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respiratory symptoms, immunological responses, and aeroallergen concentrations at a sawmill.after identification of a case of extrinsic allergic alveolitis due to exposure to wood dust at a sawmill, all employees at the sawmill where he worked were studied with an occupational, environmental, and symptom questionnaire, spirometry, skin prick tests, and serum specific igg measurements. ninety five of current and 14 of 17 ex-sawmill workers were studied. as a basis for comparison, a group of 58 workers from a nearby light engineering factory were also studied. few women (6) were employed ...19948130844
extrinsic allergic alveolitis and asthma in a sawmill worker: case report and review of the literature.a 34 year old sawmill maintenance engineer developed a dry cough that was associated with widespread wheezes and crackles in his lungs. his symptoms worsened, with work related lethargy, fever, and breathlessness, and the loss of a stone in weight. at that time, while still at work, he had a neutrophil leucocytosis and increased concentration of gamma globulins. when seen subsequently some two months after stopping work, his chest radiograph and lung function tests were normal, but the cells rec ...19948130843
[purification and properties of low molecular weight endoglucanase of the cellulase complex from trichoderma koningii].a homogenous low molecular weight 1,4-beta-glucan glucanohydrolase (endoglucanase) has been isolated from a crude commercial preparation of cellokoningine p10x of t. koningii origin. the molecular weight of the enzyme as determined by polyacrylamide gel electrophoresis is 13 000. the endoglucanase was obtained as a lyophylized preparation free of the cellobiase activity. it was shown that cellobiose or methylcellobioside activate the effect of the low molecular weight endoglucanase (measured by ...19807189672
two glyceraldehyde-3-phosphate dehydrogenase isozymes from the koningic acid (heptelidic acid) producer trichoderma koningii.the sesquiterpene lactone koningic acid (heptelidic acid) irreversibly inactivated glyceraldehyde-3-phosphate dehydrogenase [d-glyceraldehyde 3-phosphate: nad+ oxidoreductase (phosphorylating)] (ec 1.2.1.12) (gapdh) and thus inhibits glycolysis. the koningic-acid-producing strain of trichoderma koningii m3947 was shown to contain the koningic-acid-resistant gapdh isozyme (gapdh i) under conditions of koningic acid production. in peptone-rich medium, however, no koningic acid production was obser ...19902226438
rna interference with carbon catabolite repression in trichoderma koningii for enhancing cellulase production.the cellulase and xylanase genes of filamentous trichoderma fungi exist under carbon catabolite repression mediated by the regulator carbon catabolite repressor (crei). our objective was to find the role of crei in a cellulase-hyperproducing mutant of trichoderma koningii, and address whether enzyme production can be further improved by silencing the cre1 gene. cre1 partially silenced strains were constructed to improve enzyme production in t. koningii yc01, a cellulase-hyperproducing mutant. si ...201323769310
cellulase production and saccharification of rice straw by the mutant strain hypocrea koningii rsc1.the production of cellulase using solid-state fermentation of rice straw by the mutant strain hypocrea koningii rsc1 was studied. optimization of culture conditions, such as the nitrogen source, ph, and temperature, resulted in a maximum filter paper cellulase activity of 44.15 u g(-1) substrate, a carboxymethylcellulase activity of 324.6 u g(-1) substrate, and a β-glucosidase activity of 7.45 u g(-1) substrate. saccharification of untreated, 1% h(2)so(4)-treated, and 2.5% naoh-treated rice stra ...201423775722
trichoderma songyi sp. nov., a new species associated with the pine mushroom (tricholoma matsutake).a new species, trichoderma songyi, was found to be associated with the pine mushroom (tricholoma matsutake) in korea. this species was isolated from three different substrates: tricholoma matsutake basidiomata, as well as roots of pinus densiflora and soil in the fairy ring. based on its molecular and phenotypic characteristics, we demonstrate that trichoderma songyi is unique and distinguishable from closely related species. we performed phylogenetic analyses based on two molecular markers, the ...201425052534
new furan and cyclopentenone derivatives from the sponge-associated fungus hypocrea koningii pf04.two new furan derivatives, hypofurans a and b (1 and 2), and three new cyclopentenone derivatives, hypocrenones a-c (3-5), along with seven known compounds (6-12), were isolated from a marine fungus hypocrea koningii pf04 associated with the sponge phakellia fusca. among them, compounds 10 and 11 were obtained for the first time as natural products. the planar structures of compounds 1-5 were elucidated by analysis of their spectroscopic data. meanwhile, the absolute configuration of 1 was deter ...201526343687
effect of fungi and light on seed germination of three opuntia species from semiarid lands of central mexico.fungal attack under light reduces mechanical resistance of the testa of opuntia seeds, making it easier for the embryo to emerge. however, the effect of fungi on opuntia seed germination in darkness is unknown. we evaluated the combined effects of light and inoculation with phoma medicaginis, trichoderma harzianum, trichoderma koningii, and penicillium chrysogenum on germination of o. streptacantha, o. leucotricha, and o. robusta seeds, from central mexico. we also evaluated the combined effects ...201323526153
cunninghamella echinulata a new biosorbent of metal ions from polluted water in egypt.thirty-two fungal species were isolated from a polluted watercourse near the talkha fertilizer plant, mansoura province, egypt. aspergillus niger, a. flavus, cunninghamella echinulata and trichoderma koningii were isolated frequently. on the basis of its frequency, cunninghamella echinulata was chosen for biosorption studies. free and immobilized biomass of c. echinulata sequestered ions in this decreasing sequence is: pb >cu >zn. the effects of biomass concentration, ph and time of contact were ...200421148940
trichodermaketones a-d and 7-o-methylkoninginin d from the marine fungus trichoderma koningii.five new polyketide derivatives, 7-o-methylkoninginin d (1) and trichodermaketones a-d (2-5), together with four known compounds, koninginins a, d, e, and f, were isolated from the marine-derived fungus trichoderma koningii. trichodermaketones a (2) and b (3) are unprecedented polyketides with a bistetrafuran-containing tricyclic skeleton. the chemical structures and absolute configurations of compounds 1-5 were elucidated by comparing with literature data and extensive spectroscopic methods, in ...201020384316
[study on conformation transitions of trichokonin vi, a peptaibol-like antimicrobial peptide, in different solvents by circular dichroism spectroscopy].trichokonin vi, a peptaibol-like antimicrobial peptides isolated from the cultured substrates of trichoderma koningii smf2, has 20 amino acid residues. the conformational flexibility of trichokonin vi in organic solvents with different polarities, aqueous solvents and membrane mimic solvents was studied by circular dichroism spectroscopy. trichokonin vi takes on a typical alpha-helical structure in different organic solvents, but helicity decreases in aqueous solvent. the helical content increas ...201020384145
soil chemical and physical properties associated with suppression of take-all of wheat by trichoderma koningii.abstract trichoderma koningii, originally isolated from a take-all-suppressive soil in western australia, has been shown to protect wheat against take-all disease and increase grain yield in field trials in australia, china, and the united states. however, within a region, the level of protection provided by t. koningii can dramatically vary between field sites. we evaluated suppression of take-all by this fungus in eight silt loams from the pacific northwest of the united states and the influen ...199718945008
the trichoderma koningii aggregate species.the morphological concept of trichoderma koningii is found to include several species that differ from each other in details of phenotype (including conidium morphology, growth rate) and biogeography. phylogenetic analysis utilizing partial sequences of the translation-elongation factor 1 alpha (tef1), as well as fragments of actin and calmodulin genes, indicate that phenotypic characters typical of t. koningii evolved independently in three well-separated main lineages. combined molecular and p ...200618490990
efficient biodegradation of cyanide and ferrocyanide by na-alginate beads immobilized with fungal cells of trichoderma koningii.cyanide or metal cyanide contaminations have become serious environmental and food-health problems. a fungal mutant of trichoderma koningii, tka8, constructed by restriction enzyme-mediated integration, has been verified to have a high cyanide degradation ability in our previous study. in this study, the mutant cells were entrapped in sodium-alginate (na-alginate) immobilization beads to degrade cyanide and ferrocyanide in a liquid mineral medium. the results showed that the fungus in immobiliza ...200718026223
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