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coyote as a final host for sarcocystis species of goats, sheep, cattle, elk, bison, and moose in montana.tissues (1 kg) from sheep, goats, cattle, moose, bison, or elk naturally infected with sarcocystis species were fed to one to four sarcocystis-free coyotes and the number of sporocysts in feces and intestines were counted. all 12 coyotes fed naturally infected tissues shed sarcocystis in feces, with a prepatent period of 9 to 15 days. the four coyotes fed infected beef had 15, 25, 113, and 201 million sporocysts in their feces and intestines. the coyotes fed elk, moose, or bison had 2.5, 15, and ...19806778270
serologic response of captive coyotes (canis latrans say) to canine parvovirus and accompanying profiles of canine coronavirus titers.fifty-five of 66 (83%) coyote pups from bitches vaccinated against canine parvovirus (cpv) were seropositive for cpv antibodies at birth. the cpv antibody titer in the pups declined with a half-life of 6.7 days until by the 8th week, only two of 41 (5%) pups were seropositive for cpv antibodies. at 8 wk, 41 of the pups were vaccinated against cpv (killed feline origin vaccine), but only one of 37 (3%) was positive for cpv antibodies at 11 wk. the 8-wk-old pups were either too young to respond to ...19846325726
rabies surveillance in the united states during 1994.in 1994, 48 states, the district of columbia, and puerto rico reported 8,224 cases of rabies in nonhuman animals and 6 cases in human beings to the centers for disease control and prevention. nearly 93% (7,632 cases) were wild animals, whereas 7% (592 cases) were domestic species. the total number of reported cases decreased 13.4% from that of 1993 (9,498 cases), with most of the decline resulting from 19.2% fewer cases of rabies in raccoons. two previously described epizootics of rabies involvi ...19957493894
maximal oxygen consumption and pulmonary diffusing capacity: a direct comparison of physiologic and morphometric measurements in canids.the purpose of this study was to check the validity of the morphometric model for estimating physiological conductances for gases, dl. we make a direct comparison between the lung's conductance for carbon monoxide, measured physiologically using the single breath method, dlco (sb), and that measured morphometrically using the previously published model, dlco(mm). we also make a direct comparison between the maximum rate of oxygen uptake by the lung during exercise, vo2max, and the lung's conduct ...19836665333
experimental infections of sarcocystis cruzi, sarcocystis tenella, sarcocystis capracanis and toxoplasma gondii in red foxes (vulpes vulpes).four littermate 6-wk-old red foxes (nos. 1-4) were fed toxoplasma gondii, sarcocystis cruzi, s. tenella and s. capracanis. one littermate fox (no. 5) served as the control. two foxes (nos. 1, 2) were fed tissue cysts of t. gondii and two foxes (nos. 3, 4) were fed oocysts of t. gondii. twenty-one to 42 days later, the same five foxes were used to test the infectivity of meat of goat, sheep, and ox experimentally inoculated with sarcocystis. fox 2 was fed goat meat and shed s. capracanis-like spo ...19836417347
rabies surveillance in the united states during 1993.in 1993, 49 states, the district of columbia, and puerto rico reported 9,495 cases of rabies in nonhuman animals and 3 cases in human beings to the centers for disease control and prevention. greater than 93% (8,889 cases) were wild animals, whereas 6.4% (606 cases) were domestic species. the total number of reported cases increased 9.9% over that of 1992 (8,645 cases), with most of the increase resulting from continued spread of rabies in raccoons (37.1% increase in reported cases over 1992). t ...19947744643
effect of lithium chloride in coyote pup diet. 19807469971
genetic, phenotypic, and behavioral variation in north american sylvatic isolates of trichinella.two restriction endonucleases (cla i and hpa ii) produced polymorphic repetitive dna profiles which were used in a clustering analysis to quantify the level of genetic variation among 14 north american sylvatic isolates of t5 trichinella. differences in genetic profiles reflected phenotypic differences in parasite reproductive success as measured by an isolate's reproductive capacity index in natural hosts. two genetically distinct isolates of the t5 genotype and t. spiralis were used to infect ...19947931904
association of malassezia (pityrosporum) pachydermatis with sarcoptic mange in new york state.malassezia (pityrosporum) pachydermatis consistently accompanied sarcoptic mange in all red foxes (vulpes fulva), porcupines (erethizon dorsatum), and coyotes (canis latrans) examined. this yeastlike microorganism has not heretofore been reported on any of these hosts. its presence on the exoskeleton of sarcoptes scabiei taken from these animals suggests a carrier role for the mite. the yeast may be saprophytic or a secondary pathogen.19806780699
abortion and death in goats inoculated with sarcocystis sporocysts from coyote feces.ten 75- to 105-day-pregnant does each were inoculated orally within 1 million (2 does), 10,000 (4 does), or 1,000 (4 does) sporocysts of sarcocystis from coyote feces. two does not inoculated with sporocysts served as controls. the 2 does inoculated with 1 million sporocysts died from acute sarcocystosis 21 and 22 days after inoculation (dai), and each had 2 dead fetuses. the 4 does inoculated with 10,000 sporocysts were ill 19 to 33 dai but survived; 1 aborted at 33 dai, 1 had a live kid that d ...19816782066
sarcocystis species in moose (alces alces), bison (bison, bison), and pronghorn (antilocapra americana) in montana.the transmission of sarcocystis spp from naturally infected moose, bison, and pronghorn was attempted in laboratory-raised coyotes and dogs. infected musculature from a moose was feed to a dog and a coyote, and 12 days later, both animals shed sporocysts. sporocysts were 14 to 17 x 8.5 to 10.5 micron (av 14.5 x 8.8). a coyote was fed muscle from a bison, and 11 days later, it shed sporocysts. the sporocysts were 14 to 16 x 9 to 11 micron (14.0 x 9.0). infected tissues from a pronghorn were fed t ...19806782919
canine oral papillomavirus genomic sequence: a unique 1.5-kb intervening sequence between the e2 and l2 open reading frames.the canine oral papillomavirus (copv) is associated with oropharyngeal papillomatosis in dogs, coyotes, and wolves. we have determined the complete nucleotide sequence of copv, the largest of all known pv genomes (8607 bp). the genomic architecture of the copv genome is similar to that of other pvs except for a unique and large noncoding region of 1.5 kb between the end of the early region (e2) and the beginning of the late region (l2) and a small (345 bp) upstream regulatory region between the ...19948091677
experimental babesiosis in coyotes and coydogs.one splenectomized and 6 intact coyotes (canis latrans), and 2 coydogs were experimentally inoculated with a recent isolate of babesia gibsoni. the disease was mild in intact animals, was fatal in the splenectomized coyote, and was characterized by a regenerative hemolytic anemia with the pcv decreasing to 16% in intact animals and to 6% in the splenectomized coyote. peak parasitemia ranged from 3% to 21% of erythrocytes infected and was inversely correlated to pcv. serum lactate dehydrogenase, ...19853970435
infectivity of sarcocystis spp. from bison, elk, moose, and cattle for cattle via sporocysts from coyotes.bison bison (bison), cervus canadensis (elk), alces alces (moose), and bos taurus (cattle) musculature containing sarcocystis spp. cysts was fed to laboratory raised canis latrans (coyotes), sporocysts collected from the feces of coyotes fed musculature of each of the ruminant species were fed to four groups of three laboratory-raised domestic calves, respectively, to determine if sarcocystis spp. was transmissible from wild to domestic ruminants and if so, to compare clinical signs of infection ...19826811717
development and ultrastructure of first-generation meronts of sarcocystis cruzi in calves fed sporocysts from coyote feces.the development of sarcocystis cruzi hasselmann (syn. s. fusiformis railliet) meronts was studied in seven 7- to 10-day-old calves filled 4, 7, 11, 15, 22, 25 and 28 days postinoculation (dpi) with 5 x 10(7) sporocysts from feces of coyotes. no meronts were found 4 and 7 dpi. young and intermediate meronts with 1-16 nuclei were found in endothelial cells of arteries in mesenteric lymph nodes, but not in kidneys 11 dpi. mature meronts were noted in endothelial cells of arteries, arterioles, or ca ...19806783745
ticks (acari: ixodidae) collected from small and medium-sized kansas mammals.seven species of hard-bodied ticks were collected from 20 species of small and medium-sized mammals in kansas; amblyomma americanum l., dermacentor variabilis (say), haemaphysalis leporispalustris (packard), ixodes cookei packard, i. kingi bishopp, i. sculptus neumann, and i. texanus banks. dermacentor variabilis was found statewide, a. americanum only in the eastern one-third of the state, and the ixodes spp. and h. leporispalustris were widely scattered. the most common tick found was d. varia ...19948057327
sarcocystosis in neonatal bison fed sarcocystis cruzi sporocysts derived from cattle.a 6-day-old female (bison bison) was inoculated with 10 million sporocysts of the b1 isolate of sarcocystis cruzi originally obtained by feeding heart of a naturally infected cow (bos taurus) to a laboratory-raised coyote. the bison became febrile, lethargic, and anorectic at about 25 days after inoculation of the sporocysts, and was euthanatized 3 days later. there were widespread hemorrhages, hepatitis, myocarditis, nephritis, and enteritis; intravascular meronts were found in the adrenal cort ...19826816777
salmon poisoning disease in juvenile coyotes: clinical evaluation and infectivity of metacercariae and rickettsiae.clinical salmon poisoning disease (spd), and survival of neorickettsia helminthoeca and metacercariae of nanophyetus salmincola in fish were evaluated experimentally in 12-wk-old coyotes (canis latrans) to determine the potential of spd for biological control of coyotes. coyotes readily ate fish that contained metacercariae and rickettsiae. they developed diarrhea, anorexia and lethargy within 7 days after feeding. infected coyotes lost 58% of their body weight when compared to uninfected contro ...19873625898
persistence of a recurrent group of intestinal helminth species in a coyote population from southern texas.the persistence and interrelationships of a recurrent group of intestinal helminth species in a coyote population from southern texas were determined; data were collected at 4-yr intervals (1979, 1983, and 1987). the a priori expectation was that a defined recurrent group of helminth species persisted over time and that the respective helminth species were stable in terms of their abundances. the fager index and subsequent recurrent group analysis indicated a small group of taxonomically unrelat ...19938501593
experimental salmon poisoning disease in juvenile coyotes (canis latrans).salmon poisoning disease (spd) was experimentally induced in juvenile coyotes (canis latrans). the disease was lethal in 11 of 12 coyotes within 15 days after inoculation with 1,000 or 4,000 metacercariae of nanophyetus salmincola. clinical manifestations of the disease included lymph node enlargement, anorexia, pyrexia, diarrhea and death. coccoid bodies indistinguishable from rickettsiae were observed in macrophages of spleen, liver, lymph nodes, and duodenum. percentage recovery of adult trem ...19827097880
heartworms (dirofilaria immitis) in coyotes (canis latrans) in new england. 19827131657
prevalence of neospora caninum and toxoplasma gondii antibodies in coyotes (canis latrans) and experimental infections of coyotes with neospora caninum.antibodies to neospora caninum were detected in 5 (10%) of 52 coyotes from texas. antibodies to toxoplasma gondii were detected in 32 (62%) of 52 samples from these same coyotes. four (80%) of the 5 coyotes that were seropositive for n. caninum also had antibodies to t. gondii. nineteen (37%) of the coyotes did not have antibodies to either parasite. three coyote pups were inoculated with the brains from mice infected with 3 strains of n. caninum originally isolated from dogs. none of the pups d ...19968691381
development of ox-coyote cycle of sarcocystis cruzi. 19826816926
rabies surveillance in the united states during 1995.in 1995, 49 states, the district of columbia, and puerto rico reported 7,877 cases of rabies in nonhuman animals and 4 cases in human beings to the centers for disease control and prevention. nearly 92% (7,247 cases) were wild animals, whereas 8% (630 cases) were domestic species. the total number of reported cases decreased 4.2% from that of 1994 (8,230 cases). most of the decline was the result of 17.1% fewer reported cases of rabies in raccoons in areas of the northeast, where rabies is now e ...19968960176
field use of a vaccinia-rabies recombinant vaccine for the control of sylvatic rabies in europe and north america.during recent years, most research on the control of sylvatic rabies has concentrated on developing methods of oral vaccination of wild rabies vectors. to improve both the safety and the stability of the vaccine used, a recombinant vaccinia virus, which expresses the immunising glycoprotein of rabies virus (vrg), has been developed and tested extensively in the laboratory as well as in the field. from 1989 to 1995, approximately 8.5 million vrg vaccine doses were dispersed in western europe to v ...19969025144
sarcocystosis in newborn calves fed sarcocystis cruzi sporocysts from coyotes.the development of sarcocystis cruzi was compared in 3- to 14-day-old calves (n = 38) inoculated with 55,000 to 500,000,000 sporocysts from intestinal scrapings of coyotes, and euthanatized and necropsied on postinoculation days (pid) 4 to 153. from pid 15 to 19, the calves remained clinically normal, except for fever (greater than or equal to 40 c), during the development of 1st-generation meronts, and lesions in calves euthanatized at this time were confined mainly to mesenteric lymph nodes an ...19826819793
induced toxoplasma gondii, toxocara canis, and isospora canis infections in coyotes.six littermate laboratory-raised coyotes (no. 1-6) were used to study the transmission of toxoplasma gondii from cats and mice, and toxocara canis and isospora canis from dogs and mice. coyotes 1 and 2 were inoculated orally with 1,000 infective oocysts of the tc-1 strain of t gondii; coyotes 3 and 4 were fed mice infected with tissue cysts of the tc-1 strain of t gondii. eight days after inoculation with t gondii, the same 4 coyotes were exposed to toxocara canis: coyotes 1 and 2 were inoculate ...19827174440
pancreatic adenocarcinoma in a coyote-dog cross. 19827154225
serological survey for diseases in free-ranging coyotes (canis latrans) in yellowstone national park, wyoming.from october 1989 to june 1993, we captured and sampled 110 coyotes (canis latrans) for various diseases in yellowstone national park, wyoming (usa). prevalence of antibodies against canine parvovirus (cpv) was 100% for adults (> 24 months old), 100% for yearlings (12 to 24 months old), and 100% for old pups (4 to 12 months old); 0% of the young pups (< 3 months old) had antibodies against cpv. presence of antibodies against canine distemper virus (cdv) was associated with the age of the coyote, ...19979027690
prevalence of heartworm (dirofilaria immitis leidy) in coyotes from five northern california counties.prevalence rates of heartworm in coyotes was estimated for five northern california counties by examining hearts of coyotes obtained from federal or private trappers. prevalence rates range from 8.3-27.3%, but the proportion of infected coyotes can vary between two regions of the same county. in general, it seems that higher infection rates are found in the foothill regions of the coastal range and sierra nevada, decreasing towards the floor of the sacramento valley as well as at higher elevatio ...19827125062
rabies surveillance in the united states during 1996.in 1996, 49 states, the district of columbia, and puerto rico reported 7,124 cases of rabies in non-human animals and 4 cases in human beings to the centers for disease control and prevention. nearly 92% (6,550 cases) were wild animals, whereas 8% (574 cases) were domestic species. the total number of reported cases decreased 9.6% from that of 1995 (7,881 cases). although much of the decline was the result of fewer reported cases of rabies in raccoons, fewer cases were also reported among most g ...19979412679
attempted experimental transfer of sarcoptic mange (sarcoptes scabiei, acarina: sarcoptidae) among red fox, coyote, wolf and dog. 19817310942
serologic evidence of leptospirosis in a southern arizona coyote population. 19817253099
environmental exposure to coxiella burnetii: a sero-epidemiologic survey among domestic animals.the prevalence of agglutinating serum antibodies against coxiella burnetii, the cause of q fever in humans, was tested in a hospital population of companion animals and livestock in california during 1973--1975. a sample of stray dogs was also tested. among the hospitalized animals 346 (48%) of 724 dogs, 7 (9%) of 80 cats, 9 (32%) of 28 cattle and 31 (26%) of 121 horses had antibodies against c. burnetii. of 316 stray dogs 208 (66%) were seropositive. the overall prevalence of 53% among 1040 dog ...19807377186
hammondia heydorni infection in sheep, goats, moose, dogs and coyotes.the transmission of hammondia heydorni among sheep, goats, cattle, dogs and coyotes is described. oocysts of h. heydorni, obtained from dog faeces by feeding naturally infected goat meat, were fed to an experimental goat. after 312 days tissues from this animal were fed to 2 dogs. one dog, which received goat muscle, shed oocysts, whereas the other dog which received brain, spleen, kidneys, lungs and liver did not. oocysts from the dog fed goat muscle were fed to an experimental sheep. after 73 ...19807422358
translocation of coyote rabies--florida, 1994.translocation of a rabies variant from one area to another has been identified increasingly in the united states. during november and december 1994, rabies was diagnosed in five dogs from two associated kennels in florida; in addition, two other dogs being kept at one of the kennels died with suspected, but unconfirmed, rabies. rabies virus recovered from the five dogs was identified as a variant not previously found in florida but endemic in coyotes (canis latrans) in south texas. the suspected ...19957623760
coyotes and canine heartworm in california.adult dirofilaria immitis were found in the hearts of 43 of 115 coyotes (canis latrans) trapped within a 50 km radius of the el dorado county seat. of mature coyotes, 45% were positive, with worm numbers averaging 9.0 in females and 16.2 in males. d. immitis microfilariae occurred in the peripheral blood and in lung smears. microfilariae of dipetalonema reconditum were present in 14 of the coyotes examined (12%). several coyotes showed enlarged hearts with gross pathological changes.19807431518
epizootic canine rabies transmitted by coyotes in south texas.prior to 1988, rabies was reported only sporadically in coyotes. however, in the final 4 months of 1988, starr county, tex, which is situated on the us-mexico border, experienced an epizootic of canine rabies, consisting of 6 laboratory-confirmed cases of rabies in coyotes and of 2 cases in domestic dogs. the first 3 cases were detected in coyotes, and the first case in a domestic dog was observed 84 days after the index case. adjacent hidalgo county reported 9 cases of rabies in dogs during the ...19948163414
rabies surveillance in the united states during 1997.in 1997, 49 states, the district of columbia, and puerto rico reported 8,509 cases of rabies in nonhuman animals and 4 cases in human beings to the centers for disease control and prevention. nearly 93% (7,899) were wild animals, whereas 7% (610) were domestic species. the total number of reported cases increased 19.4% from that of 1996 (7,128 cases). increases were apparent in each of the major species groups, with the exception of cattle. the relative contributions of these groups to the total ...19989861958
phenotypic characterization of human and animal biotypes within the species porphyromonas gingivalis.ninety-nine strains of gram-negative black-pigmented anaerobic rods, grown on todd-hewitt blood agar plates, were identified and characterized according to a typing scheme including uv fluorescence, catalase, trypsin-like and haemagglutinating activities, biochemical tests with the atb 32a kit, and gas-liquid chromatography. to determine the taxonomic position of the porphyromonas gingivalis biotypes, 68 strains (31 of human origin and 37 of animal origin) were compared to 31 strains of closely ...19938190990
naturally occurring hepatozoonosis in coyotes from oklahoma.nine of 16 free-ranging coyotes (canis latrans) from central oklahoma (usa) had naturally acquired infections of hepatozoon americanum. infections were confirmed by recognition of tissue stages closely resembling h. americanum in skeletal and cardiac muscle. at the time coyotes were collected they were infested with a variety of ticks, including adult gulf coast ticks (amblyomma maculatum). we propose that the high prevalence of h. americanum in this small sample of free-ranging coyotes and the ...199910073352
structure and expression of a divergent canine class i gene.we have isolated and characterized a canine class i mhc (dog leukocyte ag, dla) gene, dla-79. the deduced protein sequence shares only 65% identity with a previously published canine class i cdna, designated dla-a, and exhibits 64% amino acid identity with the hla-a, -b, -c consensus. the peptide-binding region of dla-79 is unusual. three of four highly conserved tyrosine residues (tyr7, 59, 159, and 171), proposed to interact with the n terminus of peptide-ag, are substituted. additionally, the ...19957594586
field application of the conditioned taste aversion paradigm to the control of coyote predation on sheep and turkeys. 19807447856
quantitative parasitemia in calves fed sarcocystis cruzi sporocysts from coyotes.for the quantitation of parasitemia, seven 7- to 10-day-old calves were orally inoculated with 2 virulent isolates of sarcocystis cruzi from coyotes. three calves were each given 2.5 million sporocysts of the 1st isolate; these calves died 31, 35, and 33 days later. the other 4 calves were given 2, 1, 0.76, and 0.38 million sporocysts of the 2nd isolate; these calves died or were euthanatized at 34, 44, 43, and 55 days after inoculation. buffy coat smears from 7 ml of jugular blood of each calf ...19826808868
sarcocystis of deer in south dakota.the prevalence of sarcocystis in white-tailed deer (odocoileus virginianus) and mule deer (o. hemionus) in south dakota was determined through microscopic examination of tongue samples. the percentage of sarcocystis infection for both species of deer was determined for prairies east of the missouri river, west of the missouri river, and black hills of western south dakota. sixteen percent (n = 62) of the white-tailed deer tongues from east river, 69% (n = 42) from west river, and 74% (n = 23) fr ...19826808161
sarcoptes scabiei infestation of the coyote (canis latrans), illustrated by the scanning electron microscopy. 19826808163
molecular genetics of the most endangered canid: the ethiopian wolf canis simensis.the world's most endangered canid is the ethiopian wolf canis simensis, which is found in six isolated areas of the ethiopian highlands with a total population of no more than 500 individuals. ethiopian wolf populations are declining due to habitat loss and extermination by humans. moreover, in at least one population, ethiopian wolves are sympatric with domestic dogs, which may hybridize with them, compete for food, and act as disease vectors. using molecular techniques, we address four questio ...19947921357
molecular evolution of the dog family.molecular genetic tools have been used to dissect the evolutionary relationships of the dog-like carnivores, revealing their place in the order carnivora, the relationships of species within the family canidae, and the genetic exchange that occurs among conspecific populations. high rates of gene flow among populations within some species, such as the coyote and gray wolf, have suppressed genetic divergence, and where these species hybridize, large hybrid zones have been formed. in fact, the phe ...19938337763
biochemical, biological, and genetic characterization of a sylvatic isolate of trichinella.biological, biochemical, and genetic relationships of trichinella isolates were assessed and compared from 3 species of illinois fur-bearing mammals. tongue muscle collected from 1987 through 1989 from 323 raccoons (procyon lotor), 9 red fox (vulpes fulva), and 1 coyote (canis latrans) were digested and trichinella muscle larval prevalences and mean intensities (larvae/g) determined. the prevalence and mean intensity of tongue muscle-stage larvae were 2.8% and 44.4% and 326 and 2 larvae/g for ra ...19938501590
naturally occurring and experimentally transmitted hepatozoon americanum in coyotes from oklahoma.twenty free-ranging coyotes (canis latrans) in oklahoma (usa) were examined for the presence of naturally occurring infections with hepatozoon americanum and to determine if bone lesions attributable to h. americanum were present. although eight of the 20 free-ranging coyotes were found to be naturally infected with h. americanum, no bone lesions were detected. in addition, two coyote pups were exposed to h. americanum oocysts collected from experimentally infected ticks and the course of the re ...200010682757
evaluation of fox-chasing enclosures as sites of potential introduction and establishment of echinococcus multilocularis.following detection of echinococcus multilocularis in red foxes (vulpes vulpes) illegally imported into south carolina (usa) for release in fox-chasing enclosures, a survey for e. multilocularis was conducted in four enclosures in georgia (usa) and six enclosures in south carolina. survey methods included examination of potential small mammal intermediate hosts (n = 390) for e. multilocularis larvae, examination of fox and coyote (canis latrans) scats (n = 59) for taeniid eggs, and examination o ...19938355358
characterization of a unique variant of bat rabies virus responsible for newly emerging human cases in north america.the silver-haired bat variant of rabies virus (shbrv) has been identified as the etiological agent of a number of recent human rabies cases in the united states that are unusual in not having been associated with any known history of conventional exposure. comparison of the different biological and biochemical properties of isolates of this virus with those of a coyote street rabies virus (cosrv) revealed that there are unique features associated with shbrv. in vitro studies showed that, while t ...19968643632
plague in free-ranging mammals in western north dakota.from july through october of 1996, 48 blood samples were collected from coyotes (canis latrans), badgers (taxidea taxus), and raccoons (procyon lotor) in western north dakota (usa) for the purposes of determining antibody titers to the plague bacterium, yersniia pestis. the passive hemagglutination paper-strip blood-sampling technique was utilized with hemagglutination inhibition controls. two positive samples were obtained from mckenzie county, one from a coyote with a titer of 1:64 and one fro ...199910479100
a noninvasive method for distinguishing among canid species: amplification and enzyme restriction of dna from dung.endangered san joaquin kit foxes vulpes macrotis mutica can be sympatrically distributed with as many as four other canids: red fox, gray fox, coyote and domestic dog. canid scats are often found during routine fieldwork, but cannot be reliably identified to species. to detect and study the endangered kit fox, we developed mitochondrial dna markers that can be amplified from small amounts of dna extracted from scats. we amplified a 412-bp fragment of the mitochondrial cytochrome-b gene from scat ...19979161016
restriction fragment length polymorphism analysis of mycobacterium bovis isolates from captive and free-ranging animals.mycobacterium bovis isolates from cattle, captive elk, and free-ranging mule deer and coyotes were examined by restriction fragment length polymorphism (rflp) analysis. dna extracted from each isolate was digested with restriction endonucleases alui and pvuii. dna probes used for southern hybridizations were a 37-base oligonucleotide and a 123-base-pair sequence specific for the insertion sequence is6110 and a plasmid, ptbn12, which contains a polymorphic gc-rich repetitive sequence present in s ...19979376427
echinococcus multilocularis identified in indiana, ohio, and east-central illinois.echinococcus multilocularis was identified in a coyote in indiana in january 1990. subsequently, 300 wild canids (red foxes, gray foxes, coyotes) were collected from indiana, ohio, illinois, michigan, and kentucky and examined for this parasite. of these, 41 (13.7%) were infected with e. multilocularis. infected animals were from northern and central indiana, northwestern ohio, and east-central illinois. twenty-nine of 162 (17.9%) animals from northern and central indiana were positive, versus 0 ...19938459347
molecular epidemiology of rabies epizootics in texas.texas is in the midst of two independent epizootics of rabies, involving coyotes (canis latrans) and domestic dogs (canis familiaris) in southern texas and grey foxes (urocyon cinereoargenteus) in west central texas. the domestic dog/coyote (ddc) and grey for (tf) rabies virus variants cannot be differentiated by antigenic typing with currently available monoclonal antibodies. these two variants also cannot be distinguished from a third variant, sonora dog (sd) rabies, that is not enzootic in te ...19979406651
responses of captive and free-ranging coyotes to simulated oral rabies vaccine baits.the objective of this study was to develop a bait for delivering an oral rabies vaccine to free-ranging coyotes (canis latrans) in southern texas. captive trials were conducted from january to april, 1994, to determine bait preferences and behavioral responses of coyotes (n = 42) to selected baits and attractants. baits were hollow rectangular cubes made of polymer dog food or fish meal. attractants had sweet (watermelon), fruity (raspberry), sulfurous (synthetic wu), and lard (beef lard) fragra ...19989476221
spatial associations among density of cattle, abundance of wild canids, and seroprevalence to neospora caninum in a population of beef calves.to determine the epidemiologic plausibility of a sylvatic transmission cycle for neospora caninum between wild canids and beef cattle.200011061391
efficacy of bait distributional strategies to deliver canine rabies vaccines to coyotes in southern texas.this study sought to develop a baiting strategy to deliver an oral rabies vaccine to free-ranging coyotes (canis latrans) in southern texas. to determine bait longevity, dog foodlard baits were placed (n = 50) on- and off-roads during july 1994 and january 1995. coyote visitation and uptake rates did not differ between on-road and off-road placement of baits. to evaluate bait stations as possible visual cues, baits were placed out both with (n = 50) and without (n = 50) bait stations. a visual c ...19989476222
winter poisoning of coyotes and raptors with furadan-laced carcass baits.three bald eagles (haliaeetus leucocephalus), a red-tailed hawk (buteo jamaicensis), and two coyotes (canis latrans) found in a field in north-central kansas (usa) in december 1992 were poisoned by flowable carbofuran (furadan 4f) placed on sheep (ovis aries) carcasses to kill coyotes. the carbofuran was placed on the carcasses in october 1992, but the coyotes and raptors apparently were killed in late december. thus, flowable furadan can cause direct and secondary deaths of wildlife under some ...19968722286
trichinella spiralis in sylvatic hosts from prince edward island.larvae of trichinella sp. were found in two of 208 red foxes (vulpes vulpes) and one of 125 coyotes (canis latrans) obtained from trappers from prince edward island (canada) in 1995 and 1996. a polymerase chain reaction based dna biotyping method revealed the larvae to be isolates of trichinella spiralis. this is the first verified identification of t. spiralis in sylvatic hosts from canada.19989476239
american canine hepatozoonosis. an emerging disease in the new world.hepatozoon canis was first described from dogs in 1905 in india and rhipicephalus sanguineus was identified as the vector. dogs on the texas gulf coast were recognized in 1978 to have hepatozoonosis, and it was thought that h. canis had entered the new world. later, it was realized that american canine hepatozoonosis (ach) is more debilitating than its old world counterpart, often resulting in death. when the malady and parasite were characterized, a new species, h. americanum, was described, in ...200011193705
neosporosis. aspects of epidemiology and host immune response.neospora caninum is a recently recognized protozoan parasite which has been described as causing a neuromuscular paralysis in dogs and is emerging as a major cause of bovine infertility and abortion worldwide. the parasite is known to infect a range of warm blooded animals but the disease predominates in dogs and cattle. it is not yet known if n. caninum can infect and cause disease in people. the dog has recently been identified as the definitive host and the parasite may be transmitted through ...200011193706
pseudorabies in captive coyotes.pseudorabies (aujeszky's disease) was diagnosed in three adult captive coyotes (canis latrans) from southern indiana (usa). the coyotes died in their outdoor enclosure within a 48 hr period. histopathology revealed multifocal, nonsuppurative meningioencephalitis and eosinophilic intranuclear inclusion bodies within neurons. samples of brain were positive for pseudorabies virus by fluorescent antibody testing and virus isolation. source of infection was the probable consumption of pseudorabies vi ...19979391985
identification of opossums (didelphis virginiana) as the putative definitive host of sarcocystis neurona.sarcocystis neurona is an apicomplexan that causes equine protozoal myeloencephalitis (epm) in north and south america. horses appear to be an aberrant host, because the merozoites continually divide in the central nervous system, without encysting. the natural host species has not previously been identified. the small subunit ribosomal rna (ssurna) gene of s. neurona was compared to those of sarcocystis muris, sarcocystis cruzi, toxoplasma gondii, and cryptosporidium parvum to identify a unique ...19958544064
serologic survey for hantavirus infection in domestic animals and coyotes from new mexico and northeastern arizona.to determine whether animals had serologic evidence of infection with sin nombre virus (snv).19989540866
host association and seasonal activity of amblyomma americanum (acari: ixodidae) in missouri.from june 1993 through june 1996, 2,260 adult, 4,426 nymphal, and 2,178 larval lone star ticks amblyomma americanum (l.) were collected in missouri from vertebrate hosts and by dragging a cloth over vegetation. prevalence, mean intensity, and relative abundance of each stage varied among hosts. the relative abundance of adult lone star ticks was highest on white-tailed deer, but this stage was also collected from raccoons, opossum, red fox, coyotes, and wild turkey. nymphs were collected from gr ...200011128501
viral antibodies in coyotes from california.prevalence of antibodies against canine parvovirus (cpv), canine distemper virus (cdv), and canine adenovirus type 1 (cav) were determined among 152 coyotes (canis latrans) at the naval petroleum reserves (nprc; california, usa) from 1985 to 1990. overall prevalence of antibodies to cpv, cdv, and cav was 66%, 37%, and 68%, respectively. prevalence of cpv and cdv varied significantly among years. antibody prevalence did not differ between sexes for any disease, but did vary significantly among ag ...19989577772
trichinella infection in wildlife of the southwestern united states.several potential mammalian reservoirs of sylvatic species of trichinella were examined from texas, new mexico, and arizona. during 1998-99, tongues were collected from a black bear (ursus americanus) in arizona; from 9 black bears, a coyote (canis latrans), and a mountain lion (felis concolor) in new mexico; and from 154 coyotes, 32 raccoons (procyon lotor), 13 opossums (didelphis marsupialis), 4 ocelots (leopardus pardalis), 3 bobcats (lynx rufus), and 5 feral hogs (sus scrofa) in southern tex ...200111695403
typing of rabies virus isolates by dna enzyme immunoassay.alternatives to antigenic typing are needed for epidemiologic surveys of the rabies virus associated with translocated coyotes and foxes, especially in areas where a closely related rabies virus is transmitted by striped skunks.199910073409
increasing prevalence of canine heartworm in coyotes from california.thirty-seven subadult and adult coyotes (canis latrans), collected august 1992 through december 1996 from a coastal foothill area in northern california (usa), were examined for adult heartworm (dirofilaria immitis). during 1992 through 1993, at the end of a 6 yr drought, none of four coyotes examined were infected with heartworms. however, during 1994 through 1996, after the drought had ended, prevalences were 91% in 23 adult coyotes and 40% in 10 subadult coyotes. heartworm intensity did not d ...19989577791
effect of short-term coyote removal on populations of coyote helminths.coyote (canis latrans) removal programs often are initiated despite the potential population regulatory mechanism of parasitism with increased coyote density. we investigated the effect of intensive, short-term coyote removal on population levels of helminths in juvenile and adult coyotes from western texas. coyotes were killed by aerial gunning every 3 mo for 2 yr on two 5,000 ha areas, which reduced the overall coyote density of these areas by about 50%. two other 5,000 ha areas were used as c ...200211838229
toxoplasma gondii antibodies in naturally exposed wild coyotes, red foxes, and gray foxes and serologic diagnosis of toxoplasmosis in red foxes fed t. gondii oocysts and tissue cysts.antibodies to toxoplasma gondii were determined in sera from 222 coyotes (canis latrans), 283 red foxes (vulpes vulpes), and 97 gray foxes (urocyon cinereoargenteus) from indiana, kentucky, michigan, and ohio during 1990-1993. sera were examined in 1:25, 1:100, and 1:500 dilutions by the modified direct agglutination test (mat) with formalinized whole tachyzoites plus mercaptoethanol. antibodies were found in 131 (59.0%) of 222 coyotes, 243 (85.9%) of 283 red foxes, and 73 (75.3%) of 97 gray fox ...199910219302
mycobacterium bovis in coyotes from michigan.during a survey for tuberculosis in wild carnivores and omnivores, mycobacterium bovis was cultured from pooled lymph nodes of three adult female coyotes (canis latrans) harvested by hunters in michigan (usa). no gross or histologic lesions suggestive of tuberculosis were seen in these animals. one coyote was taken from montmorency county and two coyotes from alcona county located in the north-eastern portion of michigan's lower peninsula where free-ranging white-tailed deer (odocoileus virginia ...19989706576
rabies surveillance in the united states during 2000.during 2000, 49 states, the district of columbia, and puerto rico reported 7,364 cases of rabies in nonhuman animals and 5 cases in human beings to the centers for disease control and prevention, an increase of 4.3% from 7,067 cases in nonhuman animals reported in 1999. ninety-three percent (6,855 cases) were in wild animals, whereas 6.9% (509 cases) were in domestic species (compared wth 91.5% in wild animals and 8.5% in domestic species in 1999). compared with cases reported in 1999, the numbe ...200111767918
health evaluation of a pronghorn antelope population in oregon.during 1996 and 1997, the u.s. fish and wildlife service conducted a study to determine the cause(s) of population decline and low survival of pronghorn antelope (antilocapra americana) fawns on hart mountain national antelope refuge (hmnar) located in southeastern oregon (usa). as part of that study, blood, fecal, and tissue samples from 104 neonatal fawns, 40 adult does, and nine adult male pronghorns were collected to conduct a health evaluation of the population. physiological parameters rel ...199910479084
use of anti-glycoprotein monoclonal antibodies to characterize rabies virus in formalin-fixed tissues.seventy anti-rabies virus monoclonal antibodies (mabs) were tested for reactivity with rabies and rabies-related viruses in formalin-fixed (ff) tissues. forty-three of the mabs were directed against the glycoprotein and 27 were directed against the nucleocapsid as determined by enzyme immunoassays and neutralization tests. twenty of the anti-glycoprotein mabs and one of the anti-nucleocapsid mabs reacted with the rabies challenge virus strain (cvs) in ff tissue. these 21 mabs were screened again ...199910029326
allozyme variability in the italian wolf (canis lupus) population.multilocus protein electrophoresis was used to estimate genetic variability in a sample of 38 italian wolves (canis lupus). percentage of polymorphic loci was p = 10.0 per cent (four polymorphic loci out of 40 examined), and average observed heterozygosity was ho = 0.028. genotypes were in hardy-weinberg equilibrium. electrophoretic analysis does not indicate a significant reduction of genetic variability at nuclear gene loci following at least one century of isolation from other european popula ...19938276634
seasonal activity and host associations of ixodes scapularis (acari: ixodidae) in southeastern missouri.based on tick collections recovered from wild vertebrates and by dragging, the seasonal occurrence of adult blacklegged ticks, ixodes scapularis say, extended from october through may in southeastern missouri. adult activity was bimodal with the higher peak occurring in november followed by a lower peak in february. the activity of immature i. scapularis had the general pattern of that found in the northeast where lyme disease is hyperendemic, with larval activity (july) peaking after that of ny ...199910593072
failure to identify alveolar echinococcosis in trappers from south dakota in spite of high prevalence of echinococcus multilocularis in wild canids.echinococcus multilocularis causes a rare but potentially lethal zoonotic disease in humans. this tapeworm has been known to be endemic in foxes (vulpes vulpes) and coyotes (canis latrans) within the northern united states since the 1960s. one purpose of this study was to provide recent data on the prevalence of e. multilocularis in foxes and coyotes from eastern south dakota. in a survey conducted from 1987 to 1991 and involving 137 foxes and 9 coyotes from this area, 74.5% of the foxes and 4 o ...200010701567
rabies surveillance in the united states during 1992.in 1992, 49 states, the district of columbia, and puerto rico reported 8,644 cases of rabies in nonhuman animals and 1 case in a human being to the centers for disease control and prevention. almost 92% (7,912 cases) were wild animals, the largest number of wild animals ever reported, whereas 8.5% (732 cases) were domestic species. the total number of reported cases increased 23.9% over that of 1991 (6,975 cases), with most of the increase resulting from continued spread of rabies in raccoons. t ...19938307825
polymorphism of alpha 1-antitrypsin in north american species of canis.alpha 1-antitrypsin (a1at) is a major protease inhibitor present in all mammalian sera that have thus far been investigated. a1at is also highly polymorphic and is therefore a useful genetic marker. previously reported a1at polymorphism in domestic dogs consisted of two alleles designated as pim and pis which exhibited frequencies of 0.72 and 0.28, respectively, in a group of randomly collected mongrel dogs. north american species of canis, which included gray wolves (n = 29), mexican wolves (n ...200010768138
molecular systematics of mesocestoides spp (cestoda: mesocestoididae) from domestic dogs (canis familiaris) and coyotes (canis latrans).the genus mesocestoides vaillant, 1863 includes tapeworms of uncertain phylogenetic affinities and with poorly defined life histories. we previously documented 11 cases of peritoneal cestodiasis in dogs (canis familiaris l.) in western north america caused by metacestodes of mesocestoides spp. in the current study, dna sequences were obtained from metacestodes collected from these dogs (n = 10), as well as proglottids from dogs (n = 3) and coyotes (canis latrans say, 1823 [n = 2]), and tetrathyr ...200010780557
skeletal lesions of canine hepatozoonosis caused by hepatozoon americanum.canine hepatozoonosis, caused by hepatozoon americanum, is an emerging tick-borne disease of dogs in north america. in addition to the skeletal and cardiac myositis that are prominent features of the disease, there is disseminated periosteal bone proliferation in most dogs that manifest clinical disease. each of six experimentally infected animals (four dogs and two coyotes) and seven of eight naturally infected dogs had gross or histopathologic osteoproliferative lesions. experimental animals w ...200010810986
serologic and molecular evidence of ehrlichia spp. in coyotes in california.in order to determine the role of coyotes in the epidemiology of granulocytic and monocytic ehrlichial agents in california (usa), we tested 149 serum samples for antibodies against ehrlichia equi, e. risticii, and e. canis, using an indirect immunofluorescent antibody test. polymerase chain reaction (pcr) assay was used to survey for the presence of members of the e. phagocytophila genogroup, e. risticii and e. canis in blood samples of 95 coyotes. sixty-eight (46%) samples were seropositive fo ...200010941735
species differences in normal brain cholinesterase activities of animals and birds.the normal cholinesterase activity in brain tissue was measured in 15 mammalian and 44 avian species using the ellman method. enzyme activity exhibited considerable interspecies variability. in mammals, the enzyme activities ranged from approximately 2 to 10 micromole/min/g of wet tissue. with the exception of the carnivores (dog, fox, coyote), no consistency of the enzyme activity could be identified in related mammalian species. the range of interspecies differences associated with avian choli ...200212046961
rabies surveillance in the united states during 2001.during 2001, 49 states and puerto rico reported 7,437 cases of rabies in nonhuman animals and 1 case in a human being to the centers for disease control and prevention, an increase of < 1% from 7,364 cases in nonhuman animals and 5 human cases reported in 2000. more than 93% (6,939 cases) were in wild animals, whereas 6.7% (497 cases) were in domestic species (compared with 93.0% in wild animals and 6.9% in domestic species in 2000). the number of cases reported in 2001 increased among bats, cat ...200212494966
spatial analysis of yersinia pestis and bartonella vinsonii subsp. berkhoffii seroprevalence in california coyotes (canis latrans).zoonotic transmission of sylvatic plague caused by yersinia pestis occurs in california, usa. human infections with various bartonella species have been reported recently. coyotes (canis latrans) are ubiquitous throughout california and can become infected with both bacterial agents, making the species useful for surveillance purposes. this study examined the geographic distribution of 863 coyotes tested for y. pestis and bartonella vinsonii subsp. berkhoffii serologic status to gain insight int ...200312507856
seroepidemiology of bartonella vinsonii subsp. berkhoffii infection in california coyotes, 1994-1998.the prevalence of antibodies to bartonella vinsonii subsp. berkhoffii in coyotes (canis latrans) in california ranged from 51% in central to 34% in southern and 7% in northern california. seropositive coyotes were more likely to be from coastal than inland counties (p clustered distribution of bartonella seropositivity in coyotes suggests that b. vinsonii subsp. berkhoffii infection is vectorborne. further investigation is warranted to evaluate which arthropods are vectors and what the mode of t ...199910511529
naturally occurring ehrlichia chaffeensis infection in coyotes from oklahoma.a nested polymerase chain reaction assay was used to determine the presence of ehrlichia chaffeensis, e. canis, and e. ewingii dna in blood samples of free-ranging coyotes from central and northcentral oklahoma. of the 21 coyotes examined, 15 (71%) were positive for e. chaffeensis dna; none was positive for e. canis or e. ewingii. results suggest that e. chaffeensis infections are common in free-ranging coyotes in oklahoma and that these wild canids could play a role in the epidemiology of human ...200010998377
canines as sentinels for lyme disease in san diego county, california.prevalence of lyme borreliosis in canine sentinels has been shown to correlate with infection in humans. one thousand canine sera (917 dogs, 83 coyotes) obtained from animal control authorities and area veterinarians were screened by elisa for antibodies to borrelia burgdorferi. results were validated by western blot and indirect fluorescent antibody (ifa) tests at referee laboratories. criterion for a positive western blot was presence of 5 of 10 of the most common antigen igg bands; for ifa, > ...200010730940
coyotes (canis latrans) as the reservoir for a human pathogenic bartonella sp.: molecular epidemiology of bartonella vinsonii subsp. berkhoffii infection in coyotes from central coastal california.bartonella vinsonii subsp. berkhoffii was originally isolated from a dog suffering infectious endocarditis and was recently identified as a zoonotic agent causing human endocarditis. following the coyote bite of a child who developed clinical signs compatible with bartonella infection in santa clara county, calif., this epidemiological study was conducted. among 109 coyotes (canis latrans) from central coastal california, 31 animals (28%) were found to be bacteremic with b. vinsonii subsp. berkh ...200011060089
muscular sarcocystosis in coyotes from oklahoma.in a recent survey in oklahoma (usa), 52 free-ranging coyotes were examined for the presence of sarcocysts. two of these coyotes were found infected with sarcocysts in skeletal muscle. by light microscopy, the cyst wall was thin and smooth. ultrastructurally, the cyst wall had minute villar protrusions. the sarcocysts were 14.4 to 50.4 microm wide and 46.8 to 99 microm long. this is the first report of sarcocystis sp. sarcocysts in the skeletal muscle of coyotes.200011085440
bovine tuberculosis in free-ranging carnivores from michigan.during a survey of carnivores and omnivores for bovine tuberculosis conducted in michigan (usa) since 1996, mycobacterium bovis was cultured from lymph nodes pooled from six coyotes (canis latrans) (four adult female, two adult male), two adult male raccoons (procyon lotor), one adult male red fox (vulpes vulpes), and one 1.5-yr-old male black bear (ursus americanus). one adult, male bobcat (felis rufus) with histologic lesions suggestive of tuberculosis was negative on culture but positive for ...200111272505
q fever in humans and animals in the united states.coxiella burnetii, the etiologic agent of q fever, is a worldwide zoonotic pathogen. although q fever is present in the united states, little is known about its current incidence or geographic distribution in either humans or animals. published reports of national disease surveillance, individual cases, outbreak investigations, and serologic surveys were reviewed to better characterize q fever epidemiology in the united states. in national disease surveillance reports for 1948-1986, 1,396 human ...200212737547
an apparatus for studying operant activity of captive coyotes.we describe a portable apparatus designed to examine the free-operant food preferences of captive coyotes in their home kennels. because lever-pressing for food access was the dependent variable, we measured food preference independently of food ingestion. using successive approximation, we trained 8 out of 19 coyotes (42%) to use the apparatus. this percentage is similar to training rates for dogs. we used fixed and variable ratio schedules of reinforcement to further test 4 of the trained coyo ...200011189857
major histocompatibility complex (mhc) variation in the endangered mexican wolf and related canids.we have examined in mexican wolves and related canids the amount of genetic variation for a class ii gene in the major histocompatibility complex (mhc), thought to be part of the most important genetic basis for pathogen resistance in vertebrates. in mexican wolves, descended from only seven founders over three lineages, there were five different alleles. these were in three phylogenetic groups, only one of which was shared between lineages. using single stand conformation polymorphism (sscp), w ...200011240629
morbidity-mortality factors and survival of an urban coyote population in arizona.the health of coyotes (canis latrans) in urban areas has not been studied. our objectives were to assess the health of coyotes in tucson (arizona, usa) by determining the prevalence of antibodies to selected pathogens, estimating survival rates, and identifying sources of mortality. we drew blood from 22 coyotes to evaluate the prevalence of heartworm (dirofilaria immitis) antigens, and antibodies to canine distemper virus (cdv), infectious canine hepatitis (ich), canine parvovirus (cpv), and se ...200111310882
serological responses of coyotes to two commercial rabies vaccines.between august 1993 and september 1994 we documented serological responses of coyotes (canis latrans) vaccinated with two commercial rabies vaccines licensed for use in domestic dogs. serologic responses were documented by testing for rabies virus neutralizing antibodies with the rapid fluorescent focus inhibition test (rffit) at 30, 90, 180, 270, and 365 days post-vaccination. all coyotes vaccinated with imrab 3 (rhone-merieux, inc.), and 75% of those vaccinated with dura-rab 3 (immunovet, inc. ...200111763743
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