| chemiluminescent and bioluminescent techniques. | light-emitting chemical reactions (chemiluminescence, cl) and biological reactions (bioluminescence, bl) have a diverse range of analytical applications but relatively few have been adopted by routine clinical laboratories. advantages of cl and bl assays include sensitivity (attomole and sub-attomole detection limits), speed (signal generated in a few seconds and in some cases stable for several hours), nonhazardous reagents, and simple procedures. the most promising clinical applications are in ... | 1991 | 1893571 |
| enzyme labeling in steroid enzyme immunoassays. comparison of the p-nitrophenyl ester and n-succinimidyl ester methods. | enzyme labeling of steroids by the p-nitrophenyl ester method was investigated in comparison with the n-succinimidyl ester method. the active ester of a testosterone or 11-deoxycortisol derivative was treated with beta-galactosidase and horseradish peroxidase to give labeled antigens. various molar ratios of steroid to enzyme and ph conditions were tested. satisfactory immunoreactivities with an anti-steroid antibody in each enzyme immunoassay system were obtained with the labeled antigens prepa ... | 1991 | 1804551 |
| kinetics of a rapid, luminol dependent chemiluminescence signal induced in hl-60 cells by amphotericin b and other stimulants. | addition of the polyene antibiotic amphotericin b or tissue culture medium to nondifferentiated hl-60 cells in the presence of luminol induces a chemiluminescence signal that reaches a peak value within a few seconds and decays exponentially in less than a minute. the kinetics of the signal and its modulation by superoxide dismutase, catalase, and horseradish peroxidase are consistent with a series of solution biochemical processes with a rate-determining step corresponding to the disproportiona ... | 1991 | 1793562 |
| postnatal maturation of spiral ganglion neurons: a horseradish peroxidase study. | using an in vitro cochlear preparation from postnatal hamsters, spiral ganglion cells (sgcs) were labeled retrogradely following extracellular injections of hrp into the cochlear nerve. in 24 cochleae from hamsters between postnatal days (p) 0 and 10, the neuronal morphology of 201 sgcs and their peripheral axons were analyzed. from p 0 to 3, labeled sgcs had few distinguishable features. although sgcs could be traced separately to inner hair cells (ihcs) and outer hair cells (ohcs), they all ha ... | 1991 | 1752797 |
| [immunoenzyme determination of the total level of ceruloplasmin in the serum from patients with hepatocerebral dystrophy]. | an immunoenzymatic method for ceruloplasmin analysis (iea) based on the use of horseradish peroxidase-labelled monospecific antibodies as markers has been developed. iea can be used for direct measurements of ceruloplasmin in blood serum, as can be evidenced from the coincidence of calibration plots obtained after the use of potassium-phosphate buffer and ceruloplasmin-free sera. the procedure allows the determination of the total content of ceruloplasmin present in the blood sera of patients wi ... | 1991 | 1747426 |
| cytoarchitecture and intrafrontal connections of the frontal cortex of the brain of the hamadryas baboon (papio hamadryas). | a study was made of the cytoarchitecture of the lateral and medial frontal cortex in the hamadryas baboon (papio hamadryas). the frontal cortico-cortical connections of areas 46, 8, 6, and 4 were investigated by injection of wheat-germ agglutinine conjugated to horseradish peroxiase (wga-hrp) into different regions of areas 46, 8, and 6. the lateral region of the frontal lobe of the baboon consists of broad areas of motor (area 4), premotor (area 6), and the dorsolateral prefrontal cortex, each ... | 1991 | 1719042 |
| effect of linking allyl and aromatic chains to histidine 170 in horseradish peroxidase. | histidine residues in horseradish peroxidase (hrp) were modified chemically with diethyl pyrocarbonate, 4,omega-dibromoacetophenone or diallylpyrocarbonate. histidines were chosen as his-170, the fifth ligand of the heme iron atom, forms part of the active site of this enzyme. good yields of hemoprotein were obtained in all cases. analysis by hplc of peptides obtained after tryptic digestion showed that his-170 of hrp was in fact modified. the specific activity remained satisfactory after chemic ... | 1991 | 1911843 |
| the peroxidase-catalyzed oxidation of enkephalins. | in vitro experiments are reported showing that leu-enkephalin and metenkephalin, in the presence of hydrogen peroxide, can be oxidized by horseradish peroxidase. the products formed are strongly fluorescent and characterized by absorption peaks with maxima at 290 nm and 315 nm. the effects of substrate and enzyme concentrations on the oxidation rate of enkephalins are described. amino acid analysis of the hydrolysates from peroxidase-treated enkephalins provides evidence for the presence of dity ... | 1991 | 1652945 |
| intramedullary connections of the rostral nucleus of the solitary tract in the hamster. | the rostral nucleus of the solitary tract (nst) figures prominently in the gustatory system, giving rise to ascending taste pathways that are well documented. less is known of the local connections of the rostral nst with sites in the medulla. this study defines the intramedullary connections of the rostral nst in the hamster. small iontophoretic injections of horseradish peroxidase (hrp), confined to the rostral nst, resulted in golgi-like filling of axons that exited the nst or that interconne ... | 1991 | 1747757 |
| morphological differentiation of distinct neuronal classes in embryonic turtle cerebral cortex. | as a starting point for understanding the development of the cerebral cortex in reptiles and for determining how reptilian cortical development compares to that in other vertebrate classes, we studied the appearance and morphological differentiation of cerebral cortical neurons in embryonic turtles. 3h-thymidine birthdate labeling and focal injections of horseradish peroxidase (hrp) in in vitro cortical slices revealed that replicating cells occupy the outer ventricular zone, and subsequently mi ... | 1991 | 1719040 |
| ipsilateral cortical connections of granular frontal cortex in the strepsirhine primate galago, with comparative comments on anthropoid primates. | modern studies of granular frontal cortex (gfc) in large-brained, anthropoid primates, such as macaca, indicate that this region is comprised of many areal subdivisions. these areas vary in their architectonic appearance and each has a distinctive, diverse set of corticocortical connections. the great extent of the gfc region in anthropoids, and its high degree of areal parcellation, suggest that some gfc areas may be specializations of anthropoids, not found in other mammals. to investigate thi ... | 1991 | 1719039 |
| proton nmr investigation into the basis for the relatively high redox potential of lignin peroxidase. | lignin peroxidase shares several structural features with the well-studied horseradish peroxidase and cytochrome c peroxidase but carries a higher redox potential. here the heme domain of lignin peroxidase and the lignin peroxidase cyanide adduct was examined by 1hnmr spectroscopy, including nuclear overhauser effect and two-dimensional measurements, and the findings were compared with those for horseradish peroxidase and cytochrome c peroxidase. structural information was obtained on the orient ... | 1991 | 11607206 |
| morphology of a sensory neuron in drosophila is abnormal in memory mutants and changes during aging. | several mutations in drosophila impair learning and the camp cascade. we report here that the fine morphology of an identified mechanosensory neuron is abnormal in two of these mutants, dunce (dnc) and rutabaga (rut). the neuron innervating the antero-notopleural bristle was filled with horseradish peroxidase and studied at the light- and electron-microscopy level. in the mutants dnc and rut, this neuron has an abnormally large number of side branches and varicosities in a defined segment of the ... | 1991 | 1714597 |
| free radical formation in the oxidation of malondialdehyde and acetylacetone by peroxidase enzymes. | malondialdehyde, a product of lipid peroxidation, and acetylacetone undergo one-electron oxidation by peroxidase enzymes to form free radical metabolites, which were detected with esr using the spin-trapping technique. the structures of the radical adducts were assigned using isotope substitution. with both malondialdehyde and acetylacetone and the enzymes myeloperoxidase and chloroperoxidase, carbon-centered radicals were detected. with horseradish peroxidase, a carbon-centered radical was init ... | 1991 | 1654844 |
| thiol and mn(2+)-mediated oxidation of veratryl alcohol by horseradish peroxidase. | horseradish peroxidase has been shown to catalyze the oxidation of veratryl alcohol (3,4-dimethoxybenzyl alcohol) and benzyl alcohol to the respective aldehydes in the presence of reduced glutathione, mncl2, and an organic acid metal chelator such as lactate. the oxidation is most likely the result of hydrogen abstraction from the benzylic carbon of the substrate alcohol leading to eventual disproportionation to the aldehyde product. an aromatic cation radical intermediate, as would be formed du ... | 1991 | 1650355 |
| prospective fate of early blastomeres in hydractinia echinata (cnidaria, hydrozoa). | blastomeres of two-cell, four-cell, and eight-cell embryos of hydractinia echinata were injected with horseradish-peroxidase (hrp) or fluorescein isothiocyanate (fitc)-dextran. the fate of the descendants of the injected blastomeres was followed until the planula larva had developed. the results obtained after hrp or fitc-dextran injection were essentially the same. blastomeres are equivalent up to the four-cell stage, i.e. half-blastomeres produce half of the ectoderm of the planula larva and q ... | 1991 | 28306018 |
| [the number and soma size of retinal ganglion cells in chinese hamsters]. | the number and somatic size of retinal ganglion cells in chinese hamsters were examined by means of the horseradish peroxidase (hrp) staining method. the hrp staining is a method by which only the retinal ganglion cells can be stained. the number of ganglion cells per eye was 67,636-79,545 (mean: 73,575, n = 3). there was a tendency for the density of retinal ganglion cells to be larger in the central than in the peripheral retina. the diameter of retinal ganglion cells was 6-28 microns (mean: 1 ... | 1991 | 1950830 |
| the corticospinal tract attains a normal configuration in the absence of myelin: observations in jimpy mutant mice. | the corticospinal projection was examined in dysmyelinated, jimpy mice and in unaffected littermates following cortical injections of either wheat germ agglutinin conjugated to horseradish peroxidase or biocytin. corticospinal axons in both phenotypes traverse the medulla within a well-defined pyramidal tract, decussate within several fascicles at the spinomedullary junction, and extend down the spinal cord in a compact bundle in the ventral-most part of the dorsal funiculus. very few labeled fi ... | 1991 | 1908251 |
| cell movements during the initial phase of gastrulation in the sea urchin embryo. | the morphogenetic processes responsible for the initial phase of gastrulation in sea urchin embryos are not known. here we report observations of the size and position of clones of cells derived from horseradish peroxidase (hrp)-injected mesomeres and macromeres. the displacement of these clones during the initial phase of gastrulation suggests that involution is a mechanism involved in primary invagination. experiments with embryos marked with vital dyes indicate that movements occur only durin ... | 1991 | 1864470 |
| antigen recognition in the female reproductive tract. ii. endocytosis of horseradish peroxidase by langerhans cells in murine vaginal epithelium. | previous studies have shown that dendritic cells in the murine vaginal epithelium at diestrus and metestrus can endocytose intravaginally administered soluble protein tracers, but the identity of the dendritic cells was not established. in the investigation reported here, we used a combination of histochemistry and transmission electron microscopy to study the endocytosis of exogenous horseradish peroxidase by vaginal dendritic cells and to identify these cells as langerhans' cells on the basis ... | 1991 | 1786291 |
| modeling of immunosensors under nonequilibrium conditions. ii. experimental determination of performance characteristics. | in an attempt to optimize immunosensors operating with an immobilized antibody as binding protein and an analyte-enzyme conjugate as signal generator that is significantly larger in molecular size than the analyte, in a previous communication (part i) (s.-h. paek and w. schramm (1991) anal. biochem. 196) we developed mathematical models for the prediction of performance characteristics. these models are compared in this contribution with experimentally obtained results. as an example, a monoclon ... | 1991 | 1776682 |
| modeling of immunosensors under nonequilibrium conditions. i. mathematic modeling of performance characteristics. | immunosensors for the detection of small analytes that use analyte-enzyme conjugates as signal generators require special attention if operated under nonequilibrium conditions. if the size of the analyte and the analyte-enzyme conjugate differ substantially, the two antigens do not diffuse at the same rate. this can cause time-dependent shifts in the sensitivity of competitive immunoassays. therefore, immunosensors operating at short incubation times require precise timing that meets closely the ... | 1991 | 1776681 |
| temperature neurons in the crotaline trigeminal ganglia. | 1. intrasomal recordings were made with microelectrodes from 153 warm (infrared) neurons in the trigeminal ganglia of 36 crotaline snakes, trimeresurus flavoviridis. background discharges were observed at room temperature. the 153 warm neurons were classified into two groups: 81 were sensitive to less than or equal to 10 mg of von frey hair mechanical stimulation (warm t + m neuron), and 72 were insensitive to up to 100 mg or more of mechanical stimulation (warm t neuron). for t + m and t neuron ... | 1991 | 1774590 |
| projection of the retinal ganglion cells to the tectum differentiated from the prosencephalon. | the alar plate of the prosencephalon differentiates into a tectum-like structure when transplanted into the mesencephalon around the 10-somite stage. here, we report on the projection pattern of the retinal ganglion cells to the transplants. optic nerve fibers were labeled with horseradish peroxidase (hrp) and 3h-proline, and the innervation of the optic nerve fibers to the chimeric tectum was analyzed by hrp histochemistry on whole-mounted specimens, by autoradiography and by electron microscop ... | 1991 | 1661870 |
| oxygen diffusion near the heme binding site of horseradish peroxidase. | the quenching by molecular oxygen of the fluorescence of several probes complexed to apohorseradish peroxidase has been studied by intensity and time-resolved fluorescence methods. the probes utilized include 1-anilino-8-naphthalene sulfonic acid, 4,4'-bis (1-anilino-8-naphthalene sulfonic acid), and 2-p-toluidinylnaphthalene-6-sulfonic acid. these results are contrasted to those obtained using apohorseradish peroxidase complexed with protoporphyrin ix. the resistance of these complexes to denat ... | 1991 | 2069551 |
| carbachol-activated muscarinic (m1 and m3) receptors transfected into chinese hamster ovary cells inhibit trafficking of endosomes. | we examined the effects of isoproterenol and carbachol on fluid-phase endocytosis by chinese hamster ovary (cho) cells transfected with beta-adrenergic, m1, or m3 cholinergic receptors. isoproterenol increased camp production and carbachol increased intracellular ca, indicating successful expression of the receptor genes and coupling to typical signal transduction pathways. carbachol inhibited the uptake of horseradish peroxidase (hrp) or lucifer yellow (markers of fluid-phase endocytosis) in bo ... | 1991 | 1906173 |
| stereotyped pathway selection by growth cones of early epiphysial neurons in the embryonic zebrafish. | in this report we have examined the development of one of the earliest projections in the embryonic zebrafish brain, that from the epiphysis. epiphysial axons and growth cones were labelled anterogradely in whole-mounted brains, using either the carbocyanine dye, dii, or horseradish peroxidase (hrp). some embryos were also either stained with anti-acetylated tubulin or hnk-1 antibodies to reveal other axons in the brain, or were secondarily sectioned for light and electron microscopy. the epiphy ... | 1991 | 1935687 |
| tunicamycin-inhibited carrot somatic embryogenesis can be restored by secreted cationic peroxidase isoenzymes. | somatic embryogenesis of carrot (daucus carota l.) is inhibited by the glycosylation inhibitor tunicamycin. this inhibition is reversible by the addition of correctly glycosylated glycoproteins which have been secreted into the culture medium. to identify the proteins responsible for complementation, glycoproteins present in the medium of embryo cultures were purified and tested for their activity in the tunicamycin inhibition/ complementation assay. a 38-kda glycoprotein was purified that could ... | 1991 | 24194238 |
| auditory pontine grey: connections and response properties in the horseshoe bat. | this study investigates the role of the pontine grey as a link between the auditory system and the cerebellum in the bat, rhinolophus rouxi. we recorded response properties of single neurons in the pontine grey and, in the same preparation, injected wheat germ agglutinin - horseradish peroxidase (wga - hrp) in areas responsive to sound. thus the functional evidence was correlated with retrograde and anterograde transport. the main results are: (i) all auditory neurons in the pontine grey are tun ... | 1991 | 12106473 |
| products of estradiol/peroxidase interaction; their structural features and biopolymeric character. | loss of tritium from [2,4,6 alpha, 7 alpha-3h]estradiol and from [2-3h]estradiol during their conversion into polyestradiol (pel) by horseradish peroxidase/h2o2 and the nmr spectrum of pel permethyl ether suggest that pel is composed of two or more different subunits, each formed by the joining of four molecules of estradiol with the loss of five hydrogen atoms from positions 2 and 4 and of three phenolic hydrogens leading to the formation of one c-c bond and three c-o bonds. at very low concent ... | 1991 | 2069868 |
| 2d nmr of paramagnetic metalloenzymes: cyanide-inhibited horseradish peroxidase. | two-dimensional (2d) proton nmr correlation spectroscopy, cosy, and nuclear overhauser spectroscopy, noesy, have been used to explore the applicability of these methods for the moderately large (42 kda), paramagnetic cyanide-inhibited derivative of horseradish peroxidase, hrp-cn. the target resonances are those in the active site of hrp-cn which experience substantial hyperfine shifts and paramagnetic relaxation. the magnitude cosy experiment was found to yield cross peaks for all known spin-cou ... | 1991 | 1841693 |
| [physical separation of the populations of cochlear and vestibular efferent somas. a study using horseradish peroxidase]. | we have physically separated cochlear and vestibular end organs, and after injecting horseradish peroxidase two different populations of efferent somas have been described belonging to the cochlea and vestibule. | 2015 | 1742064 |
| link between cell apical morphology and h+ secretion in salamander small intestine. | to determine the morphological basis for the cellular secretion of h+ by in vitro salamander jejunum the transepithelial proton flux (jh) was measured under different conditions and compared with the ultrastructure of the villus absorptive cells using transmission electron microscopy in conjunction with morphometric analysis. norepinephrine (ne) stimulated jh and significantly increased the density of small cytoplasmic vesicular and tubular profiles in the region immediately below the apical pla ... | 1991 | 1650141 |
| purification of glycoproteins by selective transport using concanavalin-mediated reverse micellar extraction. | a novel methodology for coupling liquid-liquid extraction with affinity interaction has been developed to selectively and efficiently purify and separate glycoproteins. the basis for the separation is the selective extraction of glycoproteins from an aqueous solution into a reverse micellar organic phase by using concanavalin a (a sugar-binding lectin) as a facilitative carrier. specifically, horseradish peroxidase (a common glycoprotein) can be bound to concanavalin a in an aqueous phase and th ... | 2009 | 1370045 |
| block copolymer microdomains: a novel medium for enzymatic reactions. | block copolymers exhibit the phenomenon of microdomain formation in pure states as well as in solutions. the microdomains vest the block copolymer assemblies with the intriguing characteristics of microheterogeneous media. we demonstrate that this microheterogeneity in hydrophobic-hydrophilic block copolymer systems can be exploited for immobilizing enzymes and to carry out enzymatic reactions. examples involving cholesterol oxidase and horseradish peroxidase are provided here. the observed chan ... | 2016 | 1367345 |
| blood-nerve barrier in the frog during wallerian degeneration: are axons necessary for maintenance of barrier function? | blood-nerve barrier tissues (endoneurial blood vessels and perineurium) of the frog's sciatic nerve were studied during chronic wallerian degeneration to determine whether barrier function depends on the presence of intact axons. sciatic nerves of adult frogs were transected in the abdominal cavity; the ends were tied to prevent regeneration and the distal nerve stumps were examined. vascular permeabilities to horseradish peroxidase and to [14c]sucrose increased to day 14, returned toward normal ... | 1991 | 1865020 |
| musculotopic organization of the hypoglossal nucleus in the grass frog, rana pipiens. | recent neural tracer studies in several mammalian species have demonstrated a similar musculotopic organization of the hypoglossal motoneurons which innervate individual tongue muscles. the distribution of this musculotopic organization in nonmammalian tetrapods, however, has not received detailed investigation. as part of an ongoing study on the comparative organization of the vertebrate hypoglossal nucleus, the musculotopic organization of the hypoglossal nucleus of rana pipiens was studied by ... | 1991 | 1865014 |
| five treatment procedures evaluated for the elimination of ascorbate interference in the enzymatic determination of urinary oxalate. | we evaluated the efficacies of five treatment procedures for eliminating ascorbate interference in the enzymatic determination of urinary oxalate. aliquots of urine samples, containing different amounts of added ascorbate and oxalate, were individually subjected to ferric chloride, sodium nitrite, sodium periodate, charcoal, or ascorbate oxidase treatment to eliminate ascorbate interference. oxalate contents of the urine samples were then determined by a banana oxalate oxidase-horseradish peroxi ... | 1991 | 2049851 |
| rapid-freezing cytochemistry: preservation of tubular lysosomes and enzyme activity. | we show that tubular structures present in phorbol ester-stimulated macrophages are sensitive to commonly used chemical fixatives (i.e., they usually become fragmented during fixation). these structures are well preserved in macrophages that are physically fixed by rapid-freezing and subsequent freeze-substitution in osmium-acetone. we have developed methods that combine rapid-freezing, freeze-substitution, and enzyme cytochemistry for preservation of these tubular structures and for detection o ... | 1991 | 2033237 |
| one-electron oxidative activation of 2-aminofluorene by horseradish peroxidase compounds i and ii: spectral and kinetic studies. | horseradish peroxidase (hrp) catalyzed oxidation of the model carcinogen 2-aminofluorene (2-af) was studied by rapid scan spectral analysis and stopped flow kinetic measurements. our rapid scan spectral analysis of the changes in the enzyme oxidation states clearly indicates that the reactions between hrp intermediate compounds and 2-af are one-electron redox processes, thus a free radical is necessarily the primary product. the reactions were investigated at ph's ranging from 3.40 to 10.00. the ... | 1991 | 1898003 |
| primary afferent projections from the upper respiratory tract in the muskrat. | the central projections of the ethmoidal, glossopharyngeal, and superior laryngeal nerves were determined in the muskrat by use of the transganglionic transport of a mixture of horseradish peroxidase (hrp) and wheat germ agglutinin (wga)-hrp. the ethmoidal nerve projected to discrete areas in all subdivisions of the ipsilateral trigeminal sensory complex. reaction product was focused in ventromedial portions of the principal nucleus, subnucleus oralis, and subnucleus interpolaris. the subnucleus ... | 1991 | 1714922 |
| use of anti-horseradish peroxidase antibody-gold complex in the abc technique. | we report a modification of the avidin-biotin-peroxidase complex (abc) technique for the light and electron microscopic detection of antigens in tissue sections. an immunological approach was used instead of the dab reaction to reveal abc bound to antigen-antibody complexes. affinity-purified polyclonal antibodies against horseradish peroxidase were complexed to particles of colloidal gold and applied for reaction with the horseradish peroxidase molecules of the abc. for light microscopic immuno ... | 1991 | 1709659 |
| high-performance liquid chromatographic investigation of product formation in the horseradish peroxidase-enhanced chemiluminescence of luminol with different enhancers. | a reversed-phase high-performance liquid chromatographic method for the investigation of the chemiluminescence-producing oxidation of luminol by the enzyme horseradish peroxidase is presented. both the kinetics and the mechanism of product formation can be monitored. special attention is paid to the mechanism of enhancement of the chemiluminescence by phenolic compounds, such as p-iodophenol, p-hydroxycinnamic acid and 6-hydroxybenzothiazole. the function of the enhancers was elucidated partiall ... | 1991 | 1939457 |
| development and migration of avian sympathetic preganglionic neurons. | modern neuronanatomical techniques were used to investigate the development of the avian sympathetic preganglionic cell column in the spinal cord of the chick embryo. [3h]thymidine autoradiography indicated that the majority of these preganglionic, or "terni column" neurons are generated between stages 18 and 24 (days 2-4). this coincides with the genesis of the somatic motoneurons in the thoracic levels of the cord, and therefore differences in the time of origin cannot explain the divergent fa ... | 1991 | 1713232 |
| [a spectrophotometric method for determining the concentration of l-lysine using l-lysine-alpha-oxidase from trichoderma sp. and 3,3',5,5'-tetramethylbenzidene dihydrochloride]. | a simple and relatively sensitive procedure was developed for determination of l-lysine at 3-30 mmole/l concentration. the procedure does not involve the carcinogenic compound o-dianisidine. l-lysine alpha-oxidase catalyzed oxidative deamination of l-lysine with o2 consumption and formation of h2o2, nh3 and alpha-keto-epsilon-aminocaproic acid. horseradish peroxidase and a non-carcinogenic compound 3,3,5,5'-tetramethylbenzidine dihydrochloride as an electron donor were used in determination of h ... | 2015 | 1949695 |
| [a neuroanatomical study of the vestibular efferent system, iii: the origin and pathway of the axons]. | we have studied the vestibular efferent fibers pathway, after scratching the peripheral vestibular receptors and horseradish peroxidase injection, and tmb or dab processing. we have proved the existence of a differentiated bundle in the main vestibular pathway, this bundle crosses the areas where the efferent somas have been situated, some of this fibers have been seen crossing the midline. it has not been possible to connect the efferent somas with the fibers. | 2015 | 1907835 |
| preliminary studies on quinoprotein glucose dehydrogenase under extreme conditions of temperature and pressure. | the kinetics of the reduction of the quinoprotein glucose dehydrogenase by substrate were studied as a function of 3 parameters: pressure (1-1000 bar), temperature (down to -25 degrees c) and solvent (water and 40% dimethyl sulfoxide, dmso) using a high-pressure low-temperature stopped-flow apparatus. a 2-step formation of the reduced enzyme by its substrate (xylose), was observed. a rapid equilibrium described by the constant k1 was followed by a slower process described by the constants k2 and ... | 1991 | 1764506 |
| development of the laminar distribution of thalamocortical axons and corticothalamic cell bodies in the visual cortex of the wallaby. | the distribution of afferents from the dorsal lateral geniculate nucleus (lgnd) and the lateral posterior nucleus (lp) and of cell bodies projecting to these nuclei has been studied in the visual cortex of the wallaby (macropus eugenii) throughout development to determine how the characteristic laminar distribution of afferents and efferents of the mature cortex is achieved. young are born after 26-28 days of gestation and do not open their eyes until around 140 days after birth. horseradish per ... | 1991 | 1713226 |
| neurotransmitter organization of the nucleus of edinger-westphal and its projection to the avian ciliary ganglion. | two morphologically distinct types of preganglionic endings are observed in the avian ciliary ganglion: boutonal and cap-like. boutonal endings synapse on ciliary ganglion neurons (called choroidal neurons) innervating choroidal blood vessels, while cap-like endings synapse on ciliary ganglion neurons (called ciliary neurons) controlling the lens and pupil. some of both types of preganglionic endings contain the neuropeptides substance p (sp) and/or leucine-enkephalin (lenk). although both types ... | 1991 | 1712628 |
| luminography--a new, highly sensitive visualization method for electrophoresis. | a highly sensitive method for protein visualization following electrophoresis and protein blotting was developed. the method is based on the light-emitting reaction of luminol and hydrogen peroxide catalyzed by horseradish peroxidase. the luminescent assay can be applied either to the native gel or after protein blotting, and it has a sensitivity two orders of magnitude higher than that achieved with chromogenic detection systems. analogous to autoradiography the luminescent signal is recorded o ... | 1991 | 1682145 |
| regular mosaics of large displaced and non-displaced ganglion cells in the retina of a cichlid fish. | large retinal ganglion cells in the tilapid cichlid fish oreochromis spilurus (standard length 15-54 mm) were filled with horseradish peroxidase and studied in flatmounts. three types, with distinct patterns of dendritic stratification, formed spatially independent, nonrandom mosaics. one type (about 0.3% of all ganglion cells) resembled the outer (off) alpha cells of mammals. they were very large, with thick primary dendrites and large, sparsely branched planar trees in the outer part of the in ... | 1991 | 2071699 |
| differential innervation patterns of three divisions of frog auditory midbrain (torus semicircularis). | the connectivity pattern of the laminar, principal, and magnocellular nuclei of the frog torus semicircularis (ts) was investigated. a small amount of horseradish peroxidase was injected focally into individual divisions of the ts and anterograde and retrograde transport patterns were observed. results of our tracing study showed that these divisions of the ts possessed distinct innervation patterns. the principal nucleus appeared to be the primary input port of the ts receiving extensive inputs ... | 1991 | 1712796 |
| axonal regeneration in the adult lamprey spinal cord. | larval sea lampreys recover from complete spinal transection by a process involving directionally specific axonal regeneration. in order to determine whether this is also true of adults, 14 adult lampreys were transected at the level of the 5th gill and allowed to recover for 10 weeks. müller and mauthner cells and their giant reticulospinal axons (gras) were impaled with microelectrodes and injected with horseradish peroxidase (hrp). the tissue was processed for hrp histochemistry and wholemoun ... | 1991 | 1865001 |
| the edinger-westphal nucleus: sources of input influencing accommodation, pupilloconstriction, and choroidal blood flow. | this study used neuroanatomical techniques to investigate sources of afferents to the edinger-westphal nucleus (ew) of the pigeon. the ew contains the parasympathetic preganglionic neurons that, by way of the oculomotor nerve, project to the ciliary ganglion (narayanan and narayanan, '76; lyman and mugnaini, '80). the ciliary ganglion, in turn, innervates the internal musculature of the eye; the ciliary body, the iris sphincter muscle, and the smooth muscle of choroidal blood vessels (marwitt et ... | 1991 | 1713924 |
| microtubule-disrupting drugs blocked delivery of endocytosed transferrin to the cytocenter, but did not affect return of transferrin to plasma membrane. | the fluorescence of fl cells after endocytosis of rhodamine-labeled transferrin initially appeared as a dispersed punctate pattern over the whole cell and then accumulated in the cytocenter on further incubation. in nocodazole-treated cells, the punctate fluorescence appeared along the cell edges, and stayed there on further incubation but did not accumulate in the cytocenter. the localization of transferrin was examined at the electron microscopic level with horseradish peroxidase (hrp)-labeled ... | 1991 | 1869507 |
| distribution of glycoconjugates in ion transport cells of gerbil inner ear. | ion transport cells in gerbil inner ear were differentiated histochemically by staining glycoconjugates (gcs) with a battery of horseradish peroxidase-conjugated lectins. strong staining with psa and lca showed a high content of n-linked oligosaccharides in transport cell gcs. reactivity with pha-l and pha-e identified gc with triantennary and with bisected biantennary n-linked oligosaccharides, respectively, in these cells. high affinity for dsa and pwm demonstrated abundant n-acetyl lactosamin ... | 1991 | 1848571 |
| an interplexiform cell in the goldfish retina: light-evoked response pattern and intracellular staining with horseradish peroxidase. | the light-evoked response pattern and morphology of one interplexiform cell were studied in the goldfish retina by intracellular recording and staining. the membrane potential of the cell spontaneously oscillated in the dark. in response to a brief light stimulus, the membrane potential initially gave a slow transient depolarization. during maintained light, the oscillations showed a tendency to be suppressed; the response of the cell to the offset of the stimulus was not so prominent. the perik ... | 1991 | 1711416 |
| morphophysiology of synaptic transmission between type i hair cells and vestibular primary afferents. an intracellular study employing horseradish peroxidase in the lizard, calotes versicolor. | intracellular records with glass microelectrodes filled with horseradish peroxidase (hrp) were taken from primary afferents of the horizontal semicircular canal in the lizard, calotes versicolor. a coefficient of variation (cv) of the interspike intervals of spontaneous action potentials (aps) was calculated and correlated with the terminal morphologies of afferents within the canal crista. irregular fibers with cv greater than 0.4 always correlated with a nerve chalice or calyx afferent termina ... | 1991 | 1713111 |
| differential projections of the main and accessory olfactory bulb in the frog. | the central projections of the main olfactory bulb and the accessory olfactory bulb of the adult leopard frog (rana pipiens) were reexamined, by using a horseradish peroxidase anterograde tracing method that fills axons with a continuous deposit of reaction product. the fine morphology preserved by this method allowed the terminal fields of the projection tracts to be delineated reliably, and for the first time. herrick's amygdala has been newly subdivided into cortical and medial nuclei on the ... | 1991 | 1709955 |
| a note on the organization of the amphibian olfactory bulb. | after horseradish peroxidase (hrp) injections were made in limited sectors of the main olfactory bulb in the adult frog rana pipiens, the cellular morphology of mitral cells and granule cells impregnated with hrp were examined in uninjected regions of the bulb. mitral cells were observed to possess glomerular dendrites and prominent secondary dendrites, both of which have smooth shafts. the glomerular dendrites may be multiple, are often branched, and may arise from secondary dendrites, as well ... | 1991 | 1709954 |
| targets of horizontal connections in macaque primary visual cortex. | pyramidal neurons within the cerebral cortex are known to make long-range horizontal connections via an extensive axonal collateral system. the synaptic characteristics and specificities of these connections were studied at the ultrastructural level. two superficial layer pyramidal cells in the primate striate cortex were labeled by intracellular injections with horseradish peroxidase (hrp) and their axon terminals were subsequently examined with the technique of electron microscopic (em) serial ... | 1991 | 1709953 |
| reciprocal connections between the 'nucleus rotundus' and the dorsal lateral telencephalon in the weakly electric fish gnathonemus petersii. | injections of horseradish peroxidase into the dorsal lateral telencephalon of the weakly electric mormyrid fish gnathonemus petersii resulted in retrogradely filled cells and anterogradely labelled terminals in the 'nucleus rotundus' of the ipsilateral rostral diencephalon. this connection courses via the lateral part of the lateral forebrain bundle. the present results suggest a particularly close relationship between the dla cell group of the lateral telencephalon and the 'nucleus rotundus'. | 1991 | 1711403 |
| afferent and efferent connections of the olfactory bulbs in the lizard podarcis hispanica. | the connections of the olfactory bulbs of podarcis hispanica were studied by tract-tracing of injected horseradish peroxidase. restricted injections into the main olfactory bulb (mob) resulted in bilateral terminallike labeling in the medial part of the anterior olfactory nucleus (aon) and in the rostral septum, lateral cortex, nucleus of the lateral olfactory tract, and ventrolateral amygdaloid nucleus. bilateral retrograde labeling was found in the rostral lateral cortex and in the medial and ... | 1991 | 1709182 |
| rapid typing of dna sequence polymorphism at the hla-drb1 locus using the polymerase chain reaction and nonradioactive oligonucleotide probes. | an hla-dr typing system that uses sequence-specific oligonucleotide (sso) probes conjugated to horseradish peroxidase (hrp) for analyzing drb alleles amplified by the polymerase chain reaction has been developed. using 25 hrp-sso probes and two primer pairs for generic and for drb1 locus-specific amplification, we can distinguish 31 of 34 hla-drb1 alleles. this procedure is suitable for typing heterozygous samples from a variety of sources, including cdna templates, and can detect in a simple an ... | 1991 | 2055783 |
| compensation for population size mismatches in the hamster retinotectal system: alterations in the organization of retinal projections. | unilateral partial ablation of the superior colliculus in the hamster results in a compression of the retinotopic map onto the remaining tectal fragment. in a previous electrophysiological study (pallas & finlay, 1989a), we demonstrated that receptive-field properties of single tectal units (including receptive-field size) remain unchanged, despite the increased afferent/target convergence ratios in the compressed tecta. the present study was done to investigate the mechanism that produces incre ... | 1991 | 2054328 |
| alterations in sugar residues in squamous metaplasia in hamster tracheal explants induced by benzo(a)pyrene and its reversal by retinoic acid. | we have demonstrated that squamous metaplasia induced by benzo(a)pyrene (bp) in the hamster tracheal explants accompany distinct alterations in carbohydrate moieties in the epithelial mucosa. most prominent alterations were the preferential binding of peanut agglutinin (pna) and wheat germ agglutinin (wga) in the basal cell layer in metaplastic lesions. in this study we examined if reversal of bp-induced lesions by all-trans retinoic acid (ra) results in the acquisition of normal carbohydrate co ... | 1991 | 2033021 |
| using fluorescence photoablation to study the regeneration of singly cut leech axons. | the regeneration of the axons of leech retzius cells was compared following two different methods of axonal severing: (1) a crush of the whole connective that includes the retzius axon; and (2) photoablation of a small segment of only the retzius axon. the photoablation was carried out after filling the retzius cell with lucifer yellow (ly). several tests were carried out to determine whether the photoablation actually severed the axon. these included (1) using the lipophilic membrane probe dii ... | 1991 | 2030337 |
| an oligopeptide permeates intestinal tight junctions at glucose-elicited dilatations. implications for oligopeptide absorption. | turnover of the na(+)-glucose cotransporter in the apical membrane of intestinal absorptive cells elicits alterations in tight-junction structure including the appearance of intrajunctional dilatations. paralleling these structural responses, epithelial permeability to ions and nutrient-sized solutes increases. however, it is not known how these observed permeability changes specifically relate to the structural alterations elicited by glucose. using a hemeconjugated peptide tracer (mp-11; mol w ... | 1991 | 1993492 |
| model systems for metabolism studies. biomimetic oxidation of acetaminophen and ellipticine derivatives with water-soluble metalloporphyrins associated to potassium monopersulfate. | some original water-soluble metalloporphyrins/khso5 systems were developed to mimic the metabolic biooxidation of drugs. oxidation of acetaminophen and various ellipticine derivatives were used as model reactions. oxidative products (mainly quinone-imine structures) were obtained in good yield after 2 min of reaction, for a catalyst/substrate ratio of 0.04. iron(iii) derivative of tetrasodium meso-tetrakis(p-sulfonatophenyl)porphyrin and manganese(iii) derivative of tetraacetate meso-tetrakis(4- ... | 2013 | 1676637 |
| reactions of hydrated electrons with horseradish peroxidase: a pulse radiolysis study. | kinetics of the reactions of hydrated electrons with horseradish peroxidase (hrp) have been studied by pulse radiolysis. hydrated electrons reduce hrp to its ferrous form with a yield of 40%. at higher dose (250 gy) the formation of compound i followed by its reduction to compound ii was also observed. | 1991 | 1672352 |
| mechanism of hydrogen peroxide formation catalyzed by nadph oxidase in thyroid plasma membrane. | the thyroid plasma membrane contains a ca2(+)-regulated nadph-dependent h2o2 generating system which provides h2o2 for the thyroid peroxidase-catalyzed biosynthesis of thyroid hormones. the plasma membrane fraction contains a ca2(+)-independent cytochrome c reductase activity which is not inhibited by superoxide dismutase. but it is not known whether h2o2 is produced directly from molecular oxygen (o2) or formed via dismutation of super-oxide anion (o2-). indirect evidence from electron scavenge ... | 1991 | 1995628 |
| aspartate-like immunoreactivity in vestibulospinal neurons in gerbils. | in an effort to further characterize vestibulospinal pathways in the gerbil, immunocytochemistry was combined with retrograde identification of neurons. vestibulospinal neurons were retrogradely labeled following injections of horseradish peroxidase into the cervical cord of anesthetized gerbils. sections were reacted with nickel acetate-diaminobenzidine for horseradish peroxidase, giving a black reaction product. sections were incubated in polyclonal antisera to aspartate, incubated in an avidi ... | 1991 | 1709271 |
| iodoacetate action on endocytic uptake of different fluid-phase markers by ok renal epithelial cells. | when grown in monolayer culture, ok cells display endocytic uptake of soluble fluid-phase markers such as lucifer yellow (ly) and horseradish peroxidase (hrp). the response of this process to metabolic inhibitors was characterized in the present study. inhibition of cell metabolism by cyanide produced a decrease in cell atp content which was accompanied by a decrease in uptake of both ly and hrp, confirming the energy-dependence of fluid-phase endocytosis in ok cells. use of iodoacetate also dec ... | 1991 | 2001415 |
| the cdna sequence of a neutral horseradish peroxidase. | a cdna clone encoding a horseradish (armoracia rusticana) peroxidase has been isolated and characterized. the cdna contains 1378 nucleotides excluding the poly(a) tail and the deduced protein contains 327 amino acids which includes a 28 amino acid leader sequence. the predicted amino acid sequence is nine amino acids shorter than the major isoenzyme belonging to the horseradish peroxidase c group (hrp-c) and the sequence shows 53.7% identity with this isoenzyme. the described clone encodes nine ... | 1991 | 2001399 |
| a novel mannose-specific and sugar specifically aggregatable lectin from the bark of the japanese pagoda tree (sophora japonica). | a new d-mannose/d-glucose-specific lectin (b-sja-ii) was isolated from the bark of the japanese pagoda tree, sophora japonica. b-sja-ii was separated from a well known d-galactose/n-acetyl-d-galactosamine-specific lectin (b-sja-i) by affinity chromatography on lactamyl-sepharose, then purified by affinity chromatography on maltamyl-sepharose. on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, b-sja-ii gave four bands: subunit a-1 (mr = 19,400), a-2 (mr = 18,200), b-1 (mr = 15,000), an ... | 1991 | 1993686 |
| hla-dr, dq and dp typing using pcr amplification and immobilized probes. | a simple, rapid, and precise method of typing hla class ii polymorphism would be valuable in the areas of disease susceptibility, tissue transplantation, individual identification and anthropological genetics. here we describe a method of analysing class ii sequence polymorphism based on polymerase chain reaction (pcr) amplification and hybridization with oligonucleotide probes. one valuable property of sequence-based hla typing strategies, like oligonucleotide probe hybridization, is that they ... | 2004 | 2069944 |
| ultrastructural study of the avian ventral lateral geniculate nucleus. | the ultrastructure of the pigeon and quail ventral lateral geniculate nucleus was analyzed with standard electron microscopy and horseradish peroxidase tracing of its retinal and tectal afferents. six types of neurons were distinguished: two large, two medium-sized, and two small types. the latter do not project to the optic tectum and appear to be interneurons. large and medium-sized neurons project to the optic tectum and are thus relay neurons. profiles with round, large synaptic vesicles wer ... | 1991 | 2049329 |
| inactivation of aminated horseradish peroxidase by interaction with s-sepharose. | horseradish peroxidase which had been aminated by periodate oxidation and reductive amination was purified by cation-exchange chromatography on s-sepharose. instead of the expected single peak of aminated enzyme, two distinct peaks of protein were eluted from the column. evaluation of the protein in each of the two distributions showed that peak number 1 had spectral properties and specific activity similar to those of native enzyme. distribution number 2 had a threefold reduction in the extinct ... | 1991 | 2035845 |
| pathfinding by growth cones of commissural interneurons in the chick embryo spinal cord: a light and electron microscopic study. | to investigate putative axonal guidance mechanisms used by commissural interneurons in the chick embryo spinal cord, we have examined growth cone morphology, the microenvironment through which the growth cones advance, and interactions between growth cones and their surroundings. growth cones of both early and late developing commissural interneurons were examined. the growth cones were visualized by injection of either horseradish peroxidase (hrp) or the fluorescent dye di-i. unlabelled growth ... | 1991 | 2016414 |
| variation in terminal morphology and presynaptic inhibition at crustacean neuromuscular junctions. | synaptic terminals of excitatory and inhibitory neurons supplying muscle fibers in leg muscles of crabs (pachygrapsus crassipes and hyas areneus) were investigated with light and electron microscopy. terminals responsible for large excitatory postsynaptic potentials (epsps) at low frequencies of activation had a compact configuration with clusters of terminal boutons radiating from the main axon branch. terminals responsible for small epsps had a more diffuse organization, with boutons often arr ... | 1991 | 2016409 |
| conformational relaxation of a low-temperature protein as probed by photochemical hole burning. horseradish peroxidase. | for the first time, conformational relaxation processes have been measured in a small protein, mesoporphyrin-horseradish peroxidase via their influence on spectral diffusion broadening of holes burnt in the fluorescence excitation spectrum of free base mesoporphyrin. holes were burnt in three 0----0 bands of different tautomeric forms of the chromophore at 1.5 and 4 k, and the spectral diffusion broadening was measured in temperature cycling experiments between 4 and 30 k. the inhomogeneous line ... | 1991 | 2009354 |
| intracellular recordings from gallbladder ganglia of opossums. | postganglionic parasympathetic neurons of the opossum gallbladder were studied using morphological and intracellular electrophysiological recording techniques. on average there were 17 ganglia/cm2 with 8 neurons/ganglion arranged as loosely or densely packed clusters. intracellular injection of horseradish peroxidase identified two types of neurons. most of the neurons (approximately 80%) had a single long process that extended beyond the ganglion border with few or no short additional neurites. ... | 1991 | 1996648 |
| [endothelial vesicles as a transport system in the blood-brain barrier. morphometric study during development]. | the vesicles and vacuoles of the endothelia, morphological expression of endocytosis and transendothelial transport, are quite absent in the mature neural endothelia. in order to study the temporal sequence of the vesicle and vacuole modifications during the blood brain barrier (bbb) setting up, the extent of these structures was morphometrically analyzed on electron micrographs of neural microvessels in the optic tectum of 8, 14, and 17 day chick embryos, fixed after an intracardial injection o ... | 1991 | 1888483 |
| heme-protein fission under nondenaturing conditions. | slow heme transfer from horseradish peroxidases c2 and a2, cytochrome c peroxidase, chloroperoxidase, and leghemoglobins to a heme acceptor protein, apomyoglobin, has been studied under mild conditions. the reaction is best described as heme release into water followed by quick engulfment by apomyoglobin. the energetics of the activated process are large and interpreted as connected to both polypeptide motions during release and the ordering of water around the heme during solvation. the free en ... | 1991 | 1846966 |
| resonance raman investigation of ferric iron in horseradish peroxidase and its aromatic donor complexes at room and low temperatures. | resonance raman (rr) spectra of the acidic form of feiii horseradish peroxidase (hrp) were obtained at room and low temperatures using b- and q-band excitation. at 296 k, hrp exhibits two sets of porphyrin skeletal stretching frequencies which are attributed to a thermal mixture of 5- and 6-coordinate high-spin feiii states. when the temperature is lowered, the observed bands shift to higher frequencies, and these are assigned to intermediate- and low-spin states. addition of 40% glycerol has no ... | 1991 | 1988064 |
| changes in size and number of spinal motoneurons in relation to growth of the musculature in the eel, anguilla. | estimates of the numbers of spinal motoneurones in relation to growth in the eel, anguilla, were made by counting the axons contained within the ventral roots. motoneuronal size was determined as the surface area of cell somata labelled retrogradely from applications of tracer (horseradish peroxidase, cobalt lysine) to the musculature or spinal nerves. the sizes and numbers of muscle fibres from red and white portions of the myotomal musculature were determined from frozen sections. measurements ... | 1991 | 2015656 |
| citric acid-ammonium acetate buffer. | a ph table is reported for citric acid-ammonium acetate buffers that are useful for horseradish peroxidase (hrp) histochemistry. | 1991 | 1888793 |
| uptake and accumulation of ajmalicine into isolated vacuoles of cultured cells of catharanthus roseus (l.) g. don. and its conversion into serpentine. | isolated vacuoles from ajmalicine-producing cell suspensions of catharanthus roseus accumulated the alkaloid ajmalicine. dissipation of the transtonoplast ph gradient with nigericin abolished ajmalicine accumulation, whereas dissipation of the transtonoplast potential with valinomycin had no effect. addition of mg-atp resulted in a higher ajmalicine accumulation. serpentine produced by the cells was largely recovered in isolated vacuoles; in contrast, ajmalicine was lost. ajmalicine was converte ... | 1991 | 24193617 |
| ultrastructure of the pronephric kidney of embryos and prolarvae of the sea lamprey, petromyzon marinus. | embryos of lampreys petromyzon marinus were obtained through a technique of artificial fertilization. samples of developmental intervals to the prolarval stage were prepared for transmission electron microscopy and the pronephros was examined. the pronephros was visible in the cardiac region of the coelom prior to the time of hatching of embryos and consisted of a renal corpuscle, nephrostomes, and proximal tubules connected to a pronephric duct. the renal corpuscle was comprised of poorly-defin ... | 1991 | 18621169 |
| nonuniform variation in band pattern with luminol/horseradish peroxidase western blotting. | when optimized, the peroxidase-catalyzed oxidation of luminol can provide a useful, sensitive detection system for western blotting. however, while the luminescence from intense bands appears rapidly, faint bands require at least 30 min after removal of the membrane from reaction buffer for maximum luminescence to develop. this can result in the detection of a variant band pattern if films are exposed to the blotted membrane too soon after reaction, while exposure later after reaction can result ... | 1991 | 2048735 |
| the primary projections of the lateral-line nerves of the florida gar, lepisosteus platyrhincus. | gars, like most other ray-finned fishes, possess lines of superficial and canal neuromasts that form highly ordered spatial arrays on the head and trunk. these neuromasts are innervated by three pairs of cranial nerves: anterior, middle and posterior lateral-line nerves. application of horseradish peroxidase to the roots of these nerves indicates that the afferent fibers of each nerve terminate throughout the rostrocaudal extent of the ipsilateral octavolateralis column, composed of medial and c ... | 1991 | 2029608 |
| rapid typing of hla-dqb1 dna polymorphism using nonradioactive oligonucleotide probes and amplified dna. | the allelic sequence diversity at the hla-dqb1 locus has been analyzed by polymerase chain reaction (pcr) amplification and sequencing. fifteen amino acid sequence-defined alleles (one previously unreported) and several silent nucleotide polymorphisms which subdivide these alleles have been identified. here, we describe the specific amplification of the dqb1 second exon by several different pcr primer pairs and a simple and rapid typing procedure using a panel of 16 horseradish peroxidase (hrp)- ... | 1991 | 2010218 |
| quantitative determination of different apolipoprotein b containing lipoproteins by an enzyme linked immunosorbent assay: apo b with apo c-iii and apo b with apo e. | a non competitive enzyme-linked immunosorbent assay (elisa) for total apolipoprotein (apo) b, apo b with apo c-iii (lpc-iii:b), and apo b with apo e (lpe:b), was developed. microtiter plates were used as solid-phase, and subdivided into three parts, coated respectively with affinity purified antibodies to apo b, to apo c-iii and to apo e. after incubating the antigen with coated plates, a horseradish peroxidase-labelled antibody to apo b was added to all the plates to estimate total apo b, lpc-i ... | 1991 | 1939663 |
| motor neurons and motor columns of the anterior spinal cord of salamanders: posthatching development and phylogenetic distribution. | the posthatching development of rostral (1st-4th) spinal motor neurons was studied in ten species of salamanders, using horseradish peroxidase and cobaltic lysine tracing techniques. development of spinal motor neurons differs among species in association with differences in life history and general developmental patterns (i.e., between species with aquatic larvae versus those with direct development, with or without ontogenetic repatterning). in the plesiomorphic state, represented by species w ... | 1991 | 1913139 |
| retinofugal and retinopetal projections in the green sunfish, lepomis cyanellus. | the retinofugal and retinopetal connections in the green sunfish were studied by autoradiographic and horseradish peroxidase methods. all retinofugal fibers decussate in the optic chiasm. some fibers project to contralateral preoptic and hypothalamic nuclei while others recross to project to the comparable ipsilateral nuclei. contralaterally, the medial optic tract projects to the periventricular thalamic and pretectal nuclei and, sparsely, to the rostral optic tectum. the dorsal optic tract pro ... | 1991 | 1913138 |
| population study of horizontal cells in live carp retinas using microinjections of horseradish peroxidase. | in order to randomly sample horizontal cells (hcs) of carp retina, horseradish peroxidase (hrp) was delivered into axon terminals by means of vitreally inserted micropipettes in live fish. survival time was extended to several hours to permit label diffusion into hc somata. a function measuring the relative complexity of cell contours was defined in order to help classify hcs according to their morphological types. the histogram of measurement from 313 hcs showed three modes fitting normal distr ... | 1991 | 1891806 |
| examination of neurons in wild type and mutants of caenorhabditis elegans using antibodies to horseradish peroxidase. | antibodies to horseradish peroxidase (hrp) recognize 27 of 302 neurons and several non-neuronal cells in adult hermaphrodites of the soil nematode caenorhabditis elegans and can be used to label these cells for cytological analysis in whole animals. the antibodies bind to the anterior members, but not to the posterior members of a set of mechanosensory neurons in wild type animals. binding to one of the posterior mechanosensory neurons (pvm) occurs when this neuron migrates to an abnormal anteri ... | 1991 | 1886035 |
| the peroxidase-catalyzed oxidation of kyotorphins. | in vitro experiments are reported showing that the dipeptides tyr-l-arg (kyotorphin) and tyr-d-arg (d-arg-kyotorphin) can be oxidized by h2o2-horseradish peroxidase system: the products formed are characterized by absorption spectra with two peaks at 290 nm and 315 nm. the effects of substrate and enzyme concentration on the oxidation rate are described. amino acid analysis of hydrolysates of peroxidase-treated kyotorphins provides evidence for the presence of dityrosine. the data suggest that t ... | 1991 | 1863276 |
| visual responses of morphologically identified tectal cells in the goldfish. | intrinsic cells from the optic tectum of the goldfish were recorded intracellularly while undergoing photic stimulation, injected iontophoretically with horseradish peroxidase (hrp), and subsequently recovered in sectioned material. unequivocal identifications were possible for 72 cells. the majority of these belonged to type i or type xiv, but types ii, iii, vi, ix and xiii were also observed. type i cells exhibited a characteristic response to light flash with a strong initial hyperpolarizatio ... | 1991 | 1843756 |
| ganglion cells in the goldfish retina: correlation of light-evoked response and morphology. | goldfish retinal ganglion cells were intracellularly stained with horseradish peroxidase after recording their responses to a predetermined set of test stimuli. depolarizing responses were elicited by cells differing in shapes and sizes of their somata and dendritic fields; these cells were mostly bistratified in the inner plexiform layer (sublamina b and distal sublamina a). hyperpolarizing responses were generated by cells monostratified in a, and by cells bistratified in a and at the a/b bord ... | 1991 | 1843754 |