| the role of adenosine a(1) receptors in the atp-evoked ca(2+) response in rat thyroid frtl-5 cells. | the effect of adenosine a(1) receptor activation on the atp-induced increase in intracellular free ca(2+) was studied in control and protein kinase c down-regulated fisher rat thyroid (frtl-5) cells. long-term phorbol ester treatment, which leads to protein kinase c down-regulation, enhanced the atp-evoked extracellular ca(2+) influx. the increased ca(2+) influx was antagonized by the adenosine a(1) receptor antagonist 8-cyclopentyl-1, 3-dipropylxanthine (dpcpx). [3h]dpcpx binding studies reveal ... | 2000 | 10708705 |
| preliminary assessment of health impacts for the newport chemical agent disposal facility. | a preliminary assessment of health impacts (pahi) study was conducted to look at potential human and environmental health impacts due to the air and water emissions generated from the proposed newport chemical agent disposal facility (necdf) in newport, indiana. as an alternative to incineration, the necdf will use a neutralization-based treatment process followed by supercritical water oxidation to destroy the vx nerve agent stored in ton containers at the newport chemical depot. there is no re ... | 2000 | 10711389 |
| multiple n-acetyl neuraminic acid synthetase (neub) genes in campylobacter jejuni: identification and characterization of the gene involved in sialylation of lipo-oligosaccharide. | n-acetyl neuraminic acid (nana) is a common constituent of campylobacter jejuni lipo-oligosaccharide (los). such structures often mimic human gangliosides and are thought to be involved in the triggering of guillain-barré syndrome (gbs) and miller-fisher syndrome (mfs) following c. jejuni infection. analysis of the c. jejuni nctc 11168 genome sequence identified three putative nana synthetase genes termed neub1, neub2 and neub3. the nana synthetase activity of all three c. jejuni neub gene produ ... | 2000 | 10712693 |
| characterisation of autoantibodies to peripheral myelin protein 22 in patients with hereditary and acquired neuropathies. | to investigate the possibility that an autoimmune mechanism may play a role in the hereditary neuropathy charcot-marie-tooth type 1a (cmt1a), sera were analysed by western blot for anti-peripheral myelin protein 22 (pmp22) autoantibodies. these sera were compared with sera from patients with cmt type 2 (cmt2), acquired peripheral neuropathies such as chronic inflammatory demyelinating neuropathy (cidp), anti-mag igm neuropathy, miller-fisher syndrome (mfs), diabetic neuropathy and with control b ... | 2000 | 10713355 |
| [reduction of acute kidney transplantation rejection by the chemokine receptor antagonist met-rantes]. | chemokines contribute to the mononuclear cell infiltrate in vessels and interstitium which is characteristic of renal transplant rejection. by employing the chemokine receptor blocker met-rantes it was shown that recruitment of inflammatory cells into renal allografts could be significantly suppressed. in a renal transplant model (fisher rt1(1v1) rat kidney into lewis rt1(1) rat) met-rantes-treated animals showed a significant reduction in vascular injury score (16.10 +/- 5.20 vs. 62.67 +/- 18.6 ... | 1999 | 10714211 |
| induction of adaptation to inhaled lipopolysaccharide in young and old rats and mice. | lipopolysaccharide (lps) is a component of the gram-negative bacterial cell wall that is known to activate inflammatory cells and enhance the production of inflammatory mediators in the lung. as it is a ubiquitous compound, inhalation exposure is highly likely in the human environment. adaptation is a phenomenon by which a previous exposure results in improved survival or reduced injury as compared to a single exposure alone. we hypothesized that the basic proinflammatory effects of lps in the l ... | 2000 | 10715626 |
| immunization with a chimeric tobacco mosaic virus containing an epitope of outer membrane protein f of pseudomonas aeruginosa provides protection against challenge with p. aeruginosa. | a chimeric tobacco mosaic virus (tmv) was constructed by inserting sequences representing peptide 9-14mer (tdaynqklserran) of outer membrane (om) protein f of pseudomonas aeruginosa between amino acids ser154 and gly155 of the tmv coat protein (cp). this is the first example of tmv being used to construct a chimera containing a bacterial epitope. mice immunized with tmv-9-14 produced anti-peptide-9-14mer-specific antibodies that reacted in whole-cell elisa with all seven fisher-devlin (fd) immun ... | 2000 | 10717347 |
| quantification of telomerase activity in sporadic colorectal carcinoma: association with tumor growth and venous invasion. | activation of telomerase, a ribonucleoprotein enzyme complex that synthesizes telomere repeats, is associated with acquisition of unlimited cellular proliferation and is commonly detected in human cancer. measurement of telomerase activity (ta) may provide important information as a diagnostic marker or a prognostic indicator. the authors studied the quantification of ta and assessed its utility as a prognostic marker in sporadic colorectal carcinoma. | 2000 | 10717610 |
| age-associated decline in gamma-glutamylcysteine synthetase gene expression in rats. | although glutathione (gsh) concentration has been reported to diminish with age, the mechanism underlying such age-associated decline in the gsh content is not well understood. in this study, we compared the gene expression of both subunits of gamma-glutamylcysteine synthetase (gcs), the rate-limiting enzyme in de novo gsh synthesis, in young, adult, and old fisher 344 rats. it was found that gcs activity was significantly decreased with increased age in liver, kidney, lung, and red blood cells ... | 2000 | 10719238 |
| effect of angiotensin-converting enzyme inhibition on growth factor mrna in chronic renal allograft rejection in the rat. | despite considerable progress in immunosuppression, the incidence of chronic renal allograft rejection has not decreased. recent studies have revealed that angiotensin-converting enzyme (ace) inhibition ameliorates graft arteriosclerosis, glomerulosclerosis, and tubular atrophy. moreover, it decreases systemic and glomerular capillary hydrostatic pressure in a rat kidney allograft model. we evaluated the effects of the ace inhibitor enalapril on cytokine and growth factor expression in chronical ... | 2000 | 10720951 |
| [microsurgical penile revascularization in patients with pure arteriogenic erectile dysfunction]. | we report the results of microscopic penile revascularization in patients with arteriogenic erectile dysfunction. | 2000 | 10723178 |
| maximum effect of urokinase plasminogen activator inhibitors in the control of invasion and metastasis of rat mammary cancer. | experimentally induced pulmonary metastases of mammary cancer in the fisher 344 rat can be suppressed by the inhibition of urokinase plasminogen activator (upa). the inhibition of upa with amiloride or b428 has been shown to be dose dependent. increased dosage levels of inhibitors might be expected to enhance levels of suppression of metastases. the use of each of these inhibitors at equipotent concentrations that exceeded the doses administered in previous studies failed to eliminate pulmonary ... | 1998 | 10729770 |
| neuromuscular dysfunction in the jejunum and colon of human leukocyte antigen b27 transgenic rats. | hla-b27 transgenic rats are a model of spontaneous gastrointestinal inflammation associated with expression of human leukocyte antigen (hla) b27 and beta(2)-microglobulin. our goal was to investigate in vitro enteric nerve regulation and contractile activity in isolated longitudinal muscles from the jejunum and colon of hla-b27 rats. nontransgenic age-matched fisher 344 rats were used as controls. intestinal inflammation and tissue injury, quantified histologically and through tissue myeloperoxi ... | 2000 | 10734153 |
| emphysematous pyelonephritis: clinicoradiological classification, management, prognosis, and pathogenesis. | emphysematous pyelonephritis (epn) is a rare, severe gas-forming infection of renal parenchyma and its surrounding areas. the radiological classification and adequate therapeutic regimen are controversial and the prognostic factors and pathogenesis remain uncertain. | 2000 | 10737279 |
| effects of fetal exposure to nicotine on dental development of the laboratory rat. | nicotine is one of the most widely used toxins in the world today. most addiction research relating to nicotine in particular, as well as opioids and alcohol, has concentrated on the cellular and molecular biology of the mammalian brain and on features of organ structure and physiology associated with substance abuse. thus, while numerous studies have been conducted to examine nicotine's detrimental physiological effects in a variety of soft tissues, this investigation attempts to examine furthe ... | 2000 | 10737858 |
| [probable miller fisher syndrome during dengue fever type 2]. | a 57 year-old-man developed a left ophthalmoplegia associated with ataxia and areflexia while he had fever (39 degrees c) for two days. dengue fever (df) was diagnosed by definite criteria, i.e.: igm seroconversion, positive culture from serum and positive pcr both from serum and csf. within one week, he fully and spontaneously recovered. to our knowledge, this neurological picture has never been reported in df. considering the immune-mediated nature of this condition, its pathogenesis in df is ... | 2000 | 10743016 |
| effect of soft-tissue trauma on the early periosteal response of bone to injury. | to determine whether the periosteal response to skeletal trauma is impaired when muscle is also injured, thereby providing a possible explanation for why fractures with extensive soft-tissue damage may take longer to heal. | 2000 | 10744288 |
| in vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats. | to clarify the effect of hepatocyte growth factor (hgf) on proliferation of hepatic oval cells, we transferred hgf gene into liver of the solt-farber rat model. male fisher 344 rats were infected with a recombinant adenovirus carrying the cdna for hgf (paxcahgf) from tail vein. hgf mrna showed its peak at 4 days, and diminished thereafter. the total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in hgf-transferred rats, in which stem cell factor an ... | 2000 | 10745090 |
| expression analysis of bace2 in brain and peripheral tissues. | beta-site amyloid precursor protein cleaving enzyme (bace) is a novel transmembrane aspartic protease that possesses all the known characteristics of the beta-secretase involved in alzheimer's disease (vassar, r., bennett, b. d., babu-khan, s., kahn, s., mendiaz, e. a., denis, p., teplow, d. b., ross, s., amarante, p., loeloff, r., luo, y., fisher, s., fuller, j., edenson, s., lile, j., jarosinski, m. a., biere, a. l., curran, e., burgess, t., louis, j. -c., collins, f., treanor, j., rogers, g., ... | 2000 | 10749877 |
| inhibition of chronic rejection of aortic allografts by dietary glycine. | chronic rejection is influenced by a variety of risk factors, including histoincompatibility and ischemia. glycine, a cytoprotective agent, has been shown to protect against ischemia-reperfusion injury in the liver, inactivate hepatic resident macrophages, minimize cyclosporin a-induced nephrotoxicity, and exhibit immunosuppressive properties in vitro. the aim of this study was to investigate whether dietary glycine could reduce development of chronic rejection. | 2000 | 10755525 |
| new concepts of guillain-barré syndrome. | guillain-barré syndrome is an acute autoimmune polyradiculoneuropathy with a clinical presentation of flaccid paralysis with areflexia, variable sensory disturbance, and elevated cerebrospinal fluid protein without pleocytosis. although guillain-barré syndrome previously had been viewed as a unitary disorder with variations, it currently is viewed as a group of syndromes with several distinctive subtypes. these include the principal subtype prevalent in the western world (acute inflammatory demy ... | 2000 | 10757475 |
| pathogenesis of abnormal vaginal bacterial flora. | this study was undertaken to determine the relationships between microscopy findings on wet mounts, such as lactobacillary grade or vaginal leukocytosis, and results of vaginal culture, lactate and succinate content of the vagina, and levels of selected cytokines. | 2000 | 10764465 |
| long-bone fractures in llamas: six cases (1993-1998). | to determine clinical and radiographic findings, treatment, and outcome for llamas with long-bone fractures. | 2000 | 10767973 |
| comparison of voxel- and volume-of-interest-based analyses in fdg pet scans of hiv positive and healthy individuals. | abnormal glucose metabolic patterns in the brain have been reported for hiv-1 seropositive (hiv+) patients when compared with seronegative healthy individuals. the metabolic covariance pattern obtained from voxel- or volume-of-interest (voi)-based multivariate data analysis techniques can be used to characterize disease and potentially to detect and monitor disease progression in the early stage of aids dementia complex. however, the arbitrary smoothing typically applied to pet data during recon ... | 2000 | 10768561 |
| strain difference in rats with experimental giant cell myocarditis. | immunogenetic mechanisms may be involved in the pathogenesis of myocarditis and dilated cardiomyopathy. the present study investigated the incidence, histopathology and histocompatibility characteristics of experimental giant cell myocarditis in various strains of rats. experimental giant cell myocarditis was induced by immunization with porcine cardiac myosin in lewis (rt-1(l)), dahl (dir/eis) (rt-1(l)), fisher (rt-1(lv 1)) rats, but not in dahl (dis/eis) (rt-1(l)) or brown norway (rt-1(n)). my ... | 2000 | 10783051 |
| rescue of lesioned adult rat spinal motoneurons by adenoviral gene transfer of glial cell line-derived neurotrophic factor. | glial cell line-derived neurotrophic factor (gdnf) has been shown to protect cranial and spinal motoneurons, that suggests potential uses of gdnf in the treatment of spinal cord injury and motor neuron diseases. we examined neuroprotective effect of human gdnf encoded by an adenovirus vector (axcahgdnf) on the death of lesioned adult rat spinal motoneurons. the seventh cervical segment (c7) ventral and dorsal roots and dorsal root ganglia of adult fisher 344 rats were avulsed, and axcahgdnf, axc ... | 2000 | 10797554 |
| cyclosporine a and azathioprine are equipotent in chronic kidney allograft rejection. | chronic rejection is the major cause of graft loss after kidney transplantation. various immunosuppressive protocols have been used to ameliorate this process. we investigated whether cyclosporin a- (cya) or azathioprine- (aza) based immunosuppression is better able to slow the progression of chronic rejection. fisher kidneys were transplanted into bilaterally nephrectomized lewis rats. recipients received cya (1.5 mg/kg/day, s.c.) for 10 days, and were treated from day 11 with either cya (1.5 m ... | 2000 | 10798743 |
| tolerance induced by bone marrow chimerism prevents transplant vascular sclerosis in a rat model of small bowel transplant chronic rejection. | the major impediment to success in solid organ transplantation is chronic rejection (cr). the characteristic lesion of cr is transplant vascular sclerosis (tvs). although the mechanism of tvs is thought to have an immunologic basis, in humans immunosuppression does not prevent or reverse it. one possible therapy to prevent tvs is induction of donor-specific tolerance. bone marrow chimerism has been successful in inducing tolerance in acute and chronic rejection heart and kidney transplant models ... | 2000 | 10798744 |
| retinal vascular changes induced by the oxidative stress of alpha-tocopherol deficiency contrasted with diabetic microangiopathy. | it has been proposed that oxidative tissue damage is involved in the development of diabetic angiopathies. to evaluate this hypothesis, experiments were conducted to identify the retinal vessel changes induced by the oxidative stress related to alpha-tocopherol deficiency and examine possible similarities with the lesions characteristic of diabetic retinopathy. twenty-one-day-old male fisher 344 albino rats were divided randomly to receive a basal, chemically defined diet either with (adequate g ... | 2000 | 10803422 |
| herpes simplex virus type 2 growth and latency reactivation by cocultivation are inhibited with antisense oligonucleotides complementary to the translation initiation site of the large subunit of ribonucleotide reductase (rr1). | antisense oligonucleotides complementary to the translation initiation site of the herpes simplex virus type 2 (hsv-2) large subunit of ribonucleotide reductase (rr1) were studied for their ability to inhibit rr1 expression, hsv-2 growth, and its reactivation from latently infected ganglia. the oligomers caused a significant decrease (90%-97% inhibition) in hsv-2 rr1 expression and inhibited hsv-2 growth, with ic50 and ic90 values of 0.11 and 1.0 microm, respectively. the titers of hsv-2 mutants ... | 2000 | 10805158 |
| trichloroethylene and cancer: epidemiologic evidence | adopt a traditional review of the mutagenicity data on tce and its metabolites but instead raise several issues regarding the interpretation of mutagenicity and genetic toxicity tests in shedding light on whether these processes are key events in tumor initiation. as discussed in the u.s. epa proposed cancer guidelines, a salient question is whether tce or its metabolites interacts directly with and mutates dna to bring about changes in gene expression or whether dna mutation is achieved through ... | 2000 | 10807549 |
| statistical analysis of fisher et al. pbpk model of trichloroethylene kinetics. | two physiologically based pharmacokinetic models for trichloroethylene (tce) in mice and humans were calibrated with new toxicokinetic data sets. calibration is an important step in model development, essential to a legitimate use of models for research or regulatory purposes. a bayesian statistical framework was used to combine prior information about the model parameters with the data likelihood to yield posterior parameter distributions. for mice, these distributions represent uncertainty. fo ... | 2000 | 10807558 |
| cerebral infarction complicating intravenous immunoglobulin therapy in a patient with miller fisher syndrome. | intravenous immunoglobulin (ivig) therapy is being increasingly used in a wide range of neurological conditions. however, treatment is expensive and side effects may be severe. a patient with miller fisher syndrome who developed cortical blindness as a consequence of occipital infarction precipitated by ivig is reported on. | 2000 | 10811710 |
| quantitative grading of rat esophageal carcinogenesis using computer-assisted image tile analysis. | our objective was to grade, by computer-assisted quantitative image tile analysis, the intraepithelial neoplasia (also called dysplasia) that develops in esophagi of rats given n-nitrosomethybenzylamine (nmba) for 5 weeks. to perform image tile analysis, the computer divides the video image of the neoplastic epithelium into a row of contiguous small rectangular images, or "tiles," 84 x 292 microm in size, and quantitatively measures four selected tissue features within each image tile. the compu ... | 2000 | 10815694 |
| difference in incidence of spontaneous mutations between herpes simplex virus types 1 and 2. | spontaneous mutations within the herpes simplex virus (hsv) genome are introduced by errors during dna replication. indicative of the inherent mutation rate of hsv dna replication, heterogeneous hsv populations containing both acyclovir (acv)-resistant and acv-sensitive viruses occur naturally in both clinical isolates and laboratory stocks. wild-type, laboratory-adapted hsv type 1 (hsv-1) strains kos and cl101 reportedly accumulate spontaneous acv-resistant mutations at a frequency of approxima ... | 2000 | 10817703 |
| genome-wide loss of heterozygosity analysis of chemically induced rat hepatocellular carcinomas reveals elevated frequency of allelic imbalances on chromosomes 1, 6, 8, 11, 15, 17, and 20. | neoplastic development is a multistep process that involves the stochastic accumulation of heritable genetic alterations in proto-oncogenes, dna repair genes, and tumor suppressor genes. loss of heterozygosity (loh) analysis has been used successfully to identify the genetic determinants of neoplastic development, including tumor suppressor genes, in several species and organs but not in the rat liver. we report the results of a sensitive genome-wide loh analysis of rat hepatocellular carcinomas ... | 2000 | 10820488 |
| neisseria gonorrhoeae ms11mkc opacity protein expression in vitro and during human volunteer infectivity studies. | neisseria gonorrhoeae ms11mkc harbors 11 independently expressed opacity (opa) protein genes with distinct in vitro expression frequencies. in experimental infections in which human male volunteers were inoculated with transparent (opa), piliated (p+) strains, the authors associate onset of symptoms with recovery of opaque (opa+) gonococci. | 2000 | 10821601 |
| dose response of ethanol on antioxidant defense system of liver, lung, and kidney in rat. | this study investigated the alterations in levels of glutathione, lipid peroxidation, and antioxidant enzyme activity in the liver, lung, and kidney of rats treated with acute doses of ethanol. male fisher-344 rats were randomly divided into four groups, and were treated as follows: (1) vehicle (saline) control; (2) ethanol 2 g/kg, p.o.; (3) ethanol 4g/kg, p.o.; and (4) ethanol 6 g/kg, p.o. the animals were sacrificed 1 h after treatment, and tissues were isolated and analyzed. the hepatic gsh l ... | 2000 | 10825682 |
| the role of care in nutrition programmes: current research and a research agenda. | the importance of cultural and behavioural factors in children's nutrition, particularly with regard to feeding, has been recognized only recently. the combination of evidence regarding the importance of caregiving behaviour for good nutrition, and improved strategies for measuring behaviour have led to a renewed interest in care. the unicef conceptual framework suggests that care, in addition to food security and health care services, are critical for children's survival, growth and development ... | 2000 | 10828171 |
| egf receptors of hepatocytes from rats treated with phenobarbital are sensitized to down-regulation by phenobarbital in culture. | hepatocytes from fisher 344 rats treated with the liver tumor promoter phenobarbital (phb; 0.1% in the drinking water, 2-3 months) exhibit reduced epidermal growth factor (egf) binding and egf-induced mitogenesis in culture. similar responses are induced by >1 mm phb added to the culture medium of hepatocytes from untreated rats. in this study, we demonstrated that hepatocyte egfr protein, as determined by immunoblotting, was unchanged by treatment of rats with phb. however, hepatocytes from phb ... | 2000 | 10828207 |
| major strain differences in response to chronic systemic administration of the mitochondrial toxin 3-nitropropionic acid in rats: implications for neuroprotection studies. | chronic systemic treatment with 3-nitropropionic acid in rats produces persistent dystonia and bradykinesia, and striatal lesions reminiscent of huntington's disease. however, the interpretation of results obtained with this model are complicated by a heterogeneous distribution of the response to a given toxic dose of 3-nitropropionic acid: approximately half of the animals develop selective striatal lesions, which in certain cases are associated with extrastriatal lesions, and the other half ar ... | 2000 | 10828534 |
| prognostic value of mib-1 antibody labeling index to predict response to bacillus calmette-guérin therapy in a high-risk selected population of patients with stage t1 grade g3 bladder cancer. | proliferation rate is an important determinant of bladder tumor progression. however, this factor has not yet been correlated to bacillus calmette-guérin (bcg) therapy response in a selected high-risk population of patients with stage t1 grade g3 bladder cancer. to assess the predictive value of the proliferation rate, an immunoreactivity test with monoclonal antibodies mib-1 was carried out. the aim of this study was to evaluate the prognostic value of an mib-1 labeling index by selecting a gro ... | 2000 | 10828663 |
| oxygen and placental development during the first trimester: implications for the pathophysiology of pre-eclampsia. | during early pregnancy, placentation occurs in a relatively hypoxic environment which is essential for appropriate embryonic development. intervillous blood flow increases at around 10-12 weeks of gestation and results in exposure of the trophoblast to increased oxygen tension (po2). prior to this time, low oxygen appears to prevent trophoblast differentiation towards an invasive phenotype. in other mammalian systems, oxygen tension effects are mediated by hypoxia inducible factor-1 (hif-1). we ... | 2000 | 10831118 |
| biological adaptation and social behaviour. | in 1930, both fisher and wright identified darwin's initial concept of adaptive evolution in the light of the genetical theory with intergenerational variation in allelic frequencies brought about by the action of natural selection through differential reproduction. they emphasized that selection only works at the level of the individual and that its only consequence is to increase fitness. one genetical evolution not easy to explain on these bases was that of social behaviour because any altrui ... | 2000 | 10834287 |
| molecular characterization of campylobacter jejuni from patients with guillain-barré and miller fisher syndromes. | campylobacter jejuni has been identified as the predominant cause of antecedent infection in guillain-barré syndrome (gbs) and miller fisher syndrome (mfs). the risk of developing gbs or mfs may be higher after infection with specific c. jejuni types. to investigate the putative clonality, 18 gbs- or mfs-related c. jejuni strains from the netherlands and belgium and 17 control strains were analyzed by serotyping (penner and lior), restriction fragment length polymorphism analysis of pcr products ... | 2000 | 10834992 |
| the association of asymptomatic bacterial vaginosis with upper genital tract infection. | objective: to determine whether asymptomatic bacterial vaginosis (bv) is associated with upper genital tract inflammation or bacterial colonization.methods: fifty nonpregnant women with intact uteri who planned to undergo gynecologic surgery and had no recent symptoms of vaginal infection were enrolled. we obtained a vaginal swab for gram stain, endocervical swab for chlamydia and gonorrhea dna probe testing, and pipelle endometrial biopsy for aerobic and anaerobic cultures and histology. we cor ... | 1998 | 10838276 |
| fibronectin and malignant disease-associated dna-binding protein 2 in hepatocarcinogenesis in rats. | changes in the plasma concentration of malignant disease-associated dna-binding protein 2 (mad2) and in the distribution of fibronectin and mad2 in liver tissue were studied in fisher-344 rats with diethylnitrosamine-induced hepatocarcinogenesis. the concentration of plasma mad2 significantly increased as pre-cancerous lesions developed into hepatocellular carcinoma. we believe that the increased plasma concentration of mad2 is caused by an increase in the degradation of fibronectin within hepat ... | 2000 | 10840943 |
| renal allograft protection with losartan in fisher-->lewis rats: hemodynamics, macrophages, and cytokines. | we sought to assess the effects of angiotensin receptor blockade on glomerular hypertension, macrophage recruitment, and cytokine expression, all of which contribute to the development of chronic graft injury in this model. | 2000 | 10844632 |
| association between postoperative outcome and results of magnetic resonance imaging and computed tomography in working dogs with degenerative lumbosacral stenosis. | to determine whether results of magnetic resonance imaging (mri) and computed tomography (ct) are associated with postoperative outcome in working dogs with degenerative lumbosacral stenosis. | 2000 | 10844969 |
| participation of endothelial cells in the development of glomerulosclerosis: a study on murine serum sickness nephritis with mitomycin c. | to investigate the participation of endothelial cells in glomerulosclerosis, the study was performed in serum sickness nephritis (ssn) with administration of mitomycin c (mmc). ssn was induced in 8 week male fisher rats by sensitizing them with albumin, chicken egg (ea). then mmc (0.5 mg/kg bodyweight) was injected daily for 3 days and they were killed at 1, 2, 4 and 6 week intervals. significant mesangial expansion and sclerosis were observed in the experimental mixed ssn-mmc group in compariso ... | 1996 | 10846567 |
| induction of metallothionein synthesis with preservation of testicular function in rats following long term renal transplantation. | metallothionein (mt), as an acute phase or stress-response protein and free radical scavenger, is related to inflammation and cellular protection from oxidative damage. in order to evaluate long-term testicular damage and the role of mt following renal transplant, nine allogenic (fisher 344 --> lewis) and seven isogenic (lewis --> lewis) renal transplants were performed and the recipient rats were followed for 140 days when allografts develop chronic transplant rejection. testicular weight, ligh ... | 2000 | 10850631 |
| the effects of processing and low dose irradiation on cortical bone grafts. | the authors studied the effects of standard processing and preprocessing low dose gamma irradiation (1.5 mrad) on the strength and incorporation of syngeneic and allogeneic cortical bone grafts. bilateral femoral middiaphyseal 8-mm segmental defects in 120 male fisher rats were stabilized with internal fixation. each defect received one of six types of grafts: fresh syngeneic, processed syngeneic, irradiated processed syngeneic, fresh allogeneic, processed allogeneic, and irradiated processed al ... | 2000 | 10853179 |
| serum leptin response to endogenous hyperinsulinemia in aging rats. | to determine if aging is associated with altered serum leptin response to diet-induced changes in endogenous hyperinsulinemia, male fisher 344 (f344) rats at different age groups were studied while on regular rat chow and following 10 days of experimental diets consisting of 60% of the weight as fructose or glucose. the serum leptin concentration (ng/ml) gradually increased from basal levels of 2.5+/-0.1 at age of 4 months to 3.7+/-0.1, 6.9+/-0.9, 9. 4+/-0.3 and 8.9+/-1.1 at 6, 12, 18 and 24 mon ... | 2000 | 10854632 |
| immunolocalization of stress proteins and extracellular matrix proteins in the rat tibia. | stress proteins (heat shock proteins [hsps]) serve a number of protective functions, including protection from apoptosis and acting as chaperones during protein biosynthesis. for example, hsp 27 has been defined as a chaperone for the g3 domain of aggrecan, while hsp 47 is the chaperone for type i collagen. separate cytoprotective roles for hsp 27 and hsp 70 have been demonstrated. the aim of this study was to define the expression of hsps in osteoblastic and chondrocytic cells of the growing ra ... | 2000 | 10855699 |
| decreased levels of proteasome activity and proteasome expression in aging spinal cord. | neuron death and neuron degeneration occur in the cns during the course of aging. although multiple cellular alterations transpire during the aging process, those that mediate age-associated neuron death have not been identified. recent evidence implicates oxidative stress as a possible means of neuron death and neuron degeneration during aging. in the present study, we demonstrate a marked decrease in multicatalytic proteasome activity in the spinal cord of fisher 344 rats at 12, 24 and 28 mont ... | 2000 | 10858621 |
| differences in the response to oxidative stress and mutant frequency in cd (sprague-dawley) and fisher 344 rats due to an induced inflammatory response. | in this study, the rodent air pouch model was used to examine the production and processing of oxidative dna damage in two strains of rats commonly used in toxicity testing. an inflammatory response was induced by injecting zymosan a (50 mg) into an air pouch on male cd (sprague-dawley [s-d]) and fisher 344 (f-344) rats, and the animals were then sacrificed at 1, 3, 7, 14, and 28 days (n = 6 per time point per strain). tissues from the lining of the air pouch were collected for 8-hydroxy-2'-deox ... | 2000 | 10861952 |
| a role for oxidative stress in suppressing serum immunoglobulin levels in lead-exposed fisher 344 rats. | evidence implicating oxidative stress in toxicity during lead intoxication in vivo has opened new avenues for investigation of the mechanisms of lead-induced immunosuppression. the current study explores the possibility that lead-induced oxidative stress contributes to the immunosuppression observed during lead poisoning. fisher 344 rats were exposed to 2,000 ppm lead acetate in their drinking water for 5 weeks. one week following removal of lead from the drinking water, significant reductions i ... | 2000 | 10871428 |
| performance of a group b streptococcal prophylaxis protocol combining high-risk treatment and low-risk screening. | this study was undertaken to evaluate a group b streptococcal protocol in a large community hospital that combined treatment of high-risk patients with rapid screening of low-risk patients. | 2000 | 10871447 |
| treatment of inflammatory bowel disease in a rodent model with the intestinal growth factor glucagon-like peptide-2. | microinjection of a fisher (f344) rat zygote with human hla-b27 and beta2-microglobulin genes induces spontaneous chronic gastrointestinal (gi) inflammation similar to lesions seen in patients with inflammatory bowel disease (ibd). this study was designed to evaluate the potential therapeutic benefit of glp-2, an intestinal growth factor, in this transgenic rat model of ibd. | 2000 | 10873024 |
| dermal penetration and metabolism of five glycidyl ethers in human, rat and mouse skin. | 1. glycidyl ethers (ge), an important class of industrial chemicals, are considered to be potentially mutagenic in vivo because some ge have been shown to be direct mutagens in short-term in vitro tests. 2. the percutaneous penetration and metabolism of representatives of different classes of ge was studied in the fresh, full-thickness c3h mouse, and dermatomed human and fisher 344 rat skin to determine the apparent permeability constants, lag times and metabolic profiles. 3. five different ge, ... | 2000 | 10875681 |
| [chemopreventive effect of tamoxifen on familial breast cancer]. | chemopreventive effect of tamoxifen on familial breast cancer was studied. as it is impossible to use medicines for prevention of human breast cancer in japan, a lot of investigations on animals using natural foods like as green tea, seaweed etc has been done. seaweed wakame was effective on breast cancer proliferation in rat. in this paper 3 reports(fisher, powles and veronesi) were reviewed and the results of some japanese investigations in chemoprevention on animals were introduced. in fisher ... | 2000 | 10879052 |
| treatment of rat experimental brain tumors by herpes simplex virus thymidine kinase gene-transduced allogeneic tumor cells and ganciclovir. | transfer of the herpes simplex virus thymidine kinase (hsvtk) gene, followed by administration of ganciclovir (gcv), generates the "bystander effect," in which hsvtk-negative wild-type cells are killed by gcv, as are hsvtk-expressing cells. our previous study demonstrated that intracranial 9l gliomas could be efficiently treated due to this bystander effect by injecting the 9l glioma cells transduced with the hsvtk gene in the vicinity of the preimplanted wild-type 9l glioma and then administeri ... | 2000 | 10880027 |
| anti-gq1b antibodies and evoked acetylcholine release at mouse motor endplates. | miller fisher syndrome (mfs) is clinically characterized by ataxia, areflexia, and ophthalmoplegia, and is associated with serum anti-gq1b-ganglioside antibodies. we have previously shown that anti-gq1b antibodies induce complement-dependent, alpha-latrotoxin-like effects at mouse neuromuscular junctions (nmjs) in vitro. this effect comprises a massive increase in spontaneous quantal acetylcholine (ach) release, accompanied by block of evoked release and muscle paralysis. this mechanism may cont ... | 2000 | 10882997 |
| hirudin reduces tissue factor expression and attenuates graft arteriosclerosis in rat cardiac allografts. | background-intravascular clotting has been implicated in the pathogenesis of cardiac allograft vasculopathy (cav). we previously identified the expression of tissue factor (tf), the primary cellular initiator of blood coagulation, within the coronary intima, which was associated with neointimal thickening. in the present study, the effect of recombinant hirudin on cav was assessed in lewis to fisher rat heterotopic cardiac allografts. methods and results-transplant recipients were randomized to ... | 2000 | 10899102 |
| costly signaling and torch fishing on ifaluk atoll. | in this paper i evaluate the merit of costly signaling theory (cst) as a paradigm for understanding why men of ifaluk atoll torch fish. i argue that torch fishing is a handicap that signals men's productivity. consistent with cst, torch fishing is observed by the predicted audience (women), energetically costly to perform, and a reliable indicator of the frequency a man fishes during the trade wind season. contrary to expectations of who should benefit from torch fishing and consequently partici ... | 2000 | 10899476 |
| effects of dextroamphetamine on depression and fatigue in men with hiv: a double-blind, placebo-controlled trial. | this report documents findings from a small placebo-controlled trial of dextroamphetamine for depression and fatigue in men with the human immunodeficiency virus (hiv). dextroamphetamine offers the potential for rapid onset of effect and activation properties, both of which are important to persons with medical illness and an uncertain, but limited, life expectancy. | 2000 | 10901342 |
| basal and drug-induced antioxidant enzyme activities correlate with age-dependent doxorubicin oxidative toxicity. | doxorubicin continues to be one of the most widely used anticancer agents in the clinic despite its dose-limiting side-effects. many of doxorubicin's dose-limiting toxicities occur due to its generation of toxic oxygen species, resulting in oxidative stress. some clinical observations have suggested that doxorubicin may have greater toxicity in older patients. the studies presented here compare basal and doxorubicin-induced antioxidant enzyme activities in brain, heart, kidney and liver tissues ... | 2000 | 10903415 |
| chagas disease and human migration. | human chagas disease is a purely accidental occurrence. as humans came into contact with the natural foci of infection might then have become infected as a single addition to the already extensive host range of trypanosoma cruzi that includes other primates. thus began a process of adaptation and domiciliation to human habitations through which the vectors had direct access to abundant food as well as protection from climatic changes and predators. our work deals with the extraction and specific ... | 2000 | 10904414 |
| megalin-mediated transcytosis of thyroglobulin by thyroid cells is a calmodulin-dependent process. | megalin, a multiligand receptor expressed on the apical surface of thyroid cells, mediates transepithelial transport (transcytosis) of thyroglobulin (tg) across thyrocytes, resulting in diversion of tg from the lysosomal pathway and reduction of the extent of thyroid hormone release from internalized tg molecules. the calcium regulatory protein calmodulin facilitates some forms of transcytosis. here we investigated the role of calmodulin in megalin-mediated transcytosis of tg by thyroid cells. f ... | 2000 | 10907988 |
| contrasting effects of oncogene expression on two carrier-mediated systems internalizing folate compounds in fisher rat 3t3 cells. | folate compound transport into fisher rat 3t3 (fr3t3) cells at physiological ph occurs predominantly by an acid ph-dependent, mobile carrier system. however, influx of [(3)h]mtx by this system is 3-4-fold higher at ph 6 than at ph 7.5, the optimum for rfc-1-mediated folate compound transport. this acid ph dependency reflects an alteration of influx v(max) rather than of influx k(m) in these cells at different ph. acid ph-dependent folate compound transport interacts effectively with mtx, 5llcho- ... | 2000 | 10911368 |
| impaired bax protein expression in breast cancer: mutational analysis of the bax and the p53 gene. | we have previously shown that the pro-apoptotic bax protein is differentially expressed in breast cancer and in other epithelial tumors. in this line, a reduced bax protein expression is a negative prognostic factor in various carcinomas including breast cancer. for p53, a trancriptional activator of bax in apoptosis, mutations in the coding sequence were shown to modulate bax protein expression in cell line models on the transcriptional level. we therefore investigated the bax gene in 68 breast ... | 2000 | 10918191 |
| x-irradiation causes a prolonged reduction in cell proliferation in the dentate gyrus of adult rats. | the effects of x-irradiation on proliferating cells in the dentate subgranular zone were assessed in young adult fisher 344 rats exposed to a range of x-ray doses and followed for up to 120 days. apoptosis was quantified using morphology and end-labeling immunohistochemistry, and cell proliferation was detected using antibodies against the thymidine analog brdu and the cyclin-dependent kinase p34(cdc2). radiation-induced apoptosis occurred rapidly, with maximum morphological and end-labeling cha ... | 2000 | 10924950 |
| 1,3-butadiene: cancer, mutations, and adducts. part ii: roles of two metabolites of 1,3-butadiene in mediating its in vivo genotoxicity. | 1,3-butadiene (bd) is carcinogenic in mice and rats, with mice being more susceptible than rats to its carcinogenic effects. 1,3-butadiene is mutagenic in the bone marrow and spleen cells of b6c3f1 laci transgenic mice. the goal of this research was to assess the roles of two bd metabolites, 1,2-epoxy-3-butene (bdo) and 1,2,3,4-diepoxybutane (bdo2), in the mutagenicity and mutational spectrum of the parent compound bd by determining the mutagenicity and mutational spectra of bdo and bdo2 in huma ... | 2000 | 10925839 |
| morphogenesis of craniopharyngeal derivatives in the neurohypophysis of fisher 344 rats: abnormally developed epithelial tissues including parotid glands derived from the stomatodeum. | morphogenesis of craniopharyngeal derivatives of the neurohypophysis found in 14 fischer 344 (f344) rats was studied. the incidence of the craniopharyngeal derivatives was 0.17% in male (7 out of 4,200) and 0.16% in female (7 out of 4,450) f344 rats. neither a sex-related difference in their incidence nor a strain-related difference in their morphological features was observed. craniopharyngeal derivatives were composed of aberrant epithelial structures consisting of serous acinar and tubular an ... | 2000 | 10930044 |
| phase variation of a beta-1,3 galactosyltransferase involved in generation of the ganglioside gm1-like lipo-oligosaccharide of campylobacter jejuni. | ganglioside mimicry by campylobacter jejuni lipo-oligosaccharide (los) is thought to be a critical factor in the triggering of the guillain-barré and miller-fisher syndrome neuropathies after c. jejuni infection. the combination of a completed genome sequence and a ganglioside gm1-like los structure makes c. jejuni nctc 11168 a useful model strain for the identification and characterization of the genes involved in the biosynthesis of ganglioside-mimicking los. genome analysis identified a putat ... | 2000 | 10931344 |
| improved recovery following posttransplant acute renal failure in rat renal isografts with an oral endothelin-a receptor antagonist. | delayed renal function after transplantation is a strong predictor of long-term graft survival. as an increased expression of endothelin (et) has been demonstrated during ischemia/reperfusion injury, we hypothesized that et-a receptor blockade could improve the recovery of acute renal failure in a rat model of isogeneic kidney transplantation. | 2000 | 10940728 |
| recombinant staphylokinase variants with reduced antigenicity due to elimination of b-lymphocyte epitopes. | site directed mutagenesis (350 variants) of recombinant staphylokinase (sakstar), a potent fibrin-selective thrombolytic agent, was undertaken in order to reduce its antigenicity while maintaining its potency. variants with k35a, (ie, lys[k] in position 35 substituted with ala[a]), e65d or e65q, k74r or k74q, e80a+d82a, k130t, and k135r displayed increased enzymatic activity or reduced binding of human staphylokinase-specific antibodies. additive mutagenesis identified 8 variants with intact thr ... | 2000 | 10942387 |
| vaginal infections in human immunodeficiency virus-infected women. | this study was undertaken to compare the frequencies of vaginal infections among human immunodeficiency virus-infected women with those among human immunodeficiency virus-seronegative women. | 2000 | 10942468 |
| late third-trimester treatment of rectovaginal group b streptococci with benzathine penicillin g. | we sought to determine the efficacy of late third-trimester benzathine penicillin g administration in eradicating maternal group b streptococcal colonization at delivery. | 2000 | 10942472 |
| the use of entomopathogenic fungi for the control of parasitic mites, psoroptes spp. | in vitro trials were carried out to evaluate the potential of fungal pathogens as biological control agents of parasitic mites, psoroptes ovis (hering) (acari: psoroptidae) from rabbit hosts (synonym: psoroptes cuniculi). the fungus hirsutella thompsonii fisher showed no pathogenicity. metarhizium anisopoliae (metschinkoff), however, showed a high level of pathogenicity; 3 days after exposure to fungal conidia all mites were dead and 6 days after exposure 60% of the dead adult females, 10% of th ... | 2000 | 10946133 |
| is campylobacter lipopolysaccharide bearing a gd3 epitope essential for the pathogenesis of guillain-barré syndrome? | the hypothesis has been proposed that the gd3 ganglioside-like lipopolysaccharide (lps) is essential for and functions in the development of guillain-barré syndrome (gbs) and miller fisher syndrome (mfs) subsequent to campylobacter jejuni enteritis. our study showed that patients with gbs or mfs who had previously suffered diarrhea had anti-gd3 antibodies less often than those who had not had diarrhea. sera from patients who showed gbs or mfs with the serologic evidence of prior c. jejuni infect ... | 2000 | 10949532 |
| binding of rat thyroglobulin to heparan sulfate proteoglycans. | we previously showed that rat thyroglobulin (tg) is a heparin-binding protein and that heparin inhibits tg binding to megalin (gp330), an endocytic tg receptor found on the apical surface of thyrocytes. cooperation between cell surface receptors and heparin-like molecules, namely heparan sulfate proteoglycans (hspgs), can facilitate cell surface binding of some heparin-binding proteins. based on our previous findings indicating that heparin and megalin-binding sites of rat tg are functionally re ... | 2000 | 10958307 |
| selenium from high selenium broccoli protects rats from colon cancer. | colon cancer is the third most common newly diagnosed cancer in the united states and the third most common cause of cancer-related deaths. previous supplementation studies have demonstrated the efficacy of selenium (se) for prevention of colon cancer in humans. the metabolism of se depends on its chemical form, and studies have shown that the chemical form of se in broccoli does not accumulate in the body as fast as other forms of se and may be especially beneficial for prevention of cancer. in ... | 2000 | 10958840 |
| effects of aging on nerve conduction block induced by bupivacaine and procaine in rats. | to test the hypothesis that the dose requirement for local anesthetics is changed in aged animals, the effects of two different local anesthetics on nerve conduction block were tested in young and old rats. young (6 months) and old (27 months) male fisher-344 rats were anesthetized with intraperitoneal pentobarbital and diazepam. stimulating electrodes were placed in the sciatic notch and in the ankle and recording electrodes were placed distally in the ipsilateral foot to record evoked electric ... | 1997 | 10959231 |
| amplified fragment length polymorphism analysis of campylobacter jejuni strains isolated from chickens and from patients with gastroenteritis or guillain-barré or miller fisher syndrome. | the high-resolution genotyping method of amplified fragment length polymorphism (aflp) analysis was used to study the genetic relationships between campylobacter jejuni strains infecting chickens (n = 54) and those causing gastroenteritis in humans (n = 53). in addition, c. jejuni strains associated with the development of guillain-barré syndrome (gbs) (n = 14) and miller fisher syndrome (mfs) (n = 4), two related acute paralytic syndromes in human, were included. strains were isolated between 1 ... | 2000 | 10966409 |
| vascular beta-adrenergic receptor adenylyl cyclase system in maturation and aging. | the objective of this study was to determine how maturation and aging affects beta (beta)-adrenergic receptor (ar) control of arterial vasorelaxation. left ventricular (lv) hemodynamics and arterial vasorelaxation in thoracic artery segments were studied in brown norway, fisher 344 cross rats at 6 weeks, 6 months, and 23 months of age. we defined changes in maturation as occurring between 6 weeks and 6 months of age and changes in aging as occurring between 6 months and 23 months of age. with ma ... | 2000 | 10966835 |
| a magnetic field exposure facility for evaluation of animal carcinogenicity. | several animal studies have been carried out at the institut armand frappier (iaf) to determine whether chronic exposure to 60 hz linearly polarized sinusoidal magnetic fields might increase the risk of cancer development of female fisher rats. the magnetic field exposure facility was developed to meet the requirements of the study protocol for chronic exposure of large number of animals to field intensities of sham < 0.2 microt, 2 microt, 20 microt, 200 microt, and 2000 microt. at each exposure ... | 2000 | 10972947 |
| kinematics of the hind limb in trotting horses after induced lameness of the distal intertarsal and tarsometatarsal joints and intra-articular administration of anesthetic. | to identify hind limb and pelvic kinematic variables that change in trotting horses after induced lameness of the distal intertarsal and tarsometatarsal joints and after subsequent intra-articular administration of anesthetic. | 2000 | 10976732 |
| experimental hyperprolactinemia in a rat model: alteration in centrally mediated neuroerectile mechanisms. | the purpose of this study was to induce hyperprolactinemia in adult male rats and determine its effect on the central and peripheral mediated neurogenic as well as myogenic mechanisms of erectile function/dysfunction in a rat model. the use of penile reflexes in awake animals was used to test the effect of both central and peripheral mechanisms. electrical stimulation of the cavernous nervous was used to test peripheral neural function and intracorporal injection of papaverine was used to test t ... | 2000 | 10982309 |
| p53 polymorphism, human papillomavirus infection in the oral cavity, and oral cancer. | human papillomavirus (hpv) infection has emerged as a risk factor in oral carcinogenesis. an arginine-coding polymorphism of the tumor suppressor protein p53 at codon 72 is more readily degraded by the hpv oncoprotein e6. our objective was to evaluate the association between p53 polymorphism at codon 72 and hpv infection in the oral cavity, as well as its association with oral cancer. study design: oral squamous cells from 202 patients with oral cancer and 333 age-sex frequency matched controls ... | 2000 | 10982955 |
| [clinical picture, evolution and results of treatment of guillain-barré syndrome in children and adolescents]. | 68 patients with guillain-barré syndrome, 36 girls and 32 boys at the age of 12 months up to 18 years were treated in the department of developmental neurology in poznań in 1989-1999. the incidence of neurological symptoms was preceded in 50 patients (74%) by the infection of the alimentary or upper respiratory tracts. however, only 39 (57%) presented virus etiology and moreover, 7 children out of this group showed significant growth of antibodies to poliovirus. six-degree range was applied to e ... | 2000 | 10983298 |
| an animal model for the study of genetic predisposition in the pathogenesis of middle ear inflammation. | chronic otitis media with effusion (come) is the most prevalent inflammatory disease in children and is associated with numerous adverse long-term sequelae. many factors have been associated with an increased risk of developing come, one of which may be a genetic predisposition to the disease. to study the role that genetics play in the pathogenesis of come, we used an animal model to compare the middle ear inflammatory responses in two different strains of rats (lewis and fisher). | 2000 | 10983952 |
| differential fos expression in the paraventricular nucleus of the hypothalamus, sacral parasympathetic nucleus and colonic motor response to water avoidance stress in fischer and lewis rats. | the responsiveness of hypothalamic crf to various stressors is reduced in the young female lewis relative to the histocompatible fischer rat. whether such a difference impacts the brain-gut response to water avoidance stress was investigated by monitoring fos immunoreactivity in the brain and sacral spinal cord and fecal pellet output. exposure for 60 min to water avoidance stress increased the number of fos positive cells in the paraventricular nucleus of the hypothalamus (pvn), nucleus tractus ... | 2000 | 10986349 |
| comparison of the water-holding capacity of wheat bran products prepared by wet and dry smashing methods in vitro and effect on the gastrointestinal retention time in rats in vivo. | microfibril wheat bran (mfw) prepared by wet smashing of wheat bran using a colloidal mill has the advantages of being more palatable than other wheat bran and easier to apply to various foods. in this study, we investigated water-holding capacity (whc) and physiological effects of a novel food material, mfw, focusing on shortening of the retention time of the gastrointestinal contents compared to those of dry smashing of wheat bran (dwb) prepared by conventional method, and wheat bran (wb), whi ... | 2000 | 10989767 |
| monoamine neurotransmitters in resected hippocampal subparcellations from neocortical and mesial temporal lobe epilepsy patients: in situ microvoltammetric studies. | it is known that epilepsy patients diagnosed with neocortical temporal lobe epilepsy (ntle), differ from those diagnosed with mesial temporal lobe epilepsy (mtle), e.g., in hippocampal (hpc) pathology. in the present studies, we tested the hypothesis that ntle and mtle subtypes of human epilepsy might differ in regards to their hpc monoamine neurochemistry. monoamine neurotransmitters were studied in separate signals and within s with semiderivative microvoltammetry, used in combination with ste ... | 2000 | 10996135 |
| expression of cyclooxygenase-1 and -2 mrna in rat tissues: tissue-specific difference in the expression of the basal level of mrna. | the expression of cyclooxygenase (cox)-1 and -2 mrna was studied in tissues of fisher 344 (f344) (n=3) and brown norway (bn) (n=3) strain rats using the reverse transcription polymerase chain reaction. cox-1 mrna was expressed in the liver, kidney and lung, but not in the heart. cox-2 mrna was expressed in the kidney, heart and lung, but not in the liver. the present results showed the differential expression of basal cox-1 and cox-2 mrna in the rat tissues, suggesting that cox-1 and cox-2 may c ... | 2000 | 10998438 |
| [a case report of bickerstaff's brainstem encephalitis with positive anti gq 1 b, gt 1 a, gm 1 ganglioside antibodies]. | patient was an 18-year-old female student. after she had symptoms of common cold for 3 days, she developed somnolence, diplopia, dysarthria, urinary disturbance and ataxia. on admission neurological examination revealed coma with mydriasis, ophthalmoplegia, ptosis and weakness of the upper limbs. light reflex, corneal reflex and oculocephalic test were all negative. deep tendon reflexes were brisk and extensor toe signs were positive bilaterally. she did not have nuchal rigidity. laboratory test ... | 2000 | 11002482 |
| cell transplantation causes loss of gap junctions and activates ggt expression permanently in host liver. | cell transplantation into hepatic sinusoids, which is necessary for liver repopulation, could cause hepatic ischemia. to examine the effects of cell transplantation on host hepatocytes, we transplanted fisher 344 rat hepatocytes into syngeneic dipeptidyl peptidase iv-deficient rats. within 24 h of cell transplantation, areas of ischemic necrosis, along with transient disruption of gap junctions, appeared in the liver. moreover, host hepatocytes expressed gamma-glutamyl transpeptidase (ggt) exten ... | 2000 | 11005770 |
| effect of adhesive retention on maxillofacial prostheses. part i: skin dressings and solvent removers. | the success of most maxillofacial prostheses depends on retention by medical adhesives. products such as smith-nephew's skin-prep (sp) are available that can be used on the skin that could improve prosthesis adhesion protective dressing. the removal of adhesive from the skin is also problematic, so solvents, such as uni-solve adhesive remover (us), are often used. | 2000 | 11005907 |
| urokinase-type plasminogen activator and its receptor in colorectal cancer: independent prognostic factors of metastasis and cancer-specific survival and potential therapeutic targets. | urokinase-type plasminogen activator (upa) and its receptor (upar), plasminogen (plg), and plasminogen activator inhibitors-1 and -2 (pai-1 and pai-2) have been observed in many cancers and may contribute to progression and metastasis. in our study, we examined the expression of the 5 proteins by immunohistochemistry in 59 consecutive primary colorectal cancers (crc) and correlated the protein expression with patient outcome. in addition, we determined the effect of down-regulation of upar on th ... | 2000 | 11008205 |