| synthesis and biological activity of some di(nitrothienyl)- and di(acetylaminothienyl)sulfones. | some new di(nitrothienyl)- and di(acetylaminothienyl)sulfones were synthesized. compounds (i)-(vi) were active against several gram-positive bacteria, in vitro. di(5-acetylamino-2-thienyl)sulfone (vii) showed a mild antimalarial activity against a drug-sensitive strain of p. berghei in mice. | 1983 | 6360709 |
| malaria vaccine antigen(s): detergent solubilization, partial isolation, and recovery of immunoprotective activity. | plasmodium berghei-infected erythrocytes were solubilized with the nonionic detergent n-octyl glucoside and membrane-containing p. berghei fragments with either n-octyl glucoside or the ionic detergent sodium deoxycholate. unsolubilized material was separated by centrifugation, and the detergent was removed from the respective supernatants by gel filtration. reaggregated components in the respective void volume eluates acted as a specific vaccine in recipient mice, resulting in a dose-related ac ... | 1984 | 6360908 |
| 2-acetylpyridine thiosemicarbazones. 8. derivatives of 1-acetylisoquinoline as potential antimalarial agents. | a series of 1-acetylisoquinoline thiosemicarbazones was prepared in order to evaluate their antimalarial properties. this was achieved by the reaction of 1-acetylisoquinoline with methyl hydrazinecarbodithioate to give methyl 3-[1-(1-isoquinolinyl)ethylidene]hydrazinecarbodithioate (ii). displacement of the s-methyl group from this intermediate by various primary and secondary amines afforded the desired 1-acetylisoquinoline thiosemicarbazones (iii). thiosemicarbazides in which the azomethine mo ... | 1984 | 6361257 |
| 2-acetylpyridine thiosemicarbazones. 9. derivatives of 2-acetylpyridine 1-oxide as potential antimalarial agents. | in view of the antimalarial activity in mice of 2-acetylpyridine thiosemicarbazones, a series of analogous 1-oxides was prepared for evaluation. their synthesis was achieved by the reaction of 2-acetylpyridine 1-oxide with methyl hydrazinecarbodithioate to give methyl 3-[1-(2-pyridinyl 1-oxide)ethylidene]hydrazinecarbodithioate (ii). reaction of the latter intermediate with secondary amines afforded the desired 2-acetylpyridine 1-oxide thiosemicarbazones (iii). reduction of the azomethine linkag ... | 1984 | 6361258 |
| the influence of riboflavin deficiency on plasmodium berghei infection in rats. | two experiments were done in which rats in various stages of riboflavin deficiency were infected with plasmodium berghei. various control groups were included to compare the influence of food restriction on the p. berghei infection with that of riboflavin deficiency, namely, pair-fed (pf), weight-matched (wm) and ad libitum-fed (c-al) control groups. riboflavin deficiency depressed maximum parasite counts by comparison with all control groups and the degree of depression was inversely related to ... | 1983 | 6362121 |
| [plasmodium berghei-strongyloides ratti interaction: experimental modification and mechanism]. | | 1983 | 6362579 |
| plasmodium berghei: physiopathological changes during infections in mice. | using infections of plasmodium berghei in laboratory mice, the following physiopathological changes were observed during the seven days of the infection: reduction in haematocrit, increases in parasitized erythrocytes, pulmonary oedema, hypothermia, formation of prostaglandin-like substances in the central nervous system, increases and decreases in plasma bradykininogen levels and leucocytosis, as evidenced by neutrophilia, lymphocytosis and monocytosis. we found no changes in total plasma prote ... | 1983 | 6362586 |
| anti-lymphocyte autoantibodies in lethal mouse malaria and their absence in an irradiation-induced non-lethal variant. | | 1984 | 6362936 |
| alkali-extraction of membranes from mouse erythrocytes infected with plasmodium berghei. | alkali-extracted membrane material from hypotonically lysed plasmodium berghei-infected mouse erythrocytes has been analyzed by dodecyl sulphate polyacrylamide gel electrophoresis and fractionated by discontinuous sucrose gradient centrifugation. components characteristic for the protein pattern associated with p. berghei infection have been recovered in the alkali-extracted material. parasite components, free of host cell membrane contamination, have been obtained as a high-density fraction fro ... | 1983 | 6362976 |
| plasmodium berghei: eosinophilic depression of infection in mice. | the effects of eosinophilia on the course of plasmodium berghei infection in mice were studied. eosinophilia was induced by intravenous injection of ascaris suum body fluid into the mice. results indicated that eosinophils may play a role in the suppression of murine malaria. a significant reduction in parasitemias and increased survival time in eosinophilic mice occurred compared to mice not treated with a. suum body fluid. reduction of parasitemia was effectively achieved when the mice were ch ... | 1984 | 6363116 |
| synthesis and antimicrobial activity of clindamycin analogues: pirlimycin, 1,2 a potent antibacterial agent. | the preparation of a series of analogues of clindamycin is described in which the naturally occurring five-membered cyclic amino acid amide portion of the molecule is replaced by a four-, six-, or seven-membered cyclic amino acid amide. the most interesting compound is pirlimycin (7e, u-57,930e), in which the (2s-trans)-4-n-propylhygramide portion of clindamycin is replaced by (2s-cis)-4-ethylpipecolamide. this structural modification results in significantly favorable changes in toxicity, metab ... | 1984 | 6363698 |
| circadian temperature rhythm and circadian-circaseptan (about 7-day) aspects of murine death from malaria. | about-7-day (circaseptan) and circadian rhythms were sought and found in host-parasite relations of mice infected with plasmodium berghei. five inbred male dba mice, about 18 weeks of age, were implanted with transsensors for temperature telemetry. core temperature, monitored every 10 min for 3 days before the intravenous or intraperitoneal inoculation of 10(5) infected erythrocytes and thereafter until death, was analyzed by cosinor. a statistically highly significant circadian rhythm exhibited ... | 1984 | 6364151 |
| topographical distribution of the cerebral lesions in mice infected with plasmodium berghei. | in the mouse p. berghei malaria model systematic studies were carried out on the relationship between the type and the topographical distribution of the brain lesion in cerebral malaria. as previously stated for pernicious p. falciparum malaria in man, petechial haemorrhage was not the sole morphologic lesion. in addition to severe brain oedema, microthrombosis, sludging of mononuclear cells, arteriolar spasms, scattered disturbances of the microcirculation, and the occasional proliferation of g ... | 1983 | 6364514 |
| interaction of antimalarial drugs with hemin. | hemin (ferriprotoporphyrin ix) is shown to form complexes with the chloroquine class of antimalarial drugs. the soret band of hemin becomes optically active upon the addition of chiral drugs. results on the hemin-induced quenching of the fluorescence of chloroquine are consistent with the formation of a 2:1 hemin:drug complex with a formation constant of 1.4 x 10(7) at 298 k. also a direct comparison of the drug-treated and drug-free parasites themselves, by the noninvasive photoacoustic spectro ... | 1983 | 6365196 |
| cell-mediated immunity in rats injected with an antimalaria t-cell line. | the aim was to develop a pure t-cell line which would enable the study of some aspects of the cellular immunity of malaria-injected rodents. for this purpose a long-term proliferative antimalaria t-cell line (amtl) was established. the line was developed from splenocytes of rats recovered from a plasmodium berghei infection. after adoptive transfer of the amtl, some protection was demonstrated either by a lower mortality rate after challenge with the parasite or by decreased parasitemia in the t ... | 1984 | 6365332 |
| pulmonary edema in mice infected with plasmodium berghei. involvement of catecholamines. | mice inoculated with plasmodium berghei developed a drastic and significant pulmonary edema. treatment of animals with phenoxybenzamine rendered mice hyporeactive to this physiopathological alteration. | 1984 | 6365585 |
| murine malaria: immune complexes inhibit fc receptor-mediated phagocytosis. | immune complexes have been partially purified from the serum of plasmodium berghei-infected mice by ultracentrifugation on 10 to 40% linear sucrose gradients, by precipitation with polyethylene glycol, and by gel filtration through sephacryl s-300. the complexes contain gamma 1, gamma 2a, gamma 2b, and gamma 3 subclasses of mouse immunoglobulin g in differing amounts, as well as malarial antigen. complexes isolated by all three methods inhibit fc receptor-mediated phagocytosis by normal mouse pe ... | 1984 | 6368390 |
| differences in plasmodium berghei development in nude and normal mice. | | 1984 | 6368438 |
| effect of 60co-irradiation on the development and immunogenicity of plasmodium berghei sporozoites in anopheles stephensi mosquitoes. | protection conferred to mice by plasmodium berghei sporozoites increased significantly when the time interval between 60co-irradiation of the infected mosquitoes and harvest of sporozoites increased. one thousand sporozoites conferred no protection against challenge if harvested on the day of irradiation, but protected 60% of recipient mice when harvested 28 days postirradiation. when the time between feeding of mosquitoes and irradiation was varied, sporozoites from mosquitoes irradiated 3 days ... | 1983 | 6368786 |
| identification of malaria-infected mosquitoes by a two-site enzyme-linked immunosorbent assay. | a micro enzyme-linked immunosorbent assay (elisa) for identifying malaria sporozoites in mosquitoes is described. using an extract of dried infected mosquitoes as antigen, a two-site elisa was sensitive enough to detect one infected mosquito in a pool of 20. the species specificity, sensitivity and ease of performance of this assay, as well as the stability of the reagent, should make it a useful epidemiological tool. | 1984 | 6370003 |
| malarial immunity in pregnant mice, in relation to total and unbound plasma corticosterone. | a pregnancy dependent loss of malarial immunity is accompanied by an (excessive) increase of total as well as free plasma corticosterone. this loss of immunity was largely prevented by adrenalectomy. moreover, malarial immunity was more sensitive to dexamethasone immunosuppression during pregnancy. primary infections are more virulent during pregnancy and like in recrudescent mice, cause excessive total and free plasma corticosterone levels. corticosterone may be considered an immuno-regulatory ... | 1983 | 6370474 |
| differential susceptibility of rodent malaria parasites to nonspecific immunity. | nonspecific immunity to rodent malaria parasites can be produced by immunising mice with heterologous parasites or pretreating them with bcg, corynebacterium parvum or brucella abortus. nonspecific immunity is easily produced against plasmodium vinckei but not p. berghei. this is due to the difference between the cells occupied by the parasites, young red blood cells in the case of p. berghei and mature red blood cells in the case of p. vinckei. | 1983 | 6370475 |
| [effects of anti-infectious immunomodulators in leprosy and malaria in the mouse]. | immunomodulators, whether natural (polysaccharides) or industrial (non-hemolytic detergents) proved active by themselves, in preventive or curative schemes of experimental leprosy and malaria. however, their activity was most often increased, through joint administration with chemotherapeutic agents. | 1983 | 6370479 |
| hepatic superoxide dismutase activity in the mouse infected with plasmodium berghei. | the effects of malarial infection, induced by infecting the mouse with plasmodium berghei (30-40% parasitemia), on hepatic superoxide dismutase (sods) were studied. total sod and cu-zn sod activities were found to be significantly reduced. mn sod activity, however, was not found to be altered. | 1984 | 6370802 |
| malaria parasites do not contain or synthesize sialic acids. | the capacity of plasmodia to synthesize sialic acids was investigated by adding radioactive acetate to short-term in vitro cultures of the intraerythrocytic asexual forms of three malaria parasites (the human malaria plasmodium falciparum in aotus trivirgatus erythrocytes; the simian malaria p. knowlesi in rhesus monkey erythrocytes; the rodent malaria p. berghei in mouse erythrocytes) and to cultures of extracellular zygotes of the avian malaria p. gallinaceum. radioactive acetate was added to ... | 1984 | 6370820 |
| enhancement of phagocytosis of parasitized and nonparasitized red cells in acute plasmodium berghei malaria. | the phagocytosis by mouse peritoneal macrophages of parasitized red cells (prcs) and nonparasitized red cells (nonprcs) separated from plasmodium berghei infected blood was studied in vitro. peritoneal macrophages from acutely infected mice and normal mice were cultured on coverslips. prcs and nonprcs were fractionated by ficoll density gradient centrifugation from plasmodium berghei infected blood. prcs were fed in triplicate cultures to normal macrophages in normal serum, normal macrophages in ... | 1984 | 6371136 |
| anti-intermediate filament antibody in mice infected with plasmodium berghei. | fifteen mice (cba) were infected with lethal plasmodium berghei and the development of anti-intermediate filament antibody (anti-if) studied. sera from all the infected animals reacted with the cytoplasmic network of intermediate filaments (if) in the human epidermal laryngeal carcinoma (hep2) cell line, as demonstrated by indirect immunofluorescence. sera from 5 control animals injected with 10(4) unparasitized red cells showed no anti-if reactivity. anti-if of igm class was first detectable at ... | 1984 | 6371238 |
| [sensitivity of the erythrocytes of mice infected with plasmodium berghei to saponin and a hypotonic solution]. | | 1984 | 6371469 |
| comparison of in vitro and in vivo antimalarial activities of 9-phenanthrenecarbinols. | analysis of the antimalarial activity of a selected series of 17 9-phenanthrenecarbinols against cultured strains of plasmodium falciparum and against p. berghei in mice following oral administration indicated that the rankings of activities within the series were influenced by substituents on the 9-carbinol and the route of administration. compounds with alkylamino-alkyl groups were ranked as most active by an in vitro screening system which assayed activity against chloroquine-sensitive and ch ... | 1984 | 6372713 |
| lysosomal enzymes in the spleen of albino rats, mice and mastomys natalensis during plasmodium berghei infection. | activities of certain acid hydrolases of the spleen were followed in three different rodents during the course of plasmodium berghei infection. in albino rats where sterile immunity against the infection develops, the specific activities of a few acid hydrolases first declined then increased from day 7 and showed a several-fold increase over control values when there were no detectable parasites in peripheral circulation. in contrast, in mastomys natalensis and albino mice which succumb to infec ... | 1983 | 6372771 |
| metabolism of erythrocyte infected with malaria parasite and the action of antimalarial drugs. | | 1983 | 6372794 |
| isolation of a single messenger rna and of the corresponding gene in plasmodium berghei. | a genomic library of plasmodium berghei dna was constructed using lambda 47.i as a vector. it represents 90% of plasmodium genome. genes expressed during the intraerythrocytic stage of p. berghei were isolated among the recombinant clones of the library using labelled cdna complementary to the polya + plasmodium mrna extracted during this stage. the purified coding strand of an expressed clone was utilized to catch the corresponding mrna(s). the hybridized mrna fraction was eluted and in vitro t ... | 1984 | 6373026 |
| role of macrophages in malaria: o2 metabolite production and phagocytosis by splenic macrophages during lethal plasmodium berghei and self-limiting plasmodium yoelii infection in mice. | the role of splenic macrophages in resistance to lethal plasmodium berghei or self-limiting plasmodium yoelii was studied by testing their rate of phagocytosis and their production of o2 metabolites (h2o2 and o2-) upon nonspecific stimulation with zymosan. it was found that, compared with p. berghei, infection of mice with p. yoelii resulted in an earlier appearance and in higher numbers of adherent cells in the spleen. furthermore, the capacity of macrophages to generate o2 metabolites was sign ... | 1984 | 6373617 |
| effect of salinomycin-na on malaria parasites (plasmodium falciparum and p. berghei). | in vitro exposure of plasmodium falciparum and p. berghei to salinomycin-na showed that 10 min incubation in rpmi 1640 medium containing 100 micrograms/ml of the polyether antibiotic led to complete destruction of most parasites; in media containing 10 or 1 microgram/ml salinomycin-na some young developmental stages seemed to survive, apparently due to the protection of the mostly intact host cell. in vitro treatment of rats infected with p. berghei revealed that a single subcutaneous (oral) dos ... | 1984 | 6375210 |
| action of a new floxacrine derivative (s 82 5455) on asexual stages of plasmodium berghei: a light and electron microscopical study. | the floxacrine derivative s 82 5455, 7-chloro-1-( 4n - methylpiperazino -1n-imino)-10-hydroxy-1, 2, 3, 4-tetrahydro-3-(4-trifluoromethylphenyl)-9(10 h)- acridone , shows a high activity against blood induced infection of a drug-sensitive line of plasmodium berghei in mice and rats. the dosis curativa minima/ dosis tolerata maxima values against the drug-sensitive p. berghei strain ascertained in the '28-day test' in mice, were 1.56 (x 5)/400 (x 1) mg/kg after the oral route 3.12(x 5)/400(x 1) mg ... | 1984 | 6375212 |
| host defenses in murine malaria: humoral immunity to plasmodium berghei in mice. | humoral immunity to plasmodium berghei infection of f1 hybrid b6d2 (c57b1/6 x dba/2) mice was investigated using an immune serum prepared from mice which survived a lethal challenge of erythrocytic stage p. berghei because of previous vaccination with formalin-killed p. berghei. immune serum, but not normal serum, if injected intraperitoneally or intravenously soon after infection, during rapidly increasing parasitemia, transiently inhibited the progress of infection in a pattern which was direc ... | 1984 | 6375406 |
| the malarial pigment in rat infected erythrocytes and its interaction with chloroquine. a mössbauer effect study. | mössbauer studies of rat erythrocytes infected by plasmodium berghei malaria parasites, using 57fe-enriched rat red blood cells, were carried out in order to determine the physical parameters which characterize the malarial pigment iron and to test the effect of the widely used antimalaria drug, chloroquine, on these parameters. the iron in the malarial pigment which is derived from hemoglobin digestion by the intracellular parasite was found to be trivalent, high spin, with mössbauer parameters ... | 1984 | 6376502 |
| susceptibility of anopheles punctipennis and other florida mosquitoes to plasmodium berghei. | | 1984 | 6376750 |
| riboflavin deficiency inhibits multiplication of malarial parasites. | | 1984 | 6377134 |
| lipoprotein lipase suppression in 3t3-l1 cells by a haematoprotozoan-induced mediator from peritoneal exudate cells. | lysates of the haematoprotozoa trypanosoma brucei or plasmodium berghei stimulated murine peritoneal exudate cells to release a mediator, which suppressed lipoprotein lipase activity in differentiating 3t3-l1 preadipocytes. the parasite-induced mediator suppressed the activity of cell surface lipoprotein lipase up to 39% in a dose dependent manner. by impairing the activity of cell surface lipoprotein lipase, this mediator acts to inhibit the uptake of fatty acid, and ultimately the accumulation ... | 1984 | 6377200 |
| lipid antigens derived from erythrocytes infected with plasmodium berghei. | lipids were extracted from red blood cells infected with plasmodium berghei, from the membranes of infected red cells and from free parasites. a radioimmunoassay was used to detect antibodies to these lipids in sera from convalescent and immune rats. most of the antigenic activity could be attributed to the parasite although some activity was found in lipids isolated from the membranes of infected red blood cells. absorption studies showed that the binding was specific for malarial lipid antigen ... | 1984 | 6377726 |
| interrelationship of age of host, immunity & plasmodium berghei infection. | | 1984 | 6378778 |
| antigenic analysis of soluble extract of plasmodium berghei using sodium dodecyl sulphate polyacrylamide gel electrophoresis. | | 1984 | 6378779 |
| identification of 5 s and 5.8 s ribosomal rna molecules and their genes in plasmodium berghei. | the ribosomes of plasmodium berghei contain two small rna molecules approximately 150 and 120 bases long. these correspond in size to 5.8 and 5 s rna molecules found in the cytoplasmic ribosomes of eukaryotic cells. segments homologous to the 5.8 s rna are present in each of the four ribosomal rna gene units. restriction fragments which span the region between the coding areas of the two major ribosomal rna molecules hybridize to 5.8 s rna, indicating that the gene is located between the small a ... | 1984 | 6379449 |
| two major sequence classes of ribosomal rna genes in plasmodium berghei. | primary sequence differences have been found between two different ribosomal dna (rdna) units of the rodent malaria parasite, plasmodium berghei, within the coding areas of both the small and large ribosomal rnas (rrna). the coding regions of rdna unit a are protected from nuclease s1 digestion by rrna isolated from asexual blood stage parasites. under the same conditions of analysis, the comparable coding regions from unit c are cut into small pieces by nuclease s1, the largest being 1.1 kb. an ... | 1984 | 6379606 |
| [study of anemia and thrombopenia in plasmodium berghei malaria]. | | 1984 | 6379829 |
| establishment of ancylostoma ceylanicum adult infection in plasmodium berghei-infected albino mice(mus musculus) | | 1984 | 6380028 |
| a simple method for isolating plasmodium berghei-infected erythrocytes from experimentally infected animals. | | 1984 | 6380033 |
| [action of chloroquine on glutathione metabolism in erythrocytes parasitized by plasmodium berghei]. | chloroquine acts on erythrocytes parasitized by a p. berghei sensitive strain, inducing a dramatic decrease of the intra erythrocytic reduced glutathione. this reduction follows a decrease of the glutathione reductase activity. contrarily when erythrocytes are parasitized by a p. berghei resistant strain neither the intra erythrocytic reduced glutathione nor the glutathione reductase activity are modified by the action of chloroquine. glutathione metabolism could be the main target of action of ... | 1984 | 6380377 |
| plasmodium berghei infection in mice: effect of low-level ozone exposure. | | 1984 | 6380627 |
| [growth and decline of parasitemia in trophozoite-induced plasmodium berghei and plasmodium yoelii infection in mice]. | | 1983 | 6380802 |
| relation between haemoglobin degradation and maturity of the red blood cell infected by p. berghei. | the action on haemoglobin of p. berghei growing in mature red cells, p. berghei growing in reticulocytes and p. berghei r.c. (which grows almost exclusively in reticulocytes) was compared. p. berghei growing in reticulocytes had a much higher level of proteolytic activity on haemoglobin than that of p. berghei growing in mature red cells. the amount of residual hematin was considerably reduced. in p. berghei r.c. and p. berghei growing in reticulocytes, the pigment seems to be exocyted as it is ... | 1984 | 6380913 |
| plasmodium berghei: the effects of suppressor factor on vaccination. | | 1984 | 6381347 |
| long-term in vitro cultures of plasmodium berghei and preliminary observations on gametocytogenesis. | | 1984 | 6381348 |
| glutathione metabolism in malaria infected erythrocytes. | | 1984 | 6382309 |
| ferriprotoporphyrin ix: a mediator of the antimalarial action of oxidants and 4-aminoquinoline drugs. | ferriprotoporphyrin ix (fp) is released from hemoglobin by oxidative denaturation or by proteolytic degradation. fp added exogenously to cells or released intracellularly is a lytic toxin. chloroquine enhances the accumulation of exogenous fp in cellular membranes and potentiates its lytic effect. menadione is an example of an oxidant drug that denatures hemoglobin, releases fp intracellularly, and thereby lyses cells. chloroquine increases the accumulation of fp in the membranes of menadione-tr ... | 1984 | 6382310 |
| host superoxide dismutase incorporation by intraerythrocytic plasmodia. | | 1984 | 6382311 |
| chloroquine resistance and host cell hemoglobin catabolism in plasmodium berghei. | | 1984 | 6382314 |
| evolutionary relatedness of plasmodium species as determined by the structure of dna. | malaria parasites can be grouped evolutionarily by analysis of dna composition and genome arrangement. those that vary widely with regard to host range, morphology, and biological characteristics fit into only a small number of distinctive groups. the dna of the human parasite plasmodium falciparum fits into a group that includes rodent and avian malarias and is unlike the dna of other primate malaria parasites. the dna of plasmodium vivax, which is also a human parasite, fits into a distinctly ... | 1984 | 6382604 |
| h-2 dependent differential course of experimental plasmodium berghei infections. | | 1983 | 6382900 |
| [mathematical model of the dynamics of parasitemia in mice infected with p. berghei]. | | 1984 | 6384757 |
| evidence for major differences in ribosomal subunit proteins from plasmodium berghei and rat liver. | purified polysomes were isolated in high yield from the erythrocytic stages of the rodent malaria parasite, plasmodium berghei, and from rat liver. proteins extracted from the ribosomal subunits derived from these polysomes were fractionated and their number and molecular weights were estimated by two-dimensional polyacrylamide gel electrophoresis. plasmodial small ribosomal subunits contained 30 proteins ranging in apparent molecular size from 11.7 to 40.7 kda, while large subunits contained 35 ... | 1984 | 6384776 |
| increased phagocytosis of non-parasitized red cells in plasmodium berghei malaria. | | 1984 | 6385888 |
| early lymphocyte trapping in malaria infections: a particulate antigen mediated phenomenon. | during the course of rodent malaria a marked decrease in the numbers of circulating lymphocytes within the peripheral blood occurred 2-4 days post-infection. monocytes and polymorphs did not show the same degree of decline. for both avirulent plasmodium yoelii and lethal plasmodium berghei infections lymphocyte numbers returned to control levels by day 6-8 post-infection. while these levels were maintained until clearance of p. yoelii infection, a sustained and abnormal increase occurred during ... | 1984 | 6387079 |
| blood changes and enhanced thromboxane and 6-keto prostaglandin f1 alpha production in experimental acute plasmodium bergei infection in hamsters. | golden hamsters inoculated intraperitoneally with plasmodium bergei infected mouse blood regularly developed p. bergei parasitaemia. this was associated with progressive thrombocytopenia and leucocytosis as the degree of parasitaemia increased with time. when infected whole blood was stimulated with collagen, significantly enhanced thromboxane b2 (txb2) production per platelet was seen. 6-keto prostaglandin (pg) f1 alpha formation in the same system increased from the sixth infection day onwards ... | 1984 | 6388013 |
| fine structure of plasmodium berghei exoerythrocytic forms in cultured primary rat hepatocytes. | sporozoites of the rodent malaria parasite plasmodium berghei have been grown in primary cultures of hepatocytes from brown norway rats. the ultrastructure of in vitro grown exoerythrocytic forms was compared with that of parasites in vivo. peculiar vesicles, previously not described in vivo, were identified and their possible origin is discussed. otherwise, the fine structure of the hepatocytic stages grown in vitro was shown to be grossly similar to those in vivo. therefore, electron microscop ... | 1984 | 6388852 |
| characterization of macrophage dysfunction in rodent malaria. | immunosuppression in malaria has been attributed, in part, to alterations in macrophage function. the present study was undertaken in an attempt to characterize the dysfunction and to determine if it is regional or if it occurs in different populations of macrophages. the resting o2 consumption of either hepatic, splenic, or peritoneal macrophages or polymorphonuclear neutrophils (pmns) was unaltered by the malaria infection. however, the respiratory burst was significantly enhanced in the three ... | 1984 | 6389742 |
| antigen-specific and concanavalin a-induced lymphocyte blastogenesis responses during the course of plasmodium berghei infection in rats. | antigen specific and concanavalin a (con a)-induced lymphocyte blastogenesis responses were monitored in the spleens of rats infected with plasmodium berghei. con a responses were depressed only at the time of peak parasitaemia. the antigen specific blastogenesis response was either not in evidence, or at a low level during the periods of patent or subpatent infection (up to 8 weeks after infection). higher levels of blastogenesis were seen from 8 to 12 weeks after infection, which correlates wi ... | 1984 | 6390300 |
| detection and characterization of a selective endopeptidase from plasmodium berghei by using fluorogenic peptidyl substrates. | by using fluorogenic peptidyl-3-amino-9-ethyl-carbazole a highly selective endopeptidase for the val-leu-gly-arg sequence was demonstrated in endoerythrocytic stages of plasmodium berghei. val-leu-gly-arg-endopeptidase showed a maximum activity in ph range 7.0-8.0; it was completely inhibited by 1 mm leupeptin and 1 mm antipain. a complete inhibition was also obtained by 15 mm chloroquine. this trypsin-like activity was negligible in uninfected red blood cells. the high sensitive fluorogenic pro ... | 1984 | 6391479 |
| characterization of thymidylate synthetase and dihydrofolate reductase from plasmodium berghei. | | 1984 | 6392123 |
| 13c nmr studies of glycolysis in intra- and extra-erythrocytic babesia microti. | metabolism in the erythrocytes of normal mice and mice infected with babesia microti has been monitored non-invasively by high resolution 13c nmr spectroscopy. the conversion of [u-13c]glucose to lactate in both normal and infected cells together with the effect of the trypanocidal drug, 4,4'-diamidinodiazoaminobenzene diaceturate, on glycolytic rates were monitored. these studies show that erythrocytes utilize [u-13c]glucose at a rate of 3 x 10(-12) mumol cell-1 min-1 at 35 degrees c while para ... | 1984 | 6392883 |
| [preferential action of chloroquine on plasmodium within mature erythrocytes]. | in vitro studies on the effects of chloroquine on plasmodium berghei in relation to the age of the host cell made it possible to demonstrate the preferential effect of the drug on parasites growing in mature red blood cells. the ed-50 for parasites in mature red blood cells is lower (1,64 +/- 0,2 mg/kg/day) than in reticulocytes (2,45 +/- 0,2 mg/kg/day). the result of clumping test for chloroquino-sensitive p. berghei growing in young red blood cells, is almost similar as in a chloroquino-resist ... | 1984 | 6393008 |
| intranuclear localization of plasmodium berghei sporozoites. | | 1984 | 6394151 |
| [preliminary observation on the pre-erythrocytic schizont of plasmodium berghei anka strain]. | | 1984 | 6394173 |
| effects of immune complexes on immunity to plasmodium berghei infection. | the elisa test titers and ripa patterns of sera collected from vaccinated and non-vaccinated rats during p. berghei infection were similar. the sera collected just after clearance of parasitemia from the vaccinated rats, but not that from the non-vaccinated rats protected mice in passive protection tests. after precipitation to remove immune complexes, the sera from the non-vaccinated rats also protected mice. administration of acute phase serum early in the course of infection aggravated parasi ... | 1984 | 6395456 |
| the chemotherapy of rodent malaria xxxv. further studies on the retardation of drug resistance by the use of a triple combination of mefloquine, pyrimethamine and sulfadoxine in mice infected with p. berghei and 'p. berghei ns'. | in the face of an increasing prevalence of plasmodium falciparum resistant to chloroquine and to pyrimethamine-sulphonamide or -sulphone mixtures, the need for a new, effective blood schizontocide for treatment of acute malaria is urgent. the only such compound that is almost ready for release is mefloquine (m) but there is already a danger that parasites may become resistant to this compound if it is used extensively alone. earlier studies using a rodent model indicated that mefloquine could be ... | 1984 | 6395814 |
| plasmodium berghei: characterization of antigens and their role in inducing immunity to infection. | in vitro, plasmodium berghei infected erythrocytes incorporated 35s-methionine into 31 polypeptides with molecular weights from 21 kd to 300 kd. hemoglobin and additional smaller molecular weight polypeptides were labelled with 35s-methionine by a population of uninfected, reticulocyte-rich rat erythrocytes. 3h-glucosamine was incorporated into at least 3 components by plasmodium berghei infected erythrocytes. uninfected, reticulocyte-rich rat erythrocytes did not incorporate 3h-glucosamine. rab ... | 1984 | 6396392 |
| 2-acetylpyridine thiosemicarbazones xi: 2-(alpha-hydroxyacetyl)pyridine thiosemicarbazones as antimalarial and antibacterial agents. | a series of 2-(alpha-hydroxyacetyl)pyridine thiosemicarbazones was synthesized as potential antimalarial and antibacterial agents. their synthesis was achieved by the condensation of n4-mono- or n4,n4-disubstituted thiosemicarbazides with 2-(alpha-hydroxyacetyl)pyridine. the latter was prepared by selective bromine oxidation of (2-pyridinyl)-1,2-ethanediol. the new compounds show potent inhibitory activity against penicillin-sensitive as well as penicillin-resistant neisseria gonorrhoeae (mic, 0 ... | 1984 | 6396400 |
| impairment of hepatic cytochrome p-450-dependent monooxygenases by the malaria parasite plasmodium berghei. | the effect of plasmodium berghei infection on hepatic monooxygenase activities and cytochrome p-450 contents was investigated in mice. nih/nmri or a/j mice infected with active p. berghei showed 30-40% decreases in hepatic cytochrome p-450 contents and the ability to metabolize the test substrates, ethylmorphine and benzo(a)pyrene. these decreases were observed during the erythrocytic stage of the infection, but not during the initial exoerythrocytic stage, or after heat-inactivated sporozoites ... | 1984 | 6396516 |
| 2-acetylpyridine thiosemicarbazones. 10. 2-propionyl-, 2-butyryl-, and 2-(2-methylpropionyl)pyridine thiosemicarbazones as potential antimalarial agents. | in view of the antimalarial properties observed for many 2-acetylpyridine thiosemicarbazones, a series of n4,n4-disubstituted thiosemicarbazones derived from 2-propionyl-, 2-butyryl-, and 2-(2-methylpropionyl)pyridine was prepared for evaluation against the malaria parasite, plasmodium berghei, in the mouse. the thiosemicarbazones were made by the reaction of methyl hydrazinecarbodithioate with a 2-acylpyridine to give the intermediate methyl 3-[1-2-pyridinyl)alkylidene]hydrazinecarbodithioates. ... | 1984 | 6397197 |
| use of 13c nuclear magnetic resonance spectroscopy for the evaluation of hepatic drug-metabolizing enzyme activity in perfused mouse livers. | experiments were performed to determine whether the activity of hepatic drug-metabolizing enzymes could be estimated in intact livers by 13c nmr spectroscopy. 13c-labelled aminopyrine was administered to isolated perfused mouse livers and the rate of decline in the nmr signal arising from the drug was used to estimate the half-life of aminopyrine. the technique was sufficiently sensitive to discriminate between the enzyme activities in normal livers and in livers whose enzyme activity was enhanc ... | 1984 | 6397498 |
| the chemotherapy of rodent malaria, xxxviii. studies on the activity of three new antimalarials (wr 194,965, wr 228,258 and wr 225,448) against rodent and human malaria parasites (plasmodium berghei and p. falciparum). | in addition to their blood schizontocidal action on plasmodium berghei in vivo, two mannich bases wr 194,965 and 228,258 are also active against chloroquine-sensitive and chloroquine-resistant lines of p. falciparum in vitro. the response of the lines to each drug differs but shows no correlation in either case with response to chloroquine. the 8-aminoquinoline wr 225,448 is also active against p. falciparum in vitro but at much higher concentrations than the mannich bases. application of the 'c ... | 1984 | 6398032 |
| peroxidative changes in erythrocytic enzymes in plasmodium berghei induced malaria in mice. | | 1984 | 6398277 |
| increased severity of malaria infection in rats fed supplementary amino acids. | infection with plasmodium berghei malaria is severely inhibited in rats fed on a low protein diet. a range of amino acid supplements was added to a 4.2% casein diet to determine whether the relationship between level of infection and protein content could be attributed to the dietary amounts of the essential amino acids. significant increases in levels of infection were achieved by supplementation with specific combinations of amino acids. threonine was most effective in increasing the degree of ... | 1984 | 6398537 |
| experimental malarial infection. ii--plasmodium berghei infection in normal and b-cell deficient mice. | | 1984 | 6399259 |
| experimental malarial infection. iii--protective role of antibodies in plasmodium berghei infection in mice. | | 1984 | 6399260 |
| [rodent malaria model of plasmodium berghei anka strain for antimalarial screening: its establishment and use]. | | 1984 | 6399808 |
| inheritance of post-vaccinal resistance against plasmodium berghei infection in mice. | the protective effect of specific vaccination against plasmodium berghei (p. berghei) was compared in terms of survival percentage in dba/2, c3h, outbred albino mice and in two lines of mice produced by selective breeding for either high or low antibody responsiveness to sheep erythrocyte (h and l lines respectively). the efficacy of induced protection varies according to genetic constitution. it is very strong in h line and albino mice, intermediate in dba/2 and very weak in l line and c3h. the ... | 1983 | 6405360 |
| reduction of ca2+ uptake induced by ionophore a23187 of red cells from malaria (plasmodium berghei)-infected mice. | the ca2+ ionophore a23187 has much less capacity to induce ca2+ uptake of red cells from p. berghei-infected mice than of cells from normal mice. the reduction in ca2+ uptake occurs in both uninfected and infected cells at all stages in the infected blood, as shown from experiments with cells separated on colloidal silica density gradient. measurement of the ionophore concentration in the medium reveals that the ionophore is partitioned into red cells from infected blood to a greater extent than ... | 1983 | 6406077 |
| [use of mefloquine as an antimalarial]. | | 1983 | 6411373 |
| plasmodium berghei: inhibition of the sporogonous cycle by alpha-difluoromethylornithine. | alpha-difluoromethylornithine (dfmo), an enzyme inhibitor of ornithine decarboxylase, inhibits the sporogonous cycle of the malaria parasite plasmodium berghei in the mosquito vector anopheles stephensi. dfmo was administered to the mosquitoes dissolved either in the sugar solution at their disposal in the cages or through blood meals taken from treated mice. the mice subsequently bitten by mosquitoes treated with dfmo by both routes of administration did not contract malaria. | 1983 | 6413237 |
| mechanism of enhanced fusion capacity of mouse red cells infected with plasmodium berghei. | plasmodium berghei-infected mouse red cells have enhanced fusion capacity as triggered by addition of poly(ethylene glycol) in the presence of ca2+. the uptake of ca2+ in p. berghei-infected cells is greater than in normal cells, and the difference in ca2+ uptake was found to be enhanced in the presence of poly(ethylene glycol). fusion of normal and p. berghei-infected red cells by poly(ethylene glycol) was significantly inhibited by n-tosyl-l-lysylchloromethyl ketone and phenylmethylsulphonyl f ... | 1983 | 6415072 |
| micro-counterimmunoelectrophoresis: a rapid screening technique for trypanosomiasis. | a rapid and economical micro-counterimmunoelectrophoresis (mcie) test, for antibody to trypanosome antigens, has been developed which may prove useful in trypanosomiasis surveillance. mcie was more sensitive and more rapid than immunodiffusion (id). serum samples from trypanosome-infected rabbits and cattle (nigerian and gambian), from kuwaiti dogs and camels, from ivory coast sleeping sickness patients, from brazilian patients with chagas's disease, plus mouse-anti-malaria sera have been tested ... | 1983 | 6415874 |
| [biochemical aspects of the interaction of the malarial parasite, plasmodium berghei, with erythrocytes of the host]. | mice erythrocytes, infected with plasmodium berghei, were studied as a model of integrated cell system "parasite-host erythrocytes". the rate of blood oxygenation, content of methhemoglobin and methhemoglobin reductase activity maintained at the initial level up to the lethal outcome of the infectious process. a steady-state kinetics of glycolysis was studied in the impaired erythrocytes by means of automatic potentiometric titration of lactic acid during incubation of the cells. distinct 15-20- ... | 1983 | 6417909 |
| interactions of protein calorie malnutrition, malaria infection and immune responses. | the course of parasitaemia and certain immune responses in protein or calorie-deficient albino rats infected with plasmodium berghei (nicd) were studied. wide variations observed in the course of parasitaemia in protein-deficient animals were (a) prolonged prepatent period, short patent period with low parasitaemia (50% of animals), (b) short prepatent period and an inability to resolve the infection (14% of animals) and (c) no patent parasitaemia at any stage of observation (23.5% of animals). ... | 1983 | 6419716 |
| plasmodium berghei sporozoites are mitogenic for murine t cells, induce interferon, and activate natural killer cells. | enhanced natural killer (nk) activity was detected in the spleens of mice as early as 24 hr after single i.v. inoculation with gamma-irradiated plasmodium berghei sporozoites. the activity peaked at 48 hr post-injection, and declined below baseline level by day 8. reinoculation of mice with irradiated sporozoites produced an increased nk activity significantly smaller than the original activity. spleen cells sensitized in vivo as well as nonsensitized spleen cells stimulated in vitro with sporoz ... | 1984 | 6429240 |
| the antimalarial activity of n-benzyloxydihydrotriazines. ii. the development of resistance to clociguanil (brl 50216) and cycloguanil by p. berghei. | | 1980 | 6450572 |
| glycolytic metabolism in malaria infected red cells. | | 1981 | 6457304 |
| differential involvement of non-specific suppressor t cells in two lethal murine malaria infections. | the suppression of the contact sensitivity of oxazolone in murine malaria is shown to be mediated by non-specific t suppressor cells, but to a different extent in infection caused by two different species of parasite. depletion of t suppressor cells in vivo and/or anti-thy 1.2 treatment in vitro indicated that in mice infected with p. berghei the suppressor effect was largely mediated by t cells. by contrast, in mice infected with a lethal strain of p. yoelii it was only partly due to t cells; b ... | 1981 | 6459199 |
| plasmodium berghei: acid-insensitive phosphofructokinase in infected mouse erythrocytes. | | 1982 | 6459948 |