| [polymorphism of microsatellite markers in breeds of common carp (cyprinus carpio l.) of russian breeding]. | using five microsatellite loci, genotyping and genetic diversity estimates were obtained for nine samples representing seven common carp breeds most widespread in russia. for comparison, the samples of amur wild common carp (cyprinus carpio haematopterus) and a sample of european hungarian common carp were used. in the samples examined (n = 148) a total of 78 alleles were revealed. the highest mean allele number per locus (4.3) was identified in amur wild common carp, while the lowest number was ... | 2010 | 20583601 |
| a novel mechanism of cell growth regulation by cell cycle and apoptosis regulatory protein (carp)-1. | abstract: | 2010 | 20594350 |
| [investigations on ligula intestinalis plerocercoid l., 1758 infection of some fishes from dam lake kunduzlar (kirka, eskişehir).]. | in this study, the presence of ligula intestinalis plerocercoid in some fish species (cyprinus carpio, carassius gibelio, barbus plebejus, capoeta tinca, chondrostoma nasus, leuciscus cephalus, alburnus escherichii) from the kunduzlar dam, turkey was investigated between august 2008 and may 2009. by the end of the study, ligula intestinalis plerocercoid haqs been identified in the body cavity of 3 host fishes [chondrostoma nasus (1.1%, 5.0+/-0), leuciscus cephalus (12.1%, 1.5+/-0,6), and alburnu ... | 2010 | 20597057 |
| a novel role for cardiac ankyrin repeat protein ankrd1/carp as a co-activator of the p53 tumor suppressor protein. | the muscle ankyrin repeat protein (marp) family member ankrd1/carp is a part of the titin-mechanosensory signaling complex in the sarcomere and in response to stretch it translocates to the nucleus where it participates in the regulation of cardiac genes as a transcriptional co-repressor. several studies have focused on its structural role in muscle, but its regulatory role is still poorly understood. to gain more insight into the regulatory function of ankrd1/carp we searched for transcription ... | 2010 | 20599664 |
| common carp have two subclasses of bonyfish specific antibody igz showing differential expression in response to infection. | immunoglobulin heavy chains identified in bony fish are broadly classified into three classes namely igm, igd and igz. the most recently described isotype is igz, a teleosts-fish specific isotype that shows variations in gene structure across teleosts. in this study we have identified two igz subclasses in common carp. igz1 is a four constant heavy chain domains containing antibody isolated across teleosts and igz2 is a two constant domains containing heavy chain chimera with a μ1 and ζ4 domain. ... | 2010 | 20600275 |
| characterization of c1q in teleosts: insight into the molecular and functional evolution of c1q family and classical pathway. | c1qs are key components of the classical complement pathway. they have been well documented in human and mammals, but little is known about their molecular and functional characteristics in fish. in the present study, full-length cdnas of c1qa, c1qb, and c1qc from zebrafish (danio rerio) were cloned, revealing the conservation of their chromosomal synteny and organization between zebrafish and other species. for functional analysis, the globular heads of c1qa (gha), c1qb (ghb), and c1qc (ghc) we ... | 2010 | 20615881 |
| over-expression of the dclk gene transcript carp decreases ca3/ca1 network excitability. | products of the doublecortin like kinase (dclk) gene are implicated in cortical migration and hippocampal maturation during embryogenesis. however, one of its splice variants, called camk related peptide (carp), is expressed during adulthood in response to neurological stimuli, such as kainic acid-induced seizures and bdnf-ltp. the function of this transcript of the dclk gene is poorly understood. to elucidate its function during adulthood we have created transgenic mice with over-expression of ... | 2010 | 20659433 |
| a multivariate comparison of the stress response in three salmonid and three cyprinid species: evidence for inter-family differences. | the response of six species of freshwater fishes, from the families cyprinidae (common carp cyprinus carpio, roach rutilus rutilus and chub leuciscus cephalus) and salmonidae (rainbow trout oncorhynchus mykiss, brown trout salmo trutta and arctic charr salvelinus alpinus), to a standardized stressor was evaluated. a 6 h period of confinement resulted in changes to plasma cortisol, glucose, amino acid and lactate levels compared with unconfined controls. there were significant differences in the ... | 2010 | 20666900 |
| regulation of ovarian steroidogenesis in vitro by gonadotropin in common carp cyprinus carpio: interaction between calcium- and adenylate cyclase-dependent pathways and involvement of erk signaling cascade. | multiple signal transduction pathways mediating gonadotropin-induced testosterone and 17β-estradiol (e(2)) production were identified in carp ovarian theca and granulosa cells in short-term co-incubation. inhibitors of voltage-sensitive calcium channels (vsccs) and calmodulin attenuated human chorionic gonadotropin (hcg)-induced steroid production, whereas modulators of adenylate cyclase and protein kinase a (pka) increased their production, indicating that both calcium- and pka-dependent pathwa ... | 2010 | 20668068 |
| hypertrophy, gene expression, and beating of neonatal cardiac myocytes are affected by microdomain heterogeneity in 3d. | cardiac myocytes are known to be influenced by the rigidity and topography of their physical microenvironment. it was hypothesized that 3d heterogeneity introduced by purely physical microdomains regulates cardiac myocyte size and contraction. this was tested in vitro using polymeric microstructures (g' = 1.66 gpa) suspended with random orientation in 3d by a soft matrigel matrix (g' = 22.9 pa). after 10 days of culture, the presence of 100 μm-long microstructures in 3d gels induced fold increas ... | 2010 | 20668947 |
| [phylogenetic relationships among cobitoidea based on mitochondrial nd4 and nd5 gene sequences]. | in the present study, we cloned and sequenced 10 new nd4 and nd5 gene sequences of cobitoidea. these sequences were used to reconstruct phylogenetic relationships together with those of 15 other species downloaded from genbank. the results showed that the length of nd4 gene sequence was 1 380-1 387 bp with atg as starting codon and incomplete termination signal as terminated codon; the length of nd5 gene sequence was 1 821-1 839 bp with atg as starting codon and taa or tag as terminated codon; t ... | 2010 | 20672409 |
| interspecies correlations of toxicity to eight aquatic organisms: theoretical considerations. | interspecies correlations allow the prediction of toxicity to a number of other species. however, little attention has been paid to the theoretical considerations of the interspecies relationship based on the differences of bio-uptake and toxic mechanism between species. this study examines the interspecies correlations of toxicity between species of vibrio fischeri, river bacteria, algae, daphnia magna, carp, tetrahymena pyriformis, fathead minnow and guppy based on the theoretical background. ... | 2010 | 20673582 |
| detection of cyprinid herpesvirus-3 dna in lake plankton. | the disease caused by cyprinid herpesvirus-3 (cyhv-3) severely impacts the natural freshwater ecosystem and damages carp and koi farming, however, the pathway of cyhv-3 transmission remains unclear. it is possible that the virus adheres to plankton, which then facilitate viral movement and transmission, and therefore, it is hypothesised that plankton are involved in the disease dynamics. in this study, plankton were collected at eight sites in the iba-naiko lagoon; we detected and quantified cyh ... | 2011 | 20692005 |
| vitreoscilla hemoglobin (vhb) overexpression increases hypoxia tolerance in zebrafish (danio rerio). | aquaculture farming may benefit from genetically engineering fish to tolerate environmental stress. here, we used the vector pcvcg expressing the vitreoscilla hemoglobin (vhb) gene driven by the common carp β-actin promoter to create stable transgenic zebrafish. the survival rate of the 7-day-old f(2) transgenic fish was significantly greater than that of the sibling controls under 2.5% o(2) (dissolved oxygen (do), 0.91 mg/l). meanwhile, we investigated the relative expression levels of several ... | 2011 | 20694827 |
| a unique feature of toll/il-1 receptor domain-containing adaptor protein is partially responsible for lipopolysaccharide insensitivity in zebrafish with a highly conserved function of myd88. | myd88 and toll/il-1r domain-containing adaptor protein (tirap) are required for the tlr4 response to lps stimulation in mammals, but the functions of the two adaptors and their involvement in zebrafish insensitivity to lps remains unknown. we present a functional analysis of zebrafish myd88 and tirap and suggest that myd88 is more important than tirap for the activation of tlr-mediated nf-kappab, which may be a novel mechanism of myd88-dependent tlr signaling in teleosts. zebrafish tirap lacks t ... | 2010 | 20702732 |
| cloning, characterization, and expression analysis of toll-like receptor-7 cdna from common carp, cyprinus carpio l. | the toll-like receptor 7 (tlr7) is activated by single strand rna and rna-like compounds (imidazoquinoline), and it induces interferon production. we identified and described carp tlr7 cdna and its mrna expression. the full-length cdna of carp tlr7 gene is 3427 bp, encoding 1049 amino acids (ab553573). the similarities of carp tlr7 with zebrafish, rainbow trout, fugu, and human tlr7 were 89.6, 83.4, 80.6 and 74.6%, respectively, at the amino acid sequence level. furthermore, the expression of tl ... | 2010 | 20709611 |
| development and evaluation of a sensitive and quantitative assay for hirame rhabdovirus based on quantitative rt-pcr. | the aim of the present work was to develop a quantitative reverse-transcription polymerase chain reaction (qrt-pcr) assay using a taqman probe to detect and quantify hirame rhabdovirus (hrv). the results demonstrated that the assay had a detection limit of 100 copies of rna per reaction and a log-linear range up to 10(8) copies of hrv rna. regression analysis demonstrated a significant correlation with an r(2) value of 0.9963 and a slope of -3.18 between the mean c(t) values and hrv crna. this a ... | 2010 | 20723563 |
| transmission of cyprinid herpesvirus-3 (cyhv-3) from goldfish to naïve common carp by cohabitation. | cyprinid herpesvirus-3 (cyhv-3) has spread worldwide and has had a major impact on koi and common carp production. previous studies on the host range of the cyhv-3 found that fish species other than koi and common carp are fully resistant to natural virus exposure. recently, cyhv-3 was detected in goldfish (carassius auratus auratus) that were in contact with cyhv-3 infected koi. in the present study, a specific rt-pcr product was amplified from the viral thymidine kinase gene in gills, intestin ... | 2011 | 20723951 |
| effects of crude oil and oil fractions on the liver p450-dependent monooxygenase activities and antioxidant defence system of different freshwater fish species. | the effects of crude oil (szeged-algyo, hungary) and oil fractions (f1: rich in aromatics; f2 fraction: free from aromatics) were investigated on liver cyp1a isoenzymes and antioxidant defence system following their i.p. injection into different freshwater fish species: carp (cyprinus carpio l.), silver carp (hyphothalmichtys molitrix v.), and european eel (anquilla anquilla). a dose of 2 ml kg -1 crude oil enhanced erod activity 8-fold in carp and only 5-fold in eel after 3 days. oil fraction f ... | 2010 | 20724273 |
| development and characterization of three new diploid cell lines from labeo rohita (ham.). | development of cell lines from fish for identifying the pathogenesis of viral diseases and for vaccine production against viral and bacterial diseases is imperative where they are of commercial importance. three new diploid fish cell lines (rf, rh, and rsb) were developed from fin, heart, and swim bladder of an indian major carp, labeo rohita, commonly called rohu. all the cell lines were optimally maintained at 28 degrees c in leibovitz-15 medium supplemented with 10% fbs. the propagation of rh ... | 2010 | 20730759 |
| transmission dynamics of an emerging infectious disease in wildlife through host reproductive cycles. | emerging infectious diseases are major threats to wildlife populations. to enhance our understanding of the dynamics of these diseases, we investigated how host reproductive behavior and seasonal temperature variation drive transmission of infections among wild hosts, using the model system of cyprinid herpesvirus 3 (cyhv-3) disease in common carp. our main findings were as follows: (1) a seroprevalence survey showed that cyhv-3 infection occurred mostly in adult hosts, (2) a quantitative assay ... | 2011 | 20740025 |
| inclusion of atlantic salmon in the chinese diet reduces cardiovascular disease risk markers in dyslipidemic adult men. | although the beneficial effects of n-3 fatty acids on several physiologic functions have been widely reported, information about the effects of oily fish in the asian diet on cardiovascular disease (cvd) risk is diminutive. we hypothesize that daily inclusion of oily fish for 8 weeks in the chinese diet will elevate serum eicosapentaenoic acid (epa) and docosahexaenoic acid (dha) levels and reduce cvd risk markers in dyslipidemic adult men, comparable with the effects generally observed by inclu ... | 2010 | 20797476 |
| integrated biological and chemical analysis of organochlorine compound pollution and of its biological effects in a riverine system downstream the discharge point. | pollution in riverine systems, along with its biological effects, may propagate downstream even at considerable distances. we analyzed the organochlorine compound (oc) pollution in a section of the low ebro river (northeast spain) downstream a long-operating chlor-alkali plant. maximal levels of ocs and of their associated dioxin-like biological activity occurred in residue samples from the plant, and persisted in river sediments some 40km downstream (xerta site). biological analysis at multiple ... | 2010 | 20800876 |
| fetuin-a and cystatin c are endogenous inhibitors of human meprin metalloproteases. | meprin α and β, zinc metalloproteinases, play significant roles in inflammation, including inflammatory bowel disease (ibd), possibly by activating cytokines, like interleukin 1β, interleukin 18, or tumor growth factor α. although a number of potential activators for meprins are known, no endogenous inhibitors have been identified. in this work, we analyzed the inhibitory potential of human plasma and identified bovine fetuin-a as an endogenous meprin inhibitor with a k(i) (inhibition constant) ... | 2010 | 20806899 |
| cell cycle and apoptosis regulatory protein (carp)-1 is a novel, adriamycin-inducible, diffuse large b-cell lymphoma (dlbl) growth suppressor. | diffuse large b-cell lymphoma (dlcl) accounts for 30-40% of adult non-hodgkin's lymphoma (nhl). current anti-nhl therapies often target cellular growth suppression pathways and include r-chop (cyclophosphamide, adriamycin, vincristine, and prednisone plus monoclonal anti-cd20 antibody rituximab). however, since many patients relapse, resistant cells to these therapies remain a significant problem and necessitate development of new intervention strategies. cell cycle and apoptosis regulatory prot ... | 2010 | 20809119 |
| influence of a toxic microcystis aeruginosa strain on glutathione synthesis and glutathione-s-transferase activity in common carp cyprinus carpio (teleostei: cyprinidae). | we evaluated the effects of aqueous extracts of the cyanobacterium-producing microcystin (mc), microcystis aeruginosa (strain rst9501), on detoxification capacity and glutathione (gsh) synthesis in liver, brain, gill, and muscle-as well as apoptotic protease (calpain) activity in liver and brain-in the common carp cyprinus carpio (teleostei: cyprinidae). experimental groups were defined as follows: (1) control (ctr); (2) carp treated with an aqueous extract from the toxic cyanobacteria m. aerugi ... | 2011 | 20809345 |
| oxidative stress biomarkers in cyprinus carpio exposed to commercial herbicide bispyribac-sodium. | cyprinus carpio were exposed under field conditions to 20.87 microg l(-1) of commercial herbicide bispyribac-sodium (nominee, sc), during 7, 21 and 72 days. enzymatic parameters such as catalase (cat), glutathione s-transferase (gst) and acetylcholinesterase (ache) activities, as well as thiobarbituric acid-reactive substances (tbars) and protein carbonyl contents were studied in different tissues. after 7 days of exposure, gst activity decreased. at the same period, brain ache activity increase ... | 2010 | 20809548 |
| toxicity of organic pollutants to seven aquatic organisms: effect of polarity and ionization. | the toxicity of organic chemicals to vibrio fischeri, river bacteria, algae, daphnia magna and fishes were analysed. the results showed that the toxicity of chemicals to narcotics was dependent on hydrophobicity. a single model for both polar and non-polar narcotics was developed by inclusion of a polarity descriptor as well as the hydrophobic parameter. the highly hydrophobic polar narcotics could be treated as non-polar narcotics because their polar functional group(s) make(s) a relatively sma ... | 2010 | 20818578 |
| immunomodulatory effect of aegle marmelos leaf extract on freshwater fish cyprinus carpio infected by bacterial pathogen aeromonas hydrophila. | aquatic organisms (especially fish) require potent defense mechanisms to protect themselves against pathogen invasion and disease formation. the use of immunostimulants in fish culture can prevent the diseases through augmentation of both specific and non-specific immunity. | 2010 | 20818950 |
| carbonic anhydrase related protein viii and its role in neurodegeneration and cancer. | mammalian carbonic anhydrase (α-ca) gene family comprises sixteen isoforms, thirteen of which are active isozymes and three isoforms lack classical ca activity of reversible hydration of co(2) due to absence of one or more histidine residues required for ca catalytic activity. the inactive isoforms are known as carbonic anhydrase related proteins (carps) viii, x and xi. among these three, carp viii was reported first in 1990 from a mouse brain cdna library and is well studied structurally as wel ... | 2010 | 20819067 |
| hemolysin etha in edwardsiella tarda is essential for fish invasion in vivo and in vitro and regulated by two-component system esra-esrb and nucleoid protein hhaet. | edwardsiella tarda is a gram-negative pathogen for hemorrhagic septicemia in fish. recently, two-component system (tcs) esra-esrb in e. tarda has been found to play key roles in regulating type iii secretion system (ttss) and type vi secretion system (t6ss). in this study, a markedly attenuated δesrb mutant was investigated to exhibit enhanced cell-invasion capability, as well as the increased cytotoxicity of its extracellular products (ecps). compared with the parental strain, the δesrb mutant ... | 2010 | 20832475 |
| variability of responses in the crucian carp carassius carassius from two ukrainian ponds determined by multi-marker approach. | the aim of this study was to evaluate the health status of the comparatively tolerant fish carassius carassius over three seasons in an area characterized by spontaneous human activities. sites near the springs of a river (site z) and downstream of a river (site b) in western ukraine were selected. according to the centroid grouping analysis, the biochemical and morphological indices allowed the fish to be distinguished according to season more than to site. the level of nuclear abnormalities wa ... | 2010 | 20832861 |
| ultrastructural aspects of spermatogenesis, testes, and vas deferens in the parthenogenetic tapeworm atractolytocestus huronensis anthony, 1958 (cestoda: caryophyllidea), a carp parasite from slovakia. | spermatogenesis, testes, and vas deferens in the parthenogenetic monozoic tapeworm atractolytocestus huronensis anthony, 1958 (cestoda: caryophyllidea) from slovakia, parasitizing the carp cyprinus carpio l., have been investigated by means of transmission electron microscopy for the first time. the present results show that helminths with parthenogenetic and normal reproduction may share some common spermatology features, e.g., dense cytoplasm of the peripherally localized spermatogonia or a ro ... | 2011 | 20838810 |
| a new pathway encompassing calpain 3 and its newly identified substrate cardiac ankyrin repeat protein is involved in the regulation of the nuclear factor-κb pathway in skeletal muscle. | a multiprotein complex encompassing a transcription regulator, cardiac ankyrin repeat protein (carp), and the calpain 3 protease was identified in the n2a elastic region of the giant sarcomeric protein titin. the present study aimed to investigate the function(s) of this complex in the skeletal muscle. we demonstrate that carp subcellular localization is controlled by the activity of calpain 3: the higher the calpain 3, the more important the sarcomeric retention of carp. this regulation would o ... | 2010 | 20860623 |
| cxcl8 chemokines in teleost fish: two lineages with distinct expression profiles during early phases of inflammation. | during the inflammatory process, chemokine cxcl8 plays a pivotal role in recruitment of human neutrophilic granulocytes. a diversity of sequences similar to cxcl8 was reported in fish, but their evolutionary relationships and functional homology with their human homolog remain unclear. | 2010 | 20865040 |
| a novel (18)o inverse labeling-based workflow for accurate bottom-up mass spectrometry quantification of proteins separated by gel electrophoresis. | in the present work we report on a novel and fast protocol for accurate bottom-up protein quantification that overcomes the drawbacks of in-gel digestion and maldi analysis, while maintaining their benefits. it relies on the following steps: (i) gel electrophoresis separation of proteins, (ii) fast in-gel protein digestion with trypsin, (iii) (18)o-labeling through the decoupled method, (iv) quantification through selected peptides previously chosen using the (18)o inverse labeling approach and ... | 2010 | 20882554 |
| trypanosomiasis-induced th17-like immune responses in carp. | in mammalian vertebrates, the cytokine interleukin (il)-12 consists of a heterodimer between p35 and p40 subunits whereas interleukin-23 is formed by a heterodimer between p19 and p40 subunits. during an immune response, the balance between il-12 and il-23 can depend on the nature of the pathogen associated molecular pattern (pamp) recognized by, for example tlr2, leading to a preferential production of il-23. il-23 production promotes a th17-mediated immune response characterized by the product ... | 2010 | 20885956 |
| mass mortality associated with koi herpesvirus in wild common carp in canada. | koi herpesvirus (khv) was identified as being associated with more than one mortality event affecting common carp in canada. the first was an extensive mortality event that occurred in 2007 in the kawartha lakes region, ontario, affecting lakes scugog and pigeon. fish had branchial necrosis and hepatic vasculitis with an equivocal interstitial nephritis. several fish also had branchial columnaris. subsequent mortality events occurred in 2008 in additional bodies of water in south-central ontario ... | 2010 | 20966274 |
| the tissue factor pathway inhibitor 1 of sciaenops ocellatus possesses antimicrobial activity and is involved in the immune response against bacterial infection. | tissue factor pathway inhibitor 1 (tfpi-1) is a kunitz-type serine protease inhibitor that regulates the activation of tissue factor-induced coagulation. in teleosts, tfpi-1-like sequences have been found to exist in two species (danio rerio and cyprinus carpio); however, the potential function of fish tfpi-1 has not been investigated. in this study, we identified and analyzed a tfpi-1 homologue, sotfpi-1, from red drum (sciaenops ocellatus). the deduced amino acid sequence of sotfpi-1 is 284 re ... | 2010 | 20970444 |
| prevalence and sequence comparison of phyllodistomum folium from zebra mussel and from freshwater fish in the ebro river. | we utilised dna analysis to detect the presence of the digenean phyllodistomum folium in three cyprinid species, scardinius erythrophthalmus, cyprinus carpio and rutilus rutilus. dna sequencing of the region containing the genes its1-5.8s-its2 revealed 100% sequence identity between dna from the sporocysts found in zebra mussels and dna from adults located in the urinary system of 29 cyprinid fish. a second genetically different (variation=1.6%) sequence was observed in two samples from r. rutil ... | 2010 | 20971214 |
| derivation and characterization of embryonic stem-like cells of indian major carp catla catla. | embryonic stem (es)-like cells were derived from mid-blastula stage embryos of a freshwater fish, catla catla catla, under feeder-free condition and designated as cces cells. the conditioned media was optimized with 10% foetal bovine serum (fbs), fish embryo extract (fee) having 100 µg ml(-1) protein concentration, 15 ng ml(-1) basic fibroblast growth factor (bfgf) and basic media containing leibovitz-15, dmem with 4·5 g l(-1) glucose and ham's f12 (ldf) in 2:1:1 ratio using a primary culture of ... | 2010 | 21039493 |
| identification of calcium-binding proteins in fish seminal plasma. | calcium ions play an important role in the activation of fish sperm movement. the mechanism of their binding in semen is still unknown. the goal of this study was the development of a method for identifying calcium-binding proteins in fish seminal plasma. two methods of calcium-binding proteins detection were tested with the use of quin2 and stains-all dyes. the first method was useful for the identification of calcium-binding proteins of fish seminal plasma. it consisted of proteins separation ... | 2010 | 21042848 |
| expression of immune-related genes in rohu labeo rohita (hamilton) by experimental freshwater lice argulus siamensis (wilson) infection. | the crustacean ectoparasite, argulus poses one of the major threats to carp culture due to absence of any suitable control measure. the study was undertaken to determine the expression of immune-related genes in three major immunocompetent organs viz., kidney, skin and liver of rohu (labeo rohita) during experimental freshwater lice argulus siamensis infection. results showed that the expression of tlr 22-like, lysozyme g and β2-microglobulin genes in kidney was significantly (p ≤ 0.05) down-reg ... | 2010 | 21051150 |
| biologic responses of bacteria communities living at the mucus secretion of common carp (cyprinus carpio) after exposure to the carbon nanomaterial fullerene (c(60)). | bacteria communities living in mucus secretions of common carp cyprinus carpio (cyprinidae) were exposed to the organic nanomaterial fullerene (c(60)) to evaluate its potential bactericidal effects. end points analyzed were viability, growth, reactive oxygen species (ros) concentration, and total antioxidant competence against peroxyl radicals. viability was not affected (p > 0.05), whereas growth was arrested (p < 0.05) after 3 hours of exposure to the three concentration of c(60) assayed (0.1, ... | 2010 | 21072630 |
| toxicological responses of cyprinus carpio after exposure to a commercial herbicide containing imazethapyr and imazapic. | cyprinus carpio was exposed to imazethapyr and imazapic at laboratory and at field conditions. the laboratory experiment was carried out for 7 days and at rice field for 7, 30 and 90 days. oxidative stress parameters and antioxidant profile were studied as well as metabolic parameters. after 7 days, brain ache activity increases in laboratory and field, but in muscle, reduction was observed only in laboratory. at the same period, brain and muscle tbars and liver cat increase in the laboratory. m ... | 2010 | 21095003 |
| the rapid generation of chimerical genes expanding protein diversity in zebrafish. | variation of gene number among species indicates that there is a general process of new gene origination. one of the major mechanism providing raw materials for the origin of new genes is gene duplication. retroposition, as a special type of gene duplication- the rna-based duplication, has been found to play an important role in new gene evolution in mammals and plants, but little is known about the process in the teleostei genome. | 2010 | 21106061 |
| differential responses to natural and recombinant allergens in a murine model of fish allergy. | aerosolized fish proteins are an important cause of allergic airway reactions in both the domestic and the occupational environment. the aim of this study was to investigate inhalant fish-induced allergy in a mouse model and compare immune responses generated by raw and heat-treated fish extracts as well as natural and recombinant forms of the major fish allergen parvalbumin. mice were sensitized with raw or cooked pilchard extract and challenged intranasally with cooked pilchard extract, purifi ... | 2010 | 21112638 |
| cyprinid herpesvirus 3. | the recently designated cyprinid herpesvirus 3 (cyhv-3) is an emerging agent that causes fatal disease in common and koi carp. since its emergence in the late 1990s, this highly contagious pathogen has caused severe financial losses in common and koi carp culture industries worldwide. in addition to its economic role, recent studies suggest that cyhv-3 may have a role in fundamental research. cyhv-3 has the largest genome among viruses in the order herpesvirales and serves as a model for mutagen ... | 2010 | 21122210 |
| hippocampal carp over-expression solidifies consolidation of contextual fear memories. | the doublecortin-like kinase (dclk) gene is involved in neuronal migration during development. through alternative splicing the dclk gene also produces a transcript called ca(2+)/calmodulin dependent protein kinase (camk)-related peptide (carp) that is expressed exclusively during adulthood in response to neuronal activity. the function of carp, however, is poorly understood. to study carp function, we have generated transgenic mice with over-expression of the carp transcript in, amongst other b ... | 2010 | 21130104 |
| activity and function of rabbit muscle-specific creatine kinase at low temperature by mutation at gly268 to asn268. | carp muscle-specific creatine kinase m1 isoenzyme (m1-ck) seems to have evolved to adapt to synchronized changes in body temperature and intracellular ph. when gly(268) in rabbit muscle-specific creatine kinase was substituted with asn(268) as found in carp m1-ck, the rabbit muscle-specific ck g286n mutant specific activity at ph 8.0 and 10°c was more than 2-fold higher than that in the wild-type rabbit enzyme. kinetic studies showed that k(m) values of the rabbit ck g268n mutant were similar to ... | 2010 | 21130895 |
| the ontogeny of mauthner cells in the brain of labeo rohita as revealed by nadph-d and nnos immunohistochemistry. | nitric oxide (no) is well demonstrated to act as a neuronal messenger in neurotransmission in vertebrate animals. we are for the first time reporting nicotinamide adenine dinucleotide phosphate-diaphorase (nadph-d) and neuronal nitric oxide synthase (nnos) in the mauthner cells (mc) of the carp, labeo rohita brain during post-embryonic development. the nadph-d and nnos-positive mc were seen intensely labeled in all young stages on either side of the ventricle in the hindbrain. in adult, these ce ... | 2010 | 21132510 |
| the application of hcg, cph and ovopel in successful artificial reproduction of goldfish (carassius auratus auratus) under controlled conditions. | artificial reproduction of fish is one of the main goals of aquaculture production. the aim of this study is to optimize the method of goldfish reproduction under controlled conditions by comparing the effectiveness of carp pituitary homogenate (cph), ovopel and human chorionic gonadotropin (hcg), administered as a one-off dose and inducing two spawns in the same fish within a short time period. goldfish spawners were stimulated with hcg, cph and ovopel, and the results were compared to the fish ... | 2010 | 21134006 |
| generation of monoclonal antibodies specific for orf68 of koi herpesvirus. | outbreaks of koi herpesvirus (khv) infection in carp are still a serious problem worldwide. khv is closely related to other two cyprinid herpesviruses, pox herpesvirus (chv) and haematopoietic necrosis herpesvirus (cyhv-2) in goldfish. in this study, two major khv antigenic proteins (orf62 and orf68) were identified by immunoscreening using a khv-specific polyclonal antibody, and then monoclonal antibodies were generated for immunodiagnostic studies. after screening hybridoma cells, one mab agai ... | 2010 | 21134694 |
| visualizing a set of olfactory sensory neurons responding to a bile salt. | in the present study, we exposed the olfactory epithelia of crucian carp, carassius carassius, and brown trout, salmo trutta, to dextran coupled with alexa dyes together with odorants. dye uptake was severely reduced after pre-exposure to nocodazole, an inhibitor of microtubule polymerization that impairs endocytosis, supporting the hypothesis that odour-activated olfactory receptor molecules undergo endocytosis. application of the bile acid taurolithocholate, a potent and specific odorant for f ... | 2011 | 21147971 |
| the individual and combined effects of deoxynivalenol and aflatoxin b(1) on primary hepatocytes of cyprinus carpio. | aflatoxin b(1) (afb(1)) and deoxynivalenol (don) are important food-borne mycotoxins that have been implicated in animal and human health. in this study, individual and combinative effects of afb(1) and don were tested in primary hepatocytes of cyprinus carpio. the results indicated that the combinative effects of afb(1) and don (0.01 μg/ml afb(1) and 0.25 μg/ml don; 0.02 μg/ml afb(1) and 0.25 μg/ml don; 0.02 μg/ml afb(1) and 0.5 μg/ml don) were higher than that of individual mycotoxin (p < 0.05 ... | 2010 | 21152299 |
| isolation and characterization of koi herpesvirus (khv) from indonesia: identification of a new genetic lineage. | koi herpesvirus (khv) is the aetiological agent of an emerging disease (khvd) associated with mass mortalities in koi and common carp and reported from at least 30 countries. we report the first isolation of khv from koi and common carp in indonesia and initial characterization of the isolates. clinical signs, histopathology and virion morphology are similar to those of isolates from other countries. phylogenetic analyses using the thymidine kinase gene amplified from each isolate and from carp ... | 2010 | 21158870 |
| haematological indices are modulated in juvenile carp, cyprinus carpio l., exposed to microcystins produced by cyanobacterial water bloom. | this study evaluated the influence of toxic cyanobacterial water blooms on the blood indices of the common carp, cyprinus carpio l. experimental fish were exposed to a natural population of cyanobacterial water blooms (mainly microcystis aeruginosa and m. ichthyoblabe), which contained microcystins [total concentration 133-284 μg g⁻¹ (dw), concentration in water 2.8-7.4 μg l⁻¹]. haematological indices showed marked changes in fish exposed to the cyanobacterial population in comparison with the c ... | 2010 | 21158871 |
| potential use of probiotic- and triherbal extract-enriched diets to control aeromonas hydrophila infection in carp. | this study reports the effect of probiotic- and triherbal extract-enriched diet on cyprinus carpio (52 +/- 2 g, n = 250) inoculated intramuscularly (50 microl) with aeromonas hydrophila (1.8 x 106 cfu ml(-1)). on day 6 post-infection, the fish were divided into 4 groups and fed with the basal diet (iu) or with diets supplemented with triherbal extract (h), sporolac (s) or lactobacillus (l). a fifth group of non-infected fish fed with the basal diet was used as a control. haematology and immunolo ... | 2010 | 21166313 |
| [correlation and location of est markers with cold tolerance trait of common carp (cyprinus carpio l)]. | expressed sequence tags (ests) are parts of complementary dnas (cdnas) with certain gene function.it provides us more information than other neutral markers. the association of est markers with phenotypes can increase our understanding of the biochemical pathways and mechanisms affecting economically important traits. in this study, 12 candidate est markers isolated from the cold-induced brain cdna of common carp (cyprinus carpio l.) were conducted the correlation analysis of marker and cold tol ... | 2010 | 21174346 |
| nitrosative stress during infection-induced inflammation in fish: lessons from a host-parasite infection model. | the inflammatory response should be considered a protective immune reaction of the host aimed at the removal of pathogens, sometimes irrespective of negative side-effects. in this review we discuss the differential contribution of macrophages and neutrophilic granulocytes to nitrosative stress in vivo and discuss how the timing and concentration of nitric oxide (no·) are important factors determining the degree of nitrosative stress during parasite-induced inflammation. infections of common carp ... | 2010 | 21184662 |
| genome of turbot rhabdovirus exhibits unusual non-coding regions and an additional orf that could be expressed in fish cell. | genomic sequence of scophthalmus maximus rhabdovirus (smrv) isolated from diseased turbot has been characterized. the complete genome of smrv comprises 11,492 nucleotides and encodes five typical rhabdovirus genes n, p, m, g and l. in addition, two open reading frames (orf) are predicted overlapping with p gene, one upstream of p and smaller than p (temporarily called ps), and another in p gene which may encodes a protein similar to the vesicular stomatitis virus c protein. the c orf is containe ... | 2010 | 21185339 |
| indian carp (labeo rohita) gall bladder poisoning-report of four cases in a single family. | the ingestion of indian carp gallbladder may result in transient hepatitis with subsequent acute renal failure. this case series also illustrates the importance of understanding the use and potential serious complications of alternative medicines. so fish gallbladder poisoning should be considered in unexplained acute renal failure in chinese and asian patients. we report four family members who developed acute renal failure and toxic hepatitis at the same time following ingestion of raw indian ... | 2011 | 21207198 |
| population study of atractolytocestus huronensis (cestoda: caryophyllidea), an invasive parasite of common carp introduced to europe: mitochondrial cox1 haplotypes and intragenomic ribosomal its2 variants. | the invasive monozoic tapeworm atractolytocestus huronensis, a specific parasite of common carp, was originally found and described in the north american continent. it has been introduced to europe and reported in several countries in the last 15 years, as well. in the current study, tapeworms from one north american (usa) and five european localities (united kingdom/uk, slovakia, hungary, croatia, and romania) were subjected to molecular analyses in order to determine the level of intrapopulati ... | 2011 | 21210151 |
| identification of genes that elicit disuse muscle atrophy via the transcription factors p50 and bcl-3. | skeletal muscle atrophy is a debilitating condition associated with weakness, fatigue, and reduced functional capacity. nuclear factor-kappab (nf-κb) transcription factors play a critical role in atrophy. knockout of genes encoding p50 or the nf-κb co-transactivator, bcl-3, abolish disuse atrophy and thus they are nf-κb factors required for disuse atrophy. we do not know however, the genes targeted by nf-κb that produce the atrophied phenotype. here we identify the genes required to produce disu ... | 2011 | 21249144 |
| comparative susceptibility of carp fingerlings to lernaea cyprinacea infection. | study was conducted to find out the comparative susceptibility of fingerlings of seven species of carps (labeo fimbriatus, l. rohita, l. calbasu, catla catla, ctenopharyngodon idella, cyprinus carpio and hypophthamichthys molitrix) grown under both mono and polyculture to lernaea cyprinacea infection. under monoculture, c. carpio, l. rohita and l. calbasu, did not acquire lernaea infection and were thus considered resistant, whereas c. idella, h. molitrix, c. catla and l. fimbriatus were suscept ... | 2010 | 21255932 |
| conservation of characteristics and functions of cd4 positive lymphocytes in a teleost fish. | the presence of helper and cytotoxic t cells in fish has been suggested, although t cell subsets have yet to be identified at the cellular level. in order to investigate the functions of cd4 and cd8a positive t cells we attempted to produce and characterize monoclonal antibodies (mabs) against teleost cd4 and cd8a. here we report the successful production of mabs against cd4 and cd8a in clonal ginbuna crucian carp carassius auratus langsdorfii and the function of cd4 positive t cells. in this st ... | 2011 | 21272597 |
| novel recombinant monoclonal antibodies for vitellogenin assays in cyprinid fish species. | various polyclonal and monoclonal antibodies have been developed for vitellogenin (vtg) bioassays in different aquatic species. preparation of these reagents is time-consuming and expensive. in the present study, a phage-displayed, recombinant, single-chain variable fragment (scfv) format antibody library was constructed using splenic mrna from non-immunized mice. after 3 rounds of panning, 3 scfv antibodies with specificity for the highly conserved n-terminal region of cyprinid fish vtg were is ... | 2010 | 21290899 |
| immunostimulatory activities of specific bacterial secondary metabolite of anoxybacillus flavithermus strain sx-4 on carp, cyprinus carpio. | aims: to determine the capacity of secondary metabolite of strain sx-4, to enhance the nonspecific immunity and survival of carp (cyprinus carpio), and to identify the constituents that are responsible. methods and results: a thermophilic strain sx-4 that is able to produce immunostimulatory metabolite was isolated from sludge sample of hot spring and identified by comparison with 16s rrna sequences (99% of homology) as anoxybacillus flavithermus. bioactivity-guided fractionation of methanol e ... | 2011 | 21294820 |
| a novel soluble immune-type receptor (sitr) in teleost fish: carp sitr is involved in the nitric oxide-mediated response to a protozoan parasite. | the innate immune system relies upon a wide range of germ-line encoded receptors including a large number of immunoglobulin superfamily (igsf) receptors. different ig-like immune receptor families have been reported in mammals, birds, amphibians and fish. most innate immune receptors of the igsf are type i transmembrane proteins containing one or more extracellular ig-like domains and their regulation of effector functions is mediated intracellularly by distinct stimulatory or inhibitory pathway ... | 2011 | 21305002 |
| destabilization of carp mrnas by aloe-emodin contributes to caspase-8-mediated p53-independent apoptosis of human carcinoma cells. | using short hairpin rna against p53, transient ectopic expression of wild-type p53 or mutant p53 (r248w or r175h), and a p53- and p21-dependent luciferase reporter assay, we demonstrated that growth arrest and apoptosis of fadu (human pharyngeal squamous cell carcinoma), hep3b (hepatoma), and mg-63 (osteosarcoma) cells induced by aloe-emodin (ae) are p53-independent. co-immunoprecipitation and small interfering rna (sirna) studies demonstrated that ae caused s-phase cell cycle arrest by inducing ... | 2011 | 21308745 |
| a comparative review on european-farmed finfish rna viruses and their vaccines. | the diseases causing the highest ecological and socio-economical impacts in european farmed finfish are produced by rna viruses. salmon, trout, sea bream, sea bass, carp and turbot, suffer viral nervous necrosis produced by betanodaviruses (vnnv), infectious pancreatic necrosis produced by aquabirnaviruses (ipnv), viral haemorrhagic septicemia (vhsv) and infectious haematopoietic necrosis (ihnv) produced by novirhabdoviruses, spring viremia of carp produced by vesicular-like rhabdoviruses (svcv) ... | 2011 | 21320546 |
| immunostimulatory activities of a decapeptide derived from alcaligenes faecalis fy-3 to crucian carp. | a strain was isolated from a soil sample collected from weihe river in shaanxi province (108°03'e 34°14'n), which was identified as alcaligenes faecalis by 16s rrna analysis. a compound m showing potent immune activity was isolated from secondary metabolites of the strain through bioassay-guided isolation techniques. the structure of the compound m was elucidated using ft-ir, ei-ms, 1h nmr and 13c nmr spectra and identified as cyclo-(l-pro-gly)5 which was first time reported as a natural product ... | 2011 | 21332568 |
| [study on genotoxicity of aldicarb and methomyl]. | genotoxicity of aldicarb and methomyl was explored. the aldicarb and methomyl were diluted by the deionized water respectively, and then five concentrations of aldicarb were generated as 0.002, 0.02, 0.2, 2, 20 microg/l, methomyl as 0.02, 0.2, 2, 20, 200 microg/l. the micronucleus of carp erythrocyte was counted by micronucleus test. the mutation of bacteria was assessed by ames test. the dna damage of human lymphocytes was tested by comet assay. the genotoxicity of aldicarb and methomyl was est ... | 2010 | 21360888 |
| reduction of stress response in carp,cyprinus carpiol., held under deteriorating environmental conditions, by oral administration of bovine lactoferrin. | bovine lactoferrin (lf) was evaluated for its ability to suppress stress reactions in carp, cyprinus carpio l., held under deteriorating environmental conditions. three test diets containing different levels of lf (0.01, 0.1 and 1%) were fed at 2% of fish body weight per day, i.e. lf at 2, 20 and 200 mg kg(-1) body weight day(-1) was administered orally to carp for 14 days at 20 °c. in the fish reared at a density of 2 individuals 10 l(-1) , plasma cortisol, adrenaline and noradrenaline decrease ... | 1998 | 21361970 |
| short communicationgill lesions in common carp, cyprinus carpio l., in mexico due to the metacercariae of centrocestus formosanus. | | 1998 | 21361978 |
| specific and nonspecific immunostimulation study of euphorbia hirta on pseudomonas fluorescens-infected cyprinus carpio. | infectious diseases are one of the major factors affecting the production of fish worldwide. the pathogens (especially bacteria) affect the immune system of fish and the administration of immunostimulants can increase resistance to infectious diseases by enhancing both specific and nonspecific defense mechanisms. | 2011 | 21385104 |
| generation and characterization of koi herpesvirus recombinants lacking viral enzymes of nucleotide metabolism. | koi herpesvirus (khv) causes a fatal disease in koi and common carp, but no reliable and genetically characterized vaccines are available up to now. therefore, we generated khv recombinants possessing deletions within the viral ribonucleotide reductase (rnr), thymidine kinase (tk), dutpase, or tk and dutpase genes, and their corresponding rescuants. all khv mutants were replication competent in cultured cells. whereas plaque sizes and titers of rnr-negative khv were reduced, replication of the o ... | 2011 | 21387205 |
| application of sensory and microbial analysis to assess quality of fish in siliguri city of west bengal, india. | sensory and certain microbial analyses were applied to assess the quality of raw fish sold at a market in siliguri cityof west bengal, india. in regular surveys undertaken during june to august 2008, a particular fish species was randomly selected, its source was noted and a sensory analysis, the quality index method (qim) was applied to assess its quality raw fish samples were also collected and a small quantity (about 1 g) of scales oran upper layer of the skin surface (forscale-less fish samp ... | 2010 | 21387907 |
| investigation of cyprinid herpesvirus-3 genetic diversity by a multi-locus variable number of tandem repeats analysis. | cyprinid herpesvirus-3 (cyhv-3), or koi herpesvirus (khv), is responsible for high mortalities in aquaculture of both common carp (cyprinus carpio carpio) and koi carp (cyprinus carpio koi) worldwide. the complete genomes of three cyhv-3 isolates showed more than 99% of dna sequence identity, with the majority of differences located in short tandem repeats, also called vntr (variable number of tandem repeats). by targeting these variations, eight loci were selected for genotyping cyhv-3 by multi ... | 2011 | 21392536 |
| mechanism of blood coagulation in common carp (cyprinus carpio). | in vitro, carp blood was anticoagulated by using mgso(4) at a final concentration of 22.2 mmol l(-1) and sodium citrate at a final concentration of 11.8 mmol l(-1) . the coagulation times for carp plasma diluted by ion-free water (1:1), and that of carp plasma to which thrombocytes and small lymphocytes were added, were measured at 23 °c using standard methods, and then contrasted with the coagulation times for plasma obtained from chickens and rabbits. the shapes of the thrombocytes and small l ... | 2006 | 21396003 |
| toll-like receptors in bony fish: from genomics to function. | receptors that recognize conserved pathogen molecules are the first line of cellular innate immunity defense. toll-like receptors (tlrs) are the best understood of the innate immune receptors that detect infections in mammals. key features of the fish tlrs and the factors involved in their signaling cascade have high structural similarity to the mammalian tlr system. however, the fish tlrs also exhibit very distinct features and large diversity which is likely derived from their diverse evolutio ... | 2011 | 21414346 |
| immunomodulatory effects of secondary metabolites from thermophilic anoxybacillus kamchatkensis xa-1 on carp, cyprinus carpio. | a bacterial strain with putative immunomodulatory properties was isolated from xi'an hot springs in china. comparison of 16s rrna gene revealed a 97% similarity between the tested strain (designated xa-1) and anoxybacillus kamchatkensis. two compounds isolated from the secondary metabolites of xa-1 were identified by spectral data (infrared, nuclear magnetic resonance and mass spectrometry) as: (1) cyclo (gly-l-pro) and (2) cyclo (l-ala-4-hydroxyl-l-pro). two cyclic dipeptides showed stimulatory ... | 2011 | 21421057 |
| antioxidative effects of n-acetylcysteine, lipoic acid, taurine, and curcumin in the muscle of cyprinus carpio l. exposed to cadmium. | we investigated the muscle tissue of a teleost cyprinus carpio l. to find out whether n-acetylcysteine (nac), alpha-lipoic acid (la), taurine (tau), and curcumin (cur) were able to counteract oxidative stress induced by acute exposure to cadmium (cd). the muscle tissue was dissected 96 h after a single intraperitoneal injection of cd (5 mg kg(-1)) and of antioxidant substances (50 mg kg(-1)). using spectrophotometry, we determined the glutathione redox status, lipid peroxidation levels and the a ... | 2011 | 21421527 |
| time-dependent oxidative stress and histopathological changes in cyprinus carpio l. exposed to microcystin-lr. | microcystins (mcs) are produced by cyanobacteria in aquatic environments and are a potential risk to aquatic organisms. increasing evidence suggests that oxidative stress may play an important role in the toxicity mechanism of mcs on fish, but most studies were based on relatively high concentrations. in this study, the effect of time-dependent oxidative stress in livers of cyprinus carpio l. (c. carpio) exposed to 10 µg l(-1) of microcystin-lr (mc-lr) for 0-14 days was investigated. mc-lr induc ... | 2011 | 21455607 |
| candidate genes in patients with autoinflammatory syndrome resembling tumor necrosis factor receptor-associated periodic syndrome without mutations in the tnfrsf1a gene. | tumor necrosis factor (tnf) receptor-associated periodic syndrome (traps) is an autosomal-dominant multisystemic autoinflammatory condition. patients display different mutations of the tnf receptor superfamily 1a gene (tnfrsf1a), coding for a nearly ubiquitous tnf receptor (tnfr1). no tnfrsf1a mutation has been identified in a proportion of patients with traps-like phenotype. | 2011 | 21459945 |
| water quality evaluation of two interconnected dam lakes with field-captured and laboratory-acclimated fish, cyprinus carpio. | karakaya and sultansuyu dam lakes, located in the eastern part of turkey, are important water sources, both for irrigation and fishery. the main goal of the study was to investigate water qualities of dam lakes using a set of biomarkers in the fish cyprinus carpio. for this aim, field sample and laboratory-acclimated fish were compared to identify changes in selected biomarkers. the activities of ethoxyresorufin-o-deethylase, glutathione s-transferase, glutathione reductase, and carboxylesterase ... | 2011 | 21461606 |
| genotoxicity of crude extracts of cyanobacteria from taihu lake on carp (cyprinus carpio). | genotoxicity of crude cyanobacteria extracts (cbe) from blooms in taihu lake, china toward common carp (cyprinus carpio) was measured. the primary extracellular product was determined by hplc to be microcystin-lr (mc-lr, l for leucine and r for arginine) with an average concentration of 2.4 × 10(2) μg mc g(-1) dry weight of cyanobacteria. acute toxicity to carp, expressed as the 72-h lc(50,) was 53 mg, dw cyanobacteria l(-1). genotoxicity, as determined by the micronucleus (mn) and comet assays ... | 2011 | 21472455 |
| recombinant outer membrane protein a (ompa) of edwardsiella tarda, a potential vaccine candidate for fish, common carp. | outer membrane protein a (ompa) is a component of the outer membrane of edwardsiella tarda and is wildly distributed in enterobacteriaceae family. the gene encoding the ompa protein was cloned from e. tarda and expressed in escherichia coli m15 cells. the recombinant ompa protein containing his(6) residues was estimated to have a molecular weight of ∼38kda. in western blot the native protein showed expression at ∼36kda molecular weight which was within the range of major outer membrane proteins ... | 2011 | 21482086 |
| bioaccumulation of microcystins by fish associated with a persistent cyanobacterial bloom in lago de patzcuaro (michoacan, mexico). | lago de patzcuaro is a historically important freshwater fishery in mexico. the lake is presently characterized by a persistent bloom of cyanobacteria, specifically dominated by recognized producers of toxic microcystins (mcysts). we evaluated mcysts in sestonic and dissolved fractions of the water column, as well as representative fish species (silversides, chirostoma spp.; goodea sp.; and carp, cyprinus carpio) obtained from local markets and small commercial catches during the bloom. samples ... | 2011 | 21495029 |
| isolation of enriched carp spermatogonial stem cells from labeo rohita testis for in vitro propagation. | the in vitro culture system for spermatogonial stem cells (sscs) is a powerful tool for exploring molecular mechanisms of male gametogenesis and gene manipulation. very little information is available for fish ssc biology. our aim was to isolate highly pure sscs from the testis of commercially important farmed carp, labeo rohita. the minced testis of l. rohita was dissociated with collagenase. dissociated cells purified by two-step ficoll gradient centrifugation followed by magnetic activated ce ... | 2011 | 21496900 |
| molecular characterization and different expression patterns of the muscle ankyrin repeat protein (marp) family during porcine skeletal muscle development in vitro and in vivo. | carp, ankrd2, and darp belong to the ankyrin repeat protein (marp) family and play a critical role in the integration of cytoskeletal architecture, stress response, and transcriptional regulation. in this study, we cloned the cdna and promoter sequences of porcine ankyrin repeat protein (marp) gene family. rt-pcr analysis revealed that porcine carp gene was predominantly expressed in heart. ankrd2 was widely expressed in many tissues, a high expression level was observed in the skeletal muscle a ... | 2011 | 21500110 |
| immunomodulating potency of lipopolysaccharides (lps) derived from smooth type of bacterial pathogens in indian major carp. | lipopolysaccharides (lps), the outer membrane of the gram-negative bacteria, are reported to stimulate the immunity of different vertebrates including fish. however, their potency and spectrum of actions often differ among different bacteria. in this study, effect of crude lps, derived from three species of smooth gram-negative bacterial fish pathogens viz. edwardsiella tarda, escherichia coli, and pseudomonas fluorescens, on certain innate immune parameters of indian major carp, labeo rohita wa ... | 2011 | 21507588 |
| organochlorine pesticide and polychlorinated biphenyls levels in fish and mussel in van region, turkey. | forty-seven fish (24 endemic alburnus tarichi, güldenstädt, 1814; 8 capoeta capoeta, guldenstaedt, 1772; 15 mirror carp cyprinus carpio, l., 1758) and 13 mussel (unio stevenianus, krynicki, 1837) samples, with 10 specimens per sample, were collected from van lake, turkey, and rivers flowing into it. gamma-hch was detected in 21 alburnus tarichi samples (56.57 ng/g ± 22.18 ng/g) and in two capoeta capoeta samples (27.6 ng/g and 36.45 ng/g). beta-hch was detected in 8 alburnus tarichi samples (24. ... | 2011 | 21516453 |
| a new myxosporean species myxobolus sclerii sp. nov. and one known species m. stomum ali et al. 2003 from two indian major carp fishes. | the present communication deals with description of one new species of myxobolus (myxozoa: myxosporea: bivalvulida), m. sclerii sp. nov. infecting eye ball of catla catla (hamilton) and redescription of m. stomum infecting scales of labeo rohita (hamilton), two major carps of kanjali and ropar wetlands respectively. spores of m. sclerii sp. nov. measure 7.9-9.5(8.7 ± 1.13) × 4.3-5.7(5 ± 0.98) μm in size. parietal folds absent. polar capsules two, equal and measuring 4-5.4(4.7 ± 0.98) × 1-2.6(1.8 ... | 2010 | 21526031 |
| observation on prevalence of ectoparasites in carp fingerlings in two districts of west bengal. | the present study has been carried out to find out the prevalence of ectoparasites of carp fingerlings during different months of the year 2008-2009. four groups of ectoparasites viz. myxozoan, ciliophoran, monogenean and crustacean were recorded from 400 fingerlings of rohu (labeo rohita), catla (catla catla), mrigal (cirrhinus mrigala), bata (labeo bata), common carp (cyprinus carpio) and lata (channa punctatus) collected from different ponds of nadia and hooghly district of west bengal from j ... | 2010 | 21526033 |
| development and characterization of two new cell lines from common carp, cyprinus carpio (linn). | two new cell lines (ccf and cch) were established from fin and heart tissues of common carp, cyprinus carpio. the cells were optimally maintained in leibovitz-15 medium supplemented with 10% fetal bovine serum (fbs) and 10 ng/ml of basic fibroblastic growth factor (bfgf). the effects of temperature, concentration of fbs and bfgf on the growth of ccf and cch cells were examined. the temperature ranged from 24 to 32 °c for good growth of the cells. the growth rate of cells was higher in medium con ... | 2011 | 21526264 |
| somatic mutagenesis with a sleeping beauty transposon system leads to solid tumor formation in zebrafish. | large-scale sequencing of human cancer genomes and mouse transposon-induced tumors has identified a vast number of genes mutated in different cancers. one of the outstanding challenges in this field is to determine which genes, when mutated, contribute to cellular transformation and tumor progression. to identify new and conserved genes that drive tumorigenesis we have developed a novel cancer model in a distantly related vertebrate species, the zebrafish, danio rerio. the sleeping beauty (sb) t ... | 2011 | 21533036 |
| the influence of douchi starter cultures on the composition of extractive components, microbiological activity, and sensory properties of fermented fish pastes. | three experiments were conducted to test the hypothesis that douchi cultures could serve as a potential starter for enhancing the quality attributes of fermented silver carp meat. in experiment 1, an active, prefermented douchi culture was incorporated into a fish paste to aid in the fish fermentation (30 d) and facilitate biochemical production of extractive flavor components (prf). in experiment 2, a fully fermented (30 d) douchi was added to a fish paste and the mixture was fermented for 30 d ... | 2011 | 21535644 |
| a protocol for the evaluation of genotoxicity in bile of carp (cyprinus carpio) exposed to lake water treated with different disinfectants. | a sensitive and rapid method to evaluate toxic and genotoxic properties of drinking water supplied from lake trasimeno (umbria, central italy) was worked out analysing bile in cyprinus carpio exposed for 20d to lake water treated with 3 different disinfectants, sodium hypochlorite (naclo), chlorine dioxide (clo(2)) and peracetic acid (paa). fish were sacrificed at 0, 10 and 20d in order to investigate the time course of these endpoints. an aliquot of bile samples was fractionated by adsorption o ... | 2011 | 21546055 |
| a cross-sectional investigation of chronic exposure to microcystin in relationship to childhood liver damage in the three gorges reservoir region, china. | background: microcystin-producing microcystis bloom is a severe water problem in the world. some reports indicate that chronic exposure to microcystin may result in liver damage in adults, but information on effects in children is limited. objective: to investigate the relationship between microcystin exposure and liver damage in children. methods: we measured microcystin concentrations in drinking water and aquatic food (carp and duck) from 2 lakes and 4 wells. participants were 1322 children a ... | 2011 | 21561830 |