| properties of permissive monkey cells transformed by uv-irradiated simian virus 40. | african green monkey cells (cv1 line) were infected with uv-irradiated simian virus 40 (sv40), and permissive lines of stably transformed cells were established. these cell lines display the sv40 t-antigen and the growth characteristics typical of nonpermissive transformed cells (e.g., reduced cell density inhibition, reduced serum dependence, ability to overgrow normal cells, and colony formation in soft agar). the level of permissiveness to superinfecting sv40 is fully comparable with that of ... | 1977 | 194053 |
| linkage relationship between the genes for thymidine kinase and galactokinase in different primates. | in this study we investigated the expression of primate galactokinase in somatic cell hybrids between a thymidine kinase-deficient mouse cell line and two different primate cell lines, one of which was derived from african green monkey kidney cells and the other from chimpanzee fibroblasts. all the african green monkey-mouse hybrid clones, selected in hat medium, expressed monkey galactokinase activity and contained a monkey chromosome similar to a human e-group chromosome. when these clones wer ... | 1976 | 195352 |
| african green monkey fibroblast actin morphology during sv40 infection. | monkey skin fibroblasts were infected with simian virus 40. cells that exhibited the viral tumor antigen were found to retain the normal morphology of actin filaments up to six days after infection. however when cells were transformed in terms of focus formation they had lost the normal actin morphology. | 1977 | 195416 |
| mycoplasma in african green monkey kidney cell cultures: biochemical detection and effects in virus-infected cells. | among a number of techniques for the detection of mycoplasmal contamination in african green monkey kidney (agmk) cell lines, the assay of uridine phosphorylase activity is unsuitable because of the presence of high levels of endogenous enzymatic activity. a thymidine phosphorylase test, however, based on the chromatographic analysis of radiolabeled thymidine breakdown, turned out to be a simple and sensitive mycoplasma detection method. we found, using the latter technique, that 0.22-micrometer ... | 1977 | 195895 |
| characterization of simian virus 40 transformed african green monkey cells (cv-1). i. defective virion and viral genome. | several clones of sv40 transformed cv-1 cells have been characterized for the production of t- and v-antigens and for the state of viral genome. the transformed cv-1 cells failed to produce infectious virions as assayed after sonication or cocultivation and fusion with normal cv-1 cells, and were resistant to super-infection by sv40. some clones of the transformed cells contained v-antigens. the population of v-antigen positive cells varied from 0 to 100% depending on the passage number while th ... | 1977 | 196162 |
| [demonstration by immunofluorescence of adenohyphysis corticotropin and melanotropin cells in erythrocebus patas, cercopithecus aethiops and papio hamadryas]. | using antibodies against synthetic corticotropic hormones (1-24 acth and 17-39 acth), and melanotropic hormones (alpha-msh and beta-msh), it is possible to identify corticotropic and melanotropic cells in the adenohypophysis of three species of monkeys : erythrocebus patas, cercopithecus aethiops and papio hamadryas. the corticotropic cells are numerous in the anterior lobe in both the adult and infant male and female monkeys of these three species. the intermediate lobe reacts with antibodies a ... | 1977 | 196718 |
| structure and formation of circular dimers of simian virus 40 dna. | most of the viral dna extracted from simian virus 40 (sv40)-infected african green monkey kidney cells consists of circular molecules about 5.3 kilobases in contour length. however, about 1% of the viral dna was found to occur as closed circular dimers that appeared to be formed, preferentially, late in infection. the monomeric units of dimers were organized in a head-to-tail, tandem arrangement; moreover, the monomeric units were not defective; i.e., they lacked deletions or other rearrangement ... | 1977 | 198580 |
| characterization of simian virus 40-transformed african green monkey cells (cv-1). ii. semipermissive character for viral replication. | | 1977 | 200829 |
| histone synthesis during infection of monkey kidney cells with simian virus 40. | the synthesis of histones during lytic infection of bsc-1 (african green monkey kidney) cells with sv40 has been investigated. the synthesis of all five classes of histones was stimulated, and all classes appeared to be stimulated to the same extent. the increase in rate of histone synthesis in response to sv40 infection was detectable several hours before sv40 dna synthesis was measureable, and the rate of histone synthesis decreased at a time when sv40 dna synthesis was occuring at a maximal o ... | 1977 | 200893 |
| nonhuman primates: laboratory animals of choice for neurophysiologic studies of sleep. | a systematic study was made of several nonhuman primates to learn more about their relative usefulness for studies of sleep. species studied included the guinea baboon (papio papio), kenya or yellow baboon (p cynocephalus), olive baboon (p anubis), sacred baboon (p hamadryas), vervet monkey (cercopithecus aethiops), rhesus monkey (macaca mulatta), bonnet monkey (m radiata), crab-eating monkey (m fascicularis), patas monkey (erythrocebus patas), chimpanzee (pan troglodytes), mongoose lemur (lemur ... | 1977 | 201798 |
| highly reiterated sequences of simiansimiansimiansimiansimian. | a 172-base pair segment of dna that is repeated several million times in the genome of the african green monkey has been characterized. sequence analysis revealed that the many repeats of this complex unit are not all identical but represent a set of closely related segments: sequence divergence occurs at various positions in the segment in a nonrandom manner. the uncloned segment obtained from monkey dna is compared with a cloned segment of the same dna which was recombined into the genome of s ... | 1978 | 205944 |
| transformation of african green monkey kidney cells with the rf strain of human papovavirus bkv. | | 1978 | 206005 |
| cae i: an endonuclease isolated from the african green monkey with properties indicating site-specific cleavage of homologous and heterologous mammalian dna. | component alpha dna is a highly repetitive sequence that comprises nearly a quarter of the african green monkey (cercopithecus aethiops) genome. a previous microbial restriction enzyme analysis showed that the repeat structure of component alpha dna is based upon a monomeric unit of 176 +/- 4 base-pairs. an endonuclease, provisionally termed case i, has been isolated from african green monkey testes that cleaves component alpha dna into multimeric segments based upon the same repeat periodicity ... | 1978 | 206873 |
| phenotypic complementation of the sv40 tsa mutant defect in viral dna synthesis following microinjection of sv40 t antigen. | african green monkey cells (cv-1p) were microinjected with highly purified sv40 t antigen using protein-loaded red cell ghosts and polyethylene glycol as fusagen. the microinjected cells were infected with a temperature-sensitive mutant of sv40 (tsa209) which is defective in the initiation of viral dna synthesis. using in situ hybridization as an assay method, we found that peg-microinjection of both partially and highly purified t antigen resulted in an increase in the amount of viral dna sequ ... | 1978 | 210962 |
| the process of wound healing of the avascular outer layers of the retina. light- and electron microscopic studies on laser lesions of monkey eyes. | the process of wound healing of the avascular outer layers of the retina is studied on a series of laser lesions of the monkey, cercopithecus aethiops. the extent of the retinal lesions is limited to the pigment epithelium and photoreceptor cells, whereas bruch's membrane and the vascular inner layers of the retina intact. the retinal lesions are not seen to be invaded by cells from the choroid, nor from the retinal vessels. during the first three days after irradiation the pigment epithelial ce ... | 1978 | 211792 |
| the turnover of trnas microinjected into animal cells. | red cell-mediated microinjection has been used to study trna turnover in sv3t3 mouse cells and tc7 cells, an african green monkey kidney line. the turnover of endogenous trna, measured by labeling with 3h-methionine, was first-order with half-lives of approximately one day in sv3t3 and two days in tc7 cells. 32ptrna isolated from e. coli or tc7 cells turned over at the same rate as endogenous trna when injected into either sv3t3 or tc7 cells. this demonstrates that cellular processes, not proper ... | 1978 | 214762 |
| comparative sensitivity of various cell culture systems for isolation of viruses from wastewater and fecal samples. | in efforts to define the most sensitive cell culture systems for recovery of viruses from wastewaters, 181 samples were inoculated in parallel into tube cultures of various cell types and were plaqued in bottle and petri dish cultures of three types of monkey kidney cells. polioviruses were recovered most frequently in the rd line of human rhabdomyosarcoma cells, group a coxsackieviruses in rd and human fetal diploid kidney (hfdk) cells, group b coxsackieviruses in the bgm line of african green ... | 1978 | 215087 |
| reduction of herpes simplex virus-type 2 plaque formation by urea. | the effect of urea on plaque formation by herpes simplex virus type 2 (hsv-2) was examined in two systems at concentrations within or approximating the range found in human urine. approximately 7--10 mg urea/ml, added 2, 4, or 8 hours after infection, reduced plaque formation by 50% in african green monkey kidney cells. the growth of this system was affected slightly by continuous treatment with urea at 7--10 mg/ml. plaque formation was reduced in the monkey kidney system, albeit diminishingly, ... | 1978 | 215721 |
| dnas of simian virus 40 and polyoma direct the synthesis of viral tumor antigens and capsid proteins in xenopus oocytes. | purified simian virus 40 and polyoma dnas injected into nuclei of xenopus oocytes were transcribed and subsequently translated into virus-specific tumor antigens and capsid proteins. simian virus 40 large and small tumor antigens synthesized in the oocytes were indistinguishable, by gel electrophoresis and [35s]methionine-labeled tryptic peptide mapping, from the corresponding polypeptides synthesized in cv-1 african green monkey cells. the synthesis of large simian virus 40 tumor antigen implie ... | 1978 | 216012 |
| evidence for the existence of a single enzyme catalyzing the phosphorylation of choline and ethanolamine in primate lung. | choline kinase (atp:choline phosphotransferase, ec 2.7.1.32) has been isolated and purified 1000-fold from adult african green monkey lung with a yield of 10%. the purified enzyme also phosphorylated ethanolamine (ratio of ethanolamine kinase to choline kinase = 0.30). this ratio remained constant throughout the purification procedure. the km for choline (3.0 - 10(-5) m) was lower than that of ethanolamine (1.2 - 10(-3) m.) choline was also found to inhibit ethanolamine kinase activity by 50% at ... | 1978 | 216394 |
| heterogeneity in the low density lipoproteins of cholesterol fed african green monkeys (cercopithecus aethiops). | the low density lipoproteins of cholesterol-fed african green monkeys were isolated by either preparative ultracentrifugation or agarose column chromatography, then radioiodinated and fractionated by agarose column chromatography. a marked variation was noted in the specific radioactivity of the column fractions regardless of the method used to obtain low density lipoproteins. the radioactivity in each fraction migrated with the beta-globluins, but when the fractions were mixed with carrier plas ... | 1978 | 216395 |
| a simple, rapid syncytial-inhibition test for antibodies to bovine leukemia virus. | cocultivation of equal numbers of cells from a fetal lamb kidney line infected with bovine leukemia virus and african green monkey (vero) cells results in the rapid production of syncytia. the effect was blocked or inhibited by serum containing antibodies to bovine leukemia virus. a serological test based on syncytial inhibition was compared to the agar gel immunodiffusion test and the modified direct complement fixation test for the detection of bovine leukemia virus antibodies in sera from leu ... | 1978 | 217507 |
| some infectious diseases of wild vervet monkeys (cercopithecus aethiops pygerythrus) in south africa. | our surveys indicate that nearly all batches of vervet monkeys arriving at the national institute for virology from various areas of south africa, are infected with foamy viruses and there is evidence that intra-urterine infection also occurs. monkeys from certain areas of south africa are apparently infected with the protozoal parasite entopolypoides macaci in a sub-clinical state which becomes active when the monkeys are splenectomised. serological studies indicate that infections with schisto ... | 1978 | 218005 |
| synthesis of herpes simplex virus dna in isolated chromatin. | herpes simplex virus dna synthesis was studied in isolated chromatin (hsv chromatin) of african green monkey kidney (rc-37) cells after hsv type 1 infection. after optimizing the in vitro system, hsv chromatin was shown to synthesize both viral and cellular dna at ratios identical with those seen in vivo. after 30 min of dna synthesis in vitro, the dna products were identical in size to the prelabeled parental dna. more than 60% of the newly synthesized single-stranded dna fragments sedimented w ... | 1979 | 219885 |
| regulation of simian virus 40 early and late gene transcription without viral dna replication. | primary cultures of african green monkey kidney cells were infected with the simian virus 40 temperature-sensitive mutant tsa58 at the nonpermissive temperature of 41 degrees c for 12 to 20 h. under these conditions, a defective t antigen was produced and no viral dna replication was detected. viral transcription complexes were extracted from infected nuclei using sarkosyl and the nascent chains of rna elongated in vitro. sixty to 70% of the viral rna synthesized in vitro hybridized to late gene ... | 1979 | 221685 |
| the effects of clostridium perfringens enterotoxin on morphology, viability, and macromolecular synthesis in vero cells. | vero (african green monkey kidney) cells grown in tissue culture monolayer were sensitive to clostridium perfringens enterotoxin. within 30 minutes of exposure to the enterotoxin gross morphological damage was observed and within 40 minutes approximately 75% of the cells had detached. nearly half of the cells were nonviable following 35 to 40 minutes incubation with the enterotoxin. doses as low as 0.1 ng caused small but detectable inhibition of plating efficiency of the cells while more than 1 ... | 1979 | 222780 |
| properties of herpes simplex virus dna polymerase and characterization of its associated exonuclease activity. | herpes simplex virus (hsv) dna polymerase was isolated on a large-scale from african green monkey kidney cells infected with hsv type 1 (hsv-1) strain angelotti. after dna-cellulose chromatography the enzyme showed a specific activity of 48,000 units/mg protein. three major single polypeptides with molecular weights of 144,000, 74,000 and 29,000 were copurified with the enzyme activity at the dna-cellulose ste. by its chromatographic behavior and by template studies, the hsv dna polymerase activ ... | 1979 | 223846 |
| interaction between 6/94 virus, a parainfluenza type 1 strain, and mouse macrophages. | the 6/94 virus, a type 1 parainfluenza virus recovered from multiple sclerosis brain cells after lysolecithin-induced fusion of these cells with african green monkey kidney cells (cv-1), has been found to grow in splenic and peritoneal macrophages obtained from outbred and different strains of inbred mice. macrophages from c57bl animals were least susceptible to infection, a resistance apparently partially age related. the virus also has been found to replicate in ic21 cells, a line of simian vi ... | 1979 | 223983 |
| polypeptides of simian rotavirus (sa-11) determined by a continuous polyacrylamide gel electrophoresis method. | simian rotavirus (sa-11) isolated from infected african green monkey kidney cells was separated into two virus fractions in a cscl density gradient and their proteins analysed on a continuous phosphte buffered polyacrylamide gel electrophoresis system. one peak (buoyant density 1.37 g/ml) contained double capsid virus particles which were radioimmunoassay (ria)- and haemagglutinin (ha)- positive and yielded eight polypeptides whose mol. wt. ranged from 48,000 to 128,000. the second peak (buoyant ... | 1979 | 225429 |
| mechanism of host restriction of adenovirus-associated virus replication in african green monkey kidney cells. | human adenovirus (ad) serotypes provide an early factor(s) that is necessary for adenovirus-associated virus (aav) multiplication in human cell lines. however, little, if any, aav production occurs in primary african green monkey kidney (agmk) cells co-infected with aav and a helper human ad (non-permissive infection), unless cells are additionally infected with sv40 (permissive infection). to determine the basis of the host restriction of aav replication in agmk cells, aav dna, rna and protein ... | 1979 | 225435 |
| functional similarity between the early antigens of simian virus 40 and human papovavirus bk. | the functional properties of the early antigens of simian virus 40 (sv40) and human papovavirus bk (bkv) were investigated. infection of african green monkey kidney cells with bkv permitted the bidirectional replication of an early temperature-sensitive mutant (tsa) at a nonpermissive temperature. conceivably, an early gene product (t-antigen) of bkv can substitute functionally for the defective sv40 t-antigen. on the other hand, sv40 dna replication remained undetectable in human embryonic kidn ... | 1979 | 225512 |
| lymphotropic papovaviruses isolated from african green monkey and human cells. | a lymphotropic papovavirus was isolated from a lymphoblastoid cell line of african green monkey (agm) cells which also contained a herpesvirus and a paramyxovirus-like agent. the papovavirus was analyzed by restriction endonuclease cleavage; its biochemical and serological crossreactivity with sv40 and host range have been determined. thus far, only b-lymphoblasts of primate and human origin have been found to be susceptible to infection. although more than 50% of the tested monkey sera were rea ... | 1979 | 226854 |
| intracellular localization of viral polypeptides during simian virus 40 infection. | african green monkey kidney cells infected by simian virus 40 were analyzed by immunofluorescence techniques for the nature and the time course of the appearance of viral polypeptides during infection. reagents used in the study were anti-vpl sera and affinity-purified anti-vpl immunoglobulin g, anti-vp3 sera, antivirus (anti-v) sera, and anti-tumor antigen sera. the results are summarized as follows. (i) three types of staining, nuclear, perinuclear, and perinuclear accompanied by cytoplasmic s ... | 1979 | 228082 |
| alterations in glycosphingolipid patterns in a line of african green monkey kidney cells infected with herpesvirus. | the major glycosphingolipids (gsls) of a line of african green monkey kidney cells (bgm) were characterized as glucosylceramide, lactosylceramide, galactosyl-galactosyl-glucosylceramide, and n-acetylgalactosaminyl-galactosyl-galactosyl-glucosylceramide. neutral gsls accounted for approximately 80% of the total gsls isolated. the predominant gangliosides were n-acetylneuraminyl-galactosyl-glucosylceramide, n-acetylgalactosaminyl-n-acetylneuraminyl-galactosyl- glucosylceramide, and galactosyl-n-ac ... | 1979 | 229262 |
| an effect of cell-culture passage on ultraviolet-enhanced viral reactivation by mammalian cells. | the ability of ultraviolet-irradiated african green monkey kidney cells to reactivate uv-damaged herpes simplex virus was tested over a range of 57 passages (24-81). several types of controls were employed to minimize daily variations. the results indicate that in this system cells demonstrate an increasing efficiency to reactivate uv-damaged virus until approx. passage 60, followed by a decrease in this ability beyond this passage. chromosome counts at selected passages revealed no significant ... | 1979 | 229411 |
| highly repeated dna of the baboon: organization of sequences homologous to highly repeated dna of the african green monkey. | | 1979 | 231675 |
| expression of late viral functions in sv40-infected rat-monkey somatic cell hybrids. | somatic cell hybrids between a rat hepatoma cell line and an sv40-transformed african green monkey kidney cell line (t22 tk-) have been isolated and their response to infection by sv40 virus characterized. the parental rat cells are nonpermissive to productive infection by sv40, while the parental monkey cells, which are t-antigen positive and capsid antigen negative, are permissive to productive infection. hybrid clones, which had segregated monkey chromosomes, were infected with sv40 virus, an ... | 1979 | 232577 |
| antigenic and biologic characteristics of venezuelan encephalitis virus strains including a possible new subtype, isolated from the amazon region of peru in 1971. | nine strains of venezuelan encephalitis (ve) virus isolated from the amazon region of peru in 1971 were identified as antigenic subtype i based on plaque-reduction neutralization tests with four and 20 units of antibody. a tenth strain, 71d1252, was possibly a new subtype, but was related to subtypes i and iii. hemagglutinins of each strain made from infected mouse brains had optimals phs of 6.2 and 6.4. nine strains were pathogenic for adult hamsters and adult mice, but strain 71d1252 inapparen ... | 1975 | 235839 |
| isolation of spotted fever group and wolbachia-like agents from field-collected materials by means of plaque formation in mammalian and mosquito cells. | three isolations from ticks (dermacentor occidentalis) of a rickettsia of the spotted fever group and 5 isolations from chipmunk (eutamias rugicaudus) blood of a wolbachia-like agent were obtained from plaques formed in singh's aedes albopictus (mosquito) and vero (african green monkey kidney) cell cultures. these organisms could not be isolated by injection of the infected ticks or blood into embryonated chicken eggs, guinea pigs, or voles (microtus pennsylvanicus), but fluid cultures of grace' ... | 1975 | 241251 |
| subunit structure of chromatin and the organization of eukaryotic highly repetitive dna: nucleosomal proteins associated with a highly repetitive mammalian dna. | component alpha dna is a homogeneous, highly repetitive fraction that comprises nearly a quarter of the african green monkey (cercopithecus aethiops) genome. by restriction enzyme analysis, it has a repeat periodicity of 176 +/- 4 nucleotide base pairs, corresponding closely with the length of dna contained within a nucleosome. the sequence is organized into large blocks of constitutive heterochromatin. a method is described here for the isolation of intact polynucleosomal arrays containing only ... | 1977 | 269392 |
| density-dependent regulation of growth of bsc-1 cells in cell culture: control of growth by low molecular weight nutrients. | bsc-1 cells, epithelial cells of african green monkey kidney origin, show pronounced density-dependent regulation of growth in cell culture. growth of the cells is rapid to a density of approximately 1.5 x 10(5) cells/per cm(2) in dulbecco-modified eagle's medium supplemented with 10% calf serum. above this "saturation density," growth is much slower. it has been found that the glucose concentration in the culture medium is important in determining the "saturation density." if the glucose concen ... | 1978 | 272650 |
| characterization of cadmium-induced metallothionein in african green monkey kidney cells in vitro. | | 1979 | 299365 |
| propagation of pneumocystis carinii in vero cell culture. | pneumocystis carinii derived from infected murine lung was found to be capable of limited growth in vero african green monkey kidney cell cultures. the observed increase in the number of cyst forms was influenced by the ratio of cysts to host cells in the inocula, duration of passage, and formulation of the cell culture media. maximum growth was achieved by inoculating 1.3 x 10(5) cysts per 75-cm2 flask containing about 2 x 10(7) vero cells (cyst-to-cell ratio=1:154) maintained on minimal essent ... | 1978 | 307535 |
| [assignment of alpha-fuc to1p in man and the chimpanzee and to chromosome 4 in the african green monkey]. | analysis of cellular hybrids confirms the assignment of alpha-l-fucosidase (alpha-fuc) to 1p in man. discordant results are in favour of the following gene order: 1pter (eno-1, alpha-fuc,ak2) pgm1 centromere pep-c but give no information on the relative positions of eno-1,alpha-fuc, and ak2. the assignments of alpha-fuc to chromosome 1 in the chimpanzee and to chromosome 4 in the african green monkey are demonstrated (chromosome nomenclature by finaz et al, 1976). these results confirm the homol ... | 1978 | 308341 |
| experimental palatal candidosis and saliva flow in monkeys. | maxillary acrylic plates, inoculated with candida albicans, were inserted for 3 weeks in 10 monkeys (cercopithecus aethiops) (series i), and reinserted in five of the animals 8 weeks after removal (series ii). to suppress saliva flow oxyphencyclimine was injected intramuscularly (0.125 mg/kg) thrice daily for 3 weeks in six monkeys of series i, while four controls received no drug. in series ii the oxyphencyclimine dose was doubled in three animals, and two controls were sham-treated with sodium ... | 1977 | 402689 |
| the african green monkey as an alternate primate host for studying machupo virus infection. | african green monkeys (cercopithecus aethiops) are highly susceptible to bolivian hemorrhagic fever (bhf). six monkeys were inoculated with 1,000 plague-forming units of machupo virus, the etiologic agent of bhf. they were observed and monitored for clinical signs, body temperature, viremia, hematologic changes, and virus neutralizing antibody. onset of fever, anorexia, and depression was noted on days 3 to 6 postinoculation. these and other signs increased in severity and all monkeys died: 5 of ... | 1977 | 402863 |
| differential habitat utilizati of four cercopithecidae in a kenyan forest. | the presence of papio cynocephalus, cercopithecus mitis, cercopithecus aethiops and colobus angolensis in a small coastal forest is attributed to their differential habitat utilization. data on diet, space-use patterns and polyspecific associations are given. habitat partitioning is found to be resource-related and is accomplished through differential reliance on resources as well as different means of exploiting overlapping resources. the implications of the data for understanding the relations ... | 1977 | 403113 |
| analysis of the alpha-satellite dna from african green monkey cells by restriction nucleases. | by the use of restriction endonucleases the organization of the alpha-satellite dna from african green monkey cells (cercopithecus aethiops) has been analyzed. with endo r-hindiii, endo r-alui and with endo r-ecori at conditions of low salt and high ph (endo r-ecori) all of the satellite was digested while only a part of the satellite was cleaved with endo r-bsu and endo r-ecori under standard conditions. with each of the four nucleases a series of fragments was formed which were multiplies in s ... | 1977 | 404149 |
| assay of escherichia coli heat-labile enterotoxin with vero cells. | the continuous cell line of african green monkey kidney, vero, showed characteristic morphological changes in response to culture filtrates from toxigenic strains of escherichia coli. the response compared favorably with that of y-1 (mouse adrenal) and cho (chinese hamster ovary) cells. vero cells were the simplest and most economical to maintain in the laboratory. | 1977 | 405326 |
| spectral and orientation specificity of single cells in foveal striate cortex of the vervet monkey, cercopithecus aethiops. | 1. the spectral sensitivity, orientation specificity and inhibitory surround of seventy-three cells were studied in the vervet monkey. the eye was in the dark or illuminated with steady white or spectral light. the cells were in the striate cortex corresponding to the foveal representation. nearly all the cells gave on- or on/off-responses.2. more than a third of the cells (41%) responded over a narrow spectral band, in the blue, green, yellow or red section. three quarters of them were orientat ... | 1977 | 406386 |
| respiratory pathogens in non-human primates with special reference to corynebacterium ulcerans. | an investigation of 272 non-human primates (75 macacca cynomolgus, 97 macacca mulatta and 100 cercopithecus aethiops) revealed a high incidence of respiratory disease caused by corynebacterium ulcerans, staphylococci, diplococci and streptococci. escherichia coli was also found as a secondary invader. most of the infections occurred during winter in macaca cynomolgus and were caused by corynebacterium ulcerans and diplococcus pneumoniae. the c. ulcerans strains were phage type vi g. a phage typ ... | 1977 | 407395 |
| application of freeze-dried monkey erythrocytes to measles viral hemagglutination and hemagglutination-inhibition tests. | erthrocytes collected from monkey species including grivet, rhesus, and cynomolgus monkeys were stabilized by fixation with glutaraldehyde of a low concentration and were freeze-dried in vacuo. these freeze-dried erythrocytes were compared with fresh erythrocytes for measles viral hemagglutination and hemagglutination inhibition and could be used in both tests instead of fresh erythrocytes. they maintained their initial appearance and sensitivity to measles viral hemagglutinins after storage at ... | 1977 | 408375 |
| [comparative characteristics of corticosteroid metabolism in lower simians and man]. | corticosteroid metabolism has been investigated in macaca mulatta, papio hamadryas, cercopithecus aethiops, erithrocebus patas and man. similar character of corticosteroid metabolism in monkeys and man was found. significant interspecific differences with respect to quatitative and qualitative parameters of the metabolism of steroid hormones were found. in this respect m. mulatta and p. hamadryas are most similar to man as compared with other monkeys. these two species may be used as an adequate ... | 1977 | 409003 |
| formazan cells in protein and protein-calorie malnutrition. | formazan cells were less numerous in the peripheral blood of vervet monkeys (cercopithecus aethiops) receiving a "o" protein-low calorie diet than in animals fed a full or a protein but not calorie deficient diet. | 1977 | 409269 |
| polymorphonuclear neutrophilic leukocytes in protein deficiency. | the number of white blood cells and of polymorphonuclear leukocytes remained unchanged in vervet monkeys (cercopithecus aethiops) receiving a "o" protein diet. the motility of the polymorphonuclear leukocytes and their phagocytic and killing indices with and without leukokinin stimulation decreased in protein-depleted animals. acid cathepsin decreased, dna relatively increased, and peroxidase, alkaline phosphatase, acid phenylphosphatase, and lysozyme reached higher levels in the polymorphonucle ... | 1977 | 409270 |
| effect of oral estramustine phosphate on pituitary, gonadal, and adrenal function in the green monkey (cercopithecus aethiops sabaeus). | we studied the effects of estramustine phosphate on pituitary, gonadal, and adrenal function in the green monkey. a 2-week course of estramustine phosphate (75 mg per day) reversibly suppressed the luteinizing hormone and testosterone response to luteinizing hormone-releasing hormone but did not affect pituitary-adrenal function assessed by the 11-deoxycorticosterone response to metyrapone. treatment with oral estramustine phosphate also resulted in a significant increase in corticosteroid-bindi ... | 1977 | 409695 |
| evidence for alkali-sensitive linkers in dna of african green monkey kidney cells. | | 1977 | 409957 |
| the susceptibility to praziquantel of schistosoma haematobium in the baboon (papio anubis) and of s. japonicum in the vervet monkey (cercopithecus aethiops). | | 1977 | 410180 |
| aflatoxin b1, a selective inhibitor of dna synthesis in mammalian cells. | aflatoxin b1 in concentrations between 0.01 and 0.1 microgram/ml inhibits dna synthesis in african green monkey cells in culture, but has little effect on rna synthesis and no effect on protein synthesis. the drug even at concentrations up to 1.0 microgram/ml does not interfere with dna repair promoted by ultraviolet irradiation nor does it induce dna repair. the inhibition of dna synthesis attains maximum values 3 h after addition of aflatoxin b1 and is irreversible upon removal of the drug. pr ... | 1977 | 410504 |
| [steroid metabolism in primates. xix. urinary excretion of c21-steroids in cercopithecus aethiops and erythrocebus patas]. | in male cercopithecus aethiops (green monkey; grivet) and erythrocebus patas (dancing red monkey; patas monkey), the pattern of urinary c21 steroids was estimated and compared with those of man, baboon and rhesus monkey. the results indicate diminished 11 beta- and 17 alpha-hydroxylation in steroid biosynthesis as well as diminished delta 4-3-keto reduction and increased 20 beta-reduction in metabolism in these two species. | 1977 | 411649 |
| whole-body cholesterol metabolism in cholesterol-fed african green monkeys with a variable hypercholesterolemic response. | | 1977 | 411859 |
| myocardial lesions in cercopithecus aethiops monkeys. | | 1977 | 412346 |
| ultrastructural study of cell cultures infected with swinepox and orf viruses. | monolayer cells of a porcine kidney cell line were infected with the pp-1 strain of swinepox virus, while secondary or third subcultured monolayer cells of african green monkey kidney were inoculated with the iwate bt-9 strain of orf virus. those infected cells were fixed when cpe became remarkable in the monolayers and examined by electron microscope. in the cytoplasm of cells infected with both viruses, various immature forms of viral particles in different developmental stages were observed. ... | 1977 | 412491 |
| the ciliary body and the suspension of the lens in a monkey (cercopithecus aethiops). a scanning electron microscopic study. | the zonules of zinn and their insertion on the ciliary body and the lens in a monkey (cercopithecus aethiops) have been studied with the scanning electron microscope. the specimens were dissected after drying by the critical point method, and the lens was separated from the ciliary body by simple traction. the pars plana was completely covered by a mat which consisted of meridionally directed zonule-like fibers. most of these fibers inserted at the base of the posterior end of the ciliary proces ... | 1977 | 413329 |
| caliciviruses infecting monkeys and possibly man. | caliciviruses have, for the 1st time, been shown experimentally to infect a primate. twenty-four hours after being inoculated with san miguel sea lion virus (smsv), an african green monkey developed a febrile response and vesicular lesions at injection sites. virus was recovered from lesion material 96 hours later and from the stool at 48 hours. possible human infection with smsv was indicated by serologic evidence. three persons working with 4 distinct serotypes of smsv developed neutralizing a ... | 1978 | 415648 |
| effect of oral pancreatic enzymes on the course of cholera in protein-deficient vervet monkeys. | the effect of pancreatic extract on the course of cholera infection in protein-deficient vervet monkeys (cercopithecus aethiops) was studied. eleven animals were made protein deficient by diet. thier intestinal pancreatic enzyme concentration decreased as they became protein depleted. these animals were then challenged with vibrio cholerae. four control animals given a standard diet were similarly challenged. immediately after challenge, 6 of the protein-deficient monkeys were given a highly pur ... | 1978 | 415928 |
| subunit structure of chromatin and the organization of eukaryotic highly repetitive dna: indications of a phase relation between restriction sites and chromatin subunits in african green monkey and calf nuclei. | | 1977 | 416220 |
| bacterial endocarditis with obstruction of the right atrioventricular orifice and the pulmonary outflow trace in an african monkey (cercopithecus aethiops). | a 7 to 8-year-old male african green monkey (cercopithecus aethiops) was found moribund in his cage. fluid and antibiotic therapy were administered, but the monkey dies 2 hours later. at necropsy, large septic mural thrombi obstructed the right atrioventricular orifice and the pulmonary outflow tract, and smaller septic thrombi were attached to the leaflets of the pulmonary and mitral valves. staphylococci were isolated from the large thrombus occluding the atrioventricular orifice. large absces ... | 1978 | 416298 |
| the prevalence of hepatocystis kochi in african green monkeys. | the prevalence of hepatocystis kochi was studied in 178 african green monkeys from kenya and 61 from ethiopia. examinations of blood smears for gametocytes and examinations of the livers for merocysts were performed to determine the presence of infection in these animals. the overall prevalence of infection was 29%. no significant difference in infection rate was found between monkeys from the different geographic areas or between males and females. | 1978 | 417213 |
| sensory nerve endings in the penis in green monkey (cercopithecus aethiops sabaeus). | the authors examined the sensory innervation of the skin in the penis in green monkey in four adult individuals both in the light and in the elctron microscope. they found 3 kings of nerve endings. the free nerve endings were the most frequently occurring kind of nerve endings in the superficial layers of the corium--altogether 6,444 in number. the second kind of sensory nerve endings is represented by the glomerular endings out of which 96 per cent were found in the papillae. the typical meissn ... | 1977 | 417480 |
| carcinogenic and other adverse effects of procarbazine in nonhuman primates. | the carcinogenic potential of procarbazine is under investigation in three species (macaca mulatta, macaca fascicularis, and cercopithecus aethiops) of nonhuman primates. a total of 55 monkeys have received procarbazine s.c., p.o., or i.p. for periods of up to 12 years. eleven of the 42 monkeys (26%) necropsied thus far have had malignant neoplasms, 6 of which were acute leukemia. two monkeys developed osteogenic sarcomas, two monkeys developed hemangiosarcomas, and a single case of lymphocytic ... | 1978 | 418874 |
| 3,4-dihydroxyphenylacetic acid and homovanillic acid in rat plasma: possible indicators of central dopaminergic activity. | the concentrations of dopamine (da) metabolites (free and conjugated) was measured in plasma and brain regions of rats by the mass spectrometric method of selected ion monitoring. experimental treatments which altered the function of central dopamine neurons also induced concomitant changes in plasma 3,4-dihydroxyphenylacetic acid (dopac) and homovanillic acid (hva). stimulation of the nigrostriatal pathway increased plasma dopac and hva whereas lesion of the pathway decreased plasma metabolites ... | 1979 | 477719 |
| susceptibility of naegleria fowleri to delta 9-tetrahydrocannabinol. | growth of the pathogenic amoeboflagellate naegleria fowleri is inhibited by delta 9-tetrahydrocannabinol (delta 9-thc). delta 9-thc is amoebostatic at 5 to 50 micrograms/ml. delta 9-thc prevents enflagellation and encystment, but does not impair amoeboid movement. calf serum at 10 and 20% (vol/vol) reduces the antiamoeba activity of delta 9-thc. only 1-methoxy delta 8-tetrahydrocannabinol, of 17 cannabinoids tested, failed to inhibit growth of n. fowleri. antinaeglerial activity was not markedly ... | 1979 | 526010 |
| mitochondrial rna and protein synthesis in enucleated african green monkey cells. | | 1978 | 565412 |
| establishment and characterization of a subacute sclerosing panencephalitis (measles) virus persistent infection in bgm cells. | infection of bgm cells with the halle isolate of subacute sclerosing panencephalitis (sspe) gave rise to a persistent infection (bgm/halle), whereas infection of another african green monkey kidney cell line (vero) under identical conditions led to a lytic infection. the bgm/halle cells multiplied more slowly than the non-infected cells (even when the medium was changed daily). under such conditions 10(7) to 10(8) p.f.u./ml/24 h of measles virus was released into the medium. it was established t ... | 1978 | 641529 |
| excision repair of ultraviolet damage in mammalian cells. evidence for two steps in the excision of pyrimidine dimers. | the incidence of pyrimidine dimer formation and the kinetics of dna repair in african green monkey kidney cv-1 cells after ultraviolet (uv) irradiation were studied by measuring survival, t4 endonuclease v-sensitive sites, the fraction of pyrimidine dimers in acid-insoluble dna as determined by thin layer chromatography (tlc), and repair replication. cv-1 cells exhibit a survival curve with extrapolation number n = 7.8 and do = 2.5 j/m2. pyrimidine dimers were lost from acid-insoluble dna more s ... | 1978 | 656544 |
| formation of phenylalanine transfer rna lacking the wye base in vero cells during methionine starvation. | vero, a cell line derived from african green monkey kidney, normally contains a single species of trnaphe (trna2phe), containing a hypermodified base, wye (originally called y), next to the 3' end of the anticodon. when methionine is removed from the growth medium, there appears a new trnaphe species (trna1phe) lacking the wye base and eluting early from reversed phase chromatography columns. its appearance is not due to the cessation of cell growth. addition of methionine to cells containing bo ... | 1978 | 681356 |
| structure and replication of echovirus type 12. 1. analysis of the polypeptides and rna of echovirus 12 particles. | the propagation of echovirus 12 in green monkey kidney (gmk) cells, a continuous cell line, is described. virions and empty capsids are purified to homogeneity by cscl density and by velocity centrifugation. the protein composition of the particles as compared to poliovirus 2 particles is analyzed by dodecyl sulfate/polyacrylamide gel electrophoresis. infectious virions were found to be composed of four different polypeptide chains of molecular weights 33,000, 31,000, 24,000 and 7,000. some addi ... | 1978 | 729593 |
| rapid diagnosis of venezuelan equine encephalomyelitis by fluorescence microscopy. | goat venezuelan equine encephalomyelitis (vee) antiserum and normal serum were conjugated and evaluated for staining sensitivity and specificity. cross-staining with either eastern or western equine encephalomyelitis virus-infected cells did not occur. the baby hamster kidney (bhk-21) cell line when combined with highly specific vee conjugate detected 100 medium suckling mouse intracerebral lethal doses (suckling mouse ld-50/ic) of the 1b subtype of vee virus per milliliter of equine tissue susp ... | 1975 | 803352 |
| thermolabile dna binding proteins from cells infected with a temperature-sensitive mutant of adenovrius defective in viral dna synthesis. | infection of african green monkey kidney cells with type 5 adenovirus leads to the synthesis of two infected, cell-specific proteins with approximate molecular weights of 72,000 and 48,000, that bind specifically to single-stranded but not double-stranded dna. the production of these two proteins was studied after infection with two dna-negative adenovirus mutants belonging to different complementation groups (h5 ts36 and h5 ts 125). both dna binding proteins were detected in cells infected with ... | 1975 | 803568 |
| characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the bgm/mv cell line. | the parameters of a persistent-state measles virus infection in bgm/mv cells were examined. the bgm/mv cell line was established by cocultivation of measles virus-infected primary c3h mouse brain cells with a stable line of african green monkey kidney cells (bgm). initially, a morphologically mixed population of cells existed:bgm-like (epithelioid) and fibroblasts. gradually the fibroblasts were replaced by bgm-like cells, resulting in a morphologically homogeneous population. measles cytopathic ... | 1975 | 803920 |
| evaluation of chromic oxide, glycerol triether, and beta-sitosterol as fecal flow markers in two species of nonhuman primates. | recovery of beta-sitosterol, glycerol triether (1-hexadecyl-2,3-didodecyl glycerol triether), and chromic oxide was studied in african green monkeys and stumptail macaques consuming diets containing 0.75 mg/cal cholesterol and 38% of calories as safflower oil or butter. following oral administration of these compounds, feces were collected daily for 9 days. for all animals, excretion of beta-sitosterol and glycerol triether paralleled one another almost exactly. except for two animals, this was ... | 1975 | 804580 |
| reinfection of vervet monkeys (cercopithecus aethiops) with borrelia hermsii. | two of 4 untreated vervet monkeys infected with borrelia hermsii survived and were resistant to reinfection for at least 1 year. seven of 9 vervets receiving tetracycline could be reinfected after 12 to 36 weeks. immobilizine could be demonstrated for a shorter time in treated than in untreated vervets. | 1975 | 807959 |
| loa loa: experimental infection in two species of african primates. | four species of primates, baboon (papio anubis), patas monkeys (erythrocebus patas), green monkey (cercopithecus aethiops) and chimpanzee (pan troglodytes) were inoculated with third-stage larvae of a human strain of loa loa from cameroon, west african. the baboon and patas monkeys developed patent infections after 135 to 148 days; the green monkeys and chimpanzee did not. in each animal which became patent, microfilaremia rose rapidly to high levels. in the baboon, but not in the patas monkeys, ... | 1975 | 808145 |
| experimental schistosomiasis haematobia in bushbaby (galago crassicaudatus), patas (erythrocebus patas), and grivet (cercopithecus aethiops). | the bushbaby (galago crassicaudatus), patas (erythrocebus patas), and grivet (cercopithecus aethiops) have been evaluated as hosts in experimental schistosomiasis haematobia. individual hosts demonstrated considerable variation in host-parasite relationships. e. patas is suggested as a host for continuing investigations in schistosoma haematobium infections, based upon its moderate susceptibility to infection and a moderate potential for pathological involvement of the urogenital system. | 1975 | 808620 |
| nervous connections between the brain and the pineal gland in the cat (felis catus) and the monkey (cercopithecus aethiops). | silver-impregnated series of cat and monkey (cercopithecus aethiops) brains were studied in an attempt to demonstrate the existence of nervous connections between the brain and the pineal gland via the pineal stalk (central pineal connections). the presence of such connections between both the pineal gland and the habenular area, and between the pineal gland and the posterior commissure was verified in this study. a well defined median nerve tract between the pineal gland and the posterior commi ... | 1975 | 809138 |
| neutralizing antibody and lymphocyte-mediated, colony-forming inhibition responses to measles infection in cercopithecus aethiops monkeys. | the relation between humoral and cellular immune responses to measles virus in cercopithecus aethiops monkedy was examined by in vitro virus neutralization and colony inhibition techniques. both types of specific immune response were maximal two or three weeks after infection and then declined at similar rates for several weeks. however, the humoral response persisted at a low level for at least 44 weeks, whereas the cell-mediated response became undetectable as early as 10 weeks after infection ... | 1975 | 810524 |
| behavioral effects of thymoleptics in vervet monkeys (cercopithecus aethiops). | desmethylimipramine (dmi) exerts diverse quantitative effects on the gross behaviour of monkeys. excitatory as well as sedative effects were observed, which varied according to the general animal temperament. results resembling those obtained with dmi were observed using chlorimipramine and iprindole, although the effects of iprindole were less marked. chlorimipramine also produced immobilization and postural change. the results are discussed in relation to behavioral effects of thymoleptics in ... | 1975 | 810822 |
| [characterization of mycoplasms isolated from imported nonhuman primates (author's transl)]. | mycoplasms were isolated from 35 (16%) of 215 specimens collected from 20 crab-eating monkeys (macaca irus), 9 green monkeys (cercopithecus aethiops) and from 9 common squirrel monkeys (saimiri sciurea). all these animals had been imported from south-east asia, africa and south america being apparently healthy. a total of 38 large and 20 small colony-mycoplasma strains were isolated from the nasal and oral cavity, urethra, vagina and rectal feces. the large colony-mycoplasmas could be differenti ... | 1975 | 811474 |
| morphological changes in the articular cartilage after meniscectomy. an experimental study in the monkey. | the medial meniscus was resected from the right knees of twelve young grivet monkeys that were killed at intervals of twenty-one to 252 days after operation. the knees operated upon and the control knees were investigated radiologically and histologically. degenerative changes occurred in the medial femoral and tibial condyles. at first there was loss of cells from the superficial layer of the articular cartilage, with a marked decrease in the acid mucopolysaccharide content of the matrix. the c ... | 1975 | 811667 |
| the cephalometric analysis of cercopithecus aethiops. | a steiner-based cephalometric study of 46 adult vervet monkeys was undertaken using craniometric points as closely correlated to those in the human as possible. on tracings of each cephalogram 13 measurements were recorded. the results were statistically analysed. whilst the monkey face does vary individually this variation is apparently much less than is that of the human face. it is possible that the positions of the monkey incisor crowns bear a relatively constant relationship to the soft tis ... | 1976 | 813528 |
| immunity in plague: protection induced in cercopithecus aethiops by oral administration of live, attenuated yersinia pestis. | a live, attenuated yersinia pestis vaccine, designated ev76 (paris) f, was pathogenic in cercopithecus aethiops when administered parenterally. although an oral dose of 10(9) colone-forming units of the vaccine was nonfatal to vervets, a transient but severe gastrointestinal disturbance resulted in four of 12 animals. seven immunized vervets were protected against intradermal challenge. the remaining five vervets without serologic reactions and five untreated controls died from typical bubonic ( ... | 1976 | 815445 |
| schistosoma mansoni in baboons. iii. the course and characteristics of infection, with additional observations on immunity. | parasitological, clinical, and histopathological observations on 54 baboons infected with schistosoma mansoni are presented. the baboon and s. mansoni constitute a compatible host-parasite system, evidence by the infectivity of cercariae (98% penetration, 42% adult worm recovery), and the long, fertile life of the worms. baboons tolerated the infection well, with clinical illness a rarity in moderately infected baboons. pathological findings were generally unremarkable. an acute "toxemic" phase ... | 1976 | 816218 |
| rhesus and african green monkey leukocyte response to steroids, antithymocyte globulin, and 60co treatments. | rhesus and african green monkeys were treated with dexamethasome, antithymocyte globulin, 60co irradiation, or combinations of those immunosuppressive agents, and the leukocyte response was followed. the lymphocyte population could be significantly depressed with 60co, but dexamethasone was required to maintain a low lymphocyte count. the ability of the immunosuppressed animals to support the growth of transplanted tumor cells was markedly enhanced by the addition of atg to the treatment schedul ... | 1976 | 817081 |
| development and chimpanzee testing of a vaccine against human hepatitis b. | highly purified hepatitis b virus surface antigen (australia antigen) purified by physical and chemical procedures from infected human plasma was used to prepare hepatitis b vaccine. the purified antigen was treated with formalin and the vaccine was tested exhaustively for safety by ordinary procedures and additionally in marmosets (for live hepatitis b virus). the vaccine was highly potent, inducing antibody in guinea pigs, grivet monkeys, and chimpanzees given three doses of vaccine containing ... | 1976 | 817293 |
| vaccine against human hepatitis b. | a highly purified and inactivated vaccine was made of hepatitis b virus surface antigen. the vaccine was tested exhaustively for safety by ordinary procedures and additionally in chimpanzees and marmosets. it was highly potent and induced antibody in guinea pigs, grivet monkeys, and chimpanzees after three doses of vaccine were given subcutaneously. chimpanzees given three doses of vaccine were protected against challenge with 1,000 chimpanzee-infectious doses of live human hepatitis b virus giv ... | 1976 | 819667 |
| immunologic evidence that the gene for l-gulono-gamma-lactone oxidase is not expressed in animals subject to scurvy. | l-gulono-gamma-lactone oxidase (l-gulono-gamma-lactone:oxygen 2-oxidoreductase, ec 1.1.3.8) is the enzyme that catalyzes the terminal step of l-ascorbic acid biosynthesis in mammalian liver. the absence of the oxidase activity in primates and guinea pigs is the reason why these animals are subject to scurvy, which must be considered an inborn error of metabolism. attempts were made to determine if a protein immunologically crossreactive with l-gulono-gamma-lactone oxidase is present in these ani ... | 1976 | 819930 |
| proline-rich proteins in membranes and contents of monkey (macaca irus and cercopithecus aethiops) parotid zymogen granules. | | 1976 | 821460 |
| representation of fovea in the striate cortex of vervet monkey, cercopithecus aethiops pygerythrus. | | 1976 | 822583 |
| histochemical and biochemical properties of the labial and palatine glands and their secretions in the macaca irus and cercopithecus aethiops monkeys. | the increasing use of non-human primates in experimental studies in dentistry demands detailed knowledge of their basic oral biology. the labial and palatine glands and their secretory products were compared in two commonly used monkey species. the mucous cells of both gland types appeared to elaborate sulphated muco-substances and the serous demilunes of the labial glands neutral mucosubstances. the secretions of these glands, as obtained by in vitro cultivation of glandular tissue were subject ... | 1976 | 822685 |