| the morphology of nocardia opaca waksman & henrici (proactinomyces opacus jensen) when grown on hydrocarbons, vegetable oils, fatty acids and related substances. | | 1954 | 13221763 |
| beta-oxidation of fatty acids by nocardia opaca. | | 1955 | 13278485 |
| effect of substitution in the side-chain on beta-oxidation of aryloxy-alkylcarboxylic acids by nocardia opaca. | | 1959 | 13644179 |
| production of pentose intermediates during growth of nocardia opaca and other saprophytic soil nocardias and mycobacteria. | | 1959 | 13818462 |
| chromosome topology and genome size of selected actinomycetes species. | information about the genome organization of actinomycetes species is restricted to a few genera: corynebacterium, mycobacterium, rhodococcus, saccharopolyspora and streptomyces. streptomyces species and saccharopolyspora erythraea were shown to contain a single linear 8 mb chromosome. in contrast, the corynebacterium, mycobacterium and rhodococcus species studied were demonstrated to possess a smaller (3 mb-6.5 mb) single circular chromosome. to investigate whether linear chromosome topology an ... | 2000 | 11386344 |
| the metabolism of iron-, zinc- and manganese-deficient nocardia opaca. | | 1962 | 14005472 |
| enzymes of a new modified ortho-pathway utilizing 2-chlorophenol in rhodococcus opacus 1cp. | chlorocatechol 1,2-dioxygenase (cc 1,2-do), chloromuconate cycloisomerase (cmci), chloromuconolactone isomerase (cmli), and dienolactone hydrolase (delh), the key enzymes of a new modified ortho-pathway in rhodococcus opacus 1cp cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway, were isolated and characterized. cc 1,2-do showed the maximum activity with 3-chlorocatechol; its activity with catechol and 4-chlorocatechol was 93 and 50%, respectively. the enzym ... | 2001 | 11405892 |
| factors affecting mass transfer limited biodegradation in saturated porous media. | microbial degradation rates in the subsurface are not only limited by the physiological capacity of the organisms, but also by inefficient supply of nutrients to the microbes. although mass transfer limitation of biodegradation in the subsurface has been postulated for years, experimental evidence is still scarce. in the column experiments described here, diffusive transport of 4-nitroanisole from the bulk solution to cells of rhodococcus opacus strain as2 immobilized on glass beads or sand appe ... | 2001 | 11475163 |
| nitrite elimination and hydrolytic ring cleavage in 2,4,6-trinitrophenol (picric acid) degradation. | two hydrogenation reactions in the initial steps of degradation of 2,4,6-trinitrophenol produce the dihydride meisenheimer complex of 2,4,6-trinitrophenol. the npdh gene (contained in the npd gene cluster of the 2,4,6-trinitrophenol-degrading strain rhodococcus opacus hl pm-1) was shown here to encode a tautomerase, catalyzing a proton shift between the aci-nitro and the nitro forms of the dihydride meisenheimer complex of 2,4,6-trinitrophenol. an enzyme (which eliminated nitrite from the aci-ni ... | 2004 | 15128543 |
| rhodococcus opacus expresses the xsc gene to utilize taurine as a carbon source or as a nitrogen source but not as a sulfur source. | the gram-positive bacteria rhodococcus opacus iso-5 and rhodococcus sp. rha1 utilized taurine (2-aminoethanesulfonate) as the sole source of carbon or of nitrogen or of sulfur for growth. different gene clusters and enzymes were active under these different metabolic situations. under carbon- or nitrogen-limited conditions three enzymes were induced, though to different levels: taurine-pyruvate aminotransferase (tpa), alanine dehydrogenase (ald) and sulfoacetaldehyde acetyltransferase (xsc). the ... | 2004 | 15184572 |
| characterization of rhodococcus opacus r7, a strain able to degrade naphthalene and o-xylene isolated from a polycyclic aromatic hydrocarbon-contaminated soil. | rhodococcus opacus r7 was isolated from a soil contaminated with polycyclic aromatic hydrocarbons for its ability to grow on naphthalene. the strain was also able to degrade o-xylene, the isomer of xylenes most recalcitrant to microbial degradation. the catabolic pathways for naphthalene and o-xylene were investigated by identification of metabolites in r. opacus r7 cultures performed with the two hydrocarbons and by evaluation of some enzymes involved in the metabolism of these compounds. 1,2-d ... | 2001 | 11605984 |
| preparative isolation of lipid inclusions from rhodococcus opacus and rhodococcus ruber and identification of granule-associated proteins. | triacylglycerol granules synthesized and accumulated by rhodococcus opacus and rhodococcus ruber were isolated by glycerol density gradient centrifugation. whereas only one type of granule could be isolated from r. opacus, two types of granules with different specific densities were isolated from r. ruber. both types of r. ruber granules showed a similar content of triacylglycerols and poly(3-hydroxybutyrate- co-3-hydroxyvalerate), but the protein profiles of both types were significantly differ ... | 2001 | 11797040 |
| expression of a functional nad-reducing [nife] hydrogenase from the gram-positive rhodococcus opacus in the gram-negative ralstonia eutropha. | the actinomycete rhodococcus opacus mr11 harbors a bidirectional nad-reducing [nife] hydrogenase (sh). this cytoplasmic enzyme is composed of two heterodimeric modules which catalyze distinct enzymatic activities. the hydrogenase moiety mediates h(2):benzyl viologen oxidoreductase activity and the fmn-containing diaphorase module displays nadh:benzyl viologen oxidoreductase activity. the sh of rh. opacus resembles [nife] hydrogenases present in strains of the proteobacterium ralstonia eutropha a ... | 2002 | 11807565 |
| npd gene functions of rhodococcus (opacus) erythropolis hl pm-1 in the initial steps of 2,4,6-trinitrophenol degradation. | rhodococcus (opacus) erythropolis hl pm-1 grows on 2,4,6-trinitrophenol (picric acid) or 2,4-dinitrophenol (2,4-dnp) as sole nitrogen source. a gene cluster involved in picric acid degradation was recently identified. the functional assignment of three of its genes, npdc, npdg and npdi, and the tentative functional assignment of a fourth one, npdh, is reported. the genes were expressed in escherichia coli as his-tag fusion proteins that were purified by ni-affinity chromatography. the enzyme act ... | 2002 | 11882715 |
| preliminary crystallographic analysis of 3-chlorocatechol 1,2-dioxygenase of a new modified ortho-pathway from the gram-positive rhodococcus opacus 1cp grown on 2-chlorophenol. | 3-chlorocatechol 1,2-dioxygenase (3-clc1,2do), a key enzyme of a new modified ortho-pathway, was isolated from a variant of the gram-positive bacterium rhodococcus opacus 1cp utilizing 2-chlorophenol as the sole energy and carbon source via a 3-chlorocatechol branch of a modified ortho-pathway. 3-clc1,2do catalyzes the intradiol cleavage of 3-chlorocatechol. the enzyme contains fe(iii) ions essential to the catalytic activity; it is a homodimer with a molecular weight of about 58 kda composed of ... | 2003 | 12499567 |
| rhodococcus jostii sp. nov., isolated from a medieval grave. | the taxonomic position of a bacterial strain isolated from the femur of the remains of jost lucemburský, margrave in moravia, brno (czech republic), was investigated by phenotypic, chemotaxonomic and molecular taxonomic methods. the chemotaxonomic characteristics, including the cell-wall amino acid and sugar compositions, the quinone system and the fatty acid profile, were in good agreement with those of the genus rhodococcus. the g+c content of the dna was 67.4 mol%. comparative 16s rrna gene s ... | 2002 | 11931149 |
| identification of phenyldecanoic acid as a constituent of triacylglycerols and wax ester produced by rhodococcus opacus pd630. | phenyldecane supported growth and lipid accumulation of rhodococcus opacus pd630 during cultivation under nitrogen-limiting conditions. the results of this study suggested that the hydrocarbon phenyldecane was degraded by monoterminal oxidation, followed by beta-oxidation of the alkyl side-chain to phenylacetic acid, and by an additional degradative route for the oxidation of the latter to intermediates of the central metabolism. alpha-oxidation of phenyldecanoic acid also occurred to some exten ... | 2002 | 11988514 |
| metabolism of dibenzo-p-dioxin and chlorinated dibenzo-p-dioxin by a gram-positive bacterium, rhodococcus opacus sao101. | a dibenzo-p-dioxin-degrading bacterial strain, rhodococcus opacus sao101, was isolated from forest soil samples collected from the subtropical islands of japan by enrichment of a mineral salt medium containing dibenzofuran as the sole carbon and energy source. the isolated bacterium could utilize dibenzo-p-dioxin as the sole carbon and energy source, and also many monocyclic aromatic compounds, such as toluene, phenol, and chlorobenzene, as well as bicyclic aromatic compounds, such as biphenyl, ... | 2001 | 16233073 |
| heterologous expression of rhodococcus opacus l-amino acid oxidase in streptomyces lividans. | l-amino acid oxidase (l-aao) from rhodococcus opacus is a highly enantioselective enzyme with a broad substrate specificity that catalyses the oxidation of l-amino acids to keto acids. the lao-gene (ay053450) from r. opacus was cloned into different escherichia coli and streptomyces lividans expression vectors. expression in e. coli resulted in the accumulation of insoluble protein, but s. lividans was a suitable host for the heterologous production of l-aao. when using the thiostrepton-inducibl ... | 2003 | 12699695 |
| homologous npdgi genes in 2,4-dinitrophenol- and 4-nitrophenol-degrading rhodococcus spp. | rhodococcus (opacus) erythropolis hl pm-1 grows on 2,4,6-trinitrophenol or 2,4-dinitrophenol (2,4-dnp) as a sole nitrogen source. the nadph-dependent f(420) reductase (ndfr; encoded by npdg) and the hydride transferase ii (htii; encoded by npdi) of the strain were previously shown to convert both nitrophenols to their respective hydride meisenheimer complexes. in the present study, npdg and npdi were amplified from six 2,4-dnp degrading rhodococcus spp. the genes showed sequence similarities of ... | 2003 | 12732545 |
| conversion of 2-fluoromuconate to cis-dienelactone by purified enzymes of rhodococcus opacus 1cp. | the present study describes the (19)f nuclear magnetic resonance analysis of the conversion of 3-halocatechols to lactones by purified chlorocatechol 1,2-dioxygenase (clca2), chloromuconate cycloisomerase (clcb2), and chloromuconolactone dehalogenase (clcf) from rhodococcus opacus 1cp grown on 2-chlorophenol. the 3-halocatechol substrates were produced from the corresponding 2-halophenols by either phenol hydroxylase from trichosporon cutaneum or 2-hydroxybiphenyl 3-mono-oxygenase from pseudomon ... | 2003 | 12957954 |
| [heterogeneity of rhodococcus opacus 1cp as a response to stress induced by chlorophenols]. | dissociation of rhodococcus opacus 1cp during culturing in different media (containing phenol and its monochlorinated derivatives as the sole source of carbon and energy) was studied. three variants of strain 1cp (s1, s2, and r) differing in the morphology of cells and colonies, lipid composition, and manner of growth on phenol and monochlorophenols were isolated. it was shown that 2- and 4-chlorophenols were most actively degraded by the smooth (s) forms of the culture, and that the rough (r) f ... | 2005 | 16240653 |
| the metabolism of some saturated aliphatic hydrocarbons, alcohols and fatty acids by proactinomyces opacus jensen (nocardia opaca waksman & hendrik). | | 1952 | 12977738 |
| [dependence of transformation of chlorophenols by rhodococci on position and number of chlorine atoms in the aromatic ring]. | study of the conversion of chlorophenols by rhodococcus opacus 1g, r. rhodnii 135, r. rhodochrous 89, and r. opacus 1cp disclosed the dependence of the conversion rate and pathway on the number and position of chlorine atoms in the aromatic ring. the most active chlorophenol converter, strain r. opacus 1cp, grew on each of the three isomeric monochlorophenols and on 2,4-dichlorophenol; the rate of growth decreased from 4-chlorophenol to 3-chlorophenol and then to 2-chlorophenol. the parameters o ... | 2000 | 10808489 |
| rhodococcus opacus strain pd630 as a new source of high-value single-cell oil? isolation and characterization of triacylglycerols and other storage lipids. | the triacylglycerol (tag)-accumulating, hydrocarbon-degrading bacterium rhodococcus opacus strain pd630 and chemically induced storage-deficient mutants derived from this strain were investigated for their capability to accumulate storage lipids in the cytoplasm during cultivation under nitrogen-limiting conditions. acylglycerols were analysed by matrix-associated laser desorption ionization-time of flight mass spectrometry (maldi-tof) and by reversed-phase hplc. fatty acids comprising 13-19 car ... | 2000 | 10832641 |
| accumulation and mobilization of storage lipids by rhodococcus opacus pd630 and rhodococcus ruber ncimb 40126. | the time course of the accumulation of triacylglycerols (tags) in rhodococcus opacus pd630 or of tags plus polyhydroxyalkanoates (pha) in rhodococcus ruber ncimb 40126 with gluconate or glucose as carbon source, respectively, was studied. in addition, we examined the mobilization of these storage compounds in the absence of a carbon source. r. opacus accumulated tags only after the exhaustion of ammonium in the medium, and, with a fixed concentration of the carbon source, the amounts of tags in ... | 2000 | 10968636 |
| crystallization and preliminary x-ray analysis of a bacterial l-amino-acid oxidase from rhodococcus opacus. | l-amino-acid oxidases (ec 1.4.3.2) catalyse the stereospecific oxidative deamination of an l-amino-acid substrate to an alpha-keto acid with the production of ammonia and hydrogen peroxide. in this study, the crystallization and preliminary x-ray analysis of a bacterial l-amino-acid oxidase from rhodococcus opacus (rolaao) is described. rolaao is a dimeric protein consisting of two identical subunits of 489 amino acids with a calculated molecular weight of 54.2 kda and a non-covalently bound fad ... | 2006 | 16511322 |
| in vitro effects of sterculic acid on lipid biosynthesis in rhodococcus opacus strain pd630 and isolation of mutants defective in fatty acid desaturation. | the in vivo effects of sterculic acid methyl ester on triacylglycerol fatty acid composition in the oleaginous, hydrocarbon-degrading bacterium r. opacus strain pd630 was investigated. sterculic acid, a cyclopropene fatty acid and an inhibitor of the stearoyl-coa desaturase system, strongly inhibited the synthesis of monoenic fatty acids, of saturated fatty acids with more than 16 carbon atoms and of odd-numbered fatty acids when added to the culture medium. in addition, chemical mutagenesis and ... | 2000 | 10981688 |
| [growth of rhodococcus opacus on mixtures of monohalogenated benzenes and phenols]. | the growth of rhodococcus opacus gm-14 on mixtures of 2-chloro- and 2-bromophenol, of 4-chloro, 4-bromo-, and 4-iodophenol, and of chloro-, bromo-, and iodobenzenes was accompanied by consumption of the substrates and excretion of halogen ions to the medium. during the growth on monochlorophenols, the substrates were consumed sequentially in the following order: 4-chloro-, 3-chloro-, and then 2-chlorophenol. chlorine ions were excreted in a two-phase manner in amounts comprising 79% of the theor ... | 2000 | 11008684 |
| 4-chlorocatechol 1,2-dioxygenase from the chlorophenol-utilizing gram-positive rhodococcus opacus 1cp: crystallization and preliminary crystallographic analysis. | 4-chlorocatechol 1,2-dioxygenase (4-clc1,2do) from the gram-positive bacterium rhodococcus opacus (erythropolis) 1cp, an enzyme involved in the aerobic biodegradation of chloroaromatic compounds, has been crystallized. 4-clc1,2do, which specifically catalyzes the intradiol cleavage of 4-substituted catechols, which are intermediates in the degradation of a variety of aromatic pollutants, to the corresponding maleylacetates, has recently been purified to homogeneity. the enzyme is an homodimer co ... | 2002 | 12037322 |
| a pharmacokinetic study of jomo-tech in rats. | a pharmacokinetic study of 99mtc labelled jomo-tech in rats (after intravenous administration of a dose of 20 microg/kg body weight) was conducted. jomo-tech is a heterogeneous extract derived from nocardia opaca cell walls. an excellent fitting of the three-compartmental disposition model was achieved. the first apparent elimination half-life was very short (t1/2alpha = 0.0572 +/- 0.01383 h) followed by longer second apparent elimination half-life (t1/2beta = 0.817 +/- 0.1922 h), whereas at lat ... | 2000 | 11112087 |
| eukaryotic lipid body proteins in oleogenous actinomycetes and their targeting to intracellular triacylglycerol inclusions: impact on models of lipid body biogenesis. | bacterial neutral lipid inclusions are structurally related to eukaryotic lipid bodies. these lipid inclusions are composed of a matrix of triacylglycerols (tags) or wax esters surrounded by a monolayer of phospholipids. whereas the monolayers of lipid bodies from animal and plant cells harbor specific classes of proteins which are involved in the structure of the inclusions and lipid homoestasis, no such proteins are known to be associated with bacterial lipid inclusions. the present study was ... | 2006 | 17021226 |
| transfer of tsukamurella wratislaviensis goodfellow et a. 1995 to the genus rhodococcus as rhodococcus wratislaviensis comb. nov.. | a polyphasic study was undertaken to clarify the taxonomic position of the type strain (n805t) of tsukamurella wratislaviensis. this organism showed a combination of phenotypic properties, notably chemotaxonomic markers, consistent with its classification in the genus rhodococcus. comparative 16s rdna sequencing studies indicated that strain 805t falls into the rhodococcus erythropolis subclade, where it forms a monophyletic group with the type strains of rhodococcus opacus and rhodococcus perco ... | 2002 | 12054234 |
| triacylglycerols in prokaryotic microorganisms. | triacylglycerols (tag) are fatty acid triesters of glycerol; there are diverse types of tag with different properties depending on their fatty acid composition. the occurrence of tag as reserve compounds is widespread among eukaryotic organisms such as yeast, fungi, plants and animals, whereas occurrence of tag in bacteria has only rarely been described. however, accumulation of tag seems to be widespread among bacteria belonging to the actinomycetes group, such as species of mycobacterium, stre ... | 2002 | 12466875 |
| the ralstonia eutropha h16 phasin phap1 is targeted to intracellular triacylglycerol inclusions in rhodococcus opacus pd630 and mycobacterium smegmatis mc2155, and provides an anchor to target other proteins. | in ralstonia eutropha, the h16 phasin phap1 represents the major phasin that binds to the surface of polyhydroxyalkanoate (pha) inclusions. in this study, c-terminal fusions of phap1 with enhanced green fluorescent protein (egfp) and with escherichia coli beta-galactosidase (lacz) were expressed separately in the triacylglycerol (tag)-accumulating actinomycetes rhodococcus opacus pd630 and mycobacterium smegmatis mc(2)155, employing the m. smegmatis acetamidase (ace) promoter of the escherichia- ... | 2006 | 17074898 |
| protective effect of nocardia opaca lysozyme digest in experimental murine candida albicans infections. | candida albicans, as an opportunistic pathogen, causes therapeutic problems in immunocompetent individuals and frequently it initiates severe infections in immunocompromised hosts. the application of a lysozyme digest preparation from the cell walls of nocardia opaca (nocardia lysozyme digest; nld), recently classified as rhodococcus opacus, has a protective effect in intravenous (i.v.) c. albicans infections in inbred icr mice which have normal complement production. it also significantly reduc ... | 2001 | 11346272 |
| npdr, a repressor involved in 2,4,6-trinitrophenol degradation in rhodococcus opacus hl pm-1. | rhodococcus opacus hl pm-1 utilizes 2,4,6-trinitrophenol (picric acid) as a sole nitrogen source. the initial attack on picric acid occurs through two hydrogenation reactions. hydride transferase ii (encoded by npdi) and hydride transferase i (encoded by npdc) are responsible for the hydride transfers. database searches with the npd genes have indicated the presence of a putative transcriptional regulator, npdr. here, the npdr gene was expressed in escherichia coli, and the protein was purified ... | 2004 | 14679229 |
| crystal structure of 4-chlorocatechol 1,2-dioxygenase from the chlorophenol-utilizing gram-positive rhodococcus opacus 1cp. | the crystal structure of the 4-chlorocatechol 1,2-dioxygenase from the gram-positive bacterium rhodococcus opacus (erythropolis) 1cp, a fe(iii) ion-containing enzyme involved in the aerobic biodegradation of chloroaromatic compounds, has been solved by multiple wavelength anomalous dispersion using the weak anomalous signal of the two catalytic irons (1 fe/257 amino acids) and refined at a 2.5 a resolution (r(free) 28.7%; r factor 21.4%). the analysis of the structure and its comparison with the ... | 2004 | 15060064 |
| a novel p-nitrophenol degradation gene cluster from a gram-positive bacterium, rhodococcus opacus sao101. | p-nitrophenol (4-np) is recognized as an environmental contaminant; it is used primarily for manufacturing medicines and pesticides. to date, several 4-np-degrading bacteria have been isolated; however, the genetic information remains very limited. in this study, a novel 4-np degradation gene cluster from a gram-positive bacterium, rhodococcus opacus sao101, was identified and characterized. the deduced amino acid sequences of npcb, npca, and npcc showed identity with phenol 2-hydroxylase compon ... | 2004 | 15262926 |
| isolation and characterization of the rhodococcus opacus thiostrepton-inducible genes tipal and tipas: application for recombinant protein expression in rhodococcus. | we cloned the rhodococcus opacus (strain dsm 44193) tipa gene, which encodes two translation products, tipal and tipas. the gene products are homologous to the streptomyces spp. tipal and tipas proteins, respectively. the tipa promoter is highly active and tipas protein is predominantly accumulated in r. opacus cells when the inducer of transcription, thiostrepton, was presented in culture medium. we found that thiostrepton is also induced the expression of an endogenous tipa-family protein in r ... | 2004 | 15268935 |
| a linear megaplasmid, p1cp, carrying the genes for chlorocatechol catabolism of rhodococcus opacus 1cp. | the gram-positive actinobacterium rhodococcus opacus 1cp is able to utilize several (chloro)aromatic compounds as sole carbon sources, and gene clusters for various catabolic enzymes and pathways have previously been identified. pulsed-field gel electrophoresis indicates the occurrence of a 740 kb megaplasmid, designated p1cp. linear topology and the presence of covalently bound proteins were shown by the unchanged electrophoretic mobility after s1 nuclease treatment and by the immobility of the ... | 2004 | 15347765 |
| mechanism of lipid-body formation in prokaryotes: how bacteria fatten up. | neutral lipid accumulation is frequently observed in some gram-negative prokaryotes like acinetobacter sp. and most actinomycetes, including the pathogenic mycobacterium tuberculosis and antibiotic producing streptomycetes. we examined the formation of wax ester- and triacylglycerol (tag)-bodies in acinetobacter calcoaceticus and rhodococcus opacus using microscopic, immunological and biophysical methods. a general model for prokaryotic lipid-body formation is proposed, clearly differing from th ... | 2005 | 15661001 |
| degradation of 5-nitroguaiacol by soil bacteria of the genus rhodococcus. | two bacterial strains were isolated from forest soil by selective enrichment of the mineral medium containing 4-nitropyrocatechol as the sole carbon and energy source. both strains could utilize 4-nitropyrocatechol and 5-nitroguaiacol. degradation of 5-nitroguaiacol and stoichiometric release of nitrites was measured during its degradation both in growing culture and for resting cells. both strains were unable to degrade other nitroaromatic compounds such as 4-nitroguaiacol, 2-nitrophenol, 3-nit ... | 2004 | 15702555 |
| differences of heterotrophic 13co2 assimilation by pseudomonas knackmussii strain b13 and rhodococcus opacus 1cp and potential impact on biomarker stable isotope probing. | motivated by the finding that pseudomonas knackmussii b13 but not rhodococcus opacus 1cp grows in the absence of externally provided co(2), we investigated the assimilation of (13)co(2) into active cells cultivated with non-labelled glucose as sole energy substrate. (13)c found in the bulk biomass indicated a substantial but different co(2) assimilation by pseudomonas and rhodococcus, respectively (3500 per thousand and 2600 per thousand). cellular fatty acids were labelled from -15 per thousand ... | 2008 | 18341583 |
| crystal structure of the hydroxyquinol 1,2-dioxygenase from nocardioides simplex 3e, a key enzyme involved in polychlorinated aromatics biodegradation. | hydroxyquinol 1,2-dioxygenase (1,2-hqd) catalyzes the ring cleavage of hydroxyquinol (1,2,4-trihydroxybenzene), a central intermediate in the degradation of aromatic compounds including a variety of particularly recalcitrant polychloro- and nitroaromatic pollutants. we report here the primary sequence determination and the analysis of the crystal structure of the 1,2-hqd from nocardioides simplex 3e solved at 1.75 a resolution using the multiple wavelength anomalous dispersion of the two catalyt ... | 2005 | 15772073 |
| identification and structural characterisation of novel trehalose dinocardiomycolates from n-alkane-grown rhodococcus opacus 1cp. | rhodococcus opacus 1cp, a potent degrader of (chloro-) aromatic compounds was found to utilise c10-c16 n-alkanes as sole carbon sources. highest conversion rates were observed with n-tetradecane and n-hexadecane, whereas the utilisation of n-dodecane and n-decane was considerably slower. thin-layer chromatography of organic extracts of n-alkane-grown 1cp cultures indicated the growth-associated formation of a glycolipid which was characterised as a trehalose dimycolate by 1h-nmr spectroscopy and ... | 2006 | 16133336 |
| difference in degrading p-nitrophenol between indigenous bacteria in a reactor. | p-nitrophenol (pnp) -degrading bacteria were isolated from a reactor using a mineral salt medium containing a low and high pnp concentration. we isolated two bacterial species, pseudomonas sp. ytk17 and rhodococcus opacus ytk32, that utilize pnp as their sole source of carbon and energy. these strains exhibited differences in pnp degradation activity in relation to pnp concentration. strain ytk17 showed a high level of degradation following pre-exposure to a low pnp concentration, whereas strain ... | 2002 | 16233242 |
| isolation and characterization of benzene-tolerant rhodococcus opacus strains. | twenty-two benzene-utilizing bacteria were isolated from soil samples. among them, three isolates were highly tolerant to benzene. they grew on benzene when liquid benzene was added to the basal salt medium at 10--90% (v/v). taxonomical analysis identified the benzene-tolerant isolates as rhodococcus opacus. one of the benzene-tolerant isolates, designated b-4, could utilize many aromatic and aliphatic hydrocarbons including benzene, toluene, styrene, xylene, ethylbenzene, propylbenzene, n-octan ... | 2005 | 16233805 |
| development of a genetic transformation system for benzene-tolerant rhodococcus opacus strains. | rhodococcus opacus b-4 and b-9 are tolerant to various organic solvents including benzene, toluene, ethylbenzene, xylenes and styrene, and are suitable bacterial hosts for the production of chemical products from hydrophobic substrates. a 4.4-kb endogenous plasmid (pknr 01) was isolated from r. opacus b-4 and sequenced completely. plasmid pknr 01 encodes proteins that share similarity to replication proteins from the enteric bacterial and actinomycete theta-replication plasmids. a 7.4-kb chimeri ... | 2005 | 16233810 |
| characterization of four rhodococcus alcohol dehydrogenase genes responsible for the oxidation of aromatic alcohols. | four genes were isolated and characterized for alcohol dehydrogenases (adhs) catalyzing the oxidation of aromatic alcohols such as benzyl alcohol to their corresponding aldehydes, one from o-xylene-degrading rhodococcus opacus tkn14 and the other three from n-alkane-degrading rhodococcus erythropolis pr4. various aromatic alcohols were bioconverted to their corresponding carboxylic acids using escherichia coli cells expressing each of the four adh genes together with an aromatic aldehyde dehydro ... | 2006 | 16292529 |
| isolation and characterization of o-xylene oxygenase genes from rhodococcus opacus tkn14. | o-xylene is one of the most difficult-to-degrade environmental pollutants. we report here rhodococcus genes mediating oxygenation in the first step of o-xylene degradation. rhodococcus opacus tkn14, isolated from soil contaminated with o-xylene, was able to utilize o-xylene as the sole carbon source and to metabolize it to o-methylbenzoic acid. a cosmid library from the genome of this strain was constructed in escherichia coli. a bioconversion analysis revealed that a cosmid clone incorporating ... | 2005 | 16332743 |
| the effect of deficiency of iron, zinc and manganese on the growth and morphology of nocardia opaca. | | 1960 | 13843324 |
| potential of rhodococcus strains for biotechnological vanillin production from ferulic acid and eugenol. | the potential of two rhodococcus strains for biotechnological vanillin production from ferulic acid and eugenol was investigated. genome sequence data of rhodococcus sp. i24 suggested a coenzyme a-dependent, non-beta-oxidative pathway for ferulic acid bioconversion, which involves feruloyl-coa synthetase (fcs), enoyl-coa hydratase/aldolase (ech), and vanillin dehydrogenase (vdh). this pathway was proven for rhodococcus opacus pd630 by physiological characterization of knockout mutants. however, ... | 2006 | 16421716 |
| identification of a novel self-sufficient styrene monooxygenase from rhodococcus opacus 1cp. | sequence analysis of a 9-kb genomic fragment of the actinobacterium rhodococcus opacus 1cp led to identification of an open reading frame encoding a novel fusion protein, stya2b, with a putative function in styrene metabolism via styrene oxide and phenylacetic acid. gene cluster analysis indicated that the highly related fusion proteins of nocardia farcinica ifm10152 and arthrobacter aurescens tc1 are involved in a similar physiological process. whereas 413 amino acids of the n terminus of stya2 ... | 2009 | 19482928 |
| identification and characterization of o-xylene-degrading rhodococcus spp. which were dominant species in the remediation of o-xylene-contaminated soils. | soils contaminated with o-xylene were more difficult to bioremediate than those contaminated with other btex hydrocarbons (benzene, toluene, ethylbenzene, m-xylene and p-xylene). in order to identify microorganisms responsible for o-xylene degradation in soil, microbial community structure analyses were carried out with two soil samples in the presence of o-xylene and mineral nutrients. in two different soil samples, rhodococcus opacus became abundant. we were also able to isolate o-xylene degra ... | 2007 | 16485082 |
| utilization of halogenated benzenes, phenols, and benzoates by rhodococcus opacus gm-14. | strain gm-14 was isolated by selective enrichment from contaminated soil with chlorobenzene as the sole source of carbon and energy. it utilizes an exceptionally wide spectrum of haloaromatic substrates. it is a gram-positive, weakly acid-fast actinomycete, with a morphological cycle from cocci and short rods to long rods and branched filaments; it grew optimally at 28(deg)c; and it tolerated 5% nacl in rich medium. the chemotaxonomic characteristics, the diagnostic biochemical tests, the whole- ... | 1995 | 16535177 |
| genetic and biochemical characterization of the dioxygenase involved in lateral dioxygenation of dibenzofuran from rhodococcus opacus strain sao101. | rhodococcus opacus strain sao101 was shown to degrade on various polycyclic aromatic hydrocarbons such as naphthalene, dibenzofuran (df), and dibenzo-p-dioxin (dd). one of the unique traits of the strain sao101 is its ability to oxidize df compounds by lateral dioxygenation. to clone the lateral dioxygenase gene involved in compound degradation in strain sao101, we identified a cosmid clone that oxidizes aromatic compounds by using sao101 genomic dna. sequencing analysis revealed that isolated c ... | 2006 | 16736088 |
| crystal structure of 3-chlorocatechol 1,2-dioxygenase key enzyme of a new modified ortho-pathway from the gram-positive rhodococcus opacus 1cp grown on 2-chlorophenol. | the crystal structure of the 3-chlorocatechol 1,2-dioxygenase from the gram-positive bacterium rhodococcus opacus (erythropolis) 1cp, a fe(iii) ion-containing enzyme specialized in the aerobic biodegradation of 3-chloro- and methyl-substituted catechols, has been solved by molecular replacement techniques using the coordinates of 4-chlorocatechol 1,2-dioxygenase from the same organism (pdb code 1s9a) as a starting model and refined at 1.9 a resolution (r(free) 21.9%; r-factor 17.4%). the analysi ... | 2006 | 16793061 |
| rhodococcus imtechensis sp. nov., a nitrophenol-degrading actinomycete. | a gram-positive actinobacterium, strain rkj300(t), capable of utilizing p-nitrophenol and 2,4-dinitrophenol, was isolated from a pesticide-contaminated site in india. the morphological and chemotaxonomic properties of the isolate were typical of members of the genus rhodococcus. the dna g+c content was 72 mol%. strain rkj300(t) exhibited the highest level of sequence similarity with rhodococcus wratislaviensis ncimb 13082(t) (99.3 %), followed by rhodococcus opacus dsm 43205(t) (98.8 %), rhodoco ... | 2006 | 16902038 |
| analysis of the 7.6-kb cryptic plasmid pnc500 from rhodococcus rhodochrous b-276 and construction of rhodococcus-e. coli shuttle vector. | four circular cryptic plasmids were detected from propene-degrading rhodococcus rhodochrous (formerly nocardia corallina) b-276 and the smallest 7.6-kb plasmid, named pnc500 was used to construct rhodococcus-e. coli shuttle vector, pnc5403. sequence analysis of pnc500 revealed that the plasmid contains eight potential orfs, namely 1 through 8. the deduced amino acid sequences for orfs 3, 4, 6, and 7 show homology with those of rep a, rep b, dna methyl-transferase (m.xami), and restriction nuclea ... | 2007 | 17043815 |
| cloning, sequencing, and expression of a novel epoxide hydrolase gene from rhodococcus opacus in escherichia coli and characterization of enzyme. | an epoxide hydrolase gene of about 0.8 kb was cloned from rhodococcus opacus ml-0004, and the open reading frame (orf) sequence predicted a protein of 253 amino acids with a molecular mass of about 28 kda. an expression plasmid carrying the gene under the control of the tac promotor was introduced into escherichia coli, and the epoxide hydrolase gene was successfully expressed in the recombinant strains. some characteristics of purified recombinant epoxide hydrolase were also studied. epoxide hy ... | 2007 | 17043819 |
| specificity of catechol ortho-cleavage during para-toluate degradation by rhodococcus opacus 1cp. | degradation of para-toluate by rhodococcus opacus 1cp was investigated. activities of the key enzymes of this process, catechol 1,2-dioxygenase and muconate cycloisomerase, are detected in this microorganism. growth on p-toluate was accompanied by induction of two catechol 1,2-dioxygenases. the substrate specificity and physicochemical properties of one enzyme are identical to those of chlorocatechol 1,2-dioxygenase; induction of the latter enzyme was observed during r. opacus 1cp growth on 4-ch ... | 2006 | 17223783 |
| the structure of a bacterial l-amino acid oxidase from rhodococcus opacus gives new evidence for the hydride mechanism for dehydrogenation. | l-amino acid oxidase from rhodococcus opacus (rolaao) is classified as a member of the gr(2)-family of flavin-dependent oxidoreductases according to a highly conserved sequence motif for the cofactor binding. the monomer of the homodimeric enzyme consists of three well-defined domains: the fad-binding domain corresponding to a general topology throughout the whole gr(2)-family; a substrate-binding domain with almost the same topology as the snake venom laao and a helical domain exclusively respo ... | 2007 | 17234209 |
| the rhodococcus opacus pd630 heparin-binding hemagglutinin homolog tada mediates lipid body formation. | generally, prokaryotes store carbon as polyhydroxyalkanoate, starch, or glycogen. the gram-positive actinomycete rhodococcus opacus strain pd630 is noteworthy in that it stores carbon in the form of triacylglycerol (tag). several studies have demonstrated that r. opacus pd630 can accumulate up to 76% of its cell dry weight as tag when grown under nitrogen-limiting conditions. while this process is well studied, the underlying molecular and biochemical mechanisms leading to tag biosynthesis and s ... | 2010 | 20851968 |
| an aerobic sequencing batch reactor for 2,4,6-trinitrophenol (picric acid) biodegradation. | a bench scale sequencing batch reactor (sbr) was designed and tested for degradation of 2,4,6-trinitrophenol (tnp) or picric acid-contaminated wastewater or groundwater. under varying temperatures (25, 15 and 10 degrees c) and influent concentrations (40-200 mg/l tnp) a stable biomass was developed that was consistently capable of degrading the explosive compound to below regulatory drinking water limits (0.057 mg/l). the reactor was initially seeded with a nitroaromatic and nitramine degrading ... | 2007 | 17286267 |
| intradiol pathway of para-cresol conversion by rhodococcus opacus 1cp. | the growth of rhodococcus opacus 1cp in medium with different concentrations of p-cresol as the sole source of carbon and energy was studied. it was shown that the optimal concentration of p-cresol was 600 mg/l. the ability of this strain to transform practically all amounts of p-cresol to 4-methylcatechol followed by its utilization through ortho-pathway was shown. new enzymes (4-methylcatechol 1,2-dioxygenase, catechol 1,2-dioxygenase, and methylmuconate cycloisomerase) were purified to homoge ... | 2007 | 17506026 |
| transfer of megaplasmid pkb1 from the rubber-degrading bacterium gordonia westfalica strain kb1 to related bacteria and its modification. | because engineering of the 101.016-bp megaplasmid pkb1 of gordonia westfalica kb1 failed due to the absence of an effective transfer system, pkb1 was transferred by conjugation from g. westfalica kb1 to a kanamycin-resistant mutant of rhodococcus opacus pd630 at a frequency of about 6.2 x 10(-8) events per recipient cell. furthermore, pkb1 was transferred to g. polyisoprenivorans strains vh2 and y2k and to mycobacterium smegmatis by electroporation at frequencies of 5.5 x 10(3), 1.9 x 10(3), and ... | 2008 | 18034340 |
| stabilization of water-in-oil emulsion by rhodococcus opacus b-4 and its application to biotransformation. | rhodococcus opacus b-4, which has recently been isolated as an organic solvent-tolerant bacterium, stabilized water-in-oil (w/o) emulsions by inhibition of droplet coalescence when the cells were dispersed in 90% (v/v) organic solvents. confocal microscopy revealed that many bacterial cells assembled at the interface between oil and water droplets, though free cells were also detectable at the inside of water droplets. bacterial cells in the w/o emulsion were capable of utilizing both a water-so ... | 2008 | 18270698 |
| repeated batch production of l-phenylalanine from phenylpyruvate and nh(4)cl by immobilized cells of nocardia opaca under hydrogen high pressure. | among various microbial cells examined under screening conditions, nocardia opaca showed the highest activity for production of phenylalanine from phenylpyruvate. here nh(4)cl as well as amino acids were used as an amino donor for phenylalanine production. the phenylalanine production rate increased with increasing hydrogen pressure. the specific activity of phenylalanine dehydrogenase was increased by culturing n. opaca cells in nutrient broth containing 0.3% phenylalanine. as a result, the phe ... | 1988 | 18584688 |
| site-directed mutagenesis of epoxide hydrolase to probe catalytic amino acid residues and reaction mechanism. | epoxide hydrolase from rhodococcus opacus catalyzes the stereospecific hydrolysis of cis-epoxysuccinate to l(+)-tartrate. it shows low but significant similarity to haloacid dehalogenase and haloacetate dehalogenase (16-23% identity). to identify catalytically important residues, we mutated 29 highly conserved charged and polar amino acid residues (except for one alanine). the replacement of d18, d193, r55, k164, h190, t22, y170, n134 and a188 led to a significant loss in the enzyme activity, in ... | 2011 | 21763314 |
| cloning and characterization of a gene involved in triacylglycerol biosynthesis and identification of additional homologous genes in the oleaginous bacterium rhodococcus opacus pd630. | the oleaginous bacterium rhodococus opacus strain pd630 serves as a model organism to investigate the metabolism of storage triacylglycerols (tags) in bacteria. the key enzyme catalysing the last step of tag biosynthesis in bacteria is a promiscuous acyltransferase (atf), exhibiting acyl-coa acyltransferase activity to both diacylglycerols (dgat activity) and fatty alcohols (wax ester synthase, ws activity). an 800 bp pcr product was obtained from chromosomal dna of strain pd630 by using degener ... | 2008 | 18667565 |
| expression of rhodococcus opacus alkb genes in anhydrous organic solvents. | rhodococcus opacus b-4 is a benzene-tolerant bacterium which was isolated from a gasoline-contaminated soil sample. we previously demonstrated that this organism was able to survive and exhibit biocatalytic activity in anhydrous organic solvents for at least 5 d. in the present study, we cloned the alkb1 and alkb2 genes encoding alkane hydroxylases from r. opacus b-4. heterologous expression of the alkb1 and alkb2 genes in escherichia coli jm109 showed that they encode functional alkane hydroxyl ... | 2008 | 18804065 |
| a comparison of various methods to predict bacterial predilection for organic solvents used as reaction media. | bacterial predilection for organic solvents is important in whole-cell biocatalysis in organic media. although various methods of measuring bacterial hydrophobicity have been proposed, it is not fully determined whether they are applicable to the assessment of bacterial predilection for organic solvents in whole-cell biocatalytic processes. in this study, bacterial predilection for organic solvents was assessed by bacterial adhesion to hydrocarbon (bath), contact angle measurement (cam), hydroph ... | 2008 | 19000611 |
| isolation and properties of a 2-chlorovinylarsonic acid-degrading microorganism. | 2-chlorovinylarsonic acid (cvaoa) is a stable abiotic metabolite of lewisite 1 that has been identified in lewisite dumps. there have been no reports of microbial degradation of cvaoa, so we isolated and examined cvaoa-degrading microorganisms. cvaoa contains arsine, which is toxic to microbial growth. we therefore used the simple organic chemical, ethylene, as a sole carbon source in initial screening for suitable microbes. we isolated several microorganisms from sewage sludge and soil. two str ... | 2009 | 19022568 |
| evaluation of toluene degradation pathways by two-dimensional stable isotope fractionation. | toluene degradation by several pure and mixed microbial cultures was investigated bytwo-dimensional compound specific isotope analysis (2d-csia). for most of the cultures, the respective toluene degradation pathway and toluene attacking enzymatic step was known. the slope of the linear regression for hydrogen (delta delta(2)h) vs. carbon (delta delta(13)c) discrimination (lamda = delta delta(2)h/ delta delta(13)c approximately epsilonh(bulk)/epsilonc(bulk)) was determined in order to characteriz ... | 2008 | 19031862 |
| Bioconversion of lignin model compounds with oleaginous Rhodococci. | Although economically efficient biomass conversion depends on the utilization of the complete cell wall (biorefinery concept), including polysaccharides and lignin, current biofuels research concentrate mostly on cellulose conversion, while lignin is viewed as a side-product that is used primarily as a thermal resource. Microbiological conversion of lignin is almost exclusive to fungi, usually resulting in increased cell mass and lignolytic enzymes. Some bacteria can also degrade lignin-related ... | 2011 | 22159607 |
| sulfonate desulfurization in rhodococcus from wheat rhizosphere communities. | organically bound sulfur makes up about 90% of the total sulfur in soils, with sulfonates often the dominant fraction. actinobacteria affiliated to the genus rhodococcus were able to desulfonate arylsulfonates in wheat rhizospheres from the broadbalk long-term field wheat experiment, which includes plots treated with inorganic fertilizer with and without sulfate, with farmyard manure, and unfertilized plots. direct isolation of desulfonating rhizobacteria yielded rhodococcus strains which grew w ... | 2009 | 19120463 |
| [phenol degradation by rhodococcus opacus strain 1g]. | during cultivation in a liquid medium, the bacterium rhodococcus opacus 1g was capable of growing on phenol at a concentration of up to 0.75 g/l. immobilization of rhodococcus opacus 1g had a positive effect on cell growth in the presence of phenol at high concentrations. the substrate at concentrations of 1.0 and 1.5 g/l was completely utilized over 24 and 48 h, respectively. the key enzymes of phenol degradation (two pyrocatechol 1,2-dioxygenases and muconate cycloisomerase) were isolated. one ... | 2009 | 19235509 |
| [phenanthrene and anthracene degradation by microorganisms of the genus rhodococcus]. | the cells of rhodococcus opacus 412 and r. rhodnii 135 were adapted to phenanthrene and anthracene on a solid mineral medium. preliminary adaptation of the strains accelerated the metabolism of polyaromatic hydrocarbons and provided for the ability of microorganisms to grow on pheanthrene as a sole carbon and energy source in a liquid mineral medium. it was shown that phenanthrene was mineralized by the strains through 7,8-benzocoumarin, 1-hydroxy-2-naphthoaldehyde, 1-hydroxy-2-naphthoic acid, s ... | 2009 | 19382706 |
| effect of cell-surface hydrophobicity on bacterial conversion of water-immiscible chemicals in two-liquid-phase culture systems. | the effect of bacterial cell-surface hydrophobicity on the bioconversion of water-immiscible chemicals in an aqueous-organic (a/o) two-liquid-phase culture system was investigated. escherichia coli jm109 and rhodococcus opacus b-4 were used as hydrophilic and hydrophobic whole-cell catalysts, respectively. hydroxylation reactions of monoaromatics, including toluene (log p(ow)=2.9), ethylbenzene (3.1), n-propylbenzene (3.4), and sec-butylbenzene (3.7), were employed as model conversions. when the ... | 2009 | 19619857 |
| physiological and morphological responses of the soil bacterium rhodococcus opacus strain pd630 to water stress. | rhodococcus opacus pd630 was investigated for physiological and morphological changes under water stress challenge. gluconate- and hexadecane-grown cells were extremely resistant to these conditions, and survival accounted for up to 300 and 400 days; respectively, when they were subjected to slow air-drying. results of this study suggest that strain pd630 has specific mechanisms to withstand water stress. water-stressed cells were sensitive to the application of ethanol, high temperatures and ox ... | 2004 | 19712366 |
| degradation of selected (bio-)surfactants by bacterial cultures monitored by calorimetric methods. | the subjects of the article are investigations concerning the ability of both rhodococcus opacus 1cp and mixed bacterial cultures to use selected surfactants as sole carbon and energy source. in a comparative manner the biosurfactants rhamnolipid, sophorolipid and trehalose tetraester, and the synthetic surfactant tween 80 were examined. particular emphasis was put on a combinatorial approach to determine quantitatively the degree of surfactant degradation by applying calorimetry, thermodynamic ... | 2010 | 19714474 |
| enzymatic and genetic profiles in environmental strains grown on polycyclic aromatic hydrocarbons. | the possible generation of oxidative stress induced by aromatic hydrocarbon degradation suggests that ancillary enzyme activities could facilitate the utilization of polycyclic aromatic hydrocarbons as sole carbon source. to investigate the metabolic profiles of low molecular weight polycyclic aromatic hydrocarbon-degrading strains of sphingobium chlorophenolicum, rhodococcus aetherovorans, rhodococcus opacus and mycobacterium smegmatis, the determination of the activity of putative detoxifying ... | 2007 | 17109059 |
| utilization of hydrophobic bacterium rhodococcus opacus b-4 as whole-cell catalyst in anhydrous organic solvents. | rhodococcus opacus strain b-4, which has recently been isolated as an organic solvent-tolerant bacterium, has a high hydrophobicity and exhibits a high affinity for hydrocarbons. this bacterium was able to survive for at least 5 days in organic solvents, including n-tetradecane, oleyl alcohol, and bis(2-ethylhexyl) phthalate (behp), which contained water less than 1% (w/v). the biocatalytic ability of r. opacus b-4 was demonstrated in the essentially nonaqueous behp using indigo production from ... | 2007 | 17123076 |
| stya1 and stya2b from rhodococcus opacus 1cp: a multifunctional styrene monooxygenase system. | two-component flavoprotein monooxygenases are emerging biocatalysts that generally consist of a monooxygenase and a reductase component. here we show that rhodococcus opacus 1cp encodes a multifunctional enantioselective flavoprotein monooxygenase system composed of a single styrene monooxygenase (smo) (stya1) and another styrene monooxygenase fused to an nadh-flavin oxidoreductase (stya2b). stya1 and stya2b convert styrene and chemical analogues to the corresponding epoxides at the expense of f ... | 2010 | 20675468 |
| functional characterization and stability improvement of a 'thermophilic-like' ene-reductase from rhodococcus opacus 1cp. | ene-reductases (ers) are widely applied for the asymmetric synthesis of relevant industrial chemicals. a novel er oyero2 was found within a set of 14 putative old yellow enzymes (oyes) obtained by genome mining of the actinobacterium rhodococcus opacus 1cp. multiple sequence alignment suggested that the enzyme belongs to the group of 'thermophilic-like' oyes. oyero2 was produced in escherichia coli and biochemically characterized. the enzyme is strongly nadph dependent and uses non-covalently bo ... | 2015 | 26483784 |
| effect of alkyl hydroxybenzenes on the properties of dioxygenases. | the aim of the present work was to investigate the influence of alkylhydroxybenzenes (ahbs) and tyrosol, which belong to cell differentiation factors d(1) group of autoregulators on properties of biodegradation enzymes, catechol 1,2-dioxygenase (cat 1,2-do) and methylcatechol 1,2-dioxygenase (mcat 1,2-do) of rhodococcus opacus 6a. ahbs were found to have a greater effect on mcat 1,2-do than on cat 1,2-do. it was expressed by more pronounced changes in the activity of mcat 1,2-do with unsubstitut ... | 2010 | 20972919 |
| comparative transcriptomics elucidates adaptive phenol tolerance and utilization in lipid-accumulating rhodococcus opacus pd630. | lignin-derived (e.g. phenolic) compounds can compromise the bioconversion of lignocellulosic biomass to fuels and chemicals due to their toxicity and recalcitrance. the lipid-accumulating bacterium rhodococcus opacus pd630 has recently emerged as a promising microbial host for lignocellulose conversion to value-added products due to its natural ability to tolerate and utilize phenolics. to gain a better understanding of its phenolic tolerance and utilization mechanisms, we adaptively evolved r. ... | 2016 | 26837573 |
| glycogenformation by rhodococcus species and the effect of inhibition of lipid biosynthesis on glycogen accumulation in rhodococcus opacus pd630. | members of the genus rhodococcus were investigated for their ability to produce glycogen during cultivation on gluconate or glucose. strains belonging to rhodococcus ruber, rhodococcus opacus, rhodococcus fascians, rhodococcus erythropolis and rhodococcus equi were able to produce glycogen up to 0.2–5.6% of cellular dry weight (cdw). the glycogen content varied from 0.8% to 3.2% of cdw in cells of r. opacus pd630, which is a well-known oleaginous bacterium, during the exponential growth phase, w ... | 2010 | 21069909 |
| homologous gene clusters of nicotine catabolism, including a new ?-amidase for a-ketoglutaramate, in species of three genera of gram-positive bacteria. | gram-positive soil bacteria arthrobacter nicotinovorans, nocardioides sp. js614 and rhodococcus opacus were shown to contain similarly organized clusters of homologous genes for nicotine catabolism. an uncharacterized gene of a predicted nitrilase within these gene clusters was cloned from a. nicotinovorans and biochemical data unexpectedly showed that the protein exhibited ?-amidase activity toward a-ketoglutaramate. structural modelling of the protein suggested the presence of the catalytic tr ... | 2011 | 21288482 |
| isolation and diversity of natural product biosynthetic genes of cultivable bacteria associated with marine sponge mycale sp. from the coast of fujian, china. | the marine sponge mycale sp., a potential source of natural bioactive products, is widely distributed along the coast of fujian, china. the cultivable bacterial community associated with mycale sp., the antibacterial activities, and the pks (polyketide synthase) and nrps (nonribosomal peptide synthetase) gene diversity of these bacteria were investigated. phylogenetic analysis of the 16s rrna gene showed that the 51 isolates from mycale sp. belonged to actinobacteria, bacteroidetes, gammaproteob ... | 2014 | 24693980 |
| crystallization and preliminary x-ray diffraction analysis of recombinant chlorocatechol 1,2-dioxygenase from pseudomonas putida. | chlorocatechol 1,2-dioxygenase from the gram-negative bacterium pseudomonas putida (pp 1,2-ccd) is considered to be an important biotechnological tool owing to its ability to process a broad spectrum of organic pollutants. in the current work, the crystallization, crystallographic characterization and phasing of the recombinant pp 1,2-ccd enzyme are described. reddish-brown crystals were obtained in the presence of polyethylene glycol and magnesium acetate by utilizing the vapour-diffusion techn ... | 2011 | 21505253 |
| hydrocarbon-utilizing microorganisms naturally associated with sawdust. | sawdust, one of the materials used as sorbent for removing spilled oil from polluted environments was naturally colonized by hydrocarbon-utilizing fungi, 1×10(5)-2×10(5) colony forming units (cfu) g(-1), depending on the hydrocarbon substrate. this sorbent was initially free of hydrocarbon-utilizing bacteria. incubating wet sawdust at 30°c resulted in gradually increasing the fungal counts to reach after 6months between 5×10(6) and 7×10(6)cfug(-1), and the appearance of hydrocarbon-utilizing bac ... | 2011 | 21507457 |
| construction of a biobrick™ compatible vector system for rhodococcus. | throughout the past decade, the field of synthetic biology has grown rapidly. by using assembly platforms such as biobricks™, scientists can quickly and easily build gene circuits or multi-step pathways. one limitation, however, is that most of these parts were designed and characterized with escherichia coli as the target chassis. as a consequence, there exists a lack of standardized and well characterized or biobrick™ compatible plasmid backbones that replicate in other potential non-model cha ... | 2017 | 28130036 |
| improved xenobiotic-degrading activity of rhodococcus opacus strain 1cp after dormancy. | the goals of the present work were as follows: to obtain the dormant forms of r. opacus 1cp; to study the phenotypic variability during their germination; to compare phenotypic variants during the growth on selective and elective media; and to reveal changes in the ability of the strain to destruct xenobiotics that had not been degradable before dormancy. it was shown that rhodococcus opacus 1cp (the strain degrading chlorinated phenols) became able to utilize a broader spectrum of xenobiotics a ... | 2011 | 21749252 |
| Comparative and functional genomics of Rhodococcus opacus PD630 for biofuels development. | The Actinomycetales bacteria Rhodococcus opacus PD630 and Rhodococcus jostii RHA1 bioconvert a diverse range of organic substrates through lipid biosynthesis into large quantities of energy-rich triacylglycerols (TAGs). To describe the genetic basis of the Rhodococcus oleaginous metabolism, we sequenced and performed comparative analysis of the 9.27 Mb R. opacus PD630 genome. Metabolic-reconstruction assigned 2017 enzymatic reactions to the 8632 R. opacus PD630 genes we identified. Of these, 261 ... | 2011 | 21931557 |
| production of α-ketoisocaproate via free-whole-cell biotransformation by rhodococcus opacus dsm 43250 with l-leucine as the substrate. | this work aims to produce α-ketoisocaproate (kic) from l-leucine via the free-whole-cell biotransformation of rhodococcus opacus dsm 43250. the effects of temperature, ph, substrate concentration, cell concentration, and rotating speed on kic production were examined. furthermore, the biotransformation conditions were optimized with response surface methodology (rsm). the optimal biotransformation conditions were as follows: temperature 43.7 °c, ph 8.4, l-leucine concentration 5.1g/l, cell conce ... | 2011 | 22112557 |
| biochemical features of the degradation of pollutants by rhodococcus as a basis for contaminated wastewater and soil cleanup. | rhodococcus bacteria are considered to be promising degraders of persistent pollutants and are the basis of biological preparations for contaminated wastewater and soil cleanup. biotechnological application of this group of bacteria is based on the peculiaraties of their metabolism. this review briefly discusses the following main points: i. growth of rhodococcus on various aromatic substrates, ii. chloro/methylcatechol transformation pathways, 3-chlorocatechol branch of the modified ortho-pathw ... | 2011 | 22168001 |
| Metabolic engineering of hydrophobic Rhodococcus opacus for biodesulfurization in oil-water biphasic reaction mixtures. | An organic solvent-tolerant bacterium, Rhodococcus opacus B-4, was metabolically engineered to remove sulfur from dibenzothiophene (DBT), a component of crude oil. The resulting recombinant strain ROD2-8 constitutively expressed the Rhodococcus erythropolis IGTS8 genes dszA, dszB, and dszC, encoding dibenzothiophene sulfone monooxygenase, 2-(2'-hydroxyphenyl) benzenesulfinate desulfinase, and dibenzothiophene monooxygenase, respectively, of the 4S pathway to avoid transcriptional inhibition by t ... | 2011 | 22099375 |
| characterization of gordonia sp. strain cc-naph129-6 capable of naphthalene degradation. | a naphthalene-degrading isolate able to utilize naphthalene as a sole carbon source was identified as gordonia sp. cc-naph129-6. here a detail characterization of the naphthalene catabolic genes present in this strain was conducted. in nar region four structural genes (naraa, narab, narb, narc), two regulatory genes (narr1, narr2), a rubredoxin encoding gene (rub1) and a gene (orf7) with unknown function were obtained. when compared with most of the members within naphthalene-degrading rhodococc ... | 2012 | 22240034 |