-pcr (qpcr), western blot and immunohistochemistry were applied to investigate mrna expression, protein expression and localization of examined factors. in general, all the components of vegf system (the vegf isoforms ... | 2013 | 23551326 |
| isolation of arcobacter species in water buffaloes (bubalus bubalis). | this is the first report of arcobacter spp. in rectal fecal samples from healthy water buffaloes (bubalus bubalis) reared on a dairy farm. arcobacter species were isolated after enrichment, and isolates were identified at species level by multiplex-polymerase chain reaction assay. thirty samples were examined and arcobacter spp. were isolated from 96.7% of water buffaloes tested: 38 arcobacter spp. isolates were obtained, with a. cryaerophilus as the dominant species followed by a. butzleri and ... | 2013 | 23536981 |
| polymorphisms in the ghrelin gene and their associations with milk yield and quality in water buffaloes. | ghrelin is a gastrointestinal hormone that acts in releasing growth hormone and influences the body general metabolism. it has been proposed as a candidate gene for traits such as growth, carcass quality, and milk production of livestock because it influences feed intake. in this context, the aim of this study was to verify the existence of polymorphisms in the ghrelin gene and their associations with milk, fat and protein yield, and percentage in water buffaloes (bubalus bubalis). a group of 24 ... | 2013 | 23497998 |
| cultural legacies, fire ecology, and environmental change in the stone country of arnhem land and kakadu national park, australia. | we use the fire ecology and biogeographical patterns of callitris intratropica, a fire-sensitive conifer, and the asian water buffalo (bubalus bubalis), an introduced mega-herbivore, to examine the hypothesis that the continuation of aboriginal burning and cultural integration of buffalo contribute to greater savanna heterogeneity and diversity in central arnhem land (cal) than kakadu national park (knp). the 'stone country' of the arnhem plateau, extending from knp to cal, is a globally renowne ... | 2013 | 23467505 |
| analysis and functional annotation of expressed sequence tags of water buffalo. | an elucidated genome of domestic livestock river buffalo will contribute enormously to economy and better understanding of genome evolution as well. an attempt is made to obtain genomic information on buffalo, based on total expressed sequence tags (ests) of bubalus bubalis available in public domain. these ests were annotated and classified into 15 different functional categories based on their homology to the known proteins. interestingly, 41.79% of the contigs were found to be buffalo specifi ... | 2013 | 23394367 |
| amount of mrna and localization of vascular endothelial growth factor and its receptors in the ovarian follicle during estrous cycle of water buffalo (bubalus bubalis). | the objective of the present study was to characterize the temporal patterns of gene expression for vascular endothelial growth factors (vegf) and vegf receptors during ovarian follicular growth, development and maturation in buffalo (bubalus bubalis). follicles were classified into four groups according to size and the concentration of estradiol-17β (e2) in follicular fluid (ff): group i (small), 4-6mm diameter, e2>0.5ng/ml of ff; group ii (medium), 7-9mm, e2=0.5-5ng/ml; group iii (large), 10-1 ... | 2013 | 23375984 |
| identification of suitable housekeeping genes for normalization of quantitative real-time pcr data during different physiological stages of mammary gland in riverine buffaloes (bubalus bubalis). | gene expression analysis unravels the complex changes or relations at transcriptomic level. to nullify all type of errors that can be incorporated during any stage of rna extraction into cdna synthesis and for reliable results, the data obtained from qpcr have to be normalized using the appropriate/suitable housekeeping genes (hkgs). unfortunately, till date, no such hkg has been reported for bubaline mammary gland. the objective of the present study was thus to identify and validate the potenti ... | 2013 | 23363300 |
| clinical and molecular study of a new form of hereditary myotonia in murrah water buffalo. | hereditary myotonia caused by mutations in clcn1 has been previously described in humans, goats, dogs, mice and horses. the goal of this study was to characterize the clinical, morphological and genetic features of hereditary myotonia in murrah buffalo. clinical and laboratory evaluations were performed on affected and normal animals. clcn1 cdna and the relevant genomic region from normal and affected animals were sequenced. the affected animals exhibited muscle hypertrophy and stiffness. myoton ... | 2013 | 23339992 |
| effect of tgf-β1 superfamily members on survival of buffalo (bubalus bubalis) embryonic stem-like cells. | this study examined the effects of supplementation of es-like cell culture medium with bone morphogenetic protein (bmp)-4 (0, 10, 20 or 100 ng/ml) or noggin (250, 500 or 750 ng/ml) or tgf-β1 (0, 0.1, 1 or 10 ng/ml) or sb431542 (0, 10, 25 or 50 μm), an inhibitor of tgf-β1 signalling, on survival, colony area and expression level of pluripotency genes in buffalo es-like cells at passage 40-80, under different culture conditions. bmp-4 supplementation significantly reduced (p < 0.05) colony surviva ... | 2013 | 23320894 |
| effect of the stage of estrous cycle on follicular population, oocyte yield and quality, and biochemical composition of serum and follicular fluid in anatolian water buffalo. | this study was designed to investigate the effects that the different stages of the estrous cycle had on the number of surface ovarian follicles and oocyte yield and quality of anatolian water buffalo during peak breeding season. assessments were made on the basis of ovarian morphology, serum and follicular fluid concentrations of variety of biochemical parameters. following slaughter, blood samples were collected from each animal. the stage of estrous cycle was classified as either the luteal o ... | 2013 | 23317849 |
| cryopreservation of zona-free cloned buffalo (bubalus bubalis) embryos: slow freezing vs open-pulled straw vitrification. | this study was carried out to compare the post-thaw cryosurvival rate and the level of apoptosis in vitro produced zona-free cloned buffalo blastocysts subjected to slow freezing or vitrification in open-pulled straws (ops). zona-free cloned embryos produced by handmade cloning were divided into two groups and were cryopreserved either by slow freezing or by vitrification in ops. cryosurvival of blastocysts was determined by their re-expansion rate following post-thaw culture for 22-24 h. the po ... | 2013 | 23281817 |
| traumatic reticuloperitonitis in water buffalo (bubalus bubalis): clinical findings and the associated inflammatory response. | the present study was carried out to describe the clinical picture of traumatic reticuloperitonitis (trp) in water buffalo (bubalus bubalis) and to evaluate the inflammatory and immunologic responses for this clinical condition. twenty-two buffalo with acute local trp were monitored in our study. additionally, 10 clinically healthy buffalo were randomly selected and served as controls. acute local trp was initially diagnosed by clinical examination and confirmed by ultrasonographic (usg) examina ... | 2013 | 26464911 |
| effects of in vitro copper sulphate supplementation on the ejaculated sperm characteristics in water buffaloes (bubalus bubalis). | this study was carried out to investigate effects of copper sulphate (cuso4) additive to semen extenders on sperm parameters: progressive motility, viability, membrane integrity and dna damage, after semen dilution and cryopreservation. semen samples of 5 buffalo bulls of 3-5 years old were collected at 5 different occasions during the autumn 2011. a total number of 25 samples were used in each examination. sperm progressive motility and viability were measured at 0 (t0), 60 (t1) and 120 (t2) mi ... | 2013 | 25593683 |
| the in vitro effect of leptin on semen quality of water buffalo (bubalus bubalis) bulls. | the purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and dna damage). semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (contro ... | 2013 | 25593679 |
| a taxonomic review of the genus megalostomis chevrolat (coleoptera, cryptocephalinae, chrysomelidae). | a taxonomic revision of the genus megalostomis chevrolat, 1836 (coleoptera: chrysomelidae: cryptocephalinae: clytrini: megalostomina) is provided, including new data on geographic ranges. two new species and 34 new synonymies are proposed within the genus megalostomis, leaving 42 valid species in the genus. a checklist of the species of megalostomis is provided, with information on host plants, ant associations, and natural enemies. the study includes a key to all species, diagnoses, description ... | 2013 | 25113596 |
| differential actions of proteinases and neuraminidase on mammalian erythrocyte surface and its impact on erythrocyte agglutination by concanavalin a. | action of proteinases viz. trypsin and chymotrypsin, and neuraminidase on intact erythrocyte membrane proteins and glycophorins (sialoglycoproteins) exposed to cell surface and its impact on lectin (concanavalin a)-mediated agglutination were studied in homo sapiens (human), capra aegagrus hircus (goat) and bubalus bubalis (buffalo). membrane proteins and glycophorins analysis by sds-page as visualized by coomassie brilliant blue and periodic acid-schiff stains, respectively, and agglutination b ... | 2012 | 23255673 |
| impact of buserelin acetate or hcg administration on day 12 post-ovulation on subsequent luteal profile and conception rate in buffalo (bubalus bubalis). | the present study investigated the impact of gonadotropic hormone administration on day 12 post-ovulation on subsequent luteal profile and conception rate in buffaloes. all the buffaloes (n=48) were estrus synchronized by a synthetic analogue of prostaglandin f(2α) (pgf(2α)), administered 11 days apart, followed by insemination during mid to late estrus. to examine the effect of mid-luteal phase hormonal treatment, buffaloes were randomly divided into control (normal saline, n=14), d12-ba (buser ... | 2013 | 23201300 |
| expression pattern of pluripotent markers in different embryonic developmental stages of buffalo (bubalus bubalis) embryos and putative embryonic stem cells generated by parthenogenetic activation. | in this study, we describe the production of buffalo parthenogenetic blastocysts and subsequent isolation of parthenogenetic embryonic stem cell (pgesc)-like cells. pgesc colonies exhibited dome-shaped morphology and were clearly distinguishable from the feeder layer cells. different stages of development of parthenogenetic embryos and derived embryonic stem cell (esc)-like cells expressed key esc-specific markers, including oct-4, nanog, sox-2, foxd3, rex-1, stat-3, telomerase, nucleostemin, an ... | 2012 | 23194456 |
| isolation, characterization, and egfp expression in the buffalo (bubalus bubalis) mammary gland epithelial cell line. | this study was aimed to establish a buffalo mammary epithelial cells (bumecs) line and maintain it for long-term by subculturing. bumecs isolated from lactating buffalo mammary glands were cultured on a collagen matrix gel. bumecs expressed significant amounts of the epithelial cell specific marker cytokeratin 18 as determined by immunohistochemistry. the bumecs displayed monolayer, cobble-stone morphology, and formed lumen-, dome-, and duct-like structures. furthermore, they were capable of syn ... | 2013 | 23180034 |
| effects of glial cell line-derived neurotrophic factor, fibroblast growth factor 2 and epidermal growth factor on proliferation and the expression of some genes in buffalo (bubalus bubalis) spermatogonial cells. | the present study evaluated the effects of glial cell line-derived neurotrophic factor (gdnf), fibroblast growth factor (fgf) 2 and epidermal growth factor (egf) on proliferation and the expression of some genes in spermatogonial cells. spermatogonial cells were isolated from prepubertal buffalo testes and enriched by double enzyme treatment, filtration through 80- and 60-μm nylon mesh filters, differential plating on lectin-coated dishes and percoll density gradient centrifugation. cells were t ... | 2013 | 23171731 |
| glutathione-supplemented tris-citric acid extender improves the post-thaw quality and in vivo fertility of buffalo (bubalus bubalis) bull spermatozoa. | in this study we evaluated the effects of semen extender supplementation with different concentrations of glutathione (gsh) on buffalo (bubalus bubalis) bull sperm motility, plasma membrane integrity, viability and dna integrity as well as in vivo fertility. semen from three nili-ravi buffalo bulls was collected, and qualified semen ejaculates (n=18) were split into five aliquots for dilution (37°c; 50×10(6)spermatozoaml(-1)) with experimental tris-citric acid extender containing 0, 0.5, 1.0, 1. ... | 2012 | 23153697 |
| in vitro culture and morphological characterization of prepubertal buffalo (bubalus bubalis) putative spermatogonial stem cell. | spermatogonial stem cells (sscs) have the unique ability both to self-renew and to produce progeny that undergo differentiation to spermatozoa. the present study has been carried out to develop a method to purify and enrich the pure populations of spermatogonial stem cell like cells in buffalo. | 2012 | 23151879 |
| endometritis impairs luteal development, function, and nitric oxide and ascorbic acid concentrations in buffalo (bubalus bubalis). | a vast majority of the world buffalo resource is concentrated in tropical and subtropical countries. apart from heat stress and poor nutritional availability, endometritis is one of the most commonly encountered reproductive problems limiting fertility and consequently productive potential of the species. as demonstrated recently, endometritis impairs growth and follicular fluid composition of the largest follicle in buffalo. in the present study, the effect of endometritis on luteal development ... | 2013 | 23070685 |
| ticks on buffaloes (bubalus bubalis) in the state of rio de janeiro, brazil. | some tick species have been described parasitizing buffaloes, but reports from brazil are rare. this study aimed to ascertain the species of ticks that parasitize buffaloes in the state of rio de janeiro. seventeen farm properties were visited between june 2008 and december 2009, and buffaloes were inspected in relation to infestation. the ticks were identified and a semi-structured questionnaire on parasitism and treatment against ticks was filled out through interviews with the owners. the spe ... | 2012 | 23070448 |
| toxicity of titanium oxide nanoparticles causes functionality and dna damage in buffalo (bubalus bubalis) sperm in vitro. | the present study has examined the effect of different concentrations (1 μg/ml, 10 μg/ml and 100 μg/ml) of titanium oxide (tio2) nanoparticles (nps) (<100 nm) on viability, membrane integrity, capacitation status and dna integrity of buffalo spermatozoa. characterization of nps was done by the transmission electron microscopy (tem) and dynamic light scattering (dls). sperm chromatin dispersion (scd) test and acridine orange test (aot) were employed to detect dna fragmentation in sperm treated wi ... | 2014 | 23064765 |
| h19 gene methylation study in indian buffalo (bubalus bubalis). | the methylation of dna at cytosine residues within cpg dinucleotides is associated with transcriptional repression and is implicated in maintaining genomic stability and also the silencing of repetitive elements. these imprinted genes are unique as they are expressed exclusively from one parental allele. the present study was carried out to detect methylation status in h19 gene promoter ctcf iii region in three indian buffalo breeds (jaffarabadi, surti and mehsani) by bisulfite sequencing. methy ... | 2013 | 23054415 |
| effects of in vitro selenium addition to the semen extender on the spermatozoa characteristics before and after freezing in water buffaloes (bubalus bubalis). | the aim of the present study was to investigate the effect of in vitro supplementation of selenium on fresh and frozen spermatozoa quality of buffalo (bubalus bubalis) bulls. five healthy buffalo bulls (5 ejaculates from each bull) were used. each ejaculate was diluted at 37 ˚c with tris-based extender containing 0 (control), 0.5, 1, 2, 4 and 8 µg ml(-1) sodium selenite and the sperm motility and viability were evaluated at 0 (t0) (immediately after dilution), 60 (t1) and 120 (t2) min after dilu ... | 2012 | 25653769 |
| anatomic reference for computed tomography of paranasal sinuses and their communication in the egyptian buffalo (bubalus bubalis). | the purpose of this work was to present an anatomic reference for computed tomography (ct) for the paranasal sinuses of adult buffalo fit the use of anatomists, radiologists, clinicians and veterinary students. ct images with the most closely corresponding cross sections of the head were selected and studied serially in a rostral to caudal progression from the level of the interdental space to the level of the nuchal line. the anatomical features were compared with the dissected heads and skulls ... | 2013 | 22994483 |
| a new translocation t(1p;18) in an italian mediterranean river buffalo (bubalus bubalis, 2n = 50) bull: cytogenetic, fertility and inheritance studies. | in recent years increasing attention has been paid to the cytogenetic control of italian mediterranean river buffalo (bbu) bulls authorized as sires which are registered in the stud book. chromosome abnormalities described in this species are mainly numerical and affecting sex chromosomes. during routine cytogenetic analyses performed on young italian mediterranean river buffalo bulls in the progeny test, 1 animal was found to be carrier of a never before reported translocation t(1p;18) originat ... | 2013 | 22986410 |
| influence of season on corpus luteum structure and function and ai outcome in the italian mediterranean buffalo (bubalus bubalis). | the aim was to ascertain whether relationships between corpus luteum (cl) vascularization, cl function, and pregnancy outcome in ai in buffaloes were consistent across the breeding season and transition period to the nonbreeding season in a mediterranean environment. stage of the estrous cycle in italian mediterranean buffaloes was synchronized using the ovsynch with timed ai program and buffaloes were mated by ai in both the breeding season (n = 131) and transition period (n = 125). detailed in ... | 2012 | 22979961 |
| short communication: csn1s1-csn3 (α(s1)-κ-casein) composite genotypes affect detailed milk protein composition of mediterranean water buffalo. | the aim of the study was to investigate the effect of composite csn1s1-csn3 [α(s1)-κ-casein (cn)] genotype on milk protein composition in mediterranean water buffalo. content of α(s1)-cn, α(s2)-cn, β-cn, γ-cn, κ-cn, glycosylated and unglycosylated κ-cn, α-lactalbumin, and β-lactoglobulin was measured by reversed-phase hplc using 621 individual milk samples. genotypes at csn1s1 and csn3 were also obtained by reversed-phase hplc. two alleles were detected at csn1s1 (corresponding to the a and b va ... | 2012 | 22959943 |
| expression of leptin and its receptor in corpus luteum during estrous cycle in buffalo (bubalus bubalis). | leptin is supposed to play a crucial role in ovarian luteal dynamics. the present study was aimed to investigate the importance of leptin and its receptors in buffalo corpus luteum (cl) obtained from different stages of the estrous cycle. real-time rt-pcr (qpcr), western blot and immunohistochemistry techniques were applied to investigate mrna expression, protein expression and localization of examined factors. additionally to assess the contribution of leptin in progesterone production the expr ... | 2012 | 22959515 |
| rock inhibitor y-27632 enhances the survivability of dissociated buffalo (bubalus bubalis) embryonic stem cell-like cells. | this study investigated the effects of supplementation of culture medium with 10 μm y-27632, a specific inhibitor of rho kinase activity, for 6 days on self-renewal of buffalo embryonic stem (es) cell-like cells at passage 50-80. y-27632 increased mean colony area (p<0.05) although it did not improve their survival. it decreased oct4 expression (p<0.05), increased nanog expression (p<0.05), but had no effect on sox2 expression. it also increased expression of anti-apoptotic gene bcl-2 (p<0.05) a ... | 2013 | 22951036 |
| birth of cloned calves from vitrified-warmed zona-free buffalo (bubalus bubalis) embryos produced by hand-made cloning. | the availability of techniques for the vitrification of cloned blastocysts can improve their effective use. the present study compared the developmental competence of buffalo cloned embryos derived from adult (baf), newborn (bnf) and fetal fibroblast (bff) before and after vitrification. despite similar cleavage rates among the three groups, the blastocyst rate was lower for baf- than bnf- and bff-derived embryos (30.2±2.2% vs 41.7±1.7% and 39.1±2.1%, respectively; p<0.01). the total cell number ... | 2013 | 22950886 |
| "remake" by high-throughput sequencing of the microbiota involved in the production of water buffalo mozzarella cheese. | intermediates of production of two batches of traditional mozzarella cheese were analyzed by culture-independent pyrosequencing. the quantitative distribution of taxa within the samples suggested that thermophilic lactic acid bacteria from the natural starter were mainly responsible for the fermentation, while microorganisms found in raw milk did not develop during fermentation. | 2012 | 22941080 |
| optimization of cryopreservation of buffalo (bubalus bubalis) blastocysts produced by in vitro fertilization and somatic cell nuclear transfer. | the objective of this study was to optimize cryopreservation conditions for buffalo in vitro produced (ivp) embryos. the in vitro fertilized (ivf) and somatic cell nuclear transferred (scnt) blastocysts were vitrified with either 40% ethylene glycol (eg), 25% eg + 25% dimethylsulfoxide (dmso), or 20% eg + 20% dmso + 0.5 m sucrose, and the ivf blastocysts produced from abattoir-derived ovaries were also slow-frozen with either 10% eg or 0.05 m trehalose dehydrate + 1.8% eg + 0.4% bsa. cryosurviva ... | 2012 | 22925650 |
| cloning and sequencing of the rdna gene family of the water buffalo (bubalus bubalis). | the rdna genes coding for ribosomal rna in animals are complicated repeat sequences with high gc content. we amplified water buffalo rdna gene sequences with the long and accurate (la) pcr method, using la taq dna polymerase and gc buffer, based on bioinformatic analysis of related organisms. the rdna genes were found to consist of 9016 nucleotides, including three rrna genes and two internal transcribed spacers (its), which we named 18s rrna, its1, 5.8s rrna, its2 and 28s rrna. we tested and op ... | 2012 | 22869068 |
| immunolocalization, gene expression, and enzymatic activity of cyclooxygenases, prostaglandin e2-9-ketoreductase, and nitric oxide synthases in mediterranean buffalo (bubalus bubalis) corpora lutea during diestrus. | immunopresence, gene expression, and enzymatic activity of cyclooxygenase 1 (cox1), cox2, pge2-9-ketoreductase (pge2-9-k), endothelial (enos), and inducible nitric oxide synthases (inos), and hormone in vitro production were examined in early, mid, late, and regressive buffalo corpora lutea (cl). cox1 immunosignals were detected in the cytoplasm of small luteal cells, cox2 in large luteal cells, and pge2-9-k in all luteal cells. cox2 and pge2-9-k immunosignals were greater in late cl. immunopres ... | 2012 | 22865504 |
| parentage verification in field progeny testing program of mehsana buffalo. | the present study was undertaken to construct a multiplex microsatellite panel for parentage testing in mehsana buffalo (bubalus bubalis). the study was based on a total of 212 mehsana buffalos (100 dams, 100 daughters, and 12 sires). genomic dna was extracted from blood and semen samples. a panel of 10 microsatellite markers (cssm61, ilsts29, ilsts17, ilsts28, cssm57, cssm22, ilsts61, cssm8, eth152, and ilsts11) was amplified in a single multiplex reaction and analyzed by capillary electrophore ... | 2012 | 22851245 |
| effect of ground corn cob replacement for cassava chip on feed intake, rumen fermentation and urinary derivatives in swamp buffaloes. | four thai - rumen fistulated male swamp buffaloes (bubalus bubalis), about four years old with 400±20 kg liveweight, were randomly assigned according to a 4×4 latin square design to receive dietary treatments. the treatments were: ground corn cob (gcc) replacement for cassava chip (cc) in concentrate at 0% (t1); gcc replacement at 33% (t2); gcc replacement at 67% (t3); and gcc replacement at 100% (t4), respectively. during the experiment, concentrate was offered at 0.5% bw while 5% urea-treated ... | 2012 | 25049671 |
| culture, characterization and differentiation of cells from buffalo (bubalus bubalis) amnion. | stem cells present an important tool in livestock assisted reproduction and veterinary therapeutic field such as tissue engineering. we report for the first time isolation of pluripotent stem cell-like cells expressing pluripotency markers (alkaline phospahatase, oct-4, nanog and sox-2) from the amnion of water buffalo (bubalus bubalis). the cells showed no apparent abnormalities in their chromosomal profiles before and after cryopreservation. the cytochemical staining revealed that pluripotent ... | 2013 | 22820992 |
| effect of parity, days in milk, and milk yield on detailed milk protein composition in mediterranean water buffalo. | the effects of some nongenetic factors on milk protein fraction contents and relative proportions were estimated in 606 individual milk samples of mediterranean water buffalo. content of α(s1)-casein (cn), α(s2)-cn, β-cn, γ-cn, κκ-cn, glycosylated κ-cn (glyco-κ-cn), α-lactalbumin, and β-lactoglobulin was measured by reversed-phase hplc. relative contents of α(s1)-cn%, α(s2)-cn%, β-cn%, and κ-cn% were, respectively, 32.1, 17.1, 34.5, and 15.7%, whereas γ-cn% accounted for 0.6% of total casein con ... | 2012 | 22818435 |
| establishment and characterization of a buffalo (bubalus bubalis) mammary epithelial cell line. | the objective of this study was to establish the buffalo mammary epithelial cell line (bumec) and characterize its mammary specific functions. | 2012 | 22792341 |
| buffalo (bubalus bubalis) es cell-like cells are capable of in vitro skeletal myogenic differentiation. | when buffalo embryonic stem (es) cell-like cells that expressed surface markers ssea-4, tra-1-60, tra-1-81, cd9 and cd90 and intracellular markers oct4, sox2 and foxd3, as shown by immunofluorescence, and that expressed rex-1 and nucleostemin as confirmed by rt-pcr, were subjected to suspension culture in hanging drops in absence of lif and buffalo foetal fibroblast feeder layer support, they differentiated to form three-dimensional embryoid bodies (ebs). of 231 ebs examined on day 3 of suspensi ... | 2013 | 22788718 |
| prognosis prediction of uterine torsion mechanical treatment (rolling) after estimation of calcium and creatinine level in the serum of buffaloes (bubalus bubalis). | the present study was carried out to investigate the relationship between creatinine and calcium concentration in buffalo serum in cases of uterine torsion before rolling, and 1 h and 24 h after calving. the degree, duration and site of uterine torsion, as well as fetus viability, time needed for cervical dilation, and the occurrence of uterine rupture were recorded. a total of 150 pregnant buffaloes suffering from colic and anorexia were brought to our clinic and clinically examined. of these, ... | 2012 | 22763076 |
| collagen-iv supported embryoid bodies formation and differentiation from buffalo (bubalus bubalis) embryonic stem cells. | embryoid bodies (ebs) are used as in vitro model to study early extraembryonic tissue formation and differentiation. in this study, a novel method using three dimensional extracellular matrices for in vitro generation of ebs from buffalo embryonic stem (es) cells and its differentiation potential by teratoma formation was successfully established. in vitro derived inner cell masses (icms) of hatched buffalo blastocyst were cultured on buffalo fetal fibroblast feeder layer for primary cell colony ... | 2012 | 22749767 |
| serum concentrations of haptoglobin and serum amyloid a in water buffaloes (bubalus bubalis) with abomasal ulcer. | to evaluate the serum concentrations of haptoglobin (hp) and serum amyloid a (saa) in water buffaloes with abomasal ulcers, the abomasums of 100 randomly selected water buffaloes were examined after slaughter. type i abomasal ulcers were found in 56 out of 100 buffaloes. serum concentrations of hp and saa were measured. there was no significant difference between affected and non-affected buffaloes in the serum concentrations of hp and saa. the serum concentrations of hp and saa had no significa ... | 2012 | 25610571 |