| [the thiobarbituric method for analyzing sugars. iii. a method for studying sorbitol dehydrogenase activity]. | a method for studying of sorbitol dehydrogenase activity of acetobacter suboxydans has been worked out which can be applied to other microbe cells too. the enzyme transformation of d-sorbitol into l-sorbose is determined through the reaction between ketohexose and 2-thiobarbituric acid under heating in 1 m oxalic acid (ph = 0.64) medium for 15 or 30 min. the yellow-coloured product was measured at 400 nm. the method is characterized with its high specificity, good reproducibility and accuracy, w ... | 1990 | 2382593 |
| isolation of a new restriction enzyme, apaci, an isoschizomer of bamhi produced by acetobacter pasteurianus. | a new type ii restriction endonuclease apaci purified from acetobacter pasteurianus is an isoschizomer of bamhi that cleaves at the nucleotide sequence 5'-g/gatcc-3' of double-stranded dna. the single restriction activity present in this strain permits rapidly purified 30,000 units of cleavage activity from 10 g of freshly harvested cells. the resulting apaci preparation is free of contaminant nuclease activities that might interfere with in vitro manipulation of dna. | 1992 | 1493903 |
| apaci, an isoschizomer of bamhi isolated from acetobacter pasteurianus. | | 1992 | 1630925 |
| gene expression in cotton (gossypium hirsutum l.) fiber: cloning of the mrnas. | cotton, an important natural fiber, is a differentiated epidermal cell. the number of genes that are active in fiber cells is similar to those in leaf, ovule, or root tissues. through differential screening of a fiber cdna library, we isolated five cdna clones that are preferentially expressed in fiber. one of the cdna clones, pcke6, corresponded to an abundant mrna in fiber. transcripts for e6 were detected throughout the development of the fiber. immunoprecipitation of in vitro translation pro ... | 1992 | 1631059 |
| cloning of a gene involved in cellulose biosynthesis in acetobacter xylinum: complementation of cellulose-negative mutants by the udpg pyrophosphorylase structural gene. | three cellulose-negative (cel-) mutants of acetobacter xylinum strain atcc 23768 were complemented by a cloned 2.8 kb dna fragment from the wild type. biochemical analysis of the mutants showed that they were deficient in the enzyme uridine 5'-diphosphoglucose (udpg) pyrophosphorylase. the analysis also showed that the mutants could synthesize beta(1-4)-glucan in vitro from udpg, but not in vivo from glucose. this result was expected, since udpg is known to be the precursor for cellulose synthes ... | 1989 | 2549367 |
| cellulose saccharification for fermentation industry. applications. | | 1976 | 1000071 |
| effects of the yeast extract components pyrroloquinoline quinone and aspartic acid on vitamin b12 production in klebsiella pneumoniae ifo 13541. | the production of vitamin b12 from carbohydrates, peptone, casamino acid, etc., by intestinal bacteria was investigated. klebsiella pneumoniae ifo 13541 was the most efficient strain for vitamin b12 production, which depended exclusively on the concentration of yeast extract added to the medium. a concentrated solution of yeast extract (1 ml) was chromatographed on a sephadex g-25 column (1 x 180 cm) and eluted with h2o (eighty fractions of 3 ml each were collected). it was found that fractions ... | 1989 | 2561367 |
| cloning and sequencing of the cellulose synthase catalytic subunit gene of acetobacter xylinum. | the gene for the catalytic subunit of cellulose synthase from acetobacter xylinum has been cloned by using an oligonucleotide probe designed from the n-terminal amino acid sequence of the catalytic subunit (an 83 kda polypeptide) of the cellulose synthase purified from trypsin-treated membranes of a. xylinum. the gene was located on a 9.5 kb hind iii fragment of a. xylinum dna that was cloned in the plasmid puc18. dna sequencing of approximately 3 kb of the hind iii fragment led to the identific ... | 1990 | 2151718 |
| cloning of genes responsible for acetic acid resistance in acetobacter aceti. | five acetic acid-sensitive mutants of acetobacter aceti subsp. aceti no. 1023 were isolated by mutagenesis with n-methyl-n'-nitro-n-nitrosoguanidine. three recombinant plasmids that complemented the mutations were isolated from a gene bank of the chromosome dna of the parental strain constructed in escherichia coli by using cosmid vector pmvc1. one of these plasmids (par1611), carrying about a 30-kilobase-pair (kb) fragment that conferred acetic acid resistance to all five mutants, was further a ... | 1990 | 2156811 |
| atomic-resolution structure of the cellulose synthase regulator cyclic diguanylic acid. | cyclic diguanylic acid acts as a regulator for cellulose synthase activity in the bacterium acetobacter xylinum. we report the x-ray crystal structure of the regulator at atomic resolution. the structure contains two independent molecules that adopt almost identical conformations. the two molecules form self-intercalated units that are stacked on each other. two different g.g base-pairing modes occur between the stacks. the more stable one has two or possibly three hydrogen bonds between two gua ... | 1990 | 2158107 |
| cloning and sequencing of the gene encoding the 72-kilodalton dehydrogenase subunit of alcohol dehydrogenase from acetobacter aceti. | a genomic library of acetobacter aceti dna was constructed by using a broad-host-range cosmid vector. complementation of a spontaneous alcohol dehydrogenase-deficient mutant resulted in the isolation of a plasmid designated paa701. subcloning and deletion analysis of paa701 limited the region that complemented the deficiency in alcohol dehydrogenase activity of the mutant. the nucleotide sequence of this region was determined and showed that this region contained the full structural gene for the ... | 1989 | 2722742 |
| mutagenesis of acetobacter methanolicus mb58 with the transposon tn5. | transposon mutagenesis was applied to the isolation of mutants of the facultatively methylotrophic acetobacter methanolicus mb 58. the transposon tn5 (psu2011) was transferred from escherichia coli sm 10 by means of conjugation to acetobacter methanolicus mb 58. four out of 1850 stable km-resistant transconjugants were identified that were formaldehyde sensitive and failed to grow on methanol. | 1990 | 2166787 |
| the cyclic diguanylic acid regulatory system of cellulose synthesis in acetobacter xylinum. chemical synthesis and biological activity of cyclic nucleotide dimer, trimer, and phosphothioate derivatives. | an unusual compound, cyclic-bis(3'----5') diguanylic acid (c-di-gmp or cgpgp), is involved in the regulation of cellulose synthesis in the bacterium acetobacter xylinum. this cyclic dinucleotide acts as an allosteric, positive effector of cellulose synthase activity in vitro (ka = 0.31 microm) and is inactivated via degradation by a ca2(+)-sensitive phosphodiesterase, pde-a (km = 0.25 microm). a series of 13 analogs cyclic dimer and trimer nucleotides were synthesized, employing a phosphotrieste ... | 1990 | 2172238 |
| phthoxazolin, a specific inhibitor of cellulose biosynthesis, produced by a strain of streptomyces sp. | | 1990 | 2211353 |
| cytochrome a1 of acetobacter aceti is a cytochrome ba functioning as ubiquinol oxidase. | cytochrome a1 is a classic cytochrome that in the 1930s had already been detected in acetobacter strains and in the 1950s was identified as a terminal oxidase. however, recent studies did not substantiate the previous observations. we have detected a cytochrome a1-like chromophore in acetobacter aceti, which was purified and characterized in this study. the cytochrome was solubilized from membranes of the strain with octyl beta-d-glucopyranoside and was purified by single column chromatography. ... | 1990 | 2263637 |
| 3-hexulose-6-phosphate synthase from acetobacter methanolicus mb58. | | 1990 | 2280713 |
| acetobacter cellulose pellicle as a temporary skin substitute. | a bacterial strain with morphological and biochemical properties close to acetobacter xylinum has been cultured in nonagitated, inverted sucrose- and yeast water-based medium for the production of thick, smooth, and floating cellulosic pellicles. the cellulose content (greater than 90%, dry weight, depending on the efficiency of water washing) and the beta-d-homopolyglucan nature of these pellicles were assessed by physical, chemical, and enzymatic methods. the apyrogenic bacterial biomass, a mi ... | 1990 | 2353811 |
| selective and rapid solubilization of the microbial membrane enzyme aldehyde dehydrogenase. | an improved solubilization procedure for the membrane-bound quinoprotein aldehyde dehydrogenase from acetobacter rancens ccm 1774 was established. after the first solubilization of membrane enzymes by brij 35 which provided important extraction of membrane proteins other than aldehyde dehydrogenase, the application of trition x-100 resulted in an almost 20-fold purification of quinoprotein aldehyde dehydrogenase. the optimal solubilization was closely connected with definite detergent/protein ra ... | 1990 | 2384875 |
| molecular origins of acetan solution properties. | acetan is a branched acidic heteropolysaccharide secreted by acetobacter xylinum. x-ray diffraction studies of oriented fibres suggest non-crystalline helices with fivefold symmetry and a pitch of 4.8 nm. optical rotation and circular dichroism studies are consistent with the retention of the helical structure in solution and a helix-coil transition upon heating and cooling. aqueous solutions yield high 'low shear rate' viscosity and shear-thin upon shearing. | 1989 | 2489099 |
| a fast spheroplast formation procedure in some 2,5-diketo-d-gluconate- and 2-keto-l-gulonate- producing bacteria. | calcium 2-keto-l-gulonate (ca-2-klg, a key intermediate in vitamin c synthesis) is produced from calcium 2,5-diketo d-gluconate (ca-2,5-dkg) by a variety of bacteria. a few bacterial species which efficiently convert glucose to ca-2,5-dkg have been isolated in our laboratory. our bacterial collection included species that possess the genes for production of ca-2-klg from ca-2,5-dkg; however, the yield of the former is poor. a procedure for the preparation of spheroplasts in ca-2,5-dkg- and ca-2- ... | 1989 | 2517394 |
| change of the terminal oxidase from cytochrome a1 in shaking cultures to cytochrome o in static cultures of acetobacter aceti. | acetobacter aceti has an ability to grow under two different culture conditions, on shaking submerged cultures and on static pellicle-forming cultures. the respiratory chains of a. aceti grown on shaking and static cultures were compared, especially with respect to the terminal oxidase. little difference was detected in several oxidase activities and in cytochrome b and c contents between the respiratory chains of both types of cells. furthermore, the results obtained here suggested that the res ... | 1992 | 1729204 |
| metabolism of the 18o-methoxy substituent of 3-methoxybenzoic acid and other unlabeled methoxybenzoic acids by anaerobic bacteria. | o-methyl substituents of aromatic compounds can provide c1 growth substrates for facultative and strict anaerobic bacteria isolated from diverse environments. the mechanism of the bioconversion of methoxylated benzoic acids to the hydroxylated derivatives was investigated with a model substrate and cultures of one anaerobic consortium, eight strict anaerobic bacteria, and one facultative anaerobic microorganism. using high-pressure liquid chromatography and gas chromatography-mass spectral analy ... | 1988 | 3389815 |
| identification of a new gene in an operon for cellulose biosynthesis in acetobacter xylinum. | dna sequencing of the region downstream of the cellulose synthase catalytic subunit gene of acetobacter xylinum led to the identification of an open reading frame coding for a polypeptide of 86 kda. the deduced amino acid sequence of this polypeptide matches from position 27 to 40 with the n-terminal amino acid sequence determined for a 93 kda polypeptide that copurifies with the cellulose synthase catalytic subunit during purification of cellulose synthase. the cellulose synthase catalytic subu ... | 1991 | 1830823 |
| direct demonstration of enol-oxaloacetate as an immediate product of malate oxidation by the mammalian succinate dehydrogenase. | rapid malonate-sensitive transitory formation of enol-oxaloacetate followed by slow ketonization of the product was observed after addition of malate to the mammalian succinate-ubiquinone reductase in the presence of electron acceptor. the initial rate of enol-oxaloacetate production was equal to that of malate oxidation. oxaloacetate keto-enol tautomerase had no effect on the initial rate of enol-oxaloacetate production nor on the kinetics of malate oxidation; the enzyme drastically accelerated ... | 1991 | 1864383 |
| [subordination of the taxa of gram-negative bacteria determined by numerical analysis methods]. | various numerical methods were used to estimate the coordination of taxa of gram-negative aerobic and facultative anaerobic organoheterotrophic and chemolithotrophic bacteria. stable phena were found to be formed by cultures belonging to the families rhizobiaceae, halobacteriaceae, enterobacteriaceae, nitrobacteriaceae (except the genus nitrobacter), and methylomonadaceae (except the genus methylococcus). the unstable position was found in the genera thermus, zoogloea, xanthomonas, sulfolobus, m ... | 1986 | 3523170 |
| nature of plant stimulators in the production of acetobacter xylinum ("tea fungus") biofilm used in skin therapy. | caffeine and related xanthines were identified as potent stimulators for the bacterial cellulose production in a. xylinum. these compounds are present in several plants whose infusions are useful as culture-medium supplements for this acetobacterium. the proposed target for these native purine-like inhibitory substances is the novel diguanyl nucleotide phosphodiesterase(s) that participate(s) in the bacterial cellulogenic complex. a better understanding of this feature of a. xylinum physiology m ... | 1991 | 1929372 |
| [acetobacter methanolicus--a new organism for genetic studies]. | a new bacterial strain is described belonging to acetobacter methanolicus species. it is of industrial value as a producer of protein and methanol products. the strain is acidophile and this feature comprises a conspicuous technological advantage. the results of bacteriophage and cell interactions are reported. they might be potentially useful for elaboration of the transduction technique for the strain. the collection of mutants was obtained including those utilizing methanol, having auxotrophi ... | 1985 | 3842739 |
| purification and properties of nadp-linked glucose-6-phosphate dehydrogenase from acetobacter hansenii (acetobacter xylinum). | the nadp-linked glucose-6-phosphate dehydrogenase from acetobacter hansenii (formerly known as acetobacter xylinum) has been purified to apparent homogeneity. the sequence of the 10 n-terminal amino acids was determined. the subunit molecular weight of the enzyme is 53,000 as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis; gel filtration studies under nondenaturing conditions revealed that the molecular weight of the enzyme is 200,000 to 220,000 at ph 6.5 and 9.5, sugges ... | 1991 | 1929428 |
| nucleotide sequence and expression analysis of the acetobacter xylinum uridine diphosphoglucose pyrophosphorylase gene. | the nucleotide sequence of the acetobacter xylinum uridine diphosphoglucose pyrophosphorylase gene was determined; this is the first procaryotic uridine diphosphoglucose pyrophosphorylase gene sequence reported. the sequence data indicated that the gene product consists of 284 amino acids. this finding was consistent with the results obtained by expression analysis in vivo and in vitro in escherichia coli. | 1991 | 1938907 |
| cloning and sequencing of the gene cluster encoding two subunits of membrane-bound alcohol dehydrogenase from acetobacter polyoxogenes. | the membrane-bound alcohol dehydrogenase (adh) from acetobacter polyoxogenes nbi1028 is composed of a 72 kda subunit and a 44 kda cytochrome c subunit. the amino acid sequences of the two regions of the 72 kda subunit were determined to prepare oligonucleotides for the purpose of amplification of a dna fragment corresponding to the intermediate region by the polymerase chain reaction. a 0.5 kb dna fragment thus amplified was used as the probe to clone a 7.0 kb psti fragment coding for the whole ... | 1991 | 2001402 |
| adhesion of 1-carbon utilizing bacteria to polymeric surfaces. | | 1991 | 2037198 |
| biogenesis of bacterial cellulose. | cellulose is the most abundant biological polymer on earth. it is found in wood and cotton, and forms the basic structural foundation of the cell wall of almost all eukaryotic plants. bacteria are known to secrete cellulose as part of their metabolism of glucose and other sugars. the focus of this review is upon bacterial cellulose synthesis. we emphasize recent literature directed primarily upon acetobacter xylinum, which has been most widely studied. our review covers the following topics rela ... | 1991 | 2039586 |
| biooxidation of a synthetic waste by a microbial film grown on the liquid surface in a shallow flow reactor. | a new process of biological waste treatment was developed by use of microbial films grown on the liquid surface in a shallow flow reactor. the performance of this process was tested using a synthetic waste that contained acetic acid as a model organic pollutant. about 90% of acetic acid (10,000 mg/l-1) in the synthetic waste was removed by setting alpha tau: (alpha specific liquid surface area, cm-1, and tau: hydraulic liquid detention time, h) higher than 15 cm-1/h. it was necessary to maintain ... | 1990 | 2091526 |
| identification of the uridine 5'-diphosphoglucose (udp-glc) binding subunit of cellulose synthase in acetobacter xylinum using the photoaffinity probe 5-azido-udp-glc. | photoaffinity labeling of purified cellulose synthase with [beta-32p]5-azidouridine 5'-diphosphoglucose (udp-glc) has been used to identify the udp-glc binding subunit of the cellulose synthase from acetobacter xylinum strain atcc 53582. the results showed exclusive labeling of an 83-kda polypeptide. photoinsertion of [beta-32p]5-azido-udp-glc is stimulated by the cellulose synthase activator, bis-(3'----5') cyclic diguanylic acid. addition of increasing amounts of udp-glc prevents photolabeling ... | 1990 | 2138620 |
| nicotinamide adenine dinucleotide- and nicotinamide adenine dinucleotide phosphate-specific glucose 6-phosphate dehydrogenases of acetobacter xylinum and their role in the regulation of the pentose cycle. | | 1973 | 4144393 |
| the glucose dehydrogenase activity of the nad-linked glucose-6-phosphate dehydrogenase from acetobacter xylinum. | | 1973 | 4148195 |
| [desoxyribonucleic acid hybrids and the bacterial species concept]. | | 1964 | 4157725 |
| lipopolysaccharide-protein interactions: determination of dissociation constants by affinity electrophoresis. | an affinity electrophoresis system is described to allow determination of dissociation constants of lipopolysaccharide (lps)-protein complexes. the lps ligand is incorporated into polyacrylamide gels by addition to the polyacrylamide-n,n'-methylenebisacrylamide polymerization mixture. quantitative evaluation revealed formation of immobile protein-ligand complexes. the method was applied both to r- and s-form lps from acinetobacter calcoaceticus. for a heat-modifiable outer membrane protein with ... | 1989 | 2612487 |
| [effect of actinomycin d and 6-mercaptopurine on the metabolism of nucleic acids in the spleen of the rat after antigenic stimulation]. | | 1969 | 4189844 |
| the microbiology of burukutu beer. | | 1973 | 4204376 |
| comparison of porphyrin and heme biosynthesis in various heterotrophic bacteria. | | 1973 | 4208581 |
| studies on the activated sludge bacteria participating in the biodegradation of methanol, formaldehyde and ethylene glycol. i. isolation and identification. | | 1974 | 4209888 |
| studies on the activated sludge bacteria participating in the biodegradation of methanol, formaldehyde and ethylene glycol. ii. utilization of various carbon and nitrogen compounds. | | 1974 | 4209889 |
| the microbial ecology of tape ketan fermentation. | the growth of fungi, yeasts and bacteria was followed during the fermentation of tape ketan. the tape was prepared using samples of indonesian ragi as inoculum. fermentation was characterized by the dominant growth of amylomyces rouxii and candida pelliculosa (10(5)-10(7) cfu/g), and a lesser contribution from saccharomyces cerevisiae. hansenula anomala grew to a limited extent during some fermentations. bacteria of the genera bacillus and acetobacter contributed also to the fermentation, produc ... | 1989 | 2641491 |
| transformation of organic compounds by microbial enzymes. | | 1974 | 4214719 |
| the dissimilation of glucose and gluconate by acetobacter xylinum. 1. the origin and the fate of triose phosphate. | | 1964 | 4220768 |
| [repression, by fructose, of the biosynthesis of 6-phosphofructokinase and phosphoglyceromutase in acetobacter xylinum]. | | 1969 | 4240519 |
| detoxification of formaldehyde by acetic acid bacteria. | formaldehyde resistance of methylotrophic and non-methylotrophic acetobacter strains was investigated. a facultatively methylotrophic acetobacter methanolicus (mb58) gets rid of free formaldehyde by assimilating it. heterotrophically growing cells tolerate 12 mm free formaldehyde. non-methylotrophic but methanol oxidizing acetobacter pasteurianus strains possess the same level of formaldehyde resistance. formaldehyde resistance can be drastically lowered down to 4 mm by blocking the formate dehy ... | 1989 | 2775425 |
| cellulose biogenesis in bacteria and higher plants is disrupted by magnetic fields. | | 1989 | 2779667 |
| nad+-linked d-mannitol dehydrogenase in acetobacter suboxydans. | | 1966 | 4288131 |
| a biochemical chimera suggesting the existence of at least two dolichol-p-glucose:dolichol-p-p-oligosaccharide glucosyltransferases. | as previously reported, incubation of liver dolichol-p, udp-[14c]gal, and a particulate preparation of acetobacter xylinum leads to the synthesis of dolichol-p-[14c]gal (p. romero, r. garcia, and m. dankert (1977) mol. cell. biochem. 16, 205-212). it is now reported that upon incubation of the latter with rat liver microsomes, [14c-galactose]-gal1man9glcnac2-p-p-dolichol and [14c-galactose]gal1glc1man9glcnac2-p-p-dolichol are formed. the galactosyl residues appeared to be (1,3)-linked in the sam ... | 1988 | 2829724 |
| [spontaneous mutations in acetic acid bacteria. i. mutations in antibiotic resistance]. | | 1967 | 4299399 |
| the effect of glucose on the electron-transport systems of the aerobic bacteria azotobactervinelandii and acetobacter suboxydans. | | 1969 | 4306852 |
| enzymatic removal of diacetyl from beer. ii. further studies on the use of diacetyl reductase. | diacetyl removal from beer was studied with whole cells and crude enzyme extracts of yeasts and bacteria. cells of streptococcus diacetilactis 18-16 destroyed diacetyl in solutions at a rate almost equal to that achieved by the addition of whole yeast cells. yeast cells impregnated in a diatomaceous earth filter bed removed all diacetyl from solutions percolated through the bed. undialyzed crude enzyme extracts from yeast cells removed diacetyl very slowly from beer at its normal ph (4.1); at a ... | 1970 | 4315861 |
| characterization and properties of the pyruvate phosphorylation system of acetobacter xylinum. | the enzyme responsible for the direct phosphorylation of pyruvate during gluconeogenesis in acetobacter xylinum has been purified 46-fold from ultrasonic extracts and freed from interfering enzyme activities. the enzyme was shown to catalyze the reversible mg(2+) ion-dependent conversion of equimolar amounts of pyruvate, adenosine triphosphate (atp), and orthophosphate (p(i)) into phosphoenolpyruvate (pep), adenosine monophosphate (amp), and pyrophosphate (pp). the optimal ph for pep synthesis w ... | 1970 | 4319721 |
| pyrroloquinoline quinone: excretion by methylotrophs and growth stimulation for microorganisms. | a marked excretion of pyrroloquinoline quinone (pqq) by methylotrophs into the culture medium was observed when incubation was prolonged to the late stationary phase. when the organisms were growing vigorously in the early exponential phase, accumulation of pqq was repressed at a low level. some evidence was obtained that the excretion of pqq is related to turnover of quinoproteins of the organisms. the growth stimulation of microorganisms by pqq was demonstrated using acetobacter aceti. the pre ... | 1988 | 2855583 |
| reactivity with oxygen of bacterial cytochrome oxidases a1, aa3 and o. | | 1973 | 4352929 |
| role of carbon monoxide dehydrogenase in acetate synthesis by the acetogenic bacterium, acetobacterium woodii. | carbon monoxide dehydrogenase (codh) plays a key role in acetate synthesis by the acetogenic bacterium, clostridium thermoaceticum. acetobacterium woodii, like c. thermoaceticum contains high levels of codh. in this work we show that crude extracts of a. woodii synthesize acetate from methyl tetrahydrofolate or methyl iodide, carbon monoxide and coenzyme a (coa). the purified codh from a. woodii catalyzes an exchange reaction between co and the carbonyl group of acetyl-coa even faster than the c ... | 1988 | 2855585 |
| conjugative transfer of the naturally occurring plasmids of acetobacter xylinum by incp-plasmid-mediated mobilization. | broad-host-range plasmids and cloning vectors were conjugatively transferred to acetobacter xylinum. one of the plasmids, rp4::mu cts61, was used for the insertion of tn1 into the 16-, 44-, and 64-kilobase-pair plasmids of a. xylinum. the tn1-labeled plasmids could be mobilized by a helper plasmid. many of the tn1 insertions affected the copy number of the plasmids. | 1986 | 3001030 |
| growth inhibition of acetobacter aceti by l-threonine and l-homoserine: the primary regulation of the biosynthesis of amino acids of the aspartate family. | | 1974 | 4373525 |
| the plasmids of acetobacter xylinum and their interaction with the host chromosome. | acetobacter xylinum contains a complex system of plasmid dna molecules. plasmids of molecular weights or copy numbers different from the original wild-type, are found in different types of mutants. restriction endonuclease digestion and dna/dna hybridization analysis, showed that the plasmids often contained partly, but not completely the same dna sequences. two of these plasmid classes were analysed in more detail, and could be shown to differ in size by about 5 kb. hybridization analysis using ... | 1987 | 3039311 |
| [the synthesis and beta-lactamase inhibition activity of p-(3-amido-4-substituted phenyl-2-azetidinonyl-1)-phenylacetic acids and p-(3-amido-4-substituted phenyl-2-azetidinonyl-1)-acetophenones]. | | 1988 | 3138889 |
| comparative aspects of some bacterial dehydrogenases and transhydrogenases. | ragland, t. e. (brandeis university, waltham, mass.), t. kawasaki, and j. m. lowenstein. comparative aspects of some bacterial dehydrogenases and transhydrogenases. j. bacteriol. 91:236-244. 1966.-twenty-eight diverse bacterial species were surveyed for the activities and coenzyme specificities of four enzymes: isocitrate dehydrogenase (icdh), glucose-6-phosphate dehydrogenase (g-6-pdh), 6-phosphogluconate dehydrogenase (6-pgdh), and reduced nicotinamide adenine dinucleotide phosphate-nicotinami ... | 1966 | 4379210 |
| 5-keto-d-fructose. iv. a specific reduced nicotinamide adenine dinucleotide phosphate-linked reductase from gluconobacter cerinus. | | 1966 | 4379259 |
| [enzymatic activities of acetobacter suboxydans. effect of ph on the 5-ketogenic activity of subcellular fractions]. | | 1964 | 4381607 |
| preparation and characterization of monoclonal antibodies to cephalexin-synthesizing enzyme from acetobacter turbidans. | eleven monoclonal antibodies against the cephalexin-synthesizing enzyme have been constructed and primarily characterized. these antibodies are all igg1 type, with medium affinity, and with no enzyme-inhibition effect. they will be utilized as immunoadsorbents to purify their corresponding antigen, the enzyme, in one step. | 1988 | 3169806 |
| coenzyme linked dehydrogenases of acetic acid bacteria. | | 1969 | 4392346 |
| [regulation of the glucose-6-phosphate dehydrogenase of different bacterial species by atp]. | | 1969 | 4393658 |
| [demonstration of several alcohol dehydrogenases bound to nad and nadp respectively in acetobacter mesoxydans]. | | 1972 | 4400906 |
| intracellular ph and membrane potential as regulators in the prokaryotic cell. | | 1987 | 3295250 |
| a study of predominant aerobic microflora of black bears (ursus americanus) and grizzly bears (ursus arctos) in northwestern alberta. | swab specimens were obtained from nasal, rectal, and preputial or vaginal areas of 37 grizzly and 17 black bears, captured during may to june of 1981 to 1983, to determine the types and frequency of predominant aerobic microflora. bacterial genera most frequently isolated from bears were escherichia, citrobacter, hafnia, proteus, staphylococcus, and streptococcus species, comprising about 65% of the isolates. erwinia, xanthomonas, agrobacterium, rhizobium, and gluconobacter/acetobacter were also ... | 1987 | 3447691 |
| thiol and amino analogues as alternate substrates for glycerokinase from candida mycoderma. | the kinetic and catalytic mechanism of glycerokinase from candida mycoderma was examined with thiol and amino analogues of glycerol and with mgamppcp, an analogue of mgatp. (s)-1-aminopropanediol was phosphorylated on nitrogen (vmax 0.4% that of glycerol) while the r enantiomer was phosphorylated on oxygen (vmax 0.7% that of glycerol). (s)-1-mercaptopropanediol was phosphorylated on oxygen (vmax 3.5% that of glycerol), while the r enantiomer was phosphorylated on sulfur (vmax 0.001% that of glyc ... | 1989 | 2550062 |
| naturally occurring monobactams. | | 1986 | 3521210 |
| [numerical taxonomy of pseudomonads of the diminuta group]. | the taxonomy of the "diminuta" group is discussed. the method of numerical taxonomy was used to characterize 135 strains of 10 species belonging to pseudomonas, gluconobacter and acetobacter in terms of sixty phenotypical features. the similarity coefficients of the strains were calculated with computers. according to the data of numerical analysis, the species p. diminuta and p. vesiculare represent a single phenon different from pseudomonas, gluconobacter and acetobacter species. | 1986 | 3702780 |
| cellulose microfibril assembly and orientation: recent developments. | a brief history of the literature dealing with cellulose microfibril assembly is presented, and a current summary of cellulose microfibril synthesizing complexes among eukaryotic cells is given. terminal complexes not described before include the following: linear terminal complexes (tcs) with three rows in eremosphaera, microdictyon and chaetomorpha; globular terminal complexes in ophioglossum, psilotum, equisetum and gingko. cellulose microfibril assembly in acetobacter xylinum is described ve ... | 1985 | 3867669 |
| single-carbon chemistry of acetogenic and methanogenic bacteria. | methanogenic and acetogenic bacteria metabolize carbon monoxide, methanol, formate, hydrogen and carbon dioxide gases and, in the case of certain methanogens, acetate, by single-carbon (c1) biochemical mechanisms. many of these reactions occur while the c1 compounds are linked to pteridine derivatives and tetrapyrrole coenzymes, including corrinoids, which are used to generate, reduce, or carbonylate methyl groups. several metalloenzymes, including a nickel-containing carbon monoxide dehydrogena ... | 1985 | 3919443 |
| prokaryotic triterpenoids. 1. 3 beta-methylhopanoids from acetobacter species and methylococcus capsulatus. | 3 beta-methylbacteriohopanepolyol derivatives were isolated from three bacteria, acetobacter pasteurianus ssp. pasteurianus, methylococcus capsulatus and nostoc muscorum, and identified by spectroscopic methods and direct comparison with 3 beta-methyldiplopterol and 3 beta-methylhopan-29-ol synthesized from 22-hydroxyhopan-3-one. the 3 beta-methylhopanoid content of a. pasteurianus ssp. pasteurianus could be dramatically increased (up to 60% of the total hopanoid content) by addition of l-methio ... | 1985 | 3926494 |
| gluconic acid-producing bacteria from honey bees and ripening honey. | | 1973 | 4579122 |
| the structure of spherulites of bacterial cellulose from acetobacter xylinum. | | 1974 | 4597644 |
| polysaccharide biosynthesis in acetobacter xylinum. enzymatic synthesis of lipid diphosphate and monophospate sugars. | | 1974 | 4600325 |
| [thin layer electrophoretic determination of biogenic amines in fishes and fish products in connection with food poisoning]. | | 1974 | 4616180 |
| absorption bands of multiple b and c cytochromes in bacteria detected by numerical analysis of absorption spectra. | | 1972 | 4625502 |
| nonfunctional tricarboxylic acid cycle and the mechanism of glutamate biosynthesis in acetobacter suboxydans. | acetobacter suboxydans does not contain an active tricarboxylic acid cycle, yet two pathways have been suggested for glutamate synthesis from acetate catalyzed by cell extracts: a partial tricarboxylic acid cycle following an initial condensation of oxalacetate and acetyl coenzyme a. and the citramalate-mesaconate pathway following an initial condensation of pyruvate and acetyl coenzyme a. to determine which pathway functions in growing cells, acetate-1-(14)c was added to a culture growing in mi ... | 1972 | 4640504 |
| prokaryotic triterpenoids. 3. the biosynthesis of 2 beta-methylhopanoids and 3 beta-methylhopanoids of methylobacterium organophilum and acetobacter pasteurianus ssp. pasteurianus. | the incorporation of l-[methyl-3h,14c]methionine or l-(methyl-2h3)methionine into 2 beta-methyldiplopterol of methylobacterium organophilum and various 3 beta-methylhopanoids of acetobacter pasteurianus ssp. pasteurianus showed that all three hydrogen atoms of the transferred methyl group are retained in the triterpenoids. these methylations are compatible with a methylation substrate such as a delta 2-hopanoid in the case of the 2 beta-methylhopanoid biosynthesis and of a delta 2-hopanoid or sq ... | 1985 | 3926496 |
| nucleotide sequence of the membrane-bound aldehyde dehydrogenase gene from acetobacter polyoxogenes. | the nucleotide sequence of the membrane-bound aldehyde dehydrogenase (aldh) gene from an industrial vinegar producer, acetobacter polyoxogenes, was determined. comparison of the sequence with the nh2-terminal amino acid sequence of the mature aldh and determination of the actual translational initiation codon by means of in vitro manipulation of the upstream and proximal regions of the cloned gene showed that aldh was primarily translated as a 773-amino-acid protein and that the 44-amino-acid se ... | 1989 | 2606906 |
| studies on microbial transformation. xxvi. microbial oxidation of (-)-sparteine. | | 1973 | 4711516 |
| [biochemical dehydrogenations of saccharides. 6. notes on the dehydrogenation of l-arabitol by acetobacter xylinum and on bertrand's rule]. | | 1973 | 4740801 |
| effects of detergents and phospholipids on the pyridine nucleotide-independent aldehyde dehydrogenase from membranes of acetobacter rancens. | the pyridine nucleotide-independent aldehyde dehydrogenase solubilized and purified from membranes of acetobacter rancens ccm 1774 requires the presence of detergents for activity. while several detergents could stimulate the enzyme activity the stability of the enzyme-detergent complexes was rather low. phospholipid substitution experiments revealed the reversibility of the loss of activity caused by phospholipid removal. enzyme-phospholipid complexes generated from a complex phospholipid fract ... | 1985 | 4084277 |
| gastric erosions caused by home-brewed lager. | | 1971 | 4106609 |
| [detection of 2 forms of nad-dependent sorbitol dehydrogenase in acetobacter melanogenum]. | | 1973 | 4780707 |
| fine structural changes of acetobacter suboxydans during growth in a defined medium. | cytological differences were observed between stationary- and exponentialphase cells of acetobacter suboxydans grown in a defined medium. unstained cells observed with the light microscope just after entering the stationary phase differed from exponentially growing cells in that the former exhibited localized increases in density, particularly in the polar regions. electron microscopy of thin sections revealed that early stationary-phase cells possessed predominantly polar complexes of intracyto ... | 1973 | 4120607 |
| myo-inositol dehydrogenase(s) from acetomonas oxydans. optimization of conditions for solubilization of membrane-bound enzyme. | methods are described for the first reported successful isolation of the soluble form of the membrane-bound myo-inositol dehydrogenase(s) from acetomonas oxydans. conditions for optimum yields of active enzyme in a crude membrane-free protein extract were established. the relevant conditions are (a) cell rupture by solid-shearing, (b) solubilization of the complete 60000g pellet with sodium deoxycholate at ph7.2, (c) rapid separation of the released protein from sodium deoxycholate by gel chroma ... | 1974 | 4214535 |
| factors afecting the activity of pyruvate kinase of acetobacter xylinum. | 1. extracts of acetobacter xylinum were found to contain the glycolytic enzymes involved in the conversion of triose phosphate into pyruvate. pyruvate kinase had the lowest relative activity. phosphofructokinase activity was not detected in the extracts. 2. only slight differences in the activity of pyruvate kinase were observed between cells grown on glucose and those grown on intermediates of the tricarboxylic acid cycle. 3. pyruvate kinase, partially purified from ultrasonic extracts by ammon ... | 1969 | 4241738 |
| synthesis and kinase phosphorylation of 4-deoxy-d-threohexulose. | | 1973 | 4269357 |
| long-term storage of acetic acid bacteria by means of lyophilization. | | 1969 | 4912749 |
| fermentation processes employed in vitamin c synthesis. | | 1970 | 4920194 |
| professor t.k. walker. | | 1970 | 4922693 |
| [utilization by microorganisms of single-carbon compounds]. | | 1971 | 4949198 |
| the isolation and morphology of some new bacteriophages specific for bacillus and acetobacter species. | | 1965 | 4956049 |
| [on the acetic bacteria isolated from grapes]. | | 1969 | 4981161 |