| the replicon of psw800 from pantoea stewartii. | a 2019 bp dna fragment containing the replicon of psw800 from pantoea stewartii sw2 was cloned and characterized. this replicon contains two genes--repa and repb, which encode a 36.5 kda replication initiation protein (repa) and a peptide of 18 aa, respectively. these two genes overlap by 8 bases with repb situated upstream. the replicon also transcribes an antisense rna (rnai) that inhibits the expression of repa and repb. the ribosome-binding sequence (rbs) of repa is likely to be hidden in a ... | 2001 | 11577155 |
| genetic organization of the pantoea stewartii subsp. stewartii hrp gene cluster and sequence analysis of the hrpa, hrpc, hrpn, and wtse operons. | the hrp/wts gene cluster of pantoea stewartii subsp. stewartii is required for pathogenicity on sweet corn and the ability to elicit a hypersensitive response (hr) in tobacco. site-directed transposon mutagenesis and nucleotide sequencing were used to identify hrp/wts genes within the left 20 kb of this cluster. seventeen open reading frames (orfs) comprise seven genetic complementation groups. these orfs share homology with hrp and dsp genes from erwinia amylovora, erwinia chrysanthemi, and pse ... | 2001 | 11605961 |
| biological activity of harpin produced by pantoea stewartii subsp. stewartii. | pantoea stewartii subsp. stewartii causes stewart's wilt of sweet corn. a hypersensitive response and pathogenicity (hrp) secretion system is needed to produce water-soaking and wilting symptoms in corn and to cause a hypersensitive response (hr) in tobacco. sequencing of the hrp cluster revealed a putative harpin gene, hrpn. the product of this gene was overexpressed in escherichia coli and shown to elicit the hr in tobacco and systemic resistance in radishes. the protein was designated hrpn(pn ... | 2001 | 11605962 |
| crystallization and rhenium mad phasing of the acyl-homoserinelactone synthase esai. | acyl-homoserine-l-lactones (ahls) are diffusible chemical signals that are required for virulence of many gram-negative bacteria. ahls are produced by ahl synthases from two substrates, s-adenosyl-l-methionine and acyl-acyl carrier protein. the ahl synthase esai, which is homologous to the ahl synthases from other pathogenic bacterial species, has been crystallized in the primitive tetragonal space group p4(3), with unit-cell parameters a = b = 66.40, c = 47.33 a. the structure was solved by mul ... | 2001 | 11717525 |
| microfloral diversity of cultured and wild strains of psoroptes ovis infesting sheep. | pcr amplification of 16s rdna was used to determine the diversity of bacteria associated with 3 strains of sheep scab mite, psoroptes ovis. eight species of bacteria were identified by phylogenetic analysis of the pcr product sequences. seven of these species are previously unreported in association with sheep scab mites. five species were matched to serratia marcesens, proponibacteium acnes, phyllobacterium rubiacearum, pantoea agglomerans, curacaobacter baltica, whereas the remaining 3 sequenc ... | 2001 | 11719954 |
| young farmers with cellular reactivity to airborne microbes suffer more frequently from work-related skin symptoms and allergic dermatitis. | 75 farming students (49 males and 26 females aged 16-23 years) underwent dermatological, laryngological and pulmonary examination, skin prick tests with common and farm allergens, phadiatop and total ige measurement. after that, the migration inhibition tests with antigens of airborne microbes typical for farm environment (saccharopolyspora rectivirgula, pantoea agglomerans, and aspergillus fumigatus) were carried out. possible differences between students with positive results and those non-rea ... | 2001 | 11748885 |
| response of herb processing workers to work-related airborne allergens. | a group of 51 herb processing workers employed in a big herb processing facility located in eastern poland were examined by the skin and precipitin tests with, respectively, 4 and 17 extracts of microorganisms associated with organic dusts. out of this number, 32 workers were examined by the skin test with 7 extracts of selected herbs processed in the facility. all the subjects were asked about the occurrence of work-related symptoms. 32 healthy office workers were examined with microbial extrac ... | 2001 | 11748888 |
| biological control of postharvest pear diseases using a bacterium, pantoea agglomerans cpa-2. | epiphytic microorganisms isolated from the fruits and leaf surfaces of apples and pears were screened for antagonistic activity against penicillium expansum on pears. from 247 microorganisms tested for antagonistic properties against p. expansum, a bacterium strain identified as pantoea agglomerans (cpa-2) was selected. this bacterium was very effective against botrytis cinerea, p. expansum and rhizopus stolonifer. complete control at the three tested concentrations (2 x 10(7), 8 x 10(7) and 1 x ... | 2001 | 11759762 |
| control of penicillium expansum and botrytis cinerea on apples and pears with the combination of candida sake and pantoea agglomerans. | the effectiveness of candida sake (cpa-1) in combination with pantoea agglomerans (cpa-2) for controlling penicillium expansum and botrytis cinerea on pears and apples was determined. the concentrations tested were 2 x 10(6) and 2 x 10(7) cfu/ml for c. sake and 2 x 10(7) and 8 x 10(7) cfu/ml for p. agglomerans. at room temperature, the two antagonists were combined in proportions of 0 to 100% in 25% increments. at the proportion of 50:50, no rot development was observed in pears, and the greates ... | 2002 | 11808791 |
| survival of pantoea agglomerans strain cpa-2 in a spray-drying process. | spray drying could be a suitable method for preserving microorganisms, as it allows large quantities of cultures to be dried at low cost. the aims of this paper were to evaluate the effects of spray-drying conditions on survival of the biocontrol agent pantoea agglomerans cpa-2, which has shown antifungal activity against penicillium expansum and penicillium digitatum on citrus fruits. various compounds cited in the bibliography as carriers were tested in our spray drying, and some salts (mgso4, ... | 2002 | 11808793 |
| occurrence and transferability of beta-lactam resistance in enterobacteriaceae isolated in children's university hospital in bratislava. | occurrence and transferability of beta-lactam resistance in 30 multi-resistant escherichia coli, klebsiella spp., enterobacter spp., pantoea agglomerans, citrobacter freundii and serratia marcescens strains isolated from children between 0 and 3 years of age is presented. the strains were resistant to ampicillin (30), cefoxitin (22), cefotaxime (30), ceftriaxone (30), ceftazidime (30) and aztreonam (28), but susceptible to cefepime (30) and imipenem (26). twenty-eight of 30 isolates possessed a ... | 2001 | 11830947 |
| posttranscriptional activation of the transcriptional activator rob by dipyridyl in escherichia coli. | the transcriptional activator rob consists of an n-terminal domain (ntd) of 120 amino acids responsible for dna binding and promoter activation and a c-terminal domain (ctd) of 169 amino acids of unknown function. although several thousand molecules of rob are normally present per escherichia coli cell, they activate promoters of the rob regulon poorly. we report here that in cells treated with either 2,2"- or 4,4"-dipyridyl (the latter is not a metal chelator), rob-mediated transcription of var ... | 2002 | 11844771 |
| post-harvest biological control by pantoea agglomerans (cpa-2) on golden delicious apples. | to investigate the potential of pantoea agglomerans to control the major post-harvest diseases on golden delicious apples. | 2002 | 11849352 |
| a note: gut bacteria produce components of a locust cohesion pheromone. | faecal pellets from germ-free locusts were used as culture media to determine the ability of locust gut bacteria to synthesize phenolic components of the locust cohesion pheromone. | 2002 | 11966918 |
| effect of package and storage conditions on viability and efficacy of the freeze-dried biocontrol agent pantoea agglomerans strain cpa-2. | to reduce concentrations of protective and rehydrating media and to evaluate the effect of storage temperature, packaging and atmosphere conditions on the stability of freeze-dried pantoea agglomerans cells. efficacy against penicillium digitatum of freeze-dried cells in orange fruits was also evaluated. | 2002 | 11972691 |
| functional analysis of the erwinia herbicola tutb gene and its product. | the tutb gene, which lies just downstream of tpl, has been cloned from erwinia herbicola, and its product was analyzed. despite its high sequence similarity to tryptophan transporters, tutb was found to be a tyrosine-specific transporter. tryptophan acted as a competitive inhibitor of tyrosine transport. unlike the tryptophanase operon, the tpl and tutb genes do not constitute an operon. | 2002 | 12003958 |
| response of the ice-nucleating bacterium pantoea ananas kuin-3 during cold acclimation. | the ice-nucleating bacterium pantoea ananas kuin-3 accumulated glucose in cells following a shift in temperature (10 degrees c) from the optimum growth temperature (30 degrees c). this accumulation might be caused by the activation of glucose-6-phosphatase. although this strain after culturing at 30 degrees c was harmed by freezing, the cryotolerance of this strain was reached about 80% after cold acclimation at 10 degrees c. | 2002 | 12036064 |
| the autoregulatory role of esar, a quorum-sensing regulator in pantoea stewartii ssp. stewartii: evidence for a repressor function. | capsular polysaccharide synthesis and virulence in the plant pathogenic bacterium pantoea stewartii ssp. stewartii requires the quorum-sensing regulatory proteins, esar and esai, and the diffusible inducer n-(3-oxo-hexanoyl)-l-homoserine lactone. prior mutational studies suggested that esar might function as a repressor of quorum sensing in the control of capsular polysaccharide synthesis. further, a lux box-like palindromic sequence coinciding with the putative -10 element of the esar promoter ... | 2002 | 12067349 |
| plant-dependent genotypic and phenotypic diversity of antagonistic rhizobacteria isolated from different verticillium host plants. | to study the effect of plant species on the abundance and diversity of bacterial antagonists, the abundance, the phenotypic diversity, and the genotypic diversity of rhizobacteria isolated from potato, oilseed rape, and strawberry and from bulk soil which showed antagonistic activity towards the soilborne pathogen verticillium dahliae kleb. were analyzed. rhizosphere and soil samples were taken five times over two growing seasons in 1998 and 1999 from a randomized field trial. bacterial isolates ... | 2002 | 12089011 |
| fitness of salmonella enterica serovar thompson in the cilantro phyllosphere. | the epiphytic fitness of salmonella enterica was assessed on cilantro plants by using a strain of s. enterica serovar thompson that was linked to an outbreak resulting from cilantro. salmonella serovar thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning microscopy (clsm) at high densities on the veins and in natural lesions. the population sizes of two common colonizers of plant surfaces, pantoea agglomerans and pseudomonas chloro ... | 2002 | 12089050 |
| the effect of nitrogen and carbon sources on growth of the biocontrol agent pantoea agglomerans strain cpa-2. | the effect of several nitrogen and carbon sources on the growth of pantoea agglomerans (strain cpa-2) was studied for the first time. | 2002 | 12100585 |
| construction and characterization of a prou-gfp transcriptional fusion that measures water availability in a microbial habitat. | we constructed and characterized a transcriptional fusion that measures the availability of water to a bacterial cell. this fusion between the prou promoter from escherichia coli and the reporter gene gfp was introduced into strains of e. coli, pantoea agglomerans, and pseudomonas syringae. the prou-gfp fusion in these bacterial biosensor strains responded in a quantitative manner to water deprivation caused by the presence of nacl, na(2)so(4), kcl, or polyethylene glycol (molecular weight, 8000 ... | 2002 | 12200319 |
| temporal distribution of chaetocnema pulicaria (coleoptera: chrysomelidae) populations in iowa. | in 1999 and 2000, yellow sticky cards and sweep net samples were used to document the occurrence of an overwintering adult generation of chaetocnema pulicaria melsheimer, corn flea beetle, followed by two distinct populations peaks during the growing season in iowa emergence of the overwintering adult generation started in mid-april and continued until early june in both years, with populations as high as 45 +/- 7.9 per 10 sweeps. periods that ranged from 14 to 32 d were observed in 1999 and 200 ... | 2002 | 12216815 |
| differences in attachment of salmonella enterica serovars and escherichia coli o157:h7 to alfalfa sprouts. | numerous salmonella enterica and escherichia coli o157:h7 outbreaks have been associated with contaminated sprouts. we examined how s. enterica serovars, e. coli serotypes, and nonpathogenic bacteria isolated from alfalfa sprouts grow on and adhere to alfalfa sprouts. growth on and adherence to sprouts were not significantly different among different serovars of s. enterica, but all s. enterica serovars grew on and adhered to alfalfa sprouts significantly better than e. coli o157:h7. e. coli o15 ... | 2002 | 12324317 |
| diversity of endophytic bacterial populations and their interaction with xylella fastidiosa in citrus plants. | citrus variegated chlorosis (cvc) is caused by xylella fastidiosa, a phytopathogenic bacterium that can infect all citrus sinensis cultivars. the endophytic bacterial communities of healthy, resistant, and cvc-affected citrus plants were studied by using cultivation as well as cultivation-independent techniques. the endophytic communities were assessed in surface-disinfected citrus branches by plating and denaturing gradient gel electrophoresis (dgge). dominant isolates were characterized by fat ... | 2002 | 12324338 |
| induced systemic resistance of selected endophytic bacteria against meloidogyne incognita on tomato. | in previous work, the four endophytic bacteria pantoea agglomerans mk-29, cedeca davisae mk-30, enterobacter spp. mk-42 and pseudomonas putida mt-19 were shown to reduce meloidogyne incognita on tomato when applied as a seed treatment and/or soil drench. the objective of this work was to study these bacteria for their potential to induce systemic resistance against root knot nematodes on tomato. to guarantee spatial separation between inducing agent and pathogen a split-root system was chosen an ... | 2001 | 12425091 |
| tonb of escherichia coli activates fhua through interaction with the beta-barrel. | fhua is a multifunctional protein in the outer membrane of escherichia coli that actively transports fe(3+)-ferrichrome and the antibiotics albomycin and rifamycin cgp 4832, and serves as a receptor for the unrelated phages t5, t1, phi80 and uc-1, colicin m and microcin j25. the energy source for active transport is the proton-motive force of the cytoplasmic membrane, which is required for all fhua functions except infection by phage t5, and is thought to be mediated to the outer-membrane recept ... | 2002 | 12427941 |
| [tibial osteitis caused by pantoea agglomerans after open grade iiib tibial shaft fracture]. | bone infection is not an uncommon complication of open tibial shaft fractures. we report an unusual case of chronic tibial osteitis caused by pantoea agglomerans after an open grade iiib tibial shaft fracture. p. agglomerans, an enterobacteria, was identified on bone samples obtained nine months after the fracture. the infection probably resulted from the initial soil contamination and developed despite fixation and bone covering with a muscle flap performed during the same operative time and ad ... | 2002 | 12447133 |
| preservation of chitinolytic pantoae agglomerans in a viable form by cellular dried alginate-based carriers. | improved viability of gram-negative bacteria during freeze-dehydration, storage, and soil inoculation is of crucial importance to their efficient application. the chitinolytic pantoae (enterobacter) agglomerans strain ic1270, a potential biocontrol agent of soil-borne plant-pathogenic fungi, was used as a model organism to study the efficacy of freeze-dried alginate-based beads (macrocapsules) as possible carriers for immobilized gram-negative bacterial cells. these macrocapsules were produced b ... | 2002 | 12467443 |
| effect of leaf surface waxes on leaf colonization by pantoea agglomerans and clavibacter michiganensis. | to evaluate the influence of leaf cuticular waxes on bacterial colonization of leaves, bacterial colonization patterns were examined on four glossy maize (zea mays l.) mutants that were altered in their cuticular wax biosynthesis. mutant gl3 was indistinguishable from the wild-type maize in its ability to foster colonization by the two bacterial species, pantoea agglomerans and clavibacter michiganensis subsp. nebraskensis. in contrast, the other three mutants supported the development of popula ... | 2002 | 12481996 |
| erwinia aphidicola, a new species isolated from pea aphid, acyrthosiphon pisum. | five strains of gram-negative, oxidase-negative, facultatively anaerobic, fermentative, motile, rod-shaped bacterium with the general characteristics of the family enterobacteriaceae were isolated from the gut of multiple specimens of the pea aphid. all the strains caused aphid mortality when ingested by insects via a synthetic diet. the results of biochemical tests showed that these strains are most related to erwinia herbicola and pantoea agglomerans. according to dna-dna hybridization, the fi ... | 1997 | 12501306 |
| phylogenetical relationship based on groe genes among phenotypically related enterobacter, pantoea, klebsiella, serratia and erwinia species. | in an attempt to define the phylogenetical relationship among 17 phenotypically related species of genera enterobacter, pantoea, serratia, klebsiella and erwinia, we determined almost all of their groe operon sequences using the polymerase chain reaction direct sequencing method. the number of nucleotide substitutions per site was 0.12+/-0.030. the value was 3.6-fold higher than that of 16s rdna. as a result, we were successful in constructing molecular phylogenetic trees which had a finer resol ... | 1997 | 12501307 |
| characterization of five phyllosphere bacteria isolated from rosa rugosa leaves, and their phenotypic and metabolic properties. | five gram-negative bacteria, all of which were enterobacteriaceae, were isolated from the phyllosphere of green or senescing leaves of rosa rugosa, and their phenotypic and physiological characteristics were examined. partial 16s rdna sequences led to identification of these isolates as pantoea agglomerans, klebsiella terrigena, erwinia rhapontici, and two strains of rahnella aquatilis. interestingly, these phyllosphere bacteria had certain phenotypic and physiological convergences, while they s ... | 2002 | 12506991 |
| quantitative assessment of antigen-specific cd8+ t cells in the mouse: application to vaccine research. | an effective hiv vaccine will likely need to induce potent and broad-based immunity, including cd8+ t cell responses. hence, a quantitative assay to measure such responses in animal models will be important in the evaluation of candidate hiv vaccines. we show here that a single immunization with hiv dna vaccines, followed by challenge with recombinant vaccinia virus expressing the relevant hiv antigen, allows quantitative assessment of cd8+ t cell responses. these responses can be profound (>30% ... | 2003 | 12527230 |
| [use transposon and parde through in vivo cloning to promote the genetic stability of plasmid pcpp430]. | plasmid pcpp430 carrying the hrp gene cluster after transformated into 308r (pantoea agglomerans) can cause the hypersensitive response, and simultaneously induce the plant resistance to disease. it was genetically unstable in p. agglomerans. 0.8 kb parde region of the broad-host range plasmid rk2 is responsible for plasmid partition. it can mediate plasmid maitenance of many kinds in rhizobium meliloti, and also can promote the genetic stability of recombinant plasmid in the biocontrol bacteria ... | 2001 | 12552820 |
| differences between pseudomonas syringae pv. syringae b728a and pantoea agglomerans brt98 in epiphytic and endophytic colonization of leaves. | the leaf colonization strategies of two bacterial strains were investigated. the foliar pathogen pseudomonas syringae pv. syringae strain b728a and the nonpathogen pantoea agglomerans strain brt98 were marked with a green fluorescent protein, and surface (epiphytic) and subsurface (endophytic) sites of bean and maize leaves in the laboratory and the field were monitored to see if populations of these strains developed. the populations were monitored using both fluorescence microscopy and counts ... | 2003 | 12571050 |
| cloning and expression of the gene encoding catalytic subunit of thermostable glucose dehydrogenase from burkholderia cepacia in escherichia coli. | we have cloned a 1620-nucleotide gene encoding the catalytic subunit (alpha subunit) of a thermostable glucose dehydrogenase (gdh) from burkholderia cepacia. the fad binding motif was found in the n-terminal region of the alpha subunit. the deduced primary structure of the alpha subunit showed about 48% identity to the catalytic subunits of sorbitol dehydrogenase (sdh) from gluconobacter oxydans and 2-keto-d-gluconate dehydrogenases (2kgdh) from erwinia herbicola and pantoea citrea. the alpha su ... | 2003 | 12573242 |
| microbiology of choledochal bile in patients with choledocholithiasis admitted to a tertiary hospital. | the present study was designed to investigate the microbiology of choledochal bile of patients with cholangitis and choledocholithiasis. | 2003 | 12603536 |
| isolation of a pantoea dispersa-like strain fron a 71-year-old woman with acute myeloid leukemia and multiple myeloma. | | 2003 | 12608369 |
| water activity, temperature, and ph effects on growth of the biocontrol agent pantoea agglomerans cpa-2. | the growth response of the biocontrol agent pantoea agglomerans to changes in water activity (a(w)), temperature, and ph was determined in vitro in nutrient yeast extract-sucrose medium. the minimum temperature at which p. agglomerans was able to grow was 267-272 kelvins (-6 to -1 degrees c), and growth of p. agglomerans did not change at varying ph levels (4.5-8.6). the minimum a(w) for growth was 0.96 in media modified with glycerol and 0.95 in media modified with nacl or glucose. solute used ... | 2002 | 12619821 |
| respiration of 13c-labeled substrates added to soil in the field and subsequent 16s rrna gene analysis of 13c-labeled soil dna. | our goal was to develop a field soil biodegradation assay using (13)c-labeled compounds and identify the active microorganisms by analyzing 16s rrna genes in soil-derived (13)c-labeled dna. our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soil associated with sampling and laboratory incubation. the new field-based assay involved the release of (13)c-labeled compounds (glucose, phenol, caffeine, and naphthalene) to soil ... | 2003 | 12620850 |
| quorum-sensing-directed protein expression in serratia proteamaculans b5a. | n-acyl-l-homoserine-lactone-producing serratia species are frequently encountered in spoiling foods of vegetable and protein origin. the role of quorum sensing in the food spoiling properties of these bacteria is currently being investigated. a set of luxr luxi homologous genes encoding a putative quorum sensor was identified in the n-(3-oxo-hexanoyl)-l-homoserine lactone (3-oxo-c6-hsl)-producing serratia proteamaculans strain b5a. the 3-oxo-c6-hsl synthase spri showed 79 % similarity with esai ... | 2003 | 12624209 |
| gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in arabidopsis. | the major regulatory shoot signal is auxin, whose synthesis in young leaves has been a mystery. to test the leaf-venation hypothesis [r. aloni (2001) j plant growth regul 20: 22-34], the patterns of free-auxin production, movement and accumulation in developing leaf primordia of dr5::gus-transformed arabidopsis thaliana (l.) heynh. were visualized. dr5::gus expression was regarded to reflect sites of free auxin, while immunolocalization with specific monoclonal antibodies indicated total auxin d ... | 2003 | 12624772 |
| mathematical modeling of in vitro enzymatic production of 2-keto-l-gulonic acid using nad(h) or nadp(h) as cofactors. | a 2-keto-l-gulonic acid (2-klg) production process using stationary pantoea citrea cells and a corynebacterium 2,5-diketo-d-gluconic acid (2,5-dkg) reductase enzyme has been developed which may represent an improved method of vitamin c biosynthesis. experimental data was collected using the f22y/a272g 2,5-dkg reductase mutant and nadp(h) as a cofactor. an extensive kinetic analysis was performed and a kinetic rate equation model for this process was developed. a recent protein engineering effort ... | 2002 | 12646322 |
| the hrpx/hrpy two-component system activates hrps expression, the first step in the regulatory cascade controlling the hrp regulon in pantoea stewartii subsp. stewartii. | a regulatory cascade activating hrp/hrc type iii secretion and effector genes was delineated in pantoea stewartii subsp. stewartii, a bacterial pathogen of corn. four hrp regulatory genes were characterized: hrpx and hrpy encode the sensor kinase and response regulator, respectively, of a two-component signal transduction system; hrps encodes an ntrc-like transcriptional enhancer; and hrpl encodes an alternative sigma factor. epistasis analysis, expression studies using gene fusions, and genetic ... | 2003 | 12650455 |
| bacteriophages of erwinia amylovora. | fifty bacteriophage isolates of erwinia amylovora, the causal agent of fire blight, were collected from sites in and around the niagara region of southern ontario and the royal botanical gardens, hamilton, ontario. forty-two phages survived the isolation, purification, and storage processes. the majority of the phages in the collection were isolated from the soil surrounding trees exhibiting fire blight symptoms. only five phages were isolated from infected aerial tissue in pear and apple orchar ... | 2003 | 12676693 |
| bacteriocin serratine-p as a biological tool in the control of fire blight erwinia amylovora. | fire blight, caused by the bacterium erwinia amylovora (burill winslow et al.), is the most important bacterial disease in european pear growing. it can cause a lot of damage in some countries on apple and on pear trees in orchards and also in the fruit tree nurseries. in belgium, the disease is present since 1972. control of fire blight in belgian fruit orchards is made on a broad basis of measurements in and around the fruit trees. the use of an antibiotic is allowed for application only durin ... | 2002 | 12701444 |
| quorum sensing in plant-pathogenic bacteria. | quorum sensing (qs) allows bacteria to assess their local population density and/or physical confinement via the secretion and detection of small, diffusible signal molecules. this review describes how phytopathogenic bacteria have incorporated qs mechanisms into complex regulatory cascades that control genes for pathogenicity and colonization of host surfaces. traits regulated by qs include the production of extracellular polysaccharides, degradative enzymes, antibiotics, siderophores, and pigm ... | 2003 | 12730390 |
| structural analysis of the dna-binding domain of the erwinia amylovora rcsb protein and its interaction with the rcsab box. | the transcriptional regulator rcsb interacts with other coactivators to control the expression of biosynthetic operons in enterobacteria. while in a heterodimer complex with the regulator rcsa the rcsab box consensus is recognized, dna binding sites for rcsb without rcsa have also been identified. the conformation of rcsb might therefore be modulated upon interaction with various coactivators, resulting in the recognition of different dna targets. we report the solution structure of the c-termin ... | 2003 | 12740396 |
| pantoea agglomerans as a cause of septic arthritis after palm tree thorn injury; case report and literature review. | we report the case of a 14 year old healthy boy, who was admitted six weeks after being injured by a palm tree thorn, with limping caused by pain and swelling in his right knee. an ultrasound examination revealed a foreign body in the posterior lateral aspect of the right knee. pantoea agglomerans was identified in the synovial fluid. the patient underwent two arthrotomies and was treated with amoxicillin-clavulanate intravenously for three weeks. the postoperative course was uneventful, and joi ... | 2003 | 12765929 |
| biological control of monilinia laxa and rhizopus stolonifer in postharvest of stone fruit by pantoea agglomerans eps125 and putative mechanisms of antagonism. | treatment of stone fruits (apricot, peach and nectarine) with pantoea agglomerans strain eps125 decreased the incidence and diameter of lesions of brown rot caused by monilinia laxa and soft rot caused by rhizopus stolonifer. root control was achieved on fruits either wounded and subsequently inoculated with the pathogens or non-wounded and naturally infected from orchards. the efficacy of biocontrol was dependent on the concentration of the biocontrol agent and pathogen. at medium to low pathog ... | 2003 | 12781959 |
| structural and functional analysis of pantocin a: an antibiotic from pantoea agglomerans discovered by heterologous expression of cloned genes. | | 2003 | 12833353 |
| the biosynthetic gene cluster of pantocin a provides insights into biosynthesis and a tool for screening. | | 2003 | 12833354 |
| exposure to airborne gram-negative bacteria, dust and endotoxin in paper factories. | air samples for determination of the concentration of gram-negative bacteria, dust and endotoxin were collected at 10 sites in 2 large pulp and paper mills (paper factories) located in northern poland, of which one (plant "a") was an older type facility while the other (plant "b") was a modern, fully automated factory with an effective ventilatory system. in both factories paper was produced from wood chips derived mostly from scots pine. the concentrations of gram-negative bacteria in the air o ... | 2003 | 12852739 |
| structure of dried cellular alginate matrix containing fillers provides extra protection for microorganisms against uvc radiation. | soil microorganisms in general and biocontrol agents in particular are very sensitive to uv light. the packaging of biocontrol microorganisms into cellular solids has been developed as a means of reducing loss caused by exposure to environmental uv radiation. the bacterial and fungal biocontrol agents pantoea agglomerans and trichoderma harzianum were immobilized in freeze-dried alginate beads containing fillers and subjected to 254 nm uv radiation (uvc). immobilization of cells in freeze-dried ... | 2003 | 12859230 |
| optical properties of erwinia herbicola bacteria at 0.190-2.50 microm. | we measure the complex index of refraction of erwina herbicola (also known as enterobacter agglomerans or pantoea agglomerans) bacteria (attc 33243) over the spectral region from 0.190 to 2.50 microm (4000-52,632 cm(-1)). transmission measurements are made on solid films of e. herbicola and on suspensions of the bacteria in water. these measurements, combined with spectral reflectance and kramers-krönig analysis, allow the determination of the real and imaginary parts over the entire wavelength ... | 2003 | 12949829 |
| the influence of antibiotic production and pre-emptive colonization on the population dynamics of pantoea agglomerans (erwinia herbicola) eh1087 and erwinia amylovora in planta. | stigma colonization by erwinia amylovora is the crucial first step in the development of most fire blight infections in apple and pear trees. suppression at this point of the disease process by antagonists of e. amylovora, such as pantoea agglomerans (erwinia herbicola) strain eh1087, is a rational approach to control fire blight. we tested the hypothesis that the ability of e. amylovora to compete with eh1087 for colonization of a stigma is reduced by the potential for eh1087 to produce the phe ... | 2003 | 14510856 |
| properties of a novel extracellular cell-free ice nuclei from ice-nucleating pseudomonas antarctica in-74. | some ice-nucleating bacterial strains, including pantoea ananatis (erwinia uredovora), pseudomonas fluorescens, and pseudomonas syringae isolates, were examined for the ability to shed ice nuclei into the growth medium. a novel ice-nucleating bacterium, pseudomonas antarctica in-74, was isolated from ross island, antarctica. cell-free ice nuclei from p. antarctica in-74 were different from the conventional cell-free ice nuclei and showed a unique characterization. cell-free ice nuclei were purif ... | 2003 | 14519981 |
| effects of gypsophila saponins on bacterial growth kinetics and on selection of subterranean clover rhizosphere bacteria. | plant secondary metabolites, such as saponins, have a considerable impact in agriculture because of their allelopathic effects. they also affect the growth of soil microorganisms, especially fungi. we investigated the influence of saponins on rhizosphere bacteria in vitro and in soil conditions. the effects of gypsophila saponins on the growth kinetics of rhizosphere bacteria were studied by monitoring the absorbance of the cultures in microtiter plates. gypsophila saponins (1%) increased the la ... | 2003 | 14569290 |
| recovery of surface bacteria from and surface sanitization of cantaloupes. | practical, effective methods that could be implemented in a food service establishment (restaurant or delicatessen) for the surface sanitization of cantaloupes were microbiologically evaluated. cantaloupes (cucumis melo l. var. reticulates) were immersed in an inoculum containing salmonella enterica serovar poona or pantoea agglomerans at ca. 10(4) to 10(5) cfu/ml. an efficient method for the recovery of bacteria from the cantaloupe surface was developed and validated. the method consisted of wa ... | 2003 | 14572217 |
| the quorum sensing negative regulators esar and expr(ecc), homologues within the luxr family, retain the ability to function as activators of transcription. | most luxr homologues function as activators of transcription during the process of quorum sensing, but a few, including esar and expr(ecc), negatively impact gene expression. the luxr-activated luxi promoter and luxr binding site, the lux box, were used in artificial contexts to assess the potential for transcriptional activation and dna binding by esar and expr(ecc). although the acyl-homoserine lactone responsiveness of both proteins is the opposite of that shown by most luxr family members, e ... | 2003 | 14617666 |
| diverse microbial communities inhabiting ferromanganese deposits in lechuguilla and spider caves. | lechuguilla cave is an ancient, deep, oligotrophic subterranean environment that contains an abundance of low-density ferromanganese deposits, the origin of which is uncertain. to assess the possibility that biotic factors may be involved in the production of these deposits and to investigate the nature of the microbial community in these materials, we carried out culture-independent, small subunit ribosomal rna (ssu rrna) sequence-based studies from two sites and from manganese and iron enrichm ... | 2003 | 14641587 |
| isolation of endophytic diazotroph pantoea agglomerans and nondiazotroph enterobacter asburiae from sweetpotato stem in japan. | to isolate and identify diazotrophic endophytes in the stem of japanese sweetpotato cv. koganesengan. | 2004 | 14687210 |
| the periplasmic serine protease inhibitor ecotin protects bacteria against neutrophil elastase. | ecotin is a dimeric periplasmic protein from escherichia coli that has been shown to inhibit potently many trypsin-fold serine proteases of widely varying substrate specificity. to help elucidate the physiological function of ecotin, we examined the family of ecotin orthologues, which are present in a subset of gram-negative bacteria. phylogenetic analysis suggested that ecotin has an exogenous target, possibly neutrophil elastase. recombinant protein was expressed and purified from e. coli, yer ... | 2004 | 14705961 |
| asymmetrically acting lycopene beta-cyclases (crtlm) from non-photosynthetic bacteria. | carotenoids have important functions in photosynthesis, nutrition, and protection against oxidative damage. some natural carotenoids are asymmetrical molecules that are difficult to produce chemically. biological production of carotenoids using specific enzymes is a potential alternative to extraction from natural sources. here we report the isolation of lycopene beta-cyclases that selectively cyclize only one end of lycopene or neurosporene. the crtlm genes encoding the asymmetrically acting ly ... | 2004 | 14740205 |
| norm, an erwinia amylovora multidrug efflux pump involved in in vitro competition with other epiphytic bacteria. | blossoms are important sites of infection for erwinia amylovora, the causal agent of fire blight of rosaceous plants. before entering the tissue, the pathogen colonizes the stigmatic surface and has to compete for space and nutrient resources within the epiphytic community. several epiphytes are capable of synthesizing antibiotics with which they antagonize phytopathogenic bacteria. here, we report that a multidrug efflux transporter, designated norm, of e. amylovora confers tolerance to the tox ... | 2004 | 14766544 |
| trap height and orientation of yellow sticky traps affect capture of chaetocnema pulicaria (coleoptera: chrysomelidae). | field studies were conducted in iowa during 2001 and 2002 to determine the optimal sampling height and orientation for using yellow sticky cards to monitor populations of chaetocnema pulicaria melsheimer, the vector of the bacterial pathogen pantoea stewartii subsp, stewartii, the causal organism of stewart's disease of corn, zea mays l.. sticky cards were placed at five different heights (0.15, 0.30, 0.45, 0.60, and 0.90 m) and three orientations (horizontal, vertical, and 30 degree angle) at t ... | 2004 | 14998138 |
| [investigation of the bacteria in the seawater of xisha in the south china sea and their antibiotic sensitivity profile]. | to investigate the distribution and antibiotic sensitivity of the bacteria in the seawater of xisha in the south china sea. | 2004 | 15041561 |
| production of n-acylhomoserine lactone signal molecules by gram-negative soil-borne and plant-associated bacteria. | quorum-sensing control mediated by n-acylhomoserine lactone (ahl) signal molecules has been established as a key feature in the regulation of various metabolic traits in many bacteria. approximately 300 strains representing 6 genera and 18 species of soil-borne and plant-associated gram-negative bacteria isolated in various regions of the former ussr using two reporter systems were screened for ahl production. the production was observed in 17.5% of the screened bacterial strains. positive respo ... | 2003 | 15058194 |
| combining pantoea agglomerans (cpa-2) and curing treatments to control established infections of penicillium digitatum on lemons. | the effectiveness of the strain cpa-2 of pantoea agglomerans alone or in combination with a curing treatment at 33 degrees c for 65 h to control green mold was evaluated on lemons stored at ambient temperature and in cold storage. an application of p. agglomerans at 2 x 10(8) cfu/ml effectively reduced green mold incidence on recently inoculated lemons stored at temperatures from 5 to 25 degrees c. moreover, a 30-s immersion of lemons in a p. agglomerans suspension at 2 x 10(8) cfu/ml significan ... | 2004 | 15083731 |
| endophytic colonization ability of two deep-water rice endophytes, pantoea sp. and ochrobactrum sp. using green fluorescent protein reporter. | colonization ability of the two endophytic bacteria, isolated from surface sterilized seeds of jaisurya variety of deep-water rice viz., pantoea sp. and ochrobactrum sp., was compared after genetically tagging them with a constitutively expressing green fluorescent protein gene (gfp). confocal laser scanning microscopy (clsm) of hydroponically grown seedlings of jaisurya rice, inoculated with gfp-tagged endophytes, revealed that both pantoea sp. and ochrobactrum sp. colonized the intercellular s ... | 2004 | 15104142 |
| modeling the rate of attachment of listeria monocytogenes, pantoea agglomerans, and pseudomonas fluorescens to, and the probability of their detachment from, potato tissue at 10 degrees c. | the rate of attachment of bacteria to, and their subsequent detachment from, the cut surface of raw potato tissue was measured and modeled by using mathematical approaches that allowed detailed objective comparisons of adhesion processes under different conditions. attachment was rapid and reached equilibrium after contact for 60 min. a new method to measure the probability of detachment was developed and modeled, revealing that the probability of detachment for pseudomonas fluorescens remained ... | 2004 | 15184157 |
| characterization of pantoea dispersa uq68j: producer of a highly efficient sucrose isomerase for isomaltulose biosynthesis. | isolation, identification and characterization of a highly efficient isomaltulose producer. | 2004 | 15186446 |
| a family of conserved bacterial effectors inhibits salicylic acid-mediated basal immunity and promotes disease necrosis in plants. | salicylic acid (sa)-mediated host immunity plays a central role in combating microbial pathogens in plants. inactivation of sa-mediated immunity, therefore, would be a critical step in the evolution of a successful plant pathogen. it is known that mutations in conserved effector loci (cel) in the plant pathogens pseudomonas syringae (the delta cel mutation), erwinia amylovora (the dspa/e mutation), and pantoea stewartii subsp. stewartii (the wtse mutation) exert particularly strong negative effe ... | 2004 | 15210989 |
| studies on cultured and uncultured microbiota of wild culex quinquefasciatus mosquito midgut based on 16s ribosomal rna gene analysis. | to describe the midgut microbial diversity and the candidate bacteria for the genetic manipulation for the generation of transgenic mosquitoes refractory to transmission of diseases, the microbiota of wild culex quinquefasciatus mosquito midgut was studied using a conventional culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. the culturable microbiota was identified as acinetobacter junii, ac. calcoaceticus, aeromonas culicicola, bacillus thuringiensis, microbac ... | 2004 | 15210998 |
| purification and characterization of uridine phosphorylase from the ice-nucleating bacterium, pantoea agglomerans nbrc12686. | the ice-nucleating bacterium, pantoea agglomerans nbrc12686 responds to a decrease in temperature with the induction of proteins, which are classified as cold-induced proteins. when the temperature of the strain nbrc12686 culture was lowered from 30 degree c to 12 degree c, the viability after freezing treatment significantly improved. by the use of sds-polyacrylamide gel electrophoresis and high-performance liquid chromatography (hplc), we analyzed the cold acclimation response in strain nbrc12 ... | 2004 | 15216384 |
| immunologic reactivity to work-related airborne allergens in people occupationally exposed to dust from herbs. | a group of 150 people occupationally exposed to dust from herbs were examined. the examined group consisted of 47 thyme farmers, 32 chamomile farmers, 31 sage farmers and 40 workers of herbs processing industry. as a reference group, 50 urban dwellers, not exposed to any kind of organic dust, were examined. skin prick tests and precipitin tests were conducted with, respectively, 4 and 11 microbial antigens associated with organic dust. both skin and precipitin tests were also conducted with herb ... | 2004 | 15236509 |
| pantoea agglomerans and thorn-associated suppurative arthritis. | | 2004 | 15247618 |
| a putative new endophytic nitrogen-fixing bacterium pantoea sp. from sugarcane. | to isolate and identify endophytic nitrogen-fixing bacteria in sugarcane growing in cuba without chemical fertilizers. | 2004 | 15281930 |
| health effects of inhalation exposure to organic dust in hops farmers. | medical examinations were performed in a group of 23 hops farmers exposed to organic dust from hop (humulus lupulus). the examinations took place in individual farms during harvesting, sorting and transporting of hop cones. as a reference group, 50 urban dwellers not exposed to organic dust were examined. there were conducted physical examinations, interviews concerning the occurrence of respiratory disorders and work-related symptoms, lung function tests, determination of cytokines concentratio ... | 2003 | 15315032 |
| isolation and identification of marine chitinolytic bacteria and their potential in antifungal biocontrol. | chitinolytic marine bacterial strains (30) were isolated from the sea dumps at bhavnagar, india. they were screened as chitinase producers on the basis of zone of clearance on chitin agar plates incorporated with calcofluor white m2r for the better resolution. out of these, three strains namely, pseudomonas sp., pantoea dispersa and enterobacter amnigenus showed high chitinase production. they were also found to produce proteases and therefore have a good potential for use as antifungal biocontr ... | 2004 | 15339036 |
| malt sprout extract medium for cultivation of lactobacillus plantarum protective cultures. | the aim was to develop a cheap cereal-based alternative medium for the large-scale production of biopreservative lactobacillus plantarum vtt e-79098. we examined the effect of growth medium and ph control on the cell yield of lact. plantarum e-79098 and the antimicrobial activity of the cell-free extracts. | 2004 | 15355535 |
| vibrio spp. and salmonella spp., presence and susceptibility in crabs ucides cordatus. | the presence of vibrio spp. and salmonella spp. in crabs marketed at the bezerra de menezes ave., fortaleza, state of ceará, brazil, was assessed between february and may, 2003. the number of individuals sampled in each one of the fifteen weekly samplings ranged between four and eight. seven strains of salmonella, from four different samplings, were identified, being five of them identified as serotype s. senftenberg and two as s. poona. all strains of salmonella were sensitive to the tested ant ... | 2004 | 15361967 |
| a selective differential medium for enterobacter sakazakii, a preliminary study. | enterobacter sakazakii can cause fatal invasive infection of neonates associated with the presence of this organism in powdered infant milk formula. a new chromogenic medium (druggan-forsythe-iversen agar, dfi) is described for the selective detection of this emergent pathogen. the medium is based on the alpha-glucosidase reaction which is detected using 5-bromo-4-chloro-3-indolyl-alpha,d-glucopyranoside (xalphaglc). ent. sakazakii hydrolyses this substrate to an indigo pigment, producing blue-g ... | 2004 | 15364468 |
| bacteremic infection with pantoea ananatis. | a 73-year-old man was hospitalized for dyspnea and bilateral ankle edema. during his hospital stay he presented anal hemorrhage and developed a high fever after colonoscopy. a set of aerobic and anaerobic blood culture bottles yielded a pure culture of gram-negative rods, susceptible to all antibiotics tested. the api20e code was 1005133, resulting in a very good identification as pantoea sp. subsequent sequencing of the 16s rrna gene revealed a final identification as pantoea ananatis. the pati ... | 2004 | 15365053 |
| gamma proteobacteria can nodulate legumes of the genus hedysarum. | the bacteria hosted in the root nodules of the three mediterranean wild legume species hedysarum carnosum, hedysarum spinosissimum subsp. capitatum, and hedysarum pallidum, growing in native stands in different habitats in algeria were isolated. bacteria were recovered on yeast-mannitol-agar or on minimal media from a total of 52 nodules. isolates were analyzed by amplified ribosomal dna restriction analysis (ardra) using the enzyme cfoi, and further sorted by rapd fingerprinting. a total of ten ... | 2004 | 15368852 |
| proposal to acknowledge beijerinck as the original author of the species pantoea agglomerans. request for an opinion. | the name 'bacillus agglomerans' was first published by beijerinck in 1888. ewing and fife changed the name to enterobacter agglomerans in 1972 as a new combination, acknowledging that beijerinck had been the original author and making the new full name enterobacter agglomerans (beijerinck 1888) ewing and fife 1972. beijerinck's name was omitted from the approved lists of bacterial names in 1980, which listed only ewing and fife as the authors. the current listings in the list of bacterial names ... | 2004 | 15545493 |
| identification of enterobacteriaceae from washed and unwashed commercial shell eggs. | to evaluate the effect of processing on the safety and quality of retail shell eggs, a storage study was conducted with unwashed and commercially washed eggs. this work demonstrated that commercial processing decreased microbial contamination of eggshells. to know which species persisted during storage on washed or unwashed eggs, enterobacteriaceae isolates were selected and identified biochemically. for each of three replications, shell eggs were purchased from a commercial processing plant, tr ... | 2004 | 15553650 |
| isolation and characterization of soybean-associated bacteria and their potential for plant growth promotion. | endophytic and epiphytic bacteria were isolated from two soybean cultivars (foscarin and cristalina). significant differences were observed in bacterial population densities in relation to season of isolation, soybean growth phase and the tissues from which the isolates were obtained. the isolates were identified by partial 16s rdna sequence analysis, with most of the isolates belonging to the pseudomonaceae, burkholderiacea and enterobacteriaceae groups. the potential of the isolates for plant ... | 2004 | 15560822 |
| formulation of medium constituents by multiresponse analysis of central composite design to enhance chitinase production in pantoea dispersa. | in the present study, a high chitinase producing strain pantoea dispersa was isolated from the sea dumps at bhavnagar, india. chitin, urea, cacl2 and mgso4 x 7h2o were variables used in central composite design for chitinase production. chitinase, biomass and ph were the responses used in different models to evaluate individually fit ones. quadratic model was found to be fit for chitinase response whereas in the case of biomass and ph, linear model was found to be fit without the effect of other ... | 2004 | 15587121 |
| exposure to airborne microorganisms, dust and endotoxin during flax scutching on farms. | microbiological air sampling was performed on 5 flax farms located in eastern poland. air samples for determination of the concentrations of microorganisms, dust and endotoxin were collected in barns during machine scutching of flax stems by the farmers. the concentrations of mesophilic bacteria ranged from 203.5-698.8 x 10(3) cfu/m3, of gram-negative bacteria from 27.2-123.4 x 10(3) cfu/m3, of thermophilic actinomycetes from 0.5-2.6 x 10(3) cfu/m3, and of fungi from 23.4-99.8 x 10(3) cfu/m3. th ... | 2004 | 15627342 |
| studies on the occurrence of gram-negative bacteria in ticks: ixodes ricinus as a potential vector of pasteurella. | a total of 372 ixodes ricinus ticks (101 females, 122 males, and 149 nymphs) collected by flagging in 6 mixed woodlands of eastern poland were examined by culture for the presence of internal gram-negative bacteria other than borrelia burgdorferi. adult ticks were examined in pools of 2 specimens each and nymphs were examined in pools of 3-5 specimens each. ticks were disinfected in 70 % ethanol and homogenized in 0.85% nacl. the diluted homogenate was inoculated onto 3 kinds of agar media: buff ... | 2004 | 15627343 |
| a farmer's occupational airborne contact dermatitis masqueraded by coexisting rosacea: delayed diagnosis and legal acknowledgement. | a rare case of coexistence of occupational airborne dermatitis with rosacea is presented in a 41-year-old female farmer. her first dermatitis symptoms appeared at the age of 10 when she started helping her parents on the farm. uncovered skin areas of the face, neck, décolleté, forearms and the hands gradually became involved. the dermatitis symptoms were provoked by agricultural dusts (especially of flax and dried herbs). for the subsequent 30 years, the work-related disease remained undiagnosed ... | 2004 | 15627345 |
| a predictive model for heat inactivation of listeria monocytogenes biofilm on stainless steel. | heat treatment of potential biofilm-forming sites is sometimes used for control of listeria monocytogenes in food processing plants. however, little information is available on the heat treatment required to kill l. monocytogenes present in biofilms. the purpose of this study was to develop a predictive model for the heat inactivation of l. monocytogenes in monoculture biofilms (strains scott a and 3990) and in biofilms with competing bacteria (pseudomonas sp. and pantoea agglomerans) formed on ... | 2004 | 15633676 |
| performance comparison of the biosys optical assay and the violet red bile agar method for detecting coliforms in food products. | coliform counts in a variety of foods, including dairy products (raw milk, pasteurized milk, yogurt, butter, and ice cream), meats (pork sausage, ground beef, and raw chicken), raw eggs, and chocolate, were performed by the rapid automated biosys optical assay and the conventional method with violet red bile agar (vrba). the standard deviation (sd) among five replicate counts for the optical assay was similar to or better than that obtained with vrba plates for all foods tested. the average sd f ... | 2004 | 15633683 |
| altering substrate chain length specificity of an acylhomoserine lactone synthase in bacterial communication. | quorum sensing mediated by specific signal compounds (autoinducers) allows bacteria to monitor their cell density and enables a synchronized regulation of target gene sets. the best studied group of autoinducers are the acylhomoserine lactones (ahsls), which are central to the regulation of virulence in many plant and animal pathogens. variation of the acyl side chain of the ahsls underlies the observed species specificity of this communication system. here we show that even different strains of ... | 2005 | 15634689 |
| assessment of bacterial contamination of drinking water provided to mice. | the objective of this study was to evaluate whether an 240-ml water bottle provided to individually housed mice would remain potable for a 2-week interval (based on absence of coliforms). the study used inbred c57bl/6 mice and cb6f1 x c3d2f1 hybrid mice. test groups were assigned to minimize the variables of strain, caging type (non-ventilated static versus ventilated) and building location. a 3-cc sample of drinking water was removed aseptically from the bottles and vacuum-filtered using a 250- ... | 2004 | 15636548 |
| role of glutamine synthetase in phenazine antibiotic production by pantoea agglomerans eh1087. | pantoea agglomerans strain eh1087 produces the phenazine antibiotic d-alanylgriseoluteic acid. a glutamine auxotroph harboring an insertion in a putative glna gene was obtained by transposon-mutagenesis of eh1087 that produced less d-alanylgriseoluteic acid than the parental strain (strain eh7.1). cosmids encoding the eh1087 glna were isolated by their ability to complement the mutant for prototrophy. the role of the eh1087 glna locus was functionally confirmed by complementation of an escherich ... | 2004 | 15644904 |
| purification and properties of a phytate-degrading enzyme from pantoea agglomerans. | a periplasmatic phytate-degrading enzyme from pantoea agglomerans isolated from soil was purified about 470-fold to apparent homogeneity with a recovery of 16% referred to the phytate-degrading activity in the crude extract. it behaved as a monomeric protein with a molecular mass of about 42 kda. the purified enzyme exhibited a single ph optimum at 4.5. optimum temperature for the degradation of phytate was 60 degrees c. the kinetic parameters for the hydrolysis of sodium phytate were determined ... | 2004 | 15648978 |
| degradation of myo-inositol hexakisphosphate by a phytate-degrading enzyme from pantoea agglomerans. | high-pressure liquid chromatography (hplc) analysis established myo-inositol pentakisphosphate as the final product of phytate dephosphorylation by the phytate-degrading enzyme from pantoea agglomerans. neither product inhibition by phosphate nor inactivation of the pantoea enzyme during the incubation period were responsible for the limited phytate hydrolysis as shown by addition of phytate-degrading enzyme and phytate, respectively, after the observed stop of enzymatic phytate degradation. in ... | 2004 | 15648979 |