| transport of neisseria meningitidis cultures: growth-supporting media and freezing as an alternative. | the abilities of transgrow (tg), thayer-martin (tm), and new york city (nyc) solid media to maintain the viability of 12 strains of neisseria meningitidis under various controlled conditions were assessed. the effects of charcoal impregnation of swabs, temperature, and an enriched co2 atmosphere were examined with holding for up to 21 days. recovery from samples held at 35 degrees c was, in almost all instances, greater than at 22 or 4 degrees c. a strong requirement for added co2 was demonstrat ... | 1979 | 108008 |
| immobilization of pyridoxal 5'-phosphate and pyridoxal 5'-phosphate-dependent enzymes on sepharose. | | 1979 | 108513 |
| esterification of cholesterol in human small intestine: the importance of acyl-coa:cholesterol acyltransferase. | human intestinal mucosa contains acyl-coa:cholesterol acyltransferase activity. the enzyme has been studied by using oleylcarnitine, coa and carnitine palmitoyltransferase as an oleyl-coa regenerating system. the enzyme was found in the particulate fraction of the cells, it had a ph optimum between 7.2 and 8.2, and was inhibited by taurocholate. the specific enzymic activity in biopsies from intestinal mucosa of normal men was found to be 3.6 +/- 1.37 nmol cholesteryl ester formed mg protein-1 h ... | 1979 | 110602 |
| membrane potential and reconstitution. | | 1979 | 110998 |
| infectious diarrhea. | | 1979 | 111270 |
| progressive-ratio performance in the rhesus monkey maintained by opiate infusions. | | 1979 | 111282 |
| sensitization of myelinated nociceptive afferents that innervate monkey hand. | | 1979 | 115969 |
| purification and characterization of a hemolysin produced by vibrio cholerae biotype el tor: another toxic substance produced by cholera vibrios. | a thermolabile direct hemolysin from an el tor cholera vibrio strain has been isolated and partially characterized as a simple protein of ca. 20,000 molecular weight. in addition to its hemolytic activity, the hemolysin is cytotoxic, cardiotoxic, and rapidly lethal. in these respects it resembles the thermostable direct hemolysin/cytotoxin/cardiotoxin/lethal toxin of vibrio parahaemolyticus and certain other bacterial hemolysins, although there are other significant differences. because identica ... | 1979 | 118926 |
| cellular immunity in protein malnutrition: differences in in vivo and in vitro responses. | | 1979 | 120012 |
| distribution of membrane-bound monoamine oxidase in bacteria. | the distribution of membrane-bound monoamine oxidase in 30 strains of various bacteria was studied. monoamine oxidase was determined by using an ammonia-selective electrode; analyses were sensitive and easy to perform. the enzyme was found in some strains of the family enterobacteriaceae, such as klebsiella, enterobacter, escherichia, salmonella, serratia, and proteus. among strains of other families of bacteria tested, only pseudomonas aeruginosa ifo 3901, micrococcus luteus ifo 12708, and brev ... | 1979 | 120132 |
| epidemiology of diarrhoeal diseases in singapore. | diarrhoeal diseases in the form of bacterial food poisoning are prevalent in singapore. common food poisoning organisms implicated were staphylococcus aureus, non-thypoid salmonellae and vibrio parahaemolyticus. el tor cholera, believed to be introduced through intra-regional trade and travel, occurred sporadically with a common source outbreak, probably food-borne, in september 1978. shigellosis and amoebiasis were mainly confined to areas where poor personal hygiene was prevalent. the incidenc ... | 1979 | 120734 |
| [oculomotor reflex evoked by vibrassae stimulation in the cat]. | the oculomotor effects of vibrissae stimulation have been studied in the "encéphale isolé" non-anaesthetized cat. intracellular recording of lateral rectus motoneurones showed an excitatory projection via the ipsilateral infraorbital nerve to these motoneurones. the afferent fibers are type a alpha, recuited with a low threshold (0.2-0.5 ma) and convey information about vibrissae displacement. | 1979 | 120789 |
| enzymic synthesis of steroid sulphates. xi. study of the oestrogen binding site of oestrogen sulphotransferase by affinity labelling with 4-mercuri-17beta-oestradiol. | oistrogen sulphotransferase (3"-phosphoadenylylsulphate: oestrone sulphotransferase, ec 2.8.2.4) contains asingle sulphydryl group thought to be at, or near, the oestrogen-binding site. 4-mercuri-17beta-oestradiol, the activity of the enzyme decreased with increasing concentration of the oestrogen derivative. however, some 40% of the activity remained when all the sulphydryl had reacted to form mercaptide. formation of mercaptide was only marginally decreased in the presence of the substrate 17b ... | 1975 | 123774 |
| inhibitory effect of normal mammalian sera on the rosette formation of human lymphocytes with sheep red blood cells. | | 1975 | 123840 |
| metabolic clearance rate, production rate and mammary uptake of progesterone in the goat. | the dynamics of progesterone uptake and metabolism in the mammary gland of the goat have been measured and related to the metabolic clearance rate and production rate of the hormone determined by tracer kinetic techniques. the metabolic clearance rate of progesterone from blood was 3-13 plus or minus 0-35 (s.e.m.) 1/min in ten experiments on six goats; values tended to be slightly higher in pregnant than in non-pregnant goats. the production rate of progesterone at oestrus, and at day 3 of the n ... | 1975 | 124325 |
| immunofluorescent localization of glycogenolytic and glycolytic enzyme proteins and of malate dehydrogenase isozymes in cross-striated skeletal muscle and heart of the rabbit. | specific antisera against glycogen phosphorylase, phosphofructokinase, aldolase, glyceraldehyde-phosphate dehydrogenase, enolase, lactate dehydrogenase, cytosolic and mitochondrial malate dehydrogenase from rabbit muscle were obtained from sheep. the gamma-globulins were used for indirect immunofluorescent localization of the respective enzymes in rabbit skeletal muscle and heart. in stretched skeletal muscle a cross-striation like distribution was observed for all enzymes studied. in the case o ... | 1975 | 124716 |
| effect of zinc and cadmium on delta-aminolevulinate dehydratase of red blood cells in protecting against enzyme losses during storage. | the effect of zinc and cadmium on delta-aminolevulinate dehydratase of bovine erythrocytes stored at -30 degrees c for different times was determined. the results show a. storage of erythrocytes leads to an enhancement of the enzyme activity, which after six weeks is 165% (500 mum zncl2) respectively 220% (100 mum cdcl2) for red blood cells of calves, and after four weeks in 420% respectively 450% (same concentrations) for red blood cells of adult cattle, b. the older the samples are, the higher ... | 1975 | 126587 |
| tightness and orientation of vesicles from guinea-pig kidney estimated from reactions of adenosine triphosphatase dependent on sodium and potassium ions. | in order to study the "sidedness" of the ligands of the na+, k+-atpase in the phosphorylation from [32p]atp, tight vesicles were prepared from guinea pig kidney and partially purified by a two-stage sucrose and ficoll gradient centrifugation procedure. these vesicles were derived presumably from plasma membrane fragments resealed after the initial disruption of the cells during homogenization. tightness of the vesicles was estimated according to activation by the nonionic detergent, triton x-100 ... | 1975 | 126864 |
| proceedings: observations on fragment d species arising during hydrolysis of crosslinked human fibrin. | | 1975 | 127400 |
| direct and utilization of metabolic energy for transport processes within proximal convolution. | | 1975 | 127689 |
| 'immobile' (im), a recessive lethal mutation of xenopus laevis tadpoles. | 'immobile' (im) is a recessive lethal mutation discovered in the f3 of a xenopus (xenopus laevis laevis) originating from a mesodermal nucleus of a neurula transplanted into an enucleated egg. the im embryos do not contract after mechanical stimulation nor do they present any spontaneous contraction from the neurula stage onwards. development proceeds normally during the first days after which deformation of the lower jaw and tail are observed. the im tadpoles die when normal controls are at the ... | 1975 | 127824 |
| [histochemical and quantitative studies of rna in the thyroid epithelium under the influence of thyrotropic hormone (tsh)]. | | 1975 | 127942 |
| [some properties of preparations of na+ and k+-atpase treated with deoxycholate]. | the treatment of membrane suspension from the guinea pig kidney cortex possessing na+, + k+ -atpase activity with 0.20% sodium deoxycholate (dox : protein equals 12 : 1) leads to lowering of their enzymatic activity. dox-treated preparation can be divided into two fractions by centrigugation (1 hr - 22 000 g). the pellet has 1.5-fold higher enzymatic activity than the initial preparation and cannot be activated by small amounts of histones. the supernatant has 2-fold lower enzymatic activity tha ... | 1975 | 128167 |
| allergic reaction to allopurinol with cross-reactivity to oxypurinol. | a 25-year-old white man with gout and nephropathy and with a previous reaction to allopurinol was given a trial dose of oxypurinol. he developed malaise, a generalized erythematous reaction with edema, pruritus, and emesis; this was clinically identical to the reaction he experienced with allopurinol. when the patient's lymphocytes were exposed in vitro to oxypurinol and allopurinol, increased dna synthesis was observed, suggesting an immunologic basis for the reaction. this patient indicates th ... | 1976 | 134655 |
| [prenatal development of monoamine neurons in the rat]. | the histofluorescence method allows the study of the prenatal development of monoamines neurons in the foetal rat brain. certain patterns of this development can be defined: the locus niger and the raphe appears fluorescent on the 14th day, the locus coeruleus on the 15th day. these systems differentiate independently in various areas of the neural axis. their maturation are progressive: first for the da system, then for the na and the 5 ht systems. the mechanisms for synthesis and storage of am ... | 1976 | 134781 |
| [influence of microflora on the metabolism of l-threonine at the level of the rat digestive tract]. | intestinal tissues are found to incorporate l threonine 14cu at faster rate in the absence of microflora. most of the radioactivity of the digestive contents occurs in the tca soluble compounds, except in the coecum and large intestine of the conventional rats where the microflora synthetized its own proteins from the labelled digestive material. free threonine is the main labelled soluble compound in the conventional rats digestive contents. in the other hand, in the germfree rats digestive con ... | 1976 | 134804 |
| localization on mitochondrial dna of mutations leading to a loss of rutamycin-sensitive adenosine triphosphatase. | four cytoplasmic mutants of saccharomyces cerevisiae showing loss of mitochondrial rutamycin-sensitive atpase activity but having significant cytochrome oxidase and nadh-cytochrome c reductase have been isolated. genetic studies indicate the mutations to be closely linked to each other and have been assigned to a new locus, pho1. the mutations show a low frequency of recombination with the ol12 locus, suggesting a linkage to this marker. they are not, however, linked to the oli1 locus. linkage o ... | 1976 | 134892 |
| the acid mucopolysaccharides (glycosaminoglycans) in secondary cultures of embryonic rat fibroblasts. | in secondary cultures of embryonic rat fibroblasts the protein-free acid mucopolysaccharides were separated by cetylpyridinium chloride fractionation and identified afterwards. the following acid mucopolysaccharides were found: hyaluronic acid, chondroitin, 2 heparan sulphate fractions, chondroitin-4-sulphate, chondroitin-6-sulphate, and dermatan sulphate. | 1975 | 134903 |
| biochemical variations in the matrix of human tendons in relation to age and pathological conditions. | with increasing age, the human tendon shows an increase of collagen and a diminution of mucopolysaccharides and glycoproteins. similar findings have recently been reported by other authors in the tendons of mammals. fragments of tendon affected by tendinosis, obtained dlring suture and tenoplasty operations, were also examined. marked diminution of collagen and an increase of acid mucopolysaccharides and structural glycoproteins were found as compared with the control tendon. | 1975 | 134980 |
| [value of a therapeutic combination: vitamin a-yeast-sulfur]. | | 1976 | 135073 |
| transplantation of hymenolepis diminuta into naive, immune and irradiated mice. | almost 100% of 7- to 10-day-old hymenolepis diminuta became established when surgically transplanted from donor mice into the duodenum of naive recipient mice. transplanted tapeworms survived 8-12 days, by which time they had survived much longer in total than they would have done in the donor. mice previously immunized by a primary infection rejected transplants within 4 days. sub-lethal irradiation (550 rad) delayed rejection by immune mice but such mice still rejected worms much more quickly ... | 1976 | 135242 |
| suppressor mechanisms in neonatally acquired tolerance to a gross virus-induced lymphoma in rats. | tolerance to a highly immunogenic gross virus-induced tumour in wistar/furth rats (c58nt)d was produced by neonatal infection of the rats with the virus. these rats failed to reject the tumours when challenged 8 weeks after virus inoculation and to mount the appropriate cell-mediated immune response to the tumour. the mechanisms involved were studied in vivo by adoptive transfers into sub-lethally irradiated rats of tumour cells mixed with spleen cells and/or sera from normal, tolerant, or tumou ... | 1976 | 135385 |
| [demonstration of the variations in accessibility or reactivity of mitochondrial sh groups according to the energy status of the mitochondria]. | evidence for energy dependent variations of mitochondrial sh groups accessibility or reactivity was obtained using alkylating agents. these variations are thought to be consecutive to conformational changes affecting some proteins among them the phosphate carrier and the adenylic nucleotide translocator. | 1977 | 145307 |
| variation in potassium transport properties of mouse 3t3 cells as a result of subcultivation. | unindirectional potassium influx and the fraction of this influx sensitive to ouabain, an inhibitor of the (na + k) activated atpase, have been evaluated as a function of subcultivation of the 3t3 and sv40 transformed 3t3 cell. total and ouabain-sensitive k influx change little over approximately 50 passages of the transformed 3t3 cell. in contrast, these components of k influx increase nearly 5-fold over a similar number of passages of the 3t3 cell. during early passages total and ouabain-sens ... | 1977 | 145445 |
| a role for central serotonergic systems in the pattern and intensity of the convulsive response of rats to electroshock. | | 1977 | 145548 |
| [effect of sh-reagents on atpase systems of rabbit skeletal muscle nuclei]. | the influence of sulfhydryl reagents on atpase systems of rabbit sceletal muscles nuclei was studied. it is found that p-chmb at low concentration similarly inhibits both mg2+- and mg2+, ca2+-atpases. p-chmb at higher concentrations inhibits completely mg2+, ca2+-atpase, while mg2+- atpase--only by 60%. n-em is lesser specific inhibitor of sh-groups, than p-chmb. the degree of nuclear atpases inhibition by n-em is practically identical. using inhibitory analysis, two hypes of skeletal muscles nu ... | 1978 | 149567 |
| [characteristics of prevalence of anthrax under the landscape-geographical conditions of the ivano-frankovsk district]. | | 1978 | 151465 |
| a cell line derived from bbn (n-butyl-n-[4-hydroxybutyl]-nitrosamine)-induced rat bladder cancer: establishment and scanning electron microscopic cell surface characteristics. | this research was performed to establish a cell line from experimental bladder tumor and to discuss the biological characteristics of the cell line so established. tissue cultures of epithelial cells were derived from a rat bladder cancer induced by bbn. the cells showed loss of contact inhibition and the phenomenon of piling up after several subcultures. colonial cloning was used. the population doubling time of the wild strain and the colonial clones was about 30 h. the chromosomal mode ranged ... | 1978 | 151485 |
| the effect of hypervitaminosis a on rat palatal development. | retinoic acid or retinyl acetate was administered to pregnant rats in doses sufficient to induce a 90% incidence of cleft palate. in another study, a delay in the reorientation of the palatal shelves was observed to be longer with the more potent teratogen, retinoic acid. on day 16 of gestation, 24 hours after final dosage with vitamin a, the synthesis of dna and protein was studied in fetal carcass, mandible, and palate, and that of sulfated mucopolysaccharides (s-mps) and glycoproteins (gp) in ... | 1978 | 152485 |
| the suppression of mitogen- and alloantigen-stimulated peripheral blood lymphocytes by cultured human t lymphocytes. | | 1979 | 156232 |
| patterns of neuromuscular disease. as related to stages of normal embryogenesis in voluntary muscle. | skeletal muscle samples from the upper and lower extremities of 38 human fetuses (6 to 18 weeks' gestation) of both sexes were studied by histologic, histochemical, and electron microscopic methods. ultrastructural morphometry was applied. in the different stages of normal development are found regressive changes, ranging from reversible dystrophic to irreversible necrotic alterations, which are characteristic of either primary myogenic myopathies or primary neurogenic muscle atrophies in older ... | 1979 | 156504 |
| isolation and characterization of mutants of haemophilus influenzae deficient in an adenosine 5'-triphosphate-dependent deoxyribonuclease activity. | by a direct assay approach, mutants of haemophilus influenzae rd that are deficient in adenosine 5'-triphosphate-dependent deoxyribonuclease activity (add-) were isolated and characterized. a large proportion (50 to 90%) of the cells in cultures of these mutants failed to produce visible colonies when plated. an extensive analysis of the recombination proficiency of these strains revealed that the transformation frequency (transformants per competent cell) in the mutants was similar to that foun ... | 1975 | 165169 |
| a simple radioimmunoassay for 3',5', cyclic adenosine monophosphate. | | 1975 | 165745 |
| thymosin-induced differentiation of murine thymocytes in allogeneic mixed lymphocyte cultures. | calf thymosin is shown to enhance the one-way mlr of cba thymocytes cultured with allogeneic mitomycin-c- treated c57bl/j6 spleen cells. thymosin does not enhance the one-way mlr of cba thymocytes cultured with syngeneic mitomucin-c-treated spleen cells. based on this finding we present a relatively simple, rapid and quantitative in vitro microculture hioassay for inducers of t-cell differentiation and propose that thymosin treatment, when accompanied by antigen presentation, induces the two-ste ... | 1975 | 165764 |
| anti-phosphodiesterase activity of the coronary dilating agent hydrochloride carbocromene. | | 1975 | 167386 |
| nuclease digestion of the dna-protein complex from simian virus 40 virions. | | 1975 | 167841 |
| proceedings: variations in cgmp and camp levels in rat uterine smooth muscle induced by carbachol, pgf2alpha and changes in ionic composition. | | 1975 | 167895 |
| influence of atropine, metiamide and vagotomy on camp of resting and stimulated gastric mucosa. | in normal rats the effect of atropine and metiamide was studied on camp levels of resting and histamine-, pentagastrin-, carbachol- and insulin-stimulated gastric mucosa. in vagotomized rats gastric mucosal camp levels were investigated under basal conditions and after insulin. atropine and metiamide did not alter camp levels of resting gastric mucosa. truncal vagotomy caused an increase in gastric mucosal camp, which was not affected by insulin. all gastric secretagogues caused a significant ri ... | 1975 | 168089 |
| immunological relationship between delta herpesvirus of patas monkeys and varicells-zoster virus of humans. | the delta herpesvirus (dhv) which produced outbreaks of exanthematous disease in patas monkeys was shown to have a close immunological relationship to varicella-zoster (v-z) virus of man. immunization of rhesus monkeys with dhv or v-z virus resulted in the development of neutralizing antibodies to both viruses and also in the production of complement-fixing antibodies to v-z virus. immunoglobulin m neutralizing antibody to v-z virus was demonstrated in the serum of a rhesus monkey immunized with ... | 1975 | 168152 |
| survival of human enteric and other sewage microorganisms under simulated deep-sea conditions. | the survival of pure cultures of escherichia coli, streptococcus faecalis, clostridium perfringens, and vibrio parahaemolyticus under simulated deep-sea conditions of low temperature (4 c), seawater, and hydrostatic pressures ranging from 1 to 1,000 atm was determined over a period exceeding 300 h. the viability of e. coli and total aerobic bacteria in seawater-diluted raw sewage subjected to these deep-sea conditions was also measured. there was a greater survival of both e. coli and s. faecali ... | 1975 | 169733 |
| inheritance of a lethal immunodeficiency disease of arabian foals. | a fatal syndrome of certain arabian foals which begins at about 25 days of age (range 14 to 46 days) and which runs a course of about 23 days (range 13 to 42 days) is described. the syndrome, which affected 17 foals on a single farm is further characterised by pneumonia, in some instances by dermatitis (dermatophilosis) and other infections, together with a progressive decline in health till death at about 49 days of age (range 34 to 77 days), despite intensive therapy. four of the foals, on his ... | 1975 | 169784 |
| mutants defective in binding activity for cyclic adenosine 3',5'-monophosphate in vibrio parahaemolyticus. | mutants of vibrio parahaemolyticus defective in binding activity for adenosine 3',5'-cyclic monophosphate are described. they were selected with medium containing the nucleotide together with starch. | 1975 | 170251 |
| hepatic metabolism of pentites. | | 1975 | 170566 |
| [selective isolation of a crp (cyclic amp receptor protein)-defective mutant by growth suppression by cyclic amp]. | | 1975 | 175181 |
| immune host response to corneal grafts sensitized to herpes simplex virus. | experiments were performed to study the fate of corneal grafts sensitized to herpes antigens when grafted to hsv-sensitized hosts. nonsensitized grafts in systemically sensitized hosts remained clear, whereas all locally sensitized grafts in systemically sensitized hosts became opaque within ten days. the stroma showed severe lymphocytic and plasma cell infiltration and neovascularization. one-half of the corneas from systemically sensitized hosts grafted to eyes previously infected opacified in ... | 1976 | 176129 |
| foot-and-mouth disease virus rna. presence of 3'-terminal polyriboadenylic acid and absence of amino acid binding ability. | | 1976 | 176778 |
| temperature-dependent inactivating factor of pseudomonas aeruginosa exotoxin a. | the adenosine diphosphate ribosyl transferase activity of pseudomonas aeruginosa exotoxin a(pa toxin) was found to be rapidly destroyed by heating at 45 to 60c but not by heating at 70 to 90c (for at least 30 min). this phenomenon has been previously described for other bacterial toxins (staphylococcal alpha-toxin and vibrio parahaemolyticus hemolysin) and is termed an arrhenius effect. in contrast, the arrhenius effect was not seen when the pa toxin was heat-treated as above and tested for cell ... | 1976 | 178606 |
| developmental changes in mitochondria during the transition into lactation in the mouse mammary gland. ii. membrane marker enzymes and membrane ultrastructure. | the activity of cytochrome oxidase (an inner mitochondrial membrane marker) in mouse mammary gland homogenates was found to increase five- to sixfold from late pregnancy to day 8 of lactation, while that of monoamine oxidase (an outer membrane marker) increased only about 25%. the specific activity of cytochrome oxidase in the isolated mitochondria decreased slightly over the same period while the specific activity of monoamine oxidase decreased fivefold. this reflects the fact that both cytochr ... | 1976 | 178667 |
| characterizatiion of rat genetic sequences of kirsten sarcoma virus: distinct class of endogenous rat type c viral sequences. | the nucleic acid sequences found in dna and rna from rat cells which are homologous to kirsten sarcoma virus have been characterized. the homologous sequences are present in multiple copies per diploid rat cellular genome in a variety of different rat cellular dna's. in certain cells that constitutively express only low levels of sequences homologous to kirsten sarcoma virus, bromodeoxyuridine treatment leads to the expression of high levels of these sequences in rna. supernatants from cell line ... | 1976 | 178897 |
| [localization of genome rna synthesis in sendai virus]. | localization of sendai virus 50s rna in ehrlich ascitic carcinoma cells was studied. at 48 hours postinfection virus-specific 50s rna was found in the nucleus after 30 min exposure to 3h-uridine, and its amount increased after 1-hour exposure to the precursor reaching 18% of the total nucleus virus-specific rna. after 2-hour or longer exposure to the precursor the amount of 50s rna in the nuclei decreased considerably and in the cytoplasm in this gradient region a considerable radioactivity appe ... | 1975 | 179216 |
| laboratory investigation of diarrhea in travelers to mexico: evaluation of methods for detecting enterotoxigenic echerichia coli. | a laboratory investigation was conducted on cultures collected from travelers before, during, and after a trip to mexico to characterize the etiology of traveler's diarrhea. four laboratory methods for detecting enterotoxigenicity of escherichia coli were evaluated: the infant mouse assay, the chinese hamster ovary (cho) cell assay, the y1 adrenal cell assay, and the rabbit ileal loop. although a number of common enteric pathogens were identified as a cause of traveler's diarrhea, including six ... | 1976 | 180047 |
| nitrogenase of azotobacter chroococcum. kinetics of the reduction of oxidized iron-protein by sodium dithionite. | the kinetics of the reduction of oxidized fe-protein of nitrogenase from azotobacter chroococcum by sodium dithionite were studied by stopped-flow and rapid-freezing e.p.r. (electron-paramagnetic-resonance) spectroscopy. the appearance of the gav. = 1.94 e.p.r. signal (0.24 electron integrated intensity/mol) was associated with a one-electron reduction by so2--with k greater than 10(8)m-1-s-1 at 23 degrees c. a value of k = 1.75s-1 was obtained for the rate of dissociation of s2o42- into 2so2-- ... | 1976 | 180978 |
| how the mouse leukemia virus was discovered. a talk with ludwik gross. | | 1976 | 184434 |
| laboratory diagnosis of foodborne diseases. | many bacterial species are responsible for sporadic cases and outbreaks of foodborne intoxication and infection. the foodborne diseases are classified on the basis of the pathogenetic mechanisms involved into four categories: performed toxin, enterotoxin formed in the colonized small intestine, mucosal invasion (enterocolitis) and mucosal invasion with bacteremia. invasive and toxigenic strains of enteropathogenic escherichia coli are discussed. in vivo test systems for the identification of ent ... | 1976 | 184732 |
| quantification of human atrioventricular nodal concealed conduction utilizing s1s2s3 stimulation. | we studied antegrade concealed conduction of atrial extrastimuli (a2) that blocked in the atrioventricular (av) node in eight subjects, using a third extrastimulus (a3), coupled at decreasing coupling intervals to a2. three a1-a2 intervals were tested in each subject: late (just shorter than av nodal effective refractory period), intermediate, and early (just longer than atrial functional refractory period). the curves relating the following variables were constructed for each a2: a1-a3, h1-h3 a ... | 1976 | 184975 |
| reconstitution of t cell function in patients with subacute sclerosing panencephalitis treated with thymus humoral factor. | two patients with subacute sclerosing panencephalitis (sspe) showed impairment of cell-mediated immunity, as indicated by a low t cell number, decreased intracellular cyclic amp levels of peripheral blood lymphocytes, negative graft-vs.-host reaction in vivo, negative skin reactions to common antigens and, in one of the patients, abnormal reactions in migration inhibition factor tests. since some of the impaired t cell functions in one of the patients were reconstituted in vitro by the administr ... | 1976 | 186082 |
| [carbohydrate metabolism in the kidneys of rats with dimethylnitrosamine-induced tumors]. | under study was anaerobic glycolysis, the activity of hexokinase, lactate dehydrogenase, gluco-6-phosphatase in rat kidneys in dimethylnitrosamine (dmna) induced tumors in them. dmna was administered perorally in the dosage of 10 mg/kg during 4 weeks (25 mg per rat). following 8 months tumors developed in the renal cortical substance. the tumor tissue, the renal cortical substance adjacent to the tumor, and the renal cortical substance without macroscopic tumor signs have been studied. an increa ... | 1976 | 186957 |
| [histochemical studies on the role of myoepithelial cells in the morphogenesis of mammary tumors in the bitch. 3. immunohistochemical findings in mammary tumors]. | | 1976 | 186986 |
| the nadph oxidase activity of polymorphonuclear neutrophilic leukocytes revisited. | | 1976 | 187027 |
| inhibitory effects of prostaglandin e1 on responses of rabbit ear artery to nerve stimulation and on release of norepinephrine. | pge1 in concentrations of 7 x 10(-9) to 4.5 x 10(-7) m inhibited the vasoconstrictor responses of the perfused rabbit ear artery to nerve stimulation. the degree of inhibition was dependent on the concentration of pge1, as well as on the train length and frequency of stimulation. the vasoconstriction produced by norepinephrine was not altered by 1.1 x 10(-7) m pge1. this substance markedly reduced the stimulation-evoked outflow of 3h-norepinephrine from the artery. the findings indicate that pge ... | 1976 | 187049 |
| reversal of the memory-disruptive effects of rem sleep deprivation by physostigmine. | | 1976 | 187162 |
| the action of snake venom, phospholipase a and trypsin on purified myelin in vitro. | 1. purified myelin was incubated with snake venom or phospholipase a in the presence of or absence of trypsin at 37 degrees c, ph7.4, for different times. 2. analysis of the myelin pellet obtained after centrifugation of the myelin sample incubated with snake venom or phospholipase a alone showed conversion of phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine into their corresponding lyso compounds. no significant loss of myelin protein was observed in these samples. 3. a mark ... | 1976 | 187172 |
| a progressive approach to the problem of foodborne infections. | qualitative and quantitative bacteriological examinations of 100 samples of perishable foods from 39 retail stores were performed to determine the presence of bacterial contaminants and to explore the feasibility of establishing and utilizing microbiological standards in enforcement. forty-six per cent of the samples had standard plate counts in excess of 100,000 per gram, 17 per cent showed coliform organisms in excess of 100 per gram, 20 per cent revealed the presence of staphylococcus aureus ... | 1977 | 188346 |
| infectious murine type-c viruses released from human cancer cells transplated into nude mice. | type-c virus particles were revealed by electron microscope in 6 of 9 tumours of cultured and biopsied human cancers heterotransplanted into nude mice. some tumours in nude mice were explanted for in vitro cultivation. the virus particles were also found in the cultures derived from the virus-positive tumors. they were mostly found extracellularly, but the particles in budding process were also encountered frequently. cytological study and karyotype analysis of the cultured cells proved these vi ... | 1977 | 193756 |
| the storage lipids in tangier disease. a physical chemical study. | the physical states and phase behavior of the lipids of the spleen, liver, and splenic artery from a 38-yr-old man with tangier disease were studied. many intracellular lipid droplets in the smectic liquid crystalline state were identified by polarizing microscopy in macrophages in both the spleen and liver, but not in the splenic artery. the droplets within individual cells melted sharply over a narrow temperature range, indicating a uniform lipid composition of the droplets of each cell. howev ... | 1977 | 193870 |
| [nuclear receptors for triiodothyronine. i. binding of triiodothyronine (t-3) in rat liver following in vivo administration of labelled hormone]. | | 1977 | 195802 |
| preparation of biologically active tritium-labelled 1--34 human parathyroid hormone. | | 1977 | 195840 |
| cellular metabolic control by chemical modification of cell membrane. | various reagents used in the chemical modification of amino- and carboxy-groups of proteins, and of carbohydrates of glycoproteins and glycolipids, inhibit respiration in ascites tumor cells concomitant with release of potassium ion from those cells. the respiratory activity of washed ascites tumor cells is increased by exogenous addition of potassium ion. the lowered respiratory control index as well as oxidative phosphorylation of aged mitochondria are restored upon increasing the potassium co ... | 1976 | 196300 |
| etiology of virus pneumonia among children in peking, 1973-1975. | | 1977 | 196816 |
| bacterial hazards. | | 1977 | 197534 |
| progesterone interaction with estrogen and antiestrogen in the rat uterus--receptor effects. | daily injections of estradiol or the antiestrogen tamoxifen initially stimulate uterine weight increase and progesterone receptor synthesis, though continued tamoxifen fails to maintain the increased weight. the stimulatory actions of both estradiol and tamoxifen are inhibited or reversed by a single injection of progesterone. it has been hypothesized that progesterone antagonizes estrogen action by reducing estrogen receptor levels, but in the present experiments neither cytoplasmic nor nuclear ... | 1977 | 201053 |
| plasma adrenocorticotropin levels in normal dogs. | plasma concentrations of adrenocorticotropin (acth; corticotropin) were measured in 31 normal dogs (house pets) at rest. the mean concentration was 45.77 pg/ml of blood, with individual values ranging from 17 to 98 pg/ml of blood. the measurement of this hormone secreted by the anterior lobe of the pituitary gland (adenohypophysis) may aid in the diagnosis of spontaneous canine adrenal cortical disorders, both primary and secondary. | 1977 | 201191 |
| an investigation of the homology of guanylate kinase isozymes in mammals and further evidence for multiple guk gene loci. | guanylate kinase in the red cells of 63 different mammalian species was studied by electrophoresis and multiple molecular forms of the enzyme were found in all species. two species were investigated in more detail. using molecular weight estimates as a criterion of homology, the fallow deer and the chinese hamster were found to have isozymes that corresponded to isozyme e, f, and g of man. variation in the guanylate kinase isozymes was detected in a small population of orangutans. results sugges ... | 1977 | 201245 |
| 1,2,4-triazole amino nucleosides. 1-beta-d-3'-amino-3'-deoxyribofuranosyl-1,2,4-triazole-3-carboxamide and related nucleosides. | the synthesis of 1-beta-d-3'-amino-3'-deoxyribofuranosyl-1,2,4-triazole-3-carboxamide--the 3'-amino analogue of ribavirin--and five related nucleoside analogues is described. each analogue exhibited ld50 concentrations greater than 100 microgram/ml against p-388 mouse lymphoid leukemia cells in tissue culture. antiviral testing indicated that none of the compounds exhibited significant activity. | 1977 | 201758 |
| electron paramagnetic resonance spectra of myeloperoxidase in polymorphonuclear leukocytes. | | 1978 | 202508 |
| [effect of various antibiotics, disinfectants and uv light on the viability of vegetative cells and spores of cl. perfringens type a]. | | 1977 | 202819 |
| replication of vesicular stomatitis virus facilitated in nonpermissive cells by early functions of shope fibroma virus. | | 1978 | 202973 |
| [new findings on various bacteria and their toxins as possible causative agents of food poisoning]. | | 1977 | 203102 |
| calcium gradient dependent pyrophosphate formation by sarcoplasmic vesicles. | the vesicles of the sarcoplasmic membranes synthesize pyrophosphate from inorganic phosphate. pyrophosphate synthesis proceeds as long as a calcium gradient is maintained across the vesicular membranes. pyrophosphate synthesis is inhibited by low concentrations of nucleoside triphosphates. | 1977 | 203132 |
| changes of airway smooth muscle in experimental asthma. | | 1978 | 203991 |
| heterophil-negative infectious mononucleosis and mononucleosis-like illnesses. laboratory confirmation of 43 cases. | | 1977 | 204189 |
| mitogen induction of murine c-type viruses. iv. effects of lipoprotein e. coli, pokeweed mitogen and dextran sulphate. | lipoprotein e. coli, a b-cell mitogen, is identified as a new agent inducing the release of endogenous c-type virus from mouse spleen cells. like lipopolysaccharide, a previously identified inducer, this compound has a synergistic effect with 5-bromo-2'-deoxyuridine. induced virus has the characteristic density as well as morphology of c-type viruses. budding viruses are detected on cultured balb/c cells by electron microscopy 2 to 4 days following culturing in the presence of lipoprotein. pokew ... | 1978 | 204733 |
| latex agglutination test for amoebiasis in pakistani patients with chronic 'obscure' liver enlargements. | the latex agglutination test for amoebiasis was done in 50 pakistani patients in whom a clinical diagnosis of chronic non-supparative amoebic hepatitis was made on the basis of liver enlargement associated with chronic recurrent abdominal disturbance. the serological test was negative in 32 (64%) and positive in 18 (36%). examination of stool specimens showed a higher frequency of e. histolytica in serology positive patients. the liver biopsy findings were variable and included normal histology ... | 1978 | 204801 |
| the virion and soluble antigen proteins of variola, monkeypox, and vaccinia viruses. | the structural proteins in purified preparations of variola, monkeypox, and vaccinia viruses were separated and compared by using a high resolution sds-polyacrylamide gel electrophoresis system. about 30 proteins were resolved for each virus by autoradiography of longitudinally-sliced gel rods. although the autoradioelectropherograms of each virus were similar, it was possible to differentiate them by their unique protein pattern in the 30,000 to 40,000 molecular weight region of the gels. a sin ... | 1977 | 205632 |
| structure of human hemoglobin messenger rna and its relation to hemoglobinopathies. | 1. one-fifth to 1/4 of globin mrna is untranslated sequence other than polyadenylic acid. 2. the untranslated sequences of mrna vary markedly in their sequence and in their length. 3. globin mrnas demonstrate a marked bias in codon selection. 4. viral mrna shows a quite different pattern of codon selection; therefore, the selection of codons is not uniform for all mrnas functioning in animal cells. 5. elongated hemoglobin chains can be accounted for by frame-shift mutations, or point mutations w ... | 1977 | 205979 |
| demonstration of two different types of beta1-receptors in man (selective blockade of the positively inotropic and the positively chronotropic effect of isoproterenol). | the hemodynamic effect of a beta-receptor blockade with mepindolol--a noncardioselective beta-receptor blocker--was studied in 6 male test subjects age 25 to 30 years with an increasing dose of isoproterenol. the cardiac output per min, the heart rate and the stroke volume, the wet blood pressure and the contractility parameters dp/dt max in the right and left ventricles were measured as part of the study. it was found that a dissociated right shift of the dose-effect curves occurred for the str ... | 1978 | 208993 |
| [biochemical features of the liver of high and low cancer mouse strains and primary spontaneous hepatomas]. | sex differences in development of spontaneous liver tumors were studied in mice of cba strain. content of mitochondrial proteins, activities of cytochrome oxidase, glucokinase, hexokinase as well as subcellular distribution of hexokinase were also studied in primary hepatomas and in normal liver tissue of the mice of cba and c3ha strains. liver tumors occurred in males of cba strain 7-fold more frequently than in females. activities of hexokinase, glucokinase, cytochrome oxidase and content of m ... | 1978 | 210588 |
| evidence for the presynaptic location of the alpha-adrenoceptors which regulate noradrenaline release in the rat submaxillary gland. | | 1978 | 211446 |
| fructose-1,6-biphosphatase of the small intestine. purification and comparison with liver and muscle fructose-1,6-bisphosphatases. | the occurrence of specific fructose-1,6-bisphosphatase [d-fructose-1,6-bisphosphate 1-phosphohydrolase, ec 3.1.3.11] (fru-1,6-p2ase) in the small intestine was confirmed. 1. fru-1,6-p2ase was isolated from mouse small intestine by a simple method. the isolated enzyme preparation was an electrophoretically homogeneous protein. 2. the molecular weight and subunit molecular weight were 140,000 and 38,000, respectively. 3. the intestinal enzyme was electrophoretically distinct from the liver enzyme. ... | 1978 | 212415 |
| comparison of interferon action in interferon resistant and sensitive l1210 cells. | translation inhibition, leu-trna aminoacylation and double-stranded rna and atp dependent phosphorylation were examined in interferon-treated and control cell-free lysates of leukaemic mouse l 1210 r and l 1210 s cells. no differences were observed between the respective interferon-treated and control cell-free extracts, except for the presence of an enhanced 67k dalton phosphoprotein fraction in interferon-treated l 1210 s cell-free extracts. in non-responding cell-free lysates, the lack of sti ... | 1978 | 212514 |