| cell lineage analysis in ascidian embryos by intracellular injection of a tracer enzyme. iii. up to the tissue restricted stage. | cell lineages during embryogenesis of the ascidian halocynthia roretzi were analyzed up until the stage where each blastomere was fated to be only a single tissue type (i.e., the tissue restricted stage) by intracellular injection of horseradish peroxidase using the iontophoretic injection method. initially, the developmental fates of all blastomeres of the 64-cell stage embryo were examined, and thereafter, only the fates of daughter blastomeres of those blastomeres that were not tissue restric ... | 1987 | 3582738 |
| serum tsh measurements by a sensitive enzyme immunoassay discriminate euthyroid from hyperthyroid subjects and avoid the need for trh test during suppressive therapy with l-thyroxine. | serum tsh was determined photometrically by a recently developed enzyme immunoassay (eia) based on the use of a monoclonal antihuman tsh-beta antibody and a polyclonal antitsh antibody coupled to horseradish peroxidase. the results obtained in patients with various thyroid disorders and in normal controls were compared with those achieved by conventional double antibody radioimmunoassay (ria). in normal subjects, serum tsh was detectable in all cases by eia (values ranging from 0.27 to 5.1 mu/l) ... | 1987 | 3115625 |
| structure and properties of enzyme graft copolymers: effects of using dissolved agarose on horseradish peroxidase immobilization. | the immobilization of horseradish peroxidase (hrp) onto dissolved agarose by a photochemically initiated graft copolymerization reaction, carried out at room temperature, was studied. enzyme immobilization parameters such as the catalyst (fecl3) and the enzyme concentration were considered. using hexhydro-1,3,5-triacryloyl-s-triazine (htst) as vinyl monomer, the agarose/htst ratio was the main reaction parameter controlling the copolymer characteristics. by increasing the polymer content of the ... | 1987 | 3111494 |
| the peripheral distribution and central projections of the sensory rami of the facial nerve in goldfish, carassius auratus. | taste buds in goldfish and other cyprinids are found not only within the oropharyngeal cavity but also scattered over the external body surface. the external taste buds are innervated by branches of the facial nerve that terminate centrally in an enlargement of the medulla termed the facial lobe. the peripheral distribution and areas of innervation of the rami of the facial sensory nerve were determined by using a modification of the sihler technique and by examination of a bodian-stained head s ... | 1987 | 3584562 |
| distribution of motoneurons involved in the prey-catching behavior in the japanese toad, bufo japonicus. | coordinated activities of several muscles in the head region underlie the prey-catching behavior of anuran amphibians. as a step in elucidating the neural mechanisms generating these activity patterns in the japanese toad, we labelled the motoneurons innervating 8 behaviorally relevant muscles using intramuscular (i.m.) injection technique of horseradish peroxidase (hrp), and examined their localization within the motor nuclei whose boundaries were determined by hrp application to the nerve trun ... | 1987 | 3594249 |
| quantitative studies of hydroperoxide reduction by prostaglandin h synthase. reducing substrate specificity and the relationship of peroxidase to cyclooxygenase activities. | the peroxidase activity of prostaglandin h (pgh) synthase catalyzes reduction of 5-phenyl-4-pentenyl hydroperoxide to 5-phenyl-4-pentenyl alcohol with a turnover number of approximately 8000 mol of 5-phenyl-4-pentenyl hydroperoxide/mol of enzyme/min. the kinetics and products of reaction establish pgh synthase as a classical heme peroxidase with catalytic efficiency similar to horseradish peroxidase. this suggests that the protein of pgh synthase evolved to facilitate peroxide heterolysis by the ... | 1987 | 3106353 |
| long-term storage of peroxidase-labelled immunoglobulins for use in enzyme immunoassay. | optimal conditions for long-term storage of peroxidase-labelled immunoglobulins for use in enzyme immunoassays have been investigated with particular emphasis on the preservation of the immunological and enzyme activity. immunoglobulin g preparations from different animal species were purified according to conventional methods and labelled with horseradish peroxidase. conjugates were stored at different temperatures either in liquid form with 50% glycerol, after lyophilization or as ammonium sul ... | 1987 | 3033084 |
| the utilization of biotin-antibiotin interaction for the detection of antibody to hepatitis b surface antigen (anti-hbs). | a biotin-antibiotin solid-phase enzyme-linked immunoassay for the detection and quantitation of the antibody to hepatitis b surface antigen (anti-hbs) is described. the assay utilizes hepatitis b surface antigen as a solid-phase 'capture' reagent and a mixture of biotinylated hbsag and antibiotin-conjugated horseradish peroxidase as a detector reagent. the assay was compared to a commercial enzyme immunoassay (ausab eia) which used the biotin-avidin system for anti-hbs detection. the two assays ... | 1987 | 3611288 |
| a biotin/avidin solid-phase sandwich enzyme immunoassay for the antibody to hepatitis b surface antigen (anti-hbs). | a biotin/avidin solid-phase enzyme immunoassay for the detection and quantitation of the antibody to hepatitis b surface antigen (anti-hbs) is described. the assay utilizes hepatitis b surface antigen (hbsag) as a solid-phase 'capture' reagent and a mixture of biotinylated hbsag and avidin-conjugated horseradish peroxidase as a probe 'detector' reagent. the assay was compared to a commercial radioimmune assay for anti-hbs detection. the two assays were found to measure the same molecules and to ... | 1987 | 3611287 |
| classes of neurons in relation to the laminar organization of the lateral geniculate nucleus in the tree shrew, tupaia belangeri. | we used the rapid golgi and horseradish peroxidase (hrp) techniques to study the dendritic spread of relay neurons in functionally distinct laminae of the tree shrew dorsal lateral geniculate nucleus (lgnd). on the basis of their dendritic spread in relation to laminar and interlaminar zones, we describe three classes of relay neurons. unilaminar neurons with multipolar radiate, bitufted, and intermediate types of dendrites. dendrites of these neurons are confined to one lamina only, but also ca ... | 1987 | 3584555 |
| [induced regression of established buccal carcinomas and suppression of dysplastic changes in the oral mucosa using oxygen radical generating systems: an objective study of glucose oxidase, horseradish peroxidase and hyperbaric oxygen]. | | 2006 | 3269143 |
| sorting of endocytosed transferrin and asialoglycoprotein occurs immediately after internalization in hepg2 cells. | after receptor-mediated uptake, asialoglycoproteins are routed to lysosomes, while transferrin is returned to the medium as apotransferrin. this sorting process was analyzed using 3,3'-diaminobenzidine (dab) cytochemistry, followed by percoll density gradient cell fractionation. a conjugate of asialoorosomucoid (asor) and horseradish peroxidase (hrp) was used as a ligand for the asialoglycoprotein receptor. cells were incubated at 0 degree c in the presence of both 131i-transferrin and 125i-asor ... | 1987 | 3032986 |
| a comparison by magnetic circular dichroism of compound x and compound ii of horseradish peroxidase. | the chlorite product of horseradish peroxidase, compound x, is shown by magnetic circular dichroism (mcd) spectroscopy in the temperature range 1.6-50 k to have a very similar haem structure to compound ii under the same conditions (ph 10.7). both are concluded to contain the fe(iv) = 0 group. the mcd spectrum also detects an unusual species, absorbing at wavelengths between 600 and 750 nm, that has magnetic properties different from those of the ferryl haem group. it is suggested that this is a ... | 1987 | 3569529 |
| retinopretectal and accessory optic projections of normal mice and the okn-defective mutant mice beige, beige-j, and pearl. | retinal projections to the pretectal and terminal accessory optic nuclei were studied in normal wild-type mice and mutant mice with abnormal optokinetic nystagmus (okn, mangini, vanable, williams, and pinto: j. comp. neurol. 241:191-209, '85). the mutants used were pearl, which exhibits an inverted okn in response to stimulation of only the temporal retina, and beige and beige-j, which show inverted okn in response to stimulation of only the temporal retina and, in addition, exhibit eye movement ... | 1987 | 3584547 |
| percentage of intrinsic and relay cells in a thalamic nucleus projecting to general cortex in reptiles, caiman crocodilus. | small injections of horseradish peroxidase (hrp) restricted solely to the general cortex in reptiles, caiman crocodilus, resulted in retrogradely labeled cells located bilaterally in the nucleus dorsolateralis anterior of the thalamus. larger cortical injections of hrp labeled greater numbers of nucleus dorsolateralis anterior cells. hrp injections designed to label as much cortex as possible resulted in very few unlabeled ipsilateral neurons in the nucleus dorsolateralis anterior. the percentag ... | 1987 | 3580863 |
| two types of conformers with distinct fe-c-o configuration in the ferrous co complex of horseradish peroxidase. resonance raman and infarared spectroscopic studies with native and deuteroheme-substituted enzymes. | the presence of at least two types of conformers in the ferrous co complex of horseradish peroxidase has been demonstrated with the use of native and deuteroheme-substituted enzymes. type i conformers, predominant in acidic ph, exhibited both an fe-co stretching and an fe-c-o bending raman line together with an infrared c-o stretch band below 1920 em-1. on the other hand, type ii conformers, dominant species in alkaline ph, showed only an fe-co stretching raman line with the c-o stretch above 19 ... | 1987 | 3558355 |
| lineage analysis of transplanted individual cells in embryos of drosophila melanogaster : iv. commitment and proliferative capabilities of mesodermal cells. | we describe the results of cell transplantation experiments performed to investigate mesodermal lineages in drosophila melanogaster, particularly the lineages of the somatic muscles, the visceral muscles and the fat body. cells to be transplanted were labelled by injecting a mixture of horseradish peroxidase (hrp) and fluorescein-dextran (fitc) in wild-type embryos at the syncytial blastoderm stage. for transplantation cells were removed from the ventral furrow, 8-12 min after the start of gastr ... | 1987 | 28305697 |
| a computer-assisted study of quantitative morphological features of c type horizontal cells in retina. | biphasic c type horizontal cells of the retina of crucian carp were intracellularly stained with horseradish peroxidase (hrp) and quantitatively studied with computer-aided three-dimensional reconstruction techniques. several parameters characterizing their morphology were determined and compared among different subtypes of horizontal cells. the results show that both cell body area and horizontal extension are proper parameters by which different subtypes of biphasic horizontal cells can be cle ... | 1987 | 3659894 |
| a retinal whole-mount method useful in detecting retrogradely-labeled ganglion cells. | the present report describes a simple retinal whole-mount method that is compatible with horseradish peroxidase histochemistry. the retina is removed unfixed, flattened and fixed. histochemical procedures are carried out with the retina free-floating, prior to affixing it to the slide with formaldehyde vapor. the method consistently yields whole-mounts free of pigment epithelium and firmly affixed to the slide, with good preservation of retinal morphology and surface topography. | 1987 | 3607525 |
| the postnatal development of the junctional complexes of hamster sertoli cells as revealed by hrp and freeze-fracture. | the development of the sertoli cells occluding junctions was investigated in testes from 5-, 10-, 15-, 20- and 30-day-old hamsters after intravascular administration of horseradish peroxidase (hrp) and by freeze-fracture. up to the 15th day, hrp could be detected in the interstitial spaces and the central area of testicular cords. on day 20, the tracer reached the lumen of some tubules but in others it was retained at the periphery of the seminiferous epithelium, thus suggesting an asynchronous ... | 1987 | 3599128 |
| morphometric analysis of neuronal soma size within the motor nucleus of a transplanted murine skeletal muscle. | since the number of motor neurons supplying a muscle graft is reduced, the peripheral field for the surviving motor neurons would be enlarged. this possible change in motor unit size may result in morphologic changes in the size of motor neurons within the motor neuron pool of the graft. the extensor digitorum longus (edl) muscle from 19 transplanted and 17 age-matched normal 129 rej female mice were injected with horseradish peroxidase in order to examine the cell size distributions of the moto ... | 1987 | 3592267 |
| interendothelial junctional changes underlie the developmental 'tightening' of the blood-brain barrier. | the structural basis for the decline in non-specific permeability (tightening) of the blood-brain barrier (bbb) during development was investigated in fetal, newborn and weanling mice cerebral hemispheres. permeability was assessed by measuring the peroxidase activity in the brain 4 h after i.p. injection of horseradish peroxidase--a commonly used vascular tracer that does not cross the intact bbb. peroxidase activity in brain declined by about two-thirds between birth and weaning, despite incre ... | 1987 | 3567665 |
| distribution of visual callosal projection neurons in hamsters subjected to transection of the optic radiations on the day of birth. | the optic radiations of hamsters were transected on the day of birth and visual callosal projections in these animals were traced using retrograde transport of either horseradish peroxidase (hrp) or the fluorescent tracers true blue (tb) or diamidino yellow (dy) when the animals reached maturity (greater than 45 days of age). in the hemisphere ipsilateral to the neonatal lesion, the distribution of callosal cells was markedly altered. these neurons were almost completely restricted to a continuo ... | 1987 | 3567664 |
| control of membrane permeability in animal cells by divalent cations. | the permeability of several cell lines, including hela, l929, 3t6 and 3t3, to various compounds is affected by the concentration of divalent cations in the culture medium. in the absence of mg2+ ions but with 4-8 mm cacl2 in the medium, hela and l929 cells become permeabilized, as measured by the entry of the aminoglycoside antibiotic hygromycin b. however, 3t3 and 3t6 cells become much more permeable when calcium and magnesium are both absent from the medium. addition of mg2+ above 2 mm abolish ... | 1987 | 3556432 |
| blood-brain barrier permeability during cuprizone-induced demyelination. implications for the pathogenesis of immune-mediated demyelinating diseases. | blood vessels in the superior cerebellar peduncles were studied in cuprizone-fed mice for leakage of proteins into the parenchyma. the status of the blood-brain barrier was determined at various stages of demyelination both by tracer methods using horseradish peroxidase and immunochemically using antisera to extravasated serum proteins and was compared to three positive and negative control conditions. the results showed no evidence of significant protein leakage into the subendothelial basement ... | 1987 | 3553434 |
| neuronal organization underlying visually elicited prey orienting in the frog--ii. anatomical studies on the laterality of central projections. | a complete transverse hemisection of the neuraxis just caudal to the optic tectum in the frog, rana pipiens, results in a failure to orient toward stimuli in one visual hemifield [kostyk and grobstein (1986) neuroscience 21, 41-55]. the extent of the deficit area implies disturbances in the outputs triggered by both tectal lobes. in this paper we report studies aimed at determining more precisely what damage is involved in producing the hemisection deficit, with the broader objective of identify ... | 1987 | 3496553 |
| spin trapping artifacts in dmso. | spin-trapping experiments in alkaline aqueous dimethyl sulfoxide (dmso) solution using sodium 3,5-dibromo-4-nitrosobenzenesulfonate (dbnbs) yielded a strong signal of the sulfur trioxide anion radical adduct. this radical adduct is identical to that obtained by the oxidation of sulfite with horseradish peroxidase/hydrogen peroxide and subsequent spin trapping with dbnbs. this radical adduct is very stable, and satellite peaks of the natural abundance 13c and 33s could be obtained. apparently, un ... | 1987 | 3032190 |
| proton nuclear magnetic resonance studies on the iodide binding by horseradish peroxidase. | binding of an iodide ion to horseradish peroxidase was studied by following the hyperfine-shifted proton nuclear magnetic resonance signals of the enzyme. for the enzyme in an iodide-free solution, the spectra of hyperfine-shifted methyl region were only slightly affected by varying ph. in the presence of iodide (200 mm), however, both chemical shifts and line widths of the heme peripheral 1- and 8-methyl proton signals were markedly affected by the ph change from 7 to 4 and broadened at ph 4. f ... | 1987 | 3558404 |
| recombination of carbon monoxide to ferrous horseradish peroxidase types a and c. | the recombination of carbon monoxide to isoenzymes a2 and c of horseradish peroxidase (hrp) was studied as a function of temperature (2 to 320 k) and ph (5 to 8.3) with flash photolysis and infrared difference absorption. at low temperatures three geminate recombination processes are observed. one of these internal processes, denoted by i*, is exponential in time with a rate coefficient that deviates strongly from an arrhenius behavior below 100 k, implying phonon-assisted tunneling. the two oth ... | 1987 | 3612808 |
| origin of the climbing fibers activated by glossopharyngeal nerve stimulation in the frog. | the origin of climbing fibers activated by electrical stimulation of the frog's glossopharyngeal (ixth) nerve was investigated using histological and electrophysiological technique. at the molecular layer near the purkinje cell layer, where the maximum negative cerebellar field potential could be recorded following electrical stimulation of the ixth nerve, horseradish peroxidase (hrp) was iontophoretically injected through the tip of the recording micropipette. the hrp labeled cells were seen in ... | 1987 | 3494964 |
| electron paramagnetic resonance and spectrophotometric studies of the peroxide compounds of manganese-substituted horseradish peroxidase, cytochrome-c peroxidase and manganese-porphyrin model complexes. | peroxide compounds of manganese protoporphyrin ix and its complexes with apo-horseradish peroxidase and apocytochrome-c peroxidase were characterized by electronic absorption and electron paramagnetic resonance spectroscopies. an intermediate formed upon titration of mn(iii)-horseradish peroxidase with hydrogen peroxide exhibited a new electron paramagnetic resonance absorption at g = 5.23 with a definite six-lined 55mn hyperfine (amn = 8.2 mt). neither a porphyrin pi-cation radical nor any othe ... | 1987 | 3030431 |
| transient state kinetics of the reactions of isobutyraldehyde with compounds i and ii of horseradish peroxidase. | elementary reactions have been studied quantitatively in the complex overall process catalyzed by horseradish peroxidase whereby isobutyraldehyde and molecular oxygen react to form triplet state acetone and formic acid. the rate constant for the reaction of the enol form of isobutyraldehyde with compound i of peroxidase is (8 +/- 1) x 10(6) m-1 s-1 and with compound ii (1.3 +/- 0.3) x 10(6) m-1 s-1. neither the enolate anion nor the keto form is reactive. the reactivity of enols with peroxidase ... | 1987 | 3818656 |
| organization of intracortical and commissural connections in somatosensory cortical areas i and ii in the raccoon. | the organization of intracortical and callosal projecting cell bodies was examined in somatosensory representation areas i (si) and ii (sii) of the raccoon by use of horseradish peroxidase (hrp) or horseradish peroxidase-wheat germ agglutin (hrp-wha). hrp and hrp-wha were injected into commissurally and noncommissurally connected subdivisions of si and sii. injection sites in sii were identified electrophysiologically. results were obtained from transverse sections in which the hrp was visualize ... | 1987 | 3558894 |
| the mechanism of potentiation of horseradish peroxidase-catalysed oxidation of nadph by porphyrins. | several porphyrins, including hpd (haematoporphyrin derivative), potentiate the oxidation of nadph by horseradish peroxidase/h2o2. to elucidate the mechanism of potentiation, the following observations are relevant. during peroxidase-catalysed nadph oxidation, o2-.(superoxide radical) is generated, as judged from superoxide dismutase-inhibitable cytochrome c reduction. this generation of o2-. is suppressed by hpd. peroxidase-catalysed nadph oxidation is stimulated by superoxide dismutase and by ... | 1987 | 3036094 |
| the mechanism of oxyperoxidase formation from ferryl peroxidase and hydrogen peroxide. | formation of oxyperoxidase from the reaction of ferryl horseradish peroxidase with h2o2 is inhibited by a small amount of tetranitromethane (tnm), a powerful scavenger of superoxide anion radical. the inhibition by tnm, however, does not exceed 35% as the tnm concentration is increased above 5 microm. the stoichiometry of the reaction in the presence of tnm suggests the following equation for tnm-sensitive formation of oxyperoxidase. ferryl peroxidase + h2o2----(ferric peroxidase + o2- + h+)---- ... | 1987 | 3029087 |
| corticospinal projections originate from the arcuate premotor area. | we investigated the patterns of corticospinal projection to different segments of the cervical spinal cord using retrograde transport of wheat germ agglutinin conjugated to horseradish peroxidase. we confirmed prior suggestions that the arcuate premotor area (apa) has a significant projection to the spinal cord. in addition, we observed that the corticospinal projection from the apa terminates in upper, but not lower segments of the cervical spinal cord. furthermore, the region of the apa which ... | 1987 | 3032334 |
| morphology and distribution of the projection neurons in the cerebellum in a teleost, sebastiscus marmoratus. | cerebellar efferent neurons in a teleost, sebastiscus marmoratus, were studied by means of the horseradish peroxidase (hrp) tracing and golgi impregnation methods. the cerebellar efferents or eurydendroid neurons are classified into two types (a and b) on the basis of the morphology of the dendrites, intracerebellar distribution, and other afferent connections. type a neurons form a cell cluster in the lobus caudalis and have one thick primary dendrite from which several branches with sparse but ... | 1987 | 3558892 |
| kinetics of the oxidation of ascorbic acid, ferrocyanide and p-phenolsulfonic acid by chloroperoxidase compounds i and ii. | for the first time elementary reactions involving chloroperoxidase compounds i and ii have been investigated. a multi-mixing stopped-flow apparatus was used to study the kinetics of the reactions of compounds i and ii with ascorbic acid, ferrocyanide and p-phenolsulfonic acid. the second-order rate constants of the reactions of both compounds with all three substrates were determined between ph 3 and ph 7. in all cases the rate constants decrease with increasing ph. the reactions of p-phenolsulf ... | 1987 | 3816791 |
| protein control of prosthetic heme reactivity. reaction of substrates with the heme edge of horseradish peroxidase. | incubation of horseradish peroxidase with phenylhydrazine and h2o2 markedly depresses the catalytic activity and the intensity, but not position, of the soret band. approximately 11-13 mol of phenylhydrazine and 25 mol of h2o2 are required per mol of enzyme to minimize the chromophore intensity. the enzyme retains some activity after such treatment, but this activity is eliminated if the enzyme is isolated and reincubated with phenylhydrazine. the prosthetic heme of the enzyme does not react wit ... | 1987 | 3805041 |
| the fate of persisting thoracic neurons during metamorphosis of the meal beetle tenebrio molitor (insecta: coleoptera). | a set of motor neurons and interneurons in the thoracic nervous system of the meal beetle tenebrio molitor l. is described that persist during metamorphosis. the motor neurons under discussion innervate the thoracic ventral longitudinal muscles and were identified by retrograde transport of intramuscularly injected horseradish peroxidase. persisting motor neurons exhibit a complex repetitive pattern that changes only slightly during development. additionally, the characterization of serotonin-im ... | 1987 | 28305463 |
| terminations of individual optic tract fibers in the lateral geniculate nuclei of galago crassicaudatus and tupaia belangeri. | the morphology and laminar distribution of individual optic fibers projecting to the lateral geniculate nucleus (gl) of galago and tupaia were studied following iontophoretic injections of horseradish peroxidase (hrp) into the optic tract. in galago the gl is composed of three functionally matched pairs of layers, each characterized by cells of a given size, one large, one medium-sized, and one small. the results show that there is a close correspondence between the size of the afferent fibers a ... | 1987 | 3819039 |
| fates of the blastomeres of the 16-cell stage xenopus embryo. | the fate of each of the blastomeres in the 16-cell stage xenopus embryo which had been carefully selected for stereotypic cleavages was determined by intracellularly marking a single blastomere with horseradish peroxidase and identifying the labeled progeny in the tailbud embryo by histochemistry. each blastomere populated all three primary germ layers. the progeny of each blastomere were distributed characteristically both in phenotype and in location. for example, most organs were populated by ... | 1987 | 3803718 |
| reformation of the pattern of neuromuscular connections in the regenerated axolotl hindlimb. | retrograde neuronal tracing with horseradish peroxidase (hrp) was used to determine the position in the spinal cord of motor neurone pools innervating muscles in the regenerated axolotl hindlimb. this method allows a detailed analysis of the accuracy of reformation of neuromuscular connections. the results show that regenerated distal limb muscles are reinnervated by motor neurones in the same region of the cord as those that innervate normal control distal limb muscles but that proximal muscles ... | 1987 | 3652997 |
| neuronal structure and synaptic distribution of a uropod closer motor neuron in the crayfish terminal ganglion. | one of the uropod closer muscles in the crayfish, the adductor exopodite, is innervated by two large identified motor neurons. they were injected intracellularly with horseradish peroxidase or nickel chloride to reveal the structure and distribution of the input and output synapses using electron microscopy. the development of nickel with rubeanic acid greatly improved the tissue preservation at the ultrastructural level compared with ammonium sulphide. cell bodies of the motor neurons lying in ... | 1987 | 3585417 |
| structure and localization of synaptic complexes in the cardiac ganglion of a portunid crab. | the cardiac ganglion of portunus sanguinolentus exhibits spontaneous rhythmic activity when isolated. the ganglion contains five large and four small intrinsic neurons and is innervated by three pairs of fibres originating in the thoracic ganglia. we have identified the processes of the large neurons in electron micrographs by injecting these cells with two electron-dense markers, horseradish peroxidase (hrp) and procion rubine (pr). in addition we have studied the processes of the four smaller ... | 1987 | 3585415 |
| detection and identification of plant viruses by elisa using crude sap extracts and unfractionated antisera. | a simple and rapid procedure of enzyme immunoassay (pta-elisa) was used to detect and identify viruses in individual plants. virus antigen in crude leaf extracts was adsorbed directly to a solid-phase support, allowed to react with unfractionated antiserum and the antigen-antibody complex detected with a general purpose conjugate of protein a and enzyme. viral antigens were trapped most effectively by high bonding polystyrene microtitre plates loaded with leaf extracts prepared in carbonate buff ... | 1987 | 3558704 |
| selective retrograde labeling of lateral olivocochlear neurons in the brainstem based on preferential uptake of 3h-d-aspartic acid in the cochlea. | we have previously shown that perfusion of the gerbil cochlea with probe concentrations of 3h-d-aspartic acid (d-asp) results in immediate, selective labeling of 50-60% of the efferent terminals under the inner hair cells, presumably by high-affinity uptake. the present study was undertaken to determine the origin of these endings. twenty-four hours after cochlear perfusion with d-asp, labeled neurons were observed in the ipsilateral, and to a much lesser extent in the contralateral, lateral sup ... | 1987 | 3819032 |
| localization of optic tectal input to the ventral dendrite of the goldfish mauthner cell. | although visually evoked mauthner cell (m-cell) startle responses occur in the goldfish, the afferent projections underlying these reactions have not been previously studied. we have recorded from the m-cell while stimulating the left optic nerve and/or right optic tectum and have traced projections of the optic nerve and restricted areas of the optic tectum using hrp histochemistry and autoradiography. tectal stimulation elicits similar postsynaptic potentials (psps) in both m-cells. the respon ... | 1987 | 3815088 |
| a study of the centrifugal projections to the pigeon retina using two fluorescent markers. | the centrifugal projection to the retina of the adult pigeon was studied by the retrograde transport of diamidino yellow and fast blue. on the contralateral side in the nucleus isthmo-opticus 8400-10,900 cells were stained while in the surrounding area 1500-2800 marked ectopic cells were counted. up to 94 neurones, which are nearly all ectopic, project to the ipsilateral eye. using double-labelling we concluded that there are no cells projecting to both eyes. this study shows that in the adult p ... | 1987 | 3561854 |
| assay of enzyme effectors. | the effects of various classes of organic compounds and of metal ions on the catalytic activity of horseradish peroxidase in hydrogen peroxide-catalysed o-dianisidine oxidation and, on the activity of alkaline phosphatase in p-nitrophenyl phosphate hydrolysis have been studied. enzymic methods have been developed for determination of sulphur compounds at 10(-5)-10(-4)m, nitrogen compounds at 2 x 10(-7)-3 x 10(-5)m mercury at 3 x 10(-7) mu/ml and lead at 6 x 10(-4) mu/ml concentration. | 1987 | 18964280 |
| retrograde hrp demonstration of afferent projections to the midbrain and nest calls in the ring dove. | midbrain control of vocalization was evaluated in the ring dove by determining the major afferent inputs with retrograde tract tracing technique. horseradish peroxidase was infused into various portions of the nucleus intercollicularis, an estrogen concentrating area, which disrupts nest calls when lesioned and induces the vocalization when stimulated by estrogen. most labelled cell bodies were found in the archistriatum, including a region homologous to the mammalian amygdala. | 1987 | 3828841 |
| hrp-catalyzed bioactivation of carcinogenic hydroxamic acids. the greater reactivity of glycolyl- versus acetyl-derived hydroxamic acids. | an analysis of the hydroxamic acid oxidation reaction by h2o2 and horseradish peroxidase (hrp) was made with three pairs of hydroxamic acids. each pair consisted of the aceto- and glycolhydroxamic acid derivatives from one of three different arylhydroxylamines. the parent arylhydroxylamines were the known carcinogens, n-hydroxy-2-aminofluorene and n-hydroxy-4-aminobiphenyl and the noncarcinogen 4-chlorophenyl-hydroxylamine. all the hydroxamic acids appeared to be converted to products that were ... | 1987 | 3677221 |
| generation of excited species catalyzed by horseradish peroxidase or hemin in the presence of reduced glutathione and h2o2. | horseradish peroxidase (hrp) (ec 1.11.1.7) catalyzes the oxidation of reduced glutathione. this reaction is accompanied by light emission, which is attributed to the generation of singlet oxygen. the chemiluminescence is directly related to thiyl radical formation, as deduced from the correlation between the time course of hrp-compound ii formation and light emission in the presence of different amounts of h2o2. superoxide dismutase has an inhibitory effect on the chemiluminescence without affec ... | 1987 | 3666514 |
| motor innervation of the bursalis muscle (nictitating membrane) in the lizard callopistes maculatus. | the motor neurones which innervate the bursalis muscle of the lizard callopistes maculatus were identified by means of intra-axonal retrograde transport of horseradish peroxidase. these neurones were distributed in the oculomotor abducens and accessory abducens nuclei. in the oculomotor nucleus one group of neurones was located in the ventral subnucleus of the contralateral side while the other group was found in the dorsolateral subnucleus of the ipsilateral side. in the abducens and accessory ... | 1987 | 3631531 |
| primary projections of the lateral line nerves in adult lampreys. | primary lateralis projections in silver lampreys, ichthyomyzon unicuspis, and young adult sea lampreys, petromyzon marinus, were examined utilizing silver impregnation of degenerating fibers and transganglionic transport of horseradish peroxidase. anterior lateral line nerve afferents terminate throughout the neuropil of the electroreceptive dorsal nucleus and in the lateral neuropil of the mechanoreceptive medial nucleus. the superficial ophthalmic, buccal and recurrent rami of the anterior lat ... | 1987 | 3620897 |
| isolation and characterization of specialized regions of toad urinary bladder apical plasma membrane involved in the water permeability response to antidiuretic hormone. | antidiuretic hormone (adh) increases the apical (external facing) membrane water permeability of granular cells that line the toad urinary bladder. in response to adh, cytoplasmic vesicles called aggrephores fuse with the apical plasma membrane and insert particle aggregates which are visualized by freeze-fracture electron microscopy. aggrephores contain particle aggregates within their limiting membranes. it is generally accepted that particle aggregates are or are related to water channels. hi ... | 1987 | 3599066 |
| thermodynamics of the two step formation of horseradish peroxidase compound i. | the effects of temperature (20 to -38 degrees c), pressure (normal pressures to 1.2 kbar) and solvent (water, 60% dmso and 50% methanol) on the reaction of hydrogen peroxide or ethyl peroxide with horseradish peroxidase were studied. the formation of compound i was followed at 403 nm in a stopped flow apparatus adapted for high pressure and low temperature work. as with the alkaline form (job and dunford 1978), the neutral form of the peroxidase binds peroxide substrates in two steps. it was the ... | 1987 | 3595544 |
| retinal neurones of cyprinid fish: intracellular marking with horseradish peroxidase and correlative morphological analysis. | an isolated retinal preparation from the cyprinid fish, roach, is described, in which neurones have been labelled ionophoretically with horseradish peroxidase (hrp), and then processed in a variety of steps to provide anatomical information concerning their form, structural relationships and synaptic contacts. this includes the use of computer graphics for 3d visualisation of fine structural features and generation of views from various angles. the practical procedures that have been devised sho ... | 1987 | 3582591 |
| [study of the connections of supposed diencephalic connections of the limbic system of the lizard using the technic of axonal transport of horseradish peroxidase]. | connections of the anterior thalamic (n. dorsolateralis anterior, n. dorsomedialis) and habenular nuclei in lizards ophisaurus apodus were studied by means of hrp administration into these nuclei. it was shown that all nuclei have overlapping locations of afferent sources (basotelencephalic structures, nuclei of anterior and hippocampal commissures, lateral area of the hypothalamus, superior raphe nucleus) and overlapping projectional zones (mammillary complex, ventral tegmental area). besides c ... | 1987 | 3574549 |
| alteration of blood-brain-csf barrier in experimental meningeal carcinomatosis. a morphologic and adriamycin-penetration study. | an experimental model of meningeal carcinomatosis has been produced by subarachnoid inoculation of b16 melanoma cells into c57bl mice. injection of 10(3) viable cells was sufficient to cause 100% tumor incidence and death within a median survival time of 17 days. the tumor infiltrated diffusely the meninges of the brain and spinal cord and filled the ventricular system. electron microscopic study of the leptomeningeal tumor revealed newly formed microvessels with fenestrated endothelium. the int ... | 1987 | 3559664 |
| the use of 3,3',5,5'-tetramethylbenzidine as a peroxidase substrate in microplate enzyme-linked immunosorbent assay. | the assay conditions for the use of 3,3',5,5' tetramethylbenzidine (tmb) in microplate enzyme-linked immunosorbent assay are described. tmb is a safe (non-mutagenic) chromogen that is more sensitive than opd as a substrate for horseradish peroxidase. we describe the optimum storage and assay conditions for this chromogen. | 1987 | 3558827 |
| chemical synapses, particle arrays, pseudo-gap junctions and gap junctions of neurons and glia in the buccal ganglion of helisoma. | the nervous system of the snail, helisoma trivolvis, has been utilized for a wide range of studies of neuronal plasticity; however, the ultrastructural features of this tissue were previously unknown. the present study examined the nature of synaptic interactions of neurons and glia and considered several plasma membrane specializations of these cells. the symmetrical pair of buccal ganglia consisted of a ring of unipolar neurons surrounding a central neuropil. the neurons were separated by two ... | 1987 | 3455559 |
| enzymatic activation of the carcinogens 2-phenylethylhydrazine and 1,2-dimethylhydrazine to carbon-centered radicals. | spin-trapping experiments demonstrate that oxidation of 1,2-dimethylhydrazine and 2-phenylethylhydrazine generates a comparable yield of carbon-centered radicals when catalyzed by horseradish peroxidase - h2o2. using oxyhemoglobin as the catalyst, 2-phenylethylhydrazine oxidation generates ten times more carbon-centered radicals than 1,2-dimethylhydrazine oxidation. this result is in agreement with oxygen consumption studies from which the apparent km values of 8.0 mm and 72 mm were calculated f ... | 1987 | 3455269 |
| unusual distribution of ganglion cells in the retina of the three-toed sloth (bradypus variegatus). | 1. the distribution and size of retinal ganglion cells labelled with horseradish peroxidase (hrp) were studied in flat-mounted retinas of three-toed sloths. 2. massive injections of hrp solution were made throughout the thalamus and midbrain in anesthetized sloths in order to retrogradely label the retinal ganglion cell population. twenty to thirty h later the eyes were excised and the retinas flat-mounted and reacted with phenylenediamine-hcl and h2o2 to label ganglion cells, thus distinguishin ... | 1987 | 3455252 |
| the distinction by light and electron microscopy of two types of cone containing colorless oil droplets in the retina of the turtle. | in turtle retina the spectral types of cones can be distinguished by the characteristic colors of their oil droplets. blue cones until recently were thought to be identified by their colorless oil droplets. now it has been shown that the cones containing colorless oil droplets can be subclassified into two types: one type in which the oil droplet fluoresces under ultraviolet stimulation and the other type in which it does not. we have attempted to recognize these two subclasses of cones with col ... | 1987 | 3445479 |
| optic inputs to identified neck-muscle motoneurons of salamandra salamandra (l.): an intracellular recording study. | by means of retrograde horseradish peroxidase (hrp) transport, motoneurons innervating the musculus intertransversarius capitis superior (m.i.c.s.) of salamandra salamandra (l.) were labeled from the level of the vagus nerve to the fourth spinal nerve. the bulk of the neurons was situated between the first and the third spinal nerve. the hrp-labelings revealed smaller cells with one apical dendrite and large cells with two main dendrites originating from opposite poles of the soma. with intracel ... | 1987 | 3440828 |
| the organization of afferent input to the caudal lobe of the cerebellum of the gymnotid fish apteronotus leptorhynchus. | the caudal lobe of the cerebellum of the high frequency gymnotid fish apteronotus leptorhynchus is that region of the cerebellum lying lateral to the posterolateral sulcus. it consists of three granular masses--the eminentia granularis posterior pars lateralis, a transitional zone t, and the eminentia granularis posterior pars medialis--with their associated molecular layers. we have used the retrograde transport of wheat germ agglutinin conjugated horseradish peroxidase to study the afferent in ... | 1987 | 3439638 |
| callosal and ipsilateral cortical connections of the body surface representations in si and sii of tree shrews. | injections of horseradish peroxidase (hrp) were used to study the connections of the first and second somatosensory areas (si and sii) in tree shrews. the locations of callosally projecting neurons in si were determined by placing large injections of hrp in the si region of one cerebral hemisphere and determining the organization of si of the other cerebral hemisphere with microelectrode mapping. many callosally projecting neurons were revealed in lateral si representing the face, especially the ... | 1987 | 3423531 |
| the glial blood-brain barrier of crustacea and cephalopods: a review. | 1. the glial blood-brain barrier of invertebrates is an accessible, polarised glial layer that permits study of glial cells in their normal relations with neurons. crayfish 2. the glial "perineurium" forms the blood-brain interface in crayfish, and acts as a barrier to horseradish peroxidase (hrp) and ionic lanthanum. by contrast, the perineurium of the peripheral nervous system is relatively permeable. 3. the ionic permeability of the blood-brain interface can be studied in a sucrose gap chambe ... | 1987 | 3332691 |
| a new method of lectin histochemistry for the study of brain angiogenesis. lectin angiography. | in an attempt to analyse the kinetics of angiogenesis in the brain, we developed a new lectin-histochemical staining technique for identifying the vasculature. three horseradish-peroxidase-conjugated lectins, i.e., griffonia simplicifolia agglutinin 1 (gs1), ricinus communis agglutinin 1 (rca1) and soybean agglutinin (sba), selectively stained vascular walls in brain-tissue sections. when these lectins were injected into the circulation of ether-anesthetized animals via the pulsating left ventri ... | 1987 | 3319974 |
| peroxidase-labelled monoclonal antibodies for use in enzyme immunoassay. | desirable characteristics of enzyme-antibody conjugates for use in enzyme immunoassay are labelling uniformity, permanent availability and stability. the use of monoclonal antibodies (mcabs) for preparation of enzyme conjugates, in place of polyclonal antibodies, ensures labelling uniformity and permanent availability. the problem of stability still exists. monoclonal antibody-horseradish peroxidase (mcab-hrpo) conjugates produced in our laboratory showed variable stability. after extensive test ... | 1987 | 3316282 |
| desferrioxamine as an electron donor. inhibition of membranal lipid peroxidation initiated by h2o2-activated metmyoglobin and other peroxidizing systems. | desferrioxamine (dfo) involvement in several peroxidative systems was studied. these systems included: a) membranal lipid peroxidation initiated by h2o2-activated metmyoglobin (or methemoglobin); b) phenol-red oxidation by activated metmyoglobin or horseradish peroxidase (hrp): c) beta-carotene-linoleate couple oxidation stimulated by lipoxygenase or hemin. desferrioxamine was found to inhibit all these systems but not ferrioxamine (fo). phenol-red oxidation by h2o2-horseradish peroxidase was in ... | 1987 | 3149948 |
| distribution of motoneurones innervating extraocular muscles in the brain of the marmoset (callithrix jacchus). | extraocular muscle motoneurones were localised in the oculomotor nucleus (on), trochlear nucleus (tn) and abducens nucleus (an) in the marmoset brain using the horseradish peroxidase (hrp) retrograde labelling technique. hrp pellets injected into individual extraocular muscles revealed one or more groups of labelled neurones occupying discrete loci within these nuclei. relatively little overlap of motoneurone pools was observed, except in the case of the inferior oblique and superior rectus musc ... | 1987 | 3142199 |
| lectin binding patterns in amphibian epidermis. | a battery of 6 different horseradish peroxidase conjugated lectins has been employed for structural localization of glycoconjugates in amphibian epidermis. lens culinaris (lca) lectin stained the basal membrane and gave no significant reaction on the epidermal layers. canavlia ensiformis (con a) and griffonia simplicifolia ii (gs ii) lectins bound the keratinocyte cytoplasm and the basal membrane as well. ulex europaeus i (uea i) lectin had only reactivity with flask cells. griffonia simplifolia ... | 1987 | 3105215 |
| the relationship between ionization potential and prostaglandin h synthase-catalyzed binding of aromatic hydrocarbons to dna. | a series of polycyclic aromatic hydrocarbons (pah) with ionization potentials (ip) ranging from 7.7 to 6.96 ev were examined for their ability to bind to dna in vitro via the prostaglandin h synthase (phs)-catalyzed co-oxidation of pah. a relatively low ip was required for significant binding of pah to dna to occur. pah with ip below approximately 7.35 ev were active in the binding reaction, while those with ip at or above 7.35 ev exhibited much lower levels of binding. among the pah which bound ... | 1987 | 3102084 |
| horseradish peroxidase: a useful tool for modeling the extra-hepatic biooxidation of exogens. | | 1987 | 3101748 |
| glutathione conjugate formation without n-demethylation during the peroxidase catalysed n-oxidation of n,n',n,n'-tetramethylbenzidine. | the mechanism of peroxidative n-dealkylation of alkylamines proceeds via one-electron oxidation to the iminium cation which reacts with water to give the n-hydroxymethyl derivative which decomposes to formaldehyde and the n-demethylated product. this reaction is normally inhibited by glutathione by reduction of the cation radical with subsequent formation of oxidized glutathione (gssg) with oxygen uptake. it was found that the horseradish peroxidase catalyzed n-demthylation of n,n,n',n'-tetramet ... | 1987 | 3028652 |
| lectin histochemistry of plaques and tangles in alzheimer's disease. | biotinyl derivatives of several lectins and avidin-horseradish peroxidase were used to study the localization of glycoconjugates in amyloid plaques and in neuritic tangles in brains of patients with alzheimer's disease (ad), downs syndrome (ds) and gerstmann-sträussler syndrome (gss). the lectins tested recognize the following residues: beta-d-galactosyl [ricinus communis agglutinin 120, (rca-1) and peanut agglutinin, (pna)]; alpha-d-galactosyl [griffonia simplicifolia agglutinin (gsa)]; alpha-d ... | 1987 | 2955631 |
| the time-course of appearance and net accumulation of horseradish peroxidase (hrp) presented orally to rainbow trout salmo gairdneri (richardson). | 1. a sensitive enzyme-linked immunosorbent assay (elisa) technique was used in order to determine horseradish peroxidase (hrp) uptake from the rainbow trout gut. 2. hrp was detected in blood plasma and various tissues within 15 min of oral intubation. 3. the time-course of net accumulation (uptake-degradation) over a 75 min period was recorded. 4. the presence of hrp reached a maximum in the body tissues approximately 45 min after intubation and on a ng/g weight basis the order of accumulation w ... | 1987 | 2892625 |
| characterization of endocytic compartments using the horseradish peroxidase-diaminobenzidine density shift technique. | we have employed a modification of the horseradish peroxidase (hrp)-diaminobenzidine density shift technique of courtoy et al. (j. cell biol., 1984, 98:870-876) to examine the biochemical properties of the endosome. this organelle is involved in receptor recycling and the sorting of internalized receptor ligand complexes. transferrin covalently bound to hrp was used to place peroxidase activity specifically within the endosome. the peroxidase-catalyzed polymerization of diaminobenzidine within t ... | 1987 | 2878931 |
| a comparison between the connections of the amygdala and hippocampus with the basal forebrain in the macaque. | autoradiographic experiments indicated that the amygdala projects to division ch3 and ch4 of the basal forebrain (nomenclature from mesulam et al. 1983) and the olfactory tubercle. the heaviest of these amygdaloid outputs arose from the lateral basal, accessory basal, central, and medial amygdaloid nuclei, each with a slightly different pattern of distribution from that of the other. injections of the retrograde tracer horseradish peroxidase (hrp) into the amygdala revealed dense reciprocal proj ... | 1987 | 2820778 |
| coupling of different isoenzymes of horseradish peroxidase influences the sensitivity of enzyme immunoassay. | labelling of igg with various hrp isoenzymes purified by preparative isoelectric focussing influences the yield and the specific activity of the conjugates. alkaline isoenzymes were preferably coupled by glutaraldehyde whereas application of the periodate method additionally formed relatively large quantities of conjugates with acidic isoenzymes of a high purity number, but a low specific activity. in an enzyme immunoassay for alpha fetoprotein the detection limit can be varied by a factor of 6 ... | 1987 | 2451517 |
| development of the basilar pons in the north american opossum: dendrogenesis and maturation of afferent and efferent connections. | the present study provides data on temporal factors that may play a role in the development of precerebellar-cerebellar circuits in the north american opossum. in this study the basilar pons and cerebellum are analyzed from birth, 12-13 days after conception, to approximately postnatal day (pd) 80 at which time the brainstem and cerebellum have a mature histological appearance. in nissl preparations, the basilar pons was first seen at pd 7 as a small cluster of tightly packed cells. analysis of ... | 1987 | 2441628 |
| pathway formation and the terminal distribution pattern of the spinocerebellar projection in the chick embryo. | pathway formation and the terminal distribution pattern of spinocerebellar fibers in the chick embryo were examined by means of an anterograde labelling technique with wheat germ agglutinin conjugated horseradish peroxidase (wga-hrp). spinocerebellar fibers, which originate in the lumbar spinal cord and are located in the marginal layer of the spinal cord, reach the dorsal part of the cerebellar plate on embryonic day (e)8. on the way to the cerebellum the fibers form one distinct bundle, that s ... | 1987 | 2441626 |
| circumferential cells of the developing rana catesbeiana lumbar spinal cord. | neural elements in the lumbar enlargement of the developing rana catesbeiana spinal cord were labelled by placing chips of dessicated horseradish peroxidase (hrp) into various lesions of the spinal cord. of the elements labelled in the lumbar enlargement, a population of cells circumjacent to the gray matter was seen to be distinct from all others on the basis of their morphology, position and their putative embryonic origin. one cell type not previously described was a large circumferential cel ... | 1987 | 2441625 |
| [detection of iga defects in blood donors using an enzyme immunoassay]. | an enzyme immunoassay is presented for screening blood donors for iga-defects using horseradish peroxidase conjugated iga. fife of about 4,700 screened donors was found to be iga-deficient. with another eia using horseradish peroxidase conjugated monoclonal anti-iga and iga coated microplates the iga-concentration in serum of these selected donors was determined. | 1987 | 2440782 |
| morphology of geniculocortical axons in turtles of the genera pseudemys and chrysemys. | an analysis has been made of the morphology of axons in the geniculocortical pathway of turtles using the anterograde transport of horseradish peroxidase in both in vivo and in vitro preparations. following injections of hrp into the dorsolateral thalamus, labeled axons could be traced from the dorsal lateral geniculate complex to the telencephalon. they are unbranched and free of varicosities within the diencephalon. they travel in the dorsal peduncle of the lateral forebrain bundle, through th ... | 1987 | 2437831 |
| an electron spin resonance study of free radical intermediates in the oxidation of indole acetic acid by horseradish peroxidase. | the oxidation of indole-3-acetic acid by horseradish peroxidase was studied using the spin traps t-nitrosobutane and 5,5-dimethyl-1-pyrroline n-oxide to trap free radical intermediates. the major free radical metabolite of indole acetic acid was unambiguously determined by the use of indole-3-[2,2-2h2]acetic acid to be the skatole carbon-centered free radical. in the presence of oxygen, superoxide was also trapped. | 1986 | 3023369 |
| organization of corticogeniculate projections in the turtle, pseudemys scripta. | the efferent pathways from the visual cortex to the dorsal lateral geniculate complex of turtles have been studied by using the orthograde and retrograde transport of horseradish peroxidase (hrp). injections of hrp in the lateral thalamus retrogradely label neurons throughout the visual cortex. the majority of labeled neurons have somata in layer 2 of the lateral part of dorsal cortex (d2); a minority have somata in layer 3. labeled neurons in layer 2 tend to have vertically oriented, fusiform s ... | 1986 | 3805361 |
| map of retinal position onto the cross section of the optic pathway of goldfish. | the position of a retinal cell is defined by the two polar coordinates: r, the distance from the optic disc, and theta, the angular (or clock-face) position. axons of similar theta value were labeled by the punctate application of horseradish peroxidase (hrp) to optic axons in the retina, and axons of similar r-value were labeled by the application of this same marker to a tectal fascicle. labeled axons were traced in serial transverse sections of the optic pathway from the retina to the tectum ... | 1986 | 3805359 |
| distribution of vestibular afferents that innervate the sacculus and posterior canal in the gerbil. | the central distribution of afferents that innervate the macula of the saccule and the crista of the posterior canal was assessed in the gerbil following the direct injection of horseradish peroxidase (hrp) separately into the sensory neuroepithelia of each peripheral receptor organ. ganglion cells innervating the posterior canal were located in the caudal part of the inferior ganglion, while those cells innervating the saccule were located in the rostral part of the inferior ganglion, scattered ... | 1986 | 3491843 |
| proton and nitrogen-15 nmr spectroscopic studies of hydrogen ion-dependent pseudo-halide ion binding to chloroperoxidase. | the proton nuclear magnetic resonance spectra of several chloroperoxidase-inhibitor complexes have been investigated. titrations of chloroperoxidase with azide, thiocyanate, cyanate, or nitrite ions indicate that only the chloroperoxidase-thiocyanate complex exhibits slow ligand exchange on the 360-mhz nmr time scale. the temperature dependence of the proton nmr spectra of the complexes suggests that, although the complexes are predominantly low-spin ferric heme iron, a spin equilibrium is prese ... | 1986 | 3023353 |
| immunocytochemical localization of serotonin in intracellularly analyzed and dye-injected ganglion cells of the turtle retina. | combining intracellular recording techniques with immunocytochemistry revealed the existence of serotonin within a few ganglion cells of the turtle retina. lucifer yellow was intracellularly injected into physiologically classified ganglion cells. sections containing labeled somata were subsequently incubated with an antibody directed against serotonin and immunoreactivity was revealed using horseradish peroxidase as a marker. in 3 ganglion cells the two markers were co-localized. these cells ra ... | 1986 | 3543751 |
| distribution and ultrastructure of synapses on a premotor local nonspiking interneuron of the crayfish. | many premotor local nonspiking interneurons are involved in the control of the uropod movements of a crayfish. one of these interneurons was impaled intracellularly, characterized physiologically, and then labeled by intracellular horseradish peroxidase (hrp) injection to examine the distribution and ultrastructure of synapses. depolarization of this interneuron by a current injection excited the closer motoneurons of the uropod and inhibited its opener motoneurons, but hyperpolarization had no ... | 1986 | 3794006 |
| 18o studies of the peroxidase-catalyzed oxidation of n-methylcarbazole. mechanisms of carbinolamine and carboxaldehyde formation. | chloroperoxidase, horseradish peroxidase, hemoglobin, myoglobin, lactoperoxidase, and microperoxidase catalyzed the ethyl hydroperoxide-dependent oxidation of n-methylcarbazole to n-(hydroxymethyl)carbazole and n-formylcarbazole as major products. mass spectral analysis of the n-(hydroxymethyl)carbazole formed during the peroxidase-catalyzed n-demethylation of n-methylcarbazole in 18o-enriched medium indicated partial incorporation (7.5-25.9%) of solvent water oxygen into the carbinolamine inter ... | 1986 | 3782097 |
| a tetrazolium method for staining peroxidase labels in blotting assays. | a sensitive staining method of horseradish peroxidase-labeled immunoglobulins on nitrocellulose membrane was established by employing a reaction chain leading to formazan formation with phenol as a substrate of peroxidase and nadh as a hydrogen donor to reduce nitro blue tetrazolium. higher concentrations of nadh relative to phenol were necessary to increase the intensity of staining and to ensure a wide dose-response range of color production with respect to the applied enzyme activities. by an ... | 1986 | 2431066 |
| retinal topography in the optic tract of adult goldfish. | the present study was aimed at determining how transformations in fiber order establish a retinal topography in the optic tract of adult carassius auratus. horseradish peroxidase was applied to the optic nerve or retina, and the pathways of labeled axons originating from retinal annuli or wedges were analysed in reconstructed serial-sections and wholeamounts of the optic pathway. the age-related fiber order of the optic tract involves a rotation of the optic pathway that begins near the chiasm, ... | 1986 | 3822125 |
| localization of motoneurons innervating the extraocular muscles in salamandra salamandra l. (amphibia, urodela). | the central innervation patterns of the extraocular muscles were investigated in the european fire salamander salamandra salamandra l. by means of the horseradish peroxidase method. the ipsilateral portion of the nucleus nervi oculomotorii, which is located in the rostral ventral tegmentum mesencephali, supplies the musculi recti inferior and medialis and the musculus obliquus inferior without a clear somatotopic representation of the motoneurons. the musculus rectus superior is innervated mainl ... | 1986 | 3805351 |
| hammett rho sigma correlation for reactions of horseradish peroxidase compound ii with phenols. | the rates of reduction of horseradish peroxidase compound ii by p-methoxyphenol (4-hydroxyanisole) have been studied from ph 6.0 to 10.5. the kinetics are influenced by an acid group of pka 8.7 on compound ii. the acidic form of compound ii is reactive; the basic form is not. only the electrically neutral, unionized form of p-methoxyphenol is reactive. fifteen different phenols were reacted with compound ii at either ph 7.6 or ph 7.0 (three of them at both ph's). rate constants varied from zero ... | 1986 | 3800384 |
| cooxidation of styrene by horseradish peroxidase and glutathione. | styrene is oxidized to styrene oxide and benzaldehyde in the presence of glutathione, horseradish peroxidase, and hydrogen peroxide. styrene oxide is not formed if any one of these reaction components is omitted or if oxygen is exluded from the reaction. the oxygen atom in the styrene oxide derives from molecular oxygen rather than from hydrogen peroxide. oxidation of trans[1-3h]styrene yields the epoxide in which the deuterium stereochemistry is completely scrambled. the results indicate that t ... | 1986 | 3785143 |