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distinct genetic lineages of bactrocera caudata (insecta: tephritidae) revealed by coi and 16s dna sequences.the fruit fly bactrocera caudata is a pest species of economic importance in asia. its larvae feed on the flowers of cucurbitaceae such as cucurbita moschata. to-date it is distinguished from related species based on morphological characters. specimens of b. caudata from peninsular malaysia and indonesia (bali and lombok) were analysed using the partial dna sequences of cytochrome c oxidase subunit i (coi) and 16s rrna genes. both gene sequences revealed that b. caudata from peninsular malaysia ...201222615962
olfactory cues from plants infected by powdery mildew guide foraging by a mycophagous ladybird beetle.powdery mildews (erysiphales) are economically important plant pathogens that attack many agricultural crops. conventional management strategies involving fungicide application face challenges, including the evolution of resistance and concerns over impacts on non-target organisms, that call for investigation of more sustainable alternatives. mycophagous ladybird beetles (coleoptera: coccinellidae) feed on powdery mildew and have considerable potential as biological control agents; however, the ...201121876772
silicon efflux transporters isolated from two pumpkin cultivars contrasting in si uptake.the accumulation of silicon (si) differs greatly with plant species and cultivars due to different ability of the roots to take up si. in si accumulating plants such as rice, barley and maize, si uptake is mediated by the influx (lsi1) and efflux (lsi2) transporters. here we report isolation and functional analysis of two si efflux transporters (cmlsi2-1 and cmlsi2-2) from two pumpkin (cucurbita moschata duch.) cultivars contrasting in si uptake. these cultivars are used for rootstocks of bloom ...201121617377
nutritional, sensory and physical analysis of pumpkin flour incorporated into weaning mix.the objective of this study was to develop a cereal-pulse complementary food fortified with different concentrations of pumpkin powder (cucurbita moschata), and to analyse its sensory and physic-chemical parameters. fresh pumpkins(cucurbita moschata) were procured from the market and dehydrated and powdered in the laboratory. sorghum (sorghum vulgare) and whole green gram (vigna radiate) were germinated, dried, pulverised and combined with powdered rice (oryza sativa) in the ratio of 2:1:1. pump ...201022691991
new phenolic glycosides from the seeds of cucurbita moschata.two new phenolic glycosides were isolated from the seeds of cucurbita moschata. their structures were elucidated as (2-hydroxy)phenylcarbinyl 5-o-benzoyl-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (1) and 4-beta-d-(glucopyranosyl hydroxymethyl)phenyl 5-o-benzoyl-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (2) on the basis of spectroscopic analysis and chemical evidence.200920183300
structures of new phenolic glycosides from the seeds of cucurbita moschata.a new phenolic glycoside and three known compounds were isolated from the seeds of cucurbita moschata. the structures of the new compound was elucidated as phenylcarbinyl 5-o-(4-hydroxy)benzoyl-beta-d-apiofuranosyl (1-->2)-beta-d-glucopyranoside on the basis of spectroscopic analysis and chemical evidence. three known compounds were identified as 1-o-benzyl[5-o-benzoyl-beta-d-apiofuranosyl(1-->2)]-beta-d-glucopyranoside 2, cucurbitosides c 3 and a 4, by comparison of the spectral data with repor ...200919475995
[effects of nacl stress on free polyamines content and reactive oxygen species level in pumpkin roots].taking cucurbita moschata duch hybrid 360-3 x 112-2 and c. ficifolia bouche as test materials, the effects of nacl stress on their plant growth and the o2*- production rate and h2o2 and free polyamines (pas) contents in their roots were studied with hydroponic culture. the results showed that after 10 d nacl stress, the plant growth of the two pumpkin varieties was strongly inhibited, compared with the control, and c. ficifolia was more injured than hybrid 360-3 x 112-2. under nacl stress, the r ...200819102314
location of caspase 3-like protease in the development of sieve element and tracheary element of stem in cucurbita moschata.the casepase is considered to regulate the process of programmed cell death in the development of organisms. in this study, caspase 3-like protease was detected by immunohistochemistry and immunoelectron microscopy during the development of sieve element and tracheary element of stem in cucurbita moschata duch. antibody with brown color (under light microscopy) and gold particles (under transmission electron microscopy) for detecting caspase 3-like protease was mainly displayed in inner phloem, ...200819093968
excellent combination of counter-current chromatography and preparative high-performance liquid chromatography to separate galactolipids from pumpkin.galactolipids in the fruits of cucurbita moschata (pumpkin) could not be completely separated by high-performance liquid chromatography (hplc). preparative hplc was not available for preparing major galactolipid monomers in pumpkin. in the present paper, a combination of high-speed counter-current chromatography (hsccc) and preparative hplc was used for preparing the galactolipids. a fraction containing galactolipids (fr60) from the purification of the n-butanol extract of pumpkin by macro-porou ...200919081570
ssr-based genetic linkage map of cucurbita moschata and its synteny with cucurbita pepo.the first ssr-based genetic linkage map of cucurbita moschata was created by integrating the maps of two f2 populations with one common parent developed from the crosses waltham butternut (wb) x nigerian local (nl) and zhou (a hull-less type) x wb. the integrated c. moschata map comprises 205 ssr markers and two morphological traits (gr and n). the map is composed of 27 linkage groups with a marker density of 7 cm. comparing the c. moschata map with the published cucurbita pepo map, we found a h ...200818956020
[effects of nacl stress on cation contents in different pumpkin cultivars' seedlings].with the seedlings of 19 pumpkin cultivars as test materials, this paper studied the variations of na+, k+, ca2+, na+/k+, na+/ca2+, sn+, k+ and sna+, ca2+ in their shoots and roots under the stress of 300 mmol nacl x l(-1). the results showed that after an 8-day exposure to 300 mmol nacl x l(-1), the na+ content in the seedlings increased significantly while the k+ content decreased, resulting in the brokenness of ion balance. the root na+ content, shoot na+/k+ and na+/ca2+ ratios, and sna+, k+ ...200818533527
copper and lead levels in two popular leafy vegetables grown around morogoro municipality, tanzania.a study was carried out to determine the levels of two heavy metals, lead (pb) and copper (cu), in two popular leafy vegetables grown around morogoro municipality in tanzania. vegetable samples of pumpkin leaves (cucurbita moschata) and chinese cabbage (brassica chinensis) were collected from three sites and analysed for their concentrations of the two metals using an atomic absorption spectrophotometer. the three sites, namely mazimbu, kihonda and towelo are located within a 10 km perimeter. th ...200617058799
[effects of nacl stress on cation contents in seedlings of two pumpkin varieties].effects of different concentration nacl stress on cation contents of pumpkin cultivars (cucurbita ficifolia and cucurbita moschata) usually used as rootstocks of cucumber were studied. the results showed that, in both cultivars treated with 150, 300 and 500 mmol/l nacl for 7 d, increasing salinity caused in increasing retardation of shoot and root growth, and reduction of shoot relative water content (table 1). with increasing nacl concentration, na(+) content increased while k(+) content decrea ...200616477137
[allelopathic effects of cultured cucurbita moschata root exudates].by using the techniques of tissue culture, bio-assay and laboratory analysis, this paper studied the effects of the allelopathic chemicals from pumpkin (cucurbita moschata) roots on the seed germination and seedling growth of pumpkin, wheat (triticum aestivum), and radish (raphanus sativus). the pumpkin root was cultured on a sterile b5 media, and the concentrations of macro- and microelements, organic supplements and hormones in the media were adjusted by using an orthogonal design. after cultu ...200516011180
[effects of root exudates of squash grafted with cucumber shoot on seed germination].cucumber (cucumis statirus l.) is commonly cultivated by grafting on squash (cucurbita moschata) in commercial production. the effects of root exudates of squash grafted with cucumber on seed germination rate of cucumber and squash were tested. in this study, the exudates of cucumber-grafted squash roots were continuously collected with xad-4 resin, and were further diluted with distilled water to make 5%, 10%, 20% and 40% concentrations. cucumber and squash seeds were used for bioassay. the see ...200515840942
new phenolic glycosides from the seeds of cucurbita moschata.five new phenolic glycosides, cucurbitosides a-e (1-5), were isolated from the seeds of cucurbita moschata. their structures were elucidated as 2-(4-hydroxy)phenylethanol 4-o-(5-o-benzoyl)-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (1), 2-(4-hydroxyphenyl)ethanol 4-o-[5-o-(4-hydroxy)benzoyl]-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (2), 4-hydroxybenzyl alcohol 4-o-(5-o-benzoyl)-beta-d-apiofuranosyl(1-->2)-beta-d-glucopyranoside (3), 4-hydroxybenzyl alcohol 4-o-[5-o-(4-hydroxy)b ...200515684523
[immunocytological localization of iaa in the parenchyma cell and vascular elements in the graft union of cucurbita pepo/cucurbita moschata at the early developmental stage].immuno-gold localization of iaa in cells of the graft union in the explant internode graft of cucurbita pepo/cucurbita moschata were investigated with electron microscopy. in parenchyma cells near the graft union, the gold particles were mainly accumulated in nucleus, plastid and endoplasmic reticulum, while no gold particles was detected in golgi body, mitochondrion, cell wall and vacuoles. in the differentiating xylem element, the gold particles were labeled in secondary wall and cytoplasm. in ...200012548981
germanium does not substitute for boron in cross-linking of rhamnogalacturonan ii in pumpkin cell walls.boron (b)-deficient pumpkin (cucurbita moschata duchesne) plants exhibit reduced growth, and their tissues are brittle. the leaf cell walls of these plants contain less than one-half the amount of borate cross-linked rhamnogalacturonan ii (rg-ii) dimer than normal plants. supplying germanium (ge), which has been reported to substitute for b, to b-deficient plants does not restore growth or reduce tissue brittleness. nevertheless, the leaf cell walls of the ge-treated plants accumulated considera ...200212481079
13-hydroxy-9z,11e,15e-octadecatrienoic acid from the leaves of cucurbita moschata.a new unsaturated hydroxy fatty acid was isolated from the leaves of cucurbita moschata through repeated silica gel column chromatography and chemical methods. the structure of the new fatty acid was determined as 13-hydroxy-9,11,15-octadecatrienoic acid on the basis of several spectral data including 2d-nmr. the stererostructures of double bonds were determined to be 9z, 11e and 15e by coupling patterns of related proton signals in the 1h-nmr and noesy experiments.200212214851
carotenoid composition and vitamin a value of an argentinian squash (cucurbita moschata).the carotenoid composition of butternut squash (cucurbita moschata) cultivated in the province of santiago del estero, argentina, was determined. the main carotenoids isolated were identified as beta-carotene (beta,beta-carotene), alpha-carotene (beta,epsilon-carotene), and lutein (beta,epsilon-carotene-3,3'-diol) and the minor carotenoids, as phytofluene (7,8,11,12,7',8'-hexahydro-psi,psi-carotene), zeta-carotene (7,8,7',8'-tetrahydro-psi,psi-carotene), neurosporene (7,8-dihydro-psi,psi-caroten ...200112012567
kinetic mechanism and order of substrate binding for sn-glycerol-3-phosphate acyltransferase from squash (cucurbita moschata).sn-glycerol-3-phosphate acyltransferase (g3pat, ec 2.3.1.15), a component of glycerolipid biosynthesis, is an important enzyme in chilling sensitivity in plants. the three-dimensional structure of the enzyme from squash (cucurbita moschata), without bound substrate, has been determined [turnbull et al. (2001) acta crystallogr. d 57, 451-453; turnbull et al. (2001) structure 9, 347-353]. here we report the kinetic mechanism of plastidial g3pat from squash and the order of substrate binding using ...200211943166
morphological, biochemical and molecular characterization of herpetomonas samuelpessoai camargoi n. subsp., a trypanosomatid isolated from the flower of the squash cucurbita moschata.we report the morphological, biochemical and molecular characteristics of a trypanosomatid isolated from the flower of cucurbita moschata. although the trypanosomatid was isolated from a plant, the lack of recognition of phytomonas-specific molecular markers based on spliced-leader and ribosomal genes as well as by monoclonal antibodies specific for phytomonas argues against assigning it to this genus. because the isolate displayed typical opisthomastigote forms in culture, it is assigned to the ...200911249194
crystallization and preliminary x-ray analysis of the glycerol-3-phosphate 1-acyltransferase from squash (cucurbita moschata).glycerol-3-phosphate 1-acyltransferase (e.c. 2.3.1.15; g3pat) catalyses the incorporation of an acyl group from either acyl-acyl carrier proteins (acylacps) or acylcoas into the sn-1 position of glycerol 3-phosphate to yield 1-acylglycerol 3-phosphate. crystals of squash g3pat have been obtained by the hanging-drop method of vapour diffusion using peg 4000 as the precipitant. these crystals are most likely to belong to space group p2(1)2(1)2(1), with approximate unit-cell parameters a = 61.1, b ...200111223529
a second gene for acyl-(acyl-carrier-protein): glycerol-3-phosphate acyltransferase in squash, cucurbita moschata cv. shirogikuza(*), codes for an oleate-selective isozyme: molecular cloning and protein purification studies.a new isogene for acyl-(acyl-carrier-protein):glycerol-3-phosphate acyltransferase (gpat; ec 2.3.1.15) in squash has been cloned and the gene product was identified as oleate-selective gpat. using pcr primers that could hybridise with exons for a previously cloned squash gpat, we obtained two pcr products of different size: one coded for a previously cloned squash gpat corresponding to non-selective isoforms at2 and at3, and the other for a new isozyme, probably the oleate-selective isoform at1. ...200011134424
crystallization and preliminary crystallographic study of cucurmosin, a ribosome-inactivating protein from the sarcocarp of cucurbita moschata.cucurmosin, a ribosome-inactivating protein purified from pumpkin, the sarcocarp of cucurbita moschata, has been crystallized using polyethylene glycol as a precipitant. the crystals belong to space group p2(1)2(1)2(1) and have unit-cell parameters a = 41.91, b = 59. 48, c = 98.78 a. there is one molecule in the asymmetric unit. the diffraction data to 3.0 a resolution were collected on a mar research image-plate detector.200010771444
the sterols of cucurbita moschata ("calabacita") seed oil.from the sterol fraction of seed oil from commercial cucurbita moschata dutch ("calabacita") delta 5 and delta 7 sterols having saturated and unsaturated side chain were isolated by chromatographic procedures and characterized by spectroscopic (1h and 13c-nuclear magnetic resonance, mass spectrometry) methods. the main components were identified as 24s-ethyl 5 alpha-cholesta-7,22e-dien-3 beta-ol (alpha-spinasterol); 24s-ethyl 5 alpha-cholesta-7,22e,25-trien-3 beta-ol (25-dehydrochondrillasterol) ...19968934454
butternut squash (cucurbita moschata) dermatitis. 19948187500
carotenoid composition and vitamin a value of a squash and a pumpkin from northeastern brazil.the carotenoid composition of a squash and a pumpkin from northeastern brazil was determined. nineteen carotenoids were detected in cucurbita moschata variety "baianinha"; beta-carotene was the principal carotenoid, contributing about 74% of an average total carotenoid content of 317.8 micrograms/g. in c. maxima variety "jerimum caboclo", 11 carotenoids were found with lutein, and beta-carotene as the major pigments accounting for about 60% and 27%, respectively, of an average total carotenoid c ...19902133187
the regulation of gelation of phloem exudate from cucurbita fruit by dilution, glutathione, and glutathione reductase.the average glutathione equivalent concentration in phloem exudate collected from squash fruit (cucurbita moschata [duchesne] poir. var butternut) and pumpkin fruit (cucurbita pepo [l.] var jack-o-lattern) was 1.02 and 0.60 millimolar, respectively. glutathione reductase (ec 1.6.4.2) activity in phloem exudate from squash and pumpkin fruit averaged 0.48 and 1.74 micromole nadph oxidized per minute per milliliter, respectively. protein concentrations in fruit phloem exudates averaged 67 milligram ...198816666036
chemical feeding deterrent mobilized in response to insect herbivory and counteradaptation by epilachna tredecimnotata.experimentally damaged leaves of cucurbita moschata mobilize substances to the damaged region within 40 minutes. these substances stimulate feeding by acalymma vittata (coleoptera: chrysomelidae) and inhibit feeding by epilacha tredecimnotata (coleoptera: coccinelidae). under natural conditions, epilachna cuts a circular trench in the leaf before feeding on the encircled leaf material, thus preventing mobilization of the deterrent substances to the feeding area.198017747815
d-amino-acid-stimulated ethylene production in seed tissues.ethylene production by axial and cotyledonary tissues excised from xanthium pennsylvanicum wallr. seeds was markedly (up to 5-fold) stimulated by the d-isomers of phenylalanine, valine, leucine, threonine, methionine and eithionine while the l-isomers caused no such effect. responsiveness of these seed tissues to d-methionine appeared soon after the beginning of imbibition, reached a maximum after 6-12 and 12-24 h for the axial and cotyledonary tissues, respectively, and then decreased sharply. ...198024306194
glutamate dehydrogenase from pumpkin cotyledons: characterization and isoenzymes.glutamate dehydrogenase from pumpkin (cucurbita moschata pior. cultivar dickinson field) cotyledons was found in both soluble and particulate fractions with the bulk of the activity in the soluble fraction. both enzymes used nad(h) and nadp(h) but nad(h) was favored. the enzymes were classified as glutamate-nad oxidoreductase, deaminating (ec 1.4.1.3). both enzymes were heat stable, had a ph optimum for reductive amination of 8.0, and were inhibited by high concentrations of nh(4) (+) or alpha-k ...197216657999
metabolism of arginine by aging and 7 day old pumpkin seedlings.the metabolism of arginine by etiolated pumpkin (cucurbita moschata) seedlings was studied over various time and age intervals by injecting arginine-u-(14)c into the cotyledons. at most, 25% of the (14)c was transported from the cotyledon to the axis tissue and the amount of this transport decreased with increasing age of the seedlings. the cotyledons of 25 day old plants contained 60% of the administered (14)c as unmetabolized arginine. little (14)c was in sugars and it appeared that arginine w ...196916657070
chemical studies on cucurbita moschata duch. ii. a synthesis of cucurbitine. 196113918398
chemical studies on cucurbita moschata duch. i. the isolation and structural studies of cucurbitine, a new amino acid. 196113891528
pumpkin seed (cucurbita moschata) in the treatment of acute schistosomiasis. 196013810019
amino acid profiling and antimicrobial activity of cucurbita moschata and lagenaria siceraria seed protein hydrolysates.cucurbita moschata and lagenaria siceraria seed proteins were extracted and hydrolysed with trypsin in order to recover antibacterial peptides. amino acid content and molecular weight distribution were estimated to justify their co-relationship with antimicrobial activity. antimicrobial activities of c. moschata and l. siceraria seed protein hydrolysates against three gram-negative bacteria and two gram-positive bacteria were evaluated. seed protein hydrolysates of both of these plants have sign ...201728783965
cytoprotective effects of pumpkin (cucurbita moschata) fruit extract against oxidative stress and carbonyl stress.background diabetes mellitus is a chronic endocrine disorder that is associated with significant mortality and morbidity due to microvascular and macrovascular complications. diabetes complications accompanied with oxidative stress and carbonyl stress in different organs of human body because of the increased generation of free radicals and impaired antioxidant defense systems. in the meantime, reactive oxygen species (ros) and reactive carbonyl species (rcs) have key mediatory roles in the deve ...028586926
salinity tolerance of grafted watermelon seedlings.in order to evaluate the salinity tolerance of grafted watermelon, two sets of experiments were conducted in a growing chamber where 'esmeralda' varieties were grafted onto interspecific squash (cucurbita maxima duch. × cucurbita moschata duch.) and lagenaria siceraria rootstocks. both non-grafted and self-garfted plants were used for control. for salt stress, 2.85 and 4.28 mm/l substrate doses of nacl were added with each irrigation in 2 day intervals for a duration of 23 days. interspecific-gr ...201729262705
evaluation of three pumpkin species: correlation with physicochemical, antioxidant properties and classification using spme-gc-ms and e-nose methods.to ascertain the most discriminant variables for three pumpkin species principal component analysis (pca) was performed. twenty-four parameters (ph, conductivity, sucrose, glucose, total soluble solids, l*, a*, b*, individual weight, edible rate, firmness, citric acid, fumaric acid, l-ascorbic acid, malic acid, ppo activity, pod activity, total flavonoids, vitamin e, total phenolics, dpph, frap, β-carotene, and aroma) were considered. the studied pumpkin species were cucurbita maxima, cucurbita ...201728974797
karyotype stability and unbiased fractionation in the paleo-allotetraploid cucurbita genomes.the cucurbita genus contains several economically important species in the cucurbitaceae family. here, we report high-quality genome sequences of c. maxima and c. moschata and provide evidence supporting an allotetraploidization event in cucurbita. we are able to partition the genome into two homoeologous subgenomes based on different genetic distances to melon, cucumber, and watermelon in the benincaseae tribe. we estimate that the two diploid progenitors successively diverged from benincaseae ...201728917590
transcriptome profiling of pumpkin (cucurbita moschata duch.) leaves infected with powdery mildew.cucurbit powdery mildew (pm) is one of the most severe fungal diseases, but the molecular mechanisms underlying pm resistance remain largely unknown, especially in pumpkin (cucurbita moschata duch.). the goal of this study was to identify gene expression differences in pm-treated plants (harvested at 24 h and 48 h after inoculation) and untreated (control) plants of inbred line "112-2" using rna sequencing (rna-seq). the inbred line "112-2" has been purified over 8 consecutive generations of sel ...201829320569
dehydrodiconiferyl alcohol promotes bmp-2-induced osteoblastogenesis through its agonistic effects on estrogen receptor.estrogen deficiency results in an imbalance between the levels of bone-resorping osteoclasts and bone-forming osteoblasts, eventually leading to overall bone loss. dehydrodiconiferyl alcohol (dhca), a lignan compound originally isolated from cucurbita moschata, has been shown to bind to estrogen receptor, and indeed exhibits various activities of estrogen, such as anti-inflammatory and anti-oxidative stress effects. in this study, we tested whether synthetic dhca could affect the bmp-2-induced o ...201829253565
ectopic expression of pumpkin nac transcription factor cmnac1 improves multiple abiotic stress tolerance in arabidopsis.drought, cold and salinity are the major environmental stresses that limit agricultural productivity. nac transcription factors regulate the stress response in plants. pumpkin (cucurbita moschata) is an important cucurbit vegetable crop and it has strong resistance to abiotic stress; however, the biological functions of stress-related nac genes in this crop are largely unknown. this study reports the function of cmnac1, a stress-responsive pumpkin nac domain protein. the cmnac1-gfp fusion protei ...201729234347
evaluation of the impact of food matrix change on the in vitro bioaccessibility of carotenoids in pumpkin (cucurbita moschata) slices during two drying processes.the food matrix is a limiting factor in determining the bioaccessibility of carotenoids. the impact of food matrix change on the bioaccessibility of carotenoids during drying processes is still unknown. the effect of intermittent microwave vacuum-assisted drying (imvd) and hot air drying (had) on the in vitro liberation and micellization of carotenoids in pumpkin slices was studied. this variable depended on the changes of the matrix driven by the drying process. different changes in the cell mo ...201729165479
can highly cited herbs in ancient traditional chinese medicine formulas and modern publications predict therapeutic targets for diabetes mellitus?the prevalence of diabetes among all age groups worldwide was estimated to be more than 382 million in 2013. traditional chinese medicine (tcm) has been practiced for thousands of years, and substantial valuable experience and prescriptions have been accumulated in the tcm system for the treatment of diabetes. in recent decades, a large amount of experimental and clinical data has been published on the use of herbal medicines related to these ancient tcm prescriptions.201829102765
a high-density linkage map and qtl mapping of fruit-related traits in pumpkin (cucurbita moschata duch.).pumpkin (cucurbita moschata) is an economically worldwide crop. few quantitative trait loci (qtls) were reported previously due to the lack of genomic and genetic resources. in this study, a high-density linkage map of c. moschata was structured by double-digest restriction site-associated dna sequencing, using 200 f2 individuals of cmo-1 × cmo-97. by filtering 74,899 snps, a total of 3,470 high quality snp markers were assigned to the map spanning a total genetic distance of 3087.03 cm on 20 li ...201728986571
evaluation of phytochemicals, antioxidant and burn wound healing activities of cucurbita moschata duchesne fruit peel.cucurbita moschata duchesne (pumpkin) is a well-known plant with several pharmacological effects. the aim of the present study was to assess burn wound healing activity of c. moschata peel extract (ce). also, standardized ce was assessed for antioxidant activity and antibacterial effects against major pathogens of burns.201728852445
a green analytical method for benzoyl peroxide determination by a sequential injection spectrophotometry using natural reagent extracts from pumpkin.a green and environmental friendly method with simple sequential injection spectrophotometry using natural reagent extract from pumpkin (cucurbita moschata decne) for determination of benzoyl peroxide (bpo) was developed. the maximum absorption of the yellow crude extract containing the β-carotene compound occurred at a wavelength of 450nm. the yellow extract solution was bleached by bpo to change the color to a colorless solution. the optimum conditions of the proposed method, such as extractio ...201728551134
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