| fate of phase i and phase ii coxiella burnetii in several macrophage-like tumor cell lines. | several macrophage-like tumor cell lines of murine origin were exposed to phase i and phase ii coxiella burnetii, and the subsequent fate of the parasites was determined by electron and bright-field microscopy. phase i c. burnetii proliferated within and established a persistent infection of p388d1, j774, and pu-5-ir cell lines but not of wehi-3 and wehi-274 cell lines. phase ii c. burnetii, however, entered into and persistently infected all five cell lines. the parasites proliferated within va ... | 1981 | 7263063 |
| developmental cycle of coxiella burnetii: structure and morphogenesis of vegetative and sporogenic differentiations. | coxiella burnetii is a gram-variable obligate intracellular bacterium which carries out its development cycle in the phagolysosome of eucaryotic cells. ultrastructural analysis of c. burnetii, in situ and after renografin purification, by transmission electron microscopy of lead-stained thin sections has revealed extreme pleomorphism as demonstrated by two morphological cell types, a large cell variant (lcv) and a small cell variant (scv). potassium permanganate staining of purified rickettsiae ... | 1981 | 7275931 |
| cryptic epidemic of q fever in a medical school. | pregnant sheep used in perinatel research were the source of a large outbreak of q fever in 1980 among the faculty and staff of a medical school. complement-fixation tests with phase ii coxiella burnetii antigen were positive in 81 persons, of whom 58 (71.6%) had q fever during the first eight months of 1980. microagglutination and/or indirect immunofluorescence tests of 485 sera were positive in an additional 56 persons. only 41 of the 137 seropositive persons were involved in caring for or wor ... | 1981 | 7276623 |
| aerosol q fever infection of the nude mouse. | athymic nude mice and euthymic littermate controls were exposed to 10(4) coxiella burnetii organisms by small-particle aerosol. antibody response with and without 2-mercaptoethanol treatment of serum was determined at various intervals after infection and serial kills were done to determine morphologic changes in both mouse phenotypes. total antibody titers determined by the indirect fluorescent antibody technique to phase i and phase ii c. burnetii were identical for both groups of mice. microa ... | 1981 | 7281464 |
| q fever in cyprus: a short review. | | 1981 | 7306776 |
| [q fever (author's transl)]. | comparative analysis of two q fever epidemics in baden-württemberg demonstrated the following characteristics of this zoo-anthroponosis endemic in southern germany: 1. affected areas are situated along sheep tracks and natural concentrations of the dermacentor marginatus. 2. the disease spreads in waves. 3. it causes typical symptoms, particular an intense tension headache, and typical radiographic findings. men are more commonly and more severely affected than women. children are not affected d ... | 1981 | 7307989 |
| [epidemiologic, serologic, clinical and therapeutic aspects of q fever endocarditis: report of two cases (author's transl)]. | | 1981 | 7321652 |
| legionnaires' disease: a review of the epidemiology and clinical manifestations of a newly recognized infection. | during the 2-year period 1977 through 1979, 26 patients with legionnaires' disease were seen at the mayo clinic and affiliated hospitals. the patients ranged in age from 17 to 81 years with a median of 51 years. twelve (46%) were immunologically compromised. most of the other patients had underlying chronic tobacco bronchitis. hectic fever, cough, and diarrhea were common symptoms. chest radiographs showed patchy perihilar infiltrates that often progressed to consolidation. diagnosis was made by ... | 1980 | 7354652 |
| q fever and raw milk. | | 1980 | 7358543 |
| igm globulin response in q fever (coxiella burneti) infections. | the igm globulin response has been measured following the onset of primary q fever infections. a complement fixation test method was used to measure the igm after separation by ultracentrifugation in a sucrose density gradient. specific igm was demonstrable between 2 and 10 wk after onset of symptoms. the test can be used to confirm a recent infection when only a 'convalescent' blood specimen has been collected and will be useful in the assessment of workers' compensation claims. | 1980 | 7432817 |
| [clinico-epidemiological considerations on an outbreak of q fever in the argeş region]. | the present paper reports on a q fever episode, including 45 subjects (caretakers, technicians, workers building stables) in a zootechnical cooperative in the district of argeş. in the new unit milch and reproduction cows were brought from various parts of the district. none of the animals showed visible signs of the disease. no cases of q fever had been reported in man or animals in the locality until then. the episode had an explosive character, the outbreak occurring during the calving season ... | 1980 | 7433815 |
| mechanisms of protective immunogenicity of microbial vaccines: effects of cyclophosphamide pretreatment in venezuelan encephalitis, q fever and tularaemia. | administration of high-dose (250 mg/kg) cyclophosphamide (cy) to guinea-pigs and mice 3 days prior to immunization with inactivated vaccine derived from venezuelan encephalitis virus (ve), coxiella burnetii and francisella tularensis resulted in accentuated and prolonged delayed-type hypersensitivity (dth) and in vitro cellular immunity (cmi) to specific antigen. humoral antibody were either absent or significantly lower in cy-pretreated animals compared to immunized non-pretreated controls. cy ... | 1980 | 7438552 |
| time course of lung function changes in atypical pneumonia. | we measured pulmonary function in each of 21 patients suffering from "atypical", non-bacterial pneumonia during the acute illness and during convalescence (two to 18 months) to study the course and the nature of functional impairment at different stages of the disease. in six patients, no aetiological agent was found. an aetiological agent was identified in 15 of the patients: mycoplasma pneumoniae (seven patients), influenza a (three patients), parainfluenza 3 (one patient), varicella (two pati ... | 1980 | 7444825 |
| [q fever and coxiella burnetii]. | | 1995 | 7474343 |
| [scientific raisins from 125 year smw (swiss medical weekly) q fever (queensland fever) in switzerland (endemic pneumonia caused by rickettsia burneti). 1948]. | | 1995 | 7481660 |
| efficacy of coxiella burnetii and its chloroform-methanol residue (cmr) fraction against rift valley fever virus infection in mice. | strains of coxiella burnetii phase i and ii whole cells (wc-i and wc-ii) or whole cell fractions were assessed for their potential to induce long-lasting protection in endotoxin-non-responder c3h/hej or cd-1 mice against rift valley fever (rvf) virus challenge. among the whole cell fractions, only the chloroform-methanol residue (cmr), administered as a single dose (100 micrograms per mouse) 24 h before viral challenge, effectively protected 100% of the mice from rvf virus; the cmr of the ohio s ... | 1995 | 7486951 |
| the risk of acquiring q fever on farms: a seroepidemiological study. | to determine the occupational risk of q fever. | 1995 | 7489053 |
| seroepidemiology of q fever in nova scotia: evidence for age dependent cohorts and geographical distribution. | to determine the incidence of q fever in nova scotia, a randomly selected sample of 492 volunteers aged 18-70 years was recruited by mail from all 18 urban and rural counties in this province. volunteers were followed from 1988 to 1991 for antibody titres to coxiella burnetii antigens. analysis of seroprevalence by age revealed two statistically different cohorts. those younger than 35 years had a low, stable seroprevalence while those 35 years and older had a seroprevalence statistically correl ... | 1995 | 7489773 |
| q fever serology: cutoff determination for microimmunofluorescence. | q fever, a worldwide zoonosis caused by coxiella burnetii, lacks clinical specificity and may present as acute or chronic disease. because of this polymorphism, serological confirmation is necessary to assess the diagnosis. although microimmunofluorescence is our reference technique, the cutoff titers that are currently used to make a diagnosis of active or chronic q fever were determined years ago with limited series of patients and sera. we determined the titers of immunoglobulin g (igg), igm, ... | 1994 | 7496944 |
| phylogenic homogeneity of coxiella burnetii strains as determinated by 16s ribosomal rna sequencing. | dna coding for the 16s rrna of six strains of the obligate intracellular bacterium coxiella burnetii was directly amplified from lysed host cells using the polymerase chain reaction. the amplification product was sequenced using a linear-pcr procedure and compared with other published 16s rrna sequences. the results of this analysis confirm the position of c. burnetii in the gamma subgroup of the proteobacteria. the data show that all of the c. burnetii strains are highly related (> 99%) on the ... | 1993 | 7505761 |
| immunohistochemical detection of coxiella burnetti in formalin-fixed placenta. | | 1994 | 7516717 |
| the 16s/23s ribosomal spacer region of coxiella burnetti. | the 16s/23s spacer region of coxiella burnetti isolate nine nile, phase 1, was sequenced. sequence analysis revealed two trna coding regions for trna(ile) and trna(ala). dna sequence alignment demonstrated significant homology with trna species from pseudomonas aeruginosa and rhodobacter sphaeroides, respectively. the non-coding trna spacer region was unique to coxiella burnetti, based on database alignment. | 1994 | 7531158 |
| detection of mip-like sequences and mip-related proteins within the family rickettsiaceae. | the mip surface protein, a prokaryotic analog of the fk506-binding proteins, enhances the ability of legionella pneumophila to infect macrophages and protozoa. using mip-specific probes and low-stringency southern hybridizations, we have detected dna sequences homologous to mip within coxiella burnetii and rochalimaea quintana. using specific anti-mip antisera and immunoblot analysis, we also detected mip-related proteins within these bacteria as well as within rickettsia and ehrlichia species. ... | 1995 | 7532505 |
| [the comparative characteristics of the immunogenic and immunomodulating activity of different antigenic preparations of the causative agent of q fever coxiella burnetii]. | the immunogenicity of the coxiella burnetii corpuscle antigen and two its components, obtained after the treatment with chloroform-methanol or extraction by trichloroacetic acid, was investigated. all the studied antigens were highly immunogenic. a strong correlation was discovered between the immunogenic and immunomodulating properties of the c. burnetii antigens. cells of the mononuclear phagocytic system are the targets for stimulating action of the studied antigens. a cyclic increase of anti ... | 1994 | 7535962 |
| differentiation of bartonella-like isolates at the species level by pcr-restriction fragment length polymorphism in the citrate synthase gene. | the citrate synthase gene (glta) of bartonella henselae was cloned and sequenced to compare genetic divergence among alpha and gamma branches of the class proteobacteria and to develop enhanced genotypic reagents for b. henselae identification. b. henselae glta is 1,293 nucleotides in length and 63 to 66% homologous with corresponding gene sequences of rickettsia prowazekii, escherichia coli, and coxiella burnetii. the observed genetic variability suggests that glta sequences can provide a usefu ... | 1995 | 7545181 |
| isolation of coxiella burnetii and of an unknown rickettsial organism from ixodes ricinus ticks collected in austria. | two strains of coxiella burnetii and two strains of an unidentified rickettsial organism were isolated for the first time from ixodes ricinus ticks collected in the alpine region of tirol, austria. the c. burnetii strains belong to the group of agents causing acute forms of q fever. the other two strains of isolated rickettsial agent share some antigenic epitopes with c. burnetii and r. prowazekii but they differ from them by their high sensitivity to freezing and refreezing and by poor multipli ... | 1994 | 7545588 |
| validation of an enzyme immunoassay for serodiagnosis of acute q fever. | an enzyme immunoassay was validated for the serodiagnosis of acute q fever. minimum positive tests were determined for both serial dilutions and a single dilution of patient sera. to establish the specificity of the test, 152 serum samples were tested from individuals with no evidence of past coxiella burnetii infection. diagnostic titers were set at > or = 128 for the igm and igg responses to phase i, at > or = 512 for the igm response to phase ii and at > or = 1,024 for the igg response to pha ... | 1995 | 7556231 |
| roa307, a protein encoded on coxiella burnetii plasmid qph1, shows homology to proteins encoded in the replication origin region of bacterial chromosomes. | tnphoa mutagenesis identified an open reading frame, roa307, immediately upstream of the partition locus qsopab on the coxiella burnetii plasmid qph1. the protein sequence deduced from roa307 displayed homology to orf290 of pseudomonas putida, orf283 and orf282 (spooj) of bacillus subtilis-hypothetical products of genes in the chromosomal replication origin region. expression of roa307 was demonstrated by phoa activity of an roa307-phoa fusion. | 1995 | 7565613 |
| mesangioproliferative glomerulonephritis and antibodies to phospholipids in a patient with acute q fever: case report. | glomerulonephritis is a rare complication of q fever and is usually associated with the chronic form of disease, i.e., infectious endocarditis. we describe a 31-year-old man with a self-limited acute febrile illness due to coxiella burnetii infection who had acute renal failure secondary to mesangioproliferative glomerulonephritis. antibodies to phospholipids were detected and suggested the diagnosis of q fever in this case. | 1995 | 7578731 |
| seroepidemiologic study of three zoonoses (leptospirosis, q fever, and tularemia) among trappers in québec, canada. | this study was undertaken to evaluate the prevalence of antibodies against francisella tularensis, coxiella burnetii, and certain serovars of leptospira interrogans among trappers in québec, canada. muskrat trapping was identified as a risk factor for f. tularensis infection, whereas having a cat at home apparently protected trappers against infection by l. interrogans. high percentages of control sera were positive for antibodies against c. burnetii (15%) and l. interrogans (5%), most frequentl ... | 1995 | 7583933 |
| virulence-associated fyua/irp2 gene cluster of yersinia enterocolitica biotype 1b carries a novel insertion sequence is1328. | the fyua/irp2 gene cluster, which is part of the yersinia pestis pigmentation (pgm) locus encoding genes involved in iron uptake and virulence, is present in all pesticin-sensitive bacteria. in y. enterocolitica biotype 1b strains (serotypes o8, o20, o21), the fyua/irp2 gene cluster carries an insertion of a novel repetitive sequence, is1328. it was also found in the genome of y. enterocolitica o5 (biotype 1a) and o13 (biotype 1b), but not in pesticin-sensitive y. pseudotuberculosis o1 and esche ... | 1995 | 7607411 |
| q fever in the greek island of crete: epidemiologic, clinical, and therapeutic data from 98 cases. | a retrospective study was undertaken in crete, greece, to investigate the epidemiologic, clinical, and therapeutic aspects of q fever. over a period of 5 years (1989-1993), 1,298 patients were examined and 98 cases were identified. individuals who were aged 20-29 years and 30-39 years appeared to have an increased risk of infection. contact with animals was found to be a major risk factor for acquisition of q fever. the predominant clinical manifestations of the infection were fever (91.7% of pa ... | 1995 | 7620016 |
| acute q fever hepatitis in taiwan. | we report a case of a chronic hepatitis b carrier with an episode of acute hepatitis. the patient presented with a headache, arthralgias, jaundice and fever. while the laboratory tests mimicked chronic hepatitis b with an acute exacerbation, lipogranulomatous changes seen in the liver biopsy strongly suggested the presence of q fever. serology testing for coxiella burneti proved positive and the patient responded to tetracycline therapy. while previously unreported in taiwan, q fever is importan ... | 1995 | 7620101 |
| the partition region of plasmid qph1 is a member of a family of two trans-acting factors as implied by sequence analysis. | sequencing analysis revealed that the partition region of the coxiella burnetii plasmid qph1 contains a putative operon, designated qsopab. the two open reading frames (orfs), qsopa and qsopb, specify the qsopa and qsopb proteins, with deduced molecular masses of 45.7 and 37.6 kda, respectively. maxicell analysis demonstrated that although qsopb was located downstream from qsopa, it had its own promoter that was active in escherichia coli. several direct or inverted repeats were found around thi ... | 1995 | 7628719 |
| [changed etiopathogenic and clinical features of infective endocarditis]. | the authors report their experience on etiological and clinical aspects of infective endocarditis (ie). a series of 182 consecutive patients, including 83 cases of medical ie, 73 cases of ie in intravenous drug abusers (da), 22 cases of ie on late prosthetic valves and 4 cases of ie on early prosthetic valves were evaluated since 1976. medical ie occurred frequently in the elderly patients and in most of the cases (80%) involved natural valves with underlying abnormalities, either rheumatic (42% ... | 1994 | 7634278 |
| analysis of a coxiella burnetti gene product that activates capsule synthesis in escherichia coli: requirement for the heat shock chaperone dnak and the two-component regulator rcsc. | a 1.2-kb ecori genomic dna fragment of coxiella burnetti, when cloned onto a multicopy plasmid, was found to induce capsule synthesis (mucoidy) in escherichia coli. nucleotide sequence analysis revealed the presence of an open reading frame that could encode a protein of 270 amino acids. insertion of a tet cassette into a unique nrui restriction site resulted in the loss of induction of mucoidy. because of its ability to induce mucoidy, we designated this gene mucz. computer search for homologie ... | 1995 | 7635811 |
| q-fever pneumonia in the negev region of israel: a review of 20 patients hospitalised over a period of one year. | three-hundred and forty-six patients with community acquired pneumonia were included in a prospective study of patients hospitalised over a 12-month period in the soroka medical center in beer-sheva, israel. q-fever pneumonia (qfp) was diagnosed in 20 patients (5.8%). a detailed epidemiological and clinical description of this disease, is presented. | 1995 | 7636279 |
| entry and survival of leishmania amazonensis amastigotes within phagolysosome-like vacuoles that shelter coxiella burnetii in chinese hamster ovary cells. | coxiella burnetii, a rickettsia, and leishmania amazonensis, a protozoan flagellate, lodge in their host cells within large phagolysosome-like vacuoles. in the present study, c. burnetii-infected vero or cho cells were superinfected with l. amazonensis amastigotes to determine if these parasites can home to and survive within heterologous vacuoles. six hours after superinfection, leishmania amastigotes were located almost exclusively within large coxiella-containing vacuoles. thereafter, the num ... | 1995 | 7642284 |
| [coxiella burnetii endocarditis on a mechanical valvular prosthesis. apropos of 2 cases]. | the authors report two cases of prosthetic valve endocarditis due to coxiella burnetii. the histories were chronic and complex suggesting an auto-immune disease: prolonged recurrent fever despite antibiotic therapy with a biological inflammatory syndrome whilst blood cultures remained negative. the first patient presented with prosthetic valve dehiscence and acute glomerulonephritis. the second patient had coagulation defects with prosthetic valve thrombosis, mesenteric adenopathy and congestive ... | 1995 | 7646271 |
| antibodies against rickettsiaceae in dogs of setúbal, portugal. | an indirect fluorescent antibody test was performed on sera collected from dogs housed in the municipal kennel of setúbal to assess the prevalence of antibodies to ehrlichia canis, the causative agent of canine ehrlichiosis and to rickettsia conorii, agent of boutonneuse fever in humans. two other members of the family rickettsiaceae, coxiella burnetii and rickettsia typhi, were included in the serosurvey. of the 104 dogs tested, 85.6% had antibodies to r. conorii, 50% to e. canis, 26.9% to r. t ... | 1995 | 7655402 |
| seroepidemiological survey of rickettsial infections among blood donors in central tunisia. | in this report we attempt to evaluate the prevalence of antibodies against rickettsia conorii, r. typhi, coxiella burnettii, and ehrlichia chaffeensis in central tunisia. five hundred sera from blood donors, collected between march and june 1993, were tested for these 4 antibodies using an indirect immunofluorescence antibody assay (ifa). nine percent of the sera had antibodies against r. conorii (igg > 1:32) by ifa, and 8% by western blotting; with ifa, 3.6% had antibodies to r. typhi, 26% to c ... | 1995 | 7660428 |
| [the immune response in those ill with and convalescing from q fever studied by using immunoenzyme analysis]. | the study of immune response in q-fever patients and convalescents at different periods from the beginning of the disease demonstrated the diagnostic possibilities of the complement fixation test (cft), the microagglutination test (mat), the passive immunofluorescence test (pift) and the enzyme immunoassay (eia). high effectiveness of eia in comparison with cft, mat and pift was noted (97, 63, 87 and 91% respectively). the sensitivity of eia was not less than 100-250 times higher than that of cf ... | 1995 | 7660714 |
| osteoarticular infection due to coxiella burnetii in children. | we report three cases of chronic osteoarticular infections associated with q fever in children. in two of them, serologic diagnosis was confirmed by direct immunofluorescence of coxiella burnetii in the synovial and bone biopsy specimen. we suspect that bone infections due to coxiella burnetii could be underdiagnosed because this etiology is not frequently seen. | 1995 | 7670995 |
| q fever endocarditis. | q fever is a widespread disease caused by the rickettsia coxiella burnetii, an obligate intracellular bacteria which man usually acquires through the inhalation of infected dust from subclinically infected animals. q fever may be acute or chronic. the chronic form mostly presents as endocarditis, which is difficult to diagnose and may ultimately be fatal. immunocompromised conditions and underlying heart disease are the most important risk factors to consider in cases of q fever endocarditis. th ... | 1995 | 7671918 |
| eia with species-specific monoclonal antibodies: a novel seroepidemiologic tool for determination of the etiologic agent of spotted fever rickettsiosis. | all currently available assays for antibodies to the etiologic agents of spotted fever group rickettsioses detect reactivity with antigens of lipopolysaccharides and the major cell wall proteins, which contain epitopes that are shared among many spotted fever group rickettsiae. the hypothesis of this study was that a monoclonal antibody to a species-specific epitope of rickettsia rickettsii would be blocked from binding to the rickettsial surface if the rickettsiae were incubated previously with ... | 1993 | 7693827 |
| latent q fever in cattle in southern moravia (czech republic). | in 1991-92, a complement fixation test technique (titre > or = 8) was used in serological assays of antibodies to coxiella burnetti performed in cattle in southern moravia. antibodies were ascertained in 6.1% to 213 aborting cows. the cows with a positive finding after abortion came from 13 different farms. on two of the farms (h. v. and p.), all the cattle were tested. on the h. v. farm, antibodies were ascertained in 4.2% of 120 cows, in 1 of 36 calves and in none of 93 heifers. on the p. farm ... | 1994 | 7697029 |
| characterization of the succinate dehydrogenase-encoding gene cluster (sdh) from the rickettsia coxiella burnetii. | we have identified and sequenced four genes that encode the protein subunits comprising the succinate dehydrogenase enzyme complex (sdh) of the rickettsia coxiella burnetii. the sdh-encoding gene cluster (sdhcdab) begins 3326 bp upstream from the citrate synthase-encoding gene (glta) start codon and is read with opposite polarity. an open reading frame encoding the n-terminal 280 amino acids (aa) of 2-oxoglutarate dehydrogenase (suca) begins 24 bp downstream from the stop codon of the gene speci ... | 1995 | 7698664 |
| fusion between large phagocytic vesicles: targeting of yeast and other particulates to phagolysosomes that shelter the bacterium coxiella burnetii or the protozoan leishmania amazonensis in chinese hamster ovary cells. | this report examines the fusion of phagocytic vesicles with the large phagolysosome-like vacuoles induced in chinese hamster ovary cells by the bacterium coxiella burnetti or the protozoan flagellate leishmania amazonensis. infection by these organisms is compatible with cell survival and multiplication. fusion was inferred from the transfer of microscopically identifiable particles from donor to target vesicles. donor vesicles contained heat-killed yeast, zymosan, beta-glucan or latex beads tak ... | 1994 | 7699006 |
| secretion of proteins by coxiella burnetii. | viable coxiella burnetii organisms were isolated from the culture medium of persistently infected baby hamster kidney (bhk-21) fibroblasts. when these organisms were incubated in host-cell-free medium at low ph, some of the de novo-synthesized protein made by the bacteria was translocated to the exterior of the cell. the exported protein was detectable after 2-7 h incubation at 37 degrees c. no evidence was found to suggest that protein accumulation in the medium was due to leakiness caused by c ... | 1995 | 7704266 |
| isolation of coxiella burnetii from heart valves of patients treated for q fever endocarditis. | coxiella burnetii was isolated from the valve material of two patients who underwent valvectomy because of progressive congestive heart failure due to endocarditis. in each case antibiotic therapy was administered for several months prior to valvectomy. classical histopathological examination of the valves did not reveal an etiology. however, coxiella-like organisms were demonstrated in valvular material with köster, stamp, and giemsa stains, and the organisms were grown in cell culture. antibod ... | 1995 | 7714203 |
| atypical pneumonias in children. | the major agents responsible for atypical pneumonia in children include a wide variety of organisms, one mycoplasma species, two chlamydia species, a rickettsia, and one fastidious bacterium. mycoplasma pneumoniae and c. pneumoniae together may be responsible for over 40% of these infections. recognition of the role that these agents play in pneumonia is important since many of the diagnostic methods used to detect these organisms are not available in most hospital microbiology laboratories. if ... | 1995 | 7718203 |
| [follow-up studies of the detection of igg1 antibodies to coxiella burnetii in blood and milk from cattle in relation to the reproductive cycle and milk production]. | during a 14 month period, 36 dairy cattle were selected from a herd (n = 103), after igg1 antibodies to coxiella burnetii had repeatedly been detected in blood and milk, as measured by elisa. elisa results were analyzed with reference to reproductive cycle, daily milk yield, as well as daily concentration, and output of milk protein. the rate of positive blood samples slightly grew from 47.6% in the non-pregnant period, and 46.8% in the first half of pregnancy to 52.0% in the second half of preg ... | 1994 | 7720550 |
| cross-species complementation of the indispensable escherichia coli era gene highlights amino acid regions essential for activity. | era is an essential gtp binding protein in escherichia coli. two homologs of this protein, sgp from streptococcus mutans and era from coxiella burnetii, can substitute for the essential function of era in e. coli. site-specific and randomly generated era mutants which may indicate regions of the protein that are of functional importance are described. | 1995 | 7721709 |
| seroepidemiological survey of coxiella burnetii in domestic cats in japan. | cats are assumed to be one of the most important reservoirs of causative agent of human q fever especially in urban areas. there is no evidence of coxiella burnetii infection in cats in japan prior to this. sera from 100 cats, collected in various parts of japan, were examined for antibody against c. burnetii. sixteen out of the 100 samples contained antibodies against c. burnetii. the prevalence of the antibody decreased from the northeastern to the southwestern part of japan. a high prevalence ... | 1994 | 7723682 |
| coxiella burnetii phase i and ii proteins studied by sds-page. | coxiella burnetii cells in both phase i and ii reveal in sodium dodecylsulphate-polyacrylamide gel electrophoresis (sds-page) similar protein profiles with only small differences. c. burnetii protein profile in sds-page depended on the method of purification of c. burnetii cells from chick embryo yolk sacs. immune mouse sera against c. burnetii phase i cells recognized in phase i and ii cell protein profiles mainly the 61 k and 29 k proteins by the immunoblot method. hyperimmune mouse and rabbit ... | 1994 | 7726000 |
| conventional viral cultures and shell vial assay for diagnosis of apparently culture-negative coxiella burnetii endocarditis. | a patient with culture-negative endocarditis was diagnosed with q fever endocarditis based on the results of serological tests and positive leukocyte cultures obtained using conventional viral cultures and the shell vial technique. this case report suggests that isolation of coxiella burnetii from blood may allow better diagnostic and therapeutical evaluation of patients with q fever endocarditis. the use of both conventional and shell vial viral cultures is recommended for the isolation of coxi ... | 1995 | 7729459 |
| prevalence of coxiella burnetii infection among slaughterhouse workers in northern spain. | | 1995 | 7729462 |
| cold-sensitive conditional mutations in era, an essential escherichia coli gtpase, isolated by localized random polymerase chain reaction mutagenesis. | conditional cold-sensitive mutations in era, an essential escherichia coli gtpase, were isolated. localized random polymerase chain reaction (pcr) mutagenesis employing taq and t7 dna polymerases under error prone amplification conditions was exploited to generate mutations in the era gene. a plasmid exchange technique was used to identify conditional cold-sensitive mutations in era that give rise to defective cell growth below 30 degrees c. three recessive missense mutations in era, n26s, a156d ... | 1995 | 7729673 |
| detection of coxiella burnetii in cow's milk using the polymerase chain reaction (pcr). | a pcr approach (transposon pcr) with primers based on repetitive transposon-like sequences, which--depending on the isolate--were found at a minimum frequency of 19 on the c. burnetii genome, was established for the highly sensitive and specific detection of c. burnetii. this study describes the analytical detection of c. burnetii in milk, which requires a special preparation method prior to pcr. because of the low level of c. burnetii particles in milk samples, template dna was concentrated by ... | 1994 | 7740857 |
| pathomorphological studies in european bison (bison bonasus linnaeus, 1758) with seropositive reaction to coxiella burnetii. | comprehensive serological and histopathological examinations of 47 free living european bison (bison bonasus linnaeus, 1758) were performed. of these animals, 36 were serologically positive due to coxiella burnetii, which confirmed the presence of q fever epizootic foci in this population of wild animals in poland. the presence of multiple foci of mononuclear cells typical for q fever was a consistent finding in all tissues of the majority of c. burnetii seropositive animals under study. pathomo ... | 1994 | 7740862 |
| characterization of the 23s and 5s rrna genes of coxiella burnetii and identification of an intervening sequence within the 23s rrna gene. | characterization of the rrna operon from the obligate intracellular bacterium coxiella burnetii has determined the order of the rrna genes to be 16s-23s-5s. a 444-bp intervening sequence (ivs) was identified to interrupt the 23s rrna gene beginning at position 1176. the ivs is predicted to form a stem-loop structure formed by flanking inverted repeats, and the absence of intact 23s rrna molecules suggests that the loop is removed. an open reading frame in the ivs has been identified that shows 7 ... | 1995 | 7751314 |
| is bell's palsy a reactivation of varicella zoster virus? | despite various pointers to an infectious aetiology, the cause of bell's palsy remains obscure. we examined paired sera from 62 patients with facial palsy and 50 age and sex matched contemporaneous controls. significantly more patients than controls had igm antibodies by elisa to varicella zoster virus (56.5% vs. 20%, p = 0.0001) and herpes simplex virus (41.9% vs. 18%, p = 0.006). additionally, significantly more patients than controls were positive for cf antibody to varicella zoster virus (14 ... | 1995 | 7751662 |
| [endocarditis in q fever: its serological diagnosis and variation in the coxiella burnetii phase]. | | 1995 | 7752721 |
| [pathomorphologic features in several phlebopathies associated with virus, rickettsia, chlamydia, and mycoplasma infections]. | seventy-six venous specimens were examined by indirect immunofluorescence and histological techniques. 80.26% out of them gave negative i.f. reactions. the following causal agents were found on the i.f. positive preparations: rickettsia burneti, chlamydia, mycoplasma, parainfluenza type 3 and adenoviruses, alone or associated. the most frequent lesion noted in i.f. positive specimens was of the inflammatory-dystrophic type. in i.f. negative preparations the dominant lesion was the hyalinosis of ... | 1994 | 7756163 |
| infective endocarditis in patients with negative blood cultures: analysis of 88 cases from a one-year nationwide survey in france. | blood cultures were negative in 88 (14%) of 620 cases of infective endocarditis (ie) documented in france during a 1-year nationwide survey. in 15 of these 88 cases, the causative microorganism was identified: seven cases of q fever endocarditis and two cases of chlamydial endocarditis were diagnosed by serological and/or immunohistologic techniques, and a pathogen was cultured from five surgically removed valves and one arterial septic embolus. forty-two (48%) of the 88 cases involved patients ... | 1995 | 7756467 |
| [occurrence of coxiella burnetti antibodies in cattle, sheep and small terrestrial mammals in the western region of bohemia]. | the prevalence of antibodies to c. burnetii was studied in the karlovy vary district (the czech republic) between 1987 and 1989. sera of cows, sheep and heart eluates of small ground mammals were tested using the complement fixation test with c. burnetii antigen in phase ii (bodibion, mevak nitra, slovakia). titers greater or equal to 8 or 10 were considered as positive. cows from two large-scale farms were tested four times (the average number of every sampling was 339 cows). their seroprevalen ... | 1995 | 7762122 |
| [q fever in the western region of ukraine]. | multiple natural and mixed foci of q fever have been revealed at the territory of six administrative regions of the western part of the ukraine. coxiella burnetii strains have been isolated from small animals and ticks, the specific features of their ecology in poles'e and forest-steppe zones have been determined. the necessity of the study of natural foci of q fever at not yet surveyed territories for at least 3-4 years has been substantiated. | 1995 | 7778371 |
| coxiella burnetii antibody prevalences among human populations in north-east africa determined by enzyme immunoassay. | retrospective serosurveys were conducted to determine the prevalence of antibody to phase-i coxiella burnetii among humans in various locations of north-east africa. sera were tested by the enzyme immunoassay (eia). initially the eia was compared with the standard indirect fluorescent antibody (ifa) method for the detection of antibody to c. burnetii. results indicated that the eia was slightly less sensitive (88%), but highly specific (94%) and less subjective than the ifa technique. eia was su ... | 1995 | 7783275 |
| necrotizing bronchitis, angiitis, and amyloidosis associated with chronic q fever. | the authors report the clinical, radiological and histological findings in a 63-year-old male patient who developed severe necrotizing bronchitis, necrotizing angiitis, and secondary amyloidosis of the right upper love and intermediate bronchus. the patient expired due to respiratory insufficiency. at the age of 27 years, the patient had had radiotherapy of the mediastinum because of suspected hodgkin's disease. acute pneumonia suggestive of q-fever infection was diagnosed at the age of 48. prog ... | 1995 | 7784709 |
| [antibodies to certain infections on large goat farms in the czech republic]. | on five newly established large goat farms the incidence of antibodies to some chronic and latent infections (arthritis and encephalitis, q-fever, caseous lymphadenitis and toxoplazmosis) was investigated in the first six months of the year 1994. agar-gel immunodiffusion did not reveal any antibodies to arthritis and encephalitis of goats (cae). complement fixation test did not demonstrate any antibodies to q-fever. neither agar-gel immunodiffusion nor neutralization test confirmed any antibodie ... | 1995 | 7793013 |
| protective immunity induced by 67 k outer membrane protein of phase i coxiella burnetii in mice and guinea pigs. | a 67 k outer membrane protein (omp) isolated from phase i coxiella burnetii qiyi strain was purified with monoclonal antibodies (moab) coupled to cnbr-sepharose 4b. chemical analyses of the 67 k protein showed that it contained seventeen kinds of amino acids and no lps. the immunogenicity and protectivity of the 67 k protein against c. burnetii was evaluated in mice and guinea pigs by in vitro lymphocyte proliferation assay, delayed-type skin test, antibody conversion rate, and immunization and ... | 1994 | 7793356 |
| immunogenicity of coxiella burnetii whole cells and their outer membrane components. | the immunogenicity and protective efficacy of the phase i and phase ii coxiella burnetii whole cells (cb i and cb ii) and their outer membrane components (omc), i.e. phase i trichloroacetic acid extract (tcae), phase i 29 k protein (pro), phase i and ii lipopolysaccharides (lps i, lps ii), polysaccharides (ps i, ps ii), and lipid a (la i, la ii), were compared. the highest immune response was observed in balb/c mice by cb i in both humoral immunity and lymphocyte transformation assays, and in th ... | 1994 | 7793358 |
| monoclonal antibody based competitive elisa for the detection of specific antibodies against coxiella burnetii in sera from different animal species. | a competitive elisa system for the detection of antibodies against coxiella (c.) burnetii in cattle, sheep, goats, horses and humans is described. the elisa is based on a biotinylated monoclonal antibody with specificity for c. burnetii lipopolysaccharide in combination with streptavidin peroxidase. for evaluation and statistical analysis, 413 sera from cattle, sheep, goats, horses and humans were tested in parallel in the indirect immunofluorescence test (ift). furthermore, a total of 448 bovin ... | 1994 | 7803931 |
| [q fever]. | | 1994 | 7808067 |
| prevalence of antibodies to coxiella burnetii in blood donors in the czech republic. | in 1988-1993, a total of 3,732 sera of blood donors from two districts (strakonice and nový jicń) of the czech republic were tested for antibodies to coxiella burnetii. using the complement-fixation test at titers of 8 (10) and higher, the antibodies were detected in 41 (1.1%). no statistically significant difference in seroprevalence between the strakonice and nový jicín districts was found (chi 2 = 2.536, p = 0.112, d.f. = 1). antibodies were ascertained in 36 donors, 24 of which worked in agr ... | 1994 | 7812248 |
| guillain-barré syndrome associated with acute q fever. | several bacterial and viral agents have been implicated in the pathogenesis of the guillain-barré syndrome, an acquired immune-mediated disorder. a case of guillain-barré syndrome associated with acute q fever is described. coxiella burnetii should therefore be added to the list of microorganisms capable of inducing the guillain-barré syndrome. | 1994 | 7813497 |
| characterization and protective effect of a 29 kda protein isolated from coxiella burnetii by detergent empigen bb. | a 29 kda protein, isolated from the outer membrane of coxiella burnetii, strain nine mile phase i by detergent empigen bb, was characterized. the failure in removing lipopolysaccharides (lps) from preparations of the protein by the purification method used indicates a strong binding between proteins and lps in the outer membrane of c. burnetii. the protein was immunogenic in mice and protected them against virulent c. burnetii challenge. | 1994 | 7813704 |
| a coxiella burnetii gene encodes a sensor-like protein. | two-component regulatory systems play important roles in the adaptive responses of many bacteria to environmental changes. the sensor proteins of these systems are highly conserved near their c-termini. we exploited this feature to isolate a gene encoding a putative sensor component from the obligate intracellular rickettsial parasite coxiella burnetii (cb). using degenerate primers and the polymerase chain reaction (pcr), we isolated a dna fragment from a genomic library of cb containing an ope ... | 1994 | 7828872 |
| cloning, sequencing and expression of the dnaj gene of coxiella burnetii. | a 6-kb ecori genomic dna fragment of coxiella burnetii, isolated from a recombinant bacteriophage lambda zapii library, allowed heterologous genetic complementation of escherichia coli deleted for its dnaj gene. the c. burnetii dnaj gene was expressed in e. coli and identified by western blot analysis using polyclonal antibodies raised against purified e. coli dnaj protein. deletion mapping and genetic complementation demonstrated that c. burnetii dnaj is present on a 2-kb ecori-hindiii genomic ... | 1995 | 7828937 |
| in vivo endogenous spore formation by coxiella burnetii in q fever endocarditis. | to determine whether coxiella burnetii, the aetiological agent of q fever, undergoes endogenous spore-like formation, the crucial stage of the developmental cycle, in the infected cardiac valves of patients with chronic q fever endocarditis. | 1994 | 7829692 |
| analysis of the rnc locus of coxiella burnetii. | a 3.2 kb ecori genomic dna fragment of coxiella burnetii was isolated by virtue of its ability to suppress mucoidy in escherichia coli. nucleotide sequence analysis revealed the presence of the genes homologous to rnc, era and reco of e. coli. suppression of capsule synthesis, measured by beta-galactosidase expression in lon- cps-lac fusion strains of e. coli, is caused by gene-dosage effects of the plasmid-borne rnc genes of either c. burnetii or e. coli. the rnc gene of c. burnetii complemente ... | 1994 | 7830573 |
| 'atypical' bacteria are a common cause of community-acquired pneumonia in hospitalised adults. | to assess the proportion of cases of community-acquired pneumonia caused by 'atypical' bacteria, including the recently discovered chlamydia pneumoniae, and to compare the clinical, radiographic and laboratory features of patients with and without 'atypical' bacteria. | 1994 | 7839256 |
| viral, mycoplasmal and chlamydial lower respiratory tract infections in hong kong: cost and diagnostic value of serology. | a hospital virology laboratory in hong kong tested 10,852 sera by complement fixation (cf) for antibodies against agents of respiratory disease between 1 july 1986 and 31 december 1991. ten commercially supplied antigens were used: influenza virus types a and b, parainfluenza virus types 1, 2 and 3, adenovirus, respiratory syncytial virus (rsv), mycoplasma pneumoniae, chlamydia species and coxiella burnetii. single sera comprised 69% of the total, including sera from 7488 patients investigated f ... | 1994 | 7841840 |
| analysis of the entire nucleotide sequence of the cryptic plasmid qph1 from coxiella burnetti. | the complete plasmid qph1 from coxiella burnetti, isolate 'nine mile', phase i, was cloned as noti fragment with a size of 37329 bp. the entire plasmid was sequenced by the chain termination method after ecori subcloning. 37 open reading frames coding for polypeptides larger than 100 amino acid residues were determined. the predicted polypeptide products of the open reading frames were compared by computer analysis with reported protein sequences. homologies of predicted polypeptide products to ... | 1994 | 7843345 |
| is plasmid based differentiation of coxiella burnetii in 'acute' and 'chronic' isolates still valid? | ten human coxiella burnetii isolates from french patients with acute hepatitis or chronic endocarditis were characterized according to their polymorphism in dna restriction patterns and differentiated by plasmid-specific pcr. the aim of this investigation was to clarify if the present classification of so called 'acute' and 'chronic' coxiella burnetii isolates--based on plasmid profile of a so far limited number of partly ancient isolates--could be confirmed with lately isolated organisms of thi ... | 1994 | 7843346 |
| q fever (coxiella burnetii) reservoir in wild brown rat (rattus norvegicus) populations in the uk. | seroprevalence of coxiella burnetii, the causal agent of q fever in humans, was examined in wild brown rat populations from 4 oxfordshire farmsteads (n = 127), 9 somerset homesteads (n = 98), and 1 captive enclosure (n = 88) in the uk. seroprevalence ranged between 7 and 53%, the lowest being within the captive population and the highest on dairy farmsteads with sheep present. this is the first reported investigation of c. burnetii in wild rats outside india. we suggest that the high seroprevale ... | 1995 | 7845709 |
| [pericarditis and q fever]. | | 1994 | 7865665 |
| [liver involvement in q fever and mediterranean boutonneuse fever. comparative study]. | rickettsia spp. infections produce hepatic damage with transaminases elevation and biological signs of cholostasis. classical biochemical tests of hepatic function were analyzed and compared in 8 patients with q fever (qf) and 7 with boutonneuse mediterranean fever (bmf). liver enlargement was detected in 75% of the qf group of patients as compared with the 57% of the bmf group. transaminases were raised in 75% of the patients of the qf group and in 85, 7% of the bmf patients. only one patient i ... | 1994 | 7873263 |
| a new plasmid (qpdv) common to coxiella burnetii isolates associated with acute and chronic q fever. | genetic studies of coxiella burnetii strains suggested the possibility of differentiating new isolates according to their plasmid dna content. virulence and/or clinical manifestations ('chronic' and 'acute' q fever) had been claimed to correlate with this plasmid typing. a new plasmid, named qpdv, was found to be common to c. burnetii isolates obtained from acute and chronic q fever. according to the results obtained, plasmid usage for detection and differentiation of respective pathovars of c. ... | 1995 | 7875575 |
| [the morphological changes in the body of guinea pigs in response to the administration of different coxiella burnetii antigens]. | in this work the evaluation of macro- and micro-changes in the tissues of guinea pigs to the injection of inactivated antigens of c. burnetii is presented. the injection of these antigens in a dose of 50 micrograms induced only an insignificant reaction at the site of injection; in the brain of the animals, as well as in all examined internal organs, no visible pathological changes were present. during the first two weeks after the injection of the antigens signs of irritation of the reticuloend ... | 1994 | 7879483 |
| [the importance of serological methods in the diagnosis of q fever in western ukraine]. | | 1994 | 7879492 |
| comparison of various methods of lipopolysaccharide isolation from coxiella burnetii strain priscilla in the virulent phase i. | four methods of isolation of a lipopolysaccharide (lps) from coxiella burnetii strain priscilla in virulent phase i have been examined. each isolation method afforded a specific lps portion of the polydisperse lps system present in the outer membrane of c. burnetii cell. the hot phenol/water method was found the most efficient with respect to yield and chemical composition of the smooth (s) lps that was shown to prevail in the outer membrane lps macromolecules of c. burnetii. | 1994 | 7879711 |
| analysis of the 3-deoxy-d-manno-2-octulosonic acid region in a lipopolysaccharide isolated from coxiella burnetii strain nine mile in phase ii. | structural analysis of the 3-deoxy-d-manno-2-octulosonic acid (kdo) region in a lipopolysaccharide (lps) ii isolated from coxiella burnetii strain nine mile in avirulent phase ii revealed the presence of three variously linked kdo residues. the lipid a proximal kdo is substituted at c-4 by a kdo-(2-->4)-kdo disaccharide and this structural arrangement of the kdo residues is similar to that of enterobacterial lpss. | 1994 | 7879717 |
| survival of the q fever agent coxiella burnetii in the phagolysosome. | the q fever agent, coxiella burnetii, thrives in the acidic environment of the phagolysosome of the host cell. how this obligate intracellular agent manages to survive within this hostile milieu is unknown; however, several of its enzymes may eliminate or prevent the formation of toxic oxygen metabolites by the host cell. also implicated as virulence factors are its surface lipopolysaccharide and plasmids. | 1994 | 7889323 |
| a refined guinea pig model for evaluating delayed-type hypersensitivity reactions caused by q fever vaccines. | the previously established model for determining dermal granulomatous reactivity of q fever vaccines uses footpad injections to sensitize hartley guinea pigs. we describe a substantial refinement to that model by substituting an alternate injection route and replacing the model with the hairless hartley guinea pig. the experimental design incorporates a matrix of various antigen and adjuvant combinations administered by subcutaneous (sc) and intradermal routes of administration. the two coxiella ... | 1994 | 7898035 |
| [anti-cardiolipin antibodies in acute coxiella burnetii infection. value of the search and course of different isotypes]. | | 1995 | 7899425 |
| polymorphism in dna restriction patterns of coxiella burnetii isolates investigated by pulsed field gel electrophoresis and image analysis. | pulsed field gel electrophoresis was used for differentiation of coxiella burnetii isolates derived from animals in europe and one from namibia. previously published data on isolates from north america demonstrated four groups with distinct banding patterns. fingerprints of prototype isolates as references confirmed four different cleavage patterns upon noti restriction of total dna as described. with isolates available at our institute five additional dna banding patterns were obtained from eur ... | 1993 | 7902294 |
| rickettsiae and their ecology in the alpine region. | two rickettsioses are known to be distributed in the alpine region, the ubiquitous q fever and the so called tick-bite fever (fever boutonneuse) which is due to rickettsia conorii. the latter is only temporarily seen and brought in via dogs by the dog tick rhipicephalus sanguineus from italy and southern france. further, the presence of two apathogenic rickettsiae, r. slovaca (in austria, switzerland, germany) and r. helvetica (switzerland) has been determined. the present paper will discuss eco ... | 1993 | 7905244 |
| [characteristics of dna restriction fragment length polymorphisms of c. burnetti strains, isolated from areas of russia]. | the structural heterogeneity in coxiella burnetii chromosomal dna isolated in the european part of russia from people, agricultural animals, and ticks has been studied. it is compared with the one of the european strains henzerling and m44, the only genetically characterized strains up to date. the digestion of the total dna by the restriction endonucleases bamhi, psti, xhoi resulted in obtaining two types of restriction patterns. the ones for henzerling and m44 differed from the restriction pat ... | 1993 | 7905598 |
| acute q fever: first case report in taiwan. | a 70-year-old retired fisherman presented with fever, chills and headache for four days. the clinical presentation was mild, including a self-limiting fever, pneumonia and elevated liver enzyme levels. acute q fever was proved by a four-fold rise of phase ii igg antibodies. interestingly, the man denied any animal contact or travelling over the past year. this case may suggest that q fever should be added to the list of differential diagnoses for acute febrile disease in taiwan. | 1993 | 7908577 |