| the 18 p-syndrome. report of four cases. | four children, two girls and two boys, were found to have a short arm deletion of chromosome no. 18. three of them exhibit a typical dysmorphy of the face showing retraction of the midface, broad-based, flat nose, hypertelorism, epicanthus, "carp mouth", big, protruding, and low set ears, as well as a variable number of turner-like features, failure of growth, mental retardation, and muscular hypotonia. a newly born child, who died at 2 days of age exhibited severe brain defects of holoprosencep ... | 1976 | 954771 |
| [polymorphism of the median crochets of dactylogyrus vastator nyb., 1924 (monogenoidea) from the crucian carp, carassius carassius l]. | there are some variations in the dimensions of anchors depending on the age of the host, localisation and number of parasites. to differentiate such close morphological species as d. vastator and d. crassus it has been suggested to take into account, in addition to taxonomic features, the correlation between the anchor ventroapical length and the bend. | 1976 | 958727 |
| spontaneous ovarian tumors in domestic carp (cyprinus carpio): light and electron microscopy. | massive abdominal enlargement was discovered in 8 adult carp (cyprinus carpio) 4-6 years old, living in breeding ponds of carp fisheries in northern japan. the abdominal enlargements rapidly progressed and the affected fish died within a few months. at necropsy, single or multiple tumors up to 20 cm in diameter were found in the abdominal cavities. ovarian tissue identified within the tumor capsule in 4 fish supported the impression, based on gross anatomic features, that the neoplasm arose in t ... | 1976 | 978770 |
| subunit dissociation in fish hemoglobins. | the tetramer-dimer dissociation equilibria (k 4,2) of several fish hemoglobins have been examined by sedimentation velocity measurements with a scanner-computer system for the ultracentrifuge and by flash photolysis measurements using rapid kinetic methods. samples studied in detail included hemoglobins from a marine teleost, brevoortia tyrannus (common name, menhaden); a fresh water teleost, cyprinus carpio, (common name, carp); and an elasmobranch prionace glauca (common name, blue shark). for ... | 1976 | 1002703 |
| fish swimbladder: an excellent mesodermal inductor in primary embryonic induction. | swimbladder of the crucian carp, carassius auratus, was found to be better as a vegatalizing tissue than other tissues, such as guinea-pig bone marrow, when presumptive ectoderm of triturus gastrulae was used as reacting tissue. swimbladder usually induced assemblies of highly organized mesodermal tissues, such as notochord, somites and pronephric tubules, some of which were covered by mesodermal epithelium without any epidermal covering. a special character of the effect of swimbladder was the ... | 1976 | 1003076 |
| seasonal variations in the histology of the pituitary gland of cirrhinus mrigala (ham.) an indian freshwater major carp, in relation to gonadal activity. | 1. seasonal variations in the histology of the pituitary gland of cirrhinus mrigala (ham.), an indian freshwater major carp, in relation to gonadal activity has been studied during a one year period commencing, january, 1971. 2. the proximal pars distalis undergoes a great deal of seasonal variation in size, weight and nature of pituicytes. 3. the percentage composition of acidophils and cyanophils in the proximal pars distalis show a reciprocal relationship during the year. the acidophils predo ... | 1976 | 1020417 |
| [role of structural factor in the kinetics of free-radical oxidation of lipids in the membranes]. | kinetics of free radical oxidation of lipids (formation of primary molecular products - hydroperoxides, secondary products - carbonyl substances and "fluorescent pigments") was studied in various membrane fragments, which were distinctly differentiated by their fatty acid composition. the non-enzymatic catalysis of lipoperoxidation in these systems was initiated by addition of fe2+ + ascorbic acid. the membrane fragments were isolated from microsomes and mitochondria of rat liver tissue, externa ... | 1976 | 1030897 |
| [changes in the composition of leukocytes of carp after infection with dactylogyrus extensus (monogenoidea, dactylogyridae) in the light of a new classification of formed elements]. | the paper presents new data on the effect of dactylogyrus extensus on the blood of the carp. the data have become available due to a new classification of formed elements worked out by n.t. ivanova. one of characteristic features of dactylogyrosis is eosinophilia. polychromatophil anaemia and anisocytosis were recorded as well. | 1976 | 1035418 |
| amino-acid sequence of parvalbumin from rabbit skeletal muscle. | determination of the complete amino-acid sequence of rabbit skeletal muscle parvalbumin is described. the sequence of 86 of the 109 total residues was determined automatically by sequenator analyses of peptides obtained after cleavage with cnbr or with trypsin. the positions of the remaining 23 residues were determined by subtractive edman degradation of tryptic and chymotryptic peptides. the protein has an acetylated amino terminus. comparison of the rabbit parvalbumin with those from carp, hak ... | 1975 | 1055405 |
| proceedings: cross-reaction of rabbit anti-carp gonadotrophin globulin with gonadotrophic hormone of some teleost fish as tested by immunofluorescence. | | 1975 | 1094085 |
| troponin and parvalbumin calcium binding regions predicted in myosin light chain and t4 lysozyme. | a computer search of available protein sequences and structures suggests that bacteriophage t4 lysozyme contains one region and that rabbit myosin light chains contain three regions similar, and supposedly homologous, to the calcium binding region of carp muscle calcium binding parvalbumin. | 1975 | 1111094 |
| comparative biochemistry of serum albumin. a serum albumin-like protein from carp, cyprinus carpio. | | 1975 | 1122738 |
| significant differences in the degradation of pro-leu-gly-nh2 by human serum and that of other species (38484). | the acyclic c-terminal tripeptide of oxytocin, h-pro-leu-gly-nh-2, is not degraded upon incubation with human (male,female or pregnant female) plasma or serum for 1hr at 37 degrees. however, the sera of other species tested, including rat, chicken and carp, degrade this tripeptide 100%, 4% and 30%, respectively, in 1 hr, as determined by quantitative amino acid analysis of released products. among the species studied there seems to exist a correlation between the anatomic development of the pars ... | 1975 | 1168915 |
| physico-chemical and serological characterization of five rhabdoviruses infecting fish. | viruses isolated from fish with viral haemorrhagic septicaemia (vhs), infectious haematopoietic necrosis (ihn), spring viraemia of carp (svc), swim-bladder inflammation (sbi) and pike fry disease (pfd) have been grown to high titre in fathead minnow cells. while our preparations of the ihn, svc, sbi and pfd viruses showed typical rhabdovirus morphology with bullet-shaped particles and distinct surface projections, the vhs virus preparations had a less typical rhabdovirus morphology but were pleo ... | 1975 | 1170278 |
| fish rhabdoviruses: comparative study of protein structure. | proteins from four fish rhabdoviruses have been studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. the viruses were: trout viral hemorrhagic septicemia (vhs), infectious hematopoietic necrosis virus (ihn), spring viremia virus of carp (svc), and the pike fry rhabdovirus (pfr). for the two salmonid viruses (vhs-ihn), gel electrophoresis indicated the proteins, with molecular weights estimated to be 190,000, 80,000, 38,000, 25,000, and 19,000, respectively. the electrophoretic p ... | 1975 | 1171263 |
| sensitivity of eels (anguilla anguilla) and carp (cyprinus carpio) to type c and e botulinum toxin. | this study has shown for the first time that fish such as eels (anguilla anguilla l.) and carp (cyprinus carpio l.) are sensitive to type e botulinum toxin. with oral administration the mld for eels was 27.500 mouse ld50 per kg body weight, while for carp it was only 236 mouse ld50 per kg. carp were also found to be sensitive to a slight degree to type c botulinum toxin, the mld of the most virulent toxin of this type being 457,000 mouse ld50 per kg with oral administration. eels, however, showe ... | 1975 | 1179868 |
| studies on phagocytosis in fish. i. in vitro uptake and killing of living staphylococcus aureus by peripheral leucocytes of carp (cyprinus carpio). | the clearance and uptake of staphylococcus aureus were studied in vitro in whole blood of immunized and non-immunized carp. bacterial numbers were determined by : (1) counting viable cells in agar plates; (2) counting radioactivity of 14c-labelled bacteria. similar results were obtained by these two methods. neither the bacterial growth nor the ingestion of bacteria into the cells seem to be influences by immunization. in both immunized and non-immunized groups the number of intracellular bacter ... | 1975 | 1193688 |
| multiple sclerosis-associated agent: transmission to animals and some properties of the agent. | in confirmation and extension of observations by carp and his associates, brain tissue and sera from patients with multiple sclerosis (ms) were found to harbor an agent which induces a transitory depression in polymorphonuclear leukocytes (pmn) in mice as well as in rats, hamsters, and guinea pigs. all of eight md brains contained this agent at titers as high as 10(-9)/g of brain tissue. the agent was found in ms sera at titers up to 10(-3)/ml of serum, but its presence depended to some extent o ... | 1975 | 1205617 |
| [intracellular on--off responses of carp retina amacrine cells adapted to the dark]. | dependence of the amplitude and latency of on and off responses of amacrine cells in the isolated dark-adapted carp retina upon the light spot intensity and diameter was studied by intracellular recordings. it is shown that the responses of amacrine cells to onset and offset of light consist of trasient depolarizations with oscillatory potentials and impulses. the latency of the on response decreases and the latency of the off response increases when the light spot intensity and diameter are inc ... | 1975 | 1207845 |
| the use of carp, cyprinus carpio (linnaeus, 1758), instead of rabbit as antibody donor in immunological research on fishes. | antisera produced by carp were tested in the differentiation between two cyprinid fishes, bream and roach, and between the lipovitellins of two grey mullets, mugil cephalus and liza ramada. these antisera were more specific in the recognition of different fish species than those produced by rabbits. an antiserum against carp lipovitellin was produced with male carp as antibody donor. | 1975 | 1211668 |
| [panfuran in aeromonosis (rubella) of carp]. | | 1975 | 1216501 |
| functional morpho-histology of the alimentary canal of an indian frewhwater major carp, labeo rohita (hamilton) during it's different life-history stages. | 1. the alimentary canal of the fry of l. rohita (hamilton) is of the carnivorous type in nature and disposition with a low relative length of the gut (r. l. g.). 2. end-buds are present. the buccopharyngeal region has a simple epithelial surface. mucous cells are small and few throughout the length of the alimentary canal. the presence of these structures in association with a zooplankton feeding habit indicate that the fries are primarily sightfeeders and carnivorous. 3. with increase in age an ... | 1975 | 1217739 |
| on the origin, development and probable functions of taste bud in the lip and bucco-pharyngeal epithelia of an indian freshwater major carp, cirrhinus mrigala (hamilton) in relation to food and feeding habits. | 1. various developmental stages of taste bud have been observed in the fry, fingerling and adult of cirrhinus mrigala (hamilton). 2. the fries are zooplankton-feeders (carnivorous) and sight-feeders. therefore, functional taste buds are absent in the lip and bucco-pharyngeal epithelia. only formative stages of taste bud are present. some epithelial cells aggregate together to form this structure which has no function in gustation. moreover, the fires have low r. l. g. valle (0.71;..1.20) due to ... | 1975 | 1224765 |
| calcium binding sites of rabbit troponin and carp parvalbumin. | | 1975 | 1225590 |
| deletion 11q23-->qter (jacobsen syndrome). report of three new patients. | three unrelated patients with de novo del 11q23-->qter are reported. clinical features included growth and mental retardation, hypotonia, trigonocephaly, facial dysmorphism with hypertelorism, epicanthal folds, abnormally shaped palpebral fissures, eye globe malformations, depressed nasal bridge, "carp-shaped" mouth, highly arched palate, low set and malformed ears. one patient had congenital heart defect, and reduced platelet count. this syndrome, originally reported by jacobsen, is now corrobo ... | 1992 | 1296516 |
| [purification and characterization of hec toxin produced by aeromonas hydrophila]. | an extracellular toxin produced by aeromonas hydrophila from cultured crucian carp with septicemia was detected. the toxin was purified by ammonium sulfate precipitation, deae-cellulose chromatography and sephadex g-100 gel filtration. the factor was a single polypeptide with a molecular weight of 52.5kd determined by sds-page. the heat-stable toxin possesses hemolytic, enterotoxic and cytolytic activities. the hemolytic activity on human erythrocytes was 3.81 x 10(3) hu/mg, cd50 for vero cell w ... | 1992 | 1299032 |
| chemical identification of catfish growth hormone and prolactin. | isolation and primary structure of growth hormone (gh) and prolactin (prl) from the pituitary gland of catfish (ictalurus punctatus) are described. alkaline extract of the pituitary glands was fractionated by gel filtration on sephadex g-75, ion-exchange chromatography on deae-cellulose, and reversed-phase high-performance liquid chromatography on octadecyl silica ods. catfish gh and prl were identified by western blotting with antisera against chum salmon gh and prl. the catfish gh consists of ... | 1992 | 1308206 |
| fish transgene expression by direct injection into fish muscle. | the ability of a promoter sequence to drive expression of a reporter gene can be determined by direct injection of copies of the cloned sequence into fish muscle, followed by biopsy of muscle from the site of injection. we describe a set of experiments in which copies of the constructs fv1 and fv2, both comprising a carp beta-actin promoter sequence spliced to the bacterial reporter gene cat, were injected into the muscle of tilapia fish )oreochromis niloticus) of between 5 and 8 cm body length. ... | 1992 | 1308819 |
| m-phase-specific histone h1 kinase in fish oocytes. purification, components and biochemical properties. | we demonstrate, for the first time in fish, that a ca(2+)-independent and cyclic-nucleotide-independent histone h1 kinase activity oscillates according to the cell cycle of the oocyte, peaking at the first and the second meiotic metaphase with a transient drop between them. the kinase, m-phase-specific histone h1 kinase (m-h1k), was purified from mature carp oocytes by using two exogenous substrates for assaying its activity: histone h1 and a synthetic peptide (sp peptide, kkaakspkkakk) containi ... | 1992 | 1315270 |
| low concentrations of neuroactive peptides modulate cholinergic transmission and cyclic amp levels in helix aspersa. | 1. fmrfamide and the catch relaxing peptide (carp) at 0.01-0.1 nm modulate acetylcholine (ach) induced currents of identified neurons of helix aspersa, but have no direct effect on membrane current and conductivity. 2. both fmrfamide and carp noncompetitively inhibit ach cl- responses while having either no effect or increasing ach na+/k+ response. 3. the inhibitory effect of fmrfamide and carp on the ach cl- response was eliminated following pretreatment with forskolin (20 microm), an activator ... | 1992 | 1338559 |
| electroporation: a method for transferring genes into the gametes of zebrafish (brachydanio rerio), channel catfish (ictalurus punctatus), and common carp (cyprinus carpio). | recombinant plasmids containing the rous sarcoma virus long-terminal repeat (rsvltr) promoter linked to either rainbow trout (oncorhyncus mykiss) growth hormone 1 (rtgh1) or growth hormone 2 (rtgh2) cdna were linearized and introduced into the fertilized eggs of zebrafish (brachydanio rerio), channel catfish (ictalurus punctatus), and common carp (cyprinus carpio) by both electroporation and microinjection. the latter two species had these rainbow trout constructs (rsvltr-rtgh1cdna or rsvltr-rtg ... | 1992 | 1339228 |
| oral absorption of biologically active insulin in common carp (cyprinus carpio l.). | 1. bovine insulin dissolved in 0.05 m deoxycholic acid was absorbed through oral intubation in fish weighing 200-300 g. 2. the peak of absorption appeared in all fish, 30-45 min after intubation and was followed by a gradual decrease. the extent of absorption was extremely variable, with up to a 20-fold difference between individuals. no detectable insulin was found in fish intubated with vehicle. 3. the absorbed hormone retained 17-88% of its lipogenic bioactivity in vitro. the absorbed insulin ... | 1992 | 1347729 |
| immunochemical characterization of rat testicular asparagine synthetase. | we studied immunochemical properties of rat testicular asparagine synthetase. western blot analysis of testis extract with polyclonal antibody raised against purified asparagine synthetase revealed an immunoreactive band at 62 kda. the pancreas, brain, thymus, and spleen also showed 62-kda bands. the intensities of these bands were roughly proportional to the specific activities of the enzyme in these tissues. the antibody showed some degree of cross-reactivity to asparagine synthetases from hum ... | 1992 | 1349469 |
| xenobiotic-metabolizing enzyme systems in test fish--iv. comparative studies of liver microsomal and cytosolic hydrolases. | 1. the initial slopes of the substrate-activity curves of several hydrolases were determined in the microsomal and cytosolic fractions of the liver of several fish recommended by oecd for the regulatory testing of chemicals. 2. inter-species differences ranged within a factor of 7-17 for the esterases and reached a factor of 60 for the amidase. guppy and carp appeared endowed with hydrolase activities which, overall, are much higher than zebra fish, trout and golden orfe. 3. the comparison with ... | 1992 | 1350956 |
| gene expression following transfection of fish cells. | various genes containing different transcriptional regulatory elements (tre) and the bacterial marker gene coding for chloramphenicol acetyl transferase were transfected into several fish cell lines to evaluate the efficiency of expression in comparison with mammalian cells. the cmv and rsv tre were the most efficient non-inducible promoters in directing reporter gene expression. rsv and cmv appeared of similar potency in a stable fish cell line. the human hsp-70 promoter showed high potency in ... | 1992 | 1369156 |
| different sensitivity of the sarcoplasmic reticulum ca(2+)-atpase enzyme to fluorescein-isothiocyanate in rabbit and carp muscles. | 1. carp and rabbit sarcoplasmatic reticulum ca(2+)-atpase enzymes were compared with respect to their sensitivity to fitc labelling. 2. the carp enzyme showed much lower sensitivity to fitc in the ca(2+)-mg2+ activated atpase activity. fifty percent inhibition was observed at 20 microm labelling fitc concentration; in rabbit enzyme this inhibition was already achieved at 2 microm fitc. 3. the tryptic cleavage products of the carp enzyme identified with immunoblot analysis as well as with fitc fl ... | 1992 | 1388116 |
| malathion induced changes in the optomotor behavior of an indian carp, labeo rohita. | | 1992 | 1421850 |
| distribution and coexistence of urotensin i and urotensin ii peptides in the cerebral ganglia of aplysia californica. | urotensin i (ui) and urotensin ii (uii) were demonstrated in the cerebral ganglia of aplysia californica by applying immunocytochemical and radioimmunoassay procedures. sequential analysis of adjacent sections of the cerebral ganglia of aplysia demonstrated that the ui-immunoreactive (ui-ir) neurons of the f cluster of the cerebral ganglia also contained uii immunoreactivity (uii-ir). both ui-ir and uii-ir were also observed in a cuff-like arrangement of fibers surrounding the proximal portion o ... | 1992 | 1437712 |
| retinoic acid enhances the secretion of plasminogen from cultured rat microglia. | to determine the amount of plasminogen in microglial conditioned medium, a highly sensitive and specific enzyme-linked immunosorbent assay (elisa) for rat plasminogen was established. weak cross-reactivity with human serum plasminogen was observed, while no reactivity was detected with frog and carp plasminogen. the specificity of the immunosorbent assay was confirmed by western blotting. the secretion of plasminogen into the microglial culture medium was quantified by using the established elis ... | 1992 | 1459246 |
| interstitial deletion of the long arm of chromosome 11: report of a case and review of the literature. | a 12-month-old female infant with developmental delay, growth retardation, and dysmorphic features including dolichocephaly, telecanthus, ptosis, flat nasal bridge, anteverted nares, high-arched palate, carp-shaped mouth, micro-retrognathia, and low-set and posteriorly rotated ears was found to have an interstitial deletion of chromosome 11 involving bands q14-q22. immunoblot analysis of her fibroblasts revealed a normal amount of mitochondrial acetoacetyl-coenzyme a thiolase, of which gene locu ... | 1992 | 1472705 |
| isolation and characterization of hypothalamic growth-hormone releasing factor from common carp, cyprinus carpio. | a growth hormone-releasing factor (grf)-like peptide was isolated from the hypothalamus of common carp, cyprinus carpio, by acid extraction, gel filtration chromatography, immunoaffinity chromatography using antiserum directed against rat grf, and multiple steps of hplc using octadecyl columns. based on edman degradation and peptide mapping, this teleost grf was established to be a 45-residue peptide with the following primary structure: his-ala-asp-gly-met-phe-asn-lys-ala-tyr-arg-lys-ala-leu-gl ... | 1992 | 1475012 |
| five confirmed human cases of gnathostomiasis nipponica recently found in northern japan. | five confirmed human cases of gnathostomiasis nipponica exhibiting creeping eruption and itching were found sporadically from the autumn of 1991 to the winter of 1992 in the northern region of the mainland of japan. in all cases, a causative gnathostome with 3 transverse rows of hooklets on the head bulb was detected in biopsied skin. the morphological characteristics agreed with the advanced third-stage larvae of gnathostoma nipponicum. within a few weeks before development of symptoms, all pat ... | 1992 | 1491291 |
| [planar charged clusters--structural invariants of the gamma-crystallin family of the crystalline lens: functional role and evolutionary conservatism]. | plane charge clusters from the calf eye lens protein gamma-crystallin are considered. the clusters consist of four to six side chain charged groups with interatomic distances in ionic pairs from 4 to 7 a. the charge clusters appear to decrease the hydrophilic potential of the molecular surface which maintains the transparent refracting lens medium of vertebrates with a very high protein concentration. it is shown that the charge pattern for different gene products of one species is conservative ... | 1992 | 1491683 |
| purification and characterization of cathepsin h from hepatopancreas of carp cyprinus carpio. | 1. cathepsin h was purified about 5400-fold from hepatopancreas of carp (cyprinus carpio) by the method involving ammonium sulfate fractionation, and chromatography on s-sepharose, deae-sephacel, ultrogel aca54, concanavalin a-sepharose 4b and gpc on protein-pak 125. 2. the purified cathepsin h gave a single protein band on analytical-page, but migrated as two bands of 27,000 and 23,000 mol. wt on sds-page. 3. cathepsin h had a ph and temperature optimum of 6.5 and 45 degrees c using arg-mca as ... | 1992 | 1499287 |
| the cellular proliferative response, humoral antibody response, and cross reactivity studies of tetrahymena pyriformis with ichthyophthirius multifiliis in juvenile carp (cyprinus carpio l.). | the immune response of 10-12-week-old juvenile carp to t. pyriformis [ccap 1630/w, 1939 (w)] was investigated. the humoral antibody response following one and two intraperitoneal injections of whole live t. pyriformis (protein concentration of 25 micrograms/g body weight), separated by an interval of 4 weeks, was monitored over 12-16 weeks. peak antibody titres were detected 6 weeks following antigen administration. antibody titres were elevated following the second injection, and relatively hig ... | 1992 | 1505691 |
| demonstration of two forms of vitellogenin in serum of estradiol-17 beta-treated indian major carp, labeo rohita. | vitellogenin (vg) synthesis was induced in the male and non-vitellogenic female rohu, the indian major carp, by estradiol-17 beta(e2) where effect was more in female. a crude preparation of vg was isolated in the second peak after gel filtration on ultrogel aca 34 from the sera of vitellogenic female rohu and e2-treated male and female rohu. estimation of alkali-labile phosphorus was shown to be used as an index of vg. native-page analysis has revealed the presence of two forms of vg (vg1: 430,0 ... | 1992 | 1506024 |
| the role of elongation factors in protein synthesis rate variation in white teleost muscle. | protein synthesis-stimulating activity was assayed in the cytosolic fraction of white muscle from teleost fish (rainbow trout, carp) and of rat liver. in vitro protein synthesis-stimulating activity in the cytosolic fraction is reduced by food deprivation. the addition of elongation factors ef1, ef2, or ef1 + ef2 compensates for the starvation-induced loss of protein synthesis-stimulating activity in trout muscle cytosol. the action of ef2 is stronger than that of ef1 in this respect. however, e ... | 1992 | 1506491 |
| clinostomum complanatum (trematoda: clinostomatidae) in five new fish hosts in japan. | metacercariae of clinostomum complanatum were found in six species of natural freshwater fishes collected at koyama pond in tottori city of tottori prefecture, japan. five of these fish species are reported here as new records for second intermediate hosts of c. complanatum in japan: the silver crucian carp (carassius gibelio langsdorfi), the deepbodied crucian carp (carassius cuvieri), the carp (cyprinus carpio), the topmouth gudgeon (pseudorasbora parva) and the rose bitterling (rhodeus ocelat ... | 1992 | 1512883 |
| ontogenetic and comparative analysis of ldh isozymes in the three species of indian major carp. | expression patterns of lactate dehydrogenase (ldh) isozyme were investigated in embryonic and post embryonic stages of labeo rohita, cirrhinus mrigala and catla catla using starch gel electrophoresis. species specific and differential enzyme locus (gene) expression patterns were found in ldh up to 18th hr of study. the isozyme up to 36th hr after fertilization seemed to be completely active and showed electrophoretic patterns very similar to those of the adults. comparative analysis of the isozy ... | 1992 | 1521871 |
| a monoclonal antibody that recognizes different conformational states of skeletal muscle troponin c and other calcium binding proteins. | skeletal muscle troponin c contains four ca(2+)-binding sites, two with a high affinity for ca2+ that also bind mg2+ competitively (ca2+/mg2+ sites) and two sites of lower affinity that are specific for ca2+. we have characterized a monoclonal antibody (b9d9) that was produced against rabbit skeletal troponin c. the binding of this antibody to rabbit skeletal troponin c is sensitive to the binding of ca2+. increasing the ca2+ concentration produces a decrease in the amount of antibody bound with ... | 1992 | 1527217 |
| sensitivity to maturation-inducing steroids and gonadotropin in the oocytes of blue gourami trichogaster trichopterus (anabantidae, pallas, 1770). | this study examined in vitro the effect of 17 alpha-hydroxyprogesterone (17-p), 17 alpha,20 beta-dihydroxy-4-pregnen-3-one (17,20-p), and human chorionic gonadotropin (hcg) on vitellogenesis and the induction of germinal vesicle breakdown (gvbd) in oocytes of the female trichogaster trichopterus (pallas, 1770) after various in vivo treatments: (a) in nonreproductive condition; (b) in reproductive condition together with males; (c) injected with hcg; (d) injected with extract of carp gonadotropin ... | 1992 | 1577246 |
| structure of the mitochondrial urfa6l gene and trna(lys) gene from carp. | the carp mitochondrial urfa6l gene consists of 165 base pairs. the overall structural organization of the gene is very similar to that of the xenopus urfa6l gene. their nucleotide sequences exhibit 68% homology. the carp urfa6l gene encodes a protein of 54 amino acids. the amino acid composition of the protein is unusual because almost half of the residues consist of 5 hydrophobic amino acids (proline, tryptophan, leucine, isoleucine and tyrosine). a comparison between the amino acid sequences o ... | 1992 | 1580999 |
| study of precorneal tear film thickness and structure by interferometry and confocal microscopy. | past measurements of precorneal tear film thickness in rabbits and humans gave values of 4-7 microns. these have been the accepted values. however, measurements presented in this report are much higher. earlier techniques may have changed film thickness or not have fully included the layer containing mucus. little is known about the mucus component. thickness was measured in freshly killed animals of 10 species using two independent optical techniques. coherent light was reflected from eyes and ... | 1992 | 1582804 |
| [the therapy of swimbladder inflammation (renicola sphaerosporosis) of carp]. | in pond experiments the efficiency of different chemotherapy variants against swimbladder inflammation (sbi) was proved. only by feeding of fumagillin, specifically affecting myxosporidians, the infection of kidney with sphaerospora renicola as well as the force of sbi could be reduced. the chemotherapy with metronidazole remained inefficacious, whereas the permanent feeding with methylene blue prevented only the infection with bothriocephalus acheilognathi and probably lowered insignificantly t ... | 1992 | 1610018 |
| the laboratory maintenance of sanguinicola inermis plehn, 1905 (digenea: sanguinicolidae). | methodology is described for the laboratory maintenance of the life-cycle of sanguinicola inermis, using common carp (cyprinus carpio) as the definitive host and lymnaea peregra as the intermediate host. lymnaea auricularia was also infected but is not considered to be a suitable laboratory host. tench (tinca tinca) were susceptible to infection at high cercarial doses but subsequent infection rates were low. goldfish (carassius auratus) and lymnaea stagnalis were refractory to laboratory infect ... | 1992 | 1614729 |
| [histochemical investigations on the localization of acetylcholinesterase in the kidney of selected vertebrates]. | the light and electron microscopical localization of ache activity in the kidney of selected vertebrates was studied using the method of karnovsky and roots (1964) for light microscopy and the modification of the koelle and friedenwald's technique according to tsuji (1974, 1984) for electron microscopy. ache activity could be demonstrated light microscopically mainly within the glomeruli of some mammals (golden hamster, mouse, rat) and non-mammalian vertebrates (carp, frog). no activity was foun ... | 1992 | 1642103 |
| evidence for glycosylphosphatidylinositol anchoring of intralumenal alkaline phosphatase of the calf intestine. | 1. considerable amounts of intestinal alkaline phosphatase (ap) were found intralumenally in all animal species investigated, i.e. calf, pig, goat, rat, mouse, guinea pig, hen and carp. the ratios between the total activity of ap found intralumenally and the total intestinal activity vary considerably. calves and pigs show the highest, i.e. 0.77 and 0.44, respectively, while rodents have much lower ratios. only 20-34% of the intralumenal alkaline phosphatase (iap) of the calf and pig is soluble ... | 1991 | 1649047 |
| corticostatic peptides. | in the last four years corticostatic (anti-acth) peptides have been isolated from human, rabbit, guinea pig and rat tissues. these peptides do not act via the camp cell signalling system but rather via the inhibition of the binding of acth to its receptor most probably through direct competition with the 14-18 sequence of acth for receptor binding. acth has specific high affinity receptors on adrenal cells but rabbit corticostatin i (csi) has high capacity, low affinity receptors which are compe ... | 1991 | 1659879 |
| [the spherical theory of color perception: its verification by the methods of psychophysics and neurophysiology]. | in the paper data of experimental verification of "spherical theory of perception" (e. n. sokolov) are summed on the material of colour perception study. the results of psychophysical experiments with subjects having different forms of colour perception (normal trichromats, colour anomalies) and neurophysiological experiments on animals (carp) are considered within single psychophysiological system--spherical model of colour discrimination. neuron-like elements of the model reflect the activity ... | 1991 | 1660642 |
| histochemical and immunocytochemical identification of the pituitary cell types in three sciaenid fishes: atlantic croaker (micropogonias undulatus), spotted seatrout (cynoscion nebulosus), and red drum (sciaenops ocellatus). | the adenohypophysial cell types in three species of sciaenid teleosts were identified using a combination of classical histochemical techniques and immunocytochemistry with antisera raised against piscine and human pituitary hormones. in general greater specificity for the different cell types was observed with antisera to the piscine pituitary hormones than with antisera raised against the human hormones. the distribution of the cell types did not differ significantly among the three species, a ... | 1991 | 1667000 |
| regenerative sodium and calcium currents in goldfish retinal ganglion cell somata. | whole-cell patch-clamp recordings were made from isolated goldfish retinal ganglion cell somata. sodium- and calcium-dependent action potentials--blocked with tetrodotoxin (ttx) and co2+ ions, respectively--were evoked under current-clamp. depolarization of these somata under voltage clamp activated distinguishable na+ and ca2+ conductances: the former was permeable to na+, blocked by ttx, impermeable to n-methyl-d-glucamine and tris(hydroxymethyl)aminomethane, and began to activate around -45 m ... | 1991 | 1668863 |
| presence of prolactin receptors in eel liver and carp kidney and growth hormone receptor in eel liver. | 1. 125i-labelled ovine prolactin and bovine growth hormone were used to test for the presence of prolactin and growth hormone receptors in membrane prepared from tissues of the white eel anguilla japonica, the carp ctenopharynogodon idellus and the ricefield eel monopterus albus. 2. high levels of specific 125i-labelled ovine prolactin binding were found in white eel liver membranes and carp kidney membranes. 3. high levels of specific 125i-labelled bovine growth hormone binding were detected in ... | 1991 | 1678335 |
| substrate specificity for myelin basic protein-specific protein methylase i. | the substrate specificity of bovine brain myelin basic protein (mbp)-specific protein methylase i (s-adenosyl-l-methionine:protein-l-arginine n-methyltransferase, ec 2.1.1.23), which methylates arginine residues of protein, has been studied using various mbps, several synthetic peptides and heterogeneous nuclear ribonucleoprotein complex protein (hnrnp). (1) among mbps from different species of brain, the carp mbp was found to be the best substrate for mbp-specific protein methylase i. this high ... | 1990 | 1694691 |
| growth hormone-dependent potentiation of gonadotropin-stimulated steroid production by ovarian follicles of the goldfish. | the possible involvement of growth hormone (gh) in the regulation of ovarian function in the goldfish was investigated by determining the effects of common carp gh on steroid production by vitellogenic and preovulatory ovarian follicles incubated in vitro. carp gh acts in a dose-dependent manner to potentiate the actions of common carp gonadotropin (gth) on the production of 17 beta-estradiol and testosterone by vitellogenic ovarian follicles and the actions of human chorionic gonadotropin (hcg) ... | 1990 | 1697273 |
| development and validation of a competitive enzyme immunoassay for chum salmon prolactin: a comparison to radioimmunoassay. | an enzyme immunoassay (eia), based on a competitive assay system, for the measurement of prolactin (prl) in the pituitary of salmonid fishes and of hormone released in medium from incubated pituitary was developed using a rabbit antiserum to chum salmon prl (prl, a combination of prl i and prl ii). chum salmon prl was coupled to horseradish peroxidase (hrp). the incubation procedure for the antigen-antibody reaction was analogous to that in the radioimmunoassay (ria) for prl. the antibody-bound ... | 1990 | 1705233 |
| presence of four tachykinins in an acid extract of the carp intestinal bulb (cyprinus carpio). | 1. the pharmacological and chemical properties of substance p-like peptides isolated from an acid extract of the carp intestinal bulb were examined using guinea-pig ileum longitudinal smooth muscle. 2. on a sephadex g25 column (3 x 96 cm), smooth muscle contracting material was eluted as two peaks (fraction-1 and fraction-2). the molecular weight of the fraction-1 was estimated to be 2300 and that of the fraction-2 to be 1530. 3. the pharmacological properties of the contracting materials in fra ... | 1990 | 1705490 |
| distribution and migration pathways of hnk-1-immunoreactive neural crest cells in teleost fish embryos. | whole mounts and cross-sections of embryos from three species of teleost fish were immunostained with the hnk-1 monoclonal antibody, which recognizes an epitope on migrating neural crest cells. a similar distribution and migration was found in all three species. the crest cells in the head express the hnk-1 epitope after they have segregated from the neural keel. the truncal neural crest cells begin to express the epitope while they still reside in the dorsal region of the neural keel; this has ... | 1990 | 1706978 |
| tropomyosin from the striated muscles of carp (cyprinus carpio) and of icefish (channichthys rhinoceratus). | tropomyosin of fast-twitch, slow-twitch and cardiac muscles of carp and icefish has been isolated by hydroxyapatite chromatography. the subunit distribution has been investigated by polyacrylamide gel electrophoresis and by peptide mapping. the purified skeletal muscle tropomyosins all belong to the alpha family and differ from higher vertebrate tropomyosin by the lack of beta subunits. specific alpha isotypes are however encountered in fast-twitch fibres (alpha w subunit) and slow-twitch or int ... | 1990 | 1708998 |
| injection of rna from carp retina induces the formation of a membrane potassium channel in xenopus oocytes. | total rna was purified from freshly isolated retinas of adult carp and injected into oocytes of xenopus laevis (stages 5-6). two to six days after injection, depolarizing voltage-clamp steps evoked a slowly activated outward current as large as 3 microa. this current inactivated slowly with a single time constant (tau = 3.1 +/- 0.24 s.e.m., for vm = +30 mv). the current was inhibited by tetraethylammonium (3.8 mm for half-maximal inhibition). in the presence of co2+ (1 mm) or barium methanesulfo ... | 1991 | 1709045 |
| molecular location of a species-specific epitope on the hamster scrapie agent protein. | scrapie is a transmissible neurodegenerative disease of sheep and goats. an abnormal host protein, sp33-37, is the major protein component of the scrapie agent and the only known disease- or agent-specific macromolecule. two monoclonal antibodies (mabs), 4h8 (immunoglobulin g2b [igg2b]) and 6b11 (igg1), produced by immunizing mice with the intact hamster 263k scrapie agent protein, sp33-37ha, were found to have species specificity similar to that reported previously for mab 3f4 (igg2a), which wa ... | 1991 | 1710287 |
| riboflavin carrier protein from carp (c. carpio) eggs: comparison with avian riboflavin carrier protein. | a protein exhibiting immunological cross-reactivity with the chicken egg-white riboflavin carrier protein was detected by radioimmunoassay in the eggs and serum of the fresh water fish cyprinus carpio and subsequently purified to homogeneity by use of affinity chromatography. fish riboflavin carrier protein resembled chicken riboflavin carrier protein with respect to most of its physicochemical characteristics. the major epitopes of chicken riboflavin carrier protein were shown to be conserved i ... | 1991 | 1713763 |
| an enzyme-linked immunosorbent assay for myelin basic protein-specific protein methylase i. | a sandwich enzyme-linked immunosorbent assay (elisa) has been developed to determine myelin basic protein (mbp)-specific protein methylase i. rabbit immunoglobulin anti-bovine mbp-specific protein methylase i, purified by sepharose-a affinity chromatography, was utilized as the primary antibodies, while the same antibodies which had been conjugated to peroxidase were employed as the indicator antibodies. this assay method was about 280 times more sensitive than the conventional trichloracetic ac ... | 1991 | 1715496 |
| plasmodium falciparum reinfection in children from a holoendemic area in relation to seroreactivities against oligopeptides from different malaria antigens. | the rate and densities of plasmodium falciparum reinfections were investigated in children five to 14 years old from one village in tanzania with a high transmission rate. initial parasitemias were eradicated by a curative treatment with quinine, a drug with a short elimination half-life, to minimize the effects of residual drug on reinfection. the seroreactivities to seven oligopeptides, representing t and b cell epitopes from the ring erythrocyte surface antigen (pf155/resa), the clustered arg ... | 1991 | 1718179 |
| glial fibrillary acidic protein in the fish optic nerve. | the intermediate filament glial fibrillary acidic protein (gfap) is the predominant cytoskeletal protein of mature glial cells in the mammalian nervous system. the nervous systems of lower vertebrates, such as fish, have been examined for the presence of gfap and several investigators have shown that goldfish (carassius auratus) brain contains gfap-positive astrocytes. the same studies have demonstrated that, in contrast to the brain, the optic nerve of goldfish did not show any gfap immunoreact ... | 1991 | 1718861 |
| epitopes of the plasmodium falciparum clustered-asparagine-rich protein (carp) recognized by human t-cells and antibodies. | linear b- and t-cell epitopes have been identified in the plasmodium falciparum clustered-asparagine-rich-protein (carp). twenty-six synthetic peptides, 15-25 amino acids in length, were assayed for their ability to stimulate purified, human t-cells primed to p.falciparum by natural infection to proliferate and/or secrete gamma-interferon (ifn gamma). the plasma of malaria exposed individuals were tested for antibody reactivity with peptides coupled to bovine serum albumin in a semiquantitative ... | 1991 | 1725821 |
| purification and characterization of maturation-promoting factor in fish. | maturation-promoting factor (mpf) activity has been demonstrated for the first time in fish oocytes. we purified mpf from a 100,000g supernatant of crushed, naturally spawned carp oocytes using four chromatography columns: q-sepharose fast-flow, p13suc1-affinity sepharose, mono s, and superose 12. the final preparation was purified over 1000-fold with a recovery of about 1%. on superose 12, mpf eluted as a single peak with an apparent molecular weight of 100 kda. sds-page analysis of the active ... | 1992 | 1728595 |
| purification and characterization of a calsequestrin-like calcium-binding protein from carp (cyprinus carpio) sarcoplasmic reticulum. | 1. a calsequestrin-like calcium-binding protein was purified from carp sarcoplasmic reticulum by column chromatographies using deae-cellulose and butyl-toyopearl 650s. 2. the mol. wt was estimated to be 50 kda, which was larger than that of rabbit calsequestrin (42 kda). 3. carp calsequestrin-like protein bound ca2+ with a higher affinity (apparent kd = 400 microm) and lower capacity (25 mol/mol) compared with rabbit calsequestrin (1 mm and 40-50 mol/mol, respectively). 4. anti-carp calsequestri ... | 1991 | 1769203 |
| effect of proteases on the activity of enolase from muscle of carp (cyprinus carpio) and pig. | the treatment of enolase from pig and carp (cyprinus carpio) with proteases resulted in a decrease of enzymatic activity, which depended on the kind of protease used. the most active were trypsin and subtilisin. substrate and magnesium ions protected enolase against inactivation. the enolase from pig muscle was much more resistant to protease action than this enzyme from carp muscle. some differences in the structure between the two enolases are suggested. | 1991 | 1796695 |
| development of khawia sinensis hsü, 1935 (cestoda: caryophyllidea) in the fish host. | the development of the tapeworm khawia sinensis has been observed up to the stage of sexually mature parasites releasing eggs in an experimentally infected definitive host (cyprinus carpio) at 15-16 degrees c. juvenile, maturing, adult and gravid tapeworms were found 2-12, 19-24, 36-62, and 78-91 days post infection, respectively. no apparent size difference between tapeworms from carp kept at 20-22 degrees c, examined 1-16 days post infection, and those from 15-16 degrees c was observed. in nat ... | 1991 | 1808032 |
| [elaboration of a dried-salted and smoked product using aquaculture species]. | the purpose of this work was to adapt the procedure developed at the instituto nacional de la nutrición salvador zubirán (innsz) for the conservation of the sardine to aquaculture species such as carp (ciprinus carpio) and tilapia (tilapia sp). the mixtures were designed on the basis of the chemical score method. the preparation of the patties included different fish presentations, and two methods were evaluated in order to obtain fish pulp. in the formulation of the final product, defatted soy, ... | 1991 | 1824516 |
| [trematodiases in tataria]. | high opisthorchiasis morbidity among local population and domestic animals was recorded in 1966 in alekseevskiĭ district of tataria. repeated screenings performed in 1989 revealed besides the above disease cases of pseudoamphistomiasis. 183 subjects have been examined, 90 of them (40.9%) revealed hepatobiliary system damages. bile analysis performed in 61 patients revealed lambliasis in 1 case, opisthorchiasis in 3 cases and pseudoamphistomiasis in 31 cases. metacercaria of the above trematode w ... | 1991 | 1829788 |
| carp erythrodermatitis (ce) due to an aeromonas hydrophila infection. casuistic and experimental results. | in november 1987 high losses of carp (cyprinus carpio) with the main symptom of skin ulcera were observed in a farm in northern greece. sixty-six isolates of bacteria, characterized mainly as aeromonas hydrophila or pseudomonas spp. could be isolated from lesions of diseased fish. transmission experiments with these isolates using mirror carp showed that aeromonas hydrophila strains induced identical clinical and pathological pictures after intra- or subcutaneous injection. extracts of these aer ... | 1991 | 1830440 |
| identification of immunoreactive atrial natriuretic peptide in bulbus arteriosus of freshwater teleostean fish. | 1. the presence and partial characterization of immunoreactive atrial natriuretic peptide (ir-anp) in the bulbus arteriosus were investigated using high performance liquid chromatography (hplc) and radioimmunoassay (ria) in several freshwater teleostean fish (catfish, silver crusian carp, snakehead, and rice eel). 2. the level of ir-anp in the bulbus arteriosus was comparable with that in the atria at about 5-15 pg/mg, 6-13 pg/mg wet wt, respectively, but was 2-4-fold higher than that in the ven ... | 1991 | 1839978 |
| studies on rod horizontal cell s-potential in dependence of the dark/light adapted state: a comparative study in cyprinus carpio and scyliorhinus canicula retinas. | changes in light sensitivity, response time-course and electrical coupling of rod driven horizontal cells (rhc) were examined in the dark and light adapted states by intracellular recording. comparative analyses were made of two fishes from different photic environments. rhc of cyprinus carpio and external horizontal cells (ehc) of scyliorhinus canicula retinas showed similar changes of their electrical responses to light stimuli when the dark-adapted retina was light-adapted: a decrease in sens ... | 1991 | 1843752 |
| calcium channels from cyprinus carpio skeletal muscle. | the complete amino acid sequence of the l-type calcium channel alpha 1 subunit from the carp (cyprinus carpio) white skeletal muscle was deduced by cdna cloning and sequence analysis. the open reading frame encodes 1852 amino acids (mr 210,060). a 155-amino acid cooh-terminal sequence (after the fourth internal repeat) is evolutionarily preserved (90% homology) and may represent an important functional domain of l-type calcium channels. the photolabeled, membrane-bound, and purified carp alpha 1 ... | 1991 | 1846962 |
| [the immunological status of cyprinus carpio l. in experimental mycotoxicosis]. | some humoral factors of nonspecific resistance have been determined in carp yearlings in 28 days after intraperitoneal administration of fungi culture filtrate (i), conidia (ii), micellar extract (iii) of the fungus aspergillus fumigatus fres., physiological solution (control). the toxicogenic and immunogenic effect of the fungus has been shown in i and ii variants of the experiment respectively. the injection of fungus micella did not have any influence on the studied factors. the account of sp ... | 1991 | 1859857 |
| absorption of bioactive human growth hormone after oral administration in the common carp (cyprinus carpio) and its enhancement by deoxycholate. | recombinant human growth hormone was administered orally to carp and serum levels of absorbed bioactive hormone were investigated using a highly sensitive nb2 rat lymphoma cell bioassay and radioimmunoassay. serum levels of bioactive hgh reached maximum values 30 min after oral intubation and then gradually decreased. co-administration of the hormone with deoxycholate to fasted carp resulted in up to a 1000-fold increase in absorption compared to aqueous solutions of the hormone, but had no effe ... | 1991 | 1869695 |
| effects of organophosphorus insecticides: effects of trichlorfon and dichlorvos on the immune response of carp (cyprinus carpio). iii. in vitro effects on lymphocyte proliferation and phagocytosis and in vivo effects on humoral response. | cells isolated from the pronephros of carp were incubated in vitro with the organophosphorus insecticide trichlorfon or dichlorvos, each of which is used in aquaculture to eliminate fish ectoparasites. dose-dependent suppressive effects were observed in assays for lymphocyte proliferation and myeloid cell respiratory burst activities. dichlorvos given by bath in vivo did not affect antibody production against yersinia ruckeri even if the spleen and kidney were obviously contaminated according to ... | 1991 | 1914997 |
| [contamination of fish tissue cell cultures by mycoplasma]. | the inoculated and primary cell cultures of fish (carp, salmon, and sturgeon) have been studied. acholeplasma typed as a. laidlawii in terms of its biochemical properties shown in inhibited metabolism has been isolated from 19 samples. the authors consider the source of acholeplasma-induced contamination of the cell cultures under study. | 1991 | 1927041 |
| goussia carpelli (apicomplexa, eimeriorina) from cyprinid fish: field observations and infection experiments. | intestinal tissue samples of 9 cyprinid and gobiid fish species from several hatcheries and ponds in south bohemia were found to harbour goussia carpelli-like coccidian oocysts. furthermore, laboratory transmission of goussia carpelli, obtained from common carp, was attempted to 9 cyprinid fish species, both laboratory reared and obtained from pond environments. infection experiments, carried out by fecal contamination (direct transmission) and by using tubificid oligochaetes as paratenic hosts ... | 1991 | 1928799 |
| insulin-like growth factor i-like immunoreactivity in serum and tissues of the tilapia, oreochromis mossambicus. | tilapia serum was acidified with 0.5 m hcl and then chromatographed on an octadecasily-silica column. after washing with 4% acetic acid, the column was eluted with methanol. the eluate was evaporated to dryness. the sample cross-reacted in a human insulin-like growth factor i (igf-i) radioimmunoassay, suggesting immunochemical similarity to human igf-i. igf-i-like immunoreactivity was present at high levels in tilapia liver. other tissues containing igf-i-like immunoreactivity included the gonad ... | 1991 | 1930252 |
| on the dissemination of two far east monogenean parasites (dactylogyrus achmerovi gussev, 1955 and gyrodactylus kherulensis ergens, 1974, of carp (cyprinus carpio l.)) in czechoslovakia. | | 1991 | 1937271 |
| early development of khawia sinensis hsü, 1935 (cestoda: caryophyllidea), a carp parasite. | the development of the tapeworm khawia sinensis parasitizing carp (cyprinus carpio l.) has been studied from the release of cestode eggs into water to the formation of infective procercoids in the intermediate host under laboratory conditions. the rate of embryonal development is greatly influenced by the water temperature. while at 5 degrees c the development of oncospheres was not recorded, at 10-12 degrees c, 15 degrees c, 20-22 degrees c, and 23-24 degrees c oncospheres were fully formed aft ... | 1991 | 1937272 |
| [characteristics of adenosine triphosphatase activity in carp erythrocytes treated with saponin]. | the activities of ca-atpase and na,k-atpase in saponin-treated erythrocytes of man, rat and carp were compared. it was shown that at free calcium concentrations lower than 1 microm the activity of ca-atpase in carp erythrocytes was by one order of magnitude lower than in rat erythrocytes and 3-4 times lower than in human red blood cells. at [ca2+] = 0.4 microm the activities of na,k-atpase in all species under study were essentially the same. the increase in ca2+ concentration up to 1 microm res ... | 1990 | 1963091 |
| isoelectric analysis of 3h-pargyline-labelled monoamine oxidase in rat and carp. | 1. after selective binding of [3h]pargyline to either monoamine oxidase (mao) a or mao b in the rat liver, mao b alone in the rat brain and mao in carp brain and liver, molecular weight and isoelectric points (pi) of these mao were determined by sodium dodecyl sulphate (sds)-polyacrylamide gel electrophoresis and isoelectric focusing and results obtained were compared. 2. for all tissues tested, sds-polyacrylamide gel electrophoresis of [3h]pargyline-bound samples revealed a labelled protein ban ... | 1990 | 1980888 |
| the crystal structure of silver carp insulin at medium resolution. | it is an important way of surveying the structure-function relationship of insulin to study insulins from different species. based on the structure model of an orthorhombic crystal obtained by the molecular replacement method, the crystallographic refinement of a hexamer of silver carp insulin in an asymmetric unit has been carried out with 2.8 a resolution data using the restrained least-squares method. the comparisons of insulin structures have shown that the six silver carp insulin molecules ... | 1991 | 2021422 |
| purification and properties of neutral beta-n-acetylglucosaminidase from carp blood. | a neutral beta-n-acetylglucosaminidase has been purified to homogeneity from carp blood by a seven-step procedure. it was localized in the cytosol of red blood cells. the purified enzyme was specific to beta-n-acetylglucosaminide and inactive to beta-n-acetylgalactosaminide. it was competitively inhibited by free n-acetylglucosamine, but not by free n-acetylgalactosamine. the optimum ph of the enzyme was 6.5, with a stable ph range of 7.0 to 11.0. the enzyme showed greater heat-lability and anod ... | 1991 | 2043684 |
| development of a homologous radioimmunoassay for eel prolactin. | a highly specific and homologous radioimmunoassay (ria) for the measurement of prolactin (prl) in the plasma and the pituitary of the eel was developed using a rabbit antiserum to eel prl. prl was purified from the pituitary of japanese eel (anguilla japonica). pituitary extracts and plasma from the japanese and european eels exhibited displacement curves parallel to the eel prl standard. plasma and pituitary extracts from chum salmon, rainbow trout, japanese charr, tilapia, goldfish, and carp, ... | 1991 | 2055440 |
| growth of organs and tissues in carp (cyprinus carpio l.) larvae. | growth of some internal organs and tissues was studied in carp (cyprinus carpio l.) in order to provide baseline information on the growth process during the first posthatching stages of fish, and to compare the trend observed in fish to that known in other animals. growth of organs and tissues (liver, pancreas, trunk muscles, digestive tract, eyes, nervous system) was studied in rapidly growing carp (cyprinus carpio l.) larvae by means of a quantitative histological method. mean body weight of ... | 1990 | 2074119 |
| conformational comparisons among homologous bony fish 5s ribosomal rnas. | the reversible thermal melting of homologous 5s ribosomal rnas of three bony fishes, rainbow trout (salmo gairdneri), carp (cyprinus carpio), and dog salmon (onchorhynchus keta), were compared in aqueous salts (sodium chloride and/or divalent metal chlorides) by means of differential optical absorption at 260 and 280 nm, and c.d. at 265 nm. the melting parameters of the multiphasic melting process were conserved among the homologous rnas, and it was shown that they did not depend on the methods ... | 1990 | 2083239 |