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variants of n-tropic leukemia virus derived from balb/c mice.clonal lines derived from cultures of nih/3t3 cells infected with n-tropic leukemia virus from balb/c mice differ in the amount and type of n-tropic virus they produce. three biologically distinguishable n-tropic viruses were found: the large xc plaque-forming virus of hartley et al. (1969) (lp-n), a small xc plaque-forming virus (sp-n), and a non-plaque-forming virus (np-n). sp-n and np-n are less infectious than lp-n. upon prolonged passage in nih/3t3 cells np-n gives rise to highly infectious ...197551938
immunological relationships of bacterial l-asparaginases.rabbit antisera against l-asparaginase preparations from escherichia coli, erwinia carotovora, citrobacter sp. and chromobacterium violaceum showed on immunoelectrophoresis that only the enzymes from e. coli and citrobacter are immunologically related. purified preparations had to be used to determine the immunological cross-reactions. immunoelectrophoresis at different ph values yielded the zero mobility points of the enzymes. the activity of the er. carotovora preparation was enhanced up to fo ...197654404
chikungunya virus in vivo autointerference associated with a small plaque variant.virulent and avirulent plaque variants of chikungunya (chik) virus were compared with the prototype and other strains. interference with infective virus growth or mouse pathogenicity only occurred in the presence of small plaque (sp) variants in homologous systems. possible mechanisms for this are suggested.197835947
parital characterization of the rat anti-encephalitogenic protein (rscp).a protein antigenically similar to the anti-encephalitogenic bovine spinal cord protein (bscp) was detected in saline extracts of rat nervous tissues by immunodiffusion analyses using a rabbit anti-bscp serum. rat scp (rscp) appears to be evenly distributed throughout all parts of the rat nervous system and occurs also in the thymus, thyroid, and adrenal glands. although immunodiffusion analyses indicated that rscp shares some antigenic sites with bscp, anti-rscp sera reacted only with rscp, ind ...197655440
regulation of slow potential shifts in nucleus reticularis thalami by the mesencephalic reticular formation and the frontal granular cortex.novel stimuli or electric stimulation of the mesencephalic reticular formation (mrf) produced large positive slow potentials (sps) in rostral nucleus reticularis thalami (rva) that accompanied the negative sps known to occur in frontal cortex. sp durations (20-30 sec) were similar to the periods of unit inhibition that occur in rva following mrf stimulation. trains of 8 c/sec medial thalamic stimuli produced phasic negative sps in rva similar in duration to the intervals of unit excitation that ...197657857
[management in pollinosis and false pollinosis].typical cases of pollen allergen (hayfever, allergic asthma), together with isolated non-respiratory "equivalent" manifestations (urticaria, eye conditions, headache, etc.), are easy to detect on the basis of skin tests and the clinical history. such manifestations may also occur in "false pollen allergy", related in most instances by atmospheric moulds (dematiaceae), sometimes by house dust or dermatophytes (candida albicans, trichophyton sp), by food or by a bacterial infection or allergy. a c ...197836022
purification and properties of amylase produced by a moderately halophilic acinetobacter sp.a moderately halophilic acinetobacter sp., capable of producing dextrinogenic amylase, was isolated from sea-sands. maximum enzyme production was obtained when the bacterium was cultivated aerobically in media containing 1 to 2m nacl or 1m kcl. two kinds of amylase, amylases i and ii were purified from the culture filtrate to an electrophoretically homogenous state by glycogen-complex formation, deae-sephadex a-50 chromatography, and sephadex g-200 gel filtration. both enzymes had maximal activi ...197830530
hydrolysis of phosphatidylethanolamine and phosphatidylglycerol by outer membrane phospholipase a from acinetobacter sp. ho1-n.an outer membrane phospholipase a active against phosphatidylglycerol and phosphatidylethanolamine was characterized from acinetobacter sp. ho1-n.197830758
the action of lithium carbonate on the sleep-waking cycle in the cat.the therapeutic effect of lithium carbonate on manic-depressive psychosis is now universally recognized. in view of this positive action on the cyclic endogenous manifestations, it was interesting to investigate the possible effects of lithium on the sleep-waking cycle in the cat. polygraphic recording from three adults cats was carried out during 24 h periods before, and on different days after, beginning treatment with lithium carbonate. with low doses (30 and 50 mg/kg/day), important morpholo ...197767027
sucrose-negative variants of candida tropicalis.four cultures of a candida sp. that lacked alpha-glucosidase activity were isolated from clinical specimens. physiological, morphological, and serological characterizations of the yeasts and deoxyribonucleic acid reassociation studies supported their classification as a variant of c. tropicalis.197767125
characterization of human papovavirus rfv: comparison with sv40 and bkv.human papovavirus, rfv, isolated from urine of a renal transplant patient was compared with two strains of sv40 and with the prototype human papovavirus, bkv. neutralization tests showed that rfv and bkv are indistinguishable, while large-plaque (lp) and small-plaque(sp) isolates of sv40 gave a low but significant level of cross-reaction with rabbit or human antisera against rfv. dna reassociation saturation tests using 125i-labelled rfv dna show that bkv has 88% homology, and sp-sv40 has 29% ho ...197767183
specific inhibition of viral neuraminidases by an inhibitor, neuraminin, produced by streptomyces sp. 197767704
adenylate cyclase in bloodstream forms of trypanosoma (trypanozoon) brucei sp.1. the adenylate cyclase in trypanosoma brucei is located in the plasma membrane. 2. a partial kinetic analysis of the properties of the enzyme revealed a km for atp of 1.75 mm and a km for mg2+ of 4mm. 3. at low concentrations, mg2+ activated the enzyme directly in addition to its effect of lowering the concentration of inhibitory free atp species. 4. at high concentrations, mg2+ inhibited the enzyme. furthermore, the enzyme was inhibited at any mg2+ concentration if the concentration of atp ex ...197832875
microbial metabolism of amino alcohols. purification and properties of coenzyme b12-dependent ethanolamine ammonia-lyase of escherichia coli.1. the 120-fold purification of ethanolamine ammonia-lyase from escherichia coli extracts, to apparent homogeneity, is described. ethanolamine, dithiothreitol, glycerol and kcl protected the apoenzyme from inactivation. 2. at the optimum ph7.5, k(m) values for ethanolamine and coenzyme b(12) were 44mum and 0.42mum respectively. the k(m) for ethanolamine was markedly affected by ph, transitions occurring at ph7.0 and 8.35. 3. the enzyme was specific for ethanolamine as substrate, none of the 18 a ...197833657
synthesis of cellulase by fusarium sp. in different culture conditions.the mechanism of the synthesis of cellulases by fusarium sp. strain was studied. it was found that a significant role in determination of cellulases is played by the adsorption of these enzymes on cellulose. the aeration level of the culture media had a significant effect on the synthesis as well as on the enzymatic complexity of cellulases.197770972
cell envelope of neisseria gonorrhoeae cs7: peptidoglycan protein complex.treatment of cells grown to exponential phase with 4% sodium dodecyl sulfate for 3 h at 100 degrees c resulted in solubilization of all cellular components except for peptidoglycan. in most strains, cells cultured in liquid gonococcal broth at ph 7.2 yielded a peptidoglycan composed primarily of n-acetylmuramic acid n-acetylglucosamine, alanine, glutamic acid, and diaminopimelic acid in a molar ratio of 1:1:2:1:1. the peptidoglycan in these cells accounted for 1 to 2% (dry weight) of the cells. ...197933903
substance p: characteristics of binding to synaptic vesicles of rat brain.1. the binding of substance p (sp) to synaptic vesicles from rat brain was studied by use of the 125i-tyr8-analogue of sp. 2. the ph dependence of the binding of both peptides to the lipid extractable fraction of synaptic vesicles was shown to be comparable. 3. the binding of 125i-tyr8-sp shows a rate constant of association (k1 = 6.6 x 10(6) m-1 s-1), a rate constant of dissociation (k-1 = 6.4 x 10(-4) s-1) and gives a kd of 1 x 10(-10) m. kd derived from equilibrium studies was 3.2 x 10(-10) m ...197934108
immunodiffusion studies on schistosoma mansoni and its intermediate host stage specific antigens. 3. immunoelectrophoresis cross-reactions between s. mansoni stages and biomphalaria sp. hepato-pancreas antigens.immunoelectrophoresis was applied for cross-wise checking of the different stages in the life cycle of schistosoma mansoni with extracts of hepato-pancreas from its intermediate host snails biomphalaria glabrata and biomphalaria alexandrina. only few fractions were common to non infected snail organ and parasite development stage, while more crossreactions were detected with infected snail hepato - pancreas.197877220
bovine fetus-specific serum proteins. purification and characterization of alpha1-fetoprotein and immunological identification of alpha2- and beta-fetoproteins.bovine alpha1-fetoprotein was isolated from fetal calf serum by successive procedures of concanavalin a-sepharose chromatography, deae-sephadex chromatography, sp-sephadex chromatography and preparative disc polyacrylamide gel electrophoresis. the bovine alpha1-fetoprotein preparation was considered homogeneous by several physicochemical and immunochemical criteria. bovine alpha1-fetoprotein has a molecular weight of 68 000 with an amino acid composiotn similar to that of other mammalian alhpa1- ...197878727
factors affecting the incidence and anti-salmonella activity of the anaerobic caecal flora of the young chick.thirty-two different types of anaerobic bacteria isolated from chickens have been tested for anti-salmonella activity in vitro. under the conditions of the test only bacteroides hypermegas and a bifidobacterium sp. were shown to inhibit the salmonellas and this was attributed to the production of volatile fatty acids (vfa's) coupled with a low ph. when these organisms were tested in newly hatched chicks no inhibition of s. typhimurium occurred. possible explanations for this observation are cons ...197934658
detection of goitrin and its heptafluorobutyryl derivative by gas-liquid chromatography with electron capture, electrolytic conductivity and sulfur detectors.the separation of goitrin by two gas-liquid chromatographic columns, the response of different detectors and the use of the heptafluorobutyric (hfb) anhydride derivative of goitrin to improve sensitivity was investigated. the non-polar 3% ov-1 and the intermediate 4% se-30/6% sp-2401 on 80-100 mesh chromosorb w hp gave comparable results and were considered interchangeable. the sensitivities of the electron capture, sulfur 394 nm emission, chlorine and nitrogen coulson electrolytic conductivity ...197881212
effect of various nitrogen salts in beet pulp medium on polygalacturonase activity of penicillium sp. 7/4b/ei 1 mutant.the highest pg activity was obtained in beet pulp with (nh4)2hpo4 in amount equivalent to 0.14--0.28% n after 7 days of growth; at optimal concentration, (nh4)2hpo4 did not influence the biomass production, but increased the ph of the culture media to above 6.0. the pg was an extracellular enzyme, acting mainly on highly esterified pectin. the attack was of a random manner, characteristic for the endo-enzymes. the enzyme was active at temperature 20 degrees c--40 degrees c and ph 5.0--6.0.197881597
rapid diagnosis of bacteroides infections by indirect immunofluorescence assay of clinical specimens.43 specimens from a variety of sites were directly examined by indirect immunofluorescence assay (i.f.a.) with specific antisera against the capsular polysaccharide of bacteroides fragilis and pooled antisera against a number of serotypes of bacteroides sp. (all of the former b. fragilis subspecies). the findings were compared with those of routine anaerobic bacteriology and gas liquid chromatography for short chain fatty acids. examination by i.f.a. was a sensitive (100%) and specific (90.3%) m ...197984899
substance p in the vagus nerve. immunochemical and immunohistochemical evidence for axoplasmic transport.1. the presence of immunoreactive substance p (i-sp) in the vagus nerve of 5 species was demonstrated by radioimmunoassay. different amounts of sp per unit weight were found: guinea pig greater than cat greater than rabbit, rat and cattle. 2. infranodose ligations of the vagus nerve of cats and rabbits caused an accumulation of i-sp proximal but not distal to the ligation. the results obtained by radioimmunoassay and immunohistochemistry indicate a somatofugal axoplasmic transport of sp. 3. doub ...197985263
[aspects of the biosynthesis of actinomycin c].the protoplasts of actinomyces sp. 26--115 producing actinomycin c were obtained by the action of lysozyme on the mycelial paste of a 48-hour microbial culture. the protoplast capacity for synthesizing actinomycin was decreased as compared to that of the intact mycelium. the transport of l-isoleucine, a precursor of actinomycin c biosynthesis in the protoplasts also decreased but this could not be the only cause of the decrease in the actinomycin biosynthesis capacity. the biosynthesis of actino ...197987146
ultrastructural localization of sulfated complex carbohydrates with a modified iron diamine procedure.a thiocarbohydrazide-silver proteinate (tch-sp) sequence was applied to thin sections of specimens that had been reacted with the high iron diamine (hid) method for ultrastructural localization of sulfated complex carbohydrates. the exposure to tch-sp enhanced the electron opacity of hid-reactive sites and increased the sensitivity of the procedure. this held true for hid-reacted specimens whether or not they had been post-treated with osmium tetroxide. however in those not postosmicated after h ...197989157
onchocerca sweetae (nematoda: filarioidea): notes on the intermediate host.microfilariae of onchocerca sweetae are broadly distributed in the superficial layers of the dermis of the water buffalo (bubalus bubalis). a total of 2855 insects representing 20 species were collected from o. sweetae-infected bait buffaloes. only one species, culicoides sp. "m", ingested microfilariae from buffalo skin. larval development of o. sweetae was observed in the thorax of this species. atotal of 829 insects, representing 7 species and including 749 parous culicoides spp. were collect ...197826709
[ultrastructure of mycoplasma sp. demonstrated by phosphotungstic acid-chromic acid ph 0,4].ultrastructural observation of mycoplasma sp. revealed by the phosphotungstic acid-cromic acid, ph 0,4. arq. inst. biol., são paulo, 45(3) :169-176, 1978. cells of ib-rs-2 (clone c-26-3) swine line were examined under electron microscopy, employing the differential coloration with pta-acr 5 %, ph 0,4, during 30 minutes, at 37 degrees c. through this method we could observe the intracitoplasmatic forms, electronically dense, rather pleomorfic, with sizes varying between 60nm and 640nm and a unita ...197889840
[isolation of the membranes of actinomyces sp. 26-115, a producer of actinomycin c].a summation fraction of the membranes of actinomyces sp. 26-115 was obtained as a result of lysis of its protoplasts in a hypotonic medium. the qualitative content of protein, lipids, phospholipids, nucleic acids, glucosamine and muramic acid was determined in the membranes at various stages of the organism development. phosphatidylcholine is the main component of phospholipids in this organism. intracellular actinomycin was found inside the protoplasts. electrophoregrams of the microprotoplasts ...197990478
glycoproteins in membranes of secretory granules of the anterior pituitary gland.rat anterior pituitary glands were examined by electron microscopy after staining with five different histochemical stains. histochemical reactions were observed in the cell coat, cell membrane and the membrane surrounding the secretory granules in all anterior pituitary cells following staining with phosphotungstic acid (pta), chromic acid and pta, the periodic acid-thiosemicarbazide-silver protein method (pa-tsc-sp) of thiéry, ruthenium red and concanavalin a. the staining was abolished when t ...197655405
utilization and degradation of lindane by soil microorganisms.of 147 microorganisms isolated from a loamy sand, 71 showed good growth with lindane (gamma-1,2,3,4,5,6-hexachlorocyclohexane) and produced chloride in an aqueous medium. thirteen soil microorganisms were selected to study the utilization of lindane. lindane was metabolized by the microbes to gamma-2,3,4,5,6-pentachloro-1-cyclohexene (gamma-pcch), alpha-3,4,5,6-tetrachloro-1-cyclohexene (alpha-tcch), beta3,4,5,6-tetrachloro-1-cyclohexene (beta-tcch), gamma-3,4,5,6-tetrachloro-1-cyclohexene (gamm ...197660090
substance p infusion into substantia nigra of the rat: behavioural analysis and involvement of striatal dopamine.the purpose of the present study was to characterize the nature of the behavioural response to substance p (sp) infusion into the substantia nigra and to evaluate this response in 6-hydroxydopamine (6-ohda) caudate lesioned rats. the effects of sp infusions (3 microgram in 1 microliter bilaterally) were assessed in an open field. two groups of rats were used: one with 6-ohda lesions in the caudate nucleus, and one with sham lesions. in sham rats, the first infusion produced a strong increase in ...197993550
in vivo release of substance p in the cat substantia nigra.push-pull cannuale were implanted into the substantia nigra (sn) and the caudate nucleus (cn) of the cat to study the in vivo release of substance p (sp), using a radioimmunoassay and high pressure liquid chromatography (hplc) analysis. the spontaneous release of sp could be detected in the sn and the cn. potassium (50 mm) locally applied stimulated sp release in both structures. furthermore an important evoked release of sp was observed in the sn during depolarization of striato-nigral sp fiber ...197993729
bacterial cis-dihydrodiol dehydrogenases: comparison of physicochemical and immunological protperties.cells of pseudomonas putida np, pseudomonas species (ncib 9816), and a nocardia species, after growth on naphthalene as sole source of carbon and energy, contain a nicotinamide adenine dinucleotide (nad+)-dependent enzyme that oxidizes cis-dihydrodiols of mono- and polycyclic aromatic compounds. similarly, cells of a strain of p. putida biotype a, when grown either on toluene or benzene vapors, were found to contain a dehydrogenase that oxidized dihydrodiols of aromatic hydrocarbons with cis ste ...197662750
occurrence and distribution of rhizobiophages in indian soils.soil samples from 30 fields in neighbouring districts of varanasi were screened for rhizobiophages on 60 rhizobium strains. plaques were observed on five strains: p1, p5, su391 (r. leguminosarum), cb756 and 32h1 (rhizobium sp.). rhizobiophages infective on one or more of the five strains were present in all fields. there seems to be no correlation between presence of phage and the standing crop or the ph (7.1-8.2) of the soil. eight distinct rhizobiophages have been isolated and characterized fo ...197994751
[heterogeneity of l-valine pool in actinomyces sp. 26-115 producing actinomycin c].free intracellular valine in actinomyces sp. 26-115 producing actinomycin c its functionally heterogenous. there are at least 2 pools of free valine. one of them supplies valine for protein biosynthesis and the second for the antibiotic biosynthesis. the volume of the pools is estimated. inadequate reaction of the pools to similar effects is indicative of differences in their properties. the pool participating in protein biosynthesis strives for preservation of its volume. the pool participating ...197666908
pyroglutamyl-n3im-methyl-histidyl-prolineamide (methyl-thyrotrophin releasing hormone) in the rat hypothalamus.pyroglutamyl-n3im-methyl-histidyl-prolineamide (methyl-thyrotrophin releasing hormone, methyl-trh) is a potent synthetic analogue of trh. n3im-methyl-histidine is present in mammalian brain and it has been suggested that methyl-trh is a physiological releasing hormone normally present in the hypothalamus. a non-gradient cation-exchange chromatography system that uses sp-sephadex c-25 and completely resolves methyl-trh and trh has been developed. because methyl-trh cross-reacts in the immunoassay ...197896203
formylation and acetylation of 4-chloroaniline by a streptomyces sp.4-chloroaniline was metabolized in a liquid growth medium by a streptomyces sp. which was isolated from soil. after 60 gours of incubation the aniline had disappeared and several metabolites could be detected by thin layer chromatographic analysis. 4-chloroformylaniline and 4-chloroacetanilide were identified as products. the formation of a formylanilide by the actinomycete indicates a new mechanism of microbial aniline transformation.197767755
[effect of a new beta-blocking drug (sp/1601) on the extension of experimental acute infarct in anesthetized dogs]. 197736114
demonstration of intracytoplasmic glycogen of megakaryocytes and blood platelets by means of the periodic acid-thiocarbohydrazide-silver proteinate method.the glycogen of megakaryocytes and blood platelets has been investigated in glutaraldehyde and osmium tetroxide fixed tissues by the periodic acid-thiocarbohydrozide-silver proteinate method (pa-tch-sp). the pa-tch-sp method involves the staining of intracytoplasmic glycogens more densely than the routine lead citrate method. glycogen having a mean particle diameter of 21.1 nm has been shown localizing in the matrix of mature megakaryocytes, while that of glycogen in the platelets was 26.2 nm. t ...197769618
bovine cryptosporidiosis: a transmission and scanning electron microscopic study of some stages in the life cycle and of the host-parasite relationship.transmission and scanning electron microscopy of the ileal mucosae from 12 calves infected with cryptosporidium sp. showed cryptosporidia free in the lumen and attached to epithelium. the attached parasites were interpreted to be extracellular and adherent to the microvillous border of epithelial cells. stages of the organism included crescentic-free merozoites, trophozoites, schizonts, gametes and oocysts. attached parasites were detected chiefly at villous tips and all stages were present on a ...197898896
development and evaluation of an immunodiffusion test for diagnosis of systemic zygomycosis (mucormycosis): preliminary report.an antigen analysis with filtrate and homogenate precipitinogens of single isolates of the zygomycetes absidia corymbifera, mucor pusillus, rhizopus arrhizus, and rhizopus oryzae demonstrated the presence of common antigens among the three genera as well as antigens which permit their differentiation. selected homogenate antigens were valuable in developing a diagnostic immunodiffusion (id) test for systemic zygomycosis. when sera from 43 patients with various proven mycoses other than zygomycos ...197875212
humic-like substances of bacterial origin. i. some aspects of the formation and nature of humic-like substances produced by pseudomonas.two bacterial strains pseudomonas acidovorans no 26 and pseudomonas sp. no 4 grown in conn and yeast extract-glucose media, or in the media enriched with tyrosine, were found to produce dark brown pigment. it was shown that in the bacterial cultures numerous phenolic and quinone-type compounds were formed and transformed to humic-like polymers. formation of humic-like substances started in the bacterial cells and was accompanied by the presence of phenyloxidases in the bacterial cultures. the ba ...197775666
substance p: model studies of its binding to phospholipids.1. the partition of substance p (sp) between buffer solutions (ph 1.6--7.8) and an organic, phospholipid (phosphatidyl serine, phosphatidyl ethanolamine, phosphatidyl inositol and phosphatidyl choline) containing phase (chloroform:methanol 2:1) was studied. 2. the binding of sp to phosphatidyl serine, phosphatidyl ethanolamine and phosphatidyl inositol was lowest at ph 2 and increased with ph. the binding to phosphatidyl choline was much smaller and less dependent on ph. 3. in contrast to the ba ...197938406
purification and some properties of two nadp+-specific isocitrate dehydrogenases from an obligately psychrophilic marine bacterium, vibrio sp., strain abe-1.two isozymes of nadp+-specific isocitrate dehydrogenase [icdh; ec 1.1.1.42] were confirmed to be present in an obligately psychrophilic marine bacterium, vibrio sp., strain abe-1, on the basis of the temperature-activity curve and electrophoretic mobilities. these isozymes were separated and purified about 170-fold for isozyme i (specific activity at 40 degrees c, 24.3 units/mg protein) and about 180-fold for isozyme ii (specific activity at 20 degrees c, 59.2 units/mg protein), though the isozy ...197939069
[allergization of the body by neisseria meningitidis. i. the problem of the occurrence of n. meningitidis in cases of bronchial asthma of bacterial etiology (author's transl)].the object of the authors work was the examination of nasopharyngeal swabs on the prevalence of neisseria meningitidis. a group of 222 patients suffering from asthma bronchiale of bacteriological etiology was under observation in order to be able to prepare an autovaccine. nasopharyngeal swabs were inoculated on special nutrient media. neisseria meningitidis was found in 22%, mainly from nasopharyngeal swabs, together with haemophilus sp. and streptococcus pneumoniae. in 59% of examined cases th ...197899912
physiological and morphological observations on thiovulum sp.cell suspensions of thiovulum sp., collected from enrichment cultures, were grown, maintained, and harvested for periods up to 7 months. in open-flow cultures run with aerated seawater, a continuous supply of hydrogen sulfide was provided by diffusion through a semipermeable membrane from either a live culture of desulfovibrio esturaii, neutralized sodium sulfide, or a n2-h2s gas mixture. attempts to grow thiovulum in pure culture failed despite variation in concentrations of dissolved oxygen an ...1978101531
bacterial decomposition of synthetic 14c-labeled lignin and lignin monomer derivatives.nocardia sp. which was isolated from soil is capable of degrading synthetic lignin and utilizing its monomer derivatives. decomposition was monitored by measuring the 14co2 evolved and o2 consumed, when the bacterium was grown on a medium containing specifically 14c-labeled ligning or monomer phenolic compounds as major carbon source. the time course of the 14co2 release and o2 uptake indicates a significant depolymerization and utilization of lignin by the nocardia sp.197880922
the purification of glutamine synthetase from azotobacter and other procaryotes by blue sepharose chromatography.we report the facile purification of glutamine synthetase (l-glutamate: ammonia ligase (adenosine 5'-diphosphate-forming), ec 6.3.1.2) in both the adenylylated and unadenylylated form, from azotobacter vinelandii atcc 12837. a general affinity column, which used as an affinity ligand reactive blue 2 dye (cibacron blue) covalently linked to agarose, was employed as an efficient first step of purification. further purification to electrophoretic homogeneity employed deae-cellulose chromatography a ...197939606
carbon dioxide assimilation in cyanobacteria: regulation of ribulose, 1,5-bisphosphate carboxylase.cyanobacteria assimilate carbon dioxide through the calvin cycle and therefore must regulate the activity of ribulose 1,5-bisophosphate carboxylase. using an in situ assay, as well as measuring the activity in crude, partially purified, and homogeneous preparations, we can show that a number of phosphorylated intermediates exert a regulatory role. three diverse organisms, agmenellum quadruplicatum, aphanocapsa 6714, and anabaena sp. ca, were studied, and it was found that the in situ and cell-fr ...197940958
quantitative assessment of bactericidal activities of beta-lactam antibiotics by agar plate method.quantitative bactericidal activities of beta-lactam antibiotics were determined by the agar plate method. broth cultures, of which the colony-forming units were counted before the study, were inoculated on antibiotic-containing agar plates, utilizing a 10(-3), 10(-2), or 10(-1) dilution or undiluted culture plated with each 0.001-ml calibrated loop. these plates were incubated at 37 degrees c overnight, and the minimal drug concentration at which no bacterial growth was observed on the plates wa ...1978102246
[epidemiology of leishmaniasis in french guiana in relation to the existence of a new species of echimyid rodent, proechimys cuvieri sp. n].the rediscovery and reexamination of the skull of the type of proechimys guyannensis (e. geoffroy; 1803) allows us to give more details about this species. it appears that a new sympatric species, p. cuvieri, has to be distinguished, which could have great implications for the leishmaniosis epidemiology in french guiana.1978102437
eastern equine encephalomyelitis virus in experimentally infected bats.colonial bats (myotis supp. and eptesicus sp.) were infected with eastern equine encephalomyelitis virus by subcutaneous inoculation or by the bite of infected mosquitoes. bats were maintained in an environment simulating conditions encountered in hibernacula or in summer maternal colonies. virus was detected in the blood of hibernating bats at irregular intervals over a 42-day observation period; viremia perhaps was influenced by the amount of disturbance (arousal) involved in the blood samplin ...197941109
effect of substance p on cck- or vip-induced choleresis in anesthetized dogs.10 anesthetized dogs were provided with acute common bile duct fistulas and the gallbladder was excluded. hepatic bile output and biliary content of sodium, potassium and amylase were studied. 6 caval infusions were administered of cck, 0.3 ivy u.kg-1.min-1, with a superimposed infusion of sp, 20 ng.kg-1.min-1. 7 caval infusions were given of vip, 50 ng.kg-1.min-1, with a superimposed infusion of sp, 20 ng. kg-1.min-1. cck increased bile output and biliary content of sodium, potassium and amylas ...197941410
the incidence of theilerial parasites in east african buffalo (syncerus caffer).245 buffalo from 13 areas of east africa were examined for theilerial infections. the vast majority of buffalo (97.1%) examined had piroplasms in their erythrocytes. theileria lawrencei was isolated from the buffalo by tick feeding and cell culture and was found to be common in most of these buffalo populations. also over 50% of the buffalo had indirect fluorescent antibody (ifa) titres to t. lawrencei. t. mutans was only isolated from 3 buffalo populations but is probably common. haematoxenus s ...1978103262
the parasitic coelenterate, polypodium hydriforme ussov, from the eggs of the american acipenseriform polyodon spathula.no significant differences in macro- and micromorphology were found between the parasitic stolon and free-living polyps of polypodium sp. obtained from infected eggs of the north american acipenseriform fish polyodon spathula and corresponding developmental stages of polypodium hydriforme ussov, parasitic in the volga sterlet (acipenser ruthenus). therefore, both the american and the european forms of polypodium belong to the species p. hydriforme ussov.197941895
human alpha-1-antichymotrypsin: purification and properties.human alpha-1-antichymotrypsin has been purified to homogeneity by the following sequential steps--(a) ammonium sulfate fractionation; (b) chromatography on cibacron blue sepharose at ph 7.0; and (c) chromatography on sp-sephadex c-50 at ph 5.5. the inhibitor has a molecular weight near 68,000 and contains approximately 26% carbohydrate alpha-1-antichymotrypsin has an amino-terminal arginine and a carboxy-terminal glycine. it also has some homology with alpha-1-pi based on amino-terminal sequenc ...1978103576
[efficacy, tolerance, and pharmacokinetics of sisomicin in urinary tract infections (author's transl)].since 1973 30 patients with urinary tract infections (uti) or pyelonephritis have been treated with sisomicin, a new aminoglycoside, in a daily dose of 2 mg/kg for a period of seven to nine days. from a clinical point of view the result of treatment was good. complete resolution was achieved in 17 patients, improvement in nine, and there was no effect in four patients. thirty-five causative organisms (escherichia coli = 23, proteus sp. = 7, klebsiella sp. = 3, pseudomonas aeruginosa = 1, citroba ...1978103844
isolation and properties of the protease from the wild-type and mutant strains of pseudomonas fragi.a simplified procedure for the purification of the extracellular protease of pseudomonas fragi was developed. the enzyme was isolated from a derepressed mutant producing 40 times the enzyme level of the parental organism. it was collected from culture filtrates by ammonium sulfate precipitation, and it was obtained in pure form by single chromatography on a column of diethylaminoethyl cellulose. the protease had a molecular weight of 52,000 as estimated by sodium dodecyl sulfate-gel electrophore ...197942639
studies on acute and late stages of experimental central retinal artery occlusion in the cynomolgus monkey. ii. influence on the cyclic changes in the amplitude of the c-wave of the erg and in the standing potential of the eye.the slow (2-3/h) oscillations of the c-wave amplitude and of the standing potential of the eye (sp) were studied in the cynomolgus monkey during the acute and late stages after experimentally induced (laser photocoagulation) occlusion of the central retinal artery (ocra). whereas the healthy control eyes showed large cyclic variations in both the c-wave amplitude and the sp, no oscillations in the c-wave amplitude were observed in the ocra eyes at any stage, and the sp oscillations were barely d ...1978104543
the femur in early human evolution.uni- and multivariate analyses of 5 fossil and 215 extant hominoid femora show that two morphological patterns of hominid femora existed about two million years ago. femora classified as homo sp. indet. (knmer 1472 and 1481) are more like homo sapiens although not identical. those classified as australopithecus robustus (sk 82 and 97) and a. boisei (knm-er 1503) are similar to one another but uniquely different from any living hominoid. the strong mophological constrasts imply biomechanical and ...1978104627
cc-1065 (nsc-298223), a new antitumor antibiotic. production, in vitro biological activity, microbiological assays and taxonomy of the producing microorganism.a new antitumor antibiotic is produced in fermentation liquors of streptomyces zelensis sp.n. the antibiotic is biologically active at extremely low concentrations. at 40 pg/ml, it inhibited 90% of the growth of l1210 cells in culture in tube dilution assays. the minimal inhibitory concentrations against gram-positive bacteria is between 1 approximately 10 ng/ml, while these values for gram-negative bacteria and fungi are mostly under 1 microgram/ml. a microbiological assay with bacillus subtili ...1978104946
homology of the gene coding for outer membrane lipoprotein within various gram-negative bacteria.the mrna for a major outer membrane lipoprotein from escherichia coli was found to hybridize specifically with one of the ecori and one of the hindiii restriction endonuclease-generated fragments of total dna from nine bacteria in the family enterobacteriaceae: e. coli, shigella dysenteriae, salmonella typhimurium, citrobacter freundii, klebsiella aerogenes, enterobacter aerogenes, edwardsiella tarda, serratia marcescens, and erwinia amylovora. however, among the enterobacteriaceae, dna from two ...1979104972
serious infections from bacillus sp.serious infections caused by organisms of the genus bacillus developed in seven patients. five drug abusers had either endocarditis or osteomyelitis, one leukemic patient had necrotizing fasciitis, and one patient had a ventriculoatrial shunt infection with recurrent bacteremia. all patients recovered. experience with these cases reemphasizes the importance of not dismissing bacillus organisms as culture contaminants, especially when isolated from blood, body fluids, or closed-space infections.1979105158
sarcocystis sp from the goat in india. 1978105455
azo reduction of trypan blue to a known carcinogen by a cell-free extract of a human intestinal anaerobe.the azo reductase activity of a cell-free extract of fusobacterium sp. 2 is characterized using trypan blue as a substrate. either chemical reduction of this dye with sodium hydrosulfite or reduction by the cell-free extract produces a mutagenic product, o-tolidine. the o-tolidine is mutagenic in the ames salmonella/mammalian-microsome mutagenicity test when activated by a rat liver s9 preparation.1978106267
[monitoring the helmintological situation in a large-scale reproduction of pigs].in 1976 to 1978, the occurrence of helminths in a large reproduction herd of pigs was studied in different age categories of the animals kept. six helminth species were found in 30.7% of the sows: ascaris suum (6.6%), oesophagostomum dentatum (24.0%), hyostrongylus rubidus (3.3%), trichocephalus suis (1.3%), strongyloides ransomi (0.7%), metastrongylus sp. (0.3%). four- to seven-month-old auction gilts had only the eggs of a. suum (4% of all cases). no helminith eggs were found in fattened pigle ...1979106504
a novel tetrahymena sp. with unusual membrane lipid composition, with special reference to iso fatty acyl chains.a novel tetrahymena sp. is similar in appearance to four classical strains (wh-14, gl, w, nt-1) of t. pyriformis except for a larger width. the phospholipid and acyl chain compositions of membranes of this novel species are significantly different from those of other strains. the tetrahymena sp. has a much higher content of ethanolamine phosphoglyceride and a lower level of glyceroaminoethyl phosphonate. major fatty acids of this strain are iso 15:0 and iso 17:1, which are, if present, usually m ...1978107352
localization of hypophysiotropic peptides and other biologically active peptides within the brain.immunohistochemical analysis of hypophysiotropic and other neuropeptides reveals unique and striking neural perikarya, axons, and terminals containing specific peptide immunoreactivity. the hypophysiotropic peptides are most highly concentrated in nerve terminals in the external layer of the median eminence. from this site they may be released and carried via the portal circulation to the adenohypophysis. the occurrence of hypophysiotropic peptides in other areas of the nervous system suggests t ...1979107852
[new species of microsporidians (microsporidia, nosematidae) from horsefly larvae in the ukraine].a new species of microsporidians, stempellia lairdi sp. n., was found in larvae of tabanus autumnalis l. from ukraine. it affects the muscular tissue and haemolymph of horse fly larvae. spores of the species are oval, egg-shaped, 1.8--7.6 x 1.2--3.8 mu in size. the species is pathogenic and causes the mortality of infected larvae.1979108652
[new species of parasitic infusoria of the family urceolariidae].three new species of ciliata of the family urceolariidae were found as follows: paratrichodina voikarensis sp. n. on the gills of coregonus nasus and c. peled; p. uralensis sp. n. on the gills of the sterlet and trichodina percae sp. n. in the nasal cavities of the perch. figures and descriptions of the new species are given.1979108655
decomposition of ddt and its analogs by soil microflora.a search was made for active cultures that break down ddt. more than 600 microorganisms were isolated from soils treated for a long time with ddt and from accumulating cultures with ddt or its analogs as the carbon source and with ddt or its analogs and supplementary carbon sources. cultures capable of degrading ddt under conditions of cometabolism were found among them. it was shown that the dechlorination of the trichloromethyl group of ddt was accomplished by a large number of microorganisms ...1978109130
hematozoa from southern african vultures, with a description of haemoproteus janovyi sp. n.this study represents the first major survey of avian hematozoa from southern africa and the only one dealing with blood parasites of vultures. blood smears from 506 rhodesian, botswanan and south african vultures (hooded, white-headed, lappetfaced, cape griffon and whitebacked vultures) were examined for hematozoa. haemoproteus janovyi sp. n. was observed in 35.2% of the vultures, leucocytozoon toddi in 0.8%, plasmodium fallax in 0.6%, atoxoplasma sp. in 1.4% and microfilariae in 0.2%. hematozo ...1979109605
rocky mountain spotted fever in connecticut: human cases, spotted-fever group rickettsiae in ticks, and antibodies in mammals.three parameters were used in 1976 and 1977 to assess the status of rocky mountain spotted fever (rmsf) in connecticut--compilation and review of clinical data on suspected human cases for the 13-year period 1965--1977, examination of tick tissues for spotted fever-group rickettsiae by the hemolymph test and direct immunofluorescence, and analyses of mammalian sera for antibodies against rickettsia rickettsii. there were six presumptive rmsf cases which probably originated in connecticut. four o ...1979111543
light and electron microstructure of a sarcocystis sp. from the malaysian long-tailed monkey, macaca fascicularis.the ultrastructure of the cyst wall and zoites of a species of sarcocystis from the skeletal muscles of a naturally-infected malaysian long-tailed monkey, macaca fascicularis, is described in detail. the wavy, electron-dense primary cyst wall is thin (55 nm) and invaginated. cytophaneres are absent. the ground substance contains electron-dense granules and bundles of parallel, fibrillar elements in some areas. thin trabeculae are present. the zoites measure 1.2 x 4.7 microns and have an interior ...1979111569
dihydrofolate reductase from neisseria sp.members of the genus neisseria are relatively nonsusceptible to trimethoprim, an inhibitor of dihydrofolate reductase. for example, the minimal inhibitory concentration (mic) of trimethoprim for n. gonorrhoeae ranges from 2 to 70 mug/ml, whereas the mic for escherichia coli is 0.2 mug/ml or less. in an effort to understand this difference, dihydrofolate reductase was partially purified from five neisseria species and compared with the enzyme from e. coli. n. gonorrhoeae dihydrofolate reductase w ...1979111611
[action of aspergillus sp. on trypanosoma cruzi in vitro]. 1979112665
identification of colonies of moraxella bovis by plate epi-immunofluorescence.a modification of the plate epi-immunofluorescence test was found to be a reliable method for the positive identification of colonies of moraxella bovis when fluorescein isothiocyanate-conjugated globulin prepared against pili from selected strains of m bovis was used. a cross-reaction with other members of the genus moraxella or other moraxella-like organisms was not observed, except with an equine moraxella sp, which produced a weak reaction.197943106
a comparison of the in vitro activity of tobramycin and gentamicin against 6,042 clinical isolates.6,042 strains of staphylococcus aureus, streptococcus faecalis, proteus mirabilis, escherichia coli, klebsiella sp. and pseudomonas sp. were tested against tobramycin and gentamicin. among all species studies, except pseudomonas sp, gentamicin turned out to be slightly superior to tobramycin.1979113179
herpesvirus papio: state and properties of intracellular viral dna in baboon lymphoblastoid cell lines.herpesvirus papio (hvp) is an indigenous b-lymphotropic virus of baboons (papio sp.) present in latent form in baboon lymphoblastoid cell lines. it shares cross-reacting viral capsid and early antigens with the epstein-barr virus (ebv), and hvp dna and ebv dna show partial sequence homology. ebv-specific complementary rna was employed here as a probe to investigate the physical state of the hvp dna component in baboon lymphoblastoid cells after fractionation of cellular dna by density gradient c ...1979113352
inhibition of the in vitro growth of plasmodium falciparum. i. the effects of immune serum and purified immunoglobulin from owl monkeys.sera from aotus sp. monkeys (karyotypes ii, iii, and iv) which were immune to plasmodium falciparum have been used to inhibit the in vitro growth of this human malaria parasite. culture conditions used for the assays allowed 50- to 100-fold increases in the number of a+ erythrocytes infected in a 96-hr period in control cultures. although normal monkey serum did not support growth as well as normal human serum, mixtures of normal monkey and human serum were found that did. compared to such contr ...1979113460
parasite-related lesions in a bearded seal, erignathus barbatus.a free-ranging adult male bearded seal (erignathus barbatus) killed by polar bears was salvaged and examined at necropsy. significant findings included diffuse intrahepatic bile duct fibrosis and chronic cholangitis; multiple nodules of chronic fibrosing pancreatitis; and gastric ulcers. trematode eggs (family campulidae) were found in the pancreatic nodules. these eggs and the trematodes that produced them probably caused the pancreatic and liver lesions. phocanema decipiens and contracaecum os ...1979113564
bioelectric phenomena in the ototoxicity of nitrogen mustard.the effects of nitrogen mustard on the electrical potentials of the inner ear were studied, and the results were correlated with the histopathologic findings which have been reported in nitrogen mustard ototoxicity. the endocochlear dc potential (ep) decreased rapidly after an injection of nitrogen mustard (nm). the amplitude of the cochlear microphonics potential (cm) diminished rapidly, and no substantial recoveries were observed. no significant changes in the magnitude of the negative potenti ...1978113748
patterns of variability in mineralization of the primate femoral diaphysis.the purpose of this research was to study intra- and interspecific variability in mineral density in the femoral diaphysis of nonhuman primates. four hundred five sections were taken from five sites along the femoral diaphysis of 34 macaques (macaca sp.), 24 squirrel monkeys (saimirisciureus), and 23 tamarins (saguinus labiatus). the mineral density at eight positions around each section was measured. analysis of variance indicated significant (p less than 0.05) interactions among species, sex, ...1979114060
macromolecular composition of a cellulomonas sp. cultivated in continuous culture under glucose and zinc limitation.the mutant strain of cellulomonas sp. (atcc 21399) was cultivated under glucose and zinc limitation at a variety of growth rates in continuous culture. the growth characteristics and macromolecular composition of the population varied with the limitation imposed and the growth rate. glucose- and zinc-limited cultures maintained a constant relative protein content. the relative ribonucleic acid content increased, whereas the carbohydrate and deoxyribonucleic acid contents decreased with an increa ...1979114114
a mutant of anabaena sp. ca with oxygen-sensitive nitrogenase activity. 1979114179
[opportunistic fungi in the atmosphere].our knowledge in aerobiology has considerably increased in the last 20 years due to new trapping methods. the use of volumetric spore traps permits to accurately analyze the particles suspended in the air and their evolution, while the deposition method gives an incomplete picture of the real air content. the air microflora changes considerably during 24 h: some groups of spores occur in the morning, others in the afternoon and yeast-like organisms during the night. the most common fungi in the ...1979114434
strain-specific antigens in rhizobium leguminosarum.fifty strains of rhizobium leguminosarum, isolated from five species of host plant (pisum sativum, p. arvense, vicia sativa, v. faba, and a lathyrus sp.) were examined for the presence of strain-specific somatic antigens by immune-diffusions against 13 antisera. thirty eight strains (76 per cent) were found to belong to the same sero-group and were serologically indistinguishable from each other, but four of these strains also exhibited non-reciprocal cross reactivity with other antisera. in con ...197989766
surveys of simian malaria in japan.the survey of simian malaria in japan which had been conducted since 1965 showed that no japanese monkeys (macaca fuscata fuscata and macaca fuscata yakui) were infected with malaria parasites. most interestingly, however, babesia sp. was found in 4 (4.3%) of 93 macaca fuscata yakui which had been reared in a monkey park. as for the monkeys imported from abroad, plasmodium was only found in macaca irus from southeast asia, namely, p. inui (14.1-25.0%), p. cynomologi (0.6, 3.2%) and p. coatneyi ( ...1979116437
nitrogenase activity of rhizobium sp. strains in pure culture in relation to extracellular polysaccharide composition and antigenic affinity.five strains of slow-growing rhizobium sp. (strains cb756, 32hi, cb562, cb627 and qa549) out of seventy examined developed appreciable nitrogenase activity in pure culture. cb756 and 32hi were serologically indistinguishable and each produced 6-deoxy-l-talose as a major component of its extracellular polysaccharide. they did not share these properties with cb562, cb627 or qa549.1978117284
brain trf immunoreactivity during various physiological and stress conditions in the rat.methanol extracts of several rat tissues (hypothalamus, amygdala, the rest of the forebrain, brain stem and pancreas) were partially purified in sp-cation exchange chromatography and measured in trf radioimmunoassay. it was found that hypothalamic and amygdaloid trf contents were highest during the light period of the day, but brain stem trf had an opposite rhythm. hypothalamic trf content rose after 10 min by 14% (p less than 0.05) when the rats were exposed to transfer stress. during short-tim ...1979117391
acid hydrolases of trypanosomatid flagellates.homogenates from 5 species of trypanosomatids were screened for the presence of a series of acid hydrolases. the insect flagellae, crithidia sp., contains 5 enzymes reminiscent of plant parasitism, which were absent from or of very low specific activity in parasites of the genera, trypanosoma and leishmania. the latter mammalian parasites, on the other hand, exhibited higher acid proteinase and alpha-d-mannosidase activity levels.197944097
iso-branched 2- and 3-hydroxy fatty acids as characteristic lipid constituents of some gliding bacteria.the fatty acids present in the total hydrolysates of several gliding bacteria (myxococcus fulvus, stigmatella aurantiaca, cytophaga johnsonae, cytophaga sp. strain samoa and flexibacter elegans) were analyzed by combined gas-liquid chromatography and mass spectrometry. in addition to 13-methyl-tetradecanoic acid, 15-methyl-hexadecanoic acid, hexadecanoic acid, and hexadecenoic acid, 2- and 3-hydroxy fatty acids comprised up to 50% of the total fatty acids. the majority was odd-numbered and iso-b ...1979118159
parasites in bulinus senegalensis (mollusca: planorbidae) and their detection.isoelectric focusing studies on enzyme variation between populations of the snail bulinus senegalensis revealed that parasitic infections in the snails contributed additional bands of enzyme activity, particularly in the glucose phosphate isomerase (gpi) and malate dehydrogenase (mdh) systems. the patterns due to the parasite enzymes were, in most cases, clearly distinct from those of the host and different from each other. parasites encountered included schistosoma haematobium, s. bovis, paramp ...197944358
the automicrobic system for urines.an evaluation of the automicrobic system (ams) for urines (vitek systems, inc.) was carried out under the auspices of the product evaluation committee of the college of american pathologists from the period june 1977 through october 1978. data generated during this evaluation indicated that, when comparing the ams methodology to our clinical microbiology laboratory methodology, a 37% time saving could be realized by utilizing the ams. quantitation with the ams showed a 99% correlation with the c ...1979118176
comparative bactericidal activities of beta-lactam antibiotics determined in agar and broth media.comparative bactericidal activities were determined utilizing a relatively large number of test strains, in both agar and broth media, with special reference to the time of exposure of the bacteria to certain beta-lactam antibiotics. it was apparent that the activities increase with time. the concentrations producing a 99.9% kill with cephalothin for escherichia coli, klebsiella sp., and carbenicillin for pseudomonas aeruginosa were higher in broth than in agar. in contrast, those of benzylpenic ...1979118959
cadmium and zinc sensitivity and tolerance in bacillus subtilis subsp. niger and in a pseudomonas sp.the action of cd2+ and zn2+ on bacillus subtilis subsp. niger atcc 9372, and on a pseudomonas sp. (possibly pseudomonas fluorescens), isolated from cadmium-polluted soil has been determined and compared with results obtained previously with klebsiella aerogenes. in liquid medium the lag and the mean generation time of bacillus subtilis subsp, niger increased with increasing cd2+ or zn2+ concentrations whereas only the total biomass of the pseudomonas sp. was affected. nevertheless, the responses ...1979119131
the role of temperate bacteriophage sp beta in prophage-mediated interference in bacillus subtilis.virulent bacteriophage phi 1 grows on a variety of bacillus subtilis strains, mutants of this virus which abortively infect the transformable bacillus. b. subtilis 168, while retaining the ability to productively infect related bacteria have been found. in the present study, we demonstrate that the inability of one such variant, phi 1m, to develop normally in strain 168 is mediated by cryptic prophage sp beta. the latter is a temperate bacteriophage which is carried by b. subtilis 168 and most s ...1979119575
studies on the role of calcium ions in acetylene reduction (nitrogen fixation) by gloeocapsa sp. 1430/3 [proceedings]. 1979119655
an injury-induced diffuse slow potential from brain.three different slow potential (sp) changes resulting from focal brain injury are described. the first is an immediate, high amplitude (in excess of 25 mv)negative shift at the site of injury. the second is a biphasic negative-positive sp wave which spreads throughout the cortex ipsilateral to injury and is similar to spreading depression (sd). the third sp change, called here the injury-induced diffuse slow potential (idsp)is a prolonged (lasting approx. 2h) negative shift occurring simultaneou ...197546817
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