| morphogenetic development of the area octavolateralis in the cichlid fish oreochromis mossambicus. | in the cichlid fish oreochromis mossambicus the area octavolateralis in the brain stem was studied using histological techniques and labelling of afferents from the inner ear and the lateral line system with horseradish peroxidase (hrp). adult fish and fish larvae aged 2, 4, 7, 10, and 15 days post hatching were investigated in order to analyze the ontogenetic development of these sensory systems during the critical phase of maturation from relatively immobile stages to actively swimming larvae. ... | 1991 | 1802932 |
| [in the dying cell secretory protein diffuses through the membranes into the cytosol]. | anti-horseradish peroxidase igg (a-hrp) secreting hybridoma lymphoblasts grown subcutaneously in recipient mice have been studied light and electron microscopically 30-120 min following capitation of the animals. conventional hrp-dab immunocytochemical staining was performed for demonstration of a-hrp which in the living cells was restricted to the rough endoplasmic reticulum, the perinuclear cisterns, the golgi apparatus and some microvesicles. 30 min after death in a number of the cells a-hrp ... | 1991 | 1801671 |
| measurement of fibrinogen adsorption from blood plasma using 125i-fibrinogen and a direct elisa technique. | a direct enzyme-linked immunosorbent assay (elisa), using a polyclonal anti-fibrinogen conjugated to horseradish peroxidase, was used to detect fibrinogen adsorption from blood plasma to ten different materials. adsorption was also measured with [125i]-fibrinogen. the materials studied included glass, biomer, immulon i, and a series of hydroxyethylmethacrylate (hema) and ethylmethacrylate (ema) co-polymers. for all the materials studied, the results from the elisa technique closely paralleled th ... | 1991 | 1786243 |
| suprachiasmatic nucleus and retinohypothalamic projections in moles. | the suprachiasmatic nucleus of the hypothalamus (scn) and the retinohypothalamic projections were identified in one species of old-world moles, all of whom are blind as a result of natural loss of vision. a cyto-architectonic study revealed that the scn is well developed, even though other visual nuclei in the dorsal thalamus and the midbrain are not. an immunohistochemical study showed that vasoactive intestinal polypeptide (vip)-like immunoreactive cell bodies and fibers were distributed in th ... | 1991 | 1764636 |
| tissue specific expression of the drosophila adh gene: a comparison of in situ hybridization and immunocytochemistry. | the tissue specific patterns for drosophila melanogaster alcohol dehydrogenase (adh) mrna and protein expression were determined using in situ hybridization and immunocytochemical techniques. alcohol dehydrogenase mrnas were localized in thin sections of frozen tissue using the hybridization of single stranded rna probes. alcohol dehydrogenase protein was identified in frozen tissue samples using adh antisera, a biotinylated secondary antibody, and streptavidin conjugated to horseradish peroxida ... | 1991 | 1756967 |
| retinal projections in the freshwater butterfly fish, pantodon buchholzi (osteoglossoidei). i. cytoarchitectonic analysis and primary visual pathways. | the freshwater butterfly fish, pantodon buchholzi, is a member of the most primitive radiation of teleosts. the retinofugal projections were studied in this fish with autoradiographic and horseradish peroxidase (hrp) methods, and the cytoarchitecture of the retinorecipient regions in the diencephalon and pretectum was analyzed with bodian-, cresylecht-violet- and acetylcholinesterase-reacted sections. the rostral diencephalon of pantodon contains a large retinorecipient nucleus, not previously i ... | 1991 | 1742599 |
| topographic distribution of the neurons of the central complex (centre médian-parafascicular complex) and of other thalamic neurons projecting to the striatum in macaques. | the distribution of the neurons of the central complex (or "centre médian-parafascicular complex") and of other thalamic regions projecting to the striatum was studied using a cartographic technique based on ventricular landmarks. the brain of a macaque was used as a reference for the cytoarchitectonic study of the complex. three parts were isolated: the pars parafascicularis (or medial part), the pars media (or middle part) and the pars paralateralis (or lateral part). wheat germ agglutinin con ... | 1991 | 1722293 |
| [tetramethylbenzidine--a chromogenic substrate for peroxidase in enzyme immunoassay]. | 3,3' 5,5'-tetramethylbenzidine (tmb) is a high sensitive chromogenic substrate for horseradish-peroxidase as a marker enzyme. in an enzyme immunoassay (eia) for alpha-1-fetoprotein and in an rapid eia for myoglobin it reveals higher sensitivity compared to o-phenylenediamine, the increase depends on reaction time. the optimal peroxide concentration depends on reaction time of enzyme chosen in different assays. tmb used for histochemistry is also suitable for eia if hydrochloric acid is used as s ... | 1991 | 1712522 |
| partition of free and monoclonal-antibody-bound horseradish peroxidase in a two-phase aqueous polymer system--novel procedure for the determination of the apparent binding constant of monoclonal antibody to horseradish peroxidase. | the principle that the antigen and the antibody prefer different phases in an aqueous two-phase system is the analytical basis of the work presented here. the antigen horseradish peroxidase, which is bound to a monoclonal antibody (mab), is separated from free ag in an aqueous phase system (polyethylene glycol (peg)/dextran) as a function of the concentration of mab. the plot of the partition coefficient kappa of horseradish peroxidase versus the concentration of mab yields a sigmoidal curve sim ... | 1991 | 1710876 |
| the origins of supraspinal projections to lumbosacral and cervical levels of the spinal cord in the gray short-tailed brazilian opossum, monodelphis domestica. | retrograde tracing techniques were used to identify supraspinal neurons that project to sacral, lumbar and cervical levels of the spinal cord in the gray short-tailed brazilian opossum, monodelphis domestica. injections of fast blue, true blue or wheat germ agglutinin conjugated to horseradish peroxidase into the sacral or lumbar cord labeled neurons in hypothalamic and brainstem nuclei reported to innervate the same levels in other mammals. injections at cervical levels produced extensive label ... | 1991 | 1684917 |
| sequence and tissue-specific expression of a putative peroxidase gene from wheat (triticum aestivum l.). | we have used a cdna clone encoding a pathogen-induced putative wheat peroxidase to screen a genomic library of wheat (triticum aestivum l. cv. cheyenne) and isolated one positive clone, lambda pox1. sequence analysis revealed that this clone contains a gene encoding a putative peroxidase with a calculated pi of 8.1 which exhibits 58% and 83% sequence identity to the amino acid sequence of the turnip (brassica rapa) peroxidase and a pathogen-induced putative wheat peroxidase, respectively. the tw ... | 1991 | 1653627 |
| [interaction between beta-endorphin, steroids and peptide hormones in fibrocystic lesions of the female breast]. | from preclimacteric women (n = 10, 45-50 years of age) with gross cystic breast disease, levels of beta-endorphin, estradiol, progesterone, luteinizing hormone, follicle-stimulating hormone, thyroid-stimulating hormone, cortisol and prolactin were assayed radiochemically in the breast cyst fluid and in plasma. the beta-endorphin concentration (fmol/ml) was increased more than fourfold in the breast cyst fluid (17.6 +/- 4.6 sem) than in plasma (4.2 +/- 0.5 sem). in the breast cyst fluid, estradio ... | 1991 | 1648846 |
| the use of tetramethylbenzidine for solid phase immunoassays. | a rapid, sensitive, solid-phase immunoassay, using horseradish peroxidase and 3,3',5,5'-tetramethylbenzidine, was found to be more sensitive and the colour developed was more stable compared with hrpo using 4-chloro-1-naphthol or diaminobenzidine. assay sensitivity was equal to or greater than that obtained with the alkaline phosphatase reaction. the essential step was to incubate filters in 1% dextran sulphate for 10 min in a ph 5 (10 mm citrate-edta) buffer before reacting with tmb (0.05 mg/ml ... | 1990 | 2273262 |
| a comparative study of three methods for the visualization of von willebrand factor (vwf) multimers. | this study was performed to compare three visualization methods for the detection of vwf multimers: autoradiography (125i), electroblot with a horseradish peroxidase system (blot-hrp), and an avidin-biotin peroxidase system (av-bio). each method was evaluated according to: 1) ability to visually detect bands and subbands thereby identifying von willebrand's disease (vwd) subtypes and normals, 2) reagent availability, 3) cost, and 4) time requirements. additionally, resolvability was evaluated ut ... | 1990 | 2084942 |
| formation of 8-methylguanine as a result of dna alkylation by methyl radicals generated during horseradish peroxidase-catalyzed oxidation of methylhydrazine. | methylhydrazine oxidation promoted by horseradish peroxidase-h2o2 or ferricyanide led to the generation of high yields of methyl radicals and to the formation of 7-methylguanine and 8-methylguanine upon interaction with calf thymus dna. methyl radicals were identified by spin-trapping experiments with alpha-(4-pyridyl-1-oxide)-n-tert-butyl nitrone and tert-nitrosobutane. the methylated guanine products were identified in the neutral hydrolysates of treated dna by high pressure liquid chromatogra ... | 1990 | 2176204 |
| quantitative study of the tectally projecting retinal ganglion cells in the adult frog: i. the size of the contralateral and ipsilateral projections. | the proportion of ganglion cells connected to the several central targets of the retinal projection varies in different species. in the frog, the retinotectal projection is clearly the largest branch of the optic pathway and the relative size of the tectally projecting population can be expected to be correspondingly great. however, there have been no studies aimed at quantifying the size of this population and at partitioning its contralateral and ipsilateral components. we injected the tectum ... | 1990 | 1707068 |
| gaba-like immunoreactivity in a population of locust intersegmental interneurones and their inputs. | intracellular labelling of locust intersegmental interneurones with lucifer yellow or horseradish peroxidase was carried out in combination with light and electron microscope immunocytochemistry by using an antibody raised against gamma amino butyric acid (gaba). fifteen percent (four out of 27) of intracellularly stained interneurones showed gaba-like immunoreactivity. this is in agreement with previous physiological observations that 20% of the interneurones in this population make inhibitory ... | 1990 | 1707067 |
| terminal arbors of individual, physiologically identified geniculocortical axons in the tree shrew's striate cortex. | we studied the terminal patterns of single, physiologically identified geniculocortical axons in the striate cortex of the tree shrew by using intracellular recording and labeling methods. axons were classified by their response to the onset (on-center) or offset (off-center) of a light stimulus presented to the ipsilateral or contralateral eye. then, we attempted to penetrate each axon for labeling with horseradish peroxidase. we recovered 23 axons and studied 16 of these in detail. light micro ... | 1990 | 1702114 |
| sublaminar organization within layer vi of the striate cortex in galago. | in this study we examined the organization of projections from the striate cortex to the dorsal lateral geniculate (gl) and pulvinar (pul) nuclei in the prosimian galago by using retrograde transport methods. injections of wheat germ agglutinin-conjugated horseradish peroxidase (wga-hrp) into the pul labeled two bands of cells in the striate cortex: the first consisted of large pyramidal cells in the upper half of layer v; the second consisted of small and medium-size pyramidal cells located in ... | 1990 | 1705271 |
| sexual dimorphisms in the vocal control system of a teleost fish: morphology of physiologically identified neurons. | in one species of vocalizing (sonic) fish, the midshipman (porichthys notatus), there are two classes of sexually mature males--types i and ii--distinguished by a number of traits including body size, gonad size, and reproductive tactic. the larger type-i males (unlike type-ii males and females) build nests, guard eggs, and generate several types of vocalizations. sound production by type-i males is paralleled by a proportionate increase of 600% in their sonic muscle mass. the motor volley from ... | 1990 | 2273398 |
| afferent connections of the optic tectum in channel catfish ictalurus punctatus. | horseradish peroxidase was injected unilaterally into the optic tectum of the channel catfish, ictalurus punctatus. the sources of tectal afferents were thereby revealed by retrogradely labeled neurons in various brain centers. retrogradely labeled cells were seen in both the ipsilateral and contralateral telencephalon. the superficial pretectal area was labeled on both sides of the brain. ipsilateral projections were also observed coming from the entopeduncular nucleus. both the anterior thalam ... | 1990 | 2078856 |
| a chromatic horizontal cell in the xenopus retina: intracellular staining and synaptic pharmacology. | 1. we identified a chromatic-type horizontal cell (c-cell) in the xenopus retina by intracellular dye injection with lucifer yellow or horseradish peroxidase (hrp). c-cells hyperpolarized in response to blue light and depolarized in response to red light. 2. in either photopic or mesopic states, moderate-intensity blue and red stimuli evoked responses that were inverted with respect to each other but of similar waveform and latency. in the presence of a bright green adapting field, the maximal v ... | 1990 | 1705962 |
| subdivisions and neuron types of the nucleus of the solitary tract that project to the parabrachial nucleus in the hamster. | the solitary nuclear complex (nst) consists of a number of subdivisions that differ in their cytoarchitectonic features as well as in the amounts of inputs they receive from lingual afferent axons. in this study horseradish peroxidase (hrp) was injected into the parabrachial nucleus (pbn) of the hamster to determine which of these subdivisions contain cells that project to the pons. in the rostral, gustatory division of the nst, the rostral central subdivision contains the greatest number of lab ... | 1990 | 2177063 |
| enantioselective oxidations of sulfides catalyzed by chloroperoxidase. | the chloroperoxidase-catalyzed and horseradish peroxidase catalyzed oxidations of sulfides by tert-butyl and other peroxides have been investigated. the former metal enzyme afforded the corresponding sulfoxides having r absolute configuration in up to 92% enantiomeric excess (ee), whereas the latter gave racemic products. the various factors that control the enantioselectivity of the oxygenation have been examined. | 1990 | 2271658 |
| cyanide detection using a substrate-regenerating, peroxidase-based biosensor. | an enzyme-based, dual working electrode system is described for the sensing of cyanide. horseradish peroxidase (hrp) is incorporated as the sensing element. a continuous monitoring of oxidative activity by the enzyme results through the generation and regeneration of substrates at the electrode surfaces. thus, hrp is oxidized by hydrogen peroxide generated from dissolved oxygen, at the primary electrode, and then reduced through the secondary electrode by mediated electron transfer using ferroce ... | 1990 | 2270864 |
| variability of light-evoked response pattern and morphological characterization of amacrine cells in goldfish retina. | amacrine cells of the goldfish retina were characterized electrophysiologically and subsequently labelled by intracellular injection of horseradish peroxidase. an attempt was made to broaden the electrophysiological classification of the cells. light-evoked sustained amacrine cell responses were divided into two subtypes depending on colour opponency. colour-coded responses (red/depolarizing and green/hyperpolarizing) were found to arise in amacrine cells possessing highly polarized dendritic fi ... | 1990 | 2262590 |
| reconstructions of efferent fibers in the postnatal hamster cochlea. | it is well known that adult-like physiological functioning of the mammalian postnatal cochlea occurs coincidentally with the presence of efferent synapses on outer hair cells (ohcs). this study described the cochlear innervation patterns of thick efferent fibers traveling in the vestibular nerve in postnatal hamsters ranging in age from day zero to day 10. at least three kinds of efferent fibers were labeled via an in vitro horseradish peroxidase (hrp) technique: varicose, thin efferents; nonvar ... | 1990 | 2292493 |
| mechanism of peroxidative activation of the bladder carcinogen 2-amino-4-(5-nitro-2-furyl)-thiazole (anft): comparison with benzidine. | the mechanism of activation of the bladder carcinogen 2-amino-4-(5-nitro-2-furyl)thiazole (anft) was investigated by comparison with benzidine. in comparison with benzidine, anft has a higher electrochemical potential (approximately 700 mv) and is less effective as a reducing co-substrate for either prostaglandin h synthase (phs) or horseradish peroxidase. activation was monitored by measuring binding to protein (bsa) and dna. anft binding to protein was reduced by indomethacin, a fatty acid cyc ... | 1990 | 2121382 |
| the role of hydrogen peroxide in the in vitro cytotoxicity of 3-hydroxykynurenine. | previous studies have indicated that the generation of h2o2 may be a key step in the mechanism mediating the in vitro cytotoxicity of 3-hydroxykynurenine (3hk). an exposure protocol resulting in a delayed toxicity was utilized in order to further examine the role of h2o2 in the in vitro toxicity of 3hk in a neural hybrid cell line. 3hk-induced cell lysis was significantly attenuated by administration of catalase after termination of 3hk exposure and was abolished when intracellular peroxidase ac ... | 1990 | 2089269 |
| [extraction of horseradish peroxidase and its conjugates formed by surface-active agent micelles]. | the author studied peculiarities of the extraction of horseradish peroxidase (hrp) and its conjugates with 3 and 7 molecules of progesterone (prog) from the aqueous solution into heptane and chloroform containing reversed micelles of surfactants. micelles of cetyltrimethylammonium bromide, aerosol ot, and triton x-45 protect the enzyme from denaturation in the biphasic system. the enzyme is readily extracted from the aqueous phase in the organic medium containing reversed micelles of surfactants ... | 2013 | 2082348 |
| further study of the outward displacement of retinal ganglion cells during optic nerve regeneration, with a note on the normal cells of dogiel in the adult frog. | in a previous study we observed massive retinal ganglion cell death in adult rana pipiens after periods of optic nerve regeneration, and reported that large numbers of the surviving cells had become displaced bodily into the inner plexiform layer of the affected eye (scalia et al.: brain research 344:267-280, 1985). the outwardly displaced cells could be identified as retinal ganglion cells because they could be back-filled with horseradish peroxidase (hrp) injected into the regenerated optic ne ... | 1990 | 2077052 |
| direct carbohydrate analysis of glycoproteins electroblotted onto polyvinylidene difluoride membrane from sodium dodecyl sulfate-polyacrylamide gel. | a procedure for the carbohydrate analysis of glycoproteins electrotransferred to a polyvinylidene difluoride membrane is described. the glycoproteins (plant lectins, transferrin, and vitronectin) were first separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and then electroblotted onto a membrane. each of the glycoprotein bands visualized by staining with coomassie brilliant blue r-250 was excised from the membrane and subjected to direct hydrolysis either in 2.5 m ... | 1990 | 1705391 |
| age-related changes in the subcortical afferents to the medial frontal cortex in mice: a wga-hrp study. | the subcortical afferent projections to the mediodorsal part of the frontal cortex were studied both qualitatively and quantitatively in young (3 months), adult (12 months) and aged (22 months) balb/c mice by means of the retrograde transport of wheat germ agglutinine-horseradish peroxidase (wga-hrp). a progressive decrease in the number of afferents was observed during aging with a differential pattern of reduction as a function of the subcortical structures. in adult mice a large reduction of ... | 1990 | 2097591 |
| cells of origin of ascending and descending as well as branching fibers in the cervical spinal cord of the pigeon. | ascending and descending projections of spinal neurons (cervical enlargement) were studied with the retrograde transport of horseradish peroxidase (hrp) and with the fluorescent tracers fast blue and rhodamine isothiocyanate (single and double labeling). ascending and descending projections arise from the same laminae of the spinal grey except for neurons in contralateral lamina i and avian cervical clarke's column which have ascending fibers only. a significant number of cervical nucleus propri ... | 1990 | 2097571 |
| interaction of aromatic donor molecules with manganese(iii) reconstituted horseradish peroxidase: proton nuclear magnetic resonance and optical difference spectroscopic studies. | the interaction of aromatic donor molecules with manganese(iii) protoporphyrin-apohorseradish peroxidase complex [mn(iii)hrp] was investigated by optical difference spectroscopy and relaxation rate measurements of 1h resonances of aromatic donor molecules (at 500 mhz). ph dependence of substrate proton resonance line-widths indicated that the binding was facilitated by protonation of an amino acid residue (with a pka of 6.1), which is presumably distal histidine. dissociation constants were eval ... | 1990 | 2223851 |
| the distribution and morphology of corticogeniculate axons in ferrets. | this study examined the distribution of axons in the lateral geniculate (lgn) and adjacent nuclei following injections of wheat germ agglutinin bound to horseradish peroxidase (wga-hrp), [3h]proline and biocytin into area 17 in ferrets. terminal label was found in the lgn, perigeniculate (pgn), medial interlaminar (min) and lateral posterior (lp) nuclei. lgn label was more concentrated in the interlaminar zones. analysis of axonal morphology showed that different axon types project to lgn, min a ... | 1990 | 2271942 |
| development of connections to and from the visual cortex in the wallaby (macropus eugenii). | the time course of the development of connections between the visual cortex and the main subcortical visual structures, as well as intrahemispheric and interhemispheric connections, has been studied in the marsupial wallaby (macropus eugenii) to compare its development with that of placental mammals. pouch young are born prior to retinal innervation of the primary visual centers and spend a protracted period of development in the pouch, making them ideal for visual, developmental studies. horser ... | 1990 | 2258462 |
| quantitative distribution of descending tectal efferent cells in salamanders. | in salamanders of the species salamandra salamandra, pleurodeles waltl, and plethodon jordani, tectal efferent neurons projecting to the bulbar reticular formation show a stereotyped distribution as revealed by the horseradish peroxidase (hrp) method. the density of these cells increases toward caudal tectal levels reaching its maximum at the isthmic region. neurons with ipsilaterally descending axons constitute 60-87% and are concentrated in the dorsomedial and ventrolateral sector of the tecta ... | 1990 | 2259459 |
| enhanced chemiluminescence determination of alpha-amylase by use of amylose labelled with horseradish peroxidase. | an enhanced chemiluminescence method for determining soluble and immobilized alpha-amylase has been developed, based on use of an insoluble amylose substrate labelled with horseradish peroxidase. soluble peroxidase-labelled fragments of the substrate, released by the action of alpha-amylase, are quantified by the peroxidase-catalyzed luminol-peroxide-p-iodophenol reaction. the detection limit for alpha-amylase was 125 fmole (12.5 nm). an insoluble amylopectin labelled with horseradish peroxidase ... | 1990 | 18965051 |
| construction of a fast, inexpensive rapid-scanning diode-array detector and spectrometer. | a 512-element diode-array spectroscopic detection system capable of acquiring multiple spectra at a rate of 5 ms per spectrum with an effective scan rate of 102.9 khz has been constructed. spectra with fewer diode elements can also be acquired at scan rates up to 128 khz. the detector utilizes a hamamatsu silicon photodiode-array sensor that is interfaced to hamamatsu driver/amplifier and clock generator boards and a dra laboratories 12-bit 160-khz analog-to-digital converter. these are standard ... | 1990 | 2285140 |
| neuronal structure of the lacertilian parietal eye, i: a retrograde label and electron-microscopic study of the ganglion cells in the photoreceptor layer. | the cellular connectivity of the lacertilian parietal eye is not well understood. because the intercellular connections establish the foundation for information processing, we have investigated cellular connectivity of one cell type in this simple vertebrate retina. we also developed an in vitro preparation to study the anatomy of the parietal eye visual system. horseradish peroxidase transport in the in vitro preparation revealed a class of displaced ganglion cells occupying positions among the ... | 1990 | 2265152 |
| neuronal adaptation accompanying metamorphosis in the flatfish. | flatfish provide a natural paradigm to investigate adaptive changes in the central nervous system of vertebrates. during their metamorphosis, the animals undergo a 90 degrees tilt to one side or the other to become the bottom-adapted adult flatfish. the eye on the down side is pushed over to the up side. thus, vestibular and oculomotor coordinate systems rotate 90 degrees relative to each other. as a result, during swimming movements different types of compensatory eye movements are produced bef ... | 1990 | 2258726 |
| biochemical and immunological studies of proteins from polydnavirus chelonus sp. near curvimaculatus. | polydnavirus from chelonus sp. curvimaculatus (ccv) was purified and 18 structural polypeptides associated with ccv were identified by silver staining. antibodies were raised against ccv protein and used in testing for ovary and in stung eggs. it was also established that the virus does not enter during the first 5 s of oviposition. furthermore, no degradation of the virus proteins was detected inside the egg within 2 h after oviposition. the glycoprotein nature of virus proteins was also determ ... | 1990 | 2230739 |
| transcellular processing of disulfide- and thioether-linked peroxidase--polylysine conjugates in cultured mdck epithelial cells. | horseradish peroxidase (hrp) was conjugated to nondegradable polycationic poly(d-lysine) (pdl) through either a thioether (hrp-s-pdl) or a disulfide (hrp-ss-pdl) linkage. the binding and transcytosis of these conjugates was studied in madin-darby canine kidney (mdck) cell monolayers grown on 3-microns microporous polycarbonate filters. conjugation of hrp to pdl with both linkages markedly increased the binding of this protein onto the cell monolayers. however, an enhancement of the transcellular ... | 1990 | 2211845 |
| [study of the glycoconjugates in the mesonephros of the chick embryo using peroxidase-conjugated lectin]. | the distribution of the sugar residues in glycoconjugates along the mesonephric nephron of chick embryo from the 4th day of incubation till hatching has been investigated, by means of six different horseradish peroxidase-labelled lectins. cona and wga showed an ubiquitous presence of alpha-d-mannose and n-acetyl-d-glucosamine along the nephrons. sba was found to be a specific marker of the proximal tubule. pna and lta reacted only for a short time at some sites during the considered period of in ... | 1990 | 2096878 |
| immunochemical evidence for intramolecular interaction of the carboxy terminal a alpha-appendages of plasma fibrinogen. | a monoclonal antibody (mab), 45j, which reacts with intact fibrinogen, has been employed to demonstrate the interaction of the carboxy terminal regions of the a alpha-chain in non-denatured plasma fibrinogen. the 45j mab recognizes an epitope in the mid section of the carboxy terminal end of the a alpha chain. the epitope is destroyed by plasmin and trypsin digestion. the 45j mab and a horseradish peroxidase conjugate of the 45j mab (45j-hrp) were used in an elisa to demonstrate that the antibod ... | 1990 | 1723303 |
| the application of immunocytochemical techniques to routinely-fixed and stained cytologic specimens. an aid in the differential diagnosis of undifferentiated malignant neoplasms. | routinely-fixed and papanicolaou stained smears with the cytologic diagnosis of undifferentiated malignant neoplasm that had been prepared with cells obtained by fine needle aspiration biopsy (n = 7), pulmonary lavage (n = 5), or thoracentesis (n = 3) from 15 unselected patients were stained by an immunocytochemical technique to evaluate the presence of keratin proteins and the leukocyte common antigen (lca). commercially available, well-characterized monoclonal antibodies with specificities for ... | 1990 | 1705023 |
| novel and sensitive noncompetitive (two-site) immunoassay for haptens with emphasis on peptides. | a novel and sensitive noncompetitive (two-site) enzyme immunoassay is described to measure attomole amounts of haptens, especially small peptides, which cannot be bound simultaneously by two different antibodies directed to the corresponding two different epitopes on the hapten molecules; consequently, they cannot be measured by the conventional two-site immunoassays. the principle of the assay is to label haptens to be measured with an appropriate substance, so that the hapten molecule can be b ... | 1990 | 1701368 |
| differential organization of crossed and uncrossed projections from the superior olive to the inferior colliculus in the mole. | projections from the medial (mso) and lateral (lso) superior olivary nuclei to the inferior colliculus (ic) were examined in the mole. in each mole, fluoro-gold was injected into one ic and wheat germ-agglutinated horseradish peroxidase (wga-hrp) into the other ic; most mso neurons were double-labeled bilaterally, while most lso neurons were single-labeled bilaterally. the results indicate that the bilateral projections from the mso and lso to the ic are mainly established by single mso neurons ... | 1990 | 1705316 |
| luminol chemiluminescence reaction catalyzed by a microbial peroxidase. | a peroxidase produced by microorganisms belonging to the genera arthromyces and coprinus was found to be a potent catalyst for the chemiluminescent oxidation of luminol, the luminescence produced per unit of microbial peroxidase protein being well over 100 times as strong as that produced by horseradish peroxidase. no large difference in km value for h2o2 in the presence of luminol was found between arthromyces ramosus peroxidase and horseradish peroxidase (7.0 and 15.5 microm, respectively), bu ... | 1990 | 2281860 |
| fate mapping the avian epiblast with focal injections of a fluorescent-histochemical marker: ectodermal derivatives. | a microinjection technique is described for fate mapping the epiblast of avian embryos. it consists of injecting the epiblast of cultured blastoderms with a fluorescent-histochemical marker, examining rhodamine fluorescence at the time of injection in living blastoderms, and assaying for horseradish peroxidase activity in histological sections obtained from the same embryos collected 24 h postinjection. our results demonstrate that this procedure routinely marks cells, allowing their fates to be ... | 1990 | 2203877 |
| origin of infrasound sensitive neurones in the papilla basilaris of the pigeon: an hrp study. | infrasound sensitive afferent fibres recorded in the pigeon cochlear ganglion were marked by intracellular injections of horseradish peroxidase (hrp). all stained fibres were found to innervate hair cells in the basilar membrane between 90 and 950 microns from its apical end. no fibres to the macula lagenae were found. nine of the 10 completely stained fibres contacted hair cells located abneurally on the free basilar membrane, the tenth ended over the neural limbus near its abneural border. all ... | 1990 | 1701169 |
| cytoarchitecture and fiber connections of the nucleus lateralis valvulae in the carp (cyprinus carpio). | the cytoarchitecture and fiber connections of the nucleus lateralis valvulae of the carp (cyprinus carpio) were studied by nissl, bodian, golgi, and horseradish peroxidase methods. chief cells composing the nucleus lateralis valvulae (nlv) are small and granular, and their axons terminate in the cerebellum. these neurons have no dendrite, and the cell body is enveloped in a single terminal of afferent fibers to the nlv. in order to observed local cell clustering, the nlv was three-dimensionally ... | 1990 | 2229471 |
| a different type of vertebrate thalamic organization. | the presence of a reptilian thalamic reticular nucleus was investigated in caiman crocodilus. injections of horseradish peroxidase made into the dorsal thalamus produced retrogradely labeled neurons in the nucleus of the dorsal peduncle of the lateral forebrain bundle. a separate population of thalamic neurons located in the nucleus of the dorsal peduncle of the lateral forebrain bundle with morphology different from those neurons projecting to the dorsal thalamus were found to be immunoreactive ... | 1990 | 1701334 |
| peripheral and central terminations of hypoglossal afferents innervating lingual tactile mechanoreceptor complexes in fringillidae. | injections of cholera toxin b subunit conjugated to horseradish peroxidase (ctb-hrp) were made into the lingual branch of the hypoglossal nerve in four species of finch in order to identify the innervation of the mechanoreceptors of the dermal papillae of the tongue, and simultaneously to determine the pattern of central projections of lingual hypoglossal afferents. the results showed that hypoglossal fibers innervate all the herbst corpuscles and terminal cell receptors of the elaborately organ ... | 1990 | 1698831 |
| [serorrhaphy prevents regeneration of vagus nerve fibers after proximal gastric vagotomy]. | spouting or regeneration of vagus fibers occurs after vagotomy. an axonal tracing method was used to see the long-term effect of serorrhaphy in the prevention of axonal regeneration after proximal gastric vagotomy (pgv) in ferrets. | 1990 | 2233669 |
| one-electron oxidation of trolox c (a vitamin e analogue) by peroxidases. | the oxidation mechanism of trolox c (a vitamin e analogue) by peroxidases was examined by stopped flow and esr techniques. the results revealed that during the oxidation of trolox c, peroxidase compound ii was the catalytic intermediate. the rate constants for the reaction of compound ii with trolox c, which should be the rate-determining step, were estimated to be 2.1 x 10(4) and 7.2 x 10(3) m-1.s-1 for horseradish peroxidase and lactoperoxidase, respectively, at ph 6.0. the formation of the tr ... | 1990 | 2172226 |
| analysis of neuronal networks: a review of techniques for labeling axonal projections. | in order to analyze connections between neurons in the vertebrate central nervous system, methods have been developed to label a given population of axons of known origin so that they can be differentiated from other, non-labeled structures. three such methods are reviewed here: experimentally induced orthograde (wallerian) degeneration, axon transport of radioactive proteins demonstrated by autoradiography, and axon transport of macromolecules that can be reacted histochemically to yield a visi ... | 1990 | 1697336 |
| visual and electrosensory circuits of the diencephalon in mormyrids: an evolutionary perspective. | mormyrids are one of two groups of teleost fishes known to have evolved electroreception, and the concomitant neuroanatomical changes have confounded the interpretation of many of their brain areas in a comparative context, e.g., the diencephalon, where different sensory systems are processed and relayed. recently, cerebellar and retinal connections of the diencephalon in mormyrids were reported. the present study reports on the telencephalic and tectal connections, specifically in gnathonemus p ... | 1990 | 2384612 |
| lignin peroxidase compounds ii and iii. spectral and kinetic characterization of reactions with peroxides. | stopped-flow techniques were used to investigate the kinetics of the reaction of lignin peroxidase compounds ii and iii (lipii and lipiii) with peroxides. rate data were obtained from single-turnover experiments under pseudo-first-order conditions. lipii reacts with h2o2 or peracetic acid (acooh) to form a modified lipiii, designated as lipiii*, via a biphasic reaction. during the first phase, lipiii is formed as an intermediate. kinetic analysis also indicates a lipii-peroxide complex. the firs ... | 1990 | 2162833 |
| antigenic relationships between petunia peroxidase a and specific peroxidase isoenzymes in other solanaceae. | a highly specific rabbit antiserum raised against peroxidase (prxa) from petunia (petunia hybrida) was used to investigate the antigenic relatedness of peroxidases in the solanaceae. after sds-page of crude leaf extracts from a large number of species of this family, immunoblotting revealed that cross-reacting protein bands were present in all species tested. in order to determine whether these protein bands represent peroxidases, the peroxidase isoenzymes in thorn apple (datura stramonium l.), ... | 1990 | 24220819 |
| effect of indomethacin on binding of bilirubin to albumin. | the effect of indomethacin on the binding of bilirubin to protein was studied in vitro by the horseradish peroxidase oxidation method. plasma indomethacin concentrations ranging from 1500 micrograms/l to 4500 micrograms/l, which were much higher than the therapeutic concentrations achieved in vivo, were used for the studies. the bilirubin-protein titration curves obtained indicate that indomethacin, although protein bound, does not affect the binding of bilirubin to protein. our study confirms t ... | 1990 | 2386402 |
| inactivation of lignin peroxidase by phenylhydrazine and sodium azide. | lignin peroxidase (lip) is rapidly inactivated in a concentration-dependent manner by h2o2 and either phenylhydrazine or sodium azide. full inactivation of isozyme 2b (h8) requires approximately 50 eq of phenylhydrazine or 80 eq of sodium azide. anaerobic incubation of isozyme 2b with [14c]phenylhydrazine and h2o2 results in 77% loss of catalytic activity and covalent binding of 0.45 mol radiolabel/mol of enzyme. comparable but not identical results are obtained with an isozyme mixture. a lag pe ... | 1990 | 2353822 |
| multitubular bodies in intestinal cells of amphiuma means/tridactylum (urodela): ultrastructural characterization. | the jejunal absorptive cells of the salamander amphiuma, when examined using transmission electron microscopy, were found to possess a unique type of intracellular vacuole containing membranous tubules. these vacuoles, tentatively named multitubular bodies, were located in the cytoplasm between the nucleus and the brush-border membrane, and were seen with greatest frequency in the summer and fall. the vacuoles containing multitubular bodies had an average diameter of 0.6 microns, and the membran ... | 1990 | 2166623 |
| influence of the reaction medium on the product distribution of peroxidase-catalysed oxidation of p-cresol. | p-cresol was oxidized by hydrogen peroxide in a reaction catalysed by horseradish peroxidase and the low molecular weight products were investigated. in aqueous media pummerer's ketone (i) was the dominating product but in organic media the product distribution was quite different; 2,2'-dihydroxy-5,5'-dimethyldiphenyl (ii) was the main low molecular weight product. similar product distributions were obtained with peroxidase adsorbed on a solid support and suspended in toluene and with peroxidase ... | 1990 | 1366748 |
| spatial organization of axons in turtle visual cortex: intralamellar and interlamellar projections. | the spatial pattern of projections within turtle visual cortex was studied by using focal injections of horseradish peroxidase into visual cortex in an in vitro wholebrain preparation. injections anterogradely filled the axons of many layer 2 neurons, which could be followed for 200-500 microns from the injection sites. axons were typically unbranched, relatively straight, and bore small varicosities at irregular intervals. they radiated from the injection sites in all directions, but showed som ... | 1990 | 2358552 |
| organization of geniculocortical projections in turtles: isoazimuth lamellae in the visual cortex. | the projection from the dorsal lateral geniculate complex to the visual cortex in pseudemys and chrysemys turtles was examined by using the anterograde transport of horseradish peroxidase (hrp) in vitro and the retrograde transport of hrp in vivo. in vitro hrp injections into the lateral forebrain bundle were used to fill geniculocortical axons anterogradely, which were then analyzed in cortical wholemount preparations. geniculocortical axons gain access to the visual cortex along its entire ros ... | 1990 | 2358551 |
| ultrastructure of an identified molluscan neuron in organ culture and cell culture following axotomy. | we examined the ultrastructure of neuron 5 from the buccal ganglion of the mollusc helisoma trivolvis after axotomy and organ culture, and after isolation of the same neuron in culture. buccal ganglia containing axotomized neurons 5 were cultured either in host snails or in leibovitz medium conditioned with ganglia. in addition, some neurons 5 were isolated from buccal ganglia by micro-dissection and plated into culture. neuron 5 and its processes were identified in both whole mounts and plastic ... | 1990 | 2358546 |
| anions activate the oxidation of indoleacetic acid by peroxidases from tomato and other sources. | anionic peroxidase from tomato (lycopersicon esculentum) fruit oxidized indoleacetic acid (iaa) slowly in the presence of mn(2+) and dichlorophenol in acetate buffers. the addition of certain anions to the reaction mixture increased the rate of oxidation. phosphate was one of the effective anions and exerted maximal activation at 0.1 molar. the most effective activator of tomato peroxidase was nitrilotriacetate (nta) at an optimum concentration of 60 micromolar. only 0.17 nanomolar peroxidase wa ... | 1990 | 16667539 |
| the peroxidase-promoted metabolic activation of acetaminophen produces electronically excited species. | when the analgesic and antipyretic drug acetaminophen is exposed to the horseradish peroxidase/hydrogen peroxide system, light emission is observed. both the rate of reaction and the emission intensity are increased in the presence of the cationic detergent ctab. the emission spectrum indicates the presence of at least three emitters. the peak at 445 nm is close to the position of the only band observed in the fluorescence spectrum of the spent reaction mixture and is ascribed to excited n-acety ... | 1990 | 2367566 |
| dendritic distribution of two populations of ganglion cells and the retinopetal fibers in the retina of the silver lamprey (ichthyomyzon unicuspis). | the distribution of ganglion cells in the retina of the silver lamprey, ichthyomyzon unicuspis, was revealed by retrograde labeling from the optic nerve with horseradish peroxidase (hrp) and fluorescent-labeled dextrans in live animals and with the fluorescent dye dii in aldehyde-fixed tissue. the majority of ganglion cells (74%) termed the "outer ganglion cells," are multipolar and are located at the vitread boundary of the inner nuclear layer. the remaining ganglion cells (26%), termed the "in ... | 1990 | 2278933 |
| a light- and electron-microscopic investigation of the optic tectum of the frog, rana pipiens, ii: the neurons that give rise to the crossed tecto-bulbar pathway. | the superficial layers of the frog's optic tectum, potter's (1969) layers a-g, comprise a complex neuropil made up of many afferent axons, the somata of a few neurons, and many dendrites from the neurons located in the deeper layers. different types of retinal axons are believed to terminate in different layers (maturana et al., 1960; kuljis & karten, 1988; sargent et al., 1989), but little is known about the relationships between each type of input and the dendrites of the deep tectal neurons t ... | 1990 | 2278932 |
| a light- and electron-microscopic investigation of the optic tectum of the frog, rana pipiens, i: the retinal axons. | there are several different groups of ganglion cells in the retina of the frog. although their axons are thought to terminate in different layers of the optic tectum, little is known about the morphology of their terminal arbors or their synaptic targets. the present paper reports the results of a layer-by-layer study of horseradish peroxidase labeled retinal axons in the optic tectum of rana pipiens. light and electron microscopy was used to study the axon's laminar distribution, patterns of ar ... | 1990 | 2278931 |
| a 'puff and advance' technique for visually controlled staining of turtle retinal ganglion cells. | we describe a 'puff and advance' technique for visually controlled staining of retinal ganglion cells (gcs) in the unfixed, living retina for light and electron microscopy. glass microelectrodes are filled with rhodamine-isothiocyanate labeled horseradish peroxidase (rh-hrp), or lucifer yellow (ly), or a mixture of both, or with 5,6-carboxytetramethylrhodamine (5,6-rh) and advanced tangentially through the gc layer with microscopic observation using epifluorescence. brief "puffs" of ly or 5,6-rh ... | 1990 | 1696675 |
| differences in lectin binding patterns of normal endometrium and endometrial adenocarcinoma, with special reference to staining with ulex europeus agglutinin 1 and peanut agglutinin. | differences in glycoconjugate composition between proliferative endometrium and endometrial adenocarcinoma were investigated by histochemical techniques using seven different lectins as probes. for light microscopy, the avidin-biotin-peroxidase complex (abc) method was used. concanavalin a (con a), wheat germ agglutinin (wga), and ricinus communis agglutinin (rca) stained almost all glandular cells in both proliferative (normal) and malignant endometria. ulex europeus agglutinin 1 (uea-1) strong ... | 1990 | 1693583 |
| mauthner cells maintain their lumbar projection in adult frog. | we have used retrograde labeling with horseradish peroxidase (hrp) in the bullfrog. rana catesbeiana, to determine whether mauthner (m) cells maintain a projection to the lumbar spinal cord in adult bullfrogs. we found that m cells persist in the adult bullfrog and maintain a projection to the lumbar spinal cord, despite the degeneration of much of their afferent input and of their motoneuronal targets in the spinal cord. | 1990 | 2377313 |
| central projections of cochlear nerve fibers in the alligator lizard. | the auditory (cochlear) ganglion cells of the alligator lizard (gerrhonotus multicarinatus) give rise to two types of peripheral fibers: tectorial fibers, which contact hair cells covered by a tectorial membrane, and free-standing fibers, which contact hair cells without a tectorial membrane. to determine the central projections of these fibers, we applied intracellular and extracellular injections of horseradish peroxidase (hrp) to the peripheral component of the cochlear nerve. after histologi ... | 1990 | 2358519 |
| fibre organization of the monkey's optic tract: i. segregation of functionally distinct optic axons. | the fibre organization of the monkey's optic tract was examined by implanting pellets of horseradish peroxidase into different locations within the tract, or into the superior colliculus and pretectum. retinae were examined for the distribution, size, and morphological types of retrogradely labelled ganglion cells; optic tracts were examined for the distribution of anterogradely and retrogradely labelled axonal profiles; and lateral geniculate nuclei were examined for the distribution of anterog ... | 1990 | 2351758 |
| genomic dna structure of two new horseradish-peroxidase-encoding genes. | genomic dnas encoding the horseradish peroxidase (hrp) isozymes, prxc2 and prxc3, were cloned and sequenced. by comparing the sequences of the hrp isozyme-encoding genes, prxc1a and prxc1b and their cdna [fujiyama et al., eur. j. biochem. 173 (1988) 681-687], , it was concluded that prxc2 and prxc3 consisted of four exons and three introns as in the prxc1 gene family. the position of introns in coding regions were the same in all four prx genes. genes prxc2 and prxc3 coded for 347 and 349 amino ... | 1990 | 2373366 |
| organization of the primary projections of the lateral line nerves in the lamprey lampetra japonica. | the lateral line sensory system of lampetra japonica is innervated by the anterior and posterior lateral line nerves. the anterior lateral line nerve innervates all electroreceptors throughout the body and mechanoreceptors of the head. the posterior lateral line nerve innervates trunk mechanoreceptors. the anterior lateral line nerve consists of two ganglia (anterior lateral line and intracapsular) and four major peripheral branches (superficial ophthalmic, buccal, hyomandibular, and recurrent n ... | 1990 | 2358517 |
| coordination of reinnervated muscle and reorganization of spinal cord motoneurons after nerve transection in mice. | combining physiological and anatomical methods, the dependence of muscle coordination on the alteration in connections between anterior horn motoneurons and muscle following peripheral nerve repair has been examined in adult mice. fourteen weeks after transection and readaptation of the right common peroneal nerve electromyographic activity patterns of the tibialis anterior (ta) and medial gastrocnemius (mg) muscles were recorded simultaneously during free running. the number and location of mot ... | 1990 | 2357563 |
| analysis of hydrogen peroxide in freshly prepared coffees. | coffee has been shown unequivocally to be genotoxic in vitro, but no genotoxicity has been seen in vivo testing. since the in vitro genotoxicity appears to be dependent on hydrogen peroxide, it is important to know whether hydrogen peroxide is present in prepared coffee and whether it is being formed during the in vitro testing. we have devised a procedure to measure hydrogen peroxide in prepared coffee using disposable reversed-phase columns to decolourize the coffee and retain its catechols. h ... | 1990 | 2379891 |
| nerve growth factor in skeletal tissues of the embryonic chick. | this study demonstrates, via immunohistochemistry and bioassay, the presence of ngf in embryonic bone and cartilage of the chick. embryos were killed on days 6-9 of incubation at 12 h intervals, and on days 10-18 at 24 h intervals. paraffin-embedded sections of hind limbs or buds were immunostained with a polyclonal antibody against ngf and the biotin-avidin-horseradish peroxidase technique. immunostaining was positive in both bone and cartilage, with cartilage staining more intensely. for bioas ... | 1990 | 2372809 |
| kinetic and molecular orbital studies on the rate of oxidation of monosubstituted phenols and anilines by horseradish peroxidase compound ii. | the second-order rate constant (k4) for the oxidation of a series of aromatic donor molecules (monosubstituted phenols and anilines) by horseradish peroxidase (hrp) compound ii was examined with a stopped-flow apparatus. the electronic states of these substrates were calculated by an ab initio molecular orbital method. it was found that in both phenols and anilines log k4 values correlate well with the highest occupied molecular orbital (homo) energy level and the lowest unoccupied molecular orb ... | 1990 | 2361133 |
| morphology and physiology of cells in slice preparations of the posteroventral cochlear nucleus of mice. | in an effort to understand what integrative tasks are performed in the cochlear nuclei, the present study was undertaken to describe neuronal circuits in the posteroventral cochlear nucleus (pvcn) anatomically and physiologically. the cochlear nuclear complex receives auditory information from the cochlea through the auditory nerve. within the cochlear nuclei, signals travel along several parallel and interconnected pathways. from the cochlear nuclei, transformed versions of the signals are pass ... | 1990 | 2341631 |
| neural elements in the pineal complex of the frog, rana esculenta, i: centrally projecting neurons. | the pineal complex of anuran amphibians is a directly photosensory organ, encompassing both an extracranial portion, the frontal organ, and an intracranial portion, the pineal organ proper. the projection neurons of the frontal organ respond differentially according to the wavelengths of the light stimuli. the pineal organ, on the other hand, functions mainly as a luminosity meter. most of its centrally projecting neurons respond to all increases in ambient illumination with decreases in spontan ... | 1990 | 2271451 |
| the application of enhanced chemiluminescence to membrane-based nucleic acid detection. | the ecl gene detection system is a novel, sensitive, non-radioactive system for the detection of nucleic acid hybridized on both nylon and nitrocellulose membranes. it is characterized by direct labeling of probe sequences with horseradish peroxidase combined with an enhanced chemiluminescent (ecl) detection reaction; the light output is captured on blue-light sensitive film. the application of the system to a range of standard molecular biology hybridization techniques is described. | 1990 | 2192738 |
| neural elements in the pineal complex of the frog, rana esculenta, ii: gaba-immunoreactive neurons and fmrfamide-immunoreactive efferent axons. | the photosensory pineal complex of anurans comprises an extracranial part, the frontal organ, and an intracranial part, the pineal organ proper. although the pineal organ functions mainly as a luminosity detector, the frontal organ may monitor the relative proportions of short and intermediate/long wavelengths in the ambient illumination. the major pathway of information processing in the pineal and frontal organs is the photoreceptor to ganglion cell synapse. it is not known whether interneuron ... | 1990 | 2176814 |
| parathyroid hormone action on phosphate transport is inhibited by high osmolality. | parathyroid hormone (pth) produces rapid inhibition of na(+)-phosphate cotransport, characterized by a decreased maximal rate of transport, and the inhibition is independent of de novo protein synthesis. the present study determined whether the action of pth on na(+)-phosphate cotransport is mediated, at least in part, by rapid endocytic internalization of na(+)-phosphate cotransporters present in the plasma membrane. horseradish peroxidase, a fluid-phase marker, was used to demonstrate the pres ... | 1990 | 2159721 |
| immunocytochemical characterization of supporting cells in the enteric nervous system in hirschsprung's disease. | the enteric nervous system (ens) is composed of two distinct neural components, extrinsic and intrinsic, and its supporting cells uniquely possess some characteristics of both central nervous system (cns) astrocytes and peripheral nervous system (pns) schwann cells. to provide further insight into the neural defects in hirschsprung's disease, the supporting cells in biopsied normal gut, ganglionic, and aganglionic segments from six cases of hirschsprung's disease were investigated immunocytochem ... | 1990 | 1972188 |
| lympho-epithelial interactions in the turtle chrysemys scrypta elegans. | the morphological relationships present in the areas of lymphoid infiltration found in the esophagus in chrysemys scripta elegans were studied by the use of light and transmission electron microscopy. these infiltrations are widespread and can be observed in the lamina propria and the covering epithelium. electron microscopy reveals the presence of epithelial cells with differing characteristics at the apex of the lymphoid infiltrations. the introduction of horseradish peroxidase (hrp) into the ... | 1990 | 1695064 |
| two-dimensional 1h-nmr studies of horseradish peroxidase c and its interaction with indole-3-propionic acid. | the binding of aromatic donor molecules to plant peroxidases has been investigated by examining the complex formed between horseradish peroxidase isoenzyme c and indole-3-propionic acid using two-dimensional 1h-nmr spectroscopy. despite the relatively high molecular mass and paramagnetism of the protein, this technique can be successfully applied to provide new information on the structure of the complex. a number of relatively well-resolved resonances in certain regions of the one-dimensional s ... | 1990 | 2338080 |
| binding of thiocyanate and cyanide to manganese(iii)-reconstituted horseradish peroxidase: a 15n nuclear magnetic resonance study. | binding of thiocyanate and cyanide ions to mn(iii) protoporphyrin-apohorseradish peroxidase complex [mn(iii)hrp] was investigated by relaxation rate measurements (at 50.68 mhz) of 15n resonance of sc15n- and c15n-. at ph = 4.0 the apparent dissociation constant (kd) for thiocyanate and cyanide binding to mn(iii)hrp was deduced to be 156 and 42 mm, respectively. the ph dependence of the 15n line width as well as apparent dissociation constant for thiocyanate and cyanide binding were quantitativel ... | 1990 | 2158822 |
| oxygen diffusion through horseradish peroxidase. | the quenching by molecular oxygen of the fluorescence from a protoporphyrin ix adduct of horseradish peroxidase has been investigated using both intensity and time-resolved techniques. the bimolecular quenching rate constant determined for this process, as evaluated by the conventional stern-volmer analysis, was 2 x 10(8) m-1 s-1, among the lowest observed for protein systems. this result suggests that the heme binding site in horseradish peroxidase is relatively inaccessible to oxygen, which ma ... | 1990 | 2343064 |
| the vestibular nerve of the chinchilla. v. relation between afferent discharge properties and peripheral innervation patterns in the utricular macula. | 1. the relation between the discharge properties of utricular afferents and their peripheral innervation patterns was studied in the chinchilla by the use of intra-axonal labeling techniques. fifty-three physiologically characterized units were injected with horseradish peroxidase (hrp) or lucifer yellow ch (ly) and their labeled processes were traced to the utricular macula. for most labeled neurons, the discharge regularity, background discharge, and sensitivity to externally applied galvanic ... | 1990 | 2341877 |
| the vestibular nerve of the chinchilla. iii. peripheral innervation patterns in the utricular macula. | 1. nerve fibers supplying the utricular macula of the chinchilla were labeled by extracellular injection of horseradish peroxidase into the vestibular nerve. the peripheral terminations of individual fibers were reconstructed and related to the regions of the end organ they innervated and to the sizes of their parent axons. 2. the macula is divided into medial and lateral parts by the striola, a narrow zone that runs for almost the entire length of the sensory epithelium. the striola can be dist ... | 1990 | 2341875 |
| reliability of prenatal diagnosis of genetic diseases by analysis of amplified trophoblast dna. | dot blot analysis on enzymatically amplified trophoblast dna with allele specific oligonucleotide probes is currently used for the prenatal diagnosis of single gene disorders characterised at the molecular level, such as the beta thalassaemias, phenylketonuria, sickle cell anaemia, and alpha 1-anti-trypsin deficiency. a potential problem with the use of this procedure is the co-amplification of maternal sequences, which may obscure the diagnosis in the fetus. to address this question, we carried ... | 1990 | 2325104 |
| reversible inhibition of endocytosis in cultured neurons from the drosophila temperature-sensitive mutant shibirets1. | the drosophila mutant, shibirets1 (shits1), is paralyzed at restrictive temperatures (greater than 29 degrees c) by a reversible block in synaptic transmission. heat pulses deplete synaptic vesicles in nerve terminals and inhibit endocytic internalization of plasma membrane in garland cells and oocytes. in dissociated cultures of larval central nervous system (cns), a temperature-sensitive defect is also expressed in shits1 neurons: at 30 degrees c, growth cone formation is retarded and neurite ... | 1990 | 2113575 |
| endocytosis of wheat germ agglutinin binding sites from the cell surface into a tubular endosomal network. | by using fluorescence and electron microscopy, the endocytic pathway encountered by cell surface components after they had bound wheat germ agglutinin (wga) was visualized. the majority of these components are thought to consist of sialylated glycoproteins (hmwag) that represent a subpopulation of the total cell surface proteins but most of the externally disposed plasma membrane proteins of the cell. examination of semi-thin sections by medium- and high-voltage electron microscopy revealed the ... | 1990 | 1969417 |
| an enzyme immunoassay for galactosyltransferase isoenzyme ii, and its clinical application to cancer diagnosis. | in this "sandwich"-type enzyme immunoassay of galactosyltransferase isoenzyme ii (gt-ii), we used the monoclonal antibody mab 3872 previously established and characterized to be specific for gt-ii. plastic beads coated with mab 3872 were incubated with serum and buffer, washed, then incubated with mab 3872 conjugated with horseradish peroxidase and again washed. peroxidase activity remaining on the beads was measured by color development with o-phenylenediamine. the assay standard curve was line ... | 1990 | 1691054 |