| structural understanding of the recycling of oxidized ascorbate by dehydroascorbate reductase (osdhar) from oryza sativa l. japonica. | dehydroascorbate reductase (dhar) is a key enzyme involved in the recycling of ascorbate, which catalyses the glutathione (gsh)-dependent reduction of oxidized ascorbate (dehydroascorbate, dha). as a result, dhar regenerates a pool of reduced ascorbate and detoxifies reactive oxygen species (ros). in previous experiments involving transgenic rice, we observed that overexpression of dhar enhanced grain yield and biomass. since the structure of dhar is not available, the enzymatic mechanism is not ... | 2016 | 26775680 |
| expression of zinc transporter genes in rice as influenced by zinc-solubilizing enterobacter cloacae strain zsb14. | zinc (zn) deficiency in major food crops has been considered as an important factor affecting the crop production and subsequently the human health. rice (oryza sativa) is sensitive to zn deficiency and thereby causes malnutrition to most of the rice-eating asian populations. application of zinc solubilizing bacteria (zsb) could be a sustainable agronomic approach to increase the soil available zn which can mitigate the yield loss and consequently the nutritional quality of rice. understanding t ... | 2016 | 27092162 |
| differential expression of cpks and cytosolic ca2+ variation in resistant and susceptible apple cultivars (malus x domestica) in response to the pathogen erwinia amylovora and mechanical wounding. | plant calcium (ca2+) signals are involved in a wide array of intracellular signalling pathways following pathogen invasion. ca2+-binding sensory proteins such as ca2+-dependent protein kinases (cpks) have been predicted to mediate signalling following ca2+ influx after pathogen infection. however, to date this prediction has remained elusive. | 2013 | 24192013 |
| gene duplication in the carotenoid biosynthetic pathway preceded evolution of the grasses. | despite ongoing research on carotenoid biosynthesis in model organisms, there is a paucity of information on pathway regulation operating in the grasses (poaceae), which include plants of world-wide agronomic importance. as a result, efforts to either breed for or metabolically engineer improvements in carotenoid content or composition in cereal crops have led to unexpected results. in comparison to maize (zea mays), rice (oryza sativa) accumulates no endosperm carotenoids, despite having a func ... | 2004 | 15247400 |
| phytoene desaturase from oryza sativa: oligomeric assembly, membrane association and preliminary 3d-analysis. | recombinant phytoene desaturase (pds-his6) from rice was purified to near-homogeneity and shown to be enzymatically active in a biphasic, liposome-based assay system. the protein contains fad as the sole protein-bound redox-cofactor. benzoquinones, not replaceable by molecular oxygen, serve as a final electron acceptor defining pds as a 15-cis-phytoene (donor):plastoquinone oxidoreductase. the herbicidal pds-inhibitor norflurazon is capable of arresting the reaction by stabilizing the intermedia ... | 2015 | 26147209 |
| draft genome sequence of the plant pathogen dickeya zeae dz2q, isolated from rice in italy. | dickeya zeae is an emerging rice (oryza sativa) pathogen causing bacterial foot rot. related pathogens affect maize (zea mays) and potato (solanum tuberosum) and a variety of important ornamental and floral plants. here, we present the draft genome sequence of d. zeae dz2q, an isolate obtained from rice grown in italy. | 2013 | 24201194 |
| determination of supplier-to-supplier and lot-to-lot variability in glycation of recombinant human serum albumin expressed in oryza sativa. | the use of different expression systems to produce the same recombinant human protein can result in expression-dependent chemical modifications (cms) leading to variability of structure, stability and immunogenicity. of particular interest are recombinant human proteins expressed in plant-based systems, which have shown particularly high cm variability. in studies presented here, recombinant human serum albumins (rhsa) produced in oryza sativa (asian rice) (osrhsa) from a number of suppliers hav ... | 2014 | 25299339 |
| improving pharmaceutical protein production in oryza sativa. | application of plant expression systems in the production of recombinant proteins has several advantages, such as low maintenance cost, absence of human pathogens, and possession of complex post-translational glycosylation capabilities. plants have been successfully used to produce recombinant cytokines, vaccines, antibodies, and other proteins, and rice (oryza sativa) is a potential plant used as recombinant protein expression system. after successful transformation, transgenic rice cells can b ... | 2013 | 23615467 |
| rice endosperm is cost-effective for the production of recombinant griffithsin with potent activity against hiv. | protein microbicides containing neutralizing antibodies and antiviral lectins may help to reduce the rate of infection with human immunodeficiency virus (hiv) if it is possible to manufacture the components in large quantities at a cost affordable in hiv-endemic regions such as sub-saharan africa. we expressed the antiviral lectin griffithsin (grft), which shows potent neutralizing activity against hiv, in the endosperm of transgenic rice plants (oryza sativa), to determine whether rice can be u ... | 2016 | 26800650 |
| identification of drought-responsive genes in roots of upland rice (oryza sativa l). | rice (oryza sativa l.) germplasm represents an extraordinary source of genes that control traits of agronomic importance such as drought tolerance. this diversity is the basis for the development of new cultivars better adapted to water restriction conditions, in particular for upland rice, which is grown under rainfall. the analyses of subtractive cdna libraries and differential protein expression of drought tolerant and susceptible genotypes can contribute to the understanding of the genetic c ... | 2008 | 18922162 |
| population diversity of rice stripe virus-derived sirnas in three different hosts and rnai-based antiviral immunity in laodelphgax striatellus. | small rna-mediated gene silencing plays evolutionarily conserved roles in gene regulation and defense against invasive nucleic acids. virus-derived small interfering rnas (vsirnas) are one of the key elements involved in rna silencing-based antiviral activities in plant and insect. vsirnas produced after viruses infecting hosts from a single kingdom (i.e., plant or animal) are well described. in contrast, vsirnas derived from viruses capable of infecting both plants and their insect vectors have ... | 2012 | 23029445 |
| comparison of class 2 transposable elements at superfamily resolution reveals conserved and distinct features in cereal grass genomes. | class 2 transposable elements (tes) are the predominant elements in and around plant genes where they generate significant allelic diversity. using the complete sequences of four grasses, we have performed a novel comparative analysis of class 2 tes. to ensure consistent comparative analyses, we re-annotated class 2 tes in brachypodium distachyon, oryza sativa (rice), sorghum bicolor and zea mays and assigned them to one of the five cut-and-paste superfamilies found in plant genomes (tc1/mariner ... | 2013 | 23369001 |
| an improved 2b-rad approach (i2b-rad) offering genotyping tested by a rice (oryza sativa l.) f2 population. | 2b-rad (type iib endonucleases restriction-site associated dna) approach was invented by wang in 2012 and proven as a simple and flexible method for genome-wide genotyping. however, there is still plenty of room for improvement for the existent 2b-rad approach. firstly, it doesn't include the samples pooling in library preparation as other reduced representation libraries. secondly, the information of 2b-rad tags, such as tags numbers and distributions, in most of species are unknown. the purpos ... | 2014 | 25373334 |
| comparing low coverage random shotgun sequence data from brassica oleracea and oryza sativa genome sequence for their ability to add to the annotation of arabidopsis thaliana. | since the completion of the arabidopsis thaliana genome sequence, there is an ongoing effort to annotate the genome as accurately as possible. comparing genome sequences of related species complements the current annotation strategies by identifying genes and improving gene structure. a total of 595,321 brassica oleracea shotgun reads were sequenced by tigr (the institute for genome research) and the collaboration of washington university and cold spring harbor. vicogenta (a genome viewer based ... | 0 | 15805491 |
| transcription factor families have much higher expansion rates in plants than in animals. | transcription factors (tfs), which are central to the regulation of gene expression, are usually members of multigene families. in plants, they are involved in diverse processes such as developmental control and elicitation of defense and stress responses. to investigate if differences exist in the expansion patterns of tf gene families between plants and other eukaryotes, we first used arabidopsis (arabidopsis thaliana) tfs to identify tf dna-binding domains. these dna-binding domains were then ... | 0 | 16166257 |
| ltr retrotransposons in rice (oryza sativa, l.): recent burst amplifications followed by rapid dna loss. | ltr retrotransposons are one of the main causes for plant genome size and structure evolution, along with polyploidy. the characterization of their amplification and subsequent elimination of the genomes is therefore a major goal in plant evolutionary genomics. to address the extent and timing of these forces, we performed a detailed analysis of 41 ltr retrotransposon families in rice. | 2007 | 17617907 |
| predicting the function of 4-coumarate:coa ligase (lj4cl1) in lonicera japonica. | 4-coumarate:coa ligases (4cls) are a group of essential enzymes involved in the pathway of phenylpropanoid-derived compound metabolisms; however it is still difficult to identify orthologs and paralogs of these important enzymes just based on sequence similarity of the conserved domains. using sequence data of 20 plant species from the public databases and sequences from lonicera japonica, we define 1252 adenosine monophosphate (amp)-dependent synthetase/ligase sequences and classify them into t ... | 2014 | 24518682 |
| isolation and characterization of microsatellite loci in the asian rice gall midge (orseolia oryzae) (diptera: cecidomyiidae). | microsatellite loci were isolated from the genomic dna of the asian rice gall midge, orseolia oryzae (wood-mason) using a hybridization capture approach. a total of 90 non-redundant primer pairs, representing unique loci, were designed. these simple sequence repeat (ssr) markers represented di (72%), tri (15.3%), and complex repeats (12.7%). three biotypes of gall midge (20 individuals for each biotype) were screened using these ssrs. the results revealed that 15 loci were hyper variable and sho ... | 2011 | 21340012 |
| distinct gene expression profiles in egg and synergid cells of rice as revealed by cell type-specific microarrays. | double fertilization in flowering plants refers to a process in which two sperm cells, carried by the pollen tube, fertilize both the egg and the central cell after their release into a synergid cell of the female gametophyte. the molecular processes by which the female gametophytic cells express their unique functions during fertilization are not well understood. genes expressed in egg and synergid cells might be important for multiple stages of the plant reproductive process. here, we profiled ... | 2010 | 21106719 |
| golgi transport 1b regulates protein export from the endoplasmic reticulum in rice endosperm cells. | coat protein complex ii (copii) mediates the first step of anterograde transport of newly synthesized proteins from the endoplasmic reticulum (er) to other endomembrane compartments in eukaryotes. a group of evolutionarily conserved proteins (sar1, sec23, sec24, sec13, and sec31) constitutes the basic copii coat machinery; however, the details of how the copii coat assembly is regulated remain unclear. here, we report a protein transport mutant of rice (oryza sativa), named glutelin precursor ac ... | 2016 | 27803308 |
| modeling the evolution dynamics of exon-intron structure with a general random fragmentation process. | most eukaryotic genes are interrupted by spliceosomal introns. the evolution of exon-intron structure remains mysterious despite rapid advance in genome sequencing technique. in this work, a novel approach is taken based on the assumptions that the evolution of exon-intron structure is a stochastic process, and that the characteristics of this process can be understood by examining its historical outcome, the present-day size distribution of internal translated exons (exon). through the combinat ... | 2013 | 23448166 |
| eukaryotic genomes may exhibit up to 10 generic classes of gene promoters. | the main function of gene promoters appears to be the integration of different gene products in their biological pathways in order to maintain homeostasis. generally, promoters have been classified in two major classes, namely tata and cpg. nevertheless, many genes using the same combinatorial formation of transcription factors have different gene expression patterns. accordingly, we tried to ask ourselves some fundamental questions: why certain genes have an overall predisposition for higher ge ... | 2012 | 23020586 |
| extracellular membrane vesicles and phytopathogenicity of acholeplasma laidlawii pg8. | for the first time, the phytopathogenicity of extracellular vesicles of acholeplasma laidlawii pg8 (a ubiquitous mycoplasma that is one of the five common species of cell culture contaminants and is a causative agent for phytomycoplasmoses) in oryza sativa l. plants was studied. data on the ability of extracellular vesicles of acholeplasma laidlawii pg8 to penetrate from the nutrient medium into overground parts of oryza sativa l. through the root system and to cause alterations in ultrastructur ... | 2012 | 23251100 |
| genetic organization of the hrp gene cluster in acidovorax avenae strain n1141 and a novel effector protein that elicits immune responses in rice (oryza sativa l.). | the immune system of plants consists of two main arms, pathogen-associated molecular pattern (pamp)-triggered immunity (pti) and effector-triggered immunity (eti). the multiple effectors that trigger eti are translocated into plant cells by the type iii secretion system (t3ss) of pathogenic bacteria. the rice-avirulent n1141 strain of acidovorax avenae causes eti in rice, including hypersensitive response (hr) cell death. sequence analysis indicated that the n1141 genome contains the hrp gene cl ... | 2012 | 22232252 |
| production and immunogenicity of actinobacillus pleuropneumoniae apxiia protein in transgenic rice callus. | actinobacillus pleuropneumoniae is a major etiological agent that is responsible for swine pleuropneumonia, a highly contagious respiratory infection that causes severe economic losses in the swine production industry. apxiia is one of the virulence factors in a. pleuropneumoniae and has been considered as a candidate for developing a vaccine against the bacterial infection. a gene encoding an apxiia fragment (amino acids 439-801) was modified based on a plant-optimized codon and constructed int ... | 2016 | 27215671 |
| evaluation of four phloem-specific promoters in vegetative tissues of transgenic citrus plants. | 'mexican' lime (citrus aurantifolia swingle) was transformed with constructs that contained chimeric promoter-gus gene fusions of phloem-specific rolc promoter of agrobacterium rhizogenes, arabidopsis thaliana sucrose-h(+) symporter (atsuc2) gene promoter of arabidopsis thaliana, rice tungro bacilliform virus (rtbv) promoter and sucrose synthase l (rss1) gene promoter of oryza sativa (rice). histochemical β-glucuronidase (gus) analysis revealed vascular-specific expression of the gus protein in ... | 2012 | 22228816 |
| development of marker-free insect-resistant indica rice by agrobacterium tumefaciens-mediated co-transformation. | agrobacterium-mediated co-transformation is an efficient strategy to generate marker-free transgenic plants. in this study, the vectors pmf-2a(∗) containing a synthetic cry2a(∗) gene driven by maize ubiquitin promoter and pcambia1301 harboring hygromycin phosphotransferase gene (hpt) were introduced into minghui86 (oryza sativa l. ssp. indica), an elite indica restorer line. two independent transformants containing both the cry2a(∗) gene and hpt gene were regenerated. several homozygous marker-f ... | 2016 | 27833629 |
| agrobacterium-mediated genetic transformation of commercially elite rice restorer line using nptii gene as a plant selection marker. | transformation of commercially important indica cultivars remains challenging for the scientific community even though agrobacterium-mediated transformation protocols for a few indica rice lines have been well established. we report successful transformation of a commercially important restorer line jk1044r of indica rice hybrid jkrh 401. while following existing protocol, we optimized several parameters for callusing, regeneration and genetic transformation of jk1044r. calli generated from the ... | 2016 | 27186018 |
| production and characterization of recombinant human acid α-glucosidase in transgenic rice cell suspension culture. | pompe disease is a fatal genetic muscle disorder caused by a deficiency of acid α-glucosidase (gaa), a glycogen-degrading lysosomal enzyme. in this study, the human gaa cdna gene was synthesized from human placenta cells and cloned into a plant expression vector under the control of the rice α-amylase 3d (ramy3d) promoter. the plant expression vector was introduced into rice calli (oryza sativa l. cv. dongjin) mediated by agrobacterium tumefaciens. genomic dna pcr and northern blot analysis were ... | 2016 | 27050503 |
| multigene engineering in rice using high-capacity agrobacterium tumefaciens bibac vectors. | the high-capacity binary bacterial artificial chromosome (bibac) vector system permits the insertion of large fragments of dna, up to 150 kb, into plants via agrobacterium-mediated transformation. here, we describe an optimized protocol for transformation of japonica rice (oryza sativa l.) using this system. calli derived from mature embryos are transformed using agrobacterium strain lba4404 that carries the bibac vector and the super-virulent helper plasmid pch32. transformed calli are then reg ... | 2016 | 26614279 |
| 2-acetyl-1-pyrroline augmentation in scented indica rice (oryza sativa l.) varieties through δ(1)-pyrroline-5-carboxylate synthetase (p5cs) gene transformation. | 2-acetyl-1-pyrroline (2ap) has been identified as a principal aroma compound in scented rice varieties. δ(1)-pyrroline-5-carboxylate synthetase (p5cs) gene is reported to regulate the proline synthesis in plants and acts as the precursor of 2ap. two scented indica rice varieties, namely ambemohar 157 and indrayani, were subjected to agrobacterium tumefaciens-mediated genetic transformation containing p5cs gene. overexpression of p5cs led to a significant increase in proline, p5cs enzyme activity ... | 2015 | 26340891 |
| comparative whole-genome analyses of selection marker-free rice-based cholera toxin b-subunit vaccine lines and wild-type lines. | we have developed a rice-based oral cholera vaccine named mucorice-ctb (cholera toxin b-subunit) by using an agrobacterium tumefaciens-mediated co-transformation system. to assess the genome-wide effects of this system on the rice genome, we compared the genomes of three selection marker-free mucorice-ctb lines with those of two wild-type rice lines (oryza sativa l. cv. nipponbare). mutation profiles of the transgenic and wild-type genomes were examined by next-generation sequencing (ngs). | 2015 | 25653106 |
| efficient generation of marker-free transgenic rice plants using an improved transposon-mediated transgene reintegration strategy. | marker-free transgenic plants can be developed through transposon-mediated transgene reintegration, which allows intact transgene insertion with defined boundaries and requires only a few primary transformants. in this study, we improved the selection strategy and validated that the maize (zea mays) activator/dissociation (ds) transposable element can be routinely used to generate marker-free transgenic plants. a ds-based gene of interest was linked to green fluorescent protein in transfer dna ( ... | 2015 | 25371551 |
| rice, indica (oryza sativa l.). | indica varieties, which are generally recalcitrant to tissue culture and transformation, occupy 80 % of rice cultivation area in the world. therefore, transformation method for indica rice must be improved greatly so that global rice production would take full advantage of cutting-edge biotechnology. an efficient protocol for indica transformation mediated by agrobacterium tumefaciens is hereby described. immature embryos collected from plants in a greenhouse are cocultivated with a. tumefaciens ... | 2015 | 25300838 |
| enhanced resistance to blast fungus in rice (oryza sativa l.) by expressing the ribosome-inactivating protein α-momorcharin. | rice blast caused by magnaporthe grisea is one of the three major diseases that seriously affect the rice production. alpha-momorcharin (α-mc), a ribosome-inactivating protein (rip) isolated from momordica charantia seeds, has antifungal effects in vitro. in this study, the α-mc gene was constitutively expressed under the control of the 2×35s promoter in transgenic rice (oryza sativa l.) using an agrobacterium tumefaciens-mediated method. the nine transgenic plants were obtained and confirmed by ... | 2014 | 24467890 |
| identification and characterization of granule bound starch synthase i (gbssi) gene of tartary buckwheat (fagopyrum tataricum gaertn.). | tartary buckwheat (fagopyrum tataricum gaertn.) is increasingly considered as an important functional food material because of its rich nutraceutical compounds. reserve starch is the major component of tartary buckwheat seed. however, the gene sequences and the molecular mechanism of tartary buckwheat starch synthesis are unknown so far. in this study, the complete genomic sequence and full-size cdna coding tartary buckwheat granule-bound starch synthase i (ftgbssi), which is responsible for amy ... | 2014 | 24211386 |
| genetic transformation of major cereal crops. | of the more than 50,000 edible plant species in the world, at least 10,000 species are cereal grains. three major cereal crops, rice (oryza sativa), maize (zea mays), and wheat (triticum sp.), provide two-thirds of the world's food energy intake. although crop yields have improved tremendously thanks to technological advances in the past 50 years, population increases and climate changes continue to threaten the sustainability of current crop productions. whereas conventional and marker-assisted ... | 2013 | 24166432 |
| the transformation of the photo-thermo sensitive genic male-sterile line 261s of rice via an expression vector containing the anti-waxy gene. | transgenic photo-thermo sensitive genic male sterility oryza sativa l. cv. "261s" plants with the anti-waxy gene were successfully obtained using an agrobacterium tumefaciens-mediated co-transformation method. marker-free homozygous transgenic lines with the anti-waxy gene were obtained. the setting seed rates of the transgenic plants via self-pollination or via crossing with the restorer line wx99075 rice and the 1000-grain weight of the transgenic plants and the f2 hybrid seeds obtained by cro ... | 2013 | 23853508 |
| an improved agrobacterium-mediated transformation of recalcitrant indica rice (oryza sativa l.) cultivars. | agrobacterium-mediated transformation of indica rice varieties has been quite difficult as these are recalcitrant to in vitro responses. in the present study, we established a high-efficiency agrobacterium tumefaciens-mediated transformation system of rice (oryza sativa l. ssp. indica) cv. ir-64, lalat, and iet-4786. agrobacterium strain eha-101 harboring binary vector pig121-hm, containing a gene encoding for β-glucuronidase (gus) and hygromycin resistance, was used in the transformation experi ... | 2013 | 22878598 |
| metabolic factors capable of inducing agrobacterium vir gene expression are present in rice (oryza sativa l.). | the effects of exudates and extracts from suspension cultures or various parts of rice (oryza sativa l.) plants on induction of vir (virulence) gene expression in agrobacterium tumefaciens were examined. only leaf extracts from panicle-differentiating plants to flowering plants were able to strongly induce activation and expression of vir genes. this induction was similar to that observed with 2 μm acetosyringone (as), yet there was no synergy between as and rice extracts. responses to vir-induc ... | 1993 | 24196854 |
| rhizobium oryziradicis sp. nov., isolated from the root of rice. | two gram-negative, aerobic, rod-shaped endophytic bacterial strains, n19t and n11-2, were isolated from fresh rice (oryza sativa) root during investigating the rice endophytic bacterial diversity. the 16s rrna gene sequence results indicated that the similarity between strains n19t and n11-2 was 100 %. both of them phylogenetically belong to the genus rhizobium, with close similarity to rhizobium taibaishanense ccnwsx 0483t (97.7 %), followed by rhizobium vitis ncppb 3554t (97.5 %). the sequence ... | 2016 | 27959784 |
| a phage display-selected peptide inhibitor of agrobacterium vitis polygalacturonase. | agrobacterium vitis, the causal agent of crown gall of grapevine, is a threat to viticulture worldwide. a major virulence factor of this pathogen is polygalacturonase, an enzyme that degrades pectin components of the xylem cell wall. a single gene encodes for the polygalacturonase gene. disruption of the polygalacturonase gene results in a mutant that is less pathogenic and produces significantly fewer root lesions on grapevines. thus, the identification of peptides or proteins that could inhibi ... | 2016 | 26177065 |
| genetic analysis and qtl detection for resistance to white tip disease in rice. | the inheritance of resistance to white tip disease (wtdr) in rice (oryza sativa l.) was analyzed with an artificial inoculation test in a segregating population derived from the cross between tetep, a highly resistant variety that was identified in a previous study, and a susceptible cultivar. three resistance-associated traits, including the number of aphelenchoides besseyi (a. besseyi) individuals in 100 grains (na), the loss rate of panicle weight (lrpw) and the loss rate of the total grains ... | 2014 | 25162680 |
| efficacy of oryza sativa husk and quercus phillyraeoides extracts for the in vitro and in vivo control of fungal rot disease of white yam (dioscorea rotundata poir). | tuber rot disease is a major constraint to white yam (dioscorea rotundata) production, accounting for 50-60% of annual yield losses in nigeria. the main method of control using synthetic fungicides is being discouraged due to human and environmental health hazards. the potential of oryza sativa husk (osh) and quercus phillyraeoides (qp) extracts for the in vitro and in vivo control of six virulent rot-causing fungal pathogens, lasiodiplodia theobromae, aspergillus niger, rhizoctonia solani, peni ... | 2014 | 25674452 |
| isolation and evaluation of native cellulose degrading microorganisms for efficient bioconversion of weed biomass and rice straw. | cellulose decomposing microorganisms (cdms) are important for efficient bioconversion of plant biomasses. to this end, we isolated seven fungal isolates (aspergillus wentii, fusarium solani, mucor sp., penicillum sp., trichoderma harzaianum, trichoderma sp.1 and trichoderma sp.2) and three bacterial isolates (bacterial isolate i, ii and iii) from partially decomposed farm yard manure, rice straw and vermicompost, and evaluated them for decomposition of rice straw (oryza sativa), ipomoea camea an ... | 2014 | 25004759 |
| activation of a calcium-dependent protein kinase involved in the azospirillum growth promotion in rice. | rice seedlings (oryza sativa) inoculated with the plant growth-promoting rhizobacteria azospirillum brasilense ft326 showed an enhanced development of the root system 3 days after inoculation. later on, a remarkable enlargement of shoots was also evident. an increase in the ca(2+)-dependent histone kinase activity was also detected as a result of inoculation. the biochemical characterization and western-blot analysis of the kinase strongly supports the hypothesis that it belongs to a member of t ... | 2017 | 28044270 |
| differential responses of oryza sativa secondary metabolism to biotic interactions with cooperative, commensal and phytopathogenic bacteria. | profiling of plant secondary metabolite allows to differentiate the different types of ecological interactions established between rice and bacteria. rice responds to ecologically distinct bacteria by altering its content of flavonoids and hydroxycinnamic acid derivatives. plants' growth and physiology are strongly influenced by the biotic interactions that plants establish with soil bacterial populations. plants are able to sense and to respond accordingly to ecologically distinct bacteria, by ... | 2015 | 26303982 |
| genome wide profiling of azospirillum lipoferum 4b gene expression during interaction with rice roots. | azospirillum-plant cooperation has been mainly studied from an agronomic point of view leading to a wide description of mechanisms implicated in plant growth-promoting effects. however, little is known about genetic determinants implicated in bacterial adaptation to the host plant during the transition from free-living to root-associated lifestyles. this study aims at characterizing global gene expression of azospirillum lipoferum 4b following a 7-day-old interaction with two cultivars of oryza ... | 2014 | 24283406 |
| seed-borne endophytic bacillus amyloliquefaciens rwl-1 produces gibberellins and regulates endogenous phytohormones of oryza sativa. | some microorganisms are adapted to an endophytic mode, living symbiotically with plants through vertical transmission in seeds. the role of plant growth-promoting endophytes has been well studied, but those of seed-associated endophytic bacteria are less understood. the current study aimed to isolate and identify bacterial endophytes associated with rice (oryza sativa l. 'jin so mi') seeds, their potential to produce gibberellins (gas), and role in improving host-plant physiology. the isolated b ... | 2016 | 27182958 |
| a consortium of rhizobacterial strains and biochemical growth elicitors improve cold and drought stress tolerance in rice (oryza sativa l.). | in the present study, a consortium of two rhizobacteria bacillus amyloliquefaciens bk7 and brevibacillus laterosporus b4, termed 'bb', biochemical elicitors salicylic acid and β-aminobutyric acid (sb) and their mixture (bbsb) were investigated for cold and drought stress tolerance in rice plants. after withholding water for 16 days, rice plants treated with bbsb showed 100% survival, improved seedling height (35.4 cm), shoot number (6.12), and showed minimum symptoms of chlorosis (19%), wilting ... | 2016 | 26681628 |
| effect of silver nanoparticles on oryza sativa l. and its rhizosphere bacteria. | silver nanoparticles (agnps) are widely used as antibacterial and antifungal agents in agriculture. nevertheless, these nanoparticles with newborn properties pose a potential risk to the environment, due to contact with crops and bacteria that are beneficial to the soil. this study is based on the examination of the phytotoxic effects of agnps on oryza sativa l. and some of its rhizosphere bacteria, by physiological and biochemical assays. in order to study the complex interaction of the agnps l ... | 2013 | 23174269 |
| antimicrobial compounds from endophytic streptomyces sp. bcc72023 isolated from rice (oryza sativa l.). | an endophytic actinomycete strain bcc72023 was isolated from rice (oryza sativa l.) and identified as the genus streptomyces, based on phenotypic, chemotaxonomic and 16s rrna gene sequence analyses. the strain showed 99.80% similarity compared with streptomyces samsunensis m1463(t). chemical investigation led to the isolation of three macrolides, efomycins m (1), g (2) and oxohygrolidin (3), along with two polyethers, abierixin (4) and 29-o-methylabierixin (5). to our knowledge, this is the firs ... | 2016 | 26809052 |
| root associated bacillus sp. improves growth, yield and zinc translocation for basmati rice (oryza sativa) varieties. | plant associated rhizobacteria prevailing in different agro-ecosystems exhibit multiple traits which could be utilized in various aspect of sustainable agriculture. two hundred thirty four isolates were obtained from the roots of basmati-385 and basmati super rice varieties growing in clay loam and saline soil at different locations of punjab (pakistan). out of 234 isolates, 27 were able to solubilize zinc (zn) from different zn ores like zinc phosphate [zn3 (po4)2], zinc carbonate (znco3) and z ... | 2015 | 26635754 |
| arsenite-oxidizing bacteria exhibiting plant growth promoting traits isolated from the rhizosphere of oryza sativa l.: implications for mitigation of arsenic contamination in paddies. | arsenite-oxidizing bacteria exhibiting plant growth promoting (pgp) traits can have the advantages of reducing as-uptake by rice and promoting plant growth in as-stressed soil. a gram-positive bacterium bacillus flexus aso-6 resistant to high levels of as (32 and 280 mm for arsenite and arsenate, respectively) and exhibiting elevated rates of as(iii) oxidation (vmax=1.34 μm min(-1) 10(-7) cell) was isolated from rhizosphere of rice. the presence of aoxb gene and exhibition of as(iii)-oxidase enz ... | 2016 | 26448489 |
| cd immobilization and reduced tissue cd accumulation of rice (oryza sativa wuyun-23) in the presence of heavy metal-resistant bacteria. | two metal-resistant bacillus megaterium h3 and neorhizobium huautlense t1-17 were investigated for their immobilization of cd in solution and tissue cd accumulation of rice (oryza sativa wuyun-23) in the cd-contaminated soil. strains h3 and t1-17 decreased 79-96% of water-soluble cd in solution and increased grain biomass in the high cd-contaminated soil. inoculation with h3 and t1-17 significantly decreased the root (ranging from 25% to 58%), above-ground tissue (ranging from 13% to 34%), and p ... | 2017 | 28011421 |
| diversity in guanosine 3',5'-bisdiphosphate (ppgpp) sensitivity among guanylate kinases of bacteria and plants. | the guanosine 3',5'-bisdiphosphate (ppgpp) signaling system is shared by bacteria and plant chloroplasts, but its role in plants has remained unclear. here we show that guanylate kinase (gk), a key enzyme in guanine nucleotide biosynthesis that catalyzes the conversion of gmp to gdp, is a target of regulation by ppgpp in chloroplasts of rice, pea, and arabidopsis. plants have two distinct types of gk that are localized to organelles (gkpm) or to the cytosol (gkc), with both enzymes being essenti ... | 2014 | 24722991 |
| pyramiding and evaluation of both a foreign bacillus thuringiensis and a lysine-rich protein gene in the elite indica rice 9311. | gene pyramiding is an efficient approach for the genetic improvement of multiple agronomic traits simultaneously. in this study, we pyramided two foreign genes, cry1ac driven by the rice actin i promoter, and lysine-rich protein (lrp), driven by the endosperm-specific glutelin1 (gt1) promoter, into the elite indica cultivar 9311. these two genes were chosen in an attempt to enhance insect-resistance and lysine (lys) content. in the pyramided line, the foreign gene cry1ac was efficiently expresse ... | 2016 | 27795684 |
| marker-free transgenic rice expressing the vegetative insecticidal protein (vip) of bacillus thuringiensis shows broad insecticidal properties. | genetically engineered rice lines with broad insecticidal properties against major lepidopteran pests were generated using a synthetic, truncated form of vegetative insecticidal protein (syn vip3br) from bacillus thuringiensis. the selectable marker gene and the redundant transgene(s) were eliminated through cre/ lox mediated recombination and genetic segregation to make consumer friendly bt -rice. for sustainable resistance against lepidopteran insect pests, chloroplast targeted synthetic versi ... | 2016 | 27165311 |
| transgenic rice expressing the cry2ax1 gene confers resistance to multiple lepidopteran pests. | a chimeric bacillus thuringiensis toxin (bt) gene, cry2ax1was cloned in a bi-selectable marker free binary vector construct. the cry2ax1 gene, driven by the chrysanthemum rbcs1 promoter, was introduced into jk1044r, the restorer line (oryza sativa l. ssp. indica) of a notified commercially grown rice hybrid in india, by agrobacterium-mediated transformation. its effect against two major lepidopteran insect pests viz., yellow stem borer (ysb) scirpophaga incertulas, rice leaf folder (rlf) cnaphal ... | 2016 | 27017220 |
| the efficacy of bacillus thuringiensis spp. galleriae against rice water weevil (coleoptera: curculionidae) for integrated pest management in california rice. | rice water weevil (lissorhoptrus oryzophilus kushel) is the most damaging insect pest of rice in the united states. larval feeding on the roots stunt growth and reduce yield. current pest management against the weevil in california relies heavily on pyrethroids that can be damaging to aquatic food webs. examination of an environmentally friendly alternative biopesticide based on bacillus thuringiensis spp. galleriae chemistry against rice water weevil larvae showed moderate levels of activity in ... | 2015 | 26470102 |
| development of photoperiod- and thermo-sensitive male sterility rice expressing transgene bacillus thuringiensis. | stem borers and leaffolders are the main pests that cause severe damage in rice (oryza sativa l.) production worldwide. we developed the first photoperiod- and thermo-sensitive male sterility (ptsms) rice 208s with the cry1ab/1ac bacillus thuringiensis (bt) gene, through sexual crossing with huahui 1 (elite line with the cry1ab/1ac gene). the novel 208s and its hybrids presented high and stable resistance to stem borers and leaffolders, and the content of cry1ab/1ac protein in chlorophyllous tis ... | 2015 | 26366116 |
| nondestructive determination of transgenic bacillus thuringiensis rice seeds (oryza sativa l.) using multispectral imaging and chemometric methods. | crop-to-crop transgene flow may affect the seed purity of non-transgenic rice varieties, resulting in unwanted biosafety consequences. the feasibility of a rapid and nondestructive determination of transgenic rice seeds from its non-transgenic counterparts was examined by using multispectral imaging system combined with chemometric data analysis. principal component analysis (pca), partial least squares discriminant analysis (plsda), least squares-support vector machines (ls-svm), and pca-back p ... | 2014 | 24491704 |
| proteomics study of silver nanoparticles toxicity on bacillus thuringiensis. | emerging technologies in functional genomics and proteomics provide a way of achieving high-throughput analyses, understanding effects on protein populations and sub-populations and follow up environmental stresses. to accomplish these, the action of homemade spherical silver nanoparticles colloidal suspension (agnps) against bacillus thuringiensis (isolate from oryza sativa l. rhizosphere) was investigated by a proteomic approach (2-de and nanolc/ft-icr ms identification). thirty four responsiv ... | 2014 | 24290895 |
| inducible expression of a fusion gene encoding two proteinase inhibitors leads to insect and pathogen resistance in transgenic rice. | plant proteinase inhibitors (pis) are considered as candidates for increased insect resistance in transgenic plants. insect adaptation to pi ingestion might, however, compromise the benefits received by transgenic expression of pis. in this study, the maize proteinase inhibitor (mpi), an inhibitor of insect serine proteinases, and the potato carboxypeptidase inhibitor (pci) were fused into a single open reading frame and introduced into rice plants. the two pis were linked using either the proce ... | 2014 | 24237606 |
| evaluation of real-time pcr detection methods for detecting rice products contaminated by rice genetically modified with a cpti-kdel-t-nos transgenic construct. | genetically modified (gm) rice (oryza sativa) lines, such as insecticidal kefeng and kemingdao, have been developed and found unauthorised in processed rice products in many countries. therefore, qualitative detection methods for the gm rice are required for the gm food regulation. a transgenic construct for expressing cowpea (vigna unguiculata) trypsin inhibitor (cpti) was detected in some imported processed rice products contaminated with kemingdao. the 3' terminal sequence of the identified t ... | 2013 | 23871003 |
| alteration of leaf metabolism in bt-transgenic rice (oryza sativa l.) and its wild type under insecticide stress. | insecticide is always used to control the damage from pests, while the potential influence on plants is rarely known. time-course metabolic changes of wild and bacillus thuringiensis (bt) transgenic rice (oryza sativa l.) plants after insecticide treatment were investigated by using gas chromatography-mass spectrometry (gc-ms). a combined statistical strategy of 2-way anova and multivariate analyses (principal component analysis and hierarchal cluster analysis) was performed to find the stress-a ... | 2012 | 22768924 |
| complete genome sequence of bacillus velezensis cbmb205, a phosphate-solubilizing bacterium isolated from the rhizoplane of rice in the republic of korea. | bacillus velezensis cbmb205 (= kacc 13105(t) = nccb 100236(t)) was isolated from the rhizoplane of rice (oryza sativa l. cv. o-dae). according to previous studies, this bacterium has several genes that can promote plant growth, such as the phosphorus-solubilizing protein-coding gene. here, we present the first complete genome of b. velezensis cbmb205. | 2016 | 27417835 |
| bacillus velezensis is not a later heterotypic synonym of bacillus amyloliquefaciens; bacillus methylotrophicus, bacillus amyloliquefaciens subsp plantarum and 'bacillus oryzicola' are later heterotypic synonyms of bacillus velezensis based on phylogenomics. | bacillus velezensis was previously reported to be a later heterotypic synonym of bacillus amyloliquefaciens, based on dna-dna relatedness values. we have sequenced a draft genome of b. velezensis nrrl b-41580t. comparative genomics and dna-dna relatedness calculations show that it is not a synonym of b. amyloliquefaciens. it was instead synonymous with bacillus methylotrophicus. 'bacillus oryzicola' is a recently described species that was isolated as an endophyte of rice (oryza sativa). the str ... | 2015 | 26702995 |
| increased expression of fe-chelatase leads to increased metabolic flux into heme and confers protection against photodynamically induced oxidative stress. | fe-chelatase (fech, ec 4.99.1.1) inserts fe(2+) into protoporphyrin ix (proto ix) to form heme, which influences the flux through the tetrapyrrole biosynthetic pathway as well as fundamental cellular processes. in transgenic rice (oryza sativa), the ectopic expression of bradyrhizobium japonicum fech protein in cytosol results in a substantial increase of fech activity compared to wild-type (wt) rice and an increasing level of heme. interestingly, the transgenic rice plants showed resistance to ... | 2014 | 25037078 |
| brevundimonas diminuta mediated alleviation of arsenic toxicity and plant growth promotion in oryza sativa l. | arsenic (as), a toxic metalloid adversely affects plant growth in polluted areas. in the present study, we investigated the possibility of improving phytostablization of arsenic through application of new isolated strain brevundimonas diminuta (nbri012) in rice plant [oryza sativa (l.) var. sarju 52] at two different concentrations [10ppm (low toxic) and 50ppm (high toxic)] of as. the plant growth promoting traits of bacterial strains revealed the inherent ability of siderophores, phosphate solu ... | 2016 | 26650422 |
| brome mosaic virus infection of rice results in decreased accumulation of rna1. | brome mosaic virus (bmv) (the russian strain) infects monocot plants and has been studied extensively in barley and wheat. here, we report bmv can systemically infect rice (oryza sativa var. japonica), including cultivars in which the genomes have been determined. the bmv capsid protein can be found throughout the inoculated plants. however, infection in rice exhibits delayed symptom expression or no symptoms when compared with wheat (triticum aestivum). the sequences of bmv rnas isolated from r ... | 2015 | 26024443 |
| improved nutrient use efficiency increases plant growth of rice with the use of iaa-overproducing strains of endophytic burkholderia cepacia strain rre25. | effect of indole acetic acid (iaa)-overproducing mutants of burkholderia cepacia (rre25), a member of β-subclass of proteobacteria and naturally occurring rice endophyte, was observed on the growth of rice (oryza sativa l.) plants grown under greenhouse conditions. nine mutants were characterized for altered biosynthesis of iaa after nitrous acid mutagenesis. these mutants were grouped into two classes: class i mutants have reduced production of iaa as compared to the wild type, while class ii m ... | 2013 | 23615705 |
| direct detection of the plant pathogens burkholderia glumae, burkholderia gladioli pv. gladioli, and erwinia chrysanthemi pv. zeae in infected rice seedlings using matrix assisted laser desorption/ionization time-of-flight mass spectrometry. | the plant pathogens burkholderia glumae, burkholderia gladioli pv. gladioli, and erwinia chrysanthemi pv. zeae were directly detected in extracts from infected rice seedlings by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms). this method did not require culturing of the pathogens on artificial medium. in the maldi-tof ms analysis, peaks originating from bacteria were found in extracts from infected rice seedlings. the spectral peaks showed significant ... | 2016 | 26319185 |
| effects of colonization of the roots of domestic rice (oryza sativa l. cv. amaroo) by burkholderia pseudomallei. | burkholderia pseudomallei is a saprophytic bacterium that causes melioidosis and is often isolated from rice fields in southeast asia, where the infection incidence is high among rice field workers. the aim of this study was to investigate the relationship between this bacterium and rice through growth experiments where the effect of colonization of domestic rice (oryza sativa l. cv amaroo) roots by b. pseudomallei could be observed. when b. pseudomallei was exposed to surface-sterilized seeds, ... | 2015 | 25911477 |
| effect of alanine, leucine, and arginine substitution on antimicrobial activity against candida albicans and action mechanism of a cationic octadecapeptide derived from α-amylase of rice. | amyi-1-18, an antimicrobial peptide, is a cationic α-helical octadecapeptide derived from α-amylase of rice (oryza sativa l. japonica) that contains four cationic amino acid residues (two arginines and two lysines). to enhance the antifungal activity of amyi-1-18 against candida albicans, 11 analogs bearing substitutions with alanine, leucine, and/or arginine, which were designed on the basis of the helical wheel projection of amyi-1-18, were synthesized, and their antifungal activity was invest ... | 2016 | 26850838 |
| antimicrobial activity and mechanism of action of a novel cationic α-helical octadecapeptide derived from α-amylase of rice. | amyi-1-18, an octadecapeptide derived from α-amylase (amyi-1) of rice (oryza sativa l. japonica), is a novel cationic α-helical antimicrobial peptide (amp) that contains two lysine and two arginine residues. the antimicrobial activity of amyi-1-18 against human pathogens was quantitatively evaluated using a chemiluminescence method that measures atp derived from viable cells. of the ten kinds of human pathogens, amyi-1-18 exhibited antimicrobial activity against nine. its 50% growth-inhibitory c ... | 2015 | 25581614 |
| antimicrobial activity and mechanism of action of a novel cationic α-helical octadecapeptide derived from heat shock protein 70 of rice. | hsp70(241-258), an octadecapeptide derived from the heat shock protein 70 (hsp70) of rice (oryza sativa l. japonica), is a novel cationic α-helical antimicrobial peptide (amp) that contains four lysine, two arginine, and two histidine residues. the antimicrobial activity of hsp70(241-258) against porphyromonas gingivalis, a periodontal pathogen, and candida albicans, an opportunistic fungal pathogen, was quantitatively evaluated using a chemiluminescence method that measures atp derived from via ... | 2013 | 23973864 |
| function of xyloglucan endotransglucosylase/hydrolases in rice. | although xyloglucans are ubiquitous in land plants, they are less abundant in poales species than in eudicotyledons. poales cell walls contain higher levels of β-1,3/1,4 mixed-linked glucans and arabinoxylans than xyloglucans. despite the relatively low level of xyloglucans in poales, the xyloglucan endotransglucosylase/hydrolase (xth) gene family in rice (oryza sativa) is comparable in size to that of the eudicotyledon arabidopsis thaliana. this raises the question of whether xyloglucan is a su ... | 2014 | 24363334 |
| constitutive expression of cell wall invertase genes increases grain yield and starch content in maize. | grain size, number and starch content are important determinants of grain yield and quality. one of the most important biological processes that determine these components is the carbon partitioning during the early grain filling, which requires the function of cell wall invertase. here, we showed the constitutive expression of cell wall invertase-encoding gene from arabidopsis, rice (oryza sativa) or maize (zea mays), driven by the cauliflower mosaic virus (camv) 35s promoter, all increased cel ... | 2013 | 23926950 |
| ossuv3 dual helicase functions in salinity stress tolerance by maintaining photosynthesis and antioxidant machinery in rice (oryza sativa l. cv. ir64). | to overcome the salinity-induced loss of crop yield, a salinity-tolerant trait is required. the suv3 helicase is involved in the regulation of rna surveillance and turnover in mitochondria, but the helicase activity of plant suv3 and its role in abiotic stress tolerance have not been reported so far. here we report that the oryza sativa (rice) suv3 protein exhibits dna and rna helicase, and atpase activities. furthermore, we report that suv3 is induced in rice seedlings in response to high level ... | 2013 | 23808500 |
| production of green fluorescent protein in transgenic rice seeds. | immature embryos from immature seeds of rice (oryza sativa l.) were transformed by biolistic bombardment with the plasmid carrying the coding region of the hygromycin phosphotransferase gene under the control of the 5' region of the cauliflower mosaic virus 35s promoter and the synthetic green fluorescence protein gene (sgfp) under the control of the maize ubiquitine promoter. southern blot analysis confirmed the stable integration of hpt and sgfp genes in transformants. subsequently leaves from ... | 2013 | 23755644 |
| a desd-box helicase functions in salinity stress tolerance by improving photosynthesis and antioxidant machinery in rice (oryza sativa l. cv. pb1). | the exact mechanism of helicase-mediated salinity tolerance is not yet understood. we have isolated a desd-box containing cdna from pisum sativum (pea) and named it as pdh45. it is a unique member of dead-box helicase family; containing desd instead of dead/h. pdh45 overexpression driven by constitutive cauliflower mosaic virus-35s promoter in rice transgenic [oryza sativa l. cv. pusa basmati 1 (pb1)] plants confers salinity tolerance by improving the photosynthesis and antioxidant machinery. th ... | 2013 | 23456247 |
| regeneration of transgenic rice with bacterial ipt gene driven by senescence specific (sag12) promoter by particle bombardment. | transgenic rice plants were generated using particle bombardment to introduce the agrobacterium cytokinin biosynthesis gene driven by arabidopsis (arabidopsis thaliana) senescence specific promoter (sag12) for delaying leaf senescence. using two plasmids we co-transformed one week old embryogenic calli derived from mature japonica rice (oryza sativa) variety chin guang. the selectable marker hygromycin phosphotransferase (hph) gene and the reporter gene b-ß-glucuronidase (uida), both under the c ... | 2012 | 24575232 |
| degradation of transgene dna in genetically modified herbicide-tolerant rice during food processing. | in order to assess the effect of food processing on the degradation of exogenous dna components in sweet rice wine and rice crackers made from genetically modified (gm) rice (oryza sativa l.), we developed genomic dna extraction methods and compared the effect of different food processing procedures on dna degradation. it was found that the purity, quantity and quality of dna by alkaline lysis method were higher than by ctab (cetyltrimethylammonium bromide) method. for sweet rice wine, camv35s ( ... | 2011 | 21871942 |
| overexpression of the oszip4 zinc transporter confers disarrangement of zinc distribution in rice plants. | zinc (zn), an essential nutrient in cells, plays a vital role in controlling cellular processes such as growth, development, and differentiation. although the mechanisms of zn translocation in rice plants (oryza sativa) are not fully understood, it has recently received increased interest. oszip4 is a zn transporter that localizes to apical cells. transgenic rice plants overexpressing the oszip4 gene under the control of the cauliflower mosaic virus (camv) 35s promoter were produced. the zn conc ... | 2007 | 17630290 |
| activation of the oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, arr1. | using in silico methods, several putative phytohormone-responsive cis-elements in the oryza sativa non-symbiotic haemoglobin (nshb) 1-4 and arabidopsis thaliana nshb1-2 promoters have been identified. an osnshb2 promoter::gus reporter gene fusion shows tissue-specific expression in a. thaliana. gus expression was observed in roots, the vasculature of young leaves, in flowers, and in the pedicel/stem junction. in transient assays, activity of the osnshb2 promoter was significantly up-regulated in ... | 2004 | 15258171 |
| structure, expression and promoter activity of two polyubiquitin genes from rice (oryza sativa l.). | we have isolated two rice polyubiquitin genes designated as rubq1 and rubq2 by screening a bacterial artificial chromosome (bac) genomic library with a 32p-labeled ubiquitin cdna probe. dna sequence data revealed that both genes contained an open reading frame encoding a hexameric precursor ubiquitin and an intron immediate upstream of the initiation codon. the deduced amino acid sequences of both genes were identical to each other and to other plant ubiquitin sequences. several putative regulat ... | 2000 | 10936527 |
| the rab16b promoter of rice contains two distinct abscisic acid-responsive elements. | to localize abscisic acid (aba)-inducible gene expression of rab16 genes, rab16a promoter was linked to the gusa reporter gene encoding beta-glucuronidase and introduced into rice (oryza sativa l.) plants. the activity of rab16a promoter was induced by aba and osmotic stresses in various tissues of vegetative and floral organs. in anthers and embryos, rab16a promoter was active in the absence of aba. to elucidate cis-elements of the rab16 promoter that confer aba-inducible expression, variously ... | 1996 | 8883374 |
| transgenic indica rice (oryza sativa l.) plants obtained by direct gene transfer to protoplasts. | we have established a reproducible procedure for transformation of protoplasts and regeneration of transgenic plants for an improved indica rice cultivar ir43. mature embryo-derived calli were placed in liquid culture medium containing maltose to establish meristematically active, embryogenic cell suspension lines. in order to obtain transgenic plants, a chimeric hygromycin phosphotransferase hph gene under the control of the cauliflower mosaic virus camv 35s promoter was introduced into protopl ... | 1994 | 7764447 |
| expression of a rice homeobox gene causes altered morphology of transgenic plants. | we have isolated a cdna clone encoding a homeobox sequence from rice. dna sequence analysis of this clone, which was designated as oryza sativa homeobox 1 (osh1), and a genomic clone encoding the osh1 sequence have shown that the osh1 gene consists of five exons and encodes a polypeptide of 361 amino acid residues. restriction fragment length polymorphism analysis has shown that osh1 is a single-copy gene located near the phytochrome gene on chromosome 3. introduction of the cloned osh1 gene int ... | 1993 | 8104574 |
| regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. | suspension cells of oryza sativa l. (rice) were transformed, by microprojectile bombardment, with plasmids carrying the coding region of the streptomyces hygroscopicus phosphinothricin acetyl transferase (pat) gene (bar) under the control of either the 5' region of the rice actin 1 gene (act1) or the cauliflower mosaic virus (camv) 35s promoter. subsequently regenerated plants display detectable pat activity and are resistant to basta(tm), a phosphinothricin (ppt)-based herbicide. dna gel blot a ... | 1992 | 24213292 |
| a sulfonylurea herbicide resistance gene from arabidopsis thaliana as a new selectable marker for production of fertile transgenic rice plants. | a mutant acetolactate synthase (als) gene, csr1-1, isolated from sulfonylurea herbicide-resistant arabidopsis thaliana, was placed under control of a cauliflower mosaic virus 35s promoter (35s). rice protoplasts were transformed with the 35s/als chimeric gene and regenerated into fertile transgenic rice (oryza sativa) plants. the 35s/als gene was expressed effectively as demonstrated by northern blot hybridization analysis, and conferred to transformed calli at least 200-fold greater chlorsulfur ... | 1992 | 16653044 |
| characterization of cadmium-resistant bacteria and their potential for reducing accumulation of cadmium in rice grains. | cadmium (cd) pollution is a serious widespread environmental problem that not only destroys the microbial ecology of soil and decreases crop production, but also poses a serious risk to human health. many methods have been used for the remediation of cd pollution but none of these is totally satisfactory. microbial remediation strategies have attracted increasing interest since they are environmentally friendly and cost-effective. in the present study, three cd-resistant bacteria were isolated a ... | 2016 | 27341110 |
| oral immunisation of mice with transgenic rice calli expressing cholera toxin b subunit fused to consensus dengue cediii antigen induces antibodies to all four dengue serotypes. | dengue virus (denv) infection is an emerging global health threat. denv consists of four distinct serotypes, necessitating a tetravalent vaccine. in this study, expression of consensus envelope protein domain iii (cediii) fused to cholera toxin b subunit (ctb) in transgenic rice calli was improved using the luminal binding protein bip at the n-terminus and the sekdel signal sequences at the c-terminus, targeting the recombinant protein to endoplasmic reticulum (er). we found that the fusion prot ... | 2016 | 27566485 |
| dengue virus e glycoprotein production in transgenic rice callus. | dengue is a disease caused by dengue virus and represents the most important arthropod-borne viral disease in humans. dengue virus enters host cells via binding of envelope glycoprotein (e) to a receptor. in this study, plant expression vectors containing native and synthetic glycoprotein e genes (se) modified based on plant-optimized codon usage and fused with an er retention signal were constructed under control of the rice amylase 3d promoter expression system. plant expression vectors were i ... | 2014 | 25069989 |
| functional characterization of heat-shock protein 90 from oryza sativa and crystal structure of its n-terminal domain. | heat-shock protein 90 (hsp90) is an atp-dependent molecular chaperone that is essential for the normal functioning of eukaryotic cells. it plays crucial roles in cell signalling, cell-cycle control and in maintaining proteome integrity and protein homeostasis. in plants, hsp90s are required for normal plant growth and development. hsp90s are observed to be upregulated in response to various abiotic and biotic stresses and are also involved in immune responses in plants. although there are severa ... | 2015 | 26057797 |
| proteome compression via protein domain compositions. | in this paper, we study domain compositions of proteins via compression of whole proteins in an organism for the sake of obtaining the entropy that the individual contains. we suppose that a protein is a multiset of domains. since gene duplication and fusion have occurred through evolutionary processes, the same domains and the same compositions of domains appear in multiple proteins, which enables us to compress a proteome by using references to proteins for duplicated and fused proteins. such ... | 2014 | 24486717 |
| an activity-staining method on filtration paper enables high-throughput screening of temperature-sensitive and inactive mutations of rice α-amylase for improvement of rice grain quality. | α-amylase is a starch-hydrolyzing enzyme (ec 3.2.1.1) indispensable for germination of cereal seeds, but it is also expressed during the ripening stage. previous studies demonstrated that the enzyme is activated in developing rice seeds under extremely hot weather and triggers a loss of grain quality by hindering the accumulation of storage starch in the endosperm. since inactive or, preferably, heat-labile α-amylases are preferable for breeding premium rice, we developed a method for rapid scre ... | 2017 | 28339688 |
| development of an inducible male-sterility system in rice through pollen-specific expression of l-ornithinase (arge) gene of e. coli. | in the present investigation, an inducible male-sterility system has been developed in the rice. in order to introduce inducible male-sterility, the coding region of l-ornithinase (arge) gene of e. coli was fused to the oryza sativa indica pollen allergen (osipa) promoter sequence which is known to function specifically in the pollen grains. transgenic plants were obtained with arge gene and the transgenic status of plants was confirmed by pcr and southern blot analyses. rt-pcr analysis confirme ... | 2017 | 28167027 |
| the oshapl1-dth8-hd1 complex functions as the transcription regulator to repress heading date in rice. | heading date is an important agronomic trait related to crop yield. many genes related to heading date have already been identified in rice (oryza sativa), and a complicated, preliminary regulatory genetic network has also already been established, but the protein regulatory network is poorly understood. we have identified a novel heading date regulator, heme activator protein like 1 (oshapl1), which inhibits flowering under long-day conditions. oshapl1 is a nuclear-localized protein that is hig ... | 2017 | 28043949 |