| mutants defective in a mucor circinelloides dicer-like gene are not compromised in sirna silencing but display developmental defects. | dicer proteins are ribonuclease iii enzymes that process double stranded rna precursors into small rnas categorized as small interfering rnas (sirnas) or micrornas (mirnas), which suppress gene expression through the rna silencing mechanism. we have isolated a dicer-like gene (dcl-1) of mucor circinelloides, the first gene of this family to be identified in zygomycetes. the dcl-1 mrna occurred in multiple forms, including the truncated molecules that result from premature polyadenylation. null d ... | 2007 | 17074518 |
| mucor circinelloides was identified by molecular methods as a cause of primary cutaneous zygomycosis. | a case of primary cutaneous zygomycosis caused by mucor circinelloides is described. histopathology showed typical hyphae along with chlamydospores. the isolate was identified by molecular and phenotypic methods. the utility of sequence analysis of the internal transcribed spacer region is highlighted; however, further studies are needed to assess species genetic heterogeneity. | 2007 | 17122018 |
| non-aug translation initiation of a fungal ring finger repressor involved in photocarotenogenesis. | the ring finger protein crga acts as a negative regulator of light-induced carotene biosynthesis in the fungus mucor circinelloides. sequence analysis of the crga coding region upstream of the first aug codon revealed the existence of an additional non-canonical ring finger domain at the most n-terminal end of the protein. the newly identified ring finger domain is required for crga to regulate photocarotenogenesis, as deduced from site-directed mutagenesis experiments. the role of both ring fin ... | 2007 | 17403679 |
| regio- and stereo-selective hydroxylation of abietic acid derivatives by mucor circinelloides and mortierella isabellina. | mucor circinelloides and mortierella isabellina hydroxylated dehydroabietic acid (deha). deha was converted regio- and stereo-selectively by whole cells of mr. circinelloides to give 2alpha-hydroxydehydroabietic acid in a 75% molar conversion yield (11 mm from 14.7 mm deha) after 72 h in the cultivation medium containing 3% (v/v) tween 80. with cells of ma. isabellina, under the same conditions, 20.5 mm (6.5 g l(-1)) 2-hydroxydehydroabietic acid (alpha/beta=81/19) was formed from 26.4 mm deha. | 2005 | 16215830 |
| central venous catheter-associated fungemia secondary to mucormycosis. | mucormycosis can cause fatal infections in immuno-compromised persons. rhinocerebral and pulmonary disease predominate. diagnosis is challenging, dissemination frequent, and mortality high. positive blood cultures are rare. we report the first case of central venous catheter-associated mucormycosis fungemia (mucor circinelloides). early diagnosis and multi-modal therapy led to clinical and microbiological cure. | 2005 | 16308234 |
| [effects of conditions for obtaining sporangiospores of the inoculum on the morphology and productivity of the fungus mucor circinelloides var. lusitanicus 12 m, a producer of gamma-linolenic acid]. | effects of lipid composition of sporangiospores of the fungus mucor circinelloides var. lusitanicus 12 m, obtained within diverse time frames using distinct nutritive media, on the morphology of the fungus in submerged cultures, the yield of the biomass, and its content of gamma-linolenic acid have been studied. the levels of base phospholipids and individual fractions of neutral lipids in sporangiospores were correlated with the character of their germination. the spores characterized by a high ... | 2008 | 18924414 |
| the crts gene of xanthophyllomyces dendrorhous encodes a novel cytochrome-p450 hydroxylase involved in the conversion of beta-carotene into astaxanthin and other xanthophylls. | the conversion of beta-carotene into xanthophylls is a subject of great scientific and industrial interest. we cloned the crts gene involved in astaxanthin biosynthesis from two astaxanthin producing strains of xanthophyllomyces dendrorhous: vkpm y2410, an astaxanthin overproducing strain, and the wild type atcc 24203. in both cases, the orf has a length of 3166 bp, including 17 introns, and codes for a protein of 62.6 kda with similarity to cytochrome-p450 hydroxylases. crts gene sequences from ... | 2006 | 16455271 |
| molecular identification of zygomycetes from culture and experimentally infected tissues. | mucormycosis is an emerging infection associated with a high mortality rate. identification of the causative agents remains difficult and time-consuming by standard mycological procedures. in this study, internal transcribed spacer (its) sequencing was validated as a reliable technique for identification of zygomycetes to the species level. furthermore, species identification directly from infected tissues was evaluated in experimentally infected mice. fifty-four zygomycetes strains belonging to ... | 2006 | 16455881 |
| transformation of mucor circinelloides with autoreplicative vectors containing homologous and heterologous ars elements and the dominant cbx(r) carboxine-resistance gene. | mucor circinelloides transformants prototrophic to leucine and resistant to carboxine (leu(+) cbx(r)) have been obtained by treatment of protoplasts with plasmid constructs containing homologous leua gene and adjacent autonomously replicating sequences (ars) element combined with the cbx(r)(carboxine-resistance) gene of ustilago maydis and ars sequences from this basidiomycete (plasmid pgg37) or from the 2 mu plasmid of saccharomyces cerevisiae (plasmid pgg43). the presence in the same plasmid m ... | 2006 | 16502289 |
| [five cases of zygomycosis.]. | combined histological and mycological study of tissue specimens established a proven diagnosis of cutaneous zygomycosis in four patients. all patients had been treated with wide spectrum antibiotics and one patient (liver transplantation) was in addition also treated with cyclosporine. all had acidosis and cutaneous breaks and four had also been treated with systemic corticosteroids. the infecting organisms were absidia corymbifera (n=2), rhizopus stolonifer (n=1) and mucor circinelloides (n=1). ... | 1999 | 18473594 |
| intracellular fate of hydrocarbons: possible existence of specific compartments for their biodegradation. | in previous work, purification procedures and zymogram analysis conducted with supernatants of crude extracts from aerobic mycelium of the yr-1 strain of mucor circinelloides isolated from petroleum-contaminated soils indicated the existence of only one soluble alcohol oxidase (sao) activity. in the present work enzymatic activity of alcohol oxidase (ao) was also detected in the mixed membrane fraction (mmf) of a high-speed centrifugation procedure after drastic ballistic cellular homogenization ... | 2005 | 15917600 |
| detection of nad+-dependent alcohol dehydrogenase activities in yr-1 strain of mucor circinelloides, a potential bioremediator of petroleum contaminated soils. | different soluble nad+-dependent alcohol dehydrogenase (adh) isozymes were detected in cell-free homogenates from aerobically grown mycelia of yr-1 strain of mucor circinelloides isolated from petroleum- contaminated soil samples. depending on the carbon source present in the growth media, multiple nad+-dependent adhs were detected when hexadecane or decane was used as the sole carbon source in the culture media. adh activities from aerobically or anaerobically grown mycelium or yeast cells, res ... | 2005 | 15917606 |
| fatty acid production characteristics of fungi with particular emphasis on gamma linolenic acid production. | the fatty acid production characteristics of fungi are described. these characteristics are the relationship between the oil content of the cell and the fatty acid content of the oil. for example, for polyunsaturated fatty acid (pufa) production by mucor hiemalis ipd 51, the oil content of the cell and the gla content of the oil are coupled. for fungal production of some pufa, synthesized after the rate-limiting step in the fatty acid anabolic chain, a maximum fatty acid production model was dev ... | 1993 | 18613085 |
| the diversity of eukaryotic microbiota in the traditional slovak sheep cheese--bryndza. | we investigated the occurrence and diversity of yeasts and filamentous fungi in bryndza an artisanal slovak soft spreadable cheese prepared from raw sheep milk or from a mixture of sheep and cow milk. samples collected during four months of the summer production period from two locations (northern and southern parts of central slovakia) contained 10(5)-10(7) (cfu) yeasts and about 10(2) (cfu) of mold per gram of wet weight. further characterization by conventional taxonomy and sequence compariso ... | 2008 | 18639949 |
| effect of myoinositol on sporangiospore-yeast transformation of mucor circinelloides tieghem cultivated in synthetic broth. | mucor species exhibit fungal dimorphism in controlled environments. in this work, we examined the effect of myoinositol supplementation on the growth and morphology of mucor circinelloides. using sporangiospores as inoculums, diverse morphologies were induced in synthetic broth incubated at ph 4.5, temp. 20 degrees c, ambient. the morphologies included thallic suptypes (holoblastic-, holothallic-, enterothallic conidia as well as vesicular conidial headgroups), which were determinate in growth, ... | 2005 | 18697728 |
| a single dicer gene is required for efficient gene silencing associated with two classes of small antisense rnas in mucor circinelloides. | rna silencing in the zygomycete mucor circinelloides exhibits uncommon features, such as induction by self-replicative sense transgenes and the accumulation of two size classes of antisense small interfering rnas (sirnas). to investigate whether this silencing phenomenon follows the rules of a canonical rna-silencing mechanism, we used hairpin rna (hprna)-producing constructs as silencing triggers and analyzed the efficiency and stability of silencing in different genetic backgrounds. we show he ... | 2009 | 19666782 |
| [moulds and yeasts in bottled water and soft drinks]. | some damaged cartons of soft drinks and carbonated water were analyzed to detect the microorganisms that caused the damage. the contaminants of sugar used in the production of one of the drinks were also studied. the methods of déak & beuchat and pitt & hocking were used for the identification of yeasts and moulds, respectively. the agents of the spoilage of soft drinks were debaryomyces hansenii, debaryomyces polymorphus, galactomyces geotrichum, metschnikowia pulcherrima, mucor circinelloides, ... | 2006 | 17037258 |
| synthesis of 3beta, 7alpha, 11alpha-trihydroxy-pregn-21-benzylidene-5-en-20-one derivatives and their cytotoxic activities. | a series of novel 3beta, 7alpha, 11alpha-trihydroxy-pregn-21-benzylidene-5-en-20-one derivatives were synthesized and characterized by nmr, hrms. the pregnenolone (1) was first biotransformed by mucor circinelloides var lusitanicus to 3beta, 7alpha, 11alpha-trihydroxy-pregn-5-en-20-one (3), then 3 was treated with various benzaldehydes to produce 3beta, 7alpha, 11alpha-trihydroxy-pregn-21-benzylidene-5-en-20-one derivatives. these derivatives showed remarkable activity against ec109 cells. the a ... | 2009 | 19864129 |
| biological control of peach fungal pathogens by commercial products and indigenous yeasts. | the potential use of the commercial biocontrol products serenade (bacillus subtilis qst-713) and trichodex (trichoderma harzianum rifai strain t39) to inhibit the postharvest pathogenic molds penicillium crustosum and mucor circinelloides was investigated. both products exhibited antagonistic activity in vitro against the pathogens, reducing their growth at different levels. in addition, epiphytic yeasts isolated from peaches were identified as candida maltosa, pichia fermentans, and pichia kluy ... | 2006 | 17066929 |
| [activity of nad-dependent isocitrate dehydrogenase, isocitrate lyase, and malate dehydrogenase in mucor circinelloides var. lusitanicus inmi under different modes of nitrogen supply]. | the growth and morphology as well as lipogenesis and activity of the enzymes of the tricarboxylic acid cycle and the glyoxylate cycle were studied in the fungus mucor circinelloides var. lusitanicus inmi grown at various concentrations of urea (nitrogen source) added to the medium in different modes. it was shown that the maximum lipid content in the biomass was observed at a low (0.5 g/l) concentration of the nitrogen source, whereas the highest content of gamma-linolenic acid in the lipids was ... | 2008 | 18825970 |
| [metabolic characteristics and lipid composition of yeastlike cells and mycelium of mucor circinelloides var. lusitanicus inmi grown at a high glucose content in the medium]. | it is shown that the fungus mucor circinelloides var. lusitanicus inmi grown under aerobic conditions in a medium with a high glucose concentration (20%) is capable of both yeastlike and mycelial growth. in the mycelium, the activity of nad-dependent isocitrate dehydrogenase was more than twice as high as in yeastlike cells, whereas the isocitrate lyase activity was lower. a number of significant differences were found in the lipid composition of the cells of two different morphological variants ... | 2008 | 18825971 |
| cloning of the rhizomucor miehei 3-hydroxy-3-methylglutaryl-coenzyme a reductase gene and its heterologous expression in mucor circinelloides. | in this study, the gene hmgr encoding the 3-hydroxy-3-methylglutaryl-coenzyme a reductase (hmg-coa reductase) was cloned and characterized in the zygomycete fungus rhizomucor miehei. the hmgr gene comprises a total of 3,585 bp including the coding sequence of a 1,058 amino acids length putative protein and five introns (137, 83, 59, 60 and 69 bp in length) dispersed in the whole coding region. southern hybridization analysis revealed that the gene is present only in one copy in the r. miehei gen ... | 2009 | 18853273 |
| differential expression of rho1gtpase and rho3gtpase during isotropic and polarized growth of mucor circinelloides. | evidence has been obtained that indicates the presence of small 22 kda gtp-binding rho proteins through adp-ribosylation by clostridium botulinum c3 exotoxin in mucor circinelloides. rho protein was detected at all stages of growth studied. during polarized growth, both under aerobic conditions and during the yeast-mycelia transition, the radiolabeling of the [32p]adp-ribosylated protein increased when tube formation occurred and decreased as the hyphae branched. however, when mucor grew isotrop ... | 2007 | 17496964 |
| [breakthrough pulmonary mucormycosis during voriconazole treatment after reduced-intensity cord blood transplantation for a patient with acute myeloid leukemia]. | a 59-year-old man was admitted to our hospital with a diagnosis of acute myeloid leukemia in september 2004. he developed invasive pulmonary aspergillosis (ipa) and candidiasis, which were improved by administration of micafungin and amphotericin b (amph-b). he received reduced-intensity unrelated cord-blood transplantation without induction chemotherapy. he developed grade iv graft-versus-host disease (gvhd) and the administration of steroids against gvhd was prolonged. voriconazole (vrcz) was ... | 2007 | 17571588 |
| malic enzyme: the controlling activity for lipid production? overexpression of malic enzyme in mucor circinelloides leads to a 2.5-fold increase in lipid accumulation. | malic enzyme (me; nadp(+)-dependent; ec 1 . 1 . 1 . 40) has been postulated to be the rate-limiting step for fatty acid biosynthesis in oleaginous fungi in which the extent of lipid accumulation is below the maximum possible. the genes encoding the isoform of me involved in fatty acid synthesis were identified in mucor circinelloides and mortierella alpina, two commercially useful oil-producing fungi, using degenerate primers. both showed high similarity with me genes from other micro-organisms. ... | 2007 | 17600047 |
| pka from mucor circinelloides: model to study the role of linker i in the interaction between r and c subunits. | protein kinase a from the fungus mucor circinelloides shows high affinity interaction between regulatory (r) and catalytic (c) subunits. its r subunit shows a differential presence of several acidic residues in linker i region, in the amino terminus. mutants r1, lacking the n-terminal region, and r2, lacking the acidic cluster, were used to analyze its effect on the interaction with the c subunit, assessed through inhibition of catalytic activity and camp activation of reconstituted holoenzyme. ... | 2007 | 17761146 |
| ddt degradation potential of cattle manure compost. | the purpose of this study was to investigate the ability of cattle manure compost (cmc) to degrade 1,1,1-trichloro-2,2-bis (4-chlorophenyl) ethane (ddt). ddt was degraded during composting and 1,1-dichloro-2,2-bis (4-chlorophenyl) ethane (ddd) was detected as a metabolic product. degradation of ddt at 60 degrees c was the most effective of all the stages of composting. fourteen strains of fungi were isolated and identified from cmc, and most of them were closely related to mucor circinelloides a ... | 2010 | 20494402 |
| a novel activating effect of the regulatory subunit of protein kinase a on catalytic subunit activity. | the strength of the interaction between the catalytic and regulatory subunits in protein kinase a differs among species. the linker region from regulatory subunits is non-conserved. to evaluate the participation of this region in the interaction with the catalytic subunit, we have assayed its effect on the enzymatic properties of the catalytic subunit. protein kinase a from three fungi, mucor rouxii, mucor circinelloides and saccharomyces cerevisiae have been chosen as models. the r-c interactio ... | 2008 | 18854166 |
| lycopene over-accumulation by disruption of the negative regulator gene crga in mucor circinelloides. | lycopene has become one of the most interesting antioxidant compounds, especially in relation to human health. this work describes a genetic strategy to modify the carotenoid biosynthesis pathway to develop a lycopene-overproducing strain. the crga gene, a negative regulator of carotenogenesis, was disrupted in the mucor circinelloides strain mu202, which lacks the lycopene cyclase activity and accumulates lycopene instead of beta-carotene. the resultant strain, mu224, demonstrated increased tra ... | 2008 | 18060399 |
| a ring-finger photocarotenogenic repressor involved in asexual sporulation in mucor circinelloides. | mucor circinelloides responds to blue light by activating the biosynthesis of carotenoids and bending its sporangiophores towards the light source. the crga protein product acts as a repressor of carotene biosynthesis, as its inactivation leads to the overaccumulation of carotenoids in both the dark and the light. we show here that asexual sporulation in mucor is also stimulated by light and that the crga gene is involved in sporulation, given that lack of crga function affects both carotenogene ... | 2008 | 18194338 |
| conversion of beta-carotene into astaxanthin: two separate enzymes or a bifunctional hydroxylase-ketolase protein? | abstract: astaxanthin is a xanthophyll of great interest in animal nutrition and human health. the market prospect in the nutraceutics industries for this health-protective molecule is very promising. astaxanthin is synthesized by several bacteria, algae and plants from beta-carotene by the sequential action of two enzymes: a beta-carotene, 3,3'-hydroxylase that introduces an hydroxyl group at the 3 (and 3') positions of each of the two beta-ionone rings of beta-carotene, and a beta-carotene ket ... | 2008 | 18289382 |
| 4-dihydrotrisporin-dehydrogenase, an enzyme of the sex hormone pathway of mucor mucedo: purification, cloning of the corresponding gene, and developmental expression. | the nadp-dependent 4-dihydrotrisporin-dehydrogenase is a (-) mating-type-specific enzyme in the pathway from beta-carotene to trisporic acid. this substance and its isomers and derivatives represent the general system of sexual communication in zygomycetes. the (-) mating type of mucor mucedo was stimulated by trisporic acid and the enzyme was purified by ion exchange and affinity chromatography. several peptides of the 26-kda protein, digested with trypsin, were sequenced by mass spectrometry. ... | 2009 | 18931040 |
| small gtp-binding proteins are associated with chitosomes and vesicles carrying glucose oxidase from mucor circinelloides. | fractions enriched with chitosomes and vesicles carrying glucose oxidase (gox) activity from the dimorphic zygomycete mucor circinelloides were obtained using two successive sucrose gradients, the first a linear-log and the second an isopycnic gradient. using an [alpha-(32)p]gtp-binding assay, we detected the association of small gtp-binding proteins (21 and 17 kda) with both types of vesicles. in addition, by adp-ribosylation with c3 exotoxin, and western blot analysis with specific antibodies, ... | 2008 | 18310030 |
| a ring-finger protein regulates carotenogenesis via proteolysis-independent ubiquitylation of a white collar-1-like activator. | protein ubiquitylation plays a major role in the regulation of cellular processes mainly through proteasome-dependent degradation, although it has become increasingly clear that it is also involved in other processes. in the fungus mucor circinelloides, blue light regulates carotene biosynthesis, with this response controlled by crga and mcwc-1c genes. crga shows characteristics of ubiquitin ligases and represses carotenogenesis in the dark, whereas mcwc-1c is a white collar 1-like gene required ... | 2008 | 18976280 |
| high reliability transformation of the basal fungus mucor circinelloides by electroporation. | molecular approaches to study the biology of the zygomycete mucor circinelloides depend mainly on the existence of a polyethylene glycol-based transformation method, which is one of the most efficient in zygomycete fungi. however, the poor reliability and low transformation rates of this method are major obstacles in the molecular study of a number of biological processes. this paper describes an easy and reliable method to transform m. circinelloides protoplasts by electroporation. a high-volta ... | 2011 | 21256886 |
| [diversity of facultatively anaerobic microscopic mycelial fungi in soils]. | the numbers of microscopic fungi isolated from soil samples after anaerobic incubation varied from tens to several hundreds of cfu per one gram of soil; a total of 30 species was found. this group is composed primarily of mitotic fungi of the ascomycete affinity belonging to the orders hypocreales (fusarium solani, f. oxysporum, fusarium sp., clonostachys grammicospora, c. rosea. acremonium sp., gliocladium penicilloides, trichoderma aureoviride, t. harzianum, t. polysporum, t. viride. t. koning ... | 2008 | 18365728 |
| fungemia and cutaneous zygomycosis due to mucor circinelloides in an intensive care unit patient: case report and review of literature. | mucor spp. are rarely pathogenic in healthy adults, but can cause fatal infections in patients with immuosuppression and diabetes mellitus. documented mucor fungemia is a very rare condition in the literature. we described a fungemia and cutaneous mucormycosis case due to mucor circinelloides in an 83-year-old woman with diabetes mellitus who developed acute left frontoparietal infarctus while hospitalized in a neurological intensive care unit. the diagnosis was made based on the growth of fungi ... | 2009 | 19305057 |
| transcriptional activation increases rna silencing efficiency and stability in the fungus mucor circinelloides. | initiation and maintenance of the rna silencing mechanism was investigated after transcriptional activation of the transgene carb in mucor circinelloides. light induced transcription of the reporter gene carb specifically increased the 21-nt sirna compared with the 25-nt sirna and rose the silencing maintenance from 48% up to 93% of the descendent colonies. in accordance, induced transcription of the gene carb through disruption of the gene crga increased the initial silencing frequency up to 30 ... | 2009 | 19375461 |
| spectrum of zygomycete species identified in clinically significant specimens in the united states. | several members of the order mucorales (subphylum mucoromycotina) are important agents of severe human infections. the identification of these fungi by using standard mycologic methods is often difficult and time consuming. frequently, the etiological agent in clinical cases is reported either as a mucor sp., which is not the most frequent genus of zygomycetes, or only as a member of the mucorales. for this reason, the actual spectrum of species of zygomycetes and their incidences in the clinica ... | 2009 | 19386856 |
| a subunit of protein kinase a regulates growth and differentiation in the fungus mucor circinelloides. | the cyclic amp (camp)-dependent protein kinase a (pka) signaling pathway plays a role in regulating development, growth, and virulence in a number of fungi. to determine whether pka plays a similar function in zygomycete fungi, a mutant of mucor circinelloides was generated that lacks pkar1, one of the regulatory subunits of pka. the mutant showed a reduction in growth and alterations in germination rates, cell volume, germ tube length, and asexual sporulation. the lack of pkar1 gene resulted in ... | 2009 | 19411621 |
| intracellular distribution of fatty alcohol oxidase activity in mucor circinelloides yr-1 isolated from petroleum contaminated soils. | in previous studies, mucor circinelloides yr-1 isolated from petroleum-contaminated soils grown in decane as sole carbon source, showed fatty alcohol oxidase (fao) activities in either particulate or soluble fractions from a cell-free extract. one is associated to internal membranes (mfao) and the other one is soluble (sfao). both activities appear to be located in the cells in specific compartments other than peroxisomes. results suggested that mfao could be located on the inner face of the mem ... | 2009 | 19642009 |
| a new beta-glucosidase gene from the zygomycete fungus rhizomucor miehei. | in this study, a beta-glucosidase coding gene (bgl) of the zygomycete fungus rhizomucor miehei has been cloned and characterized. the gene comprises a total of 2,826 bp including the coding sequence of a 717 amino acids length putative protein and 10 introns dispersed in the whole coding region. the putative n-and c-terminal catalytic domains (aa 68 to aa 274 and aa 358-601, respectively) were identified; the two domains are connected with a 84-amino-acids linker. the catalytic region showed an ... | 2010 | 19813101 |
| molecular characterization of mucor circinelloides and rhizopus stolonifer strains isolated from some saudi fruits. | thirteen strains of mucor circinelloides and nine strains of rhizopus stolonifer were collected from different saudi fruits. also, two strains of m. circinelloides and one strain of r. stolonifer isolated from soil were used in this study to analyze the genetic diversity among these 25 strains. the dendrogram constructed from random amplified polymorphic dna-polymerase chain reaction results using two primers (v6 and m13) showed no correlation between clustering system and sources of isolation f ... | 2010 | 19877768 |
| first report of mucor circinelloides occurring on yellow catfish (pelteobagrus fulvidraco) from china. | infected yellow catfish (pelteobagrus fulvidraco) were sent from niushan lake fishery, hubei province, china, to our laboratory for diagnosis. macroscopic daffodil yellow mold was observed on the heads and fins of the fish and one mucor species was isolated. based on the morphological and molecular analysis, the species was identified as mucor circinelloides. its optimum growth temperature was 30 degrees c and it could not grow at 40 degrees c. the infectivity results showed wound infection coul ... | 2010 | 19930461 |
| anti-inflammatory drugs selectively target sporangium development in mucor. | it is known that acetylsalicylic acid, an anti-inflammatory and anti-mitochondrial drug, targets structure development and functions of yeasts depending on elevated levels of mitochondrial activity. using antibody probes, we previously reported that sporangia of mucor circinelloides also contain increased mitochondrial activity, yielding high levels of 3-hydroxyoxylipins. this was, however, not found in mortierella alpina (subgenus mortierella). in this study we report that acetylsalicylic acid ... | 2009 | 20029531 |
| genotypic analysis of mucor from the platypus in australia. | mucor amphibiorum is the only pathogen known to cause significant morbidity and mortality in the free-living platypus (ornithorhynchus anatinus) in tasmania. infection has also been reported in free-ranging cane toads (bufo marinus) and green tree frogs (litoria caerulea) from mainland australia but has not been confirmed in platypuses from the mainland. to date, there has been little genotyping specifically conducted on m. amphibiorum. a collection of 21 mucor isolates representing isolates fro ... | 2010 | 20090018 |
| spores of mucor ramosissimus, mucor plumbeus and mucor circinelloides and their ability to activate human complement system in vitro. | complement activation by spores of mucor ramosissimus, mucor plumbeus and mucor circinelloides was studied using absorbed human serum in the presence or absence of chelators (egta or edta). we found that the spore caused full complement activation when incubated with egta-mg2+ or without chelators, indicating that the alternative pathway is mainly responsible for this response. in order to compare activation profiles from each species, elisas for c3 and c4 fragments, mannan binding lectin (mbl), ... | 2010 | 20141371 |
| molecular detection and identification of zygomycetes species from paraffin-embedded tissues in a murine model of disseminated zygomycosis: a collaborative european society of clinical microbiology and infectious diseases (escmid) fungal infection study group (efisg) evaluation. | the present study was performed to assess the interlaboratory reproducibility of the molecular detection and identification of species of zygomycetes from formalin-fixed paraffin-embedded kidney and brain tissues obtained from experimentally infected mice. animals were infected with one of five species (rhizopus oryzae, rhizopus microsporus, lichtheimia corymbifera, rhizomucor pusillus, and mucor circinelloides). samples with 1, 10, or 30 slide cuts of the tissues were prepared from each paraffi ... | 2010 | 20375233 |
| endogenous short rnas generated by dicer 2 and rna-dependent rna polymerase 1 regulate mrnas in the basal fungus mucor circinelloides. | endogenous short rnas (esrnas) play diverse roles in eukaryotes and usually are produced from double-stranded rna (dsrna) by dicer. esrnas are grouped into different classes based on biogenesis and function but not all classes are present in all three eukaryotic kingdoms. the esrna register of fungi is poorly described compared to other eukaryotes and it is not clear what esrna classes are present in this kingdom and whether they regulate the expression of protein coding genes. however, evidence ... | 2010 | 20427422 |
| photobiology in the zygomycota: multiple photoreceptor genes for complex responses to light. | light is an environmental signal that modulates many aspects of the biology of zygomycete fungi. light regulation has been investigated in the zygomycetes phycomyces blakesleeanus, mucor circinelloides and pilobolus crystallinus. examples of light regulation include the phototropism of the fruiting bodies, the regulation of the development of reproductive structures, and the activation of the biosynthesis of β-carotene. in fungi blue light is perceived by proteins homologous to wc-1, a neurospor ... | 2010 | 20466063 |
| analysis of glycerol dehydrogenase activities present in mucor circinelloides yr-1. | two inducible nadp(+)-dependent glycerol dehydrogenase (glcdh) activities were identified in mucor circinelloides strain yr-1. one of these, denoted iglcdh2, was specifically induced by n-decanol when it was used as sole carbon source in the culture medium, and the second, denoted iglcdh1, was induced by alcohols and aliphatic or aromatic hydrocarbons when glycerol was used as the only substrate. iglcdh2 was found to have a much broader substrate specificity than iglcdh1, with a low activity as ... | 2010 | 20512634 |
| fungal photobiology: a synopsis. | fungi respond and adapt to many environmental signals including light. the photobiology of fungi has been extensively investigated, but in recent years the identification of the first fungal photoreceptor, wc-1 in the ascomycete neurospora crassa, and the discovery that similar photoreceptors are required for photoreception in other ascomycete, basidiomycete and zygomycete fungi has allowed the molecular characterization of light reception and the early steps of signal transduction in a number o ... | 2011 | 22679584 |
| fungal outbreak in a show cave. | castañar de ibor cave (spain) was discovered in 1967 and declared a natural monument in 1997. in 2003 the cave was opened to public visits. despite of extensive control, on 26 august 2008 the cave walls and sediments appeared colonized by long, white fungal mycelia. this event was the result of an accidental input of detritus on the afternoon of 24 august 2008. we report here a fungal outbreak initiated by mucor circinelloides and fusarium solani and the methods used to control it. | 2010 | 20553941 |
| transcription of the three hmg-coa reductase genes of mucor circinelloides. | precursors of sterols, carotenoids, the prenyl groups of several proteins and other terpenoid compounds are synthesised via the acetate-mevalonate pathway. one of the key enzyme of this pathway is the 3-hydroxy-3-methylglutaryl-coa (hmg-coa) reductase, which catalyses the conversion of hmg-coa to mevalonate. hmg-coa reductase therefore affects many biological processes, such as morphogenesis, synthesis of different metabolites or adaptation to environmental changes. in this study, transcription ... | 2014 | 24731286 |
| cloning and expression of an active aspartic proteinase from mucor circinelloides in pichia pastoris. | extracellular aspartic proteinase (mcap) produced by mucor circinelloides in solid state fermentations has been shown to possess milk clotting activity and represents a potential replacement for bovine chymosin in cheese manufacturing. despite its prospects in the dairy industry, the molecular characteristics of this enzyme remain unknown. this work focuses on mcap cloning and optimization of heterologous expression in pichia pastoris, and characterization of the enzyme. | 2013 | 24206750 |
| evaluation of aerial microbial pollutants in al-haram al-nabawi during pilgrimage of 2013. | al-madinah al-munawwarah is the second holiest site in islam. the possibility of new emerging microbes is valid due to the increased number of pilgrims. the objectives of the current study were to estimate the numbers of fungi and bacteria inside and outside al-haram al-nabawi and to find whether new bacterial and fungal species have emerged compared to previous studies. air samples were collected twice a day from 12 spots and four directions during the pilgrim year of 2013 for four consecutive ... | 2015 | 28053593 |
| effect of dimorphic regulation on heterologous glucose oxidase production by mucor circinelloides. | the production of aspergillus niger glucose oxidase (gox) and native amylase by the recombinant m. circinelloides kfa199 strain under conditions of dimorphic growth was investigated. the recombinant kfa199 strain was compared to its parental atcc 1216b strain and a wild-type cbs 232.29 strain under similar morphology-controlled conditions. cultivation in vogel's medium supplemented with ergosterol/tween-80 and sparged with nitrogen gas was most suitable for yeast-like biomass production under an ... | 2010 | 20568082 |
| production, purification, and characterization of polygalacturonase from mucor circinelloides itcc 6025. | mucor circinelloides produced an extracellular polygalacturonase enzyme, the production of which was enhanced when various production parameters were optimized. maximum polygalacturonase (pgase) activity was obtained in 48 h at 30°c and ph 4.0 with pectin methyl ester (1% w/v) as carbon source and a combination of casein hydrolysate (0.1% w/v) and yeast extract (0.1% w/v) as nitrogen source. the enzyme was purified to homogeneity (13.3-fold) by sephacryl s-100 gel-filtration chromatography. its ... | 2010 | 21048861 |
| effect of the sesterterpene-type metabolites, ophiobolins a and b, on zygomycetes fungi. | ophiobolins are sesterterpene-type phytotoxins produced by fungi belonging mainly to the genus bipolaris. in this study, the antifungal effect of ophiobolins a and b on different zygomycetes has been examined. depending on the zygomycete tested, mic values of 3.175-50 μg ml(-1) were found for ophiobolin a and 25-50 μg ml(-1) for ophiobolin b. ophiobolin a inhibited sporangiospore germination of mucor circinelloides and caused morphological changes; the fungus formed degenerated, thick or swollen ... | 2010 | 21054501 |
| integration of a bacterial β-carotene ketolase gene into the mucor circinelloides genome by the agrobacterium tumefaciens-mediated transformation method. | plasmids introduced in mucor circinelloides (and most transformable mucorales) tend to replicate autonomously, and hardly ever integrate in the genome. this is critical if we want to express exogenous genes, because plasmids are easily lost during vegetative growth, and the ratio of plasmid molecules/nuclei is invariably low. linearized molecules of dna have been used to get their genomic integration but the transformation efficiency drops extremely. we have developed and highly optimized an eff ... | 2012 | 22711121 |
| the effects of palm oil breakdown products on lipid turnover and morphology of fungi. | the oleaginous fungi cryptococcus curvatus and mucor circinelloides were used to determine the effect of palm oil breakdown products, measured as polymerized triglycerides (ptgs), on lipid turnover and on fungal growth and morphology. in m. circinelloides, we found after 7 days of growth, a decrease in biomass and in lipid utilization and accumulation at increased ptg levels, both at low and neutral ph. in c. curvatus, there was also a decrease in lipid utilization and biomass production at incr ... | 2010 | 21076478 |
| structure, function, and phylogeny of the mating locus in the rhizopus oryzae complex. | the rhizopus oryzae species complex is a group of zygomycete fungi that are common, cosmopolitan saprotrophs. some strains are used beneficially for production of asian fermented foods but they can also act as opportunistic human pathogens. although r. oryzae reportedly has a heterothallic (+/-) mating system, most strains have not been observed to undergo sexual reproduction and the genetic structure of its mating locus has not been characterized. here we report on the mating behavior and genet ... | 2010 | 21151560 |
| mucor circinelloides as a cause of invasive maxillofacial zygomycosis: an emerging dimorphic pathogen with reduced susceptibility to posaconazole. | a case of maxillofacial zygomycosis caused by mucor circinelloides, identified by phenotypic and molecular methods and treated successfully with liposomal amphotericin b (ambisome) and surgical debridement, is described. the isolate was resistant to posaconazole. this report underscores the importance of prior susceptibility testing of zygomycetes to guide therapy with the most effective antifungal agent for an improved prognosis. | 2009 | 19171681 |
| activity of posaconazole and other antifungal agents against mucorales strains identified by sequencing of internal transcribed spacers. | the antifungal susceptibility profiles of 77 clinical strains of mucorales species, identified by internal transcribed spacer sequencing, were analyzed. mics obtained at 24 and 48 h were compared. amphotericin b was the most active agent against all isolates, except for cunninghamella and apophysomyces isolates. posaconazole also showed good activity for all species but cunninghamella bertholletiae. voriconazole had no activity against any of the fungi tested. terbinafine showed good activity, e ... | 2009 | 19171801 |
| a case of rhinoorbital mucormycosis in a leukemic patient with a literature review from turkey. | mucormycosis (zygomycosis) is a rare, invasive, opportunistic fungal infection of the paranasal sinuses, caused by a fungus of the order mucorales. we report a case of rhinoorbital mucormycosis caused by rhizopus oryzae in an acute lymphoblastic leukemia patient and review the 79 mucormycosis cases reported in the last decade from turkey. in our case, the diagnosis was made with endoscopic appearance, computerized tomography of the paranasal sinuses, and culture of the surgical materials. follow ... | 2011 | 21761152 |
| sporangiospore size dimorphism is linked to virulence of mucor circinelloides. | mucor circinelloides is a zygomycete fungus and an emerging opportunistic pathogen in immunocompromised patients, especially transplant recipients and in some cases otherwise healthy individuals. we have discovered a novel example of size dimorphism linked to virulence. m. circinelloides is a heterothallic fungus: (+) sex allele encodes sexp and (-) sex allele sexm, both of which are hmg domain protein sex determinants. m. circinelloides f. lusitanicus (mcl) (-) mating type isolates produce larg ... | 2011 | 21698218 |
| identification of a critical determinant that enables efficient fatty acid synthesis in oleaginous fungi. | microorganisms are valuable resources for lipid production. what makes one microbe but not the other able to efficiently synthesize and accumulate lipids is poorly understood. in the present study, global gene expression prior to and after the onset of lipogenesis was determined by transcriptomics using the oleaginous fungus mortierella alpina as a model system. a core of 23 lipogenesis associated genes was identified and their expression patterns shared a high similarity among oleaginous microb ... | 2015 | 26059272 |
| occurrence of fungal species and mycotoxins from decayed sugarcane (saccharrum officinarum) in egypt. | seventy-three fungal species belonging to forty-three genera were isolated from 40 samples of saccharrum officinarum (collected from naage-hamadi canal in qena governorate, egypt). aspergillus, trichoderma, mucor and pythium were the most common genera on the two isolation media. the dominant species of aspergillus were a. niger, a. flavus, a. ustus, a. terreus and a. wentii. some species were dominant on 40 g/l sucrose such as aspergillus niger, a. flavus, emericella nidulans, trichoderma virid ... | 2005 | 24049478 |
| Mould routine identification in the clinical laboratory by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. | MALDI-TOF MS recently emerged as a valuable identification tool for bacteria and yeasts and revolutionized the daily clinical laboratory routine. But it has not been established for routine mould identification. This study aimed to validate a standardized procedure for MALDI-TOF MS-based mould identification in clinical laboratory. | 2011 | 22194834 |
| physico-chemical characteristics and functional properties of chitin and chitosan produced by mucor circinelloides using yam bean as substrate. | microbiological processes were used for chitin and chitosan production by mucor circinelloides (ucp 050) grown in yam bean (pachyrhizus erosus l. urban) medium. the polysaccharides were extracted by alkali-acid treatment and structural investigations by x-ray diffraction, fourier transform ir analysis, viscosity and thermal analysis by tg, dtg, and dta were done. the highest biomass yield (20.7 g/l) was obtained at 96 hours. the highest levels of chitosan (64 mg/g) and chitin (500 mg/g) were pro ... | 2011 | 21862956 |
| analysis of the effect of soil saprophytic fungi on the eggs of baylisascaris procyonis. | baylisascaris procyonis is a soil-transmitted helminth mainly found in raccoons (procyon lotor) which can also affect other domestic and sylvatic animals, as well as humans, when the eggs released in the feces of parasitized raccoons are accidentally ingested. three assays have been conducted to assess the effect of three saprophytic fungi, mucor circinelloides, paecilomyces lilacinus, and verticillium sp., on the eggs of b. procyonis. firstly, their ovicidal effect was in vitro ascertained by p ... | 2015 | 25828813 |
| Alternative routes of acetyl-CoA synthesis identified by comparative genomic analysis: involvement in the lipid production of oleaginous yeast and fungi. | For a bio-based economy, microbial lipids offer a potential solution as alternative feedstocks in the oleochemical industry. The existing genome data for the promising strains, oleaginous yeasts and fungi, allowed us to investigate candidate orthologous sequences that participate in their oleaginicity. Comparative genome analysis of the non-oleaginous (Saccharomyces cerevisiae, Candida albicans and Ashbya gossypii) and oleaginous strains (Yarrowia lipolytica, Rhizopus oryzae, Aspergillus oryzae ... | 2012 | 22016567 |
| molecular and morphological identification of fungal species isolated from bealmijang meju. | bealmijang is a short-term aged paste made from meju, which is a brick of fermented soybeans and other ingredients. different types of bealmijang are available depending on the geographic region or ingredients used. however, no study has clarified the microbial diversity of these types. we identified 17 and 14 fungal species from black soybean meju (bsm) and buckwheat meju (bwm), respectively, on the basis of morphology, culture characteristics, and internal transcribed spacer and beta-tubulin g ... | 2011 | 22210613 |
| rapamycin exerts antifungal activity in vitro and in vivo against mucor circinelloides via fkbp12-dependent inhibition of tor. | the zygomycete mucor circinelloides is an opportunistic fungal pathogen that commonly infects patients with malignancies, diabetes mellitus, and solid organ transplants. despite the widespread use of antifungal therapy in the management of zygomycosis, the incidence of infections continues to rise among immunocompromised individuals. here, we establish that the targets and mechanism of antifungal action of the immunosuppressant rapamycin in m. circinelloides is mediated via conserved complexes w ... | 2011 | 22210828 |
| disseminated fungal infection in two species of captive sharks. | in this report, two cases of systemic mycosis in captive sharks are characterized. these cases were progressive and ultimately culminated in terminal disease. paecilomyces lilacinus, an uncommon pathogen in human and veterinary medicine, was associated with areas of necrosis in the liver, heart, and gill in a great hammerhead shark (sphyrna mokarran). fungal growth was observed from samples of kidney, spleen, spinal fluid, and coelomic cavity swabs. dual fungal infection by exophiala pisciphila ... | 2011 | 22204064 |
| A new antifungal coumarin from Clausena excavata. | A new ?-lactone coumarin, named as excavarin-A, showing antifungal activity was isolated from the leaves of Clausena excavata by bioassay guided fractionation method. The structure was elucidated by spectroscopic data analysis and identified as 7((2E)-4(4,5-dihydro-3-methylene-2-oxo-5-furanyl)-3-methylbut-2-enyloxy) coumarin. Minimum inhibitory concentration (MIC) was determined against fifteen fungal strains pathogenic against plants and human. The least MIC was recorded against the human patho ... | 2012 | 22088496 |
| Functional analysis of the Phycomyces carRA gene encoding the enzymes phytoene synthase and lycopene cyclase. | Phycomyces carRA gene encodes a protein with two domains. Domain R is characterized by red carR mutants that accumulate lycopene. Domain A is characterized by white carA mutants that do not accumulate significant amounts of carotenoids. The carRA-encoded protein was identified as the lycopene cyclase and phytoene synthase enzyme by sequence homology with other proteins. However, no direct data showing the function of this protein have been reported so far. Different Mucor circinelloides mutants ... | 2011 | 21858003 |
| Rice straw-decomposing fungi and their cellulolytic and xylanolytic enzymes. | Filamentous fungi colonizing rice straw were collected from 11 different sites in Korea and were identified based on characterization of their morphology and molecular properties. The fungi were divided into 25 species belonging to 16 genera, including 14 ascomycetes, one zygomycete, and one basidiomycete. Fungal cellulolytic and xylanolytic enzymes were assessed through a two-step process, wherein highly active cellulase- and/or hemicellulaseproducing fungi were selected in a first screening st ... | 2011 | 22210620 |
| Antifungal susceptibility and phylogeny of opportunistic members of the order mucorales. | The in vitro susceptibilities of 66 molecularly identified strains of the Mucorales to eight antifungals (amphotericin B, terbinafine, itraconazole, posaconazole, voriconazole, caspofungin, micafungin, and 5-fluorocytosine) were tested. Molecular phylogeny was reconstructed based on the nuclear ribosomal large subunit to reveal taxon-specific susceptibility profiles. The impressive phylogenetic diversity of the Mucorales was reflected in susceptibilities differing at family, genus, and species l ... | 2012 | 22075600 |
| two distinct rna-dependent rna polymerases are required for initiation and amplification of rna silencing in the basal fungus mucor circinelloides. | rna-dependent rna polymerases (rdrps) play key roles in the rna silencing pathway in a number of organisms. they have been involved in the production of double-stranded rna (dsrna) molecules that initiate the silencing mechanism as well as in the amplification of the silencing signal. the roles of rdrps from fungi in these processes are poorly described compared with other eukaryotes. rna silencing in the zygomycete mucor circinelloides exhibits uncommon features, such as induction by self-repli ... | 2011 | 22141923 |
| value-added oil and animal feed production from corn-ethanol stillage using the oleaginous fungus mucor circinelloides. | this study highlights the potential of oleaginous fungus, mucor circinelloides in adsorbing/assimilating oil and nutrients in thin stillage (ts), and producing lipid and protein-rich fungal biomass. fungal cultivation on ts for 2days in a 6-l airlift bioreactor, resulted in a 92% increase in oil yield from ts, and 20g/l of fungal biomass (dry) with a lipid content of 46% (g of oil per 100g dry biomass). reduction in suspended solids and soluble chemical oxygen demand (scod) in ts were 95% and 89 ... | 2011 | 22237170 |
| [anaerobic growth ability and alcohol fermentation activity of microscopic fungi]. | the method proposed in this study was used to isolate fungi grown under anaerobic conditions and to reveal distinctions in their abundance and species composition in different habitats. the ability of micromycetes of different taxa to grow under anaerobic conditions and ensure alcohol fermentation was determined for a representative sample (344 strains belonging to more than 60 species). the group of fungi growing under anaerobic conditions included species with high, moderate, and low fermentat ... | 2011 | 22808743 |
| coprophilous mucorales (ex zygomycota) from three areas in the semi-arid of pernambuco, brazil. | mucorales comprises fungi commonly isolated as saprobes from soil, dung, stored grains and plants. although these fungi have been studied in several countries, there are relatively a few reports of them in semi-arid areas. therefore, the aims of the present study were to assess and compare the mucorales communities in dung from different species and breeds of herbivores in the semi-arid of pernambuco, based on the frequency of occurrence and species richness of these fungi. samples of dung colle ... | 2016 | 27769884 |
| new insight into microbial diversity and functions in traditional vietnamese alcoholic fermentation. | the roles of microorganisms in traditional alcoholic fermentation are often assumed based on abundance in the starter and activity in pure culture. there is a serious lack of hard evidence on the behavior and activity of individual microbial species during the actual fermentation process. in this study, microbial succession and metabolite changes during 7days of traditional vietnamese alcoholic fermentation were monitored. special attention was devoted to starch degradation. in total, 22 microbi ... | 2016 | 27236224 |
| canthaxanthin production with modified mucor circinelloides strains. | canthaxanthin is a natural diketo derivative of β-carotene primarily used by the food and feed industries. mucor circinelloides is a β-carotene-accumulating zygomycete fungus and one of the model organisms to study the carotenoid biosynthesis in fungi. in this study, the β-carotene ketolase gene (crtw) of the marine bacterium paracoccus sp. n81106 fused with fungal promoter and terminator regions was integrated into the m. circinelloides genome to construct stable canthaxanthin-producing strains ... | 2013 | 23224586 |
| in vitro antioxidant and antifungal properties of essential oils obtained from aromatic herbs endemic to the southeast of spain. | the aim of this work was to determine (i) the antioxidant capacity of thymus moroderi, thymus piperella, santolina chamaecyparissus, and sideritis angustifolia essential oils (eos) by means of four different antioxidant tests (the 2,2'-diphenyl-1-picrylhydrazyl radical scavenging method, the ferrous ion-chelating ability assay, the ferric reducing antioxidant power test, and the thiobarbituric acid reactive species test) and (ii) the antifungal activity against molds and yeast of these eos by th ... | 2013 | 23834797 |
| molecular characterization and in vitro antifungal susceptibility of 80 clinical isolates of mucormycetes in delhi, india. | mucormycosis is a highly aggressive disease which is usually fatal in immunocompromised patients. the species of mucormycetes show significant differences in susceptibility to amphotericin b, azoles and terbinafine. the precise species level identification for this fungal group could be achieved by internal transcribed-spacer (its) region sequencing. herein, we present the largest series of antifungal susceptibility data of molecularly characterised isolates of mucormycetes reported so far from ... | 2014 | 25250768 |
| efficacy of a coating composed of chitosan from mucor circinelloides and carvacrol to control aspergillus flavus and the quality of cherry tomato fruits. | cherry tomato (lycopersicon esculentum mill) fruits are susceptible to contamination by aspergillus flavus, which may cause the development of fruit rot and significant postharvest losses. currently there are significant drawbacks for the use of synthetic fungicides to control pathogenic fungi in tomato fruits, and it has increased the interest in exploring new alternatives to control the occurrence of fungal infections in these fruits. this study evaluated the efficacy of chitosan (chi) from mu ... | 2015 | 26257717 |
| in vitro antifungal susceptibility of filamentous fungi causing rare infections: synergy testing of amphotericin b, posaconazole and anidulafungin in pairs. | mucormycetes (formerly known as zygomycetes of the order mucorales) and hyaline moulds such as those of the genus fusarium or paecilomyces are emerging as significant human pathogens. the aim of the study was to determine the in vitro antifungal susceptibility of these fungi to older and newer antifungals and to investigate the antifungal activity of amphotericin b, posaconazole and anidulafungin in dual combinations. | 2012 | 22535624 |
| expression of three isoprenoid biosynthesis genes and their effects on the carotenoid production of the zygomycete mucor circinelloides. | the zygomycete mucor circinelloides accumulates ß-carotene as the main carotenoid compound. in this study, the applicability of some early genes of the general isoprenoid pathway to improve the carotenoid production in this fungus was examined. the isopentenyl pyrophosphate isomerase gene (ipi) was cloned and used together with the genes encoding farnesyl pyrophosphate synthase (isoa) and geranylgeranyl pyrophosphate synthase (carg) in overexpression studies. transformation experiments showed th ... | 2011 | 21443966 |
| a new cultivation method for microbial oil production --- cell pelletization and lipid accumulation by mucor circinelloides. | abstract: introduction: the recent energy crisis has triggered significant attention on the microbial synthesis of lipids, which comprise the raw material for biodiesel production. microbial oil accumulation with filamentous fungi has great potential because filamentous fungi can form pellets during cell growth, and these aggregated cell pellets are much easier to harvest from cell broth solution than suspended individual cells. methods: this paper focuses on the cell pelletization process of th ... | 2011 | 21635739 |
| phosphorylation of calcineurin at a novel serine-proline rich region orchestrates hyphal growth and virulence in aspergillus fumigatus. | the fungus aspergillus fumigatus is a leading infectious killer in immunocompromised patients. calcineurin, a calmodulin (cam)-dependent protein phosphatase comprised of calcineurin a (cnaa) and calcineurin b (cnab) subunits, localizes at the hyphal tips and septa to direct a. fumigatus invasion and virulence. here we identified a novel serine-proline rich region (sprr) located between two conserved cnaa domains, the cnab-binding helix and the cam-binding domain, that is evolutionarily conserved ... | 2013 | 23990785 |
| effects of post-harvest treatment using chitosan from mucor circinelloides on fungal pathogenicity and quality of table grapes during storage. | the aim of this study was to extract chitosan (chi) from mucor circinelloides ucp 050 grown in a corn steep liquor (csl)-based medium under optimized conditions and to assess the efficacy of the obtained chi to inhibit the post-harvest pathogenic fungi aspergillus niger urm 5162 and rhizopus stolonifer urm 3482 in laboratory media and as a coating on table grapes (vitis labrusca l.). the effect of chi coating on some physical, physicochemical and sensory characteristics of the fruits during stor ... | 2014 | 25084665 |
| improvement of tea leaves fermentation through pectinases. | the pectinase enzymes isolated from aspergillus niger, byssochlamys fulva and mucor circinelloides were used for fermentation of tea leaves from camellia sinensis plant. the use of partially purified enzymes from aspergillus niger and mucor circinelloides resulted in significant (p < 0.001) increase in the phenolic compounds, hence, improvement in tea quality. maximum increase in phenolic compounds was found in tea leaves treated with partially purified polygalacturonase (pgase) from mucor circi ... | 2012 | 22982636 |
| antifungal and cytotoxic assessment of lapachol derivatives produced by fungal biotransformation. | in the screening for biological active compounds, the biotransformation processes catalyzed by filamentous fungi are useful because they can provide information about the possible appearance of toxic metabolites after oral administration and also generate new leads. in this paper, biotransformation of lapachol (1) by three fungal strains, mucor circinelloides nrrl3631, botrytis cinerea uca992 and botrytis cinerea 2100, has been investigated for the first time. lapachol (1) was biotransformed int ... | 2016 | 26996030 |
| the interaction of fungi with the environment orchestrated by rnai. | the fungal kingdom has been key in the investigation of the biogenesis and function of small rnas (srnas). the discovery of phenomena such as quelling in neurospora crassa represents pioneering work in the identification of the main elements of the rna interference (rnai) machinery. recent discoveries in the regulatory mechanisms in some yeast and filamentous fungi are helping us reach a deeper understanding of the transcriptional and post-transcriptional gene-silencing mechanisms involved in ge ... | 2016 | 26932186 |
| effect of the filamentous fungus mucor circinelloides on the development of eggs of the rumen fluke calicophoron daubneyi (paramphistomidae). | ruminants infected by paramphistomidae flukes shed eggs in the feces, which pass through different stages in the environment until the infective stages (metacercariae) are reached. the activity of the soil fungus mucor circinelloides on the development of eggs of the rumen fluke calicophoron daubneyi was presently tested with 3 probes, i.e., in petri plates, feces and an aqueous environment (tubes). the effect of the fungus was assessed by recording the numbers of undeveloped, non-viable and emb ... | 2017 | 28165868 |
| metagenomics analysis of microbial communities associated with a traditional rice wine starter culture (xaj-pitha) of assam, india. | this is the first report on the microbial diversity of xaj-pitha, a rice wine fermentation starter culture through a metagenomics approach involving illumine-based whole genome shotgun (wgs) sequencing method. metagenomic dna was extracted from rice wine starter culture concocted by ahom community of assam and analyzed using a miseq(®) system. a total of 2,78,231 contigs, with an average read length of 640.13 bp, were obtained. data obtained from the use of several taxonomic profiling tools were ... | 2016 | 28330225 |
| search for fungi-specific metabolites of four model drugs in postmortem blood as potential indicators of postmortem fungal metabolism. | fungi colonizing cadavers are capable of drug metabolism and may thus change the metabolite pattern or concentration of drugs in forensic postmortem samples. the purpose of this study was to check for the presence of such changes by searching fungi-specific metabolites of four model drugs (amitriptyline, metoprolol, mirtazapine, and zolpidem) in decomposed postmortem blood samples from 33 cases involving these drugs. after isolation and identification of fungal strains present in the samples, ea ... | 2016 | 27022860 |
| isolation of fungal pathogens to an edible mushroom, pleurotus eryngii, and development of specific its primers. | fungal pathogens have caused severe damage to the commercial production of pleurotus eryngii, the king oyster mushroom, by reducing production yield, causing deterioration of commercial value, and shortening shelf-life. four strains of pathogenic fungi, including trichoderma koningiopsis dc3, phomopsis sp. mp4, mucor circinelloides mp5, and cladosporium bruhnei mp6, were isolated from the bottle culture of diseased p. eryngii. a species-specific primer set was designed for each fungus from the i ... | 2013 | 24493949 |