| production of human papillomavirus type 16 virus-like particles in transgenic plants. | cervical cancer is linked to infection with human papillomaviruses (hpv) and is the third most common cancer among women worldwide. there is a strong demand for the development of an hpv preventive vaccine. transgenic plants expressing the hpv major capsid protein l1 could be a system to produce virus-like particles for prophylactic vaccination or could even be used as edible vaccines to induce an l1-specific prophylactic immune response. here, we describe the generation of transgenic tobacco an ... | 2003 | 12915537 |
| biomimetic organization: octapeptide self-assembly into nanotubes of viral capsid-like dimension. | the controlled self-assembly of complex molecules into well defined hierarchical structures is a promising route for fabricating nanostructures. these nanoscale structures can be realized by naturally occurring proteins such as tobacco mosaic virus, capsid proteins, tubulin, actin, etc. here, we report a simple alternative method based on self-assembling nanotubes formed by a synthetic therapeutic octapeptide, lanreotide in water. we used a multidisciplinary approach involving optical and electr ... | 2003 | 12930900 |
| activation of hypersensitive response genes in the absence of pathogens in transgenic tobacco plants expressing a rice small gtpase. | transgenic tobacco ( nicotiana tabacum l.) plants constitutively expressing a rice ( oryza sativa l.) gene encoding a small gtpase, rgp1, showed marked resistance to tobacco mosaic virus (tmv) infection compared with the wild type [h. sano et al. (1994) proc natl acad sci usa 91:10556-10560]. in order to examine the gene expression profile, the temperature-shift method was adopted to hyper-activate the n-gene inducing the hypersensitive response (hr), and transcripts of 11 representative hr gene ... | 2003 | 12937984 |
| estimation of population bottlenecks during systemic movement of tobacco mosaic virus in tobacco plants. | more often than not, analyses of virus evolution have considered that virus populations are so large that evolution can be explained by purely deterministic models. however, virus populations could have much smaller effective numbers than the huge reported census numbers, and random genetic drift could be important in virus evolution. a reason for this would be population bottlenecks during the virus life cycle. here we report a quantitative estimate of population bottlenecks during the systemic ... | 2003 | 12941900 |
| [a point mutation in the coat protein gene affects long distance transport of the tobacco mosaic virus]. | a mutation resulting in substitution of positively charged lys53 with negatively charged glu in the coat protein was introduced in the infectious cdna copy of the genome of wild-type tobacco mosaic virus strain u1. kinetic analysis of long-distance virus transport in plants showed that systemic distribution of the mutant virus was delayed by 5-6 days as compared with the wild-type one. on evidence of rna sequencing in the mutant progeny, glu50 of the coat protein was substituted with lys after p ... | 2003 | 12942648 |
| [changes in ultrastructure and protein metabolism of tobacco plants infected by tomato spotted wilt virus]. | as the result of electron microscope investigation of ultra-thin sections of the tissues infected by tomato spotted wilt virus it was shown that ultrastructural changes in the cells depend on the virus virulence. the isolate with low virulence induces mostly virus-specific changes (virus particles and virus inclusion bodies); the isolate with high virulence besides the virus-specific changes causes essential non-specific violation of cell organelle structure that could be the consequence of path ... | 2003 | 12945179 |
| phenylpropanoid compounds and disease resistance in transgenic tobacco with altered expression of l-phenylalanine ammonia-lyase. | tobacco plants over-expressing l-phenylalanine ammonia-lyase (pal(+)) produce high levels of chlorogenic acid (cga) and exhibit markedly reduced susceptibility to infection with the fungal pathogen cercospora nicotianae, although their resistance to tobacco mosaic virus (tmv) is unchanged. levels of the signal molecule salicylic acid (sa) were similar in uninfected pal(+) and control plants and also following tmv infection. in crosses of pal(+) tobacco with tobacco harboring the bacterial nahg s ... | 2003 | 12946414 |
| individualized human scfv vaccines produced in plants: humoral anti-idiotype responses in vaccinated mice confirm relevance to the tumor ig. | we have developed a method for rapidly producing in plants the idiotype regions of the tumor-specific ig as single-chain fv (scfv) proteins for use in the treatment of non-hodgkin's lymphoma. variable region gene sequences were generated from either a tumor hybridoma or human tumor biopsy cells, and idiotype domains were joined by a novel linker and cloned into a modified tobacco mosaic virus (tmv) vector designed to secrete the scfv protein in infected nicotiana benthamiana plants. thirty-eight ... | 2003 | 12957399 |
| the nucleic acids of some strains of tobacco mosaic virus. | | 1952 | 12981054 |
| infra-red dichroism of tobacco mosaic virus nucleoprotein. | | 1952 | 12993206 |
| [effect of inhibitors of nucleic metabolism on the multiplication of tobacco mosaic virus]. | | 1952 | 12998548 |
| action of carboxypeptidase on tobacco mosaic virus. | | 1952 | 13002379 |
| immunochemical studies on tobacco mosaic virus. vii. the quantitative precipitin reaction with equine antiserum. | | 1952 | 13011310 |
| immunochemical studies on tobacco mosaic virus. viii. the specificity of chemically-altered virus. | | 1952 | 13011311 |
| solution of tobacco mosaic virus in the aqueous phase of a chloroform-water emulsion and application of this phenomenon in virus assay. | | 1953 | 13028270 |
| [effect of formaldehyde derivative of norsulfazol on tobacco mosaic virus]. | | 1953 | 13033719 |
| cytochemical studies on chloroplasts. ii. inhibitory effects of chloroplast-suspension upon the infectivity of tobacco mosaic virus. | | 1952 | 13033866 |
| occurrence in plants infected with tobacco mosaic virus of a crystallizable antigen devoid of ribonucleic acid. | | 1953 | 13054781 |
| inhibition of tobacco mosaic virus biosynthesis by 2-thiopyrimidines. | | 1953 | 13056613 |
| differentiation of tobacco mosaic virus strains by differences in ph of maximum optical density. | | 1953 | 13058419 |
| the identity of the purine-bound pentose of some strains of tobacco mosaic virus. | | 1953 | 13061429 |
| a comparison of some mutants of tobacco mosaic virus. | | 1953 | 13061430 |
| incorporation of 8-azaguanine into nucleic acid of tobacco mosaic virus. | | 1953 | 13063527 |
| [size of particles of tobacco mosaic virus during various periods of reproduction and during nitrogen lact in plant-host]. | | 1953 | 13068269 |
| the effects of tobacco mosaic virus synthesis on the free amino acid and amide composition of the host. | 1. changes in concentrations of free amino acids and amides have been determined in tmv-infected tobacco leaf discs and in comparable uninfected discs during the time of virus formation. 2. during the period of rapid virus formation the infected discs show a transitory deficiency (as compared to uninfected discs) in glutamine, asparagine, aspartic acid, glutamic acid, serine, and to a lesser extent in valine, threonine, and proline. about 100 hours before this time smaller deficiencies in the co ... | 1953 | 13069682 |
| relationships between tobacco mosaic virus biosynthesis and the nitrogen metabolism of the host. | 1. comparisons of the nitrogen content of tmv-infected and uninfected tobacco leaf discs at various times after inoculation show that virus synthesis is associated with a net increase in protein content. this excess protein is due to: (a) tmv, (b) an excess in insoluble protein which develops soon after inoculation and ends about 100 hours before cessation of tmv synthesis, and (c) an excess in soluble non-virus protein, which is variable in size and which only occurs during the time of virus sy ... | 1953 | 13069683 |
| [study of normal ribonucleoproteins of leaves infected with tobacco mosaic virus]. | | 1953 | 13081231 |
| [reaction of desoxyribonucleic acids and tobacco mosaic virus with methyl green and pyronine]. | | 1953 | 13081238 |
| [spiral structure of fragments of tobacco mosaic virus]. | | 1953 | 13083501 |
| [aggregation of tobacco mosaic virus in plant cells in early stages of reproduction]. | | 1953 | 13083516 |
| radioautographic study of the localization of tobacco mosaic virus antigen. | | 1953 | 13087258 |
| [new methods of detecting a soluble antigen of tobacco mosaic virus]. | | 1953 | 13093084 |
| the breaking of tobacco mosaic virus using a new freeze drying method. | | 1953 | 13093740 |
| incorporation of 2-thiouracil-s35 in the ribose nucleic acid of tobacco mosaic virus. | | 1953 | 13093750 |
| the proteins synthesized in tissue infected with tobacco mosaic virus. | | 1953 | 13113168 |
| biophysical studies of the ultrasonic fragmentation of tobacco mosaic virus. | | 1953 | 13114912 |
| [effect of nitrogenous substances on the multiplication of tobacco mosaic virus in tissue culture]. | | 1953 | 13116580 |
| [correlation of tobacco mosaic virus with myosin and actin]. | | 1953 | 13116901 |
| a screening test of the effect of organic compounds on production of tobacco mosaic virus. | | 1954 | 13117821 |
| some effects of sucrose and phosphorus in increasing the multiplication of tobacco mosaic virus in detached tobacco leaves. | | 1953 | 13118094 |
| [inhibition of the tobacco mosaic virus by the capsular polyoside of torulopsis neoformans]. | | 1953 | 13124923 |
| the fission of tobacco mosaic virus and some other nucleoproteins by strontium nitrate. | | 1954 | 13126096 |
| effect of iodine on serological specificity of tobacco mosaic virus. | | 1954 | 13134321 |
| a differential ability of strains of tobacco mosaic virus to bind host-cell nucleoprotein. | | 1954 | 13135521 |
| stability of chymotrypsin and tobacco mosaic virus decreased by ultraviolet radiation. | | 1954 | 13140199 |
| the structure of tobacco mosaic virus. i. x-ray evidence of a helical arrangement of sub-units around the longitudinal axis. | | 1954 | 13140277 |
| influence of thiouracil incorporation in the ribonucleic acid moiety of tobacco mosaic virus on its multiplication. | | 1954 | 13140301 |
| [phenomenon of interference between tobacco mosaic virus and virus x of potatoes in streak diseases of tomatoes]. | | 1954 | 13141455 |
| [radioautographic study of the cellular localization of tobacco mosaic virus used as an antigen]. | | 1954 | 13158962 |
| the inheritance of antibody response to tobacco mosaic virus in rabbits. | | 1954 | 13159755 |
| [morphological and cytochemical investigations on tobacco mosaic virus-infected protoplasts of nicotiana tabacum]. | | 1954 | 13173518 |
| effects of some purine analogues on tobacco mosaic virus. | | 1954 | 13174776 |
| the effect of a synthetic lysine polypeptide upon the velocity of precipitation of tobacco mosaic virus by its antiserum. | | 1954 | 13174807 |
| [detection and properties of non-infectious tobacco mosaic virus and of virus deprived of ribonucleic acid]. | | 1954 | 13181888 |
| genetic composition in relation to isoelectric point and serological reaction of strains in tobacco mosaic virus. | | 1954 | 13187557 |
| [applicability of datura stramonium for the determination of titer of tobacco mosaic virus]. | | 1953 | 13193554 |
| auxiliary infectious nucleoprotein from plants infected with tobacco mosaic virus. | | 1954 | 13216209 |
| the purine and pyrimidine composition of the tobacco mosaic virus and the holmes masked strain. | | 1954 | 13221558 |
| the role of the soluble antigens in the multiplication of the tobacco mosaic virus. | | 1955 | 13239652 |
| [attempted direct infection of the parenchymal cells of tobacco leaves by means of tobacco mosaic virus labeled with radiocarbon and radiophosphorus; exit of the virus]. | | 1955 | 13239663 |
| deuteron bombardment of oriented tobacco mosaic virus preparations. | | 1955 | 13246640 |
| visible and ultraviolet light scattering by tobacco mosaic virus nucleic acid. | | 1955 | 13249995 |
| the inhibiting effect of some heterocyclic and other organic compounds on tobacco mosaic virus. | | 1955 | 13250448 |
| distribution of radiocarbon in tobacco mosaic virus. | | 1955 | 13253556 |
| terminal groups of tobacco mosaic virus. | | 1955 | 13253590 |
| [effect of vegetative correlation between infections of nicotiniana tabacum l. and n. glutinosa l. with tobacco mosaic virus]. | | 1955 | 13261194 |
| mechanism of the action of abrasives on infection by tobacco mosaic virus. | | 1955 | 13267982 |
| comparison of some physical and chemical properties of eight strains of tobacco mosaic virus. | | 1955 | 13267993 |
| are cucumber viruses 3 and 4 strains of tobacco mosaic virus? a review of the problem. | | 1955 | 13267994 |
| the reaction of tobacco mosaic virus with iodine. | | 1955 | 13271401 |
| anomalous proteins associated with three strains of tobacco mosaic virus. | | 1955 | 13274740 |
| zinc nutrition of nicotiana tabacum l. in relation to multiplication of tobacco mosaic virus. | | 1955 | 13274743 |
| c-terminal amino acid sequences of four strains of tobacco mosaic virus. | | 1955 | 13275975 |
| structural resemblance between schramm's repolymerised a-protein and tobacco mosaic virus. | | 1955 | 13276395 |
| studies on the aggregation reactions and basic dye binding of tobacco mosaic virus. i. variation of ph, particle asymmetry, acid and base titration results, irreversible binding of methylene blue, ultraviolet absorption, and extent of heat denaturation in tobacco mosaic virus solutions with time of standing. | 1. aqueous solutions of tobacco mosaic virus were found to undergo a number of spontaneous changes on standing in the cold. the results of ph measurements, acid and base titrations, intrinsic viscosity determinations, studies on the irreversible binding of methylene blue with the virus, ultraviolet absorption, and the extent of nucleic acid splitting by heat denaturation indicated the occurrence of two successive reactions, the first one causing the release of hydrogen ions and a greater labilit ... | 1956 | 13286459 |
| reactivation of partially degraded tobacco mosaic virus. | | 1956 | 13288611 |
| reversible, host-induced, changes in a strain of tobacco mosaic virus. | | 1956 | 13297028 |
| the effect of tobacco mosaic virus biosynthesis on the nucleic acid content of tobacco leaf. | | 1956 | 13299699 |
| cumulative concentrations of tobacco mosaic virus in tobacco and tomato at different temperatures. | | 1956 | 13299700 |
| cumulative concentrations of tobacco mosaic virus in tobacco at different photoperiods and light intensities. | | 1956 | 13299701 |
| the inactivation of the infectious centers of tobacco mosaic virus by ultraviolet light. | | 1956 | 13299703 |
| as estimate of the number of tobacco mosaic virus particles in a single hair cell. | | 1956 | 13299707 |
| reactivation of tobacco mosaic virus infectivity in mixtures of virus protein and nucleic acid. | | 1956 | 13304100 |
| x-ray diffraction studies of cucumber virus 4 and three strains of tobacco mosaic virus. | | 1956 | 13315264 |
| thiouracil in tobacco mosaic virus. | | 1956 | 13315324 |
| [structure of tobacco mosaic virus proteins]. | | 1956 | 13315335 |
| [studies on reproduction of tobacco mosaic virus. i. electron microscopic observations]. | | 1956 | 13317912 |
| observations on the anomalous proteins occurring in extracts from plants infected with strains of tobacco mosaic virus. | | 1956 | 13319650 |
| [multiplication of tobacco mosaic virus in bacterial tumors of tomatoes]. | | 1956 | 13321549 |
| infectivity of ribonucleic acid from tobacco mosaic virus. | | 1956 | 13321939 |
| mechanism of inactivation of tobacco mosaic virus by x-ray. | | 1956 | 13321988 |
| does the aphid myzus persicae (sulz.) imbibe tobacco mosaic virus? | | 1956 | 13325405 |
| some components of tobacco mosaic virus preparations made in different ways. | | 1956 | 13328828 |
| morphological changes accompanying thermal denaturation of tobacco mosaic virus. | | 1956 | 13328865 |
| further studies concerning the breaking of tobacco mosaic virus. | | 1956 | 13341940 |
| the separation of strains of tobacco mosaic virus by continuous filter-paper electrophoresis. | | 1956 | 13341951 |
| [problem of ontogenesis of tobacco mosaic virus]. | | 1956 | 13352964 |
| x-ray studies on tobacco mosaic virus. | | 1956 | 13355473 |
| structural studies on the nucleic acids of some strains of tobacco mosaic virus. i. the residual material after ribonuclease digestion. | | 1956 | 13357457 |
| a study of chromosomes with the electron microscope. | amphibian lampbrush chromosomes and meiotic prophase chromosomes of various insects and plants consist of a bundle of microfibrils about 500 a thick. these fibrils are double, being made of two closely associated fibrils 200 a thick. fragments of interphase nuclei contain a mass of fibrils 200 a thick. ultrathin sections through nuclei in prophase or interphase show sections of these double or single fibrils cut at various angles. a comparison of sections with the methacrylate left in and sectio ... | 1956 | 13357574 |