| simultaneously tracing the geographical origin and presence of bovine milk in italian water buffalo mozzarella cheese using maldi-tof data of casein signature peptides. | water buffalo (wb) casein (cn) and curd samples from indigenous italian and international breeds were examined with the objective of identifying signature peptides that could function as an indicator to determine the origin of their milk products. cn in complex mixtures were digested with trypsin, and peptide fragments were subsequently identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (maldi-tof ms). the unique presence of a β-cn a variant and an interna ... | 2016 | 27299776 |
| assessment of foot health and animal welfare: clinical findings in 229 dairy mediterranean buffaloes (bubalus bubalis) affected by foot disorders. | lameness represents the third most important health-related cause of economic loss in the dairy industry after fertility and mastitis. although, dairy mediterranean buffaloes (mb) and dairy cows share similar breeding systems predisposing to similar herd problems, published studies exploring its relevance and role in these ruminants are still rare and incomplete. the aims of this study were to describe the clinical findings of foot disorders (fds) in dairy mb and their influence on animal welfar ... | 2016 | 27297174 |
| sister chromatid exchange test in river buffalo lymphocytes treated in vitro with furocoumarin extracts. | furocoumarin extracts from psoralea morisiana, the endemic sardinian legume species, were tested for their mutagenic potential on river buffalo blood cells. the results obtained performing the sister chromatid exchange (sce) test in blood cultures of five river buffalo calves (exposure to furocoumarins for 72h) and five cows (exposure to furocoumarins for 3h, in the absence and presence of s9 metabolic activator) are reported. significant differences in mean values of sces were observed in cells ... | 2016 | 27180332 |
| effect of mastitis on luteal function and pregnancy rates in buffaloes. | the aim of this study was to investigate the effects of mastitis on cl development and function and pregnancy rate in buffaloes. sixty-six buffaloes (bubalus bubalus) reared in a commercial farm at el-beheira governorate, north of egypt were used in this study. according to the visual observation of milk, physical examination of the udder and actual somatic cell count in milk, buffalo cows were divided into three groups: without mastitis (w), n = 23; subclinical mastitis (sc), n = 18; and clinic ... | 2016 | 27177967 |
| high genetic diversity and distribution of bubu-dqa alleles in swamp buffaloes (bubalus bubalis carabanesis): identification of new bubu-dqa loci and haplotypes. | in this study, genetic diversity analysis of mhc class ii-dqa locus helped in identification of 25 new bubu-dqa nucleotide sequences in swamp buffaloes (bubalus bubalis carabanesis, bubu). phylogenetic analysis revealed the distribution of the buffalo dqa sequences in two major clusters of dqa1 and dqa2 genes, sharing common lineages with corresponding cattle alleles, possibly due to trans-species evolution. however, a highly divergent sequence, bubu-dqa*2501, homologous to cattle (bola) dqa3 al ... | 2016 | 27177904 |
| polymorphism in the a2m gene associated with high-quality milk in murrah buffaloes (bubalus bubalis). | the study of genes associated with host defense mechanisms, such as the a2m gene, plays a critical role in preventing diseases that reduce milk yield and its constituents. the aim of this study was to identify polymorphisms in the a2m gene in murrah buffaloes (bubalus bubalis), and investigate their associations with milk yield, fat and protein production, fat and protein percentages, and somatic cell count. hair follicle samples of 136 animals were collected for dna extraction, and polymorphism ... | 2016 | 27173184 |
| expression of putative stem cell marker, hepatocyte nuclear factor 4 alpha, in mammary gland of water buffalo. | buffaloes account for more than 56% of total milk production in india. cyclic remodeling of mammary glands of human, mice, cow, sheep, and goat is determined by mammary stem cells. it is logical to assume that buffalo mammary gland will have mammary stem/progenitor cells. thus far, no report exists on identification of buffalo mammary stem cells. hepatocyte nuclear factor 4 alpha (hnf4a) is a candidate marker for hepatic progenitor cells and has recently been suggested as a marker of bovine mamm ... | 2016 | 27092988 |
| how livestock and flooding mediate the ecological integrity of working forests in amazon river floodplains. | the contribution of working forests to tropical conservation and development depends upon the maintenance of ecological integrity under ongoing land use. assessment of ecological integrity requires an understanding of the structure, composition, and function and major drivers that govern their variability. working forests in tropical river floodplains provide many goods and services, yet the data on the ecological processes that sustain these services is scant. in flooded forests of riverside am ... | 2016 | 27039519 |
| isolation and identification of prepubertal buffalo (bubalus bubalis) spermatogonial stem cells. | isolation and culture of spermatogonial stem cells (sscs) are attractive for production of genetic modified offspring. in the present study, buffalo spermatogonial stem-like cells were isolated, cultured and expression pattern of different germ cell marker genes were determined. to recover spermatogonia, testes from age 3 to 7 months of buffalo were decapsulated, and seminiferous tubules were enzymatically dissociated. two types of cells, immature sertoli cell and type a spermatogonia were obser ... | 2016 | 26954139 |
| sequence analysis of the s1pr1 gene in river buffalo. | recent developments in methodologies for genomic analyses have enabled a significant advance in understanding of the river buffalo genome. the s1pr1 gene has been mapped to buffalo chromosome 6 and bovine chromosome 3; this gene is of interest as it is a candidate for marbling in meat, an important economic trait. here, we performed next generation sequencing in a buffalo bac dna clone and obtained a 54.5-kb sequence encompassing the entire buffalo s1pr1 gene as well as the 27 kb upstream region ... | 2016 | 26909928 |
| investigation into omocysteine, vitamin e and malondialdehyde as indicators of successful artificial insemination in synchronized buffalo cows (bubalus bubalis). | the aim of this study was to describe modifications in plasma homocysteine (hcy), vitamin e (vite) and malondialdehyde (mda) concentrations in the first 56 days after artificial insemination (ai) in buffalo. thirty-five buffalo cows were divided, ex post, into three groups on the basis of pregnancy diagnosis: pregnant, not pregnant, with embryonic mortality. pregnancy was diagnosed by ultrasonography and plasma concentrations of pregnancy-associated glycoproteins (pags). our results showed that, ... | 2016 | 26850546 |
| rapid detection of water buffalo ricotta adulteration or contamination by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry. | the improvement and development of novel technologies and analytical tools are an important first line of defence for both detecting and deterring food frauds. in order to protect the authenticity of traditional foods and safeguard geographical indications and traditional specialities at european level, protected designation of origin (pdo) legislation has been introduced as a guarantee of quality (council regulation ecc 2081/92). dairy products, especially those certified as pdo products, are a ... | 2016 | 26777680 |
| 208 production of transgenic cloned buffalo (bubalus bubalis) embryos containing human insulin gene through hand-guided cloning. | diabetes is a growing disease worldwide and has emerged as a major healthcare problem in india. insulin is an essential medicine for the treatment of diabetes. large dairy animals, such as buffaloes and cows, may be used as bioreactors for cost-effective production of human insulin. the present study was aimed to produce transgenic buffalo embryos containing the human insulin gene through hand-guided cloning for production of transgenic animals. buffalo female fetal fibroblast cells at passage n ... | 2016 | 28279005 |
| 164 carnitine improves post-thawing sperm motility by increasing adenosine triphosphate content in buffalo (bubalus bubalis). | semen cryopreservation plays a critical role for a wide application of both ai and in vitro embryo production in buffalo. in this species, spermatozoa are more susceptible to hazards during freezing and thawing than cattle spermatozoa, thus resulting in lower fertilizing potential (andrabi et al. 2008 anim. reprod. sci. 104, 427-433). carnitine is a quaternary ammonium compound with antioxidant capacities, able to reduce the availability of lipids for peroxidation by transporting fatty acids int ... | 2016 | 28278961 |
| 151 effect of ascorbic acid on oxidative stress and its thermoprotectant role on in vitro embryonic development of buffalo (bubalus bubalis) embryos. | the most important factors that lead to stress in farm animals are oxidative and thermal stress, leading to reduced reproductive efficiency. oxidative stress leads to an increase in proportion of reactive oxygen species, whereas heat stress affects the physiology of animals, which lowers the conception rates of dairy cattle. in vitro culture systems have been enhanced by manipulating media with various supplements such as vitamins, growth factors, and antioxidants that have overcome these proble ... | 2016 | 28278948 |
| 33 buffalo (bubalus bubalis) embryos produced by hand-made cloning and in vitro fertilization differ in their global transcriptome profile. | although the blastocyst rate obtained with nuclear transferred (nt) embryos is higher than that obtained following in vitro fertilization (ivf) in buffalo, the live birth rate of nt embryos is <2% across different farm animal species compared with a birth rate >40% obtained with ivf embryos. this is believed to be due primarily to incomplete or incorrect nuclear reprogramming of the donor somatic cell by the oocyte, which results in aberrant embryonic gene expression. we compared the global tran ... | 2016 | 28278830 |
| 32 next-generation sequencing discloses differences in microrna expression profiles of buffalo (bubalus bubalis) embryos produced by hand-made cloning and in vitro fertilization. | mammalian embryo development is a complex process with a series of critical events taking place at every stage of development. it is an established fact that the birth rate of animals produced by nuclear transfer (nt) is far less (<2%) than that of ivf embryos (40%) after successful embryo transfers in different farm animal species. micro(mi)rnas are small non-coding rnas of 17 to 25 nucleotides that alter the function of their target genes by either degrading them or inhibiting their expression ... | 2016 | 28278829 |
| strong and stable geographic differentiation of swamp buffalo maternal and paternal lineages indicates domestication in the china/indochina border region. | the swamp type of the asian water buffalo is assumed to have been domesticated by about 4000 years bp, following the introduction of rice cultivation. previous localizations of the domestication site were based on mitochondrial dna (mtdna) variation within china, accounting only for the maternal lineage. we carried out a comprehensive sampling of china, taiwan, vietnam, laos, thailand, nepal and bangladesh and sequenced the mtdna cytochrome b gene and control region and the y-chromosomal zfy, sr ... | 2016 | 26677084 |
| mass spectrometry detection of fraudulent use of cow whey in water buffalo, sheep, or goat italian ricotta cheese. | ricotta cheese is a typical italian product, made with whey from various species, including cow, buffalo, sheep, and goat. ricotta cheese nominally manufactured from the last three species may be fraudulently produced using the comparatively cheaper cow whey. exposing such food frauds requires a reliable analytical method. despite the extensive similarities shared by whey proteins of the four species, a mass spectrometry-based analytical method was developed that exploits three species-specific ... | 2016 | 26675863 |
| molecular characterization of buffalo haptoglobin: sequence based structural comparison indicates convergent evolution between ruminants and human. | haptoglobin (hp) protein has high affinity for hemoglobin (hb) binding during intravascular hemolysis and scavenges the hemoglobin induced free radicals. earlier reports indicate about uniqueness of hp molecule in human and cattle, but in other animals, it is not much studied. in this paper, we characterized buffalo hp molecule and determined its molecular structure, evolutionary importance, and tissue expression. comparative analysis and predicted domain structure indicated that the buffalo hp ... | 2016 | 26646629 |
| identification of functional consequence of a novel selection signature in cyp11b1 gene for milk fat content in bubalus bubalis. | genomic selection for traits of economic importance is an emerging approach carrying tremendous potentials. many of polygenic traits as milk fat, protein and yield have been characterize at genomic level and important selection signatures have been identified. cytochrome p450 enzymes are potential loci for affecting many of dairy capabilities. present study was conducted for genomic dissection of cyp11b1 gene in riverine buffaloes and seven genetic variations were identified. out of these, one n ... | 2015 | 26629413 |
| magnetic resonance imaging and computer tomography of brain lesions in water buffaloes and cattle stunned with handguns or captive bolts. | owing to the demand for genuine mozzarella, some 330 water buffaloes are being slaughtered every year in switzerland albeit a stunning procedure meeting animal welfare and occupational safety requirements remains to be established. to provide a basis for improvements, we sized anatomical specifics in water buffaloes and cattle and we assessed brain lesions after stunning with captive bolts or handguns by diagnostic imaging. in water buffaloes and cattle, the median distance from the frontal skin ... | 2016 | 26610289 |
| loss of heat shock protein 70 from apical region of buffalo (bubalus bubalis) sperm head after freezing and thawing. | the post-thaw fertility of frozen-thawed mammalian spermatozoa is substantially low as compared with that of fresh sperm. furthermore, the post-thaw fertility of the cryopreserved buffalo sperm has been reported to be poor as compared with that of cattle sperm. recently, heat shock protein 70 (hsp70) has been found to play a critical role in mammalian fertilization and early embryonic development in boar and cattle. however, the presence of such fertility-related hsp70 in buffalo sperm and its s ... | 2016 | 26607876 |
| profile of muscle tissue gene expression specific to water buffalo: comparison with domestic cattle by genome array. | in contrast with the past, the water buffalo is now not only a draft animal, but also an important food source of milk and meat. it is increasingly apparent that the water buffalo have huge potential for meat production, but its breeding needs to be investigated. regarding the molecular mechanisms involved in the meat quality difference between the buffalo (bubalus bulabis) and yellow cattle (bos taurus), 12 chemical-physical characteristics related to the meat quality of longissimus thoracis mu ... | 2016 | 26598327 |
| a framework radiation hybrid map of buffalo chromosome 1 ordering scaffolds from buffalo genome sequence assembly. | river buffalo chromosome 1 (bbu1) is a sub-metacentric chromosome homologous to bovine chromosomes 1 and 27. in this study, we constructed a new framework radiation hybrid (rh) map from bbu1 using bburh5000 panel adding nine new genes (adrb3, atp2c1, copb2, crygs, p2ry1, slc5a3, slc20a2, sst, and zdhhc2) and one microsatellite (cssm043) to the set of markers previously mapped on bbu1. the new framework rh map of bbu1 contained 141 markers (55 genes, 2 ests, 10 microsatellites, and 74 snps) distr ... | 2015 | 26535622 |
| prospecting major genes in dairy buffaloes. | asian buffaloes (bubalus bubalis) have an important socio-economic role. the majority of the population is situated in developing countries. due to the scarce resources in these countries, very few species-specific biotechnology tools exist and a lot of cattle-derived technologies are applied to buffaloes. however, the application of cattle genomic tools to buffaloes is not straightforward and, as results suggested, despite genome sequences similarity the genetic polymorphisms are different. | 2015 | 26510479 |
| differentiated evolutionary relationships among chordates from comparative alignments of multiple sequences of myod and myog myogenic regulatory factors. | myod and myog are transcription factors that have essential roles in myogenic lineage determination and muscle differentiation. the purpose of this study was to compare multiple amino acid sequences of myogenic regulatory proteins to infer evolutionary relationships among chordates. protein sequences from mus musculus (p10085 and p12979), human homo sapiens (p15172 and p15173), bovine bos taurus (q7ys82 and q7ys81), wild pig sus scrofa (p49811 and p49812), quail coturnix coturnix (p21572 and p34 ... | 2015 | 26505406 |
| in the elephant's seed shadow: the prospects of domestic bovids as replacement dispersers of three tropical asian trees. | as populations of the world's largest animal species decline, it is unclear how ecosystems will react to their local extirpation. due to the unique ecological characteristics of megaherbivores such as elephants, seed dispersal is one ecosystem process that may be affected as populations of large animals are decimated. in typically disturbed south asian ecosystems, domestic bovids (cattle, bosprimigenius, and buffalo, bubalus bubalis) may often be the species most available to replace asian eleph ... | 2015 | 26405735 |
| genetic diversity analysis of buffalo fatty acid synthase (fasn) gene and its differential expression among bovines. | fatty acid synthase (fasn) gene seems to be structurally and functionally different in bovines in view of their distinctive fatty acid synthesis process. structural variation and differential expression of fasn gene is reported in buffalo (bubalus bubalis), a bovine species close to cattle, in this study. amino acid sequence and phylogenetic analysis of functionally important thioesterase (te) domain of fasn revealed its conserved nature across mammals. amino acid residues at te domain, responsi ... | 2016 | 26376068 |
| volume calculation of the cattle (bos taurus l.) and the water buffalo (bos bubalis l.) metapodia with stereologic method. | in this study, stereological volume estimations using 26 cattle metapodia (26 metacarpal and 26 metatarsal bones) and 8 water buffalo metapodia (8 metacarpal and 8 metatarsal bones) were made. for this purpose metapodia were parallel sectioned at 1 cm intervals according to cavalieri principle. grids with 0.4 cm probe intervals were superimposed on top of these sections and the matching points were counted. all of the bone structures and medullar cavity volumes were calculated with the data obta ... | 2015 | 26339814 |
| modification of the kjeldahl noncasein nitrogen method to include bovine milk concentrates and milks from other species. | the objective of our research was to modify the current indirect casein method for bovine milk to enable it to be applied to bovine milk, bovine milk concentrates, and milks of other species that contain a protein concentration up to 9% (wt/wt). our work used a series of bovine milk concentrates from about 3 to 9% protein with the same casein as a percentage of true protein to determine the amount of buffer required and ph of the noncasein nitrogen (ncn) filtrate to achieve consistent estimates ... | 2015 | 26298747 |
| association analysis of polymorphism in thyroglobulin gene promoter with milk production traits in riverine buffalo (bubalus bubalis). | polymorphism within the promoter region of bovine thyroglobulin has been reported to be associated with milk and meat quality. in this study, we investigated the genetic variation within thyroglobulin promoter region of swamp and riverine buffaloes using pcr-sscp technique and sequencing, and also analyzing association of polymorphism with the milk production traits. the study revealed four conformational patterns, a, b, c, and d among 323 buffaloes of two riverine breeds and different swamp pop ... | 2015 | 26273563 |
| controlled breeding and reproductive management in water buffaloes (bubalus bubalis) using eazi breed controlled internal drug release. | buffalo reproduction is considerably affected by late maturity, poor oestrus symptoms and long postpartum periods. this study was undertaken to evaluate the efficiency of eazi breed controlled internal drug release (cidr), an intravaginal progesterone-releasing device, in relation to oestrus and fertility. five hundred true anoestrus buffalo cows, in the age group 4-6 years in 10 villages of dharwad district in karnataka state in india, were randomly selected and treated with cidr for 9 days. tw ... | 2015 | 26244580 |
| first report of natural bohv-1 infection in water buffalo. | | 2015 | 26198212 |
| development, characterization, and pluripotency analysis of buffalo (bubalus bubalis) embryonic stem cell lines derived from in vitro-fertilized, hand-guided cloned, and parthenogenetic embryos. | we present the derivation, characterization, and pluripotency analysis of three buffalo embryonic stem cell (buesc) lines, from in vitro-fertilized, somatic cell nuclear-transferred, and parthenogenetic blastocysts. these cell lines were developed for later differentiation into germ lineage cells and elucidation of the signaling pathways involved. the cell lines were established from inner cell masses (icms) that were isolated manually from the in vitro-produced blastocysts. most of the icms (45 ... | 2015 | 26168169 |
| molecular identification and seasonal infections of species of fasciola in ruminants from two provinces in china. | we determined the prevalence and seasonality of infections by fasciola of goats and bovine species (cattle and water buffalo) in hubei and anhui provinces of china. faecal samples were collected at 2- to 3-month intervals from 200 goats in hubei province and from 152 bovine species in anhui province. all faecal samples were examined for the presence of parasites. we determined the nucleotide sequences of the first and second internal transcribed spacers (its-1 and its-2) of the nuclear ribosomal ... | 2016 | 26123576 |
| maldi-tof-ms platform for integrated proteomic and peptidomic profiling of milk samples allows rapid detection of food adulterations. | adulteration of ovine, caprine, and buffalo milks with more common bovine material occurs for economic reasons and seasonal availability. frauds are also associated with the use of powdered milk instead of declared, fresh material. in this context, various analytical methods have been adapted to dairy science applications with the aim to evaluate adulteration of milk samples, although time-consuming, suitable only for speciation or thermal treatment analysis, or useful for a specific fraud type. ... | 2015 | 26098723 |
| controlled breeding and reproductive management in water buffaloes (<i>bubalus bubalis</i>) using eazi breed controlled internal drug release. | buffalo reproduction is considerably affected by late maturity, poor oestrus symptoms and long postpartum periods. this study was undertaken to evaluate the efficiency of eazi breed controlled internal drug release (cidr), an intravaginal progesterone-releasing device, in relation to oestrus and fertility. five hundred true anoestrus buffalo cows, in the age group 4-6 years in 10 villages of dharwad district in karnataka state in india, were randomly selected and treated with cidr for 9 days. tw ... | 2015 | 28235292 |
| cholesterol and fatty acid composition of longissimus thoracis from water buffalo (bubalus bubalis) and brahman-influenced cattle raised under savannah conditions. | male (n=66) water buffalo (buffalo) and brahman-influenced cattle (brahman) were born, raised, weaned, fattened on grazing savannah and harvested at two different ages (19 and 24months) to compare lipid composition of the longissimus thoracis muscle. half of the animals were castrated at seven months of age (moa) to examine the castration effects. at 24 moa brahman steers showed the highest content of total lipids (p<0.05). no significant variation was detected in cholesterol content for either ... | 2015 | 25879797 |
| identification, molecular characterization, and tissue expression of parathyroid hormone-related protein gene (pthrp) from water buffalo (bubalus bubalis). | parathyroid hormone-related protein (pthrp) is involved in the deposition of milk calcium in mammal lactation, but its role in buffalo is unclear. in this study, the full-length coding sequence of the water buffalo pthrp gene was first isolated using reverse transcription-polymerase chain reaction. the protein was then subjected to molecular characterization using bioinformatic methods, and the tissue expression pattern was further assayed by semi-quantitative reverse-transcription polymerase ch ... | 2015 | 25867375 |
| seasonal variations of the ovarian activity and pregnancy rate in the egyptian buffalo cows (bubalus bubalis). | the objective of this study was to investigate the effect of season on the follicular and luteal dynamics and pregnancy rate in egyptian buffaloes. a total of 327 genital tracts and 596 animals were used. the genital tracts were examined in winter (n = 58), spring (n = 179), summer (n = 49), and autumn (n = 41) for follicular population, incidence of presence of developed or mature corpus luteum (cl), and diameters of the preovulatory follicle (of) and the mature cl. buffaloes were mated in wint ... | 2015 | 25778728 |
| short communication: variable number of tandem repeat polymorphisms in dgat1 gene of buffaloes (bubalus bubalis) is associated with milk constituents. | the diacylglycerol-o-transferase 1 gene is a positional and functional candidate for milk composition traits. the objective of this study was to evaluate the segregation of the variable number of tandem repeat polymorphisms in the regulatory region of diacylglycerol-o-transferase 1 gene in a water buffalo herd, and to assess the association of this mutation with milk production traits. for this purpose, 196 murrah buffalo cows were genotyped by pcr. the association of the marker with total milk, ... | 2015 | 25726116 |
| molecular epidemiology and phylogenetic analysis of diverse bovine astroviruses associated with diarrhea in cattle and water buffalo calves in china. | astroviruses are the principal causative agents of gastroenteritis in humans and have been associated with diarrhea in other mammals as well as birds. however, astroviral infection of animals had been poorly studied. in the present study, 211 rectal swabs collected from cattle and water buffalo calves with mild to severe diarrhea were tested for bovine astrovirus (bastv) by rt-pcr. results: 92/211 (43.6%) samples were positive for bastv, at a rate of 46.10% (71/154) in cattle and 36.84% (21/57) ... | 2015 | 25716289 |
| comparative expression analysis of gametogenesis-associated genes in foetal and adult bubaline (bubalus bubalis) ovaries and testes. | this study was conducted to identify and analyse the expression of gametogenesis-associated genes and proteins in foetal and adult buffalo gonads of both the sexes. relative quantification of the genes was determined by qpcr and western blotting. immunohistochemistry was also performed for various gametogenesis-associated proteins in foetal and adult gonads of both the sexes. we observed significantly (p < 0.05) increased expression of primordial germ cell-specific, meiotic as well as genes asso ... | 2015 | 25703697 |
| dige based proteome analysis of mammary gland tissue in water buffalo (bubalus bubalis): lactating vis-a-vis heifer. | mammary gland is an exocrine and sebaceous gland made up of branching network of ducts that end in alveoli. milk is synthesized in the alveoli and secreted into alveolar lumen. mammary gland represents an ideal system for the study of organogenesis that undergoes successive cycles of pregnancy, lactation and involution. to gain insights on the molecular events that take place in pubertal and lactating mammary gland, we have identified 43 differentially expressed proteins in mammary tissue of hei ... | 2015 | 25661041 |
| a review of recent developments in buffalo reproduction - a review. | the buffalo is an important livestock resource in several countries of south asia and the mediterranean regions. however, reproductive efficiency is compromised due to known problems of biological and management origins, such as lack of animal selection and poor nutrition. under optimal conditions puberty is attained at 15 to 18 months in river buffalo, 21 to 24 months in swamp buffalo and is influenced by genotype, nutrition, management and climate. however, under field conditions these values ... | 2015 | 25656203 |
| effects of fetal bovine serum and estrus buffalo serum on maturation of buffalo (bubalus bubalis) oocytes in vitro. | the aim was to assess the effects of fetal bovine serum (fbs) and estrus buffalo serum (ebs) on in vitro maturation rate of oocytes in buffalo. | 2015 | 27047063 |
| production technology and characterization of fior di latte cheeses made from sheep and goat milks. | innovation in the small ruminant dairy sector faces structural challenges because dairies are often involved in breeding and produce cheeses that appeal essentially to local markets using traditional technologies and facilities. an investigation was carried out to produce fior di latte, a traditional, soft pasta filata cheese, from sheep and goat milks at the farm level. fior di latte is an italian high-moisture, round mozzarella currently produced from cow and water buffalo milks; it is very po ... | 2015 | 25547300 |
| differential histone modification status of spermatozoa in relation to fertility of buffalo bulls. | in this study genome-wide di-methylated h3k4 (h3k4me2) and tri-methylated h3k27 (h3k27me3) modification profiles were analyzed in spermatozoa of buffalo bulls having wide fertility differences. the custom designed 4 × 180 k buffalo (bubalus bubalis) chip-on-chip array was fabricated by employing array-based sequential hybridization using bovine and buffalo genomic dna for comparative hybridization. the buffalo specific array developed had 177,440 features assembled from coding sequences, promote ... | 2015 | 25501625 |
| comparative analysis of the river buffalo somatostatin gene. | the somatostatin protein plays a crucial role in the regulation of multiple biological functions, such as growth, fat deposition, and nutrient absorption in vertebrates. polymorphisms in the somatostatin gene have been associated with growth traits in livestock species, including cattle and goat. in this study, we conducted complete molecular characterization of the somatostatin gene in bubalus bubalis (murrah breed) by sequencing a murrah bac clone spanning 72,489 base pairs (bp) in length. the ... | 2014 | 25501213 |
| comparative efficacies of six different media for cryopreservation of immature buffalo (bubalus bubalis) calf testis. | buffalo calves have a high mortality rate (~80%) in commercial dairies and testis cryopreservation can provide a feasible option for the preservation of germplasm from immature males that die before attaining sexual maturity. the aim of the present study was to evaluate combinations of 10 or 20% dimethylsulfoxide (dmso) with 0, 20 or 80% fetal bovine serum (fbs) for cryopreservation of immature buffalo testicular tissues, subjected to uncontrolled slow freezing. tissues cryopreserved in 20% dmso ... | 2014 | 25482277 |
| temporal leakage of cu,zn superoxide dismutase and loss of two low-molecular-weight forms of glutathione peroxidase-1 from buffalo (bubalus bubalis) sperm after freezing and thawing. | the postthaw motility and fertility of frozen-thawed buffalo spermatozoa are substantially low as compared with those of cattle sperm. the sperm motility and fertility have been positively correlated with the antioxidant enzyme activities of human and canine sperm. however, the extent of antioxidant enzyme loss during cryopreservation, although reported for human and cattle sperm, is still not clear for buffalo sperm. thus, in the present study, an attempt was made to determine the activities of ... | 2015 | 25459023 |
| detection of fraudulent addition of bovine whey in water buffalo ricotta cheese by isoelectric focusing. | prevention of food fraud in the dairy field is a difficult issue for researchers, industries and policy makers, both for commercial and health reasons. currently, no analytical method allows detection of the addition of bovine whey to water buffalo ricotta, so this fraudulent practice cannot be prevented. the authors' aim was to develop such a method. | 2015 | 25425399 |
| molecular cloning and expression analysis of the stat1 gene in the water buffalo (bubalus bubalis). | signal transducer and activator of transcription 1 (stat1) is a critical component of the transcription factor complex in the interferon (ifn) signaling pathways. of the seven stat isoforms, stat1 is a key mediator of type i and type iii ifn signaling, but limited information is available for the stat genes in the water buffalo. here, we amplified and identified the complete coding sequence (cds) of the buffalo stat1 gene by using reverse transcription polymerase chain reaction (rt-pcr). sequenc ... | 2015 | 25336386 |
| a comparative protein profile of mammalian erythrocyte membranes identified by mass spectrometry. | a comparative analysis of erythrocyte membrane proteins of economically important animals, goat (capra aegagrus hircus), buffalo (bubalus bubalis), pig (sus scrofa), cow (bos tauras), and human (homo sapiens) was performed. solubilized erythrocyte membrane proteins were separated by sodium dodecyl sulfate-polyacryamide gel electrophoresis (sds-page), visualized by staining the gels with commassie brilliant blue (cbb), and identified by matrix assisted laser desorption ionization time-of-flight m ... | 2014 | 25150706 |
| comparison of endocrine changes, timing of ovulations, ovarian follicular growth, and efficacy associated with estradoublesynch and heatsynch protocols in murrah buffalo cows (bubalus bubalis). | poor estrus expression and the difficulty encountered in predicting the time of ovulation compromise the reproductive efficiency of murrah buffalo cows. synchronization of ovulation and timed artificial insemination are able to precisely control the time of ovulation and thus avoid the need for estrus detection. recently, the estradoublesynch protocol (administration of a pgf2α injection 2 days before heatsynch protocol; gnrh 0, pgf2α 7, estradiol benzoate [eb] 8) was developed that precisely sy ... | 2014 | 25145622 |
| molecular characterization of oxytocin receptor gene in water buffalo (bubalus bubalis). | buffaloes are known for their productivity as compared to average yielding cows due to higher fat percentage, better feed conversion ability and disease resistance. on the other hand, the reproductive performances of buffaloes are often considered as poor owing to late sexual maturity, weak/silent oestrus, repeat breeder and prolonged intercalving interval. the study of cascade of events during oestrus and oestrous cycle can be useful for the improvement of reproductive efficiency of buffaloes. ... | 2014 | 25132183 |
| karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed. | domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. in order to have a better characterization of these different cytotypes of buffaloes, and considering that nor-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (ag-nor) and perfo ... | 2014 | 25071402 |
| effect of cattle breeds on milk composition and technological characteristics in china. | cattle breeds have a striking effect on milk, including milk composition and technological characteristics. this study aims to compare milk composition, acidification activity, viscosity, milk dispersion system stability and casein molecular weight among three buffalo breeds in china. the technological characteristics of milk produced by three cattle breeds of river buffalo (murrah), crossbreed 1st generation (f1), crossbreed multiple generation (fh, h≥3) buffaloes were investigated. cattle bree ... | 2013 | 25049865 |
| comparison of α1-antitrypsin, α1-acid glycoprotein, fibrinogen and nox as indicator of subclinical mastitis in riverine buffalo (bubalus bubalis). | mastitis set apart as clinical and sub clinical is a disease complex of dairy cattle, with sub clinical being the most important economically. of late, laboratories showed interest in developing biochemical markers to diagnose sub clinical mastitis (scm) in herds. many workers reported noteworthy alternation of acute phase proteins (apps) and nitric oxide, (measured as nitrate+nitrite = nox) in milk due to intra-mammary inflammation. but, the literature on validation of these parameters as indic ... | 2013 | 25049851 |
| water buffalo genome characterization by the illumina bovinehd beadchip. | to define the best strategies for genomic association studies and genomic selection, it is necessary to determine the extent of linkage disequilibrium (ld) and the genetic structure of the study population. the current study evaluated the transference of genomic information contained in the illumina bovinehd beadchip from cattle to buffaloes, and assessed the extent of the ld in buffaloes. of the 688,593 bovine single nucleotide polymorphism (snp) that were successfully genotyped from the 384 bu ... | 2014 | 25036164 |
| detection of sex chromosome aneuploidy in dog spermatozoa by triple color fluorescence in situ hybridization. | with the development of a direct visualization of sex chromosome in a single sperm by fluorescence in situ hybridization (fish) technique, the frequency of aberration (aneuploidy) in spermatozoa in several mammals has been investigated. however, there is no report in the incidence of x-y aneuploidy in the sperm population of dogs. therefore, in this study, the aneuploidy in dog spermatozoa was examined by multicolor fish using specific molecular probes for canine sex chromosomes and autosome. se ... | 2014 | 24962971 |
| serum biochemical and haematological reference intervals for water buffalo bubalus bubalis heifers. | based on a review of the literature, reference intervals for water buffalo (bubalus bubalis) serum biochemistry and haematology have not previously been published. the current study was done to establish reference intervals for water buffalo heifers. the international federation of clinical chemistry stated that at least 120 values are necessary to obtain reliable estimates for reference intervals. a total number of 127 clinically healthy buffalo heifers (1-2 years old) were included in the stud ... | 2014 | 24831856 |
| identification of three novel myeloid cathelicidin cdnas and their predicted peptides in buffalo (bubalus bubalis). | antimicrobial peptides (amps) are broad spectrum antibiotics, which mostly act without specific receptors. identification of amps is important in the current scenario of emerging multi-drug resistant bacteria. in the present study, in an attempt to identify new amps, myeloid cathelicidin cdnas were synthesized from buffalo (bubalus bubalis) bone marrow and were amplified using specific primers. sequence analysis of cloned cdnas revealed three novel myeloid cathelicidins. they were named based on ... | 2013 | 24772945 |
| preliminary molecular characterizations of sarcoptes scaibiei (acari: sarcoptidae) from farm animals in egypt. | little is known about the genetic diversity of sarcoptes scabiei mites in farm animals in egypt. in this study, we characterized s. scabiei in 25 skin scrapes from water buffalo, cattle, sheep, and rabbits at the nuclear marker its2 and mitochondrial markers cox1 and 16s rrna. sequences of the its2 showed no host segregation or geographical isolation, whereas those of the mitochondrial cox1 and 16s rrna genes indicated the presence of both host-adapted and geographically segregated populations o ... | 2014 | 24728386 |
| effect of leptin on in vitro nuclear maturation and apoptosis of buffalo (bubalus bubalis) oocyte. | leptin, as a 16 kda adipokine, is a pleiotropic cytokine-like hormone that primarily secreted from adipose tissue. it also involves in the regulation of energy homeostasis, neuroendocrine function, immunity, lipid and glucose homeostasis, fatty acid oxidation, angiogenesis, puberty and reproduction. the aim of this study was to investigate the effects of in vitro addition of leptin to in vitro maturation (ivm) medium on buffalo oocyte maturation and apoptosis. | 2014 | 24696768 |
| bovine calves as ideal bio-indicators for fluoridated drinking water and endemic osteo-dental fluorosis. | relative susceptibility to fluoride (f) toxicosis in the form of osteo-dental fluorosis was observed in an observational survey of 2,747 mature and 887 immature domestic animals of diverse species living in areas with naturally fluoridated (>1.5 ppm f) drinking water. these animals included buffaloes (bubalus bubalis), cattle (bos taurus), camels (camelus dromedarius), donkeys (equus asinus), horses (equus caballus), goats (capra hircus), and sheep (ovis aries). of these mature and immature anim ... | 2014 | 24671615 |
| development of a sequential multicolor-fish approach with 13 chromosome-specific painting probes for the rapid identification of river buffalo (bubalus bubalis, 2n = 50) chromosomes. | the development of new molecular techniques (array cgh, m-fish, sky-fish, etc.) has led to great advancements in the entire field of molecular cytogenetics. however, the application of these methods is still very limited in farm animals. in the present study, we report, for the first time, the production of 13 river buffalo (bubalus bubalis, 2n = 50) chromosome-specific painting probes, generated via chromosome microdissection and the dop-pcr procedure. a sequential multicolor-fish approach is a ... | 2014 | 24664789 |
| serum biochemical and haematological reference intervals for water buffalo (<i>bubalus bubalis</i>) heifers. | based on a review of the literature, reference intervals for water buffalo (bubalus bubalis) serum biochemistry and haematology have not previously been published. the current study was done to establish reference intervals for water buffalo heifers. the international federation of clinical chemistry stated that at least 120 values are necessary to obtain reliable estimates for reference intervals. a total number of 127 clinically healthy buffalo heifers (1-2 years old) were included in the stud ... | 2014 | 28235298 |
| comparative fish mapping of bmpr1b, bmp15 and gdf9 fecundity genes on cattle, river buffalo, sheep and goat chromosomes. | | 2013 | 24371182 |
| cryogenic changes in proteases and antiprotease activities of buffalo (bubalus bubalis) and cattle (bos taurus) semen. | the postthaw motility and fertility of buffalo and cattle semen is reduced when they are cryopreserved for artificial insemination. in the present study, an attempt was made to characterize the cryogenic changes in proteases and antiprotease activities (apa) of buffalo and cattle semen because these proteolysis regulators have been reported to be associated with sperm motility and fertility. buffalo sperm demonstrated at least two major proteases of 45 and 42 kda and three minor proteases of 95, ... | 2014 | 24210916 |
| molecular cloning, sequence characterization, and gene expression profiling of a novel water buffalo (bubalus bubalis) gene, agpat6. | several 1-acylglycerol-3-phosphate-o-acyltransferases (agpats) can acylate lysophosphatidic acid to produce phosphatidic acid. of the eight agpat isoforms, agpat6 is a crucial enzyme for glycerolipids and triacylglycerol biosynthesis in some mammalian tissues. we amplified and identified the complete coding sequence (cds) of the water buffalo agpat6 gene by using the reverse transcription-polymerase chain reaction, based on the conversed sequence information of the cattle or expressed sequence t ... | 2013 | 24114207 |
| the mammary gland in domestic ruminants: a systems biology perspective. | milk and dairy products are central elements in the human diet. it is estimated that 108kg of milk per year are consumed per person worldwide. therefore, dairy production represents a relevant fraction of the economies of many countries, being cattle, sheep, goat, water buffalo, and other ruminants the main species used worldwide. an adequate management of dairy farming cannot be achieved without the knowledge on the biological mechanisms behind lactation in ruminants. thus, understanding the mo ... | 2013 | 24076120 |
| cellular and functional characterization of buffalo (bubalus bubalis) corpus luteum during the estrous cycle and pregnancy. | in the present paper, cellular composition of buffalo corpus luteum (cl) with its functional characterization based on 3β-hsd and progesterone secretory ability at different stages of estrous cycle and pregnancy was studied. buffalo uteri along with ovaries bearing cl were collected from the local slaughter house. these were classified into different stages of estrous cycle (stage i, ii, iii and iv) and pregnancy (stage i, ii and iii) based on morphological appearance of cl, surface follicles on ... | 2013 | 23896394 |
| gas chromatographic-mass spectrometric analysis of chemical volatiles in buffalo (bubalus bubalis) urine. | isolation of active fraction and characterization of chemosignals from urine have been attempted in several mammalian species in the recent years. the objective of this study was to identify the urinary volatiles across various reproductive stages of buffalo cow, namely, estrus, diestrus, and pregnancy, and in bull, by chemical extraction followed by gas chromatography-linked mass spectrometry (gc-ms). urine samples were collected from six buffalo cows at two different phases of estrous cycle, n ... | 2013 | 23876684 |
| homology modeling and structural comparison of leucine rich repeats of toll like receptors 1-10 of ruminants. | toll-like receptors (tlrs) are transmembrane receptors composed of extra cellular leucine rich repeats (lrrs) that identify specific pathogen associated molecular patterns triggering a innate immune cascade. the lrr regions of tlr 1-10 proteins of goat (capra hircus), sheep (ovis aries), buffalo (bubalus bubalis) and bovine (bos taurus) were modeled using modeller 9v7 tool and validated. the similarities and variations of these 10 tlrs extracellular regions of each species were compared using on ... | 2013 | 23812948 |
| the behaviour and welfare of buffaloes (bubalus bubalis) in modern dairy enterprises. | this review deals with the behaviour of river buffaloes (bubalus bubalis), in confinement and in extensive conditions, also focusing on the effects of different housing and rearing conditions on their welfare. the behavioural repertoire expressed by buffaloes in extensive and intensive conditions is similar to those displayed by other domestic ruminants. however, through natural selection, buffaloes have also acquired several morphological, physiological and behavioural (i.e. wallowing) adaptati ... | 2013 | 23803231 |
| molecular characterization and expression profile of ghrelin gene during different reproductive phases in buffalo (bubalus bubalis). | ghrelin, a novel motilin-related endogenous ligand for growth hormone secretagouge receptor, is implicated in various biological functions, including regulation of female reproduction. but the presence of ghrelin and its role in reproductive functions in buffalo, a species with poor reproductive efficiency, is not known. in the present study full-length ghrelin cdna was isolated from bubaline abomasum, which encodes the entire prepropeptide of 116 amino acids. the deduced amino acid sequence of ... | 2013 | 23796362 |
| importance of the horse and financial impact of equine trypanosomiasis on cattle raising in venezuela. | in venezuela, horses are indispensable for extensive cattle raising, and extensive cattle raising prevails in all regions. this determines the numerical relationship between horses and cattle (r = 0.93) to be relatively constant nationwide. at regional level, the average extension of cattle ranches varies greatly. however, in relation to the area covered by pastures, the numbers of horses (r = 0.95) and cattle (r = 0.93) are relatively uniform nationwide. water buffalo occupy small fractions of ... | 2013 | 23666515 |
| effects of a single nucleotide polymorphism in the leptin gene on the productive traits of dairy buffaloes (bubalus bubalis). | the gene responsible for coding the leptin hormone has been associated with productive and reproductive traits in cattle. in dairy cattle, different polymorphisms found in the leptin gene have been associated with several traits of economic interest, such as energy balance, milk yield and composition, live weight, fertility and dry matter consumption. the aim of this study was to detect genetic variability in the leptin gene of buffaloes and to test possible associations with milk yield, fat and ... | 2013 | 23661024 |
| physical mapping of 20 unmapped fragments of the btau_4.0 genome assembly in cattle, sheep and river buffalo. | the recent advances in sequencing technology and bioinformatics have revolutionized genomic research, making the decoding of the genome an easier task. genome sequences are currently available for many species, including cattle, sheep and river buffalo. the available reference genomes are very accurate, and they represent the best possible order of loci at this time. in cattle, despite the great accuracy achieved, a part of the genome has been sequenced but not yet assembled: these genome fragme ... | 2013 | 23652984 |
| antibodies to schmallenberg virus in domestic livestock in turkey. | this, partly retrospective study, was designed to determine the seroprevalence of schmallenberg virus (sbv), a new orthobunyavirus first reported in germany in late 2011, in domestic ruminants from the middle black sea, west, and southeast regions of turkey. an indirect enzyme-linked immunosorbent assay was used to screen serum samples collected from slaughterhouse animals between 2006 and 2013. the overall seroprevalence was 335/1,362 (24.5 %) with 325/816 (39.8 %), 5/307 (1.6 %), 3/109 (2.8 %) ... | 2013 | 23645515 |
| minimum number of spermatozoa per dose in mediterranean italian buffalo (bubalus bubalis) using sexed frozen semen and conventional artificial insemination. | in buffaloes, ai with sexed semen is not fully optimized, and the procedure has only been performed using the approach currently in use for cattle. the objective of the present work was to compare the pregnancy rates in mediterranean italian buffalo cows inseminated with sexed frozen-thawed semen at 2, 4, 6, and 8 million sperm per dose, using the ovsynch protocol and conventional ai at a fixed time. fresh ejaculates from three buffalo bulls were processed according to beltsville sperm sorting t ... | 2013 | 23523175 |
| in silico mining of putative microsatellite markers from whole genome sequence of water buffalo (bubalus bubalis) and development of first buffsatdb. | though india has sequenced water buffalo genome but its draft assembly is based on cattle genome btau 4.0, thus de novo chromosome wise assembly is a major pending issue for global community. the existing radiation hybrid of buffalo and these reported str can be used further in final gap plugging and "finishing" expected in de novo genome assembly. qtl and gene mapping needs mining of putative str from buffalo genome at equal interval on each and every chromosome. such markers have potential rol ... | 2013 | 23336431 |
| investigation of transferability of bovinesnp50 beadchip from cattle to water buffalo for genome wide association study. | cattle and water buffalo belong to the same subfamily bovinae and share chromosome banding and gene order homology. in this study, we used genome-wide illumina bovinesnp50 beadchip to analyze 91 dna samples from three breeds of water buffalo (nili-ravi, murrah and their crossbred with local guangxi buffalos in china), to demonstrate the genetic divergence between cattle and water buffalo through a large single nucleotide polymorphism (snp) transferability study at the whole genome level, and per ... | 2013 | 23232712 |
| a high-resolution radiation hybrid map of the river buffalo major histocompatibility complex and comparison with bola. | the major histocompatibility complex (mhc) in mammals codes for antigen-presenting proteins. for this reason, the mhc is of great importance for immune function and animal health. previous studies revealed this gene-dense and polymorphic region in river buffalo to be on the short arm of chromosome 2, which is homologous to cattle chromosome 23. using cattle-derived sts markers and a river buffalo radiation hybrid (rh) panel (bburh5000 ), we generated a high-resolution rh map of the river buffalo ... | 2013 | 23216319 |
| separation and quantification of water buffalo milk protein fractions and genetic variants by rp-hplc. | a rp-hplc method, developed for the separation and quantification of the most common genetic variants of bovine milk proteins, was successfully applied to the analysis of water buffalo milk. all the most common buffalo casein and whey proteins fractions, as well as their genetic variants, were detected and separated simultaneously in 40 min. purified buffalo proteins were used as calibration standards and a total of 536 individual milk samples were analysed for protein composition. α(s1)-, α(s2) ... | 2013 | 23122071 |
| new method for the simultaneous identification of cow, sheep, goat, and water buffalo in dairy products by analysis of short species-specific mitochondrial dna targets. | a novel method is presented here as an analytical tool for food control and authentication of dairy products manufactured from the milk of cow, sheep, goat, and buffalo. the method is based on multiplex polymerase chain reaction (pcr) of species-specific mitochondrial dna (mtdna) targets followed by fragment size analysis by capillary electrophoresis. the method includes (a) simultaneous detection of four species, (b) internal control for dna extraction and pcr, (c) mtdna as a target for pcr, (d ... | 2012 | 23025240 |
| a single nucleotide polymorphism in the promoter region of river buffalo stearoyl coa desaturase gene (scd) is associated with milk yield. | an association study between the milk yield trait and the stearoyl-coa desaturase (scd) polymorphism (g.133a > c) in italian mediterranean river buffalo was carried out. a full characterization of the river buffalo scd promoter region was presented. genotyping information was provided and a quick method for allelic discrimination was developed. the frequency of the c allele was 0·16. test-day (td) records (43 510) of milk production belonging to 226 lactations of 169 buffalo cows were analysed w ... | 2012 | 22994977 |
| sex-sorting sperm using flow cytometry/cell sorting. | the sex of mammalian offspring can be predetermined by flow sorting relatively pure living populations of x- and y-chromosome-bearing sperm. this method is based on precise staining of the dna of sperm with the nucleic acid-specific fluorophore, hoechst 33342, to differentiate between the subpopulations of x- and y-sperm. the fluorescently stained sperm are then sex-sorted using a specialized high speed sorter, moflo(®) sx xdp, and collected into biologically supportive media prior to reconcentr ... | 2013 | 22992923 |
| male-specific repeats in wild bovidae. | in this study, we investigated repetitive sequences localized on y chromosomes. repetitive dna sequences represent a substantial part of the eukaryotic genome and, among them, a large portion comprises sequences repeated in tandem. efficient and rapid isolation of repeat units is possible due to a laser microdissection technique used for y chromosome separation, followed by polymerase chain reaction (pcr), cloning, and sequence analysis. we applied the derived repeat units to members of nine tri ... | 2012 | 22895838 |
| interferon stimulated gene 15 (isg15): molecular characterization and expression profile in endometrium of buffalo (bubalus bubalis). | interferon stimulated gene 15 (isg15), one of the several proteins induced by conceptus derived type i and/or a type ii interferon (ifn), is implicated as an important factor in determining the uterine receptivity and conceptus development. however, presence as well as specific role of the isg15 in buffalo (bubalus bubalis) reproduction is yet to be elucidated. in the present study, both genomic and cdna sequences of bubaline (bu) isg15 were cloned and investigated for its expression in differen ... | 2012 | 22871329 |
| metagenomic analysis of virulence-associated and antibiotic resistance genes of microbes in rumen of indian buffalo (bubalus bubalis). | a major research goal in rumen microbial ecology is to understand the relationship between community composition and its function, particularly involved in fermentation process is of a potential interest. the buffalo rumen microbiota impacts human food safety as well as animal health. although the bacteria of bovine rumen have been well characterized, techniques have been lacking to correlate total community structure with gene function. we applied 454 next generations sequencing technology to c ... | 2012 | 22850272 |
| isolation and enrichment of type a spermatogonia from pre-pubertal buffalo ( bubalus bubalis) testis. | the aim of this study was to isolate and subsequently enrich type a spermatogonia from pre-pubertal buffalo testis. two-step enzymatic digestion was used to isolate spermatogonia from 10 to 14 months pre-pubertal buffalo calves, resulting in maximal release of spermatogonia from the seminiferous tubules. after enzymatic digestion, the type a spermatogonia were subsequently enriched by differential plating and percoll gradient centrifugation. the identity of type a spermatogonia was determined by ... | 2013 | 22742713 |
| sequence characterization of river buffalo toll-like receptor genes 1-10 reveals distinct relationship with cattle and sheep. | the present study was undertaken to characterize the full-length transcripts of toll-like receptor (tlr) genes 1-10 of river buffalo. the conceptualized amino acid identity of bubaline tlrs ranged between 86% to 100% with ruminants, while it ranged between 45% to 91% with other vertebrate species. simple modular architecture tool (smart) analysis revealed the presence of tir domains and varying numbers of leucine-rich repeat motifs in all the buffalo tlrs. with respect to tir domains, tlrs 1, 2 ... | 2013 | 22694123 |
| advanced comparative cytogenetic analysis of x chromosomes in river buffalo, cattle, sheep, and human. | based on a recently generated comprehensive gene map for ovis aries chromosome x (oarx) with an approximately even locus distribution, we assigned selected bacterial artificial chromosome (bac) probes corresponding to these oarx loci to bubalus bubalis (bbu) and bos taurus (bta) by comparative fluorescence in-situ hybridization (fish) to improve cytogenetically the x chromosome maps in these species. twenty-five added loci in bbux and btax, respectively, contribute to a more detailed description ... | 2012 | 22669522 |
| population structure and genetic variability in the murrah dairy breed of water buffalo in brazil accessed via pedigree analysis. | the objective of this study was to use pedigree analysis to evaluate the population structure and genetic variability in the murrah dairy breed of water buffalo (bubalus bubalis) in brazil. pedigree analysis was performed on 5,061 animals born between 1972 and 2002. the effective number of founders (fe) was 60, representing 6.32 % of the potential number of founders. the effective number of ancestors (fa) was 36 and the genetic contribution of the 17 most influent ancestors explained 50 % of the ... | 2012 | 22535150 |
| influence of intergeneric/interspecies mitochondrial injection; parthenogenetic development of bovine oocytes after injection of mitochondria derived from somatic cells. | interspecies/intergeneric mitochondrial heteroplasmy can occur in interspecies/intergeneric hybrid embryos or following nuclear transfer. in the present study, intergeneric buffalo (bubalus bubalis) mitochondria (wb-mt) or interspecies murine (mus spretus) mitochondria (m-mt) were injected into bovine (bos taurus) oocytes, and the subsequent embryonic development was characterized. fibroblast mitochondria (wb-mt or m-mt) were microinjected into in vitro matured bovine oocytes followed by oocyte ... | 2012 | 22447326 |
| chromosome fragility in river buffalo cows exposed to dioxins. | fifty river buffalo (bubalus bubalis, 2n = 50) cows reared in two different provinces of campania (southern italy) underwent cytogenetic investigations to ascertain possible differences in their chromosome stability. one group (caserta province) was under legal sequestration due to the presence in the milk mass of higher mean values of dioxins [21.79 pg/g of fat as sum of polychloro-dibenzo-dioxins (pcdds), polychloro-dibenzo-furans (pcdfs) and dioxin-like polychlorobiphenyls (dl-pcbs)] than bot ... | 2012 | 22415351 |
| animal production systems of small farms in the kaski district of nepal. | the aim of this study was to describe animal production on small farms in the kaski district of nepal, with the goal of identifying areas where animal health and productivity could be improved. eighty-five randomly selected farms from four different village development committees were visited. farmers were interviewed and premises and animals visually inspected on all farms. feed samples were collected from a subset of farms. the most commonly kept species were water buffalo (used for milk and m ... | 2012 | 22392548 |