| deficiencies of chromatophore proteins in some mutants of rhodopseudomonas spheroides with altered carotenoids. | chromatophore proteins of a wild type and three mutant strains of rhodopseudomonas spheroides were examined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. the mutants consisted of a green and a blue-green one, whose phenotypes were essentially the same as those of known mutants, and a brown one, which may be a double mutant and represents a new phenotype. wild-type chromatophores contained at least six major and seven minor protein bands, with molecular weights ... | 1971 | 4323296 |
| avian leukosis-sarcoma virus antibodies in wildfowl, domestic chickens and man in kenya. | | 1973 | 4358887 |
| the "nicotinic" and "muscarinic" receptors of the urinary bladder of the guinea-pig. | | 1966 | 4381813 |
| [a study of fructose metabolism in the human liver]. | | 1971 | 4399035 |
| ovarian activity and peripheral plasma levels of oestrogens and progesterone in the lactating sow. | | 1974 | 4468919 |
| effect of oral chenodeoxycholic acid on bile acid kinetics and biliary lipid composition in women with cholelithiasis. | bile acid kinetics and biliary lipid composition were characterized in six women with gallstones before and after 6 mo of oral therapy with chenodeoxycholic acid, an agent that induces dissolution of cholesterol gallstones in man. over a dosage range of 1-4 g/day, absorption varied from 0.8 to 2.3 g/day. the chenodeoxycholic acid pool expanded two-to sixfold, and bile became composed predominantly (> 90%) of chenodeoxycholic acid conjugated chiefly with glycine. cholic acid and deoxycholic acid ... | 1973 | 4583981 |
| hypertension as a localizing sign of mass lesions of brainstem. | | 1972 | 4635025 |
| oxidative drug metabolism in liver microsomes from uremic rats. | | 1972 | 4669451 |
| retinol glycolipids. | | 1973 | 4684696 |
| [comparative studies on cerebral vessel pathology in animals and man]. | | 1973 | 4725275 |
| the metabolism of 5alpha-dihydrotestosterone and androsterone by skin. | | 1973 | 4802629 |
| reversible damage of rat upper respiratory tract caused by cigarette smoke. | | 1974 | 4809936 |
| the hydroxylation of d-amphetamine by liver microsomes of the male rat. | | 1974 | 4824906 |
| the role of heme in the release of iron from transferrin in reticulocytes. | | 1974 | 4836430 |
| [influence of skf 525a on the kinetics and action of an antivitamin k in the rat]. | | 1974 | 4848952 |
| [survey of entero-infections in urban and rural areas of the algerois]. | | 1970 | 4950343 |
| [survey of entero-infections in urban and rural areas of the algerois]. | | 1970 | 4950343 |
| demonstration of human reagin in monkey tissues. 3. in vitro passive sensitization of monkey ileum with sera of atopic patients. physiologic and enhancing experiments. | | 1967 | 4961409 |
| physical, chemical, and serological studies on carnation mottle virus. | | 1970 | 4991706 |
| a simple and rapid method for quantitative determination of protein in urine. | | 1972 | 5061737 |
| electron microscopic examination of factors influencing the expression of filamentous surface structures on clinical and environmental isolates of aeromonas veronii biotype sobria. | strains of aeromonas veronii biotype sobria isolated from clinical and environmental sources were examined for their expression of surface structures under a variety of culture conditions. when grown on solid media at 37 degrees c, more than 95% of bacteria from the majority of strains isolated from human diarrheal feces and chicken carcasses were non-piliated or expressed only a few pili of long, flexible morphology per cell. strains isolated from water or other foods were much more likely to e ... | 1995 | 7565173 |
| a specific puld homolog is required for the secretion of paracrystalline surface array subunits in aeromonas hydrophila. | aeromonas hydrophila is an important pathogen of fish, and its high-virulence strains display a two-dimensional paracrystalline layer (s-layer) on their outermost surfaces. the nucleotide sequence of a 4.1-kb region located 700 bp upstream of the a. hydrophila tf7 s-layer protein gene (ahsa) has been determined. a sequence analysis of the region revealed the presence of three complete open reading frames ending in a gene encoding a 79.8-kda polypeptide that shows high homology to the puld family ... | 1995 | 7608063 |
| case of aeromonas veronii (dna group 10) bacteremia. | we describe the first case report of bacteremia due to aeromonas veronii biotype veronii. the infection occurred in a 77-year-old man suffering from multiple underlying conditions which included cancer of the sigmoid colon. because of the unusual biochemical phenotype of this group (ornithine decarboxylase positive), it was originally identified as vibrio cholerae. | 1994 | 7883912 |
| aeromonas species in septicemia: laboratory characteristics and clinical observations. | we retrospectively analyzed clinical and epidemiological data on and laboratory characteristics of 53 cases of aeromonas septicemia. only four aeromonas genomospecies (species defined by dna relatedness) were associated with the 53 cases, with aeromonas hydrophila (sensu stricto) predominating (47%). nearly 60% of all aeromonas isolates from blood fell into one of four somatic groups: serogroups o:11, o:16, o:18, and o:34. unlike aeromonas-associated gastroenteritis, septicemia did not peak in f ... | 1994 | 7948561 |
| the susceptibility of s-layer-positive and s-layer-negative aeromonas strains to complement-mediated lysis. | forty strains of aeromonas hydrophila and aeromonas veronii recovered from invasive and non-invasive infections were tested for their susceptibility to complement-mediated lysis by 65% pooled human serum (phs). based upon the results of this assay, two major populations could be defined. the first group (n = 20) consisted of serogroup o:11 strains, all of which possessed a paracrystalline surface layer (s layer); all of these strains were refractory to the bactericidal activity of 65% phs with t ... | 1994 | 8000552 |
| pili and the interaction of aeromonas species with human peripheral blood polymorphonuclear cells. | the interaction of differentially piliated aeromonas strains expressing pili of two broadly different morphologic types (short, rigid (s/r) and/or long, wavy (l/w)) with human peripheral blood mononuclear leukocytes (pmn) was investigated to determine whether host defense cells might exert a selective pressure on pili expression in vivo accounting for the different pili phenotypes of clinical and environmental strains. a majority of aeromonas veronii biotype sobria strains from water (6/6) and f ... | 1993 | 8104626 |
| meningitis due to aeromonas species: case report and review. | in recent years, aeromonas species has been reported to cause extraintestinal infections with a growing frequency. meningitis due to aeromonas species is, however, a rare entity. we report a case of aeromonas meningitis in a 54-year-old man with a history of chronic alcoholic liver disease who, after an episode of gastroenteritis, developed an acute clinical picture characteristic of meningitis with septic shock and ecthyma gangrenosum. aeromonas veronii (biogroup sobria) was isolated from cultu ... | 1993 | 8110931 |
| aeromonas enteropelogenes and aeromonas ichthiosmia are identical to aeromonas trota and aeromonas veronii, respectively, as revealed by small-subunit rrna sequence analysis. | the 16s rrna gene sequences of the type strains of aeromonas enteropelogenes and aeromonas ichthiosmia were determined by polymerase chain reaction direct sequencing in order to clarify their interrelationships with other aeromonad species. on the basis of 16s rrna gene sequence analysis, a. enteropelogenes and a. ichthiosmia were found to be identical to aeromonas trota and aeromonas veronii, respectively. | 1993 | 8240968 |
| adhesion of aeromonas sp. to cell lines used as models for intestinal adhesion. | adhesion to hep-2 cells has been shown to correlate with enteropathogenicity for aeromonas species. such adhesion is thought to reflect the ability of strains to adhere to human intestinal enterocytes, although hep-2 cells are not of intestinal origin. in this study strains of aeromonas veronii biotype sobria isolated from various sources were investigated in parallel assays for their ability to adhere to hep-2 cells and to an intestinal cell line (caco-2). quantitative assays showed identical a ... | 1995 | 8557078 |
| synergistic iron reduction and citrate dissimilation by shewanella alga and aeromonas veronii | two bacterial isolates from great bay estuary, new hampshire, in co-culture carried out anaerobic dissimilation of citric acid with fe(iii) as the terminal electron acceptor. neither isolate oxidized citrate with fe(iii) anaerobically in axenic culture. the fe(iii) reducer, shewanella alga strain bry, did not grow anaerobically with citrate as an energy source. the citrate utilizer, aeromonas veronii, did not reduce iron axenically with a variety of electron donors including citrate. the onset o ... | 1996 | 8824150 |
| characterization of a type iv bundle-forming pilus (sfp) from a gastroenteritis-associated strain of aeromonas veronii biovar sobria. | the colonization mechanisms of enteropathogenic aeromonas strains are poorly characterized, but recent studies indicate that some filamentous structures are intestinal adhesins. this study describes the purification and characterization of a long, flexible pilus from a gastroenteritis-associated strain of aeromonas veronii biovar sobria. sds-page analysis (various conditions) of pili preparations yielded a pilin protein band of approximately 21 kda. its n-terminal amino acid sequence was unambig ... | 1996 | 8827704 |
| high-resolution genotypic analysis of the genus aeromonas by aflp fingerprinting. | we investigated the ability of a recently developed genomic fingerprinting technique, named aflp, to differentiate the 14 currently defined dna hybridization groups (hgs) in the genus aeromonas. we also determined the taxonomic positions of the phenospecies aeromonas allosaccharophila, aeromonas encheleia, aeromonas enteropelogenes, and aeromonas ichthiosmia, which have not been assigned to hgs yet. a total of 98 aeromonas type and reference strains were included in this study. for the aflp anal ... | 1996 | 8934910 |
| identification and molecular characterization of two tandemly located flagellin genes from aeromonas salmonicida a449. | two tandemly located flagellin genes, flaa and flab, with 79% nucleotide sequence identity were identified in aeromonas salmonicida a449. the fla genes are conserved in typical and atypical strains of a. salmonicida, and they display significant divergence at the nucleotide level from the fla genes of the motile species aeromonas hydrophila and aeromonas veronii biotype sobria. flaa and flab encode unprocessed flagellins with predicted mrs of 32,351 and 32,056, respectively. when cloned under th ... | 1997 | 9286979 |
| distribution and expression of beta-lactamase genes among aeromonas spp. | clinical and environmental isolates of aeromonas spp. (20 aeromonas caviae, 33 aeromonas veronii and 21 aeromonas hydrophila) were examined for their ability to yield mutants derepressed for beta-lactamase production and for the distribution of the three chromosomally encoded beta-lactamase genes, amps and ceps from a. veronii bv. sobria and cpha from a. hydrophila. of these isolates, 100% and 96% of the isolates from a. hydrophila and a. caviae, respectively, yielded beta-lactamase derepressed ... | 1997 | 9301981 |
| indwelling device-related and recurrent infections due to aeromonas species. | from october 1995 to february 1997, 13 isolates of aeromonas species were recovered from four patients treated at national taiwan university hospital (taipei). one of the patients, a diabetic, had simultaneous aeromonas veronii biotype veronii bacteremia and a. veronii biotype sobria urinary tract infection. seven weeks after the episode, the patient had necrotizing fasciitis due to a. veronii biotype veronii. the other three patients all had underlying hepatobiliary malignancies complicated by ... | 1998 | 9524839 |
| nucleotide and amino acid sequences of the metallo-beta-lactamase, imis, from aeromonas veronii bv. sobria. | the aeromonas veronii bv. sobria metallo-beta-lactamase gene, imis, was cloned. the imis open reading frame extends for 762 bp and encodes a protein of 254 amino acids with a secreted modified protein of 227 amino acids and a predicted pi of 8.1. to confirm the predicted sequence, purified imis was digested and the resulting peptides were identified, yielding an identical sequence for imis, with 98% identity to cpha. both possessed the putative active-site sequence asn-tyr-his-thr-asp at positio ... | 1998 | 9527802 |
| misidentification of unusual aeromonas species as members of the genus vibrio: a continuing problem. | two unusual cases of aeromonas infection are described, one associated with bacteremia (aeromonas schubertii) and another in which the organism was recovered from an infected gall bladder (aeromonas veronii biotype veronii). these strains were initially identified as vibrio damsela and vibrio cholerae by the vitek and api 20e systems, respectively. use of appropriate screening tests and familiarity with the newer aeromonas species could prevent initial misidentifications and potential public hea ... | 1998 | 9542946 |
| phenotypic identification of aeromonas genomospecies from clinical and environmental sources. | a collection of 983 aeromonas isolates from environmental and clinical sources have been identified to the genomospecies level. a phenotypic method identified 93% of the strains. the use of citrate and the production of acid from sorbitol enabled the members of the aeromonas hydrophila complex to be separated. the most common genomospecies from intestinal sources were aeromonas veronii biotype sobria and aeromonas caviae. the former, together with a. hydrophila, was the most frequently isolated ... | 1998 | 9575026 |
| septic arthritis due to aeromonas veronii biotype sobria. | | 1998 | 9709899 |
| [new species of aeromonas isolated in cuba]. | one hundred and fifty-five strains of aeromonas isolated in the stools of children under 5 years presenting with acute diarrheal disease were studied. using the aerokey ii system for the identification of species, 47 strains were identified as aeromonas caviae, 58 as aeromonas hydrophila, 23 as aeromonas veronii biovar sobria, 14 as aeromonas trota, 9 as aeromonas veronii biovar veronii, 2 as aeromonas jandaei and 2 as aeromonas shubertii, emphasis is placed on the advantages of this method whic ... | 1995 | 9813480 |
| symbiosis of aeromonas veronii biovar sobria and hirudo medicinalis, the medicinal leech: a novel model for digestive tract associations. | hirudo medicinalis, the medicinal leech, is applied postoperatively in modern medicine. infections by aeromonas occur in up to 20% of patients unless a preemptive antibiotic treatment is administered. the associated infections demonstrate the need for a better understanding of the digestive tract flora of h. medicinalis. early studies reported the presence of a single bacterial species in the digestive tract and suggested that these bacteria were endosymbionts contributing to the digestion of bl ... | 1999 | 9864188 |
| the production of a novel carbapenem-hydrolysing beta-lactamase in aeromonas veronii biovar sobria, and its association with imipenem resistance. | | 1998 | 10052912 |
| prevalence, in-vitro secretory activity, and cytotoxicity of aeromonas species associated with childhood gastroenteritis in chennai (madras), india. | an investigation on the prevalence of aeromonas in gastrointestinal illnesses of pediatric inpatients 1 month to 3 years of age was conducted from february 1997 through january 1998 in madras. sixteen aeromonas spp. were isolated from 11 male and five female children among the 341 pediatric inpatients suffering from acute diarrhoea. a. caviae, which was isolated from nine cases, was found to be the most predominant isolate, followed by a. veronii biovar sobria, isolated from six cases, and a. hy ... | 1998 | 10211427 |
| antimicrobial activity of essential oils and other plant extracts. | the antimicrobial activity of plant oils and extracts has been recognized for many years. however, few investigations have compared large numbers of oils and extracts using methods that are directly comparable. in the present study, 52 plant oils and extracts were investigated for activity against acinetobacter baumanii, aeromonas veronii biogroup sobria, candida albicans, enterococcus faecalis, escherichia col, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella enterica subsp. enterica s ... | 1999 | 10438227 |
| diverse restriction fragment length polymorphism patterns of the pcr-amplified 16s rrna genes in aeromonas veronii strains and possible misidentification of aeromonas species. | restriction fragment length polymorphism analysis after pcr amplification (rflp-pcr) of the 16s rrna gene has been previously proposed as a rapid method to identify aeromonas species. in the present study, the precision of rflp-pcr was evaluated with 62 aeromonas reference strains. the analysis revealed that aeromonas veronii biovar sobria strains produce various patterns, possibly leading to its misidentification as an environmental species. for most other aeromonas species little variation was ... | 1999 | 10488176 |
| functional characterization of type iv pili expressed on diarrhea-associated isolates of aeromonas species. | our past work has shown that long, flexible type iv pili (single or in bundles) are the predominant pili expressed on fecal isolates of diarrhea-associated species of aeromonas (aeromonas veronii biovar sobria and a. caviae). they represent a family of type iv pili which we have designated bfp (for bundle-forming pili). reports from japan suggest that bfp are intestinal colonization factors. this study presents compelling evidence to support this conclusion. aeromonas bacteria and/or bfp purifie ... | 1999 | 10496928 |
| interaction between aeromonas veronii and epithelial cells of spotted sand bass (paralabrax maculatofasciatus) in culture. | an in vitro fish model to study the interaction between aeromonas veronii and skin, gill and intestinal epithelial cells was developed using primary cultures of mucosal cells (isolated from healthy organisms). primary cultures were exposed to aeromonas veronii strain a186 isolated from a patient with severe gastrointestinal disease. microbial adherence was assessed by a spectrophotometric evaluation of an enzyme-linked, biotin-streptavidin aer. veronii cell-adhesion assay to confluent monolayers ... | 2000 | 10792551 |
| anti-adhesive activity of sulphated exopolysaccharides of microalgae on attachment of red sore disease-associated bacteria and helicobacter pylori to tissue culture cells. | because of the affinity of certain bacterial species for sulphated glycoconjugates exposed on the epithelial cells of susceptible hosts, we hypothesized that sulphated exopolysaccharides of microalgae can be used in anti-adhesive therapies against bacterial infections, both in cold- and warm-blooded animals. in this study we found that adhesion of the human pathogen helicobacter pylori to the hela s3 cell line, and adhesion of the fish pathogens vibrio campbellii, v. ordalii, streptococcus sapro ... | 2000 | 10849279 |
| typing of clinical and environmental aeromonas veronii strains based on the 16s-23s rdna spacers. | genetic relationships of aeromonas veronii strains isolated from human and environmental sources were investigated by restriction fragment length polymorphism (rflp) of the polymerase chain reaction-amplified intergenic spacer region (isr) flanked by the 16s and 23s rrna genes. when using endonucleases alui, hinfi and cfoi the 16s-23s rdna-rflp patterns showed considerable overall similarity, although most strains yielded specific profiles. several intra-specific lines of descent comprised clini ... | 2000 | 10865175 |
| virulence factors in aeromonas veronii bv veronii. | | 1999 | 10897894 |
| spontaneous bacterial empyema caused by aeromonas veronii biotype sobria. | spontaneous bacterial empyema is a complication of hepatic hydrothorax in cirrhotic patients. the pathogen, clinical course and treatment strategy are different to the empyema secondary to pneumonia. a 54-year-old man, who was a cirrhotic patient with hepatic hydrothorax, was admitted to national taiwan university hospital for fever, dyspnea and right side pleuritic pain. the image study revealed massive right pleural effusion and no evidence of pneumonia. the culture of pleural effusion yielded ... | 2000 | 10974579 |
| epidemiological relationships between aeromonas strains isolated from symptomatic children and household environments as determined by ribotyping. | ribotyping was used to study the epidemiology of aeromonas associated gastro-enteritis in young children. ribotyping patterns of 29 aeromonas strains (16 aeromonas caviae, 8 aeromonas hydrophila, 3 aeromonas eucrenophila, 1 aeromonas veronii, and 1 aeromonas encheleia) isolated from primary stool cultures of sick children were compared using the gelcompare software with patterns of 104 strains (39 aeromonas eucrenophila, 29 aeromonas caviae, 11 aeromonas encheleia, 10 aeromonas hydrophila, 6 aer ... | 2000 | 10997832 |
| production of heat-labile enterotoxin by strains of aeromonas veronii bv veronii. | three isolates of aeromonas veronii bv veronii (2 environmental, one blood infection) were examined to see if they produce any enterotoxin and, if so, to determine its characteristics. two isolates caused fluid accumulation in the initial rabbit ileal loop tests. the other strain did so after a single passage through the rabbit ileal loop. all the isolates showed gradual enhancement of fluid secretion after each subsequent passage. inocula of 1 x 10(4) viable cells and 0.25 ml of culture filtrat ... | 2000 | 11014770 |
| identification of genes associated with copper tolerance in an adhesion-defective mutant of aeromonas veronii biovar sobria. | tnphoa mutagenesis was used to identify adhesins of aeromonas veronii biovar sobria 3767, a strain isolated from a diarrhoeal stool specimen. six mutants, from a library of 154, exhibited significantly reduced levels of adhesion to hep-2 cells. primers to the terminal regions of tnphoa were used for inverse pcr and the product from one mutant was cloned into pbluescript and partial sequence data obtained. scanning genbank and embl data bases revealed dna sequence similarity to the copa gene of p ... | 2000 | 11024350 |
| production and secretion of collagen-binding proteins from aeromonas veronii. | collagen-binding protein (cnbp) synthesized by aeromonas veronii is located conserved within the subcellular fraction. the results of this study show that 98% of the total cnbp produced by aer. veronii is present in the extracellular medium, and that the remaining cnbp is distributed either on the cell surface, within the periplasm or anchored on the outer membrane. cnbp is specifically secreted from aer. veronii into the culture medium, because all the beta-lactamase activity was located in the ... | 2000 | 11054164 |
| ingested blood contributes to the specificity of the symbiosis of aeromonas veronii biovar sobria and hirudo medicinalis, the medicinal leech. | hirudo medicinalis, the medicinal leech, usually carries in its digestive tract a pure culture of aeromonas veronii bv. sobria. such specificity is unusual for digestive tracts that are normally colonized by a complex microbial consortium. important questions for the symbiotic interaction and for the medical application after microvascular surgery are whether other bacteria can proliferate or at least persist in the digestive tract of h. medicinalis and what factors contribute to the reported sp ... | 2000 | 11055917 |
| isolation and 16s rrna sequence analysis of the beneficial bacteria from the rhizosphere of rice. | the present study deals with the isolation of plant growth promoting rhizobacteria (pgpr) from rice (variety niab irri-9) and the beneficial effects of these inoculants on two basmati rice varieties. nitrogen-fixing activity (acetylene-reduction activity) was detected in the roots and submerged shoots of field-grown rice variety niab irri-9. estimation of the population size of diazotrophic bacteria by ara-based mpn (acetylene reduction assay-based most probable number) in roots and shoots indic ... | 2001 | 11261489 |
| mucosal immune response of spotted sand bass paralabrax maculatofasciatus (steindachner, 1868) orally immunised with an extracellular lectin of aeromonas veronii. | to assess the immunogenic and immunoprotective role of the extracellular lectin from aeromonas veronii (mcbp), which has affinity for mucosal constituents such as mucin, lactoferrin, immunoglobulins and collagen, spotted sand bass (paralabrax maculatofasciatus) were orally immunised either with soluble mcbp, adjuvant-conjugated mcbp or immobilised mcbp on latex microspheres. the results suggest that the mcbp is capable of eliciting protective immunity against a. veronii infections when administe ... | 2001 | 11308074 |
| mesophilic aeromonads in wild and aquacultured freshwater fish. | numbers and species of motile aeromonas were determined in freshly caught freshwater fish, in the surrounding environment, and also during iced chilled storage of fish specimens. although no significant differences were observed in water samples, initial levels for skin, gill, and intestines were significantly lower in farmed rainbow trout (oncorhynchus mykiss) than in wild brown trout (salmo trutta) and pike (esox lucius). during storage of wild specimens, naturally occurring aeromonads grew fa ... | 2001 | 11348001 |
| new dna-dna hybridization and phenotypic data on the species aeromonas ichthiosmia and aeromonas allosaccharophila: a. ichthiosmia schubert et al. 1990 is a later synonym of a. veronii hickman-brenner et al. 1987. | previously, a dna fingerprinting study based on amplified fragment length polymorphism (aflp) analysis has revealed a possible genotypic resemblance of the species aeromonas ichthiosmia and aeromonas allosaccharophila to aeromonas veronii (huys et al., int. j. syst. bacteriol. 46, 572-580 [19961). currently, two genotypically indistinguishable biovars are known to exist in the latter species, namely a. veronii biovar sobria and a. veronii biovar veronii. in the current study, new dna-dna hybridi ... | 2001 | 11518320 |
| helicobacter pylori and other enteric bacteria in freshwater environments in mexico city. | helicobacter pylori infection is common in the mexican population; however, sources, routes, and risk factors for infection as well as mode of transmission remain unclear. | 2001 | 11578764 |
| three cases of bacteraemia caused by aeromonas veronii biovar sobria. | septicaemia caused by aeromonas species is a life-threatening condition, arising primarily in immunocompromised patients, which has rarely been reported in scandinavia. herein we describe 3 cases of aeromonas sobria bacteraemia from denmark. all the patients were male and all 3 cases occurred during the summer. two patients had acute leukaemia and hiv infection, respectively, while the third patient had colorectal cancer diagnosed several years later. the clinical presentation in all patients wa ... | 2001 | 11669236 |
| identification and characterization of pathogenic aeromonas veronii biovar sobria associated with epizootic ulcerative syndrome in fish in bangladesh. | sparse information is available on the virulence factors of aeromonas strains isolated from diseased fish, from the environment, and from humans. in the present study, 52 aeromonas isolates obtained from epizootic ulcerative syndrome (eus) lesions in fish, from the aquatic environment, and from children with diarrhea in bangladesh were identified by biochemical phenotyping (i.e., pheneplate [php] typing) and dna fingerprinting and then characterized with respect to certain putative virulence fac ... | 2002 | 11823203 |
| microbial populations of an upflow anaerobic sludge blanket reactor treating wastewater from a gelatin industry. | the microbial populations of an upflow anaerobic sludge blanket reactor, used for treating wastewater from the gelatin industry, were studied by microbiological methods and phase-contrast and electron microscopy. microscopy examination of the sludge showed a complex mixture of various rod-shaped and coccoid bacterial pluslong filaments and verymobile curved rods. in addition free-living anaerobic ciliates and flagellates were also observed. the trophic group population observed in decreasing ord ... | 2001 | 11873883 |
| in vitro antimicrobial susceptibility of clinical isolates of aeromonas caviae, aeromonas hydrophila and aeromonas veronii biotype sobria. | | 2002 | 11909850 |
| incidence of toxic aeromonas isolated from food and human infection. | one hundred and ninety four aeromonas isolates (99 from food and 95 from clinical sources) were analyzed as to the species involved and the toxins produced. of the clinical isolates of aeromonas, 29.4% were enterotoxigenic, 43.1% were hemolytic and 89% were cytotoxigenic. among the food isolates, 18.2% were enterotoxigenic, 17.1% were hemolytic and 72.7% were cytotoxigenic. aeromonas sobria and aeromonas veronii produced more enterotoxin and cytotoxin than the other isolates, whereas a. veronii ... | 2002 | 11934569 |
| first documented case of aeromonas veronii biovar sobria infection in northern europe. | the first scandinavian case of aeromonas veronii biovar sobria infection, an episode of peritonitis in a patient suffering from a cirrhotic liver and an earlier unrecognized appendiceal cancer, is described. | 2002 | 12030401 |
| virulence markers in aeromonas hydrophila and aeromonas veronii biovar sobria isolates from freshwater fish and from a diarrhoea case. | to evaluate the public health significance of representative strains of two aeromonas spp., mainly from freshwater fish, on the basis of production of virulence-associated factors and presence of the haemolytic genes aera and hlya. | 2002 | 12174039 |
| aeromonas culicicola sp. nov., from the midgut of culex quinquefasciatus. | the taxonomic position was examined of three isolates, mtcc 3249t, sh and slh, from the midgut of female culex quinquefasciatus and aedes aegyptii mosquitoes. numbers of cells of these isolates increased 2000-fold after a blood meal of the mosquitoes. 16s rrna gene sequence analysis of the novel strains showed that they were highly homologous to strains of aeromonas. dna-dna hybridization studies showed that dna of strain mtcc 3249t was 96 and 88% similar to that of strains sh and slh, respectiv ... | 2002 | 12361279 |
| study of the effect of the midgut bacterial flora of culex quinquefasciatus on the susceptibility of mosquitoes to japanese encephalitis virus. | culex quinquefasciatus mosquitoes were collected from the field and microbial flora was isolated from their midgut. isolates belonging to 13 different genera were obtained. four most abundant isolates viz. pseudomonas sp., acinetobacterjunii, staphylococcus epidermis and aeromonas culicicola were used to study their effect on the susceptibility of mosquitoes to japanese encephalitis virus (jev). incorporation of individual isolates in the mosquito bloodmeal resulted in an increased susceptibilit ... | 2002 | 12693864 |
| molecular cloning, sequencing and characterization of omp48, the gene encoding for an antigenic outer membrane protein from aeromonas veronii. | to clone, sequence and characterize the gene encoding the omp48, a major outer membrane protein from aeromonas veronii. | 2003 | 12694457 |
| genetic linkage of the penicillinase gene, amp, and blrab, encoding the regulator of beta-lactamase expression in aeromonas spp. | aeromonas hydrophila t429125, a human clinical isolate, possesses three coordinately inducible beta-lactamases encoded by amph (class d beta-lactamase), ceph (class c beta-lactamase) and imih (class b beta-lactamase). we report that upstream of amph there are two genes, blra and blrb, encoding a putative two-component regulatory system. pcr studies revealed the same blrab-amp gene arrangement in all aeromonas spp. isolates tested; namely, aeromonas veronii bv. sobria, aeromonas jandaei, aeromona ... | 2003 | 12746371 |
| characterisation of aeromonas spp. isolated from frozen fish intended for human consumption in mexico. | a total of 82 strains of presumptive aeromonas spp. were identified biochemically and genetically (16s rdna-rflp). the strains were isolated from 250 samples of frozen fish (tilapia, oreochromis niloticus niloticus) purchased in local markets in mexico city. in the present study, we detected the presence of several genes encoding for putative virulence factors and phenotypic activities that may play an important role in bacterial infection. in addition, we studied the antimicrobial patterns of t ... | 2003 | 12781953 |
| complement resistance is essential for colonization of the digestive tract of hirudo medicinalis by aeromonas strains. | from the crop of the medicinal leech, hirudo medicinalis, only aeromonas veronii bv. sobria can be cultured consistently. serum-sensitive a. veronii mutants were unable to colonize h. medicinalis, indicating the importance of the mammalian complement system for this unusual simplicity. complementation of one selected mutant restored its ability to colonize. serum-sensitive mutants are the first mutant class with a colonization defect for this symbiosis. | 2003 | 12839811 |
| phylogenetic affiliation of aeromonas culicicola mtcc 3249(t) based on gyrb gene sequence and pcr-amplicon sequence analysis of cytolytic enterotoxin gene. | we determined the gyrb gene sequences of all 17 hybridizations groups of aeromonas. phylogenetic trees showing the evolutionary relatedness of gyrb and 16s rrna genes in the type strains of aeromonas were compared. using this approach, we determined the phylogenetic position of aeromonas culicicola mtcc 3249(t), isolated from midgut of culex quinquefasciatus. in the gyrb based-analysis a. culicicola mtcc 3249(t) grouped with a. veronii whereas, it grouped with a. jandaei in the 16s rrna based tr ... | 2003 | 12866846 |
| aeromonas veronii biovar veronii septicaemia and acute suppurative cholangitis in a patient with hepatitis b. | gram-negative bacilli of the genus aeromonas are widespread in aquatic environments and can be responsible for human infections. although aeromonas extraintestinal and systemic infections have been reported with growing frequency in recent years, aeromonas septicaemia remains an uncommon finding, often associated with serious underlying disease and predominantly related to the species aeromonas hydrophila, aeromonas veronii biovar sobria and aeromonas caviae. here, a case of a. veronii biovar ve ... | 2003 | 12867570 |
| misidentification of aeromonas veronii biovar sobria as vibrio alginolyticus by the vitek system. | to find the cause of misidentification of aeromonads when using the vitek system. | 2003 | 12969502 |
| taxonomic study of sucrose-positive aeromonas jandaei-like isolates from faeces, water and eels: emendation of a. jandaei carnahan et al. 1992. | fourteen sucrose-positive aeromonas jandaei-like isolates from fresh water and reared european eels were subjected to a polyphasic study to determine their taxonomic position. numerical taxonomy was used to analyse phenotypic data obtained for these isolates and 43 type and reference strains representative of recognized aeromonas species. the a. jandaei cluster (phenon 1) was defined at 81.6 % similarity (s(j)); this included the a. jandaei-like isolates, the sucrose-positive strain aeromonas ve ... | 2003 | 13130026 |
| analysis of 16s-23s intergenic spacer regions and rrn operon copy number of aeromonas culicicola mtcc 3249t. | the 16s-23s intergenic spacer and 23s rrna gene sequence were determined for a. culicicola mtcc 3249t. ten different isr, indicative of ten rrn operons, were found in the strain that were grouped in three major types. of the three types, isr i was non-coding while isr ii and iii coded for trna(glu)(uuc). the trna(glu)(uuc) sequence was identical to that of e. coli. both isr i and ii were represented by three clones whereas four clones represented isr iii. the number of nucleotide differences bet ... | 2003 | 14509830 |
| molecular characterization and distribution of virulence-associated genes amongst aeromonas isolates from libya. | the aim of the study was to type 52 aeromonas spp. isolates from chicken carcasses, children with diarrhoea and a hospital environment in libya, and to determine the distribution of putative virulence genes amongst them. | 2003 | 14633028 |
| [a successful treatment of progressive necrotizing myositis caused by aeromonas veronii biotype sobria]. | we report a 52 year-old patient with malignant lymphoma who developed necrotizing soft tissue infection in the left lower extremity by aeromonas veronii biotype sobria during a neutropenic period after intensive chemotherapy. we decided to amputate the involved leg when signs and symptoms of necrotizing myositis were progressing despite administration of sensitive antibiotics. he survived with subsequent intensive supportive care. it is to note that even though the patient's neutrophil count is ... | 2003 | 14672014 |
| hemolytic and proteolytic activities of aeromonas hydrophila and aeromonas veronii biovar sobria in broth and salmon extract at different temperatures. | expression of hemolytic and proteolytic activities throughout the growth cycle was investigated with two enterotoxic aeromonad strains assigned to the species aeromonas hydrophila and aeromonas veronii biovar sobria. although growth kinetic data were dependent on strain, temperature, and substrate, maximum populations attained were higher than 9 log cfu/ml in aerated tryptone soya broth plus yeast extract (tsbye) and salmon extract within the range 4 to 28 degrees c. for both strains in tsbye, v ... | 2004 | 14968959 |
| an aeromonas veronii biovar sobria infection with disseminated intravascular gas production. | we report a case of aeromonas veronii biovar sobria infection with disseminated intravascular gas production. the patient was an afebrile 15-year-old girl who had been quite healthy until the onset of the illness. she came to the hospital because of a 6-h history of increasing pain and swelling in her left thigh. on admission, no infection was suspected, and a tentative diagnosis of a ruptured left gracilis muscle was made. because the pain increased continuously, the treatment concentrated on p ... | 2004 | 14991516 |
| type iii secretion system genes in clinical aeromonas isolates. | we have identified the genes ascf and ascg, which encode components of a putative type iii secretion system (ttss) in aeromonas: we investigated the distribution of these and other ttss genes in 84 clinical isolates and found hybridizing sequences in 50% of the strains, with a higher prevalence in aeromonas hydrophila and aeromonas veronii than in aeromonas caviae. | 2004 | 15004096 |
| adhesive properties of a lamb-like outer-membrane protein and its contribution to aeromonas veronii adhesion. | to identify and characterize nonfimbrial proteins from aeromonas veronii involved in the attachment to epithelial cells in vitro. | 2004 | 15012808 |
| aerolysin is activated by metalloprotease in aeromonas veronii biovar sobria. | aeromonas veronii is an opportunistic human pathogen that causes diarrhoea and extraintestinal infections, such as wound infection and septicaemia. an a. veronii protease (avp) from a biovar sobria strain, aeg1, was partially purified and characterized. mature avp hydrolysed casein but not elastin, and protease activity was inhibited by metalloprotease inhibitors. nucleotide sequence analysis showed that avp belongs to the thermolysin family of proteases. an avp-deficient mutant was constructed ... | 2004 | 15150325 |
| prevalence, serotype distribution, antibiotic susceptibility and genetic profiles of mesophilic aeromonas species isolated from hospitalized diarrhoeal cases in kolkata, india. | a comprehensive study was performed to examine incidence, species distribution, drugs sensitivity, virulence genes and molecular fingerprints of aeromonas species isolated from patients with acute diarrhoea over a period of 2 years in kolkata, india. following the aerokey ii scheme, more than 95 % of strains were identified to species level. seven different species were encountered in this study, with aeromonas caviae being dominant, followed by aeromonas hydrophila and aeromonas veronii biovar ... | 2004 | 15150333 |
| the usefulness of molecular techniques to assess the presence of aeromonas spp. harboring virulence markers in foods. | a total of 78 raw and 123 processed and ready-to-eat retail food samples were used to assess the presence of motile aeromonas spp. harboring virulence genes (cytotoxic enterotoxin and hemolysin genes) using a recently described pcr method in comparison with the conventional cultivation method based on the use of ampicillin-dextrin agar (ada) medium. with the ada-based method, 65/201 (32.3%) samples showed presumptive aeromonas spp. colonies whereas the pcr method revealed the presence of aeromon ... | 2004 | 15193799 |
| studies on cultured and uncultured microbiota of wild culex quinquefasciatus mosquito midgut based on 16s ribosomal rna gene analysis. | to describe the midgut microbial diversity and the candidate bacteria for the genetic manipulation for the generation of transgenic mosquitoes refractory to transmission of diseases, the microbiota of wild culex quinquefasciatus mosquito midgut was studied using a conventional culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. the culturable microbiota was identified as acinetobacter junii, ac. calcoaceticus, aeromonas culicicola, bacillus thuringiensis, microbac ... | 2004 | 15210998 |
| over-expression, purification, and characterization of metallo-beta-lactamase imis from aeromonas veronii bv. sobria. | the gene from aeromonas veronii bv. sobria encoding the metallo-beta-lactamase imis was subcloned into pet-26b, and imis was over-expressed in bl21(de3) escherichia coli and purified using sp-sepharose chromatography. this protocol yielded over 5 mg of imis per liter of growth culture under optimum conditions. the biochemical properties of recombinant imis were compared with those of native imis. recombinant and native imis have the same n-terminus of a-g-m-s-l, and cd spectroscopy was used to s ... | 2004 | 15249050 |
| phylogenetic analysis of the genus aeromonas based on two housekeeping genes. | the phylogenetic relationships of all known species of the genus aeromonas, and especially aeromonas bestiarum and aeromonas salmonicida, were investigated on 70 strains using the rpod sequence, which encodes the sigma70 factor. this analysis was complemented with the sequence of gyrb, which has already proven useful for determining the phylogenetic relationships in the genus. nucleotide sequences of rpod and gyrb showed that both genes had similar substitution rates (< 2 %) and a similar number ... | 2004 | 15388703 |
| bulgecin a: a novel inhibitor of binuclear metallo-beta-lactamases. | bulgecin a, a sulphonated n-acetyl-d-glucosamine unit linked to a 4-hydroxy-5-hydroxymethylproline ring by a beta-glycosidic linkage, is a novel type of inhibitor for binuclear metallo-beta-lactamases. using steady-state kinetic analysis with nitrocefin as the beta-lactam substrate, bulgecin a competitively inhibited the metallo-beta-lactamase bceii from bacillus cereus in its two-zinc form, but failed to inhibit when the enzyme was in the single-zinc form. the competitive inhibition was restore ... | 2005 | 15569001 |
| first record of the rare species aeromonas culicicola from a drinking water supply. | we describe the recovery of the rare species aeromonas culicicola, so far known only in mosquitoes in india, from a drinking water supply in spain. typing, using enterobacterial repetitive intergenic consensus-pcr, revealed that the 27 new isolates belonged to 3 very closely related strains. these strains were genetically identified by 16s rrna gene sequencing. spanish strains differed from the mosquito strains in three nucleotide positions. the ahcytoen gene was present in these water strains, ... | 2005 | 15640231 |
| freshwater selenium-methylating bacterial thiopurine methyltransferases: diversity and molecular phylogeny. | the diversity of bacterial thiopurine methyltransferases (btpmt) among five natural se-methylating freshwaters was investigated by polymerase chain reaction (pcr) screenings and sequencings. dna sequence analyses confirmed the cloned products' identity and revealed a broad diversity of freshwater tpmts. neighbour-joining (nj) phylogenetic analyses combining these sequences, all genbank entries closely related to these sequences and deduced tpmts obtained in this work from selected gamma-proteoba ... | 2005 | 15658983 |
| evaluation of dna vaccination of spotted sand bass (paralabrax maculatofasciatus) with two major outer-membrane protein-encoding genes from aeromonas veronii. | genes encoding two major outer membrane proteins (omps) of the bacterial pathogen aeromonas veronii, omp38 and omp48, were used to construct dna vaccines. the protective effect of such vaccines against motile aeromonad septicaemia was evaluated in spotted sand bass (paralabrax maculatofasciatus), an endemic species of the mexican northwest pacific coast and a potential resource for the aquaculture industry. weak protein expression, as determined by immunoblotting, was observed after transfection ... | 2005 | 15752654 |
| spectroscopic studies on cobalt(ii)-substituted metallo-beta-lactamase imis from aeromonas veronii bv. sobria. | in an effort to probe the structure of a group bb metallo-beta-lactamase, co(ii)-substituted imis was prepared and characterized by electronic absorption, nmr, and epr spectroscopies. imis containing 1 equiv of co(ii) (co(ii)(1)-imis) was shown to be catalytically active. electronic absorption studies of co(ii)(1)-imis revealed the presence of two distinct features: (1) an intense sulfur to co(ii) ligand to metal charge transfer band and (2) less intense, co(ii) ligand field transitions that sug ... | 2005 | 15794654 |
| new gammaproteobacteria associated with blood-feeding leeches and a broad phylogenetic analysis of leech endosymbionts. | many monophagous animals have coevolutionary relationships with bacteria that provide unavailable nutrients to the host. frequently, these microbial partners are vertically inherited and reside in specialized structures or tissues. here we report three new lineages of bacterial symbionts of blood-feeding leeches, one from the giant amazonian leech, haementeria ghilianii, and two others from placobdelloides species. these hosts each possess a different mycetome or esophageal organ morphology wher ... | 2005 | 16151107 |
| aeromonas culicicola pidiyar et al. 2002 is a later subjective synonym of aeromonas veronii hickman-brenner et al. 1987. | we re-investigated the taxonomic affiliation between aeromonas culicicola pidiyar et al. 2002 and aeromonas veronii hickman brenner et al. 1987, two species that have previously been shown to be closely related on the basis of gyrb and rpod gene sequencing and amplified fragment length polymorphism fingerprinting. from extended biochemical characterization and fatty acid analysis, it was concluded that a. culicicola did not constitute a unique phenotypic group in the genus aeromonas but instead ... | 2005 | 16156118 |
| evolutionary and diagnostic implications of intragenomic heterogeneity in the 16s rrna gene in aeromonas strains. | sequencing 16s rrna genes (ssu) cloned from aeromonas strains revealed that strains contained up to six copies differing by < or = 1.5%. the ssu copies from aeromonas veronii lmg13695 clustered with sequences from four aeromonas species. these results demonstrate intragenomic heterogeneity of ssu and suggest caution when using ssu to identify aeromonads. | 2005 | 16159790 |
| the major aeromonas veronii outer membrane protein: gene cloning and sequence analysis. | the gene encoding the major outer membrane protein (omp) from aeromonas veronii, omp38, was cloned and characterized. sequence analysis revealed an open reading frame of 1,047 nucleotides coding for a primary protein of 349 amino acids with a 20-amino-acid signal peptide at the n-terminal and the consensus sequence ala-x-ala (ala-asn-ala) as the signal peptidase i recognition site. the mature protein is composed of 329 amino acids with a calculated molecular mass of 36,327 da. the degree of iden ... | 2005 | 16252131 |