| [campylobacter jejuni, yersinia enterocolitica and salmonella in game and poultry]. | samples of various sorts of game and poultry (other than chicken), obtained from poulterers, hunters, rearing-farms and slaughterhouses, were surveyed for the presence of campylobacter jejuni, yersinia enterocolitica and salmonella. these pathogens were isolated from hare, wild boars, pheasants, guinea-fowl, turkeys, mallards and domestic ducks. yersinia enterocolitica and salmonella were also cultured from wild and domestic rabbits. moreover, yersinia enterocolitica was isolated from wood pigeo ... | 1983 | 6648946 |
| pathogenicity of campylobacter jejuni and campylobacter coli strains in the pregnant guinea pig model. | pathogenicity of 17 campylobacter isolates for pregnant guinea pigs was investigated. of 14 isolates, 12 (86%) produced rates of abortion ranging from 13% to 87%. two isolates did not produce abortion. reference strains of c fetus subsp venerealis produced abortion in 60% to 87% and c fetus subsp fetus produced abortion in 60% of the guinea pigs. inoculated organisms were recovered from uterus, blood, liver, kidney, spleen, and gallbladder of the guinea pigs at rates as high as 83% for 2 ovine i ... | 1983 | 6650962 |
| isolation and enumeration of campylobacter jejuni from poultry products by a selective enrichment method. | a direct selective enrichment procedure was developed for the isolation of campylobacter jejuni from poultry products. the selective enrichment medium (atb) consisted of (per liter) tryptose (20 g), yeast extract (2.5 g), sodium chloride (5 g), fbp supplement (ferrous sulfate [0.25 g], sodium metabisulfite [0.25 g], sodium pyruvate [0.25 g]), bicine (10 g), and agar (1 g). hematin solution (6.25 ml; prepared by dissolving 0.032 g of bovine hemin in 10 ml of 0.15 n sodium hydroxide solution and a ... | 1983 | 6651294 |
| effect of temperature, duration of incubation, and ph of enrichment culture on the recovery of campylobacter jejuni from eviscerated market chickens. | to establish an enrichment system of high efficiency for recovery of campylobacter jejuni from market chickens, the effects of the temperature, duration of incubation, and ph of the enrichment culture on the isolation of the bacterium were evaluated. whole chickens or chicken parts in plastic bags were individually rinsed, and the washings filtered through cheesecloth. the cells were separated from the washings by centrifugation, and the pellet was inoculated into 100 ml of enrichment broth. iso ... | 1983 | 6684497 |
| endemic campylobacter jejuni infection in colorado: identified risk factors. | a study of persons with laboratory-confirmed sporadic campylobacter jejuni infection and of controls matched for age and sex in colorado in the summer of 1981 yielded odds ratio estimates significantly greater than 1 for the following risk factors: drinking raw water (10.74), drinking raw milk (6.93), eating undercooked chicken (2.77), and living in a household with a cat (3.21). | 1984 | 6696155 |
| campylobacter jejuni diarrhea model in infant chickens. | to study the pathogenic mechanisms of campylobacter jejuni infection, 36- to 72-h-old chickens were fed 10(3) to 10(6) live cells, using strains isolated from 40 patients with watery diarrhea and 6 with bloody mucoid diarrhea from whom no other known enteropathogen was detected. chickens of starbro strain were more likely to develop c. jejuni-induced diarrhea than were white leghorn chickens. diarrhea was defined on the basis of amounts of gut fluid in 288 chicks fed with live c. jejuni versus 1 ... | 1984 | 6698612 |
| comparative studies on competitive exclusion of three isolates of campylobacter fetus subsp. jejuni in chickens by native gut microflora. | resistance of young chicks to campylobacter fetus subsp. jejuni was substantially increased by early exposure to native gut microflora. protection was demonstrated against two human isolates and a chicken isolate of c. fetus subsp. jejuni. significant protection against the chicken isolate was observed throughout a 91-day test period. infection reached 100% (25/25) in the untreated group at 56 days of age and only 4% (1/25) in the group treated with native gut microflora. campylobacter fetus sub ... | 1984 | 6721792 |
| a study of the spread of campylobacter jejuni in four large kitchens. | campylobacters were sought in swabs taken from work surfaces, sinks and floors of four kitchens-i.e. hospital, university, cook-freeze and commercial, processing frozen or fresh chickens. each kitchen was visited on four occasions. in the large commercial kitchen environmental contamination was found on each visit, whereas campylobacters were isolated on six of the twelve visits to the other kitchens. the hands of operatives were contaminated with campylobacters on only two of the 45 swabs taken ... | 1984 | 6736643 |
| occurrence of campylobacter jejuni in country chicken in calcutta. | | 1984 | 6746032 |
| systematic investigation of enrichment media for wild-type campylobacter jejuni strains. | of the media examined, thioglycolate broth supplemented with 5% lysed sheep blood, butzler antibiotic mixture, and 0.1% lauryl sulfate was the most sensitive enrichment medium for recovery of wild-type strains of campylobacter jejuni from cecal contents of chickens and chicken livers. it allowed the retrieval of 1 cfu as did solid media but permitted the screening of 50-times larger volumes. double-strength enrichment medium required 5 to 10 cfu for growth. omission of lauryl sulfate reduced the ... | 1984 | 6746890 |
| campylobacter jejuni survival in chicken meat as a function of temperature. | recognition of campylobacter fetus subsp. jejuni (referred to hereafter as c. jejuni) as an important human pathogen and its isolation from meat products indicate the need for knowledge of its survival characteristics in meats. thermal death times (d-values) for a single strain and a five-strain composite were determined in 1% peptone and autoclaved ground chicken meat at temperatures ranging from 49 to 57 degrees c. survival was determined for these strains in chicken meat at 4, 23, 37, and 43 ... | 1982 | 6812501 |
| prevalence of campylobacter jejuni in two california chicken processing plants. | two federally inspected california chicken processing plants participated in campylobacter jejuni prevalence studies. twelve sampling sites were included in each of four groups. groups were based on bird age, scald water temperature, and plant sampled. scald water temperatures of 60 degrees c (140 degrees f) did not contribute to a lower prevalence of c. jejuni in edible parts, as did temperatures of 53 degrees c (127 degrees f) and 49 degrees c (120 degrees f). the feather picker and chilling t ... | 1983 | 6830212 |
| house flies (musca domestica) as possible vectors of campylobacter fetus subsp. jejuni. | a total of 161 strains of campylobacter fetus subsp. jejuni were isolated from house flies (musca domestica). the carrier rates detected were 50.7% in flies captured on a chicken farm and 43.2% in flies from a piggery. the relative prevalences of campylobacter coli, c. jejuni, and nalidixic acid-resistant thermophilic campylobacters were 90.1, 6.2, and 3.7%, respectively. the results indicate that flies may play a linking role in the epidemiology of campylobacter infection in humans by transmitt ... | 1983 | 6830213 |
| prevalence of campylobacter jejuni in chicken wings. | campylobacter jejuni was found in 82.9% of 94 chicken wing packages analyzed on the day of arrival at supermarkets and in 15.5% of 45 packages obtained from the supermarket shelves a few days later. the number of bacterial cells ranged from 10(2) to 10(3.9) per wing. the prevalence of c. jejuni in the wings varied with the brand, the day of sampling, and the age of the product. | 1983 | 6847181 |
| location of campylobacter jejuni in infected chicken livers. | to determine whether chicken livers infected with campylobacter jejuni are seeded in vivo or contaminated after slaughtering, 117 livers purchased in retail outlets in new york were examined for surface and tissue infections. of 56 livers positive for c. jejuni, 36 yielded surface growth only, 18 both surface and tissue growth, and 2 tissue growth only. the scanty growth from tissue samples suggests a carry-over of organisms from the surface. it was concluded, therefore, that contamination is mo ... | 1983 | 6863511 |
| enteropathogenic bacteria in frozen chicken. | eighty-two samples of frozen chicken from retail stores were examined for the presence of campylobacter, yersinia enterocolitica, and salmonellae. aerobic plate counts and numbers of coliform bacteria at 37 degrees c were determined. campylobacter fetus subsp. jejuni was found in 22% of the samples, y. enterocolitica was found in 24.5% and salmonella typhimurium was found in one sample (1.2%). the isolated strains of y. enterocolitica belonged to serotypes 4, 5b, 6, and 8. aerobic plate counts a ... | 1981 | 7020597 |
| serotyping of campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. | a serotyping scheme for campylobacter jejuni was developed based on slide agglutination of live bacteria with whole cell antisera absorbed with homologous heated and heterologous unheated cross-reactive antigens. among 815 isolates from human and nonhuman sources, 21 serogroups were recognized. of the 615 isolates from human cases of gastroenteritis, 529 (86%) were typable; 455 strains agglutinated in 20 single antisera, whereas 74 isolates agglutinated in various pairs of antisera, allowing sub ... | 1982 | 7096555 |
| epidemiological aspects of campylobacter jejuni enteritis. | an epidemiological study on campylobacter jejuni enterocolitis was performed in an urban swedish community. the study included 55 patients gathered during a six-month period. forty-one of the 55 patients (75%) were infected outside sweden. campylobacter enterocolitis was rare among children within the country. patients infected in sweden had eaten chicken significantly more often than a corresponding control group. seven out of nine chicken consuming campylobacter patients also had prepared the ... | 1982 | 7097000 |
| recovery of campylobacter jejuni and campylobacter coli from inoculated foods by selective enrichment. | a direct enrichment procedure was developed to selectively recover small numbers of campylobacter jejuni, c. coli, and nalidixic acid-resistant thermophilic campylobacter from foods. the procedure includes an enrichment medium composed of brucella broth, 7% lysed horse blood, 0.3% sodium succinate, 0.01% cysteine hydrochloride, vancomycin (15 micrograms/ml), trimethoprim (5 micrograms/ml), polymyxin b (20 iu/ml), and cycloheximide (50 micrograms/ml) that is inoculated with 10 or 25 g of food and ... | 1982 | 7103488 |
| pathogenicity of campylobacter jejuni isolates from animals and humans. | fourteen isolates of campylobacter jejuni of different serotypes and one campylobacter coli isolate, from various human and animal sources, were tested for potential pathogenic mechanisms. enterotoxin production was not detected in the infant mouse test or by calf and piglet ligated intestinal loop studies. isolates were not invasive by the sereny test. all isolates associated with and penetrated hela cells, although both actions occurred generally in a minor way under the conditions of our stud ... | 1982 | 7141696 |
| intestinal colonization and competitive exclusion of campylobacter fetus subsp. jejuni in young chicks. | colonization of campylobacter fetus subsp. jejuni was investigated in monoxenic and holoxenic chicks. in monoxenic chicks, major colonization was found in the crop and ceca, with populations in the ceca consistently reaching 10(9) colony-forming-units/ml of cecal contents over the 28-day test period. bacteremia was found in most chicks, but no significant gross pathological lesions were detected. in holoxenic chicks, major colonization occurred only in the ceca, and no evidence of bacteremia was ... | 1982 | 7150145 |
| campylobacter jejuni contamination of slaughtered chickens. | gut samples from 50 nonselected slaughtered chickens were obtained in two poultry processing plants and cultured for campylobacter jejuni and salmonella. positive results were obtained in 84% and 4%, respectively. viable c. jejuni and salmonella were detected in every phase of processing, even during packaging for commercial purposes. of surface samples taken from 118 slaughtered chickens prepared for delivery to consumers, 88 were contaminated by c. jejuni and 17 by salmonella. | 1982 | 7168372 |
| effect of recovery medium on the isolation of campylobacter jejuni before and after heat treatment. | | 1982 | 7180785 |
| campylobacter jejuni: incidence in processed broilers and biotype distribution in human and broiler isolates. | campylobacter jejuni was isolated from 18 of 40 processed broiler carcasses and 134 of 327 cloacal swabs obtained at four processing plants in sydney, australia. three of four flocks examined carried c. jejuni. eighty-two percent of chicken and 98% of human isolates from the area were of identical biotypes. | 1982 | 7201780 |
| carriage of campylobacter jejuni in healthy and diarrheic animals. | feces from normal and diarrheic animals were cultured for campylobacter jejuni. a clear difference could not be detected in carriage between normal and diarrheic cattle, horses, pigs, and dogs. too few diarrheic goats, sheep, and rabbits were sampled for conclusions to be made. carriage rates (%) detected in normal animals were as follows: ducks 88.3, chickens 23.8, sheep 13.6, rabbits 11.3, goats 2.7, cattle 2.5, and dogs 0.5. the organism was not isolated from horses and mink. carriage rates v ... | 1981 | 7224312 |
| antimicrobial susceptibility of campylobacter jejuni isolated from humans with diarrhoea and from healthy chickens. | | 1981 | 7275880 |
| incidence of campylobacter jejuni in fresh eviscerated whole market chickens. | | 1981 | 7296414 |
| experimental campylobacter diarrhea in chickens. | an animal model for campylobacter fetus subsp. jejuni enteritis was developed in 3-day-old chickens. diarrhea was induced in 88% (22 of 25) of chickens inoculated with 9 x 10(7) bacteria given orally. the mean incubation time was 45 h (range, 24 to 72 h). considerable weight loss was observed in the experimental group compared with the control group. ninety bacteria was the minimal infective dose capable of inducing diarrhea in 90% of the chickens. overall mortality was 32% (8 of 25). light micr ... | 1981 | 7298187 |
| the occurrence of campylobacter jejuni in fresh food and survival under different conditions. | campylobacter jejuni was an almost regular finding in chickens and in minced meat from pigs and cattle sold in ordinary food stores. the bacteria survived on the food at 4 degrees c for one week and frozen at -20 degrees c for three months. none of the strains tested survived heat treatment at 60 degrees c for longer than 15 min. c. jejuni is apparently a frequent guest in most kitchens. correct food handling and heat treatment to at least 60 degrees c for 15 min should be enough to prevent infe ... | 1981 | 7310124 |
| gastroenteritis due to campylobacter jejuni in riyadh, saudi arabia. | during one year, 1452 specimens of faeces from patients with diarrhoea were investigated for campylobacter jejuni. this organism was isolated from nine specimens only (0.6%). none was isolated from 160 control subjects who did not have diarrhoea. though the number of isolates is small, diarrhoea due to c. jejuni was found to be more common in males; the ratio of males to females was 7:2. out of the nine patients, only four were saudis. this indicates that campylobacter gastroenteritis exists in ... | 1981 | 7324103 |
| occurrence of campylobacter fetus subsp. jejuni and yersinia enterocolitica in domestic animals and in some foods of animal origin in finland. | fecal samples of some domestic animals were investigated for occurrence of c. fetus subsp. jejuni and y. enterocolitica. the findings in this survey suggest, that the contamination rate in domestic animals for both bacteria is low. raw milk, pasteurized milk or ground meat samples were not positive for c. fetus subsp. jejuni. four per cent of the raw milk samples were positive for y. enterocolitica. each of the y. enterocolitica strains belonged to nilehn's biotype 1 and they were noninvasive. | 1981 | 7329785 |
| isolation of campylobacter jejuni from poultry carcases. | campylobacter jejuni was isolated from 94% of chicken carcase rinses collected in a queensland poultry abattoir. direct plating resulted in the isolation of the organism from 84% of the rinses. a further 10% were found to contain c. jejuni by a procedure of selective enrichment and membrane filtration. counts of c. jejuni on the carcases ranged from 0 to 1.1 x 10(5) organisms per carcase. | 1981 | 7342935 |
| broiler chickens as potential source of campylobacter infections in humans. | of 46 broiler chickens from a live poultry market in new york city, 38 (83%) harbored campylobacter fetus subsp. jejuni in their rectal flora. the observed mean number of c. fetus per g of feces was 4.4 x 10(6). the organisms survived in the feces for at least 96 h at 4 degrees c whether stored in the gut or transferred to a vial. the best survival medium for pure cultures of c. fetus subsp.jejuni was heart infusion broth supplemented with sterile blood and kept in a microaerophilic atmosphere. | 1980 | 7381018 |
| diffusion of thermophilic campylobacter in the pesaro-urbino area (italy) from 1985 to 1992. | the results of research on the spreading of campylobacter in the pesaro-urbino area carried out from 1985 to 1992 are presented. materials of different origin were examined: 822 samples of human faeces, 533 animal rectal swabs, 192 samples of domestic sewage, 48 of river water, 96 of sea water and 632 of various types of food. two hundred and nine strains of campylobacter were isolated (9%), most of which were campylobacter jejuni (80%), with particular frequency in food products (chicken carcas ... | 1995 | 7489778 |
| isotype, specificity, and kinetics of systemic and mucosal antibodies to campylobacter jejuni antigens, including flagellin, during experimental oral infections of chickens. | the immune response of chickens to campylobacter jejuni infection was studied as a step in the search for vaccine candidates. one-day-old chicks orally challenged with c. jejuni strain 81116 showed significant increases in specific igg, iga, and igm circulating antibodies, as detected by enzyme-linked immunosorbent assay (elisa). these levels peaked at 9, 5, and 7 weeks postinfection, respectively. maternal igg antibodies were also detected over the first 2 weeks. specific mucosal igg and iga an ... | 1994 | 7526839 |
| high-resolution genotyping of campylobacter coli identifies clones of epidemiologic and evolutionary significance. | campylobacter coli strains from clinical and other sources were examined in terms of o (heat-stabile; hs) serotype and by several molecular typing techniques. restriction fragment length polymorphism (rflp) around the three 16s rrna genes revealed 10 variants, none found in campylobacter jejuni. rflp analysis of a polymerase chain reaction amplicon generated from the flagellin gene (flaa) yielded 11 polymorphism groups, some of them linked to hs serotypes. enlarged flaa genes, contributing three ... | 1995 | 7561196 |
| prevalence of thermotolerant species of campylobacter and their biotypes in children and domestic birds and dogs in southern chile. | the prevalence of thermotolerant campylobacter in diarrhoeic and healthy children as well as in dogs, hens, ducks and pigeons was determined in southern chile. campylobacter were found in 34.5% of the faecal samples examined. the isolation rate of campylobacter in diarrhoeic and healthy children was 16.3% and 6.4% respectively. despite c. jejuni was always more frequent than c. coli, the latter was isolated with a high frequency (29%) from patients with diarrhoea. c. jejuni and c. coli biotypes ... | 1994 | 7569610 |
| dna diversity among isolates of campylobacter jejuni detected by pcr-based rapd fingerprinting. | a pcr-based randomly amplified polymorphic dna method was used to amplify campylobacter jejuni dna using a single oligonucleotide primer derived from either a homologous source or from mycoplasma gallisepticum. the method was able to detect the heterogeneity of amplified dna from human, chicken and turkey sources and can be used as a tool to study the epidemiology of campylobacter jejuni infection. | 1995 | 7571378 |
| effect of the complement system on the sensitivity of campylobacter jejuni and campylobacter coli to human blood serum. | we determined the sensitivity of five strains of campylobacter jejuni and c. coli isolated from children with diarrhea and from chicken feces to normal human blood serum (undiluted and at concentrations of 10, 30, 50 and 70%), a hypogammaglobulinemic serum and a complement-deficient serum. both species were highly sensitive to the bactericidal activity of human serum, regardless of their source. the highest bactericidal activity was observed with pooled fresh normal serum, with bacterial surviva ... | 1995 | 7581046 |
| detection of genomic variability among isolates of campylobacter jejuni from chickens by crossed-field gel electrophoresis. | digestion with sal i facilitated the subclassification of 41 strains of campylobacter jejuni into seven types, and digestion with sma i enabled subclassification into twelve types. sma i was potentially more useful for the detection of variability among the 41 strains, but both restriction enzymes seemed to be potentially useful for detecting variability among crossed-field gel electrophoresis profiles of the strains. the results clearly demonstrated that c. jejuni strains from different sources ... | 1995 | 7587375 |
| differentiation of campylobacter jejuni and campylobacter coli strains by using restriction endonuclease dna profiles and dna fragment polymorphisms. | the chromosomal dna fragment patterns from a total of 169 campylobacter jejuni and campylobacter coli isolates from poultry and humans were analyzed by using dna restriction endonucleases clai and ecorv. the dna restriction patterns produced by clai and ecorv consisted of unique dna fragments of 9 to 9.5 kb and 3.5 kb generated with clai and a single unique fragment of 3.0 kb produced by ecorv. these patterns were obtained with all strains of c. jejuni tested. the dna restriction patterns were f ... | 1995 | 7615717 |
| campylobacter spp. in broilers on the farm and after transport. | colonization of the ceca and contamination on carcasses of chickens by campylobacter spp. was investigated. samples were taken on the farm and after transport and holding. in the first set of experiments, 20 chickens, obtained from each of 10 broiler farms, were collected from houses containing 6- to 7-wk-old birds. half of the birds were slaughtered at the farm; the other half were transported (10 birds per chicken coop) to a holding facility and killed within 16 to 18 h. the levels of campylob ... | 1995 | 7644422 |
| [infectivity of campylobacter jejuni and campylobacter coli in chickens]. | the infectivity of four campylobacter (c.) jejuni strains and one c. coli strain was evaluated by the reisolation rate from cloacal swabs after oral infection of chickens at four weeks of age. one strain did not colonize the digestive tract, another infected only a part of the experimental group and a third was excreted at a lower percentage in the first two weeks p.i. compared to the other two strains. in correlation to age, the excretion rate was highest in 7-week-old birds. in groups of 4 and ... | 1995 | 7646423 |
| [beta-lactamases of campylobacter spp. and their use as epidemiologic markers]. | the tests of 64 clinical strains of campylobacter jejuni and 69 strains isolated from hens revealed beta-lactamases in 96.99 per cent of the cultures. the detected enzymes more frequently splitted oxacillin and ampicillin among 5 betalactams used as the substrates (ampicillin, azlocillin, oxacillin, cefoperazon and cefotaxime). on the whole 24 spectra of the beta-lactamase activity with the evident domination of the amazoxcfpctx spectrum (29.69 per cent) were detected in both the clinical strain ... | 1994 | 7695448 |
| adhesion activity of campylobacter jejuni for intestinal epithelial cells and mucus and erythrocytes. | live and formalin- or heat (100 degrees c, 30 min)-treated cells of campylobacter jejuni strains y6878 and y6817, and the supernatant of the heat-treated bacterial suspension had agglutinating activity for the intestinal epithelial cells of japanese quails and human (int407). among the erythrocytes derived from 7 animal species (human, horses, sheep, rabbits, mice, chickens, and quails), only rabbit erythrocytes were agglutinated by live and glutaraldehyde-, formalin-, or heat-treated cells of 1 ... | 1994 | 7696403 |
| occurrence of plasmids and tetracycline resistance among campylobacter jejuni and campylobacter coli isolated from whole market chickens and clinical samples. | twenty whole market chickens, purchased from 10 different stores in the taipei metropolitan area, were examined for the presence of campylobacter jejuni and campylobacter coli. the microorganisms were recovered from 95% of the chickens. a survey of different sites on--breast, thigh and tail--showed that contamination was equally common on all these sites. one hundred and sixty-seven chicken isolates and the 41 clinical isolates of campylobacter jejuni were examined for the occurrence of plasmid ... | 1994 | 7703010 |
| detection of the coccoid form of campylobacter jejuni in chicken products with the use of the polymerase chain reaction. | detection of the coccoid form of campylobacter jejuni with the use of the polymerase chain reaction (pcr) was examined. coccoid cells of this pathogen, formed at different temperatures, showed different detection characteristics in the pcr. for spirals and cocci formed at 4 degrees c and 12 degrees c, the detection limit was about 2 x 10(3) cells/pcr. however, for detection of coccoid cells formed at 25 degrees c and 37 degrees c, at least 2 x 10(4) cells per pcr were needed. pcr was also perfor ... | 1994 | 7703020 |
| antimicrobial susceptibility and plasmid analysis of campylobacter jejuni isolated from diarrhoeal patients and healthy chickens in northern india. | seventy-five strains of campylobacter jejuni isolated from humans with diarrhoea (45 strains) and healthy chickens (30 strains) were tested for their susceptibility to different antimicrobial agents: ampicillin, tetracycline, erythromycin, gentamicin, kanamycin, furazolidine and quinolones (nalidixic acid, norfloxacin, ciprofloxacin). the frequencies of resistance to ampicillin and tetracycline were 16 and 9.3% respectively. two strains (2.7%) exhibited resistance to quinolones as mentioned. one ... | 1994 | 7751568 |
| specific detection of campylobacter jejuni by means of polymerase chain reaction in chicken litter. | a routine detection assay of campylobacter jejuni was developed with the polymerase chain reaction (pcr). a pair of oligonucleotide primers from the flaa of c. jejuni were used in detection by pcr. although the primer pair specifically detected c. jejuni strains, they did not detect strains of c. coli, c. fetus, salmonella spp. or escherichia coli. in litter inoculated with c. jejuni, specific detection of c. jejuni was obtained by pcr with this primer pair. | 1995 | 7756404 |
| restriction fragment length polymorphisms among the flagellar genes of the lior heat-labile serogroup reference strains and field strains of campylobacter jejuni and c. coli. | several typing systems have been described for campylobacter jejuni and c. coli, to assess the complex epidemiology of these important enteric pathogens. in the present study two typing methods, slide agglutination according to the lior scheme, and the demonstration of restriction-fragment length polymorphisms (rflp) of flagellar genes, have been used in parallel on a set of 194 strains. this set comprised 118 sero-reference strains of c. jejuni and c. coli of the lior scheme, as well as 76 clin ... | 1995 | 7781730 |
| discrepancy between penner serotyping and polymerase chain reaction fingerprinting of campylobacter isolated from poultry and other animal sources. | thirty-four campylobacter jejuni or coli strains, isolated from various livestock and darkling beetles from two dutch poultry farms during different broiler production cycles, were subjected to penner serotyping and polymerase chain reaction (pcr) fingerprint analysis. ten different penner serotypes were determined in the isolates. visual scoring of the pcr fingerprints resulted in 14 clearly different profiles. some strains with identical penner serotypes exhibited different pcr fingerprints an ... | 1995 | 7786504 |
| prevalence of campylobacter enteritis in children from yaounde (cameroon). | a cross sectional study on the prevalence of campylobacter jejuni/coli and its possible aetiologic role in childhood diarrhoea in yaounde was undertaken in 272 children presenting with diarrhoea and 157 age matched controls from april 1989 to october 1990. stool cultures were performed according to standard techniques for campylobacter jejuni/coli shigella and salmonella species. rotavirus was detected using a latex agglutination test. campylobacter jejuni/coli was isolated from 21 (7,7 pc) of 2 ... | 1995 | 7788677 |
| identification of campylobacter jejuni on the basis of a species-specific gene that encodes a membrane protein. | to facilitate discrimination between the closely related enteropathogens campylobacter jejuni and c. coli, unique differences in antigenic surface structure were examined. a genomic library of c. jejuni 81116 was constructed in plasmid pbluescriptiisk- and expressed in escherichia coli k-12. rabbit hyperimmune serum raised against c. jejuni atcc 29428 recognized a clone expressing a c. jejuni 24-kda membrane-associated protein. antiserum raised against sonicated recombinant e. coli expressing th ... | 1995 | 7790451 |
| experimental colonization of mice with campylobacter jejuni. | the ability of one human and two chicken strains of campylobacter jejuni to colonise and survive in three different strains of laboratory mice (nmri, cba and c57-black) was studied. mice were inoculated orally with campylobacter jejuni and faeces samples were cultured at regular intervals during the following months. the length of colonisation of mice differed between mouse strains but also between campylobacter strains. the mouse strain c57-black was not colonised with c. jejuni to the same deg ... | 1994 | 7801521 |
| flagellin gene polymorphism analysis of campylobacter jejuni infecting man and other hosts and comparison with biotyping and somatic antigen serotyping. | flagellin gene sequence polymorphisms were used to discriminate amongst 77 strains of campylobacter jejuni from sporadic and outbreak-associated human enteric infections, and from chickens, sheep and calves. the results were assessed in relation to lior biotyping and serotyping (penner somatic antigens). eight dna pcr-rflp patterns (genotypes) were identified by analysis of hinfi fragment length polymorphisms in flagellin gene (flaa) polymerase chain reaction (pcr) products. one genotype (f-1) w ... | 1994 | 7925661 |
| development of a tissue culture assay system for campylobacter jejuni cytotoxin and the influence of culture conditions on cytotoxin production. | a tissue culture assay system to detect campylobacter jejuni cytotoxin was developed, and the effect of culture conditions on cytotoxin production was examined. broth-culture filtrates from clinical isolates were used. the expression of cytotoxicity was dependent on the presence of serum in the culture medium. although the titre of cytotoxin was low, the greatest cytotoxicity was revealed when newborn calf (ncs), adult bovine or goat serum was added to minimum essential medium. moderate or weak ... | 1994 | 7932615 |
| persistent, recurring diarrhea in a colony of orangutans (pongo pygmaeus) caused by multiple strains of campylobacter spp. | a colony of 10 orangutans (pongo pygmaeus) experienced persistent, recurring diarrhea caused by multiple infections with campylobacter jejuni and c. coli. infections appeared to have occurred through several mechanisms, including fecal-oral transmission between orangutans, and possibly transmission by houseflies contaminated with the organisms from nearby chicken feces. among the 14 fecal and environmental c. jejuni isolates, 4 different antibiotic susceptibility profiles were detected; there we ... | 1994 | 7942349 |
| a survey of campylobacter jejuni in broilers from assignment to slaughter using dna-dna hybridization. | a survey of campylobacter jejuni in the cecal contents of broilers raised on a farm was carried out by the dna-dna hybridization method from the day of assignment to slaughter at about 1-week intervals. c. jejuni was detected in chickens as early as 1 week of age, and was widely detected at each week of age throughout the growing period. in addition, of 20 chickens tested just after assignment, 7 (35%) were c. jejuni positive. it is suggested that newly introduced chickens may have already been ... | 1994 | 7999894 |
| inhibition of campylobacter jejuni colonization in chicks by defined competitive exclusion bacteria. | campylobacter enteritis in humans has been linked to consumption of chicken. reducing campylobacter jejuni colonization in chickens can potentially reduce campylobacter infections in humans. in this study, the reduction of c. jejuni colonization in chicks by oral administration of defined competitive exclusion (ce) cultures, 2.5% dietary carbohydrates, or ce cultures and carbohydrates was examined. prevention, elimination, or direct challenge of campylobacter infection was simulated by administe ... | 1994 | 8017915 |
| effect of ionizing dose rate on the radioresistance of some food pathogenic bacteria. | food pathogenic bacteria including listeria monocytogenes (1a1 and atcc 19111), staphylococcus aureus (gd13 and atcc 13565), escherichia coli o157:h7 (atcc 35150), salmonella typhimurium, yersinia enterocolitica, vibrio parahaemolyticus, and campylobacter jejuni were exposed to various rates of ionizing radiation (0.78, 2.6, and 22 kgy/h) emitted by three different 60co irradiators. d10 values (d10 is the radiation dose required to eliminate 90% of a bacterial population (one logarithmic cycle r ... | 1994 | 8069779 |
| application of a dna-dna hybridization method for detection of campylobacter jejuni in chicken feces. | a direct colony hybridization method was used for the detection of campylobacter jejuni in chicken feces. the biotin-labeled dna prepared from the whole genome dna of c. jejuni subsp. jejuni atcc 33560 reacted well with homologous dna and slightly with c. coli dna. the method with the probe was found to be sensitive enough to detect a small number (10(2) cfu/g) of c. jejuni in chicken feces which contained a large number of background flora. it was suggested that this simple and sensitive method ... | 1993 | 8117799 |
| [isolation of campylobacter jejuni atcc 29428 from inoculated fried pork meat and roasted chicken]. | the human gastroenteritis caused by campylobacter jejuni in some industrialized countries is higher than gastroenteritis produced by salmonella and shigella. this has induced the development of techniques to demonstrate the presence of the microorganism in different foods using some culture media combinations. there is not a method to isolate c. jejuni from roasted chicken and fried pork meat, which are popular foods in méxico. the sensitivity of two culture media combinations was compared: rama ... | 1993 | 8140332 |
| an in-vitro meat model for the immediate bactericidal effect of lactic acid decontamination on meat surfaces. | an in-vitro model of the lactic acid decontamination (lad) of meat is described. as lad is a disinfection rather than a preservation process the model is based on the inactivation kinetics of bacteria in a suspension of pork skin. the model takes account of interfering factors present in nature, such as microbial interactions, leaching of organic material from the meat surfaces and buffering activity. | 1994 | 8144404 |
| mucosal competitive exclusion to diminish colonization of chickens by campylobacter jejuni. | mucosal competitive exclusion flora (mce) and length of mce culture storage were studied to determine their influence upon colonization of campylobacter jejuni in chickens. day-of-hatch broiler chicks, held in isolation units, were treated with mce or were left untreated. thirty to 48 h later, groups of chicks were challenged with serial dilutions of c. jejuni (strain a74/c). single challenge doses and treatments were administered to chickens within each isolation unit. after 5 d, chicks were ki ... | 1994 | 8177818 |
| efficacy of filter types for detecting campylobacter jejuni and campylobacter coli in environmental water samples by polymerase chain reaction. | a previously developed polymerase chain reaction (pcr) amplification of a target region in the flaa campylobacter flagellin gene was evaluated and adapted for use with environmental water samples. the ability to detect campylobacter jejuni or campylobacter coli in seeded water samples was tested with various filters after concentration and freeze-thaw lysis of the bacterial cells. a nonradioactive probe for the amplified flagellin gene fragment detected as little as 1 to 10 fg of genomic dna and ... | 1993 | 8285708 |
| colonization of chicks by motility mutants of campylobacter jejuni demonstrates the importance of flagellin a expression. | campylobacter jejuni strain 81116 contains two flagellin genes, flaa and flab. wild-type (wt) bacteria express flaa only, but flab can be expressed under certain conditions. we have determined the importance of flagella for colonization of the avian caecum, which appears to be the natural environment for these bacteria. mutants in which flaa or flab, or both had been inactivated, and motility variants, were investigated. flagella are not a requisite for colonization, but mutants lacking both fla ... | 1993 | 8360610 |
| a cytotoxicity test for the detection of campylobacter jejuni toxin. | a 7-h 51cr-release cytotoxicity assay has been developed to detect the presence of campylobacter jejuni toxin in the culture supernatant. some of the culture supernatants have cytotoxic effects against labeled chicken lymphocytes, while some others do not have this effect. there is no direct correlation between the pathogenicity of the organisms and the presence of the toxin. the supernatant of c. jejuni retains its cytotoxicity after heating at 100 degrees c for 10 min.; therefore, it is a heat ... | 1993 | 8362493 |
| frequency and enterotoxigenicity of campylobacter jejuni and c. coli in domestic animals in pakistan as compared to sweden. | domestic and wild animals have been considered to be the most common reservoirs of campylobacter for human infections. this study has been conducted to establish the frequency and enterotoxigenicity of c. jejuni and c. coli in the stools of domestic animals in a developing versus a developed country. faecal samples of 300 chickens and laying hens, 102 horses, 180 cows, 92 goats and sheep were collected from pakistan and sweden. the hippurate hydrolysis test and cho-cell test were performed for s ... | 1993 | 8429572 |
| effect of host lineage on the virulence of campylobacter jejuni/coli in the chick embryo model. | the chorioallantoic membrane (cam) inoculated chick embryo model was used to study the effect of host lineage on the virulence of campylobacter jejuni and campylobacter coli. ld50 values were used to compare the susceptibilities of chick embryos from eight inbred chicken lines to infection by four strains of c. jejuni and one strain of c. coli. differences in susceptibility were found between inbred chicken lines. these were shown not to be due to maternal antibody status, nor transfer of antibo ... | 1993 | 8454192 |
| the role of management systems in the epidemiology of thermophilic campylobacters among poultry in eastern zone of tanzania. | a total of 255 samples of droppings collected from a total of 22 different poultry units were examined for the presence of thermophilic campylobacters and the isolates biotyped using skirrow's protocol. the organisms were isolated from 90 (35.3%) of all samples. among the 22 units investigated, 13 (59%) were found to have unsatisfactory management systems, while 7 (32%) and 2 (9%) were found to have unsatisfactory and good systems respectively. significantly large numbers of isolations, 68 of 14 ... | 1993 | 8472771 |
| colonization of broiler chickens by waterborne campylobacter jejuni. | chickens on a broiler farm in southern england were found to be colonized with campylobacter jejuni of a single serotype, lior 1 penner 4. the farm was the sole supplier of a local slaughterhouse associated with a campylobacter outbreak in 1984 caused by this serotype. the serotype persisted on the farm for at least 18 months after the outbreak; its prevalence in the human population served by the farm remained high until it disappeared from the farm in 1986. the possible sources and routes of t ... | 1993 | 8476300 |
| incidence and control of campylobacter in foods. | campylobacter jejuni is known as the most common human enteropathogen in developed countries. the epidemiology of the organism has now been adequately determined and indicates that campylobacteriosis is a zoonotic disease. pets, water, and contaminated foods are the main sources of sporadic infections in humans, and no single animal food source can be excluded as a potential vehicle for infection of humans. the available information indicates that 50 to 70% of cases of enteritis in man are assoc ... | 1993 | 8484918 |
| heat-labile and heat-stable haemolysins of campylobacter jejuni. | during studies on the virulence mechanisms of campylobacter jejuni clinical isolates it became apparent that some strains produced one or more haemolysins and some did not. there was no great difference between group c (cholera-like) strains and group d (dysentery-like) strains. the protein haemolysin(s) showed a spectrum of activity against erythrocytes from different animals; with maximum activity against rabbit and minimal activity against chicken erythrocytes. the results suggested a two-sta ... | 1993 | 8499897 |
| role of campylobacter jejuni flagella as colonization factors for three-day-old chicks: analysis with flagellar mutants. | campylobacter jejuni, an important cause of human gastrointestinal infection, is a major food-borne pathogen in the united states and worldwide. since poultry becomes colonized and/or contaminated during the early stages of production and is a major food-borne source for this organism, we studied the role of c. jejuni flagella on the ability of the bacterium to colonize the chicken gastrointestinal tract. three-day-old chicks were orally challenged with a motile wild-type strain of c. jejuni in9 ... | 1993 | 8517729 |
| the colonization of broiler chickens with campylobacter jejuni: some epidemiological investigations. | between june 1990 and july 1991, broiler chickens from 49 flocks from 23 farms were examined for the carriage of campylobacter jejuni at slaughter. thirty-seven flocks (76%) were campylobacter-positive. prevalence of campylobacter-colonization was not associated with any of a variety of factors such as water source and broiler house floor structure. there was also no apparent seasonal variation in carriage. investigations on one farm indicated that dipping boots in disinfectant before workers en ... | 1993 | 8519325 |
| efficacy of a lactic acid/sodium benzoate wash solution in reducing bacterial contamination of raw chicken. | raw chicken wings inoculated with salmonella, campylobacter jejuni, listeria monocytogenes, staphylococcus aureus, or escherichia coli o157:h7 were washed in water (control) or a solution of a 0.5% lactic acid/0.05% sodium benzoate (lb) (ph 2.64) for 30 min. viable cells of pathogenic bacteria and naturally occurring psychrotrophic bacteria on wings were enumerated after 0, 2, 4, 6, and 8 days of storage at 4 degrees c. lower populations of pathogenic and psychrotrophic bacteria were detected on ... | 1995 | 8527331 |
| effect of changes in processing to improve hygiene control on contamination of poultry carcasses with campylobacter. | examination of neck skin and caecal samples taken at a commercial processing plant from 15 randomly chosen poultry flocks showed that all flocks were contaminated initially with thermophilic campylobacter spp., even in the apparent absence of caecal carriage. during processing, numbers of campylobacter on skin samples were reduced by between 10 and 1000-fold. to improve hygiene control generally, chlorinated-water sprays were used to limit microbial contamination on equipment and working surface ... | 1995 | 8557081 |
| evaluation of a pcr based assay for specific detection of campylobacter jejuni in chicken washes. | an assay for campylobacter jejuni based on the polymerase chain reaction was developed in our laboratory and shown to be a sensitive and specific method to identify this bacterium in pure culture. this assay was evaluated as a method to rapidly detect c. jejuni attached to chicken carcasses. chicken carcasses were sampled for pcr using three methods including pre-enrichment of the washes, direct plating of the washes and differential centrifugation of the washes prior to testing. it was found th ... | 1995 | 8569769 |
| identification and characterization of an immunogenic outer membrane protein of campylobacter jejuni. | we cloned and expressed in escherichia coli a gene encoding an 18-kda outer membrane protein (omp18) from campylobacter jejuni atcc 29428. the nucleotide sequence of the gene encoding omp18 was determined, and an open reading frame of 165 amino acids was revealed. the amino acid sequence had the typical features of a leader sequence and a signal peptidase ii cleavage site at the n-terminal part of omp18. moreover, the sequence had a high degree of similarity to the peptidoglycan-associated outer ... | 1995 | 8576327 |
| in ovo oral vaccination with campylobacter jejuni establishes early development of intestinal immunity in chickens. | 1. chick embryos were orally immunised at day 16 of incubation by injection of heat-killed campylobacter jejuni organisms into the amniotic fluid. the response to vaccination was observed at 5 d after hatching or, in some birds which received a postnatal oral booster vaccination, at 7 d after hatching, and the response was observed at 14 d of age. 2. the titres of antibody in serum, bile and intestinal scrapings, the distribution of immunoglobulin-containing cells in the spleen, duodenum and ile ... | 1995 | 8590089 |
| restriction fragment length polymorphism of polymerase chain reaction products applied to the differentiation of poultry campylobacters for epidemiological investigations. | a technique for subtyping campylobacter jejuni isolates has been developed by using the restriction fragment length polymorphism (rflp) of polymerase chain reaction (pcr) products of the flaa and flab genes. the technique was validated by using strains representing 28 serotypes of c jejuni and it may also be applied to c coli. from these strains 12 distinct rflp profiles were observed but there was no direct relationship between the rflp profile and the serotype. one hundred and thirty-five camp ... | 1996 | 8685540 |
| in vitro studies of campylobacter jejuni/coli strains from hens and humans regarding adherence, invasiveness, and toxigenicity. | campylobacter jejuni/coli strains from hens and humans were compared for their ability to adhere to and invade hep-2-cells and for toxigenicity to cho-cells. in both hen and human strains, invasiveness was higher among non-toxigenic strains than among toxigenic ones. the frequency of adherence, invasiveness, and toxigenicity was the same in hen and human strains. | 1995 | 8719204 |
| a genetic hybrid of the campylobacter jejuni flaa gene with lt-b of escherichia coli and assessment of the efficacy of the hybrid protein as an oral chicken vaccine. | the objectives of this study were to produce campylobacter jejuni flagellin fused to the b-subunit of the labile toxin (lt-b) of escherichia coli and to assess the efficacy of the hybrid protein as a chicken vaccine. part of the flaa gene (780 base pairs) was cloned in plasmid pbeb downstream and in frame with the lt-b to allow expression of a hybrid protein. transformed e. coli chi 6097 expressed the hybrid protein (43 kdaltons) in inclusion bodies at mid log phase. the inclusion bodies were is ... | 1995 | 8719215 |
| ciprofloxacin resistant campylobacter spp. in humans: an epidemiological and laboratory study. | from the end of april 1991 until the end of 1991, 2209 isolates of campylobacter spp. have been collected in plymouth phl of which 91 (4.1%) were resistant to ciprofloxacin. none of the 91 patients involved had taken a quinolone, but 30/91 (33%) had travelled abroad (16 to the iberian peninsula) in the three months preceding isolation of the ciprofloxacin-resistant campylobacter spp. in the case-control study 12/15 (80%) of the cases had recently consumed poultry as had 20/24 (83%) of controls w ... | 1996 | 8722540 |
| antimicrobial activity of chicken and turkey heterophil peptides chp1, chp2, thp1, and thp3. | four avian heterophil antimicrobial cationic peptides (chicken heterophil peptides 1 and 2, and turkey heterophil peptides 1 and 3) were evaluated for in vitro microbicidal activity against selected avian pathogens and human pathogens which are harbored by birds. at concentrations of 16-2 micrograms/ml, all four avian peptides effected a greater than 90% reduction in the survival of candida albicans, salmonella enteriditis, and campylobacter jejuni. none of the peptides, including the known anti ... | 1995 | 8748545 |
| increased colonization potential of campylobacter jejuni strain 81116 after passage through chickens and its implication on the rate of transmission within flocks. | the mechanisms by which campylobacter jejuni rapidly spreads through large broiler flocks are unknown. recent evidence suggests that the minimum infective oral dose for 100% caecal colonization is about 10(4) cfu, which, with such a 'fragile' organism, may limit transmissibility. however, the colonization potential of laboratory-adapted strains may be anomalous compared with fresh isolates or those passaged in vivo. the colonization potential of two chicken and one human c. jejuni isolates, whic ... | 1996 | 8760971 |
| experimental hepatitis induced by campylobacter jejuni infection in japanese quail (coturnix coturnix japonica). | to establish an experimental model for vibrionic hepatitis caused by campylobacter jejuni, japanese quails (coturnix coturnix japonica) were inoculated with c. jejuni strains isolated from chicken hepatitis (bl107) and human diarrhea (hp5113). necrotic liver lesions were formed by intra-pancreaticoduodenal vein injection by which the bacteria reached the liver directly via the portal vein, but not by intra-gastric infection. these liver lesions were observed from day 1 to 7 after the infection. ... | 1996 | 8777226 |
| experimental campylobacter jejuni infection in the chicken: an animal model of axonal guillain-barré syndrome. | to develop and characterise an animal model of paralytic neuropathy after campylobacter jejuni infection. campylobacter infection precedes development of many cases of guillain-barré syndrome and is particularly associated with cases having prominent axonal degeneration. understanding the pathogenesis of guillain-barré syndrome after c jejuni infection has been slowed by the lack of animal models. | 1996 | 8795599 |
| immunisation of chickens to reduce intestinal colonisation with campylobacter jejuni. | 1. systemic and intestinal antibody titres were measured in chickens following subcutaneous, intraperitoneal (i.p.), oral (p.o.) and combined i.p./p.o. administration of antigen, in soluble, emulsified or microparticulate form. antigens tested included keyhole limpet haemocyanin (klh), killed campylobacter jejuni whole cells and purified campylobacter flagellin protein. 2. the effect of immunisation with purified flagellin protein or with killed c. jejuni whole cells in reducing intestinal colon ... | 1996 | 8894221 |
| development of a rapid and specific colony-lift immunoassay for detection and enumeration of campylobacter jejuni, c. coli, and c. lari. | contamination of retail poultry by campylobacter spp. is a significant source of human diarrheal disease. we have developed a colony-lift immunoassay (cli) for the detection of campylobacter jejuni, c. coli, and c. lari isolated from such sources and grown on selective agar medium or on filter membranes. this technique has been successfully utilized to quantify campylobacter colonies within 18 to 28 h after sampling. hydrophobic, high-protein-binding membranes were prewet with methanol and used ... | 1996 | 8914757 |
| the magnetic immuno-polymerase chain reaction assay for the detection of campylobacter in milk and poultry. | a rapid and sensitive technique, based on the magnetic immuno-polymerase chain reaction assay (mipa), was developed for the detection of campylobacter jejuni in milk and chicken products. target bacteria are captured from the food sample by magnetic particles coated with a specific antibody and the bound bacteria then lysed and subjected to pcr. the mipa could detect 420 cfu g-1 of chicken after 18 h, 42 cfu g-1 after 24 h, and 4.2 cfu g-1 after 36 h enrichment. for artificially contaminated mil ... | 1996 | 8934788 |
| microbial ecology of campylobacter jejuni in a united kingdom chicken supply chain: intermittent common source, vertical transmission, and amplification by flock propagation. | a study of campylobacter jejuni on a broiler chicken farm between 1989 and 1994 gave an estimated isolation rate of 27% (3,304 of 12,233) from a 0.9% sample of 1.44 million broiler chickens from six to eight sheds over 32 consecutive rearing flocks comprising 251 broiler shed flocks. during the study, c. jejuni was found in 35.5% of the 251 shed flocks but only 9.2% (23 of 251) had campylobacter isolates in successive flocks, with 9 of those 23 sheds having the same serotype between consecutive ... | 1996 | 8953731 |
| experimental studies on the infectivity of non-culturable forms of campylobacter spp. in chicks and mice. | the significance of non-culturable forms of campylobacter spp., especially with regard to the epidemiology of this organism in poultry flocks, was explored. two different experiments were conducted to produce non-culturable campylobacter spp. and test their ability to colonize the animal gut. in the first experiment a mixture of 28 different strains of campylobacter spp. from various sources was inoculated in both sterilized surface water and potassium phosphate buffer and stored at 4 degrees c. ... | 1996 | 8972670 |
| outer membrane characteristics of campylobacter jejuni grown in chickens. | a type of in vivo phenotype of campylobacter jejuni was obtained by maintaining bacteria in the peritoneal cavities of chickens for one week. these bacteria, which had not been subcultured on laboratory media, were used to prepare outer membranes for comparison with c. jejuni grown in vitro. flagella with subunits of 65 kda and a single porin with a protein subunit of 49 kda were expressed constitutively; however, outer membrane proteins of 55, 35 and 20 kda, and intermediate-chain lipopolysacch ... | 1996 | 8978102 |
| isolation of paramyxovirus serotype 7 from ostriches (struthio camelus). | paramyxovirus serotype 7 (pmv-7) was isolated from pooled intestinal contents of two 5-month-old ostriches (struthio camelus). the pathogenicity of the virus was comparable with lentogenic strains of newcastle disease virus (pmv-1) in chicken and chicken embryo pathogenicity tests. the relationship of the virus to the observed pathology of proliferative nonsuppurative enteritis is unknown; the campylobacter jejuni isolated was presumably the primary pathogen. to our knowledge, this is the first ... | 1996 | 8980831 |
| detection of campylobacter jejuni and camp. coli in chicken faecal samples by pcr. | pcr primers were selected from the flagellin gene sequences flaa and flab of campylobacter coli to amplify dna from camp. jejuni and camp. coli. when the pcr products were analysed by hybridization to an internal probe immobilized in microtitre wells, positive reactions were observed only for strains of camp. jejuni and camp. coli. the assay was used to analyse 31 chicken faecal samples. full correspondence was found between the pcr assay conducted on the enriched cultures and the standard cultu ... | 1996 | 8987720 |
| application of the mast resistotyping scheme to campylobacter jejuni and c. coli. | the mast resistotyping scheme was assessed with 228 strains of campylobacter jejuni and c. coli from enteric infections in man and from a diverse selection of other sources (livestock, chickens and river water). most (153 of 158) c. jejuni examined were of the three most common penner (heat stable, hs) serotypes, hs1, hs2 and hs4 complex. fourteen resistotypes were identified in the 158 strains of c. jejuni and 16 in the 70 isolates of c. coli. the predominant codes were 00 (44% of c. jejuni; 33 ... | 1997 | 9003743 |
| rapid identification of campylobacter jejuni strains by polymerase chain reaction & their restriction fragment length polymorphism analysis. | a polymerase chain reaction (pcr) technique was developed for specific identification of c. jejuni. a primer pair of a conserved region of flagellin a (fla a) gene identified all 15 strains of c. jejuni isolated from human faeces. none of the control strains like helicobacter pylori, vibrio cholerae escherichia coli and salmonella typhimurium except c. coli exhibited any amplified product by pcr. a predicted 450 bp could also be amplified from 4 chicken caecal contents positive for c. jejuni-c c ... | 1997 | 9029829 |
| direct detection of campylobacter jejuni in chicken cecal contents by pcr. | campylobacter jejuni in chicken feces was detected by pcr and southern blot hybridization (sbh). the detection limits of c. jejuni in chicken feces were 34,000 cells by pcr and 340 cells by sbh. some cecal contents of chickens up to 3 weeks old were c. jejuni positive by sbh whereas all of them were negative by pcr. two of 51 cecal contents of 18-day-old chicken embryos were c. jejuni positive by pcr and sbh; but, c. jejuni were not isolated from the samples by conventional culture with selectiv ... | 1997 | 9035088 |
| campylobacter jejuni seasonal recovery observations of retail market broilers. | this study investigated possible seasonal trends in the campylobacter jejuni carrier state of market broilers. in this study, broiler carcasses, 15 each of two major companies, were obtained from a local supermarket each month for an entire year to evaluate the presence of c. jejuni on the carcasses. direct plating and the whole carcass rinse procedure were used for c. jejuni detection. resuscitation of damaged cells and preenrichment of low numbers of micoorganisms were accomplished by hunt's p ... | 1997 | 9057212 |