| morphological features of regurgitate and defecatory stains deposited by five species of necrophagous flies are influenced by adult diets and body size. | the morphological characteristics of artifacts from five species of necrophagous flies were examined following feeding on several types of diets. four types of insect stains were produced by each species: regurgitate, defecatory, translocation, and tarsal tracks. regurgitate was the most frequent type deposited (70.9 ± 2.4%), followed by defecatory (19.8 ± 4.0%), tarsal tracks (8.6 ± 1.2%), and translocation (0.7 ± 0.1%). artifact shapes, sizes, and color were highly variable and species and die ... | 2018 | 28230912 |
| distribution modeling of three screwworm species in the ecologically diverse landscape of north west pakistan. | north west pakistan (nwp) is characterized by four eco-zones: northern montane region, north western hills, submontane region and indus plains. present study identified 1037 cases of traumatic myiasis in the region during 2012-2015. screw worm larvae were classified as 12 species: chrysomya bezziana (villeneuve), chryomya megacephala (fabricius), chrysomya rufifacies (macquart), lucilia cuprina (wiedemann), lucilia sericata (meigen), lucilia illustris (meigen), lucilia porphyrina (walker), hemip ... | 2016 | 27311386 |
| development and validation of a new technique for estimating a minimum postmortem interval using adult blow fly (diptera: calliphoridae) carcass attendance. | understanding the onset and duration of adult blow fly activity is critical to accurately estimating the period of insect activity or minimum postmortem interval (minpmi). few, if any, reliable techniques have been developed and consequently validated for using adult fly activity to determine a minpmi. in this study, adult blow flies (diptera: calliphoridae) of cochliomyia macellaria and chrysomya rufifacies were collected from swine carcasses in rural central texas, usa, during summer 2008 and ... | 2015 | 25367772 |
| effect of massing on larval growth rate. | estimation of minimum postmortem interval commonly relies on predicting the age of blowfly larvae based on their size and an estimate of the temperatures to which they have been exposed throughout their development. the majority of larval growth rate data have been developed using small larval masses in order to avoid excess heat generation. the current study collected growth rate data for larvae at different mass volumes, and assessed the temperature production of these masses, for two forensic ... | 2014 | 24972255 |
| tracking movement and temperature selection of larvae of two forensically important blow fly species within a "maggot mass". | the current study responds to the lack of understanding about the temperatures experienced by individual blow fly larvae within "maggot masses." the temperature selection of both aggregating (in a mass) and nonaggregating larvae was compared and their pattern of movement assessed. infrared imaging determined the temperatures within a mass and in the vicinity of the constituent individual larvae, whose movements were tracked by dyeing their tissues red. individual chrysomya rufifacies larvae sele ... | 2014 | 24621385 |
| ultrastructure of male genitalia of blow flies (diptera: calliphoridae) of forensic importance. | male genitalia of blow flies (diptera: calliphoridae) are distinctive in their morphological features and are often used for species identification. the aim of this work was to investigate the male genitalia of blow flies of medical and forensic importance from thailand at the ultrastructural level, using scanning electron microscopy (sem). flies in two subfamilies were examined: chrysomyinae [chrysomya bezziana villeneuve, chrysomya chani kurahashi, chrysomya nigripes aubertin, chrysomya pingui ... | 2017 | 29248415 |
| variation in the time of colonization of broiler carcasses by carrion flies in nakhonsawan province, thailand. | carrion flies are the primary insects colonizing vertebrate carrion; however, limited information is available on the variation in the time of colonization (toc) as related to time of placement (top) and time of death (tod), particularly in thailand. three seasonal sets of nine broiler carcasses (euthanized and placed in field within 0.5 h after death) were placed in mesh enclosures within a disturbed deciduous dipterocarp forest at nakhonsawan province, upper-central thailand, for 3 d to determ ... | 2017 | 28444209 |
| nonconsumptive effects of predatory chrysomya rufifacies (diptera: calliphoridae) larval cues on larval cochliomyia macellaria (diptera: calliphoridae) growth and development. | forensic entomologists often rely on development data associated with a given species to estimate when it colonized human or other vertebrate remains. in most instances, these development studies are based on single species reared in isolation in the laboratory. this study examined the impact of excretions and secretions (es) associated with third-instar chrysomya rufifacies (macquart), a predator, on the development of its prey, cochliomyia macellaria (f.). not surprisingly, ch. rufifacies es d ... | 2017 | 28541462 |
| predictable weathering of puparial hydrocarbons of necrophagous flies for determining the postmortem interval: a field experiment using chrysomya rufifacies. | preadult development of necrophagous flies is commonly recognized as an accurate method for estimating the minimum postmortem interval (pmimin). however, once the pmimin exceeds the duration of preadult development, the method is less accurate. recently, fly puparial hydrocarbons were found to significantly change with weathering time in the field, indicating their potential use for pmimin estimates. however, additional studies are required to demonstrate how the weathering varies among species. ... | 2017 | 28058571 |
| fluorescence imaging of posterior spiracles from second and third instars of forensically important chrysomya rufifacies (diptera: calliphoridae). | entomological protocols for aging blowfly (diptera: calliphoridae) larvae to estimate the time of colonization (toc) are commonly used to assist in death investigations. while the methodologies for analyzing fly larvae differ, most rely on light microscopy, genetic analysis, or, more rarely, electron microscopy. this pilot study sought to improve resolution of larval stage in the forensically important blowfly chrysomya rufifacies using high-content fluorescence microscopy and biochemical measur ... | 2016 | 27706817 |
| larval development rates of chrysomya rufifacies macquart, 1842 (diptera: calliphoridae) within its native range in south-east asia. | chrysomya rufifacies represents an important indicator species in forensic entomology that is often used to estimate the minimum postmortem interval (pmimin) in crime scene investigation. however, developmental rates differ locally, so that estimates should be based on regionally generated development data. therefore, we determined the developmental rates of c. rufifacies within its native range in thailand under nine constant temperature regimes: 15, 18, 21, 24, 27, 30, 33, 36 and 39°c. develop ... | 2016 | 27213919 |
| development of the oriental latrine fly, chrysomya megacephala (diptera: calliphoridae), at five constant temperatures. | chrysomya megacephala (fabricius) is a forensically important fly that is found throughout the tropics and subtropics. we calculated the accumulated development time and transition points for each life stage from eclosion to adult emergence at five constant temperatures: 15, 20, 25, 30, and 35 °c. for each transition, the 10th, 50th, and 90th percentiles were calculated with a logistic linear model. the mean transition times and % survivorship were determined directly from the raw laboratory dat ... | 2017 | 27816915 |
| study on the pupal morphogenesis of chrysomya rufifacies (macquart) (diptera: calliphoridae) for postmortem interval estimation. | chrysomya rufifacies (macquart) is one of the most common species of blow flies at the scene of death in southern china. pupae are useful in postmortem interval (pmi) estimation due to their sedentary nature and longer duration of association with the corpse. however, to determine the age of a pupa is more difficult than that of a larva, due to the fact that morphological changes are rarely visible during pupal development. in this study, eggs of c. rufifacies were reared in climatic chambers un ... | 2015 | 26112814 |
| use of dna sequences to identify forensically important fly species and their distribution in the coastal region of central california. | forensic entomology has gained prominence in recent years, as improvements in dna technology and molecular methods have allowed insect and other arthropod evidence to become increasingly useful in criminal and civil investigations. however, comprehensive faunal inventories are still needed, including cataloging local dna sequences for forensically significant diptera. this multi-year fly-trapping study was built upon and expanded a previous survey of these flies in santa clara county, including ... | 2015 | 26025701 |
| hydrocarbon profiles throughout adult calliphoridae aging: a promising tool for forensic entomology. | blow flies (diptera: calliphoridae) are typically the first insects to arrive at human remains and carrion. predictable succession patterns and known larval development of necrophagous insects on vertebrate remains can assist a forensic entomologist with estimates of a minimum post-mortem interval (pmimin) range. however, adult blow flies are infrequently used to estimate the pmimin, but rather are used for a confirmation of larval species identification. cuticular hydrocarbons have demonstrated ... | 2014 | 25447177 |
| effects of temperature and tissue type on chrysomya rufifacies (diptera: calliphoridae) (macquart) development. | the hairy maggot blow fly, chrysomya rufifacies (diptera: calliphoridae), is a forensically important fly often encountered on human and other vertebrate remains in temperate and tropic regions throughout the world including australia, asia, central america and north america. c. rufifacies was reared under controlled laboratory conditions on three muscle types (i.e., porcine, equine and canine) at three temperatures (i.e., 20.8, 24.8 and 28.3°c). rate of larval weight gain across time was statis ... | 2014 | 25447170 |
| human wound colonization by lucilia eximia and chrysomya rufifacies (diptera: calliphoridae): myiasis, perimortem, or postmortem colonization? | the infestation of human or animal tissues by fly larvae has been given distinctive terminology depending on the timing and location of colonization. wounds and orifices colonized by diptera in a living human or animal are typically referred to as myiasis. when the colonization occurs after death, it is referred to as postmortem colonization and can be used to estimate the minimum postmortem interval. what happens when the human, as in the case presented here, has a necrotic limb while the human ... | 2014 | 24897868 |
| impact of abiotic factor changes in blowfly, achoetandrus rufifacies (diptera: calliphoridae), in northern thailand. | understanding how medically important flies respond to abiotic factor changes is necessary for predicting their population dynamics. in this study, we investigated the geographical distribution of the medically important blowfly, achoetandrus rufifacies (macquart) (diptera: calliphoridae), and ascertained the response to climatic and physio-environmental factors in chiang mai, northern thailand. adult fly surveys were carried out every 2 weeks from may 2009 to may 2010 at 18 systematically rando ... | 2014 | 24535731 |
| genome sizes of forensically relevant diptera. | genome size estimates for both sexes of forensically relevant diptera from 17 species (four families) are reported herein. average genome sizes ranged from 425.8 mb for female chrysomya rufifacies to 1,197.4 mb for male haematobia irritans. these estimates are useful not only for molecular studies, but also for determination of the species and sex of immatures. species in three of the sampled families had sexually dimorphic genome sizes, presenting a new tool useful for the determination of sex ... | 2012 | 22308788 |
| resource use by an introduced and native carrion flies. | the carrion fly chrysomya rufifacies has recently been introduced to north america. larvae of this species are facultative predators on other carrion larvae, and are known to reduce populations of the new world fly cochliomyia macellaria in the laboratory and in certain field situations. in order to identify conditions under which native taxa might avoid interaction with the invader, we examined broad patterns of resource use by capturing postfeeding larvae as they left a carcass. the calliphori ... | 1994 | 28313964 |
| analysis of the predetermining effect of a sex realizer by ovary transplantations in the monogenic flychrysomya rufifacies. | in the heterozygousf'/f female-producing females of the strictly monogenic blowflychrysomya rufifacies the gene product of the dominant or epistatic female sex realizerf' which causes sexpredetermination is thought to be synthesized either by cells of the germ line (oocytes, nurse cells or oogonia) or by somatic cells and then transferred into the oocytes. to determine the possible site of synthesis, reciprocal transplantations were made of prepupal ovaries between female-producing (thelygenic; ... | 1980 | 28305153 |