| studies on dextranase. vi. some physicochemical properties and amino acid compositions of dextranases from brevibacterium fuscum var. dextranlyticum and peniclllium funiculosum iam 7013. | | 1975 | 1182875 |
| studies on dextranase. vii. the kinetic parameters of brevibacterium fuscum dextranase and molecular properties of the digestion products. | | 1975 | 1182887 |
| [formation of primary alcohols and palmitic acid in the microbiological oxidation of hexadecane]. | forty seven microbial strains oxidized hexadecane to form primary cetol and palmitic acid. the maximum quantitiy of the compounds was accumulated in the exponential phase of growing cultures (for 72 hours) and for 6 to 16 hours of incubation of resting cell suspensions. candida yeast were shown by the gas-liquid chromatography method to be the most active producers of cetol. | 1975 | 1187568 |
| [effect of the stereoisomers of glutamic acid on the vital activity of auxotrophic mutant producers of lysine]. | studies of l, d and dl-forms of glutamic acid as the sole nitrogen source or as a supplement to the major nitrogen source--ammonium sulphate have demonstrated that in the first case all forms of glutamic acid are assimilated by auxotrophic mutants--lysine producers. however, the lysine synthesis was very low, when l-glutamic acid was used as the only nitrogen source. glutamic acid at a concentration of 0.01 m applied as a supplement to the major source of nitrogen acted as a stimulator of lysine ... | 1975 | 1187569 |
| [microbiological transformation and degradation of pesticides]. | | 1975 | 1206144 |
| [study of various biochemical characteristics of bacteria from the genus brevibacterium breed, 1953]. | | 1975 | 1214647 |
| [activity of microorganisms decomposing cholesterol]. | the rates of cholesterol decomposition was compared among cell suspensions of different microorganisms. fifty seven cultures were studied: 2 strains of actinomycetes, 23 strains of proactinomycetes, 22 strains of mycobacteria, and 10 strains of bacteria. during four hours of incubation, 11 strains virtually did not decompose cholesterol at all, 10 strains decomposed it by up to 20 per cent, 21 strains by 20 to 70 per cent, and 15 strains by 70 to 100 per cent. the highest activity was displayed ... | 1975 | 1226133 |
| [generation time of pure cultures of heterotrophic microorganisms isolated from lake baĭkal]. | generation time was determined in pure cultures of heterotrophic microorganisms in the conditions similar to those of baikal in june--july of 1972. generation time was found to be 37+/-7, 16+/-2.5, 16+/-3.2, and 10+/-2.5 hours, respectively, when the cultures had been diluted with baikal water in the following rations: 1 : 0,1 : 5,1 : 10, and 1 : 20. no differences in the growth rate were found among 11 cultures of heterotrophic microorganisms isolated from baikal. conditions limiting the microb ... | 1975 | 1240574 |
| the ability of bacteria to synthesize a new cyclopyrophosphate correlates with their tolerance to redox-cycling drugs: on a crossroad of chemotherapy, environmental toxicology and immunobiochemical problems. | many redox-cyclers were recently shown to induce, in some bacterial species, large-scale biosynthesis of a new 2-methylbutan-1,2,3,4-tetraol-2,4-cyclopyrophosphate believed to be involved in anti-stress reactions. in the present study mycobacterium smegmatis, micrococcus luteus and brevibacterium ammoniagenes were shown to begin synthesis of the new cyclopyrophosphate when cultivated in a medium containing furacilin or furadonin (widely used nitrofuran antibacterial drugs) and to maintain close ... | 1992 | 1292477 |
| a new cyclopyrophosphate as a bacterial antistressor? | in a number of bacteria an unusual glycosyl pyrophosphate (31p nmr signal chemical shift at about -15 ppm) was detected when the cells were subjected to oxidative stress. this substance from brevibacterium ammoniagenes has now been identified as 2-methyl-butan-1,2,3,4,-tetraol-2,4-cyclopyrophosphate, which is accumulated in the cell under certain conditions in concentrations of of about 50 mm. it is now suggested that this compound is the long sought after bacterial antistressor. | 1992 | 1312021 |
| assay for n-acetylmuramyl-l-alanine amidase in serum by determination of muramic acid released from the peptidoglycan of brevibacterium divaricatum. | a method is reported for the determination of n-acetylmuramyl-l-alanine amidase in serum. muramic acid, released from the interpeptide bridges of brevibacterium divaricatum peptidoglycan, is measured by a modified colorimetric method. using this procedure, it was possible to determine n-acetylmuramyl-l-alanine amidase in aliquots of less than 10 microliters human serum with an incubation time of 10 min. amidase activity was found in all the sera tested (n = 11). the relevance of this simple and ... | 1992 | 1350922 |
| a flow injection analysis system involving immobilized nadh oxidase in column form for clinical analysis. | a highly sensitive fia system for chemiluminometric determination of reduced coenzyme, nadh, was developed, using immobilized nadh oxidase from brevibacterium ammoniagenes. the enzyme catalyzed the oxidation of nadh generating hydrogen peroxide which emitted chemiluminescence when mixed with luminol and potassium ferricyanide. the immobilized enzyme reactor was a mini-column, measuring 1 or 2 mm in inner diameter and 20 mm in length, and the sample volume was only 1 microliter per assay, with a ... | 1990 | 1366446 |
| electrotransformation of intact cells of brevibacterium flavum mj-233. | electroporation allowed transformation of intact cells of brevibacterium flavum mj-233. the two plasmids used for electroporation were pcry2 (6.3 kilobase) and pcry3 (8.2 kilobases). both plasmids contain the chloramphenicol-resistance gene and the autonomous replication origin in b. flavum mj-233. the efficiency of electrotransformation was optimal with cells harvested at the middle log phase of growth, and was improved by the addition of 1.0 u/ml of penicillin g to the culture medium. the opti ... | 1990 | 1366679 |
| construction of vector of brevibacterium lactofermentum and study of its stability in continuous culture. | a 5.7-kb vector plasmid pbk2 was constructed by ligating the kanamycin resistance gene from escherichia coli plasmid pacyc177 to an endogenous cryptic 4.4-kb plasmid of brevibacterium lactofermentum atcc 21086. the vector replicates efficiently and is stably maintained in the host and other coryneforms. however, the copy number varied from 50 to 10 per chromosome-equivalent under different culture conditions. continuous culture studies showed instability when low dilution rates were used. co-cul ... | 1990 | 1366813 |
| n-terminal amino acid sequence of brevibacterium sp. r312 wide-spectrum amidase. | a wide-spectrum amidase from brevibacterium sp. r312 was partially purified. the enzyme subunit was purified by reversed phase hplc and the n-terminal amino acid sequence was found to be identical to that of pseudomonas aeruginosa aliphatic amidase. | 1991 | 1368108 |
| electroporation-transformation system for coryneform bacteria by auxotrophic complementation. | we evaluated electroporation as an alternative system for genetic exchange for one of the coryneform bacteria, brevibacterium flavum mj233. the maximum number of transformants, 6 x 10(4) cells, was obtained when cells were cultured with penicillin g (1 u/ml) and harvested at the middle-log phase. electroporation was done using 12.5 kv/cm of pulse field strength, 1 x 10(10) cells, and 1 microgram of plasmid dna. other coryneform bacteria, brevibacterium lactofermentum atcc 13869, corynebacterium ... | 1990 | 1368509 |
| purification and properties of purine nucleoside phosphorylase from brevibacterium acetylicum atcc 954. | purine nucleoside phosphorylase of brevibacterium acetylicum atcc 954, which catalyzes the production of ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), a potent antiviral agent, from purine nucleoside and 1,2,4-triazole-3-carboxamide in a high yield, was purified 49-fold. this enzyme had a molecular weight of 31,000 and was a monomer. the isoelectric point of the enzyme was 4.7. the optimal temperature and ph of inosine phosphorolyzing reaction catalyzed by the enzyme was aroun ... | 1991 | 1368697 |
| hyperexpression and analysis of chob encoding cholesterol oxidase of brevibacterium sterolicum in escherichia coli and streptomyces lividans. | we examined the expression of chob, encoding cholesterol oxidase of brevibacterium sterolicum atcc 21387, in escherichia coli jm105 and streptomyces lividans tk23 using various deletion dna fragments within the 5'-flanking region. the enzyme activity could be detected intracellularly in e. coli only when the 5'-flanking region was reduced to less than 256-bp and chob was transcribed by the lac promoter. a large amount of the enzyme were produced as inactive inclusion bodies when chob protein was ... | 1992 | 1369073 |
| expression of streptomyces genes encoding extracellular enzymes in brevibacterium lactofermentum: secretion proceeds by removal of the same leader peptide as in streptomyces lividans. | the alpha-amylase gene (amy) from streptomyces griseus imru 3570 and the beta-galactosidase gene (lac) from s. lividans were subcloned into brevibacterium lactofermentum or b. lactofermentum/escherichia coli shuttle vectors. the amy gene was not expressed in b. lactofermentum from its own promoter but was efficiently expressed when the promoter of the kanamycin resistance gene (kan) was inserted upstream of the promoterless amylase gene. the lac gene from s. lividans was subcloned without its na ... | 1992 | 1369160 |
| improvement in microbial production of l-tyrosine by gene dosage effect of arol gene encoding shikimate kinase. | | 1990 | 1369260 |
| genetic breeding of l-tyrosine producer from brevibacterium lactofermentum. | a wild-type parent of brevibacterium lactofermentum was converted into an l-tyr producer by three steps of genetic breeding. first, acquirement of m-fluoro-d, l-phenylalanine resistance (1,000 microgram/ml) brought about mf1317 which produced 3.5 g/l of l-tyr and a byproduct of 2.8 g/l of l-phe. second, increase in the drug resistance (5,000 microgram/ml) gave mf358 that produced 6.4 g/l of l-tyr and a byproduct of 6.0 g/l of l-phe. third, an l-phe auxotrophic mutant (ft-1) derived from mf358 ac ... | 1990 | 1369436 |
| cloning and characterization of genes responsible for m-fluoro-d,l-phenylalanine resistance in brevibacterium lactofermentum. | two kinds of 3-deoxy-d-arabino-hepturosonate-7-phosphate (dahp) synthase genes were cloned from an l-phe-producing mutant of brevibacterium lactofermentum, aj11957, which was resistant to m-fluoro-d,l-phenylalanine (mfp) and p-fluoro-d,l-phenylalanine (pfp) and which had dahp synthase free from feedback inhibition. both genes were cloned using a vector plasmid, paj1844, and the resulting recombinant plasmids were named par1 and par2. they had different structures on the restriction maps. both pl ... | 1990 | 1369437 |
| the bleomycin resistance gene of transposon tn5 is an excellent marker for transformation of corynebacteria. | corynebacteria are highly sensitive to the glycopeptide antibiotic bleomycin. the bleomycin resistance gene of transposon tn5 is expressed very efficiently in brevibacterium lactofermentum. this gene constitutes an excellent marker for selection of transformants of corynebacteria. the bleomycin resistance gene is expressed from the same promoter as the neomycin resistance gene, which is already used as marker in many vectors of corynebacteria. the promoter of the neo-ble cluster is expressed in ... | 1992 | 1373065 |
| resonance raman studies of the protocatechuate 3,4-dioxygenase from brevibacterium fuscum. | resonance raman studies of the protocatechuate 3,4-dioxygenase (pcd) from brevibacterium fuscum have been carried out to take advantage of the high iron-site homogeneity of this enzyme. native uncomplexed pcd exhibits individual resonance-enhanced nu co and delta ch vibrations for the two tyrosinates coordinated to the active site iron center, which can be assigned to a particular residue by their excitation profiles. of the two nu co features observed at 1254 and 1266 cm-1, only the latter is u ... | 1992 | 1420163 |
| [changes in the intensity of dna synthesis in immunocompetent mouse tissues by peptidoglycans of bacterial origin]. | the data are presented on the effect of activation of by peptidoglycans from cell walls of staphylococcus aureus and brevibacterium flavum on the dna synthesis intensity in the mice spleen, thymus and bone marrow in vivo. maximum of the peptidoglycan influence on the dna synthesis intensity is observed 12-36 hours after injection. as a rule, higher doses of the studied peptidoglycans (1 mg per one animal) activate the dna synthesis more efficiently. peptidoglycan from br. flavum stimulates the d ... | 1992 | 1440964 |
| [the characteristics of the polyclonal action and immunomodulating activity of bacterial peptidoglycans in oppositely reacting mouse strains]. | the data on the specific features of the polyclonal action of gram-negative bacteria on primary immune response to sheep red blood cells in oppositely reacting mouse strains are presented. staphylococcus aureus and bacterium flavum peptidoglycans have been found to increase the amount of antibody producing cells to thymus-dependent antigen only in the spleen of low-responsive mice. at the same time differences in the amino acid composition of these heteropolymers do not affect their stimulating ... | 1992 | 1441816 |
| antibacterial activity of totarol and its potentiation. | antimicrobial activity of six diterpenoids isolated from the bark of podocarpus nagi (podocarpaceae) has been tested against twelve selected microorganisms. totarol [1], the most abundant compound among the six, exhibited potent bactericidal activity only against gram-positive bacteria, among which propionibacterium acnes was the most sensitive bacterium. totarol also showed strong activity against four other gram-positive bacteria tested: streptococcus mutans, bacillus subtilis, brevibacterium ... | 1992 | 1453180 |
| isolation of promoter sequences from brevibacterium sp. r312. | promoter sequences recognized by escherichia coli rna polymerase were isolated from brevibacterium sp. r312, a coryneform strain producing nitrile hydratase and amidase. ten escherichia coli clones containing promoter sequences were selected for their ability to grow with chloramphenicol concentrations of up to 1500 micrograms/ml. the strength of these promoter sequences was determined. we carried out a preliminary study of the strongest promoter having a chloramphenicol acetyl-transferase/beta- ... | 1992 | 1471439 |
| culture media for non-sporulating gram-positive food spoilage bacteria. | the spoilage association especially of protein-rich foods can be dominated by gram-positive bacteria, notably lactic acid bacteria (lab) which affect vacuum packaged refrigerated processed meats and some dairy products. new food ecosystems are being created by novel packaging and processing technologies, resulting in spoilage associations differing from those previously reported. in addition, improvement in identification methods, allow the detection and isolation of 'novel' bacterial groups, e. ... | 1992 | 1486021 |
| characterization of bacteriophage bfk20 from brevibacterium flavum. | bacteriophage bfk20 was isolated from a brevibacterium flavum strain that had become contaminated during industrial fermentation. bfk20 has a polyhedral head 50 nm wide and a non-contractile tail 200 nm long and 10 nm in diameter. the genome of this bacteriophage consists of a linear double stranded dna molecule of 44-45 kb with cohesive ends. the capsid of phage bfk20 contains nine polypeptides with molecular masses from 22.0-108.0 kda. bfk20 dna was used as a donor for fragments carrying promo ... | 1992 | 1512569 |
| characterization of aerobic non-lipophilic coryneforms from human feet. | aerobic coryneform bacteria from human feet have been studied by analysis of the cell-wall sugars, lipids and diamino acids and by phenotypic tests. although many isolates studied fall into the established genus brevibacterium at least two previously unreported taxa of coryneform have been identified bringing the number of aerobic, non-lipophilic taxa known on skin to four. simple tests can be used to distinguish between these taxa. studies on the human skin flora continue to reveal the diversit ... | 1992 | 1516232 |
| [isolation and properties of intracellular peptidase from brevibacterium]. | the intracellular peptidase of brevibacterium e531, a lysine-producing bacterial species, was purified 6500-fold by chromatography on deae-cellulose and the affinity adsorbent h-thr(but)-phe-pro-hexamethylene-diamine-sepharose 4b and by gel filtration on sephadex g-200. the enzyme displayed the maximum activity towards proline p-nitroanilide at ph 7.7-7.9 and readily split glycine, alanine and proline from di-, tri- and tetrapeptides but did not practically hydrolyze oligopeptides of a greater c ... | 1992 | 1525240 |
| the n-terminal amino acid sequences of brevibacterium sp. r312 nitrile hydratase. | nitrile hydratase from brevibacterium sp. r312 was purified to homogeneity. the isoelectric point was 5.75. the two kinds of subunits were separated by reverse phase hplc and their n-terminal amino acid sequences were found to be identical to those of rhodococcus sp. n-774 nitrile hydratase. | 1992 | 1527703 |
| enzyme-catalyzed oxidation of cholesterol in pure monolayers at the air/water interface. | mean molecular area vs. lateral surface pressure isotherms were determined for monolayers containing cholesterol, 4-cholesten-3-one (cholestenone), or binary mixtures of the two. at all lateral surface pressures examined, cholestenone had a larger mean molecular area requirement than cholesterol. results with the binary mixtures of cholesterol and cholestenone suggested that the sterols did not mix ideally (non additive mean molecular area) with each other in the monolayer; the observed mean mol ... | 1992 | 1536872 |
| antimicrobial activity of sphingosines. | the antimicrobial activity of stratum corneum lipids was examined by screening in vitro various representative phospholipids and sphingolipids. of mixed galacto-cerebrosides; phosphatidic acid; phosphatidic acid-monomethylester-dioleoyl; phosphatidylethanolamine; phosphatidylethanolamine-beta-oleoyl-gamma-palmitoyl; phosphatidylcholine; d-sphingosine; d,l-sphinganine; 4-d-hydroxysphinganine; oleoyl-sphingosine; n,n-dimethylsphingosine; and stearylamine, only the sphingosines and, to a lesser ext ... | 1992 | 1545135 |
| molecular structure of the multifunctional fatty acid synthetase gene of brevibacterium ammoniagenes: its sequence of catalytic domains is formally consistent with a head-to-tail fusion of the two yeast genes fas1 and fas2. | the brevibacterium ammoniagenes fatty acid synthetase (fas) gene was isolated from a series of overlapping clones by both immunological and plaque hybridization screening of two independent gene libraries. from the isolated dna a contiguous segment of 10,549 bp was sequenced in both directions. the sequenced dna contained a very long (9312 nucleotides) open reading frame coding for a protein of 3104 amino acids and with a molecular mass of 327,466 daltons. based on characteristic sequence motifs ... | 1992 | 1552898 |
| synthesis of a new organic pyrophosphate in large quantities is induced in some bacteria by oxidative stress. | brevibacterium ammoniagenes and micrococcus luteus were shown to synthesize up to 50 mm of a novel substance, 2-methylbutan-1,2,3,4-tetraol 2,4-cyclopyrophosphate, in response to oxidative stress created by benzyl viologen and other redox mediators under aerobic conditions. the substance, which represents greater than 50% of the extractable phosphorus, is suggested to play a role as a bacterial antistressor and is thought to be a product of condensation of two molecules of phosphoenolpyruvate wh ... | 1992 | 1605835 |
| [study of phage bbl1 of brevibacterium flavum and construction of a plasmid containing the cos-sequence of bbl1]. | | 1992 | 1620152 |
| cloning and primary structure of the wide-spectrum amidase from brevibacterium sp. r312: high homology to the amie product from pseudomonas aeruginosa. | a brevibacterium sp. r312 dna fragment encoding the wide-spectrum amidase (ec 3.5.1.4) has been cloned and sequenced, using limited amino acid (aa) sequence information obtained from the purified enzyme. the deduced aa sequence showed more than 80% strict identity with the pseudomonas aeruginosa aliphatic amidase, the product of the amie gene, suggesting a horizontal transfer of the gene during evolution between gram+ and gram- bacteria. | 1992 | 1628849 |
| changes in fatty acid branching and unsaturation of streptomyces griseus and brevibacterium fermentans as a response to growth temperature. | streptomyces griseus showed three different modes of changing fatty acids in response to temperature change. in brevibacterium fermentans, two such responses were found. the responses involved changes in fatty acid branching, unsaturation, or chain length, depending on growth temperature range. changes in unsaturation of branched-chain acids were characteristic at low growth temperatures. | 1992 | 1637171 |
| 'integron'-bearing vectors: a method suitable for stable chromosomal integration in highly restrictive corynebacteria. | a pbr322-derived plasmid (pcgl107) that carries the corynebacterium melassecola atcc17965 analogue of escherichia coli gdha gene (encoding glutamate dehydrogenase), was introduced into the related strain, brevibacterium lactofermentum cgl2002, by electroporation and integrated into its chromosome by homologous recombination. however, pcgl107 cannot integrate into c. melassecola, since the host restriction prevents successful electroporation by e. coli-modified dna. nevertheless, b. lactofermentu ... | 1991 | 1660430 |
| stabilization of a miniderivative of the broad-host-range incw plasmid psa by insertion of plasmid r1 parb region. | the plasmid vector pem100 (13.5 kb) constructed from pgv1106, a miniderivative of the broad-host-range incw psa plasmid, and the pam330 plasmid of brevibacterium lactofermentum is not stably maintained in escherichia coli host cells under nonselective growth conditions. by insertion of a 0.9 kb dna fragment containing the parb locus (responsible for the maintenance of plasmid r1 in e. coli cells) to plasmid pem100, plasmid pem110 was prepared which is maintained in a population of e. coli cells ... | 1991 | 1668749 |
| discrimination of corynebacterium glutamicum, brevibacterium flavum and brevibacterium lactofermentum by restriction pattern analysis of dna adjacent to the hom gene. | different strains of corynebacterium glutamicum, brevibacterium flavum, and brevibacterium lactofermentum were analysed for restriction fragment length polymorphism using the homoserine dehydrogenase gene (hom) as a probe. the hybridization patterns obtained pvuii- or asp700-restriction of chromosomal dna were specific and distinguishable for each of the three species and identical for the different strains of each species. thus, the method employed allows rapid distinction of corynebacterium gl ... | 1991 | 1682208 |
| a pyruvate-stimulated adenylate cyclase has a sequence related to the fes/fps oncogenes and to eukaryotic cyclases. | the pyruvate-stimulated adenylate cyclase from brevibacterium liquefaciens produces up to 450 microm cyclic amp in the culture medium when the bacterium is grown on glucose and alanine. in this paper we report the cloning, expression and sequencing of the gene for this enzyme. residues were identified, within the c-terminal domain, which are conserved in adenylate and guanylate cyclase sequences from eukaryotes and in the adenylate cyclase of the prokaryote rhizobium meliloti. we have also ident ... | 1991 | 1683468 |
| transfer of brevibacterium divaricatum dsm 20297t, "brevibacterium flavum" dsm 20411, "brevibacterium lactofermentum" dsm 20412 and dsm 1412, and corynebacterium glutamicum and their distinction by rrna gene restriction patterns. | the results of dna-dna hybridization and chemotaxonomic studies indicated that the glutamic acid producers brevibacterium divaricatum dsm 20297t (t=type strain), "brevibacterium flavum" dsm 20411, "brevibacterium lactofermentum" dsm 1412 and dsm 20412, corynebacterium lilium dsm 20137t, and corynebacterium glutamicum dsm 20300t and dsm 20163 are members of the same species. it is proposed that all of these strains should be classified in the species corynebacterium glutamicum. another glutamic a ... | 1991 | 1713055 |
| improved vectors for transcriptional signal screening in corynebacteria. | new promoter-probe and terminator-probe shuttle vectors for escherichia coli and corynebacteria were constructed. these vectors, working with the uida gene of e. coli as reporter gene, are very useful for the cloning and subsequent analysis of transcriptional regulatory signals. the beta-glucuronidase encoding activity of uida can be easily detected on agar plates containing chromogenic substrates such as 5-bromo-4-chloro-3-indolyl-beta-d-glucuronide (x-gluc). in the terminator-probe vector put2 ... | 1991 | 1722768 |
| cholesterol heterogeneity in the plasma membrane of epithelial cells. | the distribution of cholesterol in the plasma membrane of epithelial cells has been determined using renal brush border vesicles as a model. in brush borders treated with brevibacterium sp. or nocardia erythropolis cholesterol oxidases, a significant fraction of the free cholesterol was oxidized rapidly, without glutaraldehyde fixation, and the remaining cholesterol was oxidized at a slower rate. the size of the readily accessible cholesterol pool, however, depended on the enzyme used, varying f ... | 1992 | 1731911 |
| structural organization of the corynebacterium glutamicum plasmid pcg100. | pcg100, a 3 kb cryptic plasmid of corynebacterium glutamicum atcc 13058, probably identical with psr1 from c. glutamicum atcc 19223, was characterized. the minimum region for autonomous replication was shown to be contained on a 1.9 kb bglii-ncoi fragment; a 380 bp hindiii-sphi fragment can replicate in the presence of the parental plasmid, which presumably provides a trans-acting replication factor. derivatives of pcg100 are able to replicate in several corynebacterium, brevibacterium and arthr ... | 1991 | 1748866 |
| central venous line infection caused by brevibacterium epidermidis. | | 1991 | 1753126 |
| [study of brevibacterium flavum phage phibsh6]. | properties of a virulent brevibacterium flavum bacteriophage phi bsh6 were studied. the phage was placed in morphological group b1 according to ackerman classification, head diameter being 74-3 nm, tail length being 337 +/- 15 nm. the phage was shown to have double stranded dna as a genetic material. the chromosome is linear having cohesive ends. chromosome length was estimated to be about 71 kbp by restriction analysis and electron microscopy. a unique ecori-ecori fragment of bacteriophage dna ... | 1991 | 1758473 |
| analysis of the promoter region of saf, a streptomyces griseus gene that increases production of extracellular enzymes. | the product of the saf gene of streptomyces griseus atcc10137 mediated an increase in the production of several extracellular enzymes and retarded the formation of pigments and spores in streptomyces [daza et al., mol. gen. genet. 222 (1990) 384-392]. a promoter upstream from saf was identified by subcloning a dna fragment in the promoter probe pij486. using the escherichia coli-brevibacterium lactofermentum promoter-probe shuttle vector, pulmj51, we determined that the saf promoter region is al ... | 1991 | 1761232 |
| the derepression of enzymes of de novo pyrimidine biosynthesis pathway in brevibacterium ammoniagenes producing uridine-5-monophosphate and uracil. | a mutant of brevibacterium ammoniagenes producing large quantities of ump and uracil is described. the mutations render bacteria braditrophic for arginine, sensitive to adenine, resistant to rifampicin and pyrimidine analogues 5-fluorouracil, 5-fluorouridine, azauracil and thiouracil. the activities of enzymes involved in the ump biosynthesis, i.e. orotate phosphoribosyltransferase, orotate-5-monophosphate decarboxylase, dihydroorotate oxidase, are 4-, 3.5- and 4.5-fold higher in the mutant than ... | 1991 | 1769522 |
| a competitive radioimmunoassay for peptidoglycan monomer. | the preparation and characterization of the essential components to be used in the radioimmunoassay of peptidoglycan monomer (pgm) is described. in order to raise the anti-peptidoglycan monomer antibodies 14c-labelled peptidoglycan monomer-bovine serum albumin conjugate was prepared by the coupling of 14c-peptidoglycan monomer to bovine serum albumin in the presence of glutaraldehyde in 0.1 m nahco3 at ph 8.3. the prepared conjugate elicited anti-pgm response in rabbits. a synthetic analog of pe ... | 1991 | 1807868 |
| [potential vectors for molecular cloning in brevibacterium flavum]. | construction of the shuttle cloning vectors for escherichia coli-brevibacterium flavum system is described. expression of the sp/sm resistance determinant derived from the corynebacterium plasmid pcg4 was registered in escherichia coli cells. the genetic determinant for sp/sm resistance was shown to be located in a 2.2 kb psti-sphi fragment by the deletion analysis mapping in escherichia coli cells. using escherichia coli as a host we cloned the unique 0.8 kb ecori-ecori fragment of brevibacteri ... | 1991 | 1808511 |
| application of high-performance liquid chromatography to the study of the biological transformation of adiponitrile by nitrile hydratase and amidase. | a procedure for the assay of nitrile hydratase and amidase activities by high-performance liquid chromatography is described. the method can be used to assay the intermediate compounds resulting from the hydrolysis of adiponitrile to adipic acid, and to determine the kinetics of the hydrolysis of these compounds using whole cells and enzyme extracts. the precision of the method makes it suitable for the determination of the enzymic parameters. | 1991 | 1816746 |
| construction and characterization of promoter-probe vectors for corynebacteria using the kanamycin-resistance reporter gene. | several multicopy promoter-probe plasmid vectors have been constructed that replicate in brevibacterium lactofermentum and related coryneform amino acid-producing bacteria. transcriptional activity is detected by the expression of a promoter-less aminoglycoside phosphotransferase gene (kan) derived from transposon tn5; expression of this gene confers kanamycin resistance in b. lactofermentum. an efficient transcriptional terminator from the b. lactofermentum trp operon has been inserted upstream ... | 1991 | 1849494 |
| [contacts between the cells in bacterial colonies]. | the interaction of cells in microbial colonies has been studied by electron-microscopic techniques. two types of contacts between cells have been found to exist in the colonies of gram-negative bacteria of the genera escherichia, shigella and salmonella: close cell adhesion due to the fusion of cell-wall outer membranes and the formation of intersections consisting of membranous tubules. at the sites of close adhesion the fusion of cytoplasmic and outer membranes have been found to occur in baye ... | 1991 | 1867039 |
| sequence of gene chob encoding cholesterol oxidase of brevibacterium sterolicum: comparison with choa of streptomyces sp. sa-coo. | the nucleotide (nt) sequence of the cholesterol oxidase (cho)-encoding gene (chob) cloned from brevibacterium sterolicum atcc21387 was determined. the sequence contained an open reading frame with a g + c content of 64.9 mol% that would encode a protein of 552 amino acids (aa). comparison of the nt sequence of chob and deduced aa sequence to those of the cho-encoding gene (choa) of streptomyces sp. strain sa-coo showed identities of 64% and 58%, respectively. n-terminal aa sequence analysis of t ... | 1991 | 1879703 |
| [temperature changes of extracellular media state on a bacterial suspension]. | within temperature intervals 30-40 degrees c for bacterial suspension of e. coli and 24-34 degrees c for b. flavum the extracellular medium exists in a specific state. water in the extracellular medium is stabilized by increased hydrophobicity of extracellular protein molecules surface due to proteins conformational change. the total amount of uv-absorbing metabolites is decreased as a result of activation of microorganisms transport systems. the temperature intervals of these processes are diff ... | 1991 | 1892906 |
| cellular fatty acid composition as an adjunct to the identification of asporogenous, aerobic gram-positive rods. | cellular fatty acid (cfa) compositions of 561 asporogenous, aerobic gram-positive rods were analyzed by gas-liquid chromatography as an adjunct to their identification when grown on blood agar at 35 degrees c. the organisms could be divided into two groups. in the first group (branched-chain type), which included coryneform cdc groups a-3, a-4, and a-5; some strains of b-1 and b-3; "corynebacterium aquaticum"; brevibacterium liquefaciens; rothia dentocariosa; and listeria spp., the rods had siza ... | 1991 | 1899679 |
| antagonistic effect of coryneform bacteria from red smear cheese against listeria species. | a total of 187 coryneform bacteria were isolated from red smear and screened for inhibitory effects against 16 strains of listeria species. culture filtrates from brevibacterium linens (16 strains), arthrobacter nicotianae (4 strains) and arthrobacter nucleogenes (3 strains) showed clear zones of inhibition. the antagonistic effect was seen against 26 to 87% of 91 listeria strains tested. a. nicotianae and a. nucleogenes were more effective against listeria innocua and listeria ivanovii than aga ... | 1991 | 1909545 |
| cloning vectors and antibiotic-resistance markers for brevibacterium sp. r312. | replication of several cryptic plasmids from coryneform strains was investigated in brevibacterium sp. r312. only the corynebacterium glutamicum psr1 replicon was found to be suitable for establishing a host-vector system. two psr1 derivatives, prpcg200 and phycg1, were used as cloning vectors. they carry a neomycin-resistance-encoding and a tetracycline-resistance-encoding gene, respectively. | 1991 | 1937001 |
| purification, cloning, and primary structure of a new enantiomer-selective amidase from a rhodococcus strain: structural evidence for a conserved genetic coupling with nitrile hydratase. | a new enantiomer-selective amidase active on several 2-aryl propionamides was identified and purified from a newly isolated rhodococcus strain. the characterized amidase is an apparent homodimer, each molecule of which has an mr of 48,554; it has a specific activity of 16.5 mumol of s(+)-2-phenylpropionic acid formed per min per mg of enzyme from the racemic amide under our conditions. an oligonucleotide probe was deduced from limited peptide information and was used to clone the corresponding g ... | 1991 | 1938876 |
| 3-(2-hydroxyphenyl)catechol as substrate for proximal meta ring cleavage in dibenzofuran degradation by brevibacterium sp. strain dpo 1361. | brevibacterium sp. strain dpo 1361 oxygenates dibenzofuran in the unusual angular position. the 3-(2-hydroxyphenyl)catechol thus generated is subject to meta ring cleavage in the proximal position, yielding 2-hydroxy-6-(2-hydroxyphenyl)-6-oxo-2,4-hexadienoic acid, which is hydrolyzed to 2-oxo-4-pentenoate and salicylate by 2-hydroxy-6-oxo-6-phenyl-2,4-hexadienoic acid hydrolase. the proximal mode of ring cleavage is definitely established by isolation and unequivocal structural characterization ... | 1991 | 2001996 |
| identification of plasmid partition function in coryneform bacteria. | we have identified and characterized a partition function that is required for stable maintenance of plasmids in the coryneform bacteria brevibacterium flavum mj233 and corynebacterium glutamicum atcc 31831. this function is localized to a hindiii-nspv fragment (673 bp) adjacent to the replication region of the plasmid, named pby503, from brevibacterium stationis ifo 12144. the function was independent of copy number control and was not associated directly with plasmid replication functions. thi ... | 1991 | 2039232 |
| crystal structure of cholesterol oxidase from brevibacterium sterolicum refined at 1.8 a resolution. | cholesterol oxidase (3 beta-hydroxysteroid oxidase, ec 1.1.3.6) is an fad-dependent enzyme that carries out the oxidation and isomerization of steroids with a trans a : b ring junction. the crystal structure of the enzyme from brevibacterium sterolicum has been determined using the method of isomorphous replacement and refined to 1.8 a resolution. the refined model includes 492 amino acid residues, the fad prosthetic group and 453 solvent molecules. the crystallographic r-factor is 15.3% for all ... | 1991 | 2051487 |
| oxidation of cholesterol in low density and high density lipoproteins by cholesterol oxidase. | the cholesterol oxidase-catalyzed oxidation of cholesterol in native low density (ldl) and high density lipoproteins (hdl3) as well as in monolayers prepared from surface lipids of these particles, has been examined. the objective of the study was to compare the oxidizability of cholesterol, and to examine the effects of lipid packing on oxidation rates. when [3h]cholesterol-labeled lipoproteins were exposed to cholesterol oxidase (streptomyces sp.), it was observed that ldl [3h]cholesterol was ... | 1990 | 2090717 |
| ultrastructural features of microbial colony organization. | the ultrastructure of microbial colonies was studied. inside the colonies three types of intercellular contacts were demonstrated. in the colonies of gram-negative bacteria, the cells were found to be connected by tight adhesions of outer membranes of the cell walls and membrane bridges. in the colonies of gram-positive bacteria, the intercellular contacts were formed by fusion of peptidoglucan layers of the cell walls. bacterial cells were differentiated by the presence of a capsule-like envelo ... | 1990 | 2097346 |
| interspecies electro-transformation in corynebacteria. | plasmid dna was efficiently electro-transformed into intact cells of nine corynebacteria strains belonging to brevibacterium lactofermentum, brevibacterium flavum, corynebacterium glutamicum and corynebacterium melassecola. relationships were explored between transformation efficiency and parameters such as electric field strength and pulse length, dna concentration, physiological state and concentration of the cells. in optimal conditions, more than 10(7) transformants per microgram of dna coul ... | 1990 | 2108897 |
| [studies on ultraweak luminescence of bacteria]. | ultraweak luminescence of escherichia coli, bacillus subtilis and brevibacterium ammoniagenes was measured with high sensitive single photon counting equipment (made in china). the results obtained from ultraweak luminescence of as above three bacterial strains were as follows: spectral distribution curves, photon emission kinetic curves or emission intensity and its qualitative relationship between intensity and bacterial counts. | 1990 | 2111603 |
| strategies for the chemoenzymatic preparation of optically active 1-alkyn-3-ols. | a series of (r)- and (s)-1-alkyn-3-ols, chiral building units for the synthesis of leukotrienes and pheromones, were prepared via enantioselective hydrolysis of their racemic esters. while the majority of biocatalysts employed (lipases, fermenting or freeze-dried microorganisms) failed in discriminating between enantiomers, lyophilized cells of baker's yeast (saccharomyces cerevisiae hansen) gave (s)-1-alkyn-3-ols and their corresponding (r)-esters with greater than 90% e.e. | 1990 | 2113835 |
| cellular fatty acid composition of oerskovia species, cdc coryneform groups a-3, a-4, a-5, corynebacterium aquaticum, listeria denitrificans and brevibacterium acetylicum. | twenty four strains representing eight species of gram positive yellow-pigmented rods (oerskovia turbata, oerskovia xanthineolytica, cdc coryneform groups a-3, a-4, a-5, listeria denitrificans, corynebacterium aquaticum and brevibacterium acetylicum) were divided into two major groups based on the relative amounts of 12 methyltetradecanoate (15:0a) obtained by capillary gas liquid chromatography. o. turbata, o. xanthineolytica, cdc groups a-3 and a-4, l. denitrificans and c. aquaticum were place ... | 1990 | 2124793 |
| [lysine production by brevibacterium divaricatum ntu-2 and its recovery from the fermentation broth]. | an accumulation of l-lysine of about 42 g/l (as l-lysine-hcl) was obtained by cultivating brevibacterium divaricatum ntu-2 in a medium containing 10.0% glucose, 4.0% (nh4)2 so4, 0.1% kh2 po4, 0.04% mg so4.7h2 o, 30 ml/l soybean meal hydrolysate, 50 mg/l dl-methionine, 100 micrograms/l d-biotin, 100 micrograms/l thiamine-hcl and 5% caco3 at ph 7.0. the yield was about 48.8% based on consumed glucose. the l-lysine accumulated in the broth was recovered and purified by simply using a strong cation- ... | 1990 | 2128693 |
| plasmid shuttle vector with two insertionally inactivable markers for coryneform bacteria. | a new shuttle vector pcem500 replicating in escherichia coli and in brevibacterium flavum was constructed. it carries two antibiotic resistance determinants (kmr/gmr from plasmid psa of gram-negative bacteria and smr/spr from plasmid pcg4 of corynebacterium glutamicum) which are efficiently expressed in both hosts and can be inactivated by insertion of dna fragments into the unique restriction endonuclease sites located within them. this vector was found to be stably maintained in b. flavum and ... | 1990 | 2148164 |
| [development of the vector-host system in corynebacterium. cloning and expression of homoserine dehydrogenase and homoserine kinase genes in corynebacterium cells]. | novel cloning vectors for glutamic acid producing bacteria have been constructed. the cryptic plasmid pbo1 (4.4 kb) from brevibacterium sp. recombined with the plasmid pacyc184 (4.0 kb) from escherichia coli was used to produce composite plasmid named pka1. the plasmid could propagate and express the cm-r phenotype in e. coli and coryneform glutamic acid producing bacteria br. flavum, c. glutamicum, br. lactofermentum. the pka1 plasmid and its variants deleted within non-essential plasmid region ... | 1990 | 2165016 |
| [cloning and structural-functional analysis in escherichia coli of genes of glutamate-producing corynebacteria controlling biosynthesis of amino acids of aspartic acid series]. | a library of ecori dna fragments from brevibacterium flavum was constructed using plasmid vector. the genes complementing thra2 and thrb mutations in escherichia coli were identified in the library. the gene thra2 of b. flavum codes for mutant enzyme homoserine dehydrogenase insensitive to inhibition by threonine. the genes thra2 and thrb are localized wihtin the ecori fragment 4.1 kb long and are expressed under the control of their own promoters in e. coli cells. structural and functional anal ... | 1990 | 2191895 |
| molecular cloning, nucleotide sequence, and promoter structure of the acinetobacter calcoaceticus trpfb operon. | the trpfb operon from acinetobacter calcoaceticus encoding the phosphoribosyl anthranilate isomerase and the beta-subunit of tryptophan synthase has been cloned by complementation of a trpb mutation in a. calcoaceticus, identified by deletion analysis, and sequenced. it encodes potential polypeptides of 214 amino acids with a calculated molecular weight of 23,008 (trpf) and 403 amino acids with a molecular weight of 44,296 (trpb). the encoded trpb sequence shows striking homologies to those from ... | 1990 | 2211532 |
| construction of a promoter-probe shuttle vector for escherichia coli and brevibacteria. | we constructed a promoter-probe vector, pjup05, for brevibacteria and escherichia coli based on the promoterless neomycin-resistance (neor) gene from tn5. this gene confers resistance to the aminoglycosides, kanamycin and neomycin. the promoter of the neor gene was deleted and replaced by a suitable multiple cloning site. there are translation stop codons in all three reading frames upstream from the neor gene. the plasmid contains functional origins of dna replication for both brevibacteria and ... | 1990 | 2253886 |
| purification, cloning, and primary structure of an enantiomer-selective amidase from brevibacterium sp. strain r312: structural evidence for genetic coupling with nitrile hydratase. | an enantiomer-selective amidase active on several 2-aryl and 2-aryloxy propionamides was identified and purified from brevibacterium sp. strain r312. oligonucleotide probes were designed from limited peptide sequence information and were used to clone the corresponding gene, named amda. highly significant homologies were found at the amino acid level between the deduced sequence of the enantiomer-selective amidase and the sequences of known amidases such as indoleacetamide hydrolases from pseudo ... | 1990 | 2254253 |
| isolation and identification of the gene of cholesterol oxidase from brevibacterium sterolicum atcc 21387, a widely used enzyme in clinical analysis. | | 1990 | 2268339 |
| transfer of plasmid dna to brevibacterium lactofermentum by electrotransformation. | the escherichia coli-brevibacterium lactofermentum shuttle vector pbla was introduced into intact cells of b. lactofermentum by electrotransformation. several parameters of this procedure such as voltage and cell concentration were analysed. optimal conditions gave an efficiency of 10(6) transformants per microgram of dna. two recalcitrant strains could be electrotransformed when an ampicillin pretreatment step was used. electrotransformation experiments using dnaase or different structural form ... | 1990 | 2269876 |
| isolation and identification of the gene of cholesterol oxidase from brevibacterium sterolicum atcc 21387, a widely used enzyme in clinical analysis. | the gene coding cholesterol oxidase (chod) from brevibacterium sterolicum, which is widely used in clinical analysis, has been selected from puc19-based gene bank in e. coli mm294 by colony-hybridization using synthetic dna as probe. the gene was identified to encode the protein having the same amino acid sequence as that determined from amino-acid sequence analysis. the expression of the chod gene in e. coli was not observed, probably due to the transcription failure. attempts are being made to ... | 1990 | 2271066 |
| an exafs study of the interaction of substrate with the ferric active site of protocatechuate 3,4-dioxygenase. | x-ray crystallographic studies of the intradiol cleaving protocatechuate 3,4-dioxygenase from pseudomonas aeruginosa have shown that the enzyme has a trigonal bipyramidal ferric active site with two histidines, two tyrosines, and a solvent molecule as ligands [ohlendorf, d.h., lipscomb, j.d., & weber, p.c. (1988) nature 336, 403-405]. fe k-edge exafs studies of the spectroscopically similar protocatechuate 3,4-dioxygenase from brevibacterium fuscum are consistent with a pentacoordinate geometry ... | 1990 | 2271684 |
| synthesis of 17o- or 18o-enriched dihydroxy aromatic compounds. | | 1990 | 2280695 |
| protocatechuate 3,4-dioxygenase from brevibacterium fuscum. | | 1990 | 2280720 |
| isolation and characterization of a restriction and modification deficient mutant of brevibacterium lactofermentum. | in order to facilitate genetic engineering in amino-acid producing bacteria we have isolated two restriction-deficient brevibacterium lactofermentum strains. they have been selected for their ability to obtain a high yield of plaques from cl31 phage which was grown on corynebacterium lilium. these mutant strains do not restrict either phage dna by transfection or dna from the shuttle vector pbla extracted from escherichia coli by protoplast transformation. these mutants have also lost modificati ... | 1990 | 2283029 |
| microbial ecology of interdigital infections of toe web spaces. | the microbial flora of normal and macerated interdigital toe web spaces was qualitatively and quantitatively identified in 77 patients. dermatophyte fungi were recovered from 11% of normal patients compared with a 31% recovery from patients with macerated interspaces. macerated interspaces were characterized by a greater recovery of organisms with pathogenic potential, with staphylococcus aureus recovered from 36% of patients, micrococcus sedentarius in 37%, brevibacterium epidermidis in 54%, co ... | 1990 | 2319017 |
| 1h-n.m.r. studies of a natural immunoadjuvant peptidoglycan monomer: proposed structure in solution in methyl sulfoxide. | the conformation in solution in methyl sulfoxide of the immunoadjuvant peptidoglycan monomer (pgm), obtained by digestion with lysozyme of the linear peptidoglycan polymer isolated from brevibacterium divaricatum, was studied by 1h-n.m.r. spectroscopy. the temperature dependence of the chemical shift of the resonances of the amide protons suggested that the amino group of alanine-5 is involved in hydrogen bonding, most probably to the alpha-carbonyl of the isoglutamine which showed restricted ro ... | 1990 | 2346938 |
| characterization of phi ga1, an inducible phage particle from brevibacterium flavum. | eighteen related strains of arthrobacter, brevibacterium and corynebacterium were used as indicator strains in an attempt to isolate corynephages from a large number of soils and from waste-water samples. although no phages capable of producing plaques were isolated, one of the indicator strains used, brevibacterium flavum atcc 14067, was lysogenic for the inducible phage phi ga1. this phage was not observed to form plaques on any of the strains tested. phi ga1 is of b1-morphotype with a linear ... | 1990 | 2391491 |
| corynephage cog, a virulent bacteriophage of corynebacterium glutamicum, and its relationship to phi ga1, an inducible phage particle from brevibacterium flavum. | the host range of the virulent bacteriophage cog among several strains of amino acid-producing corynebacteria is limited to corynebacterium glutamicum lp-6. cog is a typical corynephage of the siphoviridae family (b1 morphotype) with an isometric head of 52 nm and a medium length, striated tail of 190 nm. it has a linear genome of 39.7 kb with cohesive ends and 53% g + c; a restriction map is presented, including regions of dna homology (by hybridization) with the inducible phage particle phi ga ... | 1990 | 2391495 |
| [the capacity of anaerobically grown bacteria to exchange the 2h+ of the cell for the k+ of the medium and to maintain a high k+ distribution between cell and medium]. | the n,n'-dicyclohexylcarbodiimide sensitive exchange of 2h+ of a cell for k+ of medium stable to ph, k+ activity and temperature changes has been discovered in anaerobically grown gram-negative escherichia coli, salmonella typhimurium. s. enteritidis, proteus mirabilis, p. vulgaris, anaerobic gram-positive bacteria streptococcus faecalis, lactobacillus salivarius, l. lactis in the presence of exogenic energy source. this exchange in gram-negative bacteria is operating only at increase of medium ... | 1986 | 2434147 |
| keratinolytic activity of cutaneous and oral bacteria. | a test was developed to measure the keratinolytic activity of cutaneous and oral bacteria. keratin, labeled with fluorescein isothiocyanate, was used in a phosphate buffer (ph 7.2) with 1 mm dithiothreitol. the degradation of keratin was estimated by measuring the fluorescence of the degradation products in the supernatant of the reaction mixtures in a luminescence spectrometer. several oral and cutaneous bacteria were investigated: bacteroides gingivalis, bacteroides intermedius, treponema dent ... | 1987 | 2434427 |
| nucleotide sequences of 5s ribosomal rnas of arthrobacter oxydans, arthrobacter polychromogenes, arthrobacter globiformis and 'brevibacterium helvolum'. | | 1988 | 2457874 |
| purification and properties of the beta-glucosidase from a nitrile hydratase-producing brevibacterium sp. strain r312. | besides its nitrile hydratase and wide spectrum amidase activities, the brevibacterium sp. r312 strain also possesses a constitutive beta-glucosidase. its optimum ph is 6. the enzyme was purified by fractionation precipitation with ammonium sulfate followed by chromatographic elutions on q-sepharose fast flow, sephadex g-200 and phenyl superose. the resulting purification was 1960 folds for a 6% yield. the molecular weight of this enzyme was estimated at 180,000. it contains two identical sub-un ... | 1989 | 2517305 |
| a fast spheroplast formation procedure in some 2,5-diketo-d-gluconate- and 2-keto-l-gulonate- producing bacteria. | calcium 2-keto-l-gulonate (ca-2-klg, a key intermediate in vitamin c synthesis) is produced from calcium 2,5-diketo d-gluconate (ca-2,5-dkg) by a variety of bacteria. a few bacterial species which efficiently convert glucose to ca-2,5-dkg have been isolated in our laboratory. our bacterial collection included species that possess the genes for production of ca-2-klg from ca-2,5-dkg; however, the yield of the former is poor. a procedure for the preparation of spheroplasts in ca-2,5-dkg- and ca-2- ... | 1989 | 2517394 |
| binding of isotopically labeled substrates, inhibitors, and cyanide by protocatechuate 3,4-dioxygenase. | binding of ligands to the active site fe3+ of protocatechuate 3,4-dioxygenase is investigated using epr-detected transferred hyperfine coupling from isotopically labeled substrates, inhibitors, and cyanide. broadening is observed in epr resonances from the anaerobic enzyme complex with homoprotocatechuate (3,4-dihydroxyphenylacetate), a slow substrate, enriched with 17o (i = 5/2) in either the 3-oh or the 4-oh group. this shows that this substrate binds directly to the fe3+ and strongly suggests ... | 1989 | 2542290 |
| derivatives of glutamic acid and trehalose which can be transformed into long-living free radicals by the loss of an electron are identified in some bacteria. | a derivative of glutamic acid (ammonigenin) and a trisaccharide named lysodektose which are converted into long-living free radicals by the loss of one electron were isolated from brevibacterium ammoniagenes and micrococcus lysodeikticus. structural formulae suggested for both substances based on esr-, nmr- and mass spectra, isotopic substitution experiments and other data are: lactone of n-hydroxy-n-(2-carbamoylethyl)-glutamyl-4-amino-2-hydroxybutyric amide and 6-o-[2-deoxy-2-(n-methyl)-hydroxy ... | 1989 | 2560374 |
| production of hydrogen sulphide in milkfat-coated microcapsules containing brevibacterium linens and cysteine. | milkfat-coated microcapsules containing brevibacterium linens and cysteine were used to produce hydrogen sulphide, one of cheddar cheese flavour compounds. hydrogen sulphide production was substantially reduced and delayed in the encapsulated system as compared with that of the unencapsulated system. hydrogen sulphide was not produced aerobically whereas substantial amounts of hydrogen sulphide were produced in a nitrogen purged system. the inhibitory effect of the initial aerobic condition disa ... | 1989 | 2585242 |
| dioxygenolytic cleavage of aryl ether bonds: 1,10-dihydro-1,10-dihydroxyfluoren-9-one, a novel arene dihydrodiol as evidence for angular dioxygenation of dibenzofuran. | two dibenzofuran degrading bacteria, brevibacterium strain dpo 1361 and strain dpo 220, were found to utilize fluorene as sole source of carbon and energy. cells which were grown on dibenzofuran, transformed fluorene into a number of products. for five of the seven metabolites isolated, the structure could be established unequivocally. accumulation of one metabolite, 1,10-dihydroxy-1,10-dihydrofluoren-9-one, indicated the presence of a novel type of dioxygenase, attacking polynuclear aromatic sy ... | 1989 | 2612886 |
| cloning and structure of the bepi modification methylase. | the gene coding for a cgcg specific dna methylase has been cloned in e. coli from brevibacterium epidermidis. the enzyme, named bepi methylase, is probably the cognate methylase of the fnudii isoschizomer bepi endonuclease isolated from this strain. the expression of bepi methylase in e. coli is dependent on the orientation of the cloned fragment suggesting that the gene is transcribed from a promoter on the plasmid vector. no bepi endonuclease could be detected in the clones producing bepi meth ... | 1989 | 2784204 |