| low levels of mumps virus antigen induced interferon-alpha production in insulin-dependent diabetes. | the in vitro production of interferon-alpha was studied in cultures of peripheral blood mononuclear cells from children with insulin-dependent diabetes. significantly lower levels (p less than 0.01) of interferon (median 64 iu/ml) were found in mumps antigen stimulated cultures of iddm patients compared to control children (median 256 iu/ml) whereas no differences between groups were found in the amount of interferon induced by coxsackie b pool antigen or live sendai virus. | 1989 | 2561352 |
| the effect of cimetidine on survival of mice infected with herpes simplex virus type 2, murine encephalomyelitis virus and vesicular stomatitis virus infections. | the effect of cimetidine on survival was investigated in mice infected with herpes simplex virus type 2 (hsv-2), murine encephalomyelitis virus (gd-vii), and vesicular stomatitis virus (vsv). balb/c mice, 5 weeks of age, were injected intraperitoneally (i.p.) with 5.5 x 10(5) plaque-forming units (pfu) of virus/0.5 ml, and cimetidine (1 mg/0.5 ml) was administered simultaneously. the survival rates of 80% and 85% in the cimetidine groups were significantly greater than the 10% and 23% for the co ... | 1989 | 2561403 |
| [prospect of avian encephalomyelitis antibodies in breeding farms in chile]. | ten different layer and broiler breeding farms, located in the central part of the country were sampled to detect antibodies against the avian encephalomyelitis virus (aev). fifty embrionated eggs, per house, were examined using the embryo susceptibility test. samples of eggs from 21 houses of broiler breeder hens and 9 of breeder layers were collected from all the poultry farms studied. the antigen used in the embryo susceptibility test was prepared from the van roekel strain for aev. the resul ... | 1989 | 2561813 |
| [determination of antibody levels against coxsackie b virus type 2 and type 3 in sera from blood donors using the microneutralization test]. | the presence of antibodies and their titers against coxsackie b virus type 2 and type 3 (cbv-2 and cbv-3) have been investigated in 208 blood donor's sera obtained from blood bank of gülhane military medical academy. the seropositivity against coxsackie b virus type 2 and type 3 have been determined 59.1% (123/208) and 7.2% (15/208) respectively. these findings suggest that coxsackie b virus type 2 infections may be present more widely than coxsackie b virus type 3 infections in our country. | 1989 | 2562136 |
| serologic, virologic, and histopathologic observations of encephalomyocarditis virus infection in mummified and stillborn pigs. | stillborn and mummified swine fetuses from swine farms experiencing reproductive problems were investigated for evidence of infection with encephalomyocarditis (emc) virus by fetal serology, virus isolation, and histopathologic examination. fetal sera or thoracic fluids of 478 abnormal fetuses collected during january through december 1987 were tested for the presence of antibody specific to emc virus. of 478 samples tested, 175 (36.6%) had emc virus serum neutralizing antibody titers of 1:64 or ... | 1989 | 2562190 |
| use of in situ hybridization for the detection of foot-and-mouth disease virus in cell culture. | biotinylated complementary dna (cdna) and rna probes were prepared from a specific and highly conserved section of the foot-and-mouth disease virus (fmdv) genome coding for the rna-dependent rna polymerase. hybridization was conducted on fmdv-infected, bovine enterovirus (bev)-infected, and noninfected swine kidney cell cultures. the detection system utilized the enzyme system streptavidin-alkaline phosphatase, the substrate phosphate, and the chromogen nitroblue tetrazolium. intense cytoplasmic ... | 1989 | 2562224 |
| effect of measles, mumps, rubella vaccination on pattern of encephalitis in children. | 462 patients (269 males, 193 females, aged from 1 month to 16 years) with encephalitis were treated at the children's hospital, university of helsinki, over a 20-year period. the incidence of encephalitis was 8.3/100,000 child-years (range 19.8 in 1974 to 2.5 in 1985 and 1986). the organisms most commonly associated with encephalitis in children were mumps, measles, and varicella viruses, and mycoplasma pneumoniae. after the start of the nationwide measles, parotitis, and rubella (mpr) vaccinati ... | 1989 | 2563011 |
| conservative management of an echovirus 11 outbreak in a neonatal unit. | two babies in a neonatal unit presented on the same day with meningitis due to echovirus 11, which was thought to have been introduced by staff. at this time echovirus 11 was also isolated from the stools of eight other babies; five of them did not have signs of infection. no intervention was made except to emphasise the importance of handwashing. there was evidence of secondary spread to two babies who were both clinically well. the attack rate was twelve (29%) of forty-one babies exposed. seve ... | 1989 | 2564069 |
| chronic relapsing pericarditis and dilated cardiomyopathy: serological evidence of persistent enterovirus infection. | by means of two different igm-capture assays, enterovirus-specific igm responses were shown in 9 of 14 (64%) patients with chronic relapsing pericarditis. this finding suggests persistent enterovirus infection, particularly coxsackie b virus infection. igm responses persisted for at least 1 year and for up to 10 years after onset of symptoms. in contrast, patients with acute enterovirus infections, including acute pericarditis, had transient responses. among patients with acute pericarditis, the ... | 1989 | 2564902 |
| vp-1 antigen in chronic postviral fatigue syndrome. | | 1989 | 2565511 |
| occurrence and aetiology of acute respiratory diseases: results of a longterm surveillance programme. | totals of 58,661,000 acute respiratory disease (ard) cases, 1,376,651 bronchitis and pneumonia complications, and 93,042 deaths from influenza, bronchitis, pneumonia or chronic pulmonary affection were notified during 11 years of ard surveillance from 1975 to 1986. all ard seasons started with the first phase in september-december; this increase in morbidity was caused chiefly by adenoviruses, parainfluenza viruses, rhinoviruses and m. pneumoniae. second wave of ard morbidity occurring in januar ... | 1989 | 2565674 |
| conservative management of enterovirus infections in neonatal intensive care units. | | 1989 | 2566042 |
| enterovirus infection and cardiomyopathy in cameroon. | | 1989 | 2566755 |
| contamination of endoscopes used in aids patients. | contamination of twenty endoscopes used in patients with aids was assessed. the suction-biopsy, air, and water channels and the insertion tube were sampled after use, after washing in detergent, and after disinfection for 2 min in 2% alkaline glutaraldehyde. the polymerase chain reaction with southern blotting, cell cultures, and antigen immunoassay were used to detect human immunodeficiency virus (hiv). samples were also examined for cytomegalovirus, adenoviruses, enteroviruses, herpes simplex ... | 1989 | 2567880 |
| effect of viral infection of fc receptors and immunologically nonspecific receptors of blood polymorphonuclear leukocytes: an in vitro model. | i describe the effect on phagocytic activity of the migration of bovine blood polymorphonuclear leukocytes (pmnl) through collagen and a monolayer of pk-15 cells in vitro infected with rotaviruses (strain osu, rfc-17, sa-11), a picornavirus (strain svdv), bovine herpesvirus (bhv-1) and equine herpesvirus (ehv-1). the pmnl were examined before and after their migration as follows: (1) for ea rosette formation with sensitized sheep erythrocytes, (2) for phagocytic activity mediated through fc rece ... | 1989 | 2569805 |
| coxsackievirus-induced disease. cd4+ cells initiate both myocarditis and pancreatitis in dba/2 mice. | dba/2 male mice inoculated intraperitoneally with 1.8 x 10(5) plaque-forming units (pfu) coxsackievirus b-3 (cvb3) showed extensive inflammatory cell infiltration of the myocardium and acinar tissue of the pancreas in 7 days. selective depletion of t lymphocyte subpopulations indicated that cd4 cells were either completely or partially responsible for cell damage in both organs. other organs such as the liver were infected and contained virus titers equivalent to those seen in the heart and panc ... | 1989 | 2573284 |
| pancreatic d-cell disorder in coxsackievirus b4-induced diabetic mice. | pancreatic d-cell disorder was analyzed in coxsackievirus b4-induced diabetic mice employing molecular hybridization with a radiolabelled probe to quantitate somatostatin mrna, and specific immunoprecipitation to measure somatostatin synthesis and its release. many infected mice showed blood glucose lower than noninfected control animals at 72 h postinfection and 85% became hyperglycemic at 6-8 weeks postinfection. pancreatic somatostatin decreased by 24% and 43% at 72 h and 6 weeks postinfectio ... | 1989 | 2575548 |
| neutralizing monoclonal antibodies to theiler's murine encephalomyelitis viruses. | theiler's murine encephalomyelitis viruses (tmev) are serologically related picornaviruses which cause both enteric and neurological disease in mice. the biological activities of tmev vary between the two different tmev subgroups (to and gdvii) and with different passage histories of the same tmev strain (e.g., mouse brain-passed versus tissue culture-passed da strain of the to subgroup). we raised neutralizing monoclonal antibodies (mabs) against tissue culture-passed da and gdvii strains of tm ... | 1985 | 2578578 |
| a serological classification of bovine enteroviruses. | cross virus neutralization (vn), complement fixation (cf) and immunoprecipitation (ip) tests were employed to compare the seven currently recognized bovine enterovirus (bev) serotypes with seven serologically distinct strains previously isolated in great britain and two other bev from the united kingdom. based on criteria used to differentiate other human and animal picornavirus serotypes, it was discovered that bev types 1, 4, 5 and 6 were related to each other and could be included in a single ... | 1985 | 2578783 |
| role of sex and early interferon production in the susceptibility of mice to encephalomyocarditis virus. | adult female swiss mice showed a greater resistance to intraperitoneal (i.p.) infection with encephalomyocarditis virus (emcv) than male mice. this difference was not observed in weanling mice, in castrated adult mice or in adult mice injected intracerebrally. administration of antibody to mouse interferon alpha/beta enhanced the virulence of emcv for both sexes and no difference was then observed in susceptibility between male and female mice. six h after emcv infection, serum interferon titres ... | 1985 | 2580047 |
| antimyocarditic activity of the guanine derivative biolf-70 in a coxsackievirus b3 murine model. | prophylactic administration of a nontoxic dose of 9-[[2-benzyloxyl-1-(benzyloxymethyl)ethoxy]methyl]-6-chlo roguanine (biolf-70) to mice reduced the number of myocarditic lesions induced by coxsackievirus b3 (cvb3). biolf-70 exhibited minimal antiviral activity against cvb3 in hela cells and murine neonatal skin fibroblasts and minimally reduced cvb3 yields in heart tissues. the drug had no effect on serum anti-cvb3 neutralizing antibody titers and did not induce the production of interferon. fl ... | 1985 | 2580480 |
| the complete nucleotide sequence of coxsackievirus a21. | we have determined the complete nucleotide sequence of coxsackievirus a21 (cav-21), the first member of this enterovirus subgroup to be analysed in molecular detail. the sequence, which is 7401 nucleotides long, encodes an open reading frame of 2206 codons, preceded by a 5' non-coding region of 711 nucleotides and followed by a 3' non-coding region of 72 nucleotides plus a poly(a) tract. the most striking feature is the remarkable homology to the poliovirus (greater than 90% at the amino acid le ... | 1989 | 2584950 |
| steady-state infection by echovirus 6 associated with nonlytic viral rna and an unprocessed capsid polypeptide. | we established a human cell line which was persistently infected (pi) by the normally cytolytic echovirus 6. all of the cultured pi cells contained genome-size viral rna which was synthesized continuously and incorporated into virus particles. this steady-state infection has been maintained for more than 6 years. in contrast to rna of wild-type echovirus 6, the viral rna from pi cells was not lytic when transfected into uninfected, susceptible cells. the capsid polypeptides of the virus particle ... | 1989 | 2585604 |
| fatal outcome of echovirus 7 infection. | a 9-month-old boy died of a disseminated echovirus 7 (e7) infection with virus being cultured from his liver and spleen. his hepatic failure was complicated by candidiasis and marrow hypoplasia and aggravated by a possible immune deficiency. | 1989 | 2587948 |
| severe encephalitis associated with disseminated echovirus 22 infection. | severe encephalitis associated with disseminated echovirus 22 infection occurred in a previously healthy 5-month-old boy. echovirus 22 was diagnosed by a seroconversion both in serum and cerebrospinal fluid and by isolation of the virus from several stool samples. the child damaged severely and at the age of 8 1/2 months infantile spasms developed. | 1989 | 2587949 |
| [neurosonography of the brain in infectious toxicoses in infants]. | brain neurosonography was performed in 15 babies with toxicosis-+-linked nervous disorders. three types of the brain structure were established in the patients: 1, marked brain swelling in all patients; 2, concomitant perinatally induced brain structures' changes; 3, intracranial hemorrhage focuses resulting from the dic syndrome. the technique is useful for precise diagnosis and prognosis of the outcome of the neurotoxic coma. | 1989 | 2588907 |
| sewage pollution of marine waters: the risks of viral infection. | | 1989 | 2593901 |
| waterhouse-friderichsen syndrome caused by haemophilus influenzae type b in an immunocompetent young adult. | a previously healthy 19-year-old woman had a febrile illness with hypotension, progressive cyanosis, and an evolving petechial rash. despite aggressive therapy in the face of shock and disseminated intravascular coagulation, the patient suffered a cardiac arrest and could not be resuscitated. haemophilus influenzae type b was cultured from the blood and echovirus 30 from the cerebrospinal fluid post mortem. fulminant h influenzae type b infection in an immunocompetent adult is rare but should be ... | 1989 | 2595428 |
| fatal echovirus 7 infection during an outbreak in a special care baby unit. | a case of fatal echovirus 7 infection in a neonate which was probably acquired from the symptomatic mother and an outbreak of infection in a neonatal unit are described. the baby who died had extensive haemorrhagic necrosis of the brain, liver, adrenal glands and kidneys as well as disseminated intravascular coagulation. three other babies and one member of staff were found to be infected. no other babies died. human normal immunoglobulin was administered to all babies on the unit. | 1989 | 2600441 |
| [epidemic of aseptic meningitis with echovirus type 7 in gifu prefecture in 1986]. | during the period from april to november in 1986, an epidemic of aseptic meningitis occurred in gifu prefecture. epidemiological, virological and serological investigations were performed. cases involved ranged from 0 to 30 years of age, and 84.2% of them were under 7 years of age. seven types of enteroviruses were isolated from 136 of 190 cases (71.6%), and echovirus type 7 was recovered from 121 cases (88.9%). with the cross neutralizing test between the prototype and isolated strains in 1986 ... | 1989 | 2614095 |
| postviral syndrome--how can a diagnosis be made? a study of patients undergoing a monospot test. | eighty-nine of 150 patients having a monospot test filled out a questionnaire about their illness, and the general health questionnaire. they completed a follow-up questionnaire 6 months later. twelve (8%) had a positive monospot. twenty-eight of 83 serum samples tested (34%) were positive for vp1 enteroviral antigen. forty of the patients had a self limiting illness, 13 had a definite diagnosis (excepting glandular fever), 14 had a possible postviral syndrome, 10 had recurrent sore throats/flu, ... | 1989 | 2614761 |
| [non-poliomyelitis viruses in poland 1982-1987]. | diseases caused by non-polio enteroviruses (enp) are diagnosed virologically and serologically in 17 virology laboratories of the province sanitary-epidemiological stations and the department of virology, state institute of hygiene. the results of these investigations are available in the form of documentation including the type of the isolated virus, the result of serological investigation, the date of disease onset, the clinical diagnosis, the age and sex of the patient. these data were analyz ... | 1989 | 2616797 |
| an outbreak of acute flaccid paralysis in jamaica associated with echovirus type 22. | an outbreak of acute flaccid paralysis in jamaica in 1986 associated with echovirus type 22 is described. six patients aged 1 to 27 years developed acute onset of severe flaccid paralysis, with inability to walk. three cases had facial weakness, four required intensive care with assisted ventilation, and two died. echovirus type 22 was isolated from the stool of two patients who showed a significant increase in antibody titre. echovirus type 22 was also isolated from the stool of another patient ... | 1989 | 2621458 |
| [clinico-morphological changes in congenital carditis in children]. | the clinico-morphological alterations were analyzed in 6 deceased children with congenital carditis. morbid anatomy examination demonstrated that all the patients had marked alterations on the part of the heart while 4 cases manifested associated injuries to the heart and to the brain. in half of the children, the disease had run its course in combination with immunodeficiency. in 2 cases, there was an etiological relationship between congenital carditis and intrauterine enterovirus coxsackie in ... | 1989 | 2628903 |
| [role of viral reactivation in recurrent adenoiditis and tonsillitis in children]. | we have been made virus cultures, excepting ebv, in adenoid and palatine tonsil tissue come from adenotonsillectomy. they were practised to eighteen patients between 4 and 17 years old who suffered recurrent infections processes of these organs. the total was 22 specimens to estimate the reactivation function of latent virus in the etiology of this pathology. | 1989 | 2629934 |
| study of an outbreak of epidemic conjunctivitis in delhi in 1986. | an epidemic of acute conjunctivitis occurred in delhi during july-september 1986. the clinical presentation in total of 350 patients were characteristic of acute haemorrhagic conjunctivitis (ahc). bacterial cultures from conjunctival swabs were sterile in 85 out of 105 (80%) patients, virus cultures in vero and hela cells were negative in 30 patients. however, conjunctival scrapes from 20/30 (66.7%) patients showed specific cytoplasmic fluorescence with enterovirus 70 (ev 70) antiserum, suggesti ... | 1989 | 2632413 |
| bacterial and viral causes of acute diarrhoea in children in kuwait. | bacterial and viral causes of acute diarrhoea were studied prospectively for one year in 343 hospitalised young children in kuwait. in 288 (84%) patients, one or more pathogens were identified compared with 12 of 86 (13.9%) children admitted with diseases other than diarrhoea (p less than 0.01). forty-four (12.9%) of the patients were infected with two or more pathogens. viral agents detected in the stools were rotaviruses (40.2%), enteric adenoviruses (1.7%), and enteroviruses (1.5%). enterobac ... | 1989 | 2632640 |
| [computer model for prognosis of incidence of aseptic meningitis]. | a possibility of construction of a mathematical model for current prognosing of aseptic meningitis incidence both for 2-4 months in advance and for the whole year has been demonstrated. the initial information consisted of the data on prevalence of enteroviruses in water objects as from may. comparison of the actual and estimated incidence indicates that with the proposed model, up to 90.4% of the incidence of aseptic meningitis may be explained. | 1989 | 2633465 |
| role of the virology laboratory in diagnosis and management of patients with central nervous system disease. | a number of viruses cause acute central nervous system disease. the two major clinical presentations are aseptic meningitis and the less common meningoencephalitis. clinical virology laboratories are now more widely available than a decade ago; they can be operated on a modest scale and can be tailored to the needs of the patients they serve. most laboratories can provide diagnostic information on diseases caused by enteroviruses, herpesviruses, and human immunodeficiency virus. antiviral therap ... | 1989 | 2644021 |
| eruptions associated with respiratory and enteric viruses. | | 1989 | 2647268 |
| clinical and experimental aspects of viral myocarditis. | picornaviruses are frequently implicated as the etiological agents of acute myocarditis. this association is based historically on serological evidence of rising antibody titers to specific pathogens and more recently on identification of viral genomic material in endocardial biopsy specimens through in situ hybridization. only rarely is infectious virus isolated from either the patient or the heart during periods of maximum myocardial inflammation and injury. thus, despite a probable viral etio ... | 1989 | 2650861 |
| anti-kj: a new antibody associated with the syndrome of polymyositis and interstitial lung disease. | antibodies to aminoacyl-trna synthetases (anti-jo-1, anti-pl-7, anti-pl-12) have been found in the serum of some patients with polymyositis (pm). patients with these antibodies have an unusually high rate of interstitial lung disease (ild) in association with their pm. two patients (k.j. and b.t.) with severe ild and pm were found to have antibodies to a cytoplasmic antigen, but tests to determine whether the antigen was an aminoacyl-trna synthetase were negative, including tests of kj serum for ... | 1989 | 2661587 |
| immunomodulating therapy in experimental myocarditis. | | 1989 | 2665156 |
| [deficiencies in humoral immunity]. | b cell immune deficiencies are characterized by inadequate production of antibodies and/or low levels of one or more classes of immune globulins (igg, iga, igm) or igg sub-classes. they include: 1) severe deficiencies involving all the immune globulins (bruton agammaglobulinemia, variable hypogammaglobulinemia); 2) selective deficiencies in immune globulins (iga deficiency, igm deficiency, deficiencies in igg sub-classes, dysgammaglobulinemias); 3) transient infantile hypogammaglobulinemia; 4) b ... | 1989 | 2667425 |
| routine viral culture for pediatric respiratory specimens submitted for direct immunofluorescence testing. | from 1986 to 1987, 69 (25%) of 274 specimens from children with lower respiratory tract syndromes were positive for respiratory syncytial virus antigen by direct immunofluorescence assay (dfa). comprehensive viral culture was performed on all 205 dfa-negative specimens, and 72 specimens yielded viruses; 5 specimens yielded multiple agents. thus, 52% of specimens yielded a specific virus, supporting the routine use of viral culture. isolates from the dfa-negative specimens included respiratory sy ... | 1989 | 2671012 |
| [dilated cardiomyopathy--heart muscle disease of unknown origin or an autoimmune disease? new aspects of etiology, pathogenesis and clinical practice]. | recent evidence suggests that the most common form of idiopathic cardiomyopathy in our altitudes, the dilated cardiomyopathy (dcm), is a post-infectious autoimmune disease which is triggered by virus infections. in animal experiments, the development of the coxsackie virus b3 myocarditis to a congestive cardiac insufficiency resembling the clinical picture of dcm was demonstrated. in mice, species-dependent varying disease courses could be observed, which point to a genetically different behavio ... | 1989 | 2683430 |
| [significance of viruses of antigen complex of california encephalitis in pathology]. | a study was undertaken to examine 320 patients with seasonal fevers occurred in june to september. these included fever of unknown etiology, acute respiratory virus diseases, pneumonias, bronchitis, enterovirus diseases, and serous meningitis. serological tests revealed that in 20 (6.3%) of them the viruses of the complex in california encephalitis (tyagin's virus or antigenically related virus) contributed to the etiology of the disease. major clinical symptoms of the disease were defined in th ... | 1989 | 2685461 |
| [viral encephalitis]. | acute viral and other infectious causes of encephalitis usually produce fever, headache, stiff neck and alterations in consciousness, focal neurologic signs and seizures. a large number of viral and nonviral agents can cause encephalitis. arthropod-borne viruses peak in summer, the tick-borne infections occur in early summer, enterovirus infections in later summer and mumps in the winter and spring. | 1989 | 2696865 |
| antibody response of infants in tropics to five doses of oral polio vaccine. | sixty-two infants 1-12 months of age were administered five doses of trivalent oral polio vaccine (topv) at intervals of 4 weeks. the seroconversion achieved were 88.7, 93.5 and 96.5 per cent for type i, ii, and iii polioviruses. these seroconversion rates are significantly better when compared to seroconversion achieved after two and three doses of trivalent oral polio vaccine. factors which were considered in previous studies to be responsible for low seroconversion rates viz. interference by ... | 1989 | 2709485 |
| comparison of indirect and sandwich elisa for the identification of echo viruses. | an indirect enzyme linked immunosorbent assay (elisa) was standardized for the identification of echo viruses isolated in buffalo green monkey (bgm) kidney cell culture on inoculation of 113 sewage samples. comparable results were obtained with both indirect and sandwich elisa for the identification of echo viruses in respect of 15 out of 34 sewage samples which showed 75-100% cpe in bgm monolayers. | 1989 | 2723423 |
| characterization of a yac-1 mouse cell receptor for group b coxsackieviruses. | a receptor on yac-1 cells, a mouse t-lymphoma cell line, bound all six serotypes of the group b coxsackieviruses (cvb). in addition, the cells produced infectious virus. each of the cvb competed for the same receptor on yac-1 cells. cvb3 bound relatively slowly to yac-1 cells (k = 4 x 10(-11) min-1 cell-1), and there were only 500 attachment sites per cell. a rabbit antiserum prepared against the hela cell receptor protein rp-a specifically inhibited the binding of cvb1 and cvb3. a virus-recepto ... | 1989 | 2724420 |
| [echo-ii virus, the pathogen of the third outbreak of uveitis in children in kransnoyarsk, 1986]. | the third outbreak of an acute infection accompanied in 3 cases by inflammation of the uveal membrane (uveitis) was recorded among infants in krasnoyarsk in 1986. from the infants with the eye involvement as well as from those in contact with the former, 36 strains of echo-11 virus were isolated, and seroconversion to this virus was demonstrated. all the infants in contact with the eye patients were given gamma-globulin which seemed to limit the spread of the infection. the third outbreak of uve ... | 1989 | 2728407 |
| [virologic and morphologic study in sudden and unexpected death in children]. | the authors examined a group of 47 children who died at the age of 1-9 months, incl. 19 female and 28 male children. in 23 the death was described as sudden and in 24 as unexpected. from 19 dead children a virus was isolated: in 14 one or two serotypes of enterovirus were found, in two the virus of parainfluenza 3 in two adenoviruses (type 2, 7). a total of 46 viral strains were isolated. by immunofluorescence in tissues antigens of the parainfluenza virus 3 and adenovirus were detected. immune ... | 1989 | 2731279 |
| an outbreak of an enterovirus-like illness at a community wading pool: implications for public health inspection programs. | in june 1987, following an outbreak of an illness among children participating in a swim class, investigation revealed that 26 children who had swum in the outdoor wading pool were more likely to be ill than those who had not (or 12.1, 95% ci = 2.9, 74.2). the pool chlorination system was operating improperly prior to onset of illness and chlorine levels were at or very near zero. this report emphasizes the need for operators and inspectors to give special attention to disinfection of wading poo ... | 1989 | 2735481 |
| detecting inhibition of coxsackievirus replication by measuring dna synthesis--effect of proteinase inhibitors. | the replication of picornaviruses can be monitored by microscopic examination of the cytopathogenic effect. we found that measuring cellular dna synthesis gave a more objective and reliable estimate of the viral effect on the cells. furthermore, by simultaneously measuring dna synthesis in uninfected cells, we obtained a sensitive indication of whether the agents tested influenced cellular activity. the method was employed to investigate the effect of various proteinase inhibitors on the replica ... | 1989 | 2736105 |
| [the role of coxsackie virus infection in the development of type 1 diabetes mellitus and its effect on the postinitial course of the disease in childhood]. | the authors present the results of a three-year investigation of coxsackie viruses in children diabetics. in paired serum samples of 66 newly diagnosed patients of virus neutralizing antibodies against eight types of coxsackie viruses were estimated. concurrently specific antibodies of class igm were assessed and compared with a control group of 218 healthy children. infection with coxsackie viruses, in particular types b2, b4 and b5, were found in 38 diabetics (in 57.6%) as compared with 6% in ... | 1989 | 2752452 |
| duration and patterns of transmission of theiler's mouse encephalomyelitis virus infection. | the duration and patterns of theiler's murine encephalomyelitis virus (tmev) transmission were studied in eight index mice inoculated orally. transmission was monitored by testing for seroconversion to tmev in sentinel mice in direct contact with index mice and in other sentinel mice in contact with bedding soiled by index mice. for the first 14 weeks after inoculation, two contact sentinels were housed with each index mouse for 1 week, then replaced with two new sentinels. for the remaining 16 ... | 1989 | 2761233 |
| rapid development of renal lesions in diabetic dba mice infected with the d-variant of encephalomyocarditis virus (emc-d). | marked hyperglycaemia and renal lesions developed rapidly in dba mice infected with 10 plaque-forming units of the d-variant of encephalomyocarditis virus (emc-d). renal alterations were demonstrated in the glomeruli, tubular epithelium and small vessels 2 months after infection. glomerular changes were characterized by mesangial thickening due to an increase of basement membrane-like material in the mesangial matrix. nodular glomerular lesions were commonly observed 3 months after infection, wh ... | 1989 | 2765387 |
| characteristics of lymphocytes cultured from murine viral myocarditis specimens: a preliminary and technical report. | long-term culture of lymphocytes from myocardial biopsy specimens has been reported. to test the usefulness of this technique in experimental models of murine myocarditis, the culture and characterization of lymphocytes from mice infected with encephalomyocarditis virus or coxsackievirus b3 were studied. lymphocytes were successfully cultured from three hearts of encephalomyocarditis virus-infected mice in interleukin 2-containing culture. after approximately 10 days, lymphocytes migrated out of ... | 1989 | 2768726 |
| characterization of a non-structural 147-kda precursor polypeptide of echovirus 33 and its immunogenicity in man. | zinc chloride (0.9 mm)--an inhibitor of the processing of the initial polypeptides of picornaviridae--was used to accumulate large precursors of echovirus 33 (ev33) and notably two proteins of 147- and 97-kda. polyclonal hyperimmune sera were raised in mice against these polypeptides and assayed by immunoblotting against ev33-infected cells blocked or not with zncl2, showing that protein 147--2abc3abcd according to the l434 convention--can be considered to be the precursor of the two non-structu ... | 1989 | 2772414 |
| an enzyme-linked immunosorbent assay (elisa) for the detection and quantification of antibodies against swine vesicular disease virus (svdv). | a liquid phase blocking sandwich elisa has been compared with virus neutralisation for testing pig sera for antibodies against swine vesicular disease (svd) virus. highest infectivity titre of svd virus was obtained using a multiplicity of infection of 30 pfu/cell and harvesting after 21 h. titres obtained for 300 clinically normal animals were assessed by elisa and 89% were found to be 1/6 or less. results were skewed and spread up to 1/45. comparison of known positive sera resulted in a correl ... | 1989 | 2778030 |
| effect of the administration of oral poliovirus vaccine on infantile diarrhoea mortality. | infectious diarrhoea is one leading cause of infantile mortality in developing countries. rotaviruses and other enteroviruses have an important role in diarrhoea but, currently, there are no vaccines available to immunize infants. the use of oral poliovirus vaccine (opv) over two decades has shown that, besides protecting effectively against poliomyelitis, it can interfere with intestinal infection by other enteroviruses. this interference by the vaccine viruses could explain two epidemiological ... | 1989 | 2781855 |
| incidence of coxsackie virus infection in patients with dilated cardiomyopathy. | a total of 127 patients, divided into two groups, were examined; one was made up by patients with dilated cardiomyopathy (d-cmp) (n: 32), the other one by patients with myocarditis and pericarditis ("other cardiopathies") (n: 95). a control group of healthy subjects (n: 278) consisted of blood donors. infection with coxsackie viruses (b1, b2, b3, b4, b5, b6, a7, a9) was followed up serologically using virus-neutralizing antibodies and/or igm class virus-specific antibodies. actual coxsackie viru ... | 1989 | 2788555 |
| [immunomodulation mechanisms in the anti-tumor effect of the echo-7 enterovirus]. | it has been established that the complex systemic and local administration of human echo-7 virus exerts an inhibitory effect on the growth of mx-17 tumour in the balb/c mice without the signs of viral oncolysis. the antitumour effect is due to a modulation of the antitumour immunity (immunological regression) against a background of the viral immunogenesis. | 1989 | 2791955 |
| diarrhoea associated with ceftriaxone and its implications in paediatric patients. | we prospectively studied 50 paediatric patients (3 months-12 years) who received 50 mg/kg ceftriaxone, i.v. over 20 min every 12 h for 3-14 days. diarrhoea, as defined by four bowel movements/day for two consecutive days, occurred in 34% (17) of 50 patients. an additional two patients with diarrhoea were excluded due to the presence of enteroviruses. diarrhoea occurred after 2-14 (8.7 +/- 3.2) doses and persisted for 2-8 (3.1 +/- 1.7) days. the mean number of bowel movements was 1.2/days before ... | 1989 | 2793955 |
| outbreak of poliomyelitis-like paralysis associated with enterovirus 71. | in the summer of 1987 five children were seen at the children's hospital of philadelphia because of acute onset of flaccid paralysis of an arm or leg(s). although there were documented exposures to oral poliovirus vaccine and coxsackievirus b3 in some of the cases, the clinical, epidemiologic and laboratory findings indicate that enterovirus 71 was the common etiologic agent for this unusual outbreak of poliomyelitis-like paralysis. of the five children three recovered completely; the other two ... | 1989 | 2797956 |
| detection of parvovirus in a patient with "reactive arthritis" by in situ hybridization. | we used in situ hybridization to search for the presence of viruses in synovial fluid cell preparations obtained from patients with various forms of knee arthropathies. one patient, presenting with acute reactive arthritis, was found to replicate parvovirus dna in cells from synovial fluid whereas six other patients with various other forms of arthritis were negative for parvovirus infection. five patients with osteoarthritis, constituting a control group in which an infectious etiology would no ... | 1989 | 2805616 |
| a ten-year experience in control of poliomyelitis through a combination of live and killed vaccines in two developing areas. | we describe a successful program of poliomyelitis control using a combination of killed and live polio vaccines over a 10-year period in two developing areas, the west bank and gaza, adjacent to a relatively developed country, israel. during the 1970s, immunization using live trivalent oral polio vaccine (opv) in these areas covered more than 90 percent of the infant population. nevertheless, the incidence of paralytic polio continued to be high, with many cases occurring in fully or partially i ... | 1989 | 2817193 |
| investigations of an enteric infection of cockatoos caused by an enterovirus-like agent. | an enteric infection in cockatoos associated with a 30 nm diameter enterovirus-like agent seen in faeces and intestinal epithelial cells is described. the disease is characterised by intractable, profuse, mucoid diarrhoea, weight loss, dehydration and death. lesions in the intestine consist of villous atrophy, villous fusion, enterocyte hyperplasia and, in some cases, chronic inflammation. affected birds so far examined have concurrent psittacine beak and feather disease. | 1989 | 2818359 |
| in situ detection of enteroviral genomes in myocardial cells by nucleic acid hybridization: an approach to the diagnosis of viral heart disease. | we have developed an in situ hybridization assay capable of detecting enteroviral rna in myocardial cells, using molecularly cloned coxsackievirus b3 cdna as a diagnostic probe. because of the high degree of nucleic acid sequence homology among the numerous enteroviral serotypes, including the group a and b coxsackieviruses and the echoviruses, detection of these various agents commonly implicated in human viral heart disease is possible in a single hybridization assay. we demonstrate the consid ... | 1987 | 2819870 |
| triple plaque purified strains of duck hepatitis virus and their potential as vaccines. | individual triple plaque purified strains of attenuated duck hepatitis virus can protect ducklings against virulent challenge with the virus but they are as genetically unstable as their parent vaccine strains and are transmissible by direct contact. the use of rapid passage is advocated as a method to improve these parameters. | 1987 | 2820008 |
| antiviral activity of naphthoquinones. i. lapachol derivatives against enteroviruses. | | 1987 | 2820010 |
| [acute hemorrhagic conjunctivitis, in cuiabá, mato grosso, brazil 1983]. | | 1987 | 2820016 |
| myocarditis and dilated cardiomyopathy: different stages of the same disease? | | 1988 | 2820574 |
| immunopathologic basis of myocardial injury. | | 1988 | 2820575 |
| prodromal immune manifestations in emc-m virus induced diabetes: islet bound and circulating antibodies, and changes in lymphocyte subsets. | the thymus-dependence of the encephalomyocarditis (emc-m) virus induced diabetes has been demonstrated in comparative studies of normal and immunodeficient mice. since the lymphocytic infiltration in the islets of langerhans is modest during the virus infection, we have looked for possible indications of humoral immune mechanisms. using fluorescence microscopy the presence of immunoglobulins in the islets could be shown 3 days after emc-m-virus inoculation, gradually disappearing about day 14. t ... | 1987 | 2820645 |
| neonatal epidemic coxsackie b5 meningitis. | | 1987 | 2820658 |
| multiple and persistent viral infections in a patient with bare lymphocyte syndrome. | | 1987 | 2821129 |
| enterovirus replication in porcine ileal explants. | organ explants of porcine ileum were cultured in different media for up to 48 h. tissue preservation was evaluated by light microscopy and by transmission and scanning electron microscopy. cellular structure was well maintained after incubation for 48 h in cmrl-1066 supplemented with insulin and cortisone. explants of absorptive or lymphoid tissue from young or adult pigs were incubated with either coxsackievirus b5 (which is infectious for swine) or human poliovirus type 1 (which served as a co ... | 1987 | 2821187 |
| [is dilated cardiomyopathy an autoimmune disease?]. | | 1987 | 2821316 |
| [morphofunctional changes in a culture of pancreatic islet cells from newborn piglets as affected by coxsackie b3 viruses]. | the direct influence of coxsackie virus b-3 and its selected variants abent+ and abent- on the function and structure of neonatal pig pancreatic islet cells in monolayer cultures was studied. the viruses under study possessed cytopathogenic activity and were capable of reproduction. the abent- variant of coxsackie virus b-3 possessed the most pronounced cytopathogenic activity. direct correlation between virus reproduction and insulin concentration in a culture medium was established. | 1987 | 2821534 |
| the antibody response in theiler's virus infection: new perspectives on multiple sclerosis. | | 1987 | 2821576 |
| insulin-dependent (juvenile-onset, type 1) diabetes mellitus coxsackie b viruses revisited. | | 1987 | 2821577 |
| [serologic study of the occurrence of epizootic viral diarrhea in swine in switzerland]. | | 1987 | 2821613 |
| [the behavior of viruses in conventional and 2-step anaerobic-mesophilic stabilization of clarified sludge with pre- and postpasteurization]. | in a pilot-plant of semi-technical scale for the digestion of sewage-sludge with two single operated reactors and five coupled reactors investigations about the influence of anaerobic mesophilic sludge digestion and pasteurisation of raw and digested sludge on viruses were carried out. following test-viruses were used: bovine enterovirus ecbo strain lcr-4, bovine parvovirus strain "haden" and reovirus type i. in the case of a daily addition of virus-contaminated raw sludge both ecbo-virus lcr 4 ... | 1987 | 2821710 |
| replication of avian encephalomyelitis virus in chick embryo neuroglial cell cultures. brief report. | avian encephalomyelitis virus replicated to relatively high titres in chick embryo neuroglial cell cultures, as measured by the indirect fluorescent antibody test, but induced few cytopathic effects. this cell system should provide a good source of virus for serological tests and vaccines. | 1987 | 2821966 |
| evidence for the role of a human intestinal adenovirus in the pathogenesis of coeliac disease. | we previously noted a region of amino acid sequence homology between a-gliadin, a major alpha-gliadin component known to activate coeliac disease, and the early region e1b protein of human adenovirus serotype 12 (ad12), an adenovirus isolated from the human intestinal tract. in the present study sera from coeliac disease patients from the united kingdom and the united states were assayed for neutralising antibody to ad12 as evidence of past exposure to that virus and for antibody to synthetic pe ... | 1987 | 2822550 |
| the histopathology of enterovirus infections of new-born mice. | for 32 years we have provided a routine histopathological service on animals for the regional virus laboratory, ruchill hospital, glasgow. this article concerns mainly experimental coxsackievirus a, coxsackievirus b and echovirus infections in new-born mice. in addition to previously reported findings, we have sometimes observed degenerative and inflammatory changes in neurons related to the gasserian, posterior root or autonomic ganglia. not all deposits of brown fat are simultaneously the seat ... | 1987 | 2822811 |
| a novel presentation of coxsackie b2 virus infection during pregnancy. | a 39-year-old woman presented at 34 weeks gestation with a febrile illness. this initially settled but recurred 6 days later, when uncontrolled vaginal bleeding necessitated caesarian section. at operation, vesicles were seen on the mother's hyperaemic uterus. the infant developed tachypnoea, bradycardia, cerebral irritation, an enlarged liver and spleen, intravascular coagulation and a transient rash. mother and child made a full recovery. coxsackie b2 virus was isolated from the infant and ris ... | 1987 | 2822813 |
| coxsackie b2 virus causing simultaneous hand, foot and mouth disease and encephalitis. | | 1987 | 2822814 |
| paralytic poliomyelitis and non-polio enteroviruses in saudi arabia. | | 1987 | 2822943 |
| pathogenesis and preventive and therapeutic trials in an animal model of dilated cardiomyopathy induced by a virus. | an animal model of dilated cardiomyopathy following encephalomyocarditis (emc) virus has been developed. virus was isolated from mouse hearts and viral antigens were detected in the myocardium until the second week of infection, but neither was found thereafter. differences were found among different strains of mice in the frequency of occurrence and severity of myocarditis, and even in the character of the pathologic lesions. thus, genetic factors may play an important role in the pathogenesis. ... | 1987 | 2822976 |
| [various aspects of pathogenesis, clinical course and treatment of myocarditis. (ii)]. | | 1987 | 2822991 |
| clinical manifestations of acute coxsackie-b viral myocarditis and pericarditis with a special reference to serum enzyme patterns and long-term prognosis. | | 1987 | 2822999 |
| [antiviral immunity in multiple sclerosis and the use of live enterovirus vaccines]. | | 1987 | 2823392 |
| in situ immune autoradiographic identification of cells in heart tissues of mice with coxsackievirus b3-induced myocarditis. | in adolescent cd-1 male mice inoculated with a myocarditic coxsackievirus b3 (cvb3m) acute focal lesions containing necrotic myocytes, infiltrating mononuclear cells, and fibroblasts develop. with the use of an in situ immune autoradiographic method with rat monoclonal antibodies (mab) and an 35s-labeled antibody, viral antigens were detected outside of lesions. macrophages, t lymphocytes, and natural killer (nk) cells were identified within myocarditic lesions during the acute phase of the dise ... | 1987 | 2823612 |
| inactivation of animal viruses during sewage sludge treatment. | using a previously developed filter adsorption technique, the inactivation of a human rotavirus, a coxsackievirus b5, and a bovine parvovirus was monitored during sludge treatment processes. during conventional anaerobic mesophilic digestion at 35 to 36 degrees c, only minor inactivation of all three viruses occurred. the k' values measured were 0.314 log10 unit/day for rotavirus, 0.475 log10 unit/day for coxsackievirus b5, and 0.944 log10 unit/day for parvovirus. however, anaerobic thermophilic ... | 1987 | 2823708 |
| possible mechanisms in the pathogenesis of virus-induced diabetes mellitus. | insulin-dependent diabetes mellitus results from destruction of pancreatic beta cells. viruses and autoimmunity have been implicated as possible causes of beta cell destruction in genetically predisposed individuals. the evidence for viruses comes largely from experiments in animals, but several studies in humans point to viruses as triggers in the pathogenesis of diabetes in some cases. in animal models, at least 4 different possible mechanisms for virus-induced diabetes have been proposed. the ... | 1987 | 2824113 |
| cardiac myosin and autoimmune myocarditis. | infection with type 3 of the group b coxsackieviruses (cb3) sometimes leads to the development of myocarditis in humans. circumstantial evidence in the form of heart-reactive antibodies in these cases of human myocarditis suggests that the later phases of the disease may be due to autoimmunization. since human myocarditis is a relatively rare sequel to infection with cb3 virus, we propose that it reflects a genetic predisposition in some individuals. to investigate this possibility we establishe ... | 1987 | 2824143 |
| impaired cytokine response in male icr swiss mice after infection with d variant of encephalomyocarditis virus. | the basis for the resistance of the female and the susceptibility of the male icr swiss mouse to the diabetogenicity of the d variant of encephalomyocarditis virus (emcv-d) is unknown. this pattern of disease resistance and susceptibility can be reversed if females are treated with testosterone and males are treated with estrogen before virus infection. as a possible explanation for this sex difference in disease development, differences in early antiviral host responses were explored. cellular ... | 1987 | 2824259 |