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[aspergilli and chronic lung diseases].in this study, mycological examinations of morning sputum of patients with chronic bronchitis and bronchial asthma has been performed. specimens were also examined from patients with lobar pneumonia as a control. a fumigatus and a. niger has been isolated from patients chronic bronchitis in frequency of 4.5% and 0.8% respectively. these 2 fungi has also been isolated from patients with bronchial asthma in frequency of 24.5% (a. fumigatus) and 2.4% (a. niger). the frequency of isolation of a. fum ...19826755197
[experimental finding with a new type of fungicide]. 19826755244
enzymic formation of di-d-fructose 1,2'; 2,1' dianhydride from inulobiose by aspergillus fumigatus.the enzyme of aspergillus fumigatus which produces di-d-fructose 1,2'; 2,1' dianhydride (difructose anhydride i) (tanaka et al., 1979) was investigated for its mode of action. fractionation and purification of the enzyme showed that it produced difructose anhydride i not from inulin but from inulobiose by the reverse reaction of the partial hydrolysis of difructose anhydride i.19826757245
laser microbeam studies on fungal tissues. 19826757676
cerebral mass in a leukemic patient. 19826759454
a comparison of surface and cytoplasmic antigens of aspergillus fumigatus in an enzyme-linked immunosorbent assay (elisa). 19826759936
studies on air-borne fungi at qena. iii. thermophilic fungi. 19826759940
[marfan's syndrome complicated by fibrous-cavernous pulmonary tuberculosis and aspergillosis]. 19826760187
saprophytic invasion of infarcted pulmonary tissue by aspergillus species.saprophytic invasion of infarcted pulmonary tissue has received only passing reference in published reports. five patients with colonisation of pulmonary infarcts by aspergillus are described, with discussion of the clinical and histopathological features. the characteristic features were widespread invasion of dead tissue by fungal hyphae, in three cases with liquefaction resulting in the production of a cavitated lesion. there was no evidence that the vascular thrombosis and the consequent inf ...19826760449
antitumor effect of heat-killed aspergillus fumigatus mycelium in a mouse model.a suspension of heat-killed aspergillus fumigatus mycelium inhibited the growth of a chemically-induced mouse bladder tumor (mbt). tumor growth was inhibited when the mycelium was injected into mice in a mixture with the tumor cells, when injected into growing tumors, and when introduced ip at the time tumor cells were injected into the hind leg muscle. in the concentrations that affected tumor growth no toxicity of the fungus preparation was observed. the fungal suspension was more effective ag ...19826760960
studies on the composition and antibacterial activity of uterine fluid from mares.forty uterine fluid samples were obtained during oestrus and dioestrus of successive cycles from 4 mares classified as resistant to bacterial infection of the uterus. the flushings were assayed for peroxidase activity and ability to influence phagocytosis by polymorphonuclear neutrophils. uterine fluid volumes and protein concentrations and serum oestrogen values were similar during oestrus and dioestrus. serum progesterone concentrations were depressed during oestrus. equine neutrophils adheren ...19826762436
[precipitins in sarcoidosis]. 19826763093
[solid-phase radioimmunoassay for measurement of igg, iga and igm antibodies against aspergillus fumigatus]. 19826763647
[effect of filtrates from the aspergillus fumigatus cultures on the development of experimental emphysema in rats based on the model of papain-induced emphysema]. 19826763686
isolation and ultrastructural examination of conidial wall components of coccidioides and aspergillus.the role of the propagule in the initiation of fungal disease is not fully appreciated. knowledge of the nature of early interactions between infectious propagules and host tissue during onset of respiratory mycoses is particularly scanty. this stems partly from lack of sufficient data on the structure, chemical composition and immunological activity of the microbial surface. in this study, a technique is described for isolation and subsequent ultrastructural examination of the surface wall comp ...19826764024
[mycoses caused by fungal spores in indoor air].fungal spores in indoor air may cause mycoses and allergies in man. the following different types of exposure are interesting from the epidemiological view-point: (a) the occupational exposure (work done with fungi-contaminated material), (b) the accidental exposure (formation and growing of fungi by constructive defects), and (c) the inconspicuous constant exposure (as part of lifestyle, e.g. by plants or small pets). it is stressed that the only way to characterize the indoor air quality with ...19826764028
immune complexes and humoral response to pseudomonas aeruginosa in cystic fibrosis.we studied the humoral immune status of 51 patients with cystic fibrosis (cf) as compared to 25 patients with other respiratory diseases (rd). cf patients had higher serum concentrations of igg and iga (p less than 0.001), c5, and ch50 (p less than 0.05), than did rd patients. twenty-three % of cf patients had increased ige concentrations. of 32 cf and 1 rd patients colonized with mucoid pseudomonas aeruginosa (pa), 91% had serum precipitins to pa, whereas no precipients were found in patients n ...19806766286
air-borne fungi in the air of barcelona (spain). iii. the genus aspergillus link.during a survey on the presence of species of the genus aspergillus in the air of the city of barcelona (spain), the following species were identified: aspergillus flavus link, a. niger van tieghem, a. fumigatus fresenius, a. clavatus desmazières, a. terreus thom, a. chevalieri (mang.) thom et church, a. niveus bloch, emend. thom et church, a. ochraceus wilhelm, a. versicolor (vuillemin) tiraboschi, and a. amstelodami (mang.) church et thom.19806770271
[about 35 cases of pleural aspergillosis (author's transl)].considering 36 observations, the authors show the relative infrequency of pleural aspergillosis localizations by comparison with pulmonary aspergillomas. twenty four pleural aspergilloses developed on post-operative residual cavities, particularly after pulmonary exeresis and not only for aspergilloma; 11 forms were apparently primitive after therapeutical pneumothorax; 3 anatomo-clinical forms were found, aspergillomas and pyothoraxes were the most frequent. pleural involvement can be associate ...19806770358
cellulase induction in aspergillus fumigatus m 216. 19806771206
diagnosis of invasive aspergillosis by passive hemagglutination assay of antibody.sheep red cells treated with concanavalin a and sensitized with a partially purified aspergillus antigen were used to detect antibody to aspergillus by passive hemagglutination (pha). sera from eight patients with aspergillomas or allergic aspergillosis had pha titers of greater than or equal to 1:800 and antibody detectable by immunodiffusion (id). of 122 hospitalized cancer patients without invasive aspergillosis, 118 had titers of less than or equal to 1:80, 86 of < 1:10, two of 1:160, and tw ...19806772712
preparation of aspergillus fumigatus antigens and their analysis by two-dimensional immunoelectrophoresis.the water-soluble components of aspergillus fumigatus mycelium were partially separated by fractional precipitation with ammonium sulphate. the total protein and neutral-sugar content were determined for each of the four fractions prepared and their immunological activity was examined by double diffusion. partial chemical characterisation of these isolates by polyacrylamide-gel electrophoresis was linked to their precipitability by allowing the separated components to diffuse from the gel into a ...19806774095
the detection of circulating antibodies against antigens from three strains of aspergillus fumigatus. 19806775222
[inactivation of viruses and bacteria in sewage sludge by gamma radiation (author's transl)].the kinetics of inactivation and the resistance to gamma radiation of microorganisms usually to be found in raw sludge were examined with five viruses, three bacteria and a fungus serving as prototypes in comparative studies. all these infectious agents could reliably be inactivated by gamma rays in raw sewage sludge but they were clearly more resistant to gamma rays compared to irradiation in a liquid suspension. the reduction of the virus content required a much higher radiation dose compared ...19806775456
characterization of antigens from aspergillus fumigatus. v. reactivity in immunodiffusion tests with serums from patients with aspergillosis caused by aspergillus flavus, a. niger, and a fumigatus.antigen-rich mycelial extract (me) and fractions of aspergillus fumigatus were compared with culture filtrates of a. fumigatus. a. flavus, and a. niger for their abilities to detect antibody in serums from patients with confirmed aspergillosis caused by these fungi. in double immunodiffusion tests, homologous antigen preparations were more effective than heterologous preparations in detecting precipitins. mycelial extract of a. fumigatus was more effective than a. fumigatus culture filtrate in ...19806776857
serodiagnosis of aspergillus fumigatus antibody in migratory ducks.migrating duck populations in wisconsin were evaluated during a one-year period for exposure to aspergillus fumigatis by two serodiagnostic methods: immunodiffusion and countercurrent immunoelectrophoresis. peak antibody reactivity to sporulating culture extracts of the fungus was detected in serum samples collected in winter. antibody levels did not differ between male and female or adult and juvenile populations.19806776945
the preparation and partial purification of fractions from mycelial fungi with antigenic activity. 19806777666
a notable aspergillus from a mortal aspergilloma of the lung. new aspects of the epidemiology, serodiagnosis and taxonomy of aspergillus fumigatus.different basic mycological observations were made in relation to a fatal case of aspergilloma of the lung in a 45-year-old male. cultural examination of the sputum and autopsy material from the lung revealed the predominant presence of a white fungus along with a scarce growth of the typical aspergillus fumigatus. the white isolate produced conidial heads almost similar to that of a. fumigatus but with apparent septation in the phialides. with the help of the immunodiffusion test and use of the ...19806779440
plasma progesterone concentration in the bovine before abortion or parturition in pregnant animals exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus.pregnant cows in the 4th and 5th month of the gestation were exposed to sarcocystis cruzi, campylobacter fetus, or aspergillus fumigatus. plasma progesterone concentrations were determined at intervals from before the cows were exposed until they had aborted or calved. the plasma progesterone concentration of the exposed pregnant cattle remained at 3.95 +/- 2.0 ng/ml until 24 to 48 hours before abortion or parturition, when it decreased to below 1.00 ng/ml. this pattern was similar for cattle ex ...19806782916
hypothyroidism with epiphysial dysgenesis. 19816784510
fungal pneumonia (part 4): invasive pulmonary aspergillosis. 19816788456
positive immediate skin tests in cystic fibrosis: a possible role for pseudomonas infection.a review was made of the medical records of 49 patients from whose sputum mucoid pseudomonas aeruginosa had been isolated over a 20-month period. this showed that 31 of 42 had positive immediate skin prick tests to common antigens. 21 had positive reactions to aspergillus fumigatus antigens and 23 had precipitins to a. fumigatus antigen. 36 patients had had frequent courses of antibiotics and airway obstruction was present in 47. these results have prompted the hypothesis that the positive skin ...19816789859
response of turkey poults to aerosolized spores of aspergillus fumigatus and aflatoxigenic and nonaflatoxigenic strains of aspergillus flavus.groups of 3-week-old turkey poults were exposed to aerosols containing spores of either aspergillus fumigatus or aflatoxigenic or nonaflatoxigenic strains of aspergillus flavus. approximately 5 x 10(5) spores of a. fumigatus/gm of lung tissue killed about 50% of the turkey poults with a. funigatus, whereas doses approximating this level with either of the a. flavus strains did not cause any mortalities for up to 8 weeks postexposure. fungi were isolated from lung tissue of 60% of the birds survi ...19816791628
serological diagnosis of pulmonary aspergillosis.methods for the preparation of antigens from clinically isolated cultures of aspergillus were standardized. sera from 25 suspected cases of pulmonary aspergillosis were tested against antigens prepared by us, from 4 strains of a. fumigatus and one strain of a. flavus, using the ouchterlony double diffusion and immunoelectrophoretic techniques. of the 25 sera, 18 reacted positively with antigens of a. fumigatus, one with a. flavus and 2 with both these species. antigens of two non-pathogenic aspe ...19816792543
summary and analysis of the mycology proficiency testing survey results of the college of american pathologists. 1976-1979.the results of the cap mycology proficiency testing survey programs for 1976 through 1979 are summarized and analyzed. there has been a slight, but definite, improvement in participant performance over this four year period and considerable improvement over the performance of 1972. the problems in analyzing participant performance are reviewed. the survey program itself is examined in the light of the stated objectives, and areas for improvement noted.19816792904
serological reactivity of duck sera to three electrophoretically characterized extracts of aspergillus. 19816793296
serodiagnostic value of culture filtrate antigens from aspergilli with septate phialides.culture filtrate antigens from 6 typical isolates of aspergillus fumigatus, 6 isolates with septate phialides, and isolates of a. fumigatus var. ellipticus, a. fishceri, a. flavus and a. niger were studied in tests with 2,100 samples of serum originating from 1,452 patients suffering from miscellaneous diseases, including 60 cases of aspergillosis, 850 samples of serum from apparently healthy blood donors and 522 sera referred to us as positive for c-reactive protein. the culture filtrate antige ...19826801788
diffusion-in-gel enzyme-linked immunosorbent assay (dig-elisa) for detection of class-specific antibodies to aspergillus fumigatus and candida albicans. 19816801921
xenon-133 evidence of bronchopleural fistula healing during treatment of mixed aspergillus and tuberculous empyema. 19826809909
detection of aspergillus antigen and anti-aspergillus precipitating antibodies by a new method: the radial immunoelectro-osmophoresis (rieop).a new method of immunoprecipitation-in-gel, the radial immunoelectro-osmophoresis (rieop) has been applied to detect precipitating anti-aspergillus fumigatus antibodies and to study the antigens present in various antigenic extracts of this fungus. the results obtained with this method have been compared with those obtained when applying the comparative double diffusion (cdd) and the immunoelectro-osmophoresis (ieop). the data presented prove that the rieop method is more sensitive than cdd and ...19826811158
comparison of aspergillus fumigatus and aspergillus umbrosus antigens in serological tests of farmer's lung.a comparison was made between aspergillus fumigatus and a. umbrosus antigens regarding their usefulness in serological tests of farmer's lung. mycelial and culture fluid antigens of both microbes were used to test sera of 30 finnish farmer's lung patients for antibodies in the elisa and in the gel precipitation (gp) test. antibodies to a. umbrosus were more common than to a. fumigatus; this was especially evident in elisa, elisa gave more positive results with all four antigens than gp. mycelial ...19826812448
rapid enzyme-linked immunosorbent assay (elisa) for aspergillus fumigatus antibodies.a rapid enzyme-linked immunosorbent assay (elisa) where component incubation periods were shortened to one hour, was compared with agar gel double diffusion (agdd) and a standard elisa procedure for detecting antibodies to aspergillus fumigatus in 28 asthmatic patients with suspected allergic aspergillosis. using two a fumigatus antigens the rapid elisa compared well with agdd and the standard elisa method. eleven sera that reacted with both antigens in agdd were all positive against antigen 1 i ...19826813358
serology of aspergillus granuloma.a total of 21 cases of granuloma caused by aspergillus species were encountered during the period 1972-79. the organs involved were nasal and paranasal sinuses, brain, orbit, subcutaneous tissue of check, lungs and endocardial valve in the decreasing order of their frequency. aspergillus flavus was the main etiological agent. immunodiffusion tests with various aspergillus species as antigen showed a positively of 17 out of 18 (94.4 per cent) cases. the role of precipitins in diagnosis and progno ...19826813743
serum antibodies to aspergillus fumigatus in patients with pulmonary aspergillosis detected by immunofluorescence.aspergillus antibodies of the igg, iga and igm class were determined by the indirect immunofluorescence (iif) technique in sera from 11 patients with proven pulmonary aspergillosis (group i), 11 patients with suspected pulmonary aspergillosis (group ii), 34 patients with chronic obstructive lung disease (group iii), 29 patients with lung infiltrates of non-fungal aetiology (group iv), and 94 blood donors (group v). the antigen was cryostate sections of formaldehyde fixed mycelium. igg antibody t ...19826814182
rapid serological diagnosis of aspergillus fumigatus infection of the frontal sinuses and nasal chambers of the dog.aspergillus fumigatus precipitins in dog sera were detected by counterimmunoelectrophoresis (cie). the procedure gave results within 90 minutes compared with 72 to 96 hours required in agar-gel double diffusion. culture-filtrate antigens from two-week-old cultures of a fumigatus and two-day-old mycelial antigens produced the strongest reactions in cie tests and the former antigen also revealed high titres in tests with serum from seven of 14 dogs with a fumigatus precipitins. serum from these 14 ...19826815743
extracellularly proteolysing aspergillus fumigatus and its interaction with specific immune serum in the serum-albumin agar. a preliminary report.serum-albumin agar with the initial ph values of 5.0 or 7.0 allows diffusion and precipitation of a. fumigatus antigens (culture filtrate) and antibodies. in the zone of extracellular proteolysis by a living culture of an atypical a. fumigatus strain, precipitin bands only against a specific immune serum were found in this medium with an initial ph of 7.0. how far under such complex substrate conditions including active extracellular proteolysis, an immune reaction can take place is open to disc ...19826819740
[the effects of various tetrahydro-1,3,5-thiadiazines as antifungal agents]. 19826820681
marijuana smoking and fungal sensitization.the possible role of marijuana (mj) in inducing sensitization to aspergillus organisms was studied in 28 mj smokers by evaluating their clinical status and immune responses to microorganisms isolated from mj. the spectrum of illnesses included one patient with systemic aspergillosis and seven patients with a history of bronchospasm after the smoking of mj. twenty-one smokers were asymptomatic. fungi were identified in 13 of 14 mj samples and included aspergillus fumigatus, a. flavus, a. niger, m ...19836833678
immediate-type reactions in patients with allergic bronchopulmonary aspergillosis.allergic features of 38 patients with allergic bronchopulmonary aspergillosis (abpa) were reviewed. these features included skin reactivity to other inhalant antigens and to molds other than aspergillus fumigatus (af) plus clinical manifestations of rhinitis, conjunctivitis, asthma, eczema, urticaria, anaphylaxis, food allergy, and drug allergy. abpa patients have a high degree of allergic reactivity in all these clinical features, in particular, clearly documented food allergy. these findings d ...19836853923
a radioimmunoassay index for allergic bronchopulmonary aspergillosis.differentiating patients with allergic bronchopulmonary aspergillosis from those with asthma who have immediate cutaneous reactivity to aspergillus fumigatus may be difficult. both groups have ige and igg antibodies against aspergillus antigen. we describe methods for establishing ige and igg antibody indices using sera from asthmatic patients as a control serum pool. the ige and igg antibody indices are usually both positive and at least one index was positive in all patients during the acute s ...19836859719
antibodies against aspergillus fumigatus in bronchial secretions from patients without aspergillosis.antibodies to aspergillus fumigatus were investigated by indirect immunofluorescence (iif) and enzyme-linked immunosorbent assay (elisa) in bronchial secretions (bs) and sera from 40 patients with neoplasia, lung infiltrates of non-fungal aetiology or chronic bronchitis. by iif, igg aspergillus antibodies were found in 23 samples of bs (58%) (titre range 10-40) and in all 40 sera (titre range 10-1280). iga aspergillus antibodies were detected in 33 samples of bs (83%) (titre range 10-160) and in ...19836880743
aspergillosis of the nose and paranasal sinuses.aspergillosis is becoming an increasingly recognized pathogen in the sinonasal tract. the courses of two patients, one seemingly healthy and one with a terminal malignancy, are reviewed. these patients illustrate the clinical course, difficulties in diagnosis, and management of patients with aspergillosis of the sinonasal tract. aspergillus is a common endogenous contaminant of the upper respiratory tract; however, bacterial sinusitis may trigger its growth and proliferation. aspergillus fumigat ...19826953293
aspergillosis mycetoma of the maxillary antrum.aspergillus is a fungus, usually found growing as one of the many moulds of fruit, foods and nuts. this paper deals with a ball of fungus growing in the maxillary antrum of an elderly female patient presenting as a radio-opaque expansile mass. in man, aspergillus fumigatus and aspergillus flavis have both been incriminated as pathogenic species in the para nasal sinuses. spores of aspergilli become mixed with dust and can enter the ear, nose and lungs. if conditions are favourable, growth will e ...19826961938
atopic allergy, serum ige and rast specific ige antibodies in patients with cystic fibrosis.the occurrence of allergic diseases is studied in a group of 56 swiss children suffering from cystic fibrosis (cf). 27% of cf children showed allergic manifestations, and 61% of cf children had positive family history for atopic diseases. the occurrence of allergies is increased in homozygotes as well as in heterozygotes for cf. total serum ige values are clearly increased in atopic cf children, but also slightly elevated in cf patients without allergic manifestations. occurrence of specific ige ...19806967864
the effect of bacterial and fungal phospholipids on immune response to sheep erythrocytes and e. coli lipopolysaccharide.the effect of phospholipids extracted from some bacteria and fungi on the response of mice to sheep erythrocytes and e. coli lipopolysaccharide has been studied. the phospholipids from staphylococcus aureus. streptococcus faecalis, bacillus subtilis and aspergillus fumigatus augmented the humoral immune response to both antigens studied. the phospholipids from asp. fumigatus, str. faecalis and candida albicans stimulated mouse b lymphocyte proliferation in vitro. on the other hand, none of the p ...19816982033
carotid-cavernous sinus thrombosis caused by aspergillus fumigatus. case report.a case is presented of aspergillus fumigatus granuloma involving the sphenoid sinus, sella turcica, cavernous sinus, and the internal carotid artery. the diagnosis was established by a transsphenoidal biopsy. the infection proved difficult to treat and finally remitted after chemotherapy with a combination of amphotericin b, rifampin (rifampicin), and flucytosine (5-fluorocytosine). the spectrum of aspergillosis of the central nervous system is reviewed, and difficulties in treating this infecti ...19806985651
cutaneous aspergillosis with fatal dissemination in a renal transplant recipient. 19806988066
chemistry of tremorogenic metabolites. i. fumitremorgin a from aspergillus fumigatus. 19806988091
an epidemic of extrinsic allergic alveolitis caused by tap water. 19806988107
prevalence of precipitins in groups at risk of developing hypersensitivity pneumonitis.a study was made of the prevalence of serum precipitins to micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus, and pigeon serum in population groups suspected to be at high risk for the development of hypersensitivity pneumonitis. pigeon breeders' sera contained precipitins mainly to pigeon serum (38%) and a. fumigatus (18%). occupants of homes in which forced air heating systems were investigated for the presence of microorganisms reacted mostly with m. faeni (13%) and a. f ...19806988108
[aspergillus fumigatus infection of the lung in cystic fibrosis (author's transl)].in an 8-year-old girl with cystic fibrosis aspergillus fumigatus infection of the lung was diagnosed. pot-plant soil as reservior of aspergillus fumigatus was shown to be the source of inhalable aspergillus fumigatus units within the clinic. cystic fibrosis mucus of the patient acted as nutritive substrate for aspergillus fumigatus. consequent exclusion of pot plants in housing and hospital rooms is considered necessary for prevention of aspergillus fumigatus infection in patients with cystic fi ...19806988190
[taxonomic and ecological and physiological characteristics of aspergillus fumigatus]. 19806988681
fungal infection of the brain: an increasing threat.a 79-year-old, diabetic man with an ear infection had a temporal lobe abscess from which aspergillus fumigatus was cultured. he died despite treatment with amphotericin and rifampin, removal of the abscesss, and left mastoidectomy. because of the ever-increasing number of elderly debilitated patients, the frequency of mild diabetes in the aged, the prevalent use of steroids, antibiotics, cytotoxics and immunosuppressives, and the ubiquity of the organism, aspergillus infections of the brain have ...19806988973
aspergillus nidulans and aspergillus fumigatus as causal agents of bovine mastitis.five cases of bovine udder aspergillosis diagnosed clinically and confirmed by repeated demonstration of fungal hyphae in koh preparations and isolation of the organism from milk sediments are described. in one case the fungus was identified as aspergillus nidulans, in the other 4 cases as a. fumigatus. the most pronounced features in altered udders were multiple abscesses with distinctive colonies of fungal hyphae in the centres, embedded in cell detritus, neutrophils and macrophages. these pur ...19806988991
the preparation of protoplasts from aspergillus fumigatus mycelium.protoplasts have been prepared from the mycelium of aspergillus funigatus, using a lytic enzyme mixture from trichoderma harzianum. a variety of experimental conditions were investigated in order to achieve optimal conditions for protoplast production. electron micrographs showed the preparations to be free of cell-wall. material obtained by this procedure can be used as a source of cytoplasmic antigens for further analysis.19806988993
[ocular mycoses as a complication of hormonal therapy]. 19806989089
serologic immunodiagnosis of of invasive aspergillosis.two sensitive methods, counterimmunoelectrophoresis (cie) and enzyme-linked immunosorbent assay (elisa), for detecting antibodies to aspergilus were used to study serial specimens from patients with histologically proven invasive aspergillosis for measurement of conversion from negative to positive immunoprecipitin reactions and changes in elisa titers during immunosuppression. sera from 12 granulocytopenic patients without invasive aspergillosis served as controls. positive cie reactions were d ...19806989934
sero-diagnosis of invasive aspergillosis: attempts to determine antigen and antibody relevance to infection.attempt was made to define antigens and antisera which might prove useful in diagnosis of invasive aspergillosis in man. a convalescent antiserum (serum from rabbits after liver infection with aspergillus fumigatus conidia) which might be more representative of immunological reaction to fungal growth in vivo, did not react in enzyme-linked immunosorbent assay with commerical antigens which are used at present in attempts to detect antibody response in systemic infections in man. however, this co ...19806990268
[pulmonary mycetomas of the aspergilloma type]. 19806990577
examination of the relationship between pathological changes, immunological response and serum protein concentrations in pregnant sheep inoculated with aspergillus fumigatus.pregnant sheep inoculated with aspergillus fumigatus conidia developed precipitating and latex agglutinating antibodies to mycelial antigens. the titres of these tended to be higher in those animals developing placental or fetal infection than in those which did not. the concentrations of total serum proteins and of albumin, alpha, beta and gamma globulins did not show any consistent changes which could be related to abortion or placental infection. lymphocyte transformation tests on whole blood ...19806991951
necrotising aspergillosis complicating fulminating viral pneumonia. 19806992331
aortotomy site infections: case presentation and review of the literature.a patient with aspergillus fumigatus infection involving an aortotomy site with multiple peripheral emboli following implantation of an aortic valve prosthesis for rheumatic heart disease is described. eleven aortotomy site infections are reviewed from the literature. eight cases were caused by fungi with aspergillus sp accounting for four cases. presenting symptoms were nondiagnostic and multiple. however, the diagnosis of aortotomy site infection should be entertained in patients who have had ...19806992578
antibodies to aspergillus fumigatus in farmers' lung patients measured by enzyme-linked immunosorbent assay (elisa).enzyme-linked immunosorbent assay (elisa) was used to measure antibodies against aspergillus fumigatus in farmers' lung patients, various other pulmonary diseases and healthy controls. both the prevalence and the mean titre of antibodies were significantly higher in farmers' lung patients than in the other groups. there was no difference between controls and patients with bronchial asthma or non-allergic pulmonary diseases. on the other hand, in the group of patients with respiratory diseases of ...19806993041
environmental factors in allergic bronchopulmonary aspergillosis.many workers have suggested that exposure to an environment rich in aspergillus spores is aetiologically significant in allergic bronchopulmonary aspergillosis. this hypothesis has been investigated by means of an environmental survey in which eight urban dwelling patients with this disease and five atopic control patients took part. enquiries were made about contact with known sources of aspergillus fumigatus and air spora studies were conducted in the home. findings were similar for both group ...19806993043
antibodies against aspergillus fumigatus. i. standardization of the antigenic composition.three different stages were found during growth of aspergillus fumigatus as characterized by the changes in ph of the medium: (1) an acidic phase (phase i); (2) a second phase (phase ii) during which the ph rises till values around ph = 8.5; (3) a third phase (phase iii) during which the ph remains constant or drops slightly. during phase i a fast appearance of certain antigenic components was found that is ascribed to an active process of excretion. additional antigenic components appeared in t ...19806993368
antibodies against aspergillus fumigatus ii. identification and quantification by means of crossed immunoelectrophoresis.individual antigenic components of aspergillus fumigatus were identified by crossed immunoelectrophoresis (cie) using pooled rabbit hyperimmune antiserum. patients' sera with enhanced precipitins against a. fumigatus showed antibodies against identical antigenic components in cie. identification of the antigenic components by the intermediate gel technique showed high concentrations of antibodies against a small group of antigenic components. no differences were found in the individual antigenic ...19806993369
hydrolysis of cellulosic wastes by aspergillus fumigatus cellulase. 19806995286
degradation of melanin by aspergillus fumigatus.a strain of aspergillus fumigatus from composted coffee and garden wastes utilized natural deproteinized insect, banana, hair, octopus, and synthetic tyrosine and dopa melanins as sole sources of carbon. with a sucrose supplement, degradation was essentially complete after 50 days in czapek medium ph 6.5 at 30 degrees c. the catabolic rate differed for each substrate pigment, as did the molecular weight distribution of products accumulating in the medium. after incubation with l-[u-14c]melanin, ...19806996615
detection of candida antigenemia in human invasive candidiasis by a new solid phase radioimmunoassay.there is a continuing need to develop reliable non-invasive methods to aid in the early diagnosis of disseminatyed candidiasis. we report the development of a solid phase "sandwich" radioimmunoassay (ria) for the detection of soluble cytoplasmic protein antigens (spa) of candida albicans in patients with systemic candidiasis. spa were prepared by ultrasonic disruption of blastospores of c. albicans. anti-spa igg was covalently linked to a solid phase of polyacrylamide-like microspheres. standard ...19806997211
chromatographic purification and homogeneity of extracellular acid proteinase of aspergillus fumigatus.the extracellular acid proteinase from a. fumigatus was purified by employing several chromatographic procedures and a 164 fold purification with 22% yield was achieved. the homogeneity of the enzyme was confirmed by sds-polyacrylamide gel electrophoresis, immodiffusion and immunoelectrophoresis techniques.19806997232
[usefulness of counterimmunoelectrophoresis for serological diagnosis of pulmonary aspergillosis]. 19806997834
characterization of subspecies from a fungal fatty acid synthetase.fatty acid synthetase from the filamentous fungus, aspergillus fumigatus dissociates during gel filtration on sepharose 6b into two differently sized subspecies with mol. wt. approx. 1.5 x 10(6) and 8 x 10(5). after elution, they readily reform intact molecules, as determined by their enzymic activity (overall synthetase and 3-oxoreductase activities were measured), sedimentation coefficient and appearance in the electron microscope. synthetase was cross-linked with dimethyl suberimidate and the ...19806998497
changes in chemical composition of conidia of aspergillus fumigatus during maturation and germination. 19806999300
effect of five pesticides on the mycelial growth of some soil and pathogenic fungi.the effect of three doses (the lowest is the recommended dose in the field) of 5 pesticides namely: ceresan, orthocid (fungicides), vcs-438 (herbicide), and dursban and dipterex (insecticides) on the mycelial dry weight of aspergillus fumigatus. fusarium moniforme, pencillium italicum, and sclerotium ceprivorum was tested 2, 4, 6, 8 and 10 days after treatment. all the pesticides induced inhibition of mycelial growth of the test fungi. only vcs-438 at the low dose was stimulatory to f. monilifor ...19806999754
[physiological studies on thermophilic hydrocarbon-utilizing hyphae fungi (author's transl)].out of different substrates, thermophilic n-alkane-assimilating hyphae fungi were isolated and the strains of aspergillus fumigatus and mucor lusitanicus selected for physiological investigations. the growth curves, obtained in hydrocarbon and glucose at different temperatures, showed three phases: 1) initial growth phase, 2) linear growth phase, and 3) lysis phase. maximum dry matter and maximum protein content occurred within a range of 35-40 degrees c. during the entire time of growth the dna ...19806999773
prevalence of aspergillus fumigatus in free-living goshawks (accipiter gentilis atricapillus).during the fall migration of 1972 and 1973 unusually large numbers of goshawks (accipiter gentilis atricapillus) were counted at hawk ridge in duluth, minnesota. these birds were sampled for prevalence of fungi of the genus aspergillus. fungi of this genus were recovered from 26 of 49 birds (53%) in 1972 and 4 of 45 (7%) birds in 1973. aspergillosis was confirmed at necropsy in three wild goshawks in 1972, but none in 1973. the disease was further confirmed at necropsy in 8 of 12 (67%) goshawks ...19807001046
[liver abscess in chronic granulomatosis of childhood (author's transl)].a thirteen-year-old boy with an abscess of the liver and recurrent infections since infancy is described. aspergillus fumigatus could be isolated from this surgical treated abscess. polymorphonuclear leukocytes (pmn) and monocytes from the patient show a normal phagocytosis, whereas the oxygen metabolism and the intracellular killing of kastalase-positive microorganismus (staphylococcus aureus, candida albicans) are strikingly decreased. in contrast, the catalase-negative pneumococcus pneumoniae ...19807001145
[case of an allergic form of pulmonary aspergillosis]. 19807001390
extrinsic allergic alveolitis in a sawmill worker. a case report.extrinsic allergic alveolitis, with acute attacks of the disease, developed in a sawmill worker. the diagnosis was based on the history of exposure to moldy wood, on the symptoms and clinical signs, and on physiological and radiographic findings. a microbiological analysis of the ambient air of the patient's work environment showed that the air contained a considerable number of spores, both of fungi and actinomycetes. the most common fungal genus was aspergillus. thermoactinomyces vulgaris pred ...19807001618
allergic bronchopulmonary aspergillosis complicating cystic fibrosis in childhood.allergic bronchopulmonary aspergillosis, known to be associated with cystic fibrosis in older patients, occurred in 7 young atopic children with cystic fibrosis. the diagnosis was suggested by the onset of, or the increase in, asthmatic symptoms accompanied by major chest x-ray changes ranging from total collapse of a lung or lobe to extensive but changing areas of consolidation. each of the children had a blood eosinophilia, positive type i skin tests to aspergillus fumigatus, and reversible ai ...19807002053
prick testing for aspergillus. 19807002364
uncommon causes of peritonitis in patients undergoing peritoneal dialysis.in five patients, peritonitis caused by uncommon agents was acquired during continuous ambulatory peritoneal dialysis. three patients had yeast (two candida albicans, one c parapsilosis), one aspergillus fumigatus, and one nocardia asteroides infections. a review of the literature indicates that yeasts are the most common cause of peritoneal dialysis-associated fungal peritonitis; only one other possible case of aspergillus sp and no nocardia infections have been proved, to our knowledge. observ ...19817004371
standardization of allergen extracts by inhibition of rast, skin test, and chemical composition.five allergen extracts of dermatophagoides pteronyssinus, lolium perenne, alternaria tenuis, aspergillus fumigatus and cladosporium herbarum, obtained from four different manufacturers, were examined by inhibition of rast, content of protein and carbohydrate, contents of phosphorylcholine (pc) and tridacnin reactive components, and by skin test. inhibition of rast was used as a primary method for establishing allergenic potency and demonstrated wide variations for each preparation supplied by th ...19807004662
[multifocal fungal infections in patients coming to the laryngologists (author's transl)]. 19807005825
analysis of wall antigens of aspergillus fumigatus by two-dimensional immunoelectrophoresis.water-soluble and surface-located antigens from aspergillus fumigatus mycelium were analysed by two-dimensional electrophoresis (2d iep) with selected sera. a complex pattern was seen when a water-soluble fraction reacted with hyperimmune sera. significantly fewer precipitin peaks were seen when wall-located antigens were used in the 2d iep system; this made possible the detection of a small number of recurring antigen-antibody interactions when sera from patients with aspergilloma or allergic b ...19817007645
[sphenoidal aspergilloma]. 19807008316
the use of enzyme-linked immunosorbent assay (elisa) in the diagnosis of farmer's lung.enzyme-linked immunosorbent assay (elisa) was adapted to measure antibodies against aspergillus fumigatus, thermoactinomyces vulgaris and micropolyspora faeni. mycelial and culture fluid antigens were used separately to analyze antibodies in three study groups: farmer's lung patients, patients with bronchitis and healthy control persons. the mycelial antigen of a. fumigatus gave more positive results than the corresponding culture fluid antigen. the situation was reversed for the actinomycetes. ...19807008641
iga antibody against aspergillus fumigatus antigen in patients with allergic bronchopulmonary aspergillosis.iga antibodies against aspergillus fumigatus (af) antigen were measured by a radioimmunoassay in sera from patients with allergic bronchopulmonary aspergillosis (abpa) and compared with iga antibody levels in asthmatics who had positive prick tests to af and normal controls. the assay for iga antibody discriminated between both control groups and the abpa group at two serum dilutions (10(-2) and 10(-3)) but did not discriminate all individual abpa sera from the peak levels of antibody in the con ...19807008988
[primary, disseminated aspergillosis with signs of hilar lymphoadenopathy in chest x-ray (author's transl)]. 19807009943
the effect of calcium on protoplast release from species of aspergillus.the effect of various osmotica and lytic enzyme treatments, on the release of stable protoplasts from aspergillus niger and two strains of aspergillus fumigatus, has been investigated. most osmotica supported protoplast release at a concentration of 0.6 m except for cacl2 and ca(no3)2. supplementation of 0.6 m kcl with 0.2 m cacl2 enhanced release from both species, but depended upon strain of organism, concentration of osmoticum and enzyme system employed. more protoplasts were released at low ...19807010082
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