| reactions upstream of glycerate-1,3-bisphosphate drive corynebacterium glutamicum (d)-lactate productivity under oxygen deprivation. | we previously demonstrated the simplicity of oxygen-deprived corynebacterium glutamicum to produce d-lactate, a primary building block of next-generation biodegradable plastics, at very high optical purity by introducing heterologous d-ldha gene from lactobacillus delbrueckii. here, we independently evaluated the effects of overexpressing each of genes encoding the ten glycolytic enzymes on d-lactate production in c. glutamicum. we consequently show that while the reactions catalyzed by glucokin ... | 2013 | 23712891 |
| two-step production of gamma-aminobutyric acid from cassava powder using corynebacterium glutamicum and lactobacillus plantarum. | production of gamma-aminobutyric acid (gaba) from crop biomass such as cassava in high concentration is desirable, but difficult to achieve. a safe biotechnological route was investigated to produce gaba from cassava powder by c. glutamicum g01 and l. plantarum gb01-21. liquefied cassava powder was first transformed to glutamic acid by simultaneous saccharification and fermentation with c. glutamicum g01, followed by biotransformation of glutamic acid to gaba with resting cells of l. plantarum g ... | 2015 | 26115763 |
| 3-methyl-1-butanol biosynthesis in an engineered corynebacterium glutamicum. | biofuel offers a promising solution to the adverse environmental problems and depletion in reserves of fossil fuels. higher alcohols including 3-methyl-1-butanol were paid much more attention as fuel substitute in recent years, due to its similar properties to gasoline. in the present work, 3-methyl-1-butanol production in engineered corynebacterium glutamicum was studied. α-ketoisovalerate decarboxylase gene (kivd) from lactococcus lactis combined with alcohol dehydrogenase gene (adh2, adha, an ... | 2016 | 26961908 |
| engineering of corynebacterium glutamicum for high-yield l-valine production under oxygen deprivation conditions. | we previously demonstrated efficient l-valine production by metabolically engineered corynebacterium glutamicum under oxygen deprivation. to achieve the high productivity, a nadh/nadph cofactor imbalance during the synthesis of l-valine was overcome by engineering nad-preferring mutant acetohydroxy acid isomeroreductase (ahair) and using nad-specific leucine dehydrogenase from lysinibacillus sphaericus. lactate as a by-product was largely eliminated by disrupting the lactate dehydrogenase gene l ... | 2013 | 23241971 |
| biotransformation of oleic acid into 10-ketostearic acid by recombinant corynebacterium glutamicum-based biocatalyst. | to produce 10-ketostearic acid from oleic acid. | 2015 | 25700814 |
| whole cell bioconversion of ricinoleic acid to 12-ketooleic acid by recombinant corynebacterium glutamicum-based biocatalyst. | the biocatalytic efficiency of recombinant corynebacterium glutamicum atcc 13032 expressing the secondary alcohol dehydrogenase of micrococcus luteus nctc2665 was studied. recombinant c. glutamicum converts ricinoleic acid to a product, identified by gas chromatography/mass spectrometry as 12-ketooleic acid (12-oxo-cis-9-octadecenoic acid). the effects of ph, reaction temperature, and non-ionic detergent on recombinant c. glutamiucm whole cell bioconversion were examined. the determined optimal ... | 2015 | 25639721 |
| gram-positive bacterial lipoglycans based on a glycosylated diacylglycerol lipid anchor are microbe-associated molecular patterns recognized by tlr2. | innate immune recognition is the first line of host defense against invading microorganisms. it is a based on the detection, by pattern recognition receptors (prrs), of invariant molecular signatures that are unique to microorganisms. tlr2 is a prr that plays a major role in the detection of gram-positive bacteria by recognizing cell envelope lipid-linked polymers, also called macroamphiphiles, such as lipoproteins, lipoteichoic acids and mycobacterial lipoglycans. these microbe-associated molec ... | 2013 | 24278450 |
| production and glucosylation of c50 and c 40 carotenoids by metabolically engineered corynebacterium glutamicum. | the yellow-pigmented soil bacterium corynebacterium glutamicum atcc13032 is accumulating the cyclic c50 carotenoid decaprenoxanthin and its glucosides. carotenoid pathway engineering was previously shown to allow for efficient lycopene production. here, engineering of c. glutamicum for production of endogenous decaprenoxanthin as well as of the heterologous c50 carotenoids c.p.450 and sarcinaxanthin is described. plasmid-borne overexpression of genes for lycopene cyclization and hydroxylation fr ... | 2014 | 24270893 |
| structural basis for cytokinin production by log from corynebacterium glutamicum. | "lonely guy" (log) has been identified as a cytokinin-producing enzyme in plants and plant-interacting fungi. the gene product of cg2612 from the soil-dwelling bacterium corynebacterium glutamicum was annotated as an ldc. however, the facts that c. glutamicum lacks an ldc and cg2612 has high amino acid similarity with log proteins suggest that cg2612 is possibly an log protein. to investigate the function of cg2612, we determined its crystal structure at a resolution of 2.3 å. cg2612 functions a ... | 2016 | 27507425 |
| the antituberculosis drug ethambutol selectively blocks apical growth in cmn group bacteria. | members of the genus mycobacterium are the most prevalent cause of infectious diseases. mycobacteria have a complex cell envelope containing a peptidoglycan layer and an additional arabinogalactan polymer to which a mycolic acid bilayer is linked; this complex, multilayered cell wall composition (magp) is conserved among all cmn group bacteria. the arabinogalactan and mycolic acid synthesis pathways constitute effective drug targets for tuberculosis treatment. ethambutol (emb), a classical antit ... | 2017 | 28174310 |
| identification of a membrane protein required for lipomannan maturation and lipoarabinomannan synthesis in corynebacterineae. | mycobacterium tuberculosis and related corynebacterineae synthesize a family of lipomannans (lm) and lipoarabinomannans (lam) that are abundant components of the multilaminate cell wall and essential virulence factors in pathogenic species. here we describe a new membrane protein, highly conserved in all corynebacterineae, that is required for synthesis of full-length lm and lam. deletion of the corynebacterium glutamicum ncgl2760 gene resulted in a complete loss of mature lm/lam and the appeara ... | 2017 | 28167532 |
| structure and function of amtr in mycobacterium smegmatis: implications for post-transcriptional regulation of urea metabolism through a small antisense rna. | soil-dwelling bacteria of the phylum actinomycetes generally harbor either glnr or amtr as a global regulator of nitrogen metabolism. mycobacterium smegmatis harbors both of these canonical regulators; glnr regulates the expression of key genes involved in nitrogen metabolism, while the function and signal transduction pathway of amtr in m. smegmatis remains largely unknown. here, we report the structure and function of the m. smegmatis amtr and describe the role of amtr in the regulation of nit ... | 2016 | 27640309 |
| synthesis, structural elucidation, and biochemical analysis of immunoactive glucuronosyl diacylglycerides of mycobacteria and corynebacteria. | glucuronosyl diacylglycerides (glcagroac2) are functionally important glycolipids and membrane anchors for cell wall lipoglycans in the corynebacteria. here we describe the complete synthesis of distinct acyl-isoforms of glcagroac2 bearing both acylation patterns of (r)-tuberculostearic acid (c19:0) and palmitic acid (c16:0) and their mass spectral characterization. collision-induced fragmentation mass spectrometry identified characteristic fragment ions that were used to develop "rules" allowin ... | 2013 | 23343519 |
| mmpl genes are associated with mycolic acid metabolism in mycobacteria and corynebacteria. | mycolic acids are vital components of the cell wall of the tubercle bacillus mycobacterium tuberculosis and are required for viability and virulence. while mycolic acid biosynthesis is studied extensively, components involved in mycolate transport remain unidentified. we investigated the role of large membrane proteins encoded by mmpl genes in mycolic acid transport in mycobacteria and the related corynebacteria. mmpl3 was found to be essential in mycobacteria and conditional depletion of mmpl3 ... | 2012 | 22520756 |
| corrigendum: cell division in corynebacterineae. | [this corrects the article on p. 132 in vol. 5, pmid: 24782835.]. | 2016 | 27990143 |
| structure-function profile of mmpl3, the essential mycolic acid transporter from mycobacterium tuberculosis. | the mmpl family of proteins translocates complex (glyco)lipids and siderophores across the cell envelope of mycobacteria and closely related corynebacteriaceae and plays important roles in the biogenesis of the outer membrane of these organisms. despite their significance in the physiology and virulence of mycobacterium tuberculosis, and from the perspective of developing novel antituberculosis agents, little is known about their structure and mechanism of translocation. in this study, the essen ... | 2016 | 27737557 |
| assembling of the mycobacterium tuberculosis cell wall core. | the unique cell wall of mycobacteria is essential to their viability and the target of many clinically used anti-tuberculosis drugs and inhibitors under development. despite intensive efforts to identify the ligase(s) responsible for the covalent attachment of the two major heteropolysaccharides of the mycobacterial cell wall, arabinogalactan (ag) and peptidoglycan (pg), the enzyme or enzymes responsible have remained elusive. we here report on the identification of the two enzymes of mycobacter ... | 2016 | 27417139 |
| crystal structure and biochemical characterization of tetrahydrodipicolinate n-succinyltransferase from corynebacterium glutamicum. | tetrahydrodipicolinate n-succinyltransferase (dapd) is an enzyme involved in the biosynthesis of l-lysine by converting tetrahydrodipicolinate into n-succinyl-l-2-amino-6-oxopimelate, using succinyl-coa as a cofactor. we determined the crystal structure of dapd from corynebacterium glutamicum (cgdapd). cgdapd functions as a trimer, and each monomer consists of three domains: an n-terminal helical domain (ntd), a left-handed β-helix (lβh) domain, and a β c-terminal domain (ctd). the mode of cofac ... | 2015 | 26602189 |
| structural insight into dihydrodipicolinate reductase from corybebacterium glutamicum for lysine biosynthesis. | dihydrodipicolinate reductase is an enzyme that converts dihydrodipicolinate to tetrahydrodipicolinate using an nad(p)h cofactor in l-lysine biosynthesis. to increase the understanding of the molecular mechanisms of lysine biosynthesis, we determined the crystal structure of dihydrodipicolinate reductase from corynebacterium glutamicum (cgdapb). cgdapb functions as a tetramer, and each protomer is composed of two domains, an nterminal domain and a c-terminal domain. the n-terminal domain mainly ... | 2016 | 26502738 |
| cmregnet-an interspecies reference database for corynebacterial and mycobacterial regulatory networks. | organisms utilize a multitude of mechanisms for responding to changing environmental conditions, maintaining their functional homeostasis and to overcome stress situations. one of the most important mechanisms is transcriptional gene regulation. in-depth study of the transcriptional gene regulatory network can lead to various practical applications, creating a greater understanding of how organisms control their cellular behavior. | 2015 | 26062809 |
| lead selection and characterization of antitubercular compounds using the nested chemical library. | discovering new drugs to treat tuberculosis more efficiently and to overcome multidrug resistance is a world health priority. to find novel antitubercular agents several approaches have been used in various institutions worldwide, including target-based approaches against several validated mycobacterial enzymes and phenotypic screens. we screened more than 17,000 compounds from vichem's nested chemical library™ using an integrated strategy involving whole cell-based assays with corynebacterium g ... | 2015 | 25801335 |
| the crystal structures of apo and camp-bound glxr from corynebacterium glutamicum reveal structural and dynamic changes upon camp binding in crp/fnr family transcription factors. | the cyclic amp-dependent transcriptional regulator glxr from corynebacterium glutamicum is a member of the super-family of crp/fnr (cyclic amp receptor protein/fumarate and nitrate reduction regulator) transcriptional regulators that play central roles in bacterial metabolic regulatory networks. in c. glutamicum, which is widely used for the industrial production of amino acids and serves as a non-pathogenic model organism for members of the corynebacteriales including mycobacterium tuberculosis ... | 2014 | 25469635 |
| acyl-coa sensing by fasr to adjust fatty acid synthesis in corynebacterium glutamicum. | corynebacterium glutamicum, like mycobacterium tuberculosis, is a member of the corynebacteriales, which have linear fatty acids and as branched fatty acids the mycolic acids. we identified accd1 and fasa as key genes of fatty acid synthesis, encoding the β-subunit of the acetyl-coa carboxylase and a type-i fatty acid synthase, respectively, and observed their repression during growth on minimal medium with acetate. we also identified the transcriptional regulator fasr and its binding sites in t ... | 2014 | 25449109 |
| interaction of 2-oxoglutarate dehydrogenase odha with its inhibitor odhi in corynebacterium glutamicum: mutants and a model. | pyruvate dehydrogenase and oxoglutarate dehydrogenase catalyze key reactions in central metabolism. in corynebacterium glutamicum and related bacteria like mycobacterium tuberculosis both activities reside in a novel protein supercomplex with the fusion protein odha catalyzing the conversion of oxoglutarate to succinyl-coenzyme a. this activity is inhibited by the forkhead-associated (fha) domain of the small autoinhibitory protein odhi. here we used a biological screen which enabled us to isola ... | 2014 | 24905147 |
| benzothiazinones mediate killing of corynebacterineae by blocking decaprenyl phosphate recycling involved in cell wall biosynthesis. | benzothiazinones (btzs) are a new class of sulfur containing heterocyclic compounds that target dpre1, an oxidoreductase involved in the epimerization of decaprenyl-phosphoribose (dpr) to decaprenyl-phosphoarabinose (dpa) in the corynebacterineae, such as corynebacterium glutamicum and mycobacterium tuberculosis. as a result, btz inhibition leads to inhibition of cell wall arabinan biosynthesis. previous studies have demonstrated the essentiality of dpre1. in contrast, cg-ubia a ribosyltransfera ... | 2014 | 24446451 |
| the concerted action of a positive charge and hydrogen bonds dynamically regulates the pka of the nucleophilic cysteine in the nrdh-redoxin family. | nrdh-redoxins shuffle electrons from the nadph pool in the cell to class ib ribonucleotide reductases, which in turn provide the precursors for dna replication and repair. nrdh-redoxins have a cvqc active site motif and belong to the thioredoxin-fold protein family. as for other thioredoxin-fold proteins, the pk(a) of the nucleophilic cysteine of nrdh-redoxins is of particular interest since it affects the catalytic reaction rate of the enzymes. recently, the pk(a) value of this cysteine in cory ... | 2014 | 24243781 |
| sample preparation, crystallization and structure solution of hisc from mycobacterium tuberculosis. | histidinolphosphate aminotransferase (hisc; rv1600) from mycobacterium tuberculosis was overexpressed in m. smegmatis and purified to homogeneity using nickel-nitrilotriacetic acid metal-affinity and gel-filtration chromatography. diffraction-quality crystals suitable for x-ray analysis were grown by the hanging-drop vapour-diffusion technique using 30% polyethylene glycol monomethyl ether 2000 as the precipitant. the crystals belonged to the hexagonal space group p3221, with an unusual high sol ... | 2013 | 23545656 |
| interaction between dahp synthase and chorismate mutase endows new regulation on dahp synthase activity in corynebacterium glutamicum. | previous research on corynebacterium glutamicum revealed that 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase (dscg, formerly ds2098) interacts with chorismate mutase (cmcg, formerly cm0819). in this study, we investigated the interaction by means of structure-guided mutation and enzymatic assays. our results show that the interaction imparted a new mechanism for regulation of dahp activity: in the absence of cmcg, dscg activity was not regulated by prephenate, whereas in the presence of cm ... | 2013 | 23467831 |
| nrdh-redoxin of mycobacterium tuberculosis and corynebacterium glutamicum dimerizes at high protein concentration and exclusively receives electrons from thioredoxin reductase. | nrdh-redoxins are small reductases with a high amino acid sequence similarity with glutaredoxins and mycoredoxins but with a thioredoxin-like activity. they function as the electron donor for class ib ribonucleotide reductases, which convert ribonucleotides into deoxyribonucleotides. we solved the x-ray structure of oxidized nrdh-redoxin from corynebacterium glutamicum (cg) at 1.5 å resolution. based on this monomeric structure, we built a homology model of nrdh-redoxin from mycobacterium tuberc ... | 2013 | 23362277 |
| differential arabinan capping of lipoarabinomannan modulates innate immune responses and impacts t helper cell differentiation. | toll-like receptors (tlrs) recognize pathogens by interacting with pathogen-associated molecular patterns, such as the phosphatidylinositol-based lipoglycans, lipomannan (lm) and lipoarabinomannan (lam). such structures are present in several pathogens, including mycobacterium tuberculosis, being important for the initiation of immune responses. it is well established that the interaction of lm and lam with tlr2 is a process dependent on the structure of the ligands. however, the implications of ... | 2012 | 23144457 |
| the lipoprotein lpqw is essential for the mannosylation of periplasmic glycolipids in corynebacteria. | phosphatidylinositol mannosides (pim), lipomannan (lm), and lipoarabinomannan (lam) are essential components of the cell wall and plasma membrane of mycobacteria, including the human pathogen mycobacterium tuberculosis, as well as the related corynebacterineae. we have previously shown that the lipoprotein, lpqw, regulates pim and lm/lam biosynthesis in mycobacteria. here, we provide direct evidence that lpqw regulates the activity of key mannosyltransferases in the periplasmic leaflet of the ce ... | 2012 | 23091062 |
| deletion of manc in corynebacterium glutamicum results in a phospho-myo-inositol mannoside- and lipoglycan-deficient mutant. | mannose is an important constituent of the immunomodulatory glycoconjugates of the mycobacterial cell wall: lipoarabinomannan (lam), lipomannan (lm) and the related phospho-myo-inositol mannosides (pims). in mycobacterium tuberculosis and the related bacillus corynebacterium glutamicum, mannose is either imported from the medium or derived from glycolysis, and is subsequently converted into the nucleotide-based sugar donor guanosine diphosphomannose (gdp-mannose). this can be utilized by the gly ... | 2012 | 22539165 |
| novel and tightly regulated resorcinol and cumate-inducible expression systems for streptomyces and other actinobacteria. | inducible expression is a versatile genetic tool for controlling gene transcription, determining gene functions and other uses. herein, we describe our attempts to create several inducible systems based on a cumate or a resorcinol switch, a hammerhead ribozyme, the laci repressor, and isopropyl β-d-thiogalactopyranoside (iptg). we successfully developed a new cumate (p-isopropylbenzoic acid)-inducible gene switch in actinobacteria that is based on the cymr regulator, the operator sequence (cmt) ... | 2014 | 25012786 |
| hyaluronic acid production with corynebacterium glutamicum: effect of media composition on yield and molecular weight. | corynebacterium glutamicum was tested as an alternative host for heterologous production of hyaluronic acid (ha). | 2014 | 24863652 |
| engineering of corynebacterium glutamicum to utilize methyl acetate, a potential feedstock derived by carbonylation of methanol with co. | the possibilities to utilize one-carbon substrates (c1) like co, methane and methanol have been explored as a cheap alternative feedstock in the biotechnology. for the first time, methyl acetate (meoac), which can be formed from carbonylation of methanol with co, was demonstrated to be an alternative carbon source for the cell growth of corynebacterium glutamicum as a model microbial cell factory. to do so, a carboxyl esterase activity was necessary to hydrolyze meoac to methanol and acetate. al ... | 2016 | 26970052 |
| high-level expression in corynebacterium glutamicum of nitrile hydratase from rhodococcus rhodochrous for acrylamide production. | the nhhbag gene of rhodococcus rhodochrous m33 that encodes nitrile hydratase (nhase), converting acrylonitrile into acrylamide, was cloned and expressed in corynebacterium glutamicum under the control of an ilvc promoter. the specific enzyme activity in recombinant c. glutamicum cells was about 13.6 μmol/min/mg dry cell weight (dcw). to overexpress the nhase, five types of plasmid variants were constructed by introducing mutations into 80 nucleotides near the translational initiation region (ti ... | 2014 | 24493572 |
| production of orotic acid by a klura3δ mutant of kluyveromyces lactis. | we demonstrated that a klura3δ, mutant of the yeast kluyveromyces lactis is able to produce and secrete into the growth medium considerable amounts of orotic acid. using yeast extract-peptone-glucose (ypd) based media we optimized production conditions in flask and bioreactor cultures. with cells grown in ypd 5% glucose medium, the best production in flask was obtained with a 1:12.5 ratio for flask: culture volume, 180 rpm, 28°c and 200 mm mops for ph stabilization at neutral values (initial cul ... | 2016 | 26707627 |
| molecular characterization of a eukaryotic-like phenol hydroxylase from corynebacterium glutamicum. | this study focuses on the genetic and biochemical characterization of phenol hydroxylase (phe, ncgl2588) from corynebacterium glutamicum that shares 31% identity in amino acids with phenol hydroxylase from yeast trichosporon cutaneum but less similarity with that from bacteria. the phe deletion mutant significantly reduced its ability to grow with phenol as the sole carbon and energy source. expression of the phe gene was strongly induced with phenol and also subject to the control of carbon cat ... | 2015 | 26377129 |
| comparative proteome analysis of global effect of pos5 and zwf-ppnk overexpression in l-isoleucine producing corynebacterium glutamicum ssp. lactofermentum. | corynebacterium glutamicum ssp. lactofermentum strain jhi3-156 produces l-isoleucine (ile). overexpression of the saccharomyces cerevisiae-derived nadh kinase gene (pos5) and the endogenous glucose-6-phosphate dehydrogenase and nad kinase genes (zwf-ppnk) in jhi3-156 increased ile production by 26 and 31 %, respectively. to decipher the global effect of pos5 and zwf-ppnk overexpression on ile biosynthesis, proteomic analysis was conducted. twenty-four differentially expressed proteins were ident ... | 2015 | 25650341 |
| absolute quantification of corynebacterium glutamicum glycolytic and anaplerotic enzymes by qconcat. | the soil bacterium corynebacterium glutamicum is one of the best-studied production hosts for industrial biotechnology, and it is primarily used for the large-scale production of essential amino acids, such as l-lysine. for rational strain development, detailed knowledge of intracellular protein concentration is crucial to determine metabolic capacities and limitations. we developed a qconcat approach for the accurate absolute quantification of key enzymes of c. glutamicum glycolysis and anapler ... | 2015 | 25451015 |
| expression of nad(h) kinase and glucose-6-phosphate dehydrogenase improve nadph supply and l-isoleucine biosynthesis in corynebacterium glutamicum ssp. lactofermentum. | corynebacterium glutamicum is the workhorse for the production of amino acids, including l-isoleucine (ile). during ile biosynthesis, nadph is required as a crucial cofactor. in this study, four nadph-supplying strategies based on nad kinase, nadh kinase, glucose-6-phosphate dehydrogenase, and nad kinase coupling with glucose-6-phosphate dehydrogenase were compared, and their influences on ile biosynthesis were examined. ppnk is a nad kinase of c. glutamicum ssp. lactofermentum jhi3-156 that pre ... | 2013 | 23868449 |
| recent advancements in various steps of ethanol, butanol, and isobutanol productions from woody materials. | in this review, the recent advancements and technical challenges associated with the production of ethanol, butanol, and isobutanol via bioconversion routes from celluloses of woody materials are reviewed. physicochemical processes, e.g. steam explosion, seem to be the most viable process for pretreating woody materials. although enzymatic hydrolysis is selective, it is rather a slow process. acid hydrolysis is a relatively fast process with a high yield, but it produces inhibitory compounds of ... | 2013 | 23297056 |
| growth response of avena sativa in amino-acids-rich soils converted from phenol-contaminated soils by corynebacterium glutamicum. | the biodegradation of phenol in laboratory-contaminated soil was investigated using the gram-positive soil bacterium corynebacterium glutamicum. this study showed that the phenol degradation caused by c. glutamicum was greatly enhanced by the addition of 1% yeast extract. from the toxicity test using daphnia magna, the soil did not exhibit any hazardous effects after the phenol was removed using c. glutamicum. additionally, the treatment of the phenolcontaminated soils with c. glutamicum increas ... | 2012 | 22534303 |
| corynebacterium glutamicum possesses β-n-acetylglucosaminidase. | in gram-positive corynebacterium glutamicum and other members of the suborder corynebacterianeae, which includes mycobacteria, cell elongation and peptidoglycan biosynthesis is mainly due to polar growth. c. glutamicum lacks an uptake system for the peptidoglycan constituent n-acetylglucosamine (glcnac), but is able to catabolize glcnac-6-phosphate. due to its importance in white biotechnology and in order to ensure more sustainable processes based on non-food renewables and to reduce feedstock ... | 2016 | 27492186 |
| l-lysine production independent of the oxidative pentose phosphate pathway by corynebacterium glutamicum with the streptococcus mutans gapn gene. | we have recently developed a corynebacterium glutamicum strain that generates nadph via the glycolytic pathway by replacing endogenous nad-dependent glyceraldehyde 3-phosphate dehydrogenase (gapa) with a nonphosphorylating nadp-dependent glyceraldehyde 3-phosphate dehydrogenase (gapn) from streptococcus mutans. strain re2, a suppressor mutant spontaneously isolated for its improved growth on glucose from the engineered strain, was proven to be a high-potential host for l-lysine production (taken ... | 2016 | 27044449 |
| 3-amino-4-hydroxybenzoic acid production from sweet sorghum juice by recombinant corynebacterium glutamicum. | the production of the bioplastic precursor 3-amino-4-hydroxybenzoic acid (3,4-ahba) from sweet sorghum juice, which contains amino acids and the fermentable sugars sucrose, glucose and fructose, was assessed to address the limitations of producing bio-based chemicals from renewable feedstocks. recombinant corynebacterium glutamicum strain kt01 expressing grih and grii derived from streptomyces griseus produced 3,4-ahba from the sweet sorghum juice of cultivar sil-05 at a final concentration (1.0 ... | 2015 | 26409852 |
| co-expression of endoglucanase and β-glucosidase in corynebacterium glutamicum dm1729 towards direct lysine fermentation from cellulose. | the aim of the present study is the development of a consolidated bioprocess for the production of lysine with recombinant corynebacterium glutamicum dm1729 strains expressing endoglucanase and β-glucosidase genes. here, the endoglucanase genes from xanthomonas campestris xcc3521 and xcc2387 and betaglucosidase gene from saccharophagus degradans sde1394 were cloned in c. glutamicum dm1729 and expressed either extracellularly or on cell surface. the highest β-glucosidase activity of 9±0.5u/od600 ... | 2016 | 27020126 |
| production of para-aminobenzoate by genetically engineered corynebacterium glutamicum and non-biological formation of an n-glucosyl byproduct. | para-aminobenzoate (paba), a valuable chemical raw material, can be synthesized by most microorganisms. this aromatic compound is currently manufactured from petroleum-derived materials by chemical synthesis. to produce paba from renewable resources, its production by fermentation was investigated. the evaluation of the sensitivity to paba toxicity revealed that corynebacterium glutamicum had better tolerance to paba than several other microorganisms. to produce paba from glucose, genetically en ... | 2016 | 27471069 |
| biosynthesis of pinene from glucose using metabolically-engineered corynebacterium glutamicum. | pinene is a monoterpenes (c10) that is produced in a genetically-engineered microbial host for its industrial applications in fragrances, flavoring agents, pharmaceuticals, and biofuels. herein, we have metabolically-engineered corynebacterium glutamicum, to produce pinene and studied its toxicity in c. glutamicum. geranyl diphosphate synthases (gpps) and pinene synthases (ps), obtained from pinus taeda and abies grandis, were co-expressed with over-expressed native 1-deoxy-d-xylulose-5-phosphat ... | 2014 | 24930112 |
| breeding l-arginine-producing strains by a novel mutagenesis method: atmospheric and room temperature plasma (artp). | a plasma jet, driven by an active helium atom supplied with an atmospheric and room temperature plasma (artp) biological breeding system, was used as a novel method to breed l-arginine high-yielding strains. a mutant with resistance to l-homoarginine and 8-azaguaine, arg 3-15 (l-ha(r), 8-ag(r), l-his(-)), was screened after several rounds of screening. the l-arginine production of these mutants was more than that of the original strain, increased by 43.79% for arg 3-15. moreover, n-acetyl-l-glut ... | 2016 | 26460578 |
| development of a new platform for secretory production of recombinant proteins in corynebacterium glutamicum. | corynebacterium glutamicum, which has been for long an industrial producer of various l-amino acids, nucleic acids, and vitamins, is now also regarded as a potential host for the secretory production of recombinant proteins. to harness its potential as an industrial platform for recombinant protein production, the development of an efficient secretion system is necessary. particularly, regarding protein production in large-scale bioreactors, it would be appropriate to develop a secretory express ... | 2016 | 26134574 |
| mutagenesis and redox partners analysis of the p450 fatty acid decarboxylase oletje. | the cytochrome p450 enzyme oletje from jeotgalicoccus sp. atcc 8456 is capable of converting free long-chain fatty acids into α-alkenes via one-step oxidative decarboxylation in presence of h2o2 as cofactor or using redox partner systems. this enzyme has attracted much attention due to its intriguing but unclear catalytic mechanism and potential application in biofuel production. here, we investigated the functionality of a select group of residues (arg245, cys365, his85, and ile170) in the acti ... | 2017 | 28276499 |
| corynebacterium crudilactis sp. nov., isolated from raw cow's milk. | a gram-stain-positive, rod-shaped bacterium (strain jz16t) was isolated from raw cow's milk from the bulk tank of a dairy farm in germany. the 16s rrna gene sequence of the isolate showed a similarity of 98.3 % to the nearest related type strain corynebacterium glutamicum atcc 13032t, a similarity of 97.6 % to corynebacterium deserti gimn1.010t and a similarity of 97.4 % to corynebacterium callunae dsm 20147t. determination of chemotaxonomic characteristics revealed oleic acid (18 : 1 cis 9) as ... | 2016 | 27666312 |
| efficient hydroxyproline production from glucose in minimal media by corynebacterium glutamicum. | the efficient coupling of biotransformation steps to an existing fermentation pathway is an interesting strategy to expand the product portfolio of corynebacterium glutamicum as whole-cell biocatalyst. this is especially challenging if the biotransformation step comprises a direct link to central metabolism, as in the case of α-ketoglutarate-dependent oxygenase catalysis. aiming at trans-4-hydroxy-l-proline (hyp) production from glucose in a minimal medium, the proline-4-hydroxylase gene from da ... | 2015 | 25163732 |
| [cost-effective production of protein by using cellulose-binding domain fusion tag in corynebacterium glutamicum]. | the cbd gene from trichoderma reesei was cloned into the corynebacterium glutamicum secretion expression vector pxmj19-sp, in which green fluorescent protein was inserted to obtain pxmj19-sp-gfp-cbd. after induced by 0.5 mmol/l iptg, gfp-cbd was expressed in corynebacterium glutamicum at high level of 200 mg/l. the gfp-cbd could be purified to high purity with cellulose column. the results indicated cbd can be successfully used in corynebacterium glutamicum expression system and thus offer an ex ... | 2013 | 24010367 |
| sequence-based identification of inositol monophosphatase-like histidinol-phosphate phosphatases (hisn) in corynebacterium glutamicum, actinobacteria, and beyond. | the eighth step of l-histidine biosynthesis is carried out by an enzyme called histidinol-phosphate phosphatase (holpase). three unrelated holpase families are known so far. two of them are well studied: had-type holpases known from gammaproteobacteria like escherichia coli or salmonella enterica and php-type holpases known from yeast and firmicutes like bacillus subtilis. however, the third family of holpases, the inositol monophosphatase (impase)-like holpases, present in actinobacteria like c ... | 2017 | 28720084 |
| a truncated sph12-38 with potent antimicrobial activity showing resistance against bacterial challenge in oryzias melastigma. | antimicrobial peptides (amps) represent an efficient part of innate immunity and are found in a variety of life. among them histone 2a (h2a), as a promising class of amps, attracts great attention, but the in vivo mechanism of h2a derived amp is still less known. based on the acquisition of sphistin, a synthetic 38-amino acid h2a derived peptide from scylla paramamosain, as reported in our previous study, was truncated into three short fragments (sph12-38, sph20-38 and sph30-38) and further inve ... | 2017 | 28600196 |
| 1,5-diaminopentane production from xylooligosaccharides using metabolically engineered corynebacterium glutamicum displaying beta-xylosidase on the cell surface. | xylooligosaccharide-assimilating corynebacterium glutamicum strains were constructed using metabolic engineering and cell surface display techniques. first, c. glutamicum was metabolically engineered to create lysine-producing strains. beta-xylosidase bsu17580 derived from bacillus subtilis was then expressed on the c. glutamicum cell surface using porh anchor protein, and enzymes involved in the xylose assimilation pathway were also expressed. metabolic engineering had no effect on the activity ... | 2017 | 28599919 |
| molecular engineering of l-aspartate-α-decarboxylase for improved activity and catalytic stability. | β-alanine is an important precursor for the production of food additives, pharmaceuticals, and nitrogen-containing chemicals. compared with the conventional chemical routes for β-alanine production, the biocatalytic routes using l-aspartate-α-decarboxylase (adc) are more attractive when energy and environment are concerned. however, adc's poorly understood properties and its inherent mechanism-based inactivation significantly limited the application of this enzyme. in this study, three genes enc ... | 2017 | 28589224 |
| development of a bacterial biosensor for rapid screening of yeast p-coumaric acid production. | transcription factor-based biosensors are used to identify producer strains, a critical bottleneck in cell factory engineering. here, we address two challenges with this methodology: transplantation of heterologous transcriptional regulators into new hosts to generate functional biosensors and biosensing of the extracellular product concentration that accurately reflects the effective cell factory production capacity. we describe the effects of different translation initiation rates on the dynam ... | 2017 | 28532147 |
| comparative analysis of polyspecificity of the endogenous trna synthetase of different expression host towards photocrosslinking amino acids using an in silico approach. | photo-induced covalent crosslinking has emerged as the powerful strategy for analyzing and characterizing the protein-protein interaction and mapping protein 3d conformations. in the last decades, a number of photocrosslinking amino acids have been reported but only a few have been efficiently utilized for photocrosslinking purposes. recently, incorporation of diazirine containing photoactivatable analogs such as photo-methionine, photo-leucine, photo-isoleucine and photo-lysine into target prot ... | 2017 | 28641210 |
| enhancing poly-γ-glutamic acid production in bacillus amyloliquefaciens by introducing the glutamate synthesis features from corynebacterium glutamicum. | poly-γ-glutamic acid (γ-pga) is a valuable polymer with glutamate as its sole precursor. enhancement of the intracellular glutamate synthesis is a very important strategy for the improvement of γ-pga production, especially for those glutamate-independent γ-pga producing strains. corynebacterium glutamicum has long been used for industrial glutamate production and it exhibits some unique features for glutamate synthesis; therefore introduction of these metabolic characters into the γ-pga producin ... | 2017 | 28532451 |
| in vivo roles of fatty acid-biosynthetic enzymes in biosynthesis of biotin and α-lipoic acid in corynebacterium glutamicum. | for fatty acid biosynthesis, corynebacterium glutamicum uses two type i fatty acid synthases (fas-i), fasa and fasb, in addition to acetyl-coa carboxylase (acc) consisting of accbc, accd1, and acce. the in vivo roles of the enzymes in supplying precursors for biotin and α-lipoic acid remain unclear. here, we report genetic evidence demonstrating that the biosynthesis of these cofactors is linked to fatty acid biosynthesis through the fas-i pathway. for this study, we used wild-type c. glutamicum ... | 2017 | 28754705 |
| engineering of corynebacterium glutamicum for consolidated conversion of hemicellulosic biomass into xylonic acid. | xylonic acid is a promising platform chemical with various applications in the fields of food, pharmaceuticals, and agriculture. however, in the current process, xylonic acid is mainly produced by the conversion of xylose, whose preparation requires substantial cost and time. here, we engineered corynebacterium glutamicum for the consolidated bioconversion of hemicellulosic biomass (xylan) into xylonic acid in a single cultivation. first, for the efficient conversion of xylose to xylonic acid, x ... | 2017 | 28799725 |
| engineering of corynebacterium glutamicum for minimized carbon loss during utilization of d-xylose containing substrates. | biomass-derived d-xylose represents an economically interesting substrate for the sustainable microbial production of value-added compounds. the industrially important platform organism corynebacterium glutamicum has already been engineered to grow on this pentose as sole carbon and energy source. however, all currently described c. glutamicum strains utilize d-xylose via the commonly known isomerase pathway that leads to a significant carbon loss in the form of co2, in particular, when aiming f ... | 2014 | 25304460 |
| construction of recombinant pdu metabolosome shells for small molecule production in corynebacterium glutamicum. | bacterial microcompartments have significant potential in the area of industrial biotechnology for the production of small molecules, especially involving metabolic pathways with toxic or volatile intermediates. corynebacterium glutamicum is an established industrial workhorse for the production of amino acids and has been investigated for the production of a variety of further value-added products. herein, we describe components for the establishment of bacterial microcompartments as production ... | 2017 | 28826205 |
| escherichia coli yjjpb genes encode a succinate transporter important for succinate production. | under anaerobic conditions, escherichia coli produces succinate from glucose via the reductive tricarboxylic acid cycle. to date, however, no genes encoding succinate exporters have been established in e. coli. therefore, we attempted to identify genes encoding succinate exporters by screening an e. coli mg1655 genome library. we identified the yjjpb genes as candidates encoding a succinate transporter, which enhanced succinate production in pantoea ananatis under aerobic conditions. a complemen ... | 2017 | 28673128 |
| current status on metabolic engineering for the production of l-aspartate family amino acids and derivatives. | the l-aspartate amino acids (afaas) are constituted of l-aspartate, l-lysine, l-methionine, l-threonine and l-isoleucine. except for l-aspartate, afaas are essential amino acids that cannot be synthesized by humans and most farm animals, and thus possess wide applications in food, animal feed, pharmaceutical and cosmetics industries. to date, a number of amino acids, including afaas have been industrially produced by microbial fermentation. however, the overall metabolic and regulatory mechanism ... | 2017 | 28579173 |
| production of amino acids - genetic and metabolic engineering approaches. | the biotechnological production of amino acids occurs at the million-ton scale and annually about 6milliontons of l-glutamate and l-lysine are produced by escherichia coli and corynebacterium glutamicum strains. l-glutamate and l-lysine production from starch hydrolysates and molasses is very efficient and access to alternative carbon sources and new products has been enabled by metabolic engineering. this review focusses on genetic and metabolic engineering of amino acid producing strains. in p ... | 2017 | 28552565 |
| a new metabolic route for the fermentative production of 5-aminovalerate from glucose and alternative carbon sources. | here, a new metabolic pathway for the production of 5-aminovalerate (5ava) from l-lysine via cadaverine as intermediate was established and this three-step-pathway comprises l-lysine decarboxylase (ldcc), putrescine transaminase (pata) and γ-aminobutyraldehyde dehydrogenase (patd). since corynebacterium glutamicum is used for industrial l-lysine production, the pathway was established in this bacterium. upon expression of ldcc, pata and patd from escherichia coli in c. glutamicum wild type, prod ... | 2017 | 28522202 |
| comparative analysis of the expression level of recombinant ginsenoside-transforming β-glucosidase in gras hosts and mass production of the ginsenoside rh2-mix. | the ginsenoside rh2, a pharmaceutically active component of ginseng, is known to have anticancer and antitumor effects. however, white ginseng and red ginseng have extremely low concentrations of rh2 or rh2-mix [20(s)-rh2, 20(r)-rh2, rk2, and rh3]. to enhance the production of food-grade ginsenoside rh2, an edible enzymatic bioconversion technique was developed adopting gras host strains. a β-glucosidase (bglpm), which has ginsenoside conversion ability, was expressed in three gras host strains ... | 2017 | 28423055 |
| functional analysis of arabinofuranosidases and a xylanase of corynebacterium alkanolyticum for arabinoxylan utilization in corynebacterium glutamicum. | xylooligosaccharides (xoss) and arabinoxylooligosaccharides (axoss) are major oligosaccharides derived from arabinoxylan. in our previous report, corynebacterium glutamicum was engineered to utilize xoss by introducing corynebacterium alkanolyticum xyloside transporter and β-xylosidase. however, this strain was unable to consume axoss due to the absence of α-l-arabinofuranosidase activity. in this study, to confer axos utilization ability on c. glutamicum, two putative arabinofuranosidase genes ... | 2017 | 28409383 |
| engineering a lysine-on riboswitch for metabolic control of lysine production in corynebacterium glutamicum. | riboswitches are natural rna elements that regulate gene expression by binding a ligand. here, we demonstrate the possibility of altering a natural lysine-off riboswitch from eschericia coli (ecrs) to a synthetic lysine-on riboswitch and using it for metabolic control. to this end, a lysine-on riboswitch library was constructed using teta-based dual genetic selection. after screening the library, the functionality of the selected lysine-on riboswitches was examined using a report gene, lacz. sel ... | 2015 | 26300047 |
| metabolic engineering of corynebacterium glutamicum for glycolate production. | corynebacterium glutamicum - a well-known industrial amino acid producer - has recently been engineered for the production of a variety of new products including diamines, alcohols, carotenoids and organic acids. glycolic acid was shown here not to serve as sole or combined carbon source for c. glutamicum. glycolate affected growth of c. glutamicum only at high concentrations (460mm) and in a comparable manner as other salts (480mm potassium chloride and 490mm sodium chloride). a transcriptome a ... | 2014 | 24486442 |
| modification of chimeric (2s, 3s)-butanediol dehydrogenase based on structural information. | a chimeric (2s, 3s)-butanediol dehydrogenase (clbdh) was engineered to have the strict (s)-configuration specificity of the (2s, 3s)-bdh (bslbdh) derived from brevibacterium saccharolyticum as well as the enzymatic stability of the (2r, 3s)-bdh (kpmbdh) from klebsiella pneumonia by swapping the domains of two native bdhs. however, while clbdh possesses the stability, it lacks the specificity. in order to assist in the design a bdh having strict substrate specificity, an x-ray structural analysis ... | 2014 | 25612804 |
| the antibacterial prodrug activator rv2466c is a mycothiol-dependent reductase in the oxidative stress response of mycobacterium tuberculosis. | the mycobacterium tuberculosis rv2466c gene encodes an oxidoreductase enzyme annotated as dsba. it has a cpwc active-site motif embedded within its thioredoxin fold domain and mediates the activation of the prodrug tp053, a thienopyrimidine derivative that kills both replicating and nonreplicating bacilli. however, its mode of action and actual enzymatic function in m. tuberculosis have remained enigmatic. in this study, we report that rv2466c is essential for bacterial survival under h2o2 stres ... | 2017 | 28620052 |
| production of 5-aminovaleric acid in recombinant corynebacterium glutamicum strains from a miscanthus hydrolysate solution prepared by a newly developed miscanthus hydrolysis process. | this study examined nine expired industrial corynebacterium glutamicum strains with high lysine producing capability for enhanced production of 5-ava. c. glutamicum kctc 1857 exhibiting the highest lysine production was transformed with either original pseudomonas putida davba genes, encoding the 5-ava biosynthesis pathway, or c. glutamicum codon-optimized davba genes. c. glutamicum kctc 1857 expressing the original genes had superior cell viability and 5-ava production capability compared to th ... | 2017 | 28579174 |
| bioprocess engineering to produce 9-(nonanoyloxy) nonanoic acid by a recombinant corynebacterium glutamicum-based biocatalyst. | here, corynebacterium glutamicum atcc13032 expressing baeyer-villiger monooxygenase from pseudomonas putida kt2440 was designed to produce 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid. diverse parameters including cultivation and reaction temperatures, type of detergent, and ph were found to improve biotransformation efficiency. the optimal temperature of cultivation for the production of 9-(nonanoyloxy) nonanoic acid from 10-ketostearic acid using whole cells of recombinant c. glutami ... | 2017 | 28567672 |
| bio-based production of dimethyl itaconate from rice wine waste-derived itaconic acid. | dimethyl itaconate is an important raw material for copolymerization, but it is not synthesized from itaconic acid by organisms. moreover, corynebacterium glutamicum is used as an important industrial host for the production of organic acids, but it does not metabolize itaconic acid. therefore, the biosynthetic route towards dimethyl itaconate from itaconic acid is highly needed. in this study, we developed a biological procedure for dimethyl itaconate production from rice wine waste-derived ita ... | 2017 | 28846199 |
| 5-aminolevulinic acid production in engineered corynebacterium glutamicum via c5 biosynthesis pathway. | ala (5-aminolevulinic acid) is an important intermediate in the synthesis of tetrapyrroles and the use of ala has been gradually increasing in many fields, including medicine and agriculture. in this study, improved biological production of ala in corynebacterium glutamicum was achieved by overexpressing glutamate-initiated c5 pathway. for this purpose, copies of the glutamyl t-rna reductase hema from several bacteria were mutated by site-directed mutagenesis of which a hema version from salmone ... | 2015 | 26453466 |
| rational design of substrate binding pockets in polyphosphate kinase for use in cost-effective atp-dependent cascade reactions. | adenosine-5'-triphosphate (atp) is the energy equivalent of the living system. polyphosphate (polyp) is the ancient energy storage equivalent of organisms. polyphosphate kinases (ppks) catalyze the polyp formation or atp formation, to store energy or to regenerate atp, respectively. however, most ppks are active only in the presence of long polyps, which are more difficult and more expensive to generate than the short polyps. we investigated the ppk preference towards polyps by site-directed mut ... | 2017 | 28417169 |
| crispr-cpf1 assisted genome editing of corynebacterium glutamicum. | corynebacterium glutamicum is an important industrial metabolite producer that is difficult to genetically engineer. although the streptococcus pyogenes (sp) crispr-cas9 system has been adapted for genome editing of multiple bacteria, it cannot be introduced into c. glutamicum. here we report a francisella novicida (fn) crispr-cpf1-based genome-editing method for c. glutamicum. crispr-cpf1, combined with single-stranded dna (ssdna) recombineering, precisely introduces small changes into the bact ... | 2017 | 28469274 |
| corynebacterium defluvii sp. nov., isolated from sewage. | a gram-positive, aerobic, non-motile, rod-shapeds, catalase-positive, and oxidase-negative strain, designated y49(t), was isolated from sewage collected from jilin agricultural university, china. it grew at 20-40°c (optimum at 30°c), at ph 6.0-8.0 (optimum at 7.0) and at 0-1.0% sodium chloride (optimum at 0%). the major isoprenoid quinone was menaquinone-8 (mk-8) and the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylmethylethanolamine, four unidentified lipids, and ... | 0 | 28429167 |
| comprehensive assessment of the l-lysine production process from fermentation of sugarcane molasses. | l-lysine is an essential amino acid that can be produced by chemical processes from fossil raw materials, as well as by microbial fermentation, the latter being a more efficient and environmentally friendly procedure. in this work, the production process of l-lysine-hcl is studied using a systematic approach based on modeling and simulation, which supports decision making in the early stage of process design. the study considers two analysis stages: first, the dynamic analysis of the fermentatio ... | 0 | 28409400 |
| quantitation of intracellular purine intermediates in different corynebacteria using electrospray lc-ms/ms. | intermediates of the purine biosynthesis pathway play key roles in cellular metabolism including nucleic acid synthesis and signal mediation. in addition, they are also of major interest to the biotechnological industry as several intermediates either possess flavor-enhancing characteristics or are applied in medical therapy. in this study, we have developed an analytical method for quantitation of 12 intermediates from the purine biosynthesis pathway including important nucleotides and their co ... | 2012 | 22960872 |
| the three-component system esrisr regulates a cell envelope stress response in corynebacterium glutamicum. | when the cell envelope integrity is compromised, bacteria trigger signaling cascades resulting in the production of proteins that counteract these extracytoplasmic stresses. here, we show that the two-component system esrsr regulates a cell envelope stress response in the actinobacterium corynebacterium glutamicum. the sensor kinase esrs possesses an amino-terminal phage shock protein c (pspc) domain, a property that sets esrsr apart from all other two-component systems characterized so far. an ... | 2017 | 28922502 |
| functional comparison of methionine sulphoxide reductase a and b in corynebacterium glutamicum. | methionine sulphoxide reductases (msr) are able to reduce methionine sulfoxide to methionine and protect bacteria against reactive oxygen species (ros). many organisms express both methionine sulphoxide reductase a (msra), specific for methionine-s-sulfoxide and methionine sulphoxide reductase b (msrb), active against methionine-r-sulfoxide. corynebacterium glutamicum expresses msra, the function of which has been well defined; however, the function of msrb has not been studied. whether msrb and ... | 2017 | 28904252 |
| effect of lysine succinylation on the regulation of 2-oxoglutarate dehydrogenase inhibitor, odhi, involved in glutamate production in corynebacterium glutamicum. | in corynebacterium glutamicum, the activity of the 2-oxoglutarate dehydrogenase (odh) complex is negatively regulated by the unphosphorylated form of odhi protein, which is critical for l-glutamate overproduction. we examined the potential impact of protein acylation at lysine (k)-132 of odhi in c. glutamicum atcc13032. the k132e succinylation-mimic mutation reduced the ability of odhi to bind odha, the catalytic subunit of the odh complex, which reduced the inhibition of odh activity. in vitro ... | 2017 | 28899215 |
| developing genome-reduced pseudomonas chlororaphis strains for the production of secondary metabolites. | the current chassis organisms or various types of cell factories have considerable advantages and disadvantages. therefore, it is necessary to develop various chassis for an efficient production of different bioproducts from renewable resources. in this context, synthetic biology offers unique potentialities to produce value-added products of interests. microbial genome reduction and modification are important strategies for constructing cellular chassis and cell factories. many genome-reduced s ... | 2017 | 28893188 |
| amino acids production focusing on fermentation technologies - a review. | amino acids are attractive and promising biochemicals with market capacity requirements constantly increasing. their applicability ranges from animal feed additives, flavour enhancers and ingredients in cosmetic to specialty nutrients in pharmaceutical and medical fields. this review gives an overview of the processes applied for amino acids production and points out the main advantages and disadvantages of each. due to the advances made in the genetic engineering techniques, the biotechnologica ... | 2017 | 28888551 |
| synthetic biology for manufacturing chemicals: constraints drive the use of non-conventional microbial platforms. | genetically modified microbes have had much industrial success producing protein-based products (such as antibodies and enzymes). however, engineering microbial workhorses for biomanufacturing of commodity compounds remains challenging. first, microbes cannot afford burdens with both overexpression of multiple enzymes and metabolite drainage for product synthesis. second, synthetic circuits and introduced heterologous pathways are not yet as "robust and reliable" as native pathways due to hosts' ... | 2017 | 28884354 |
| [biocatalytic access to β-alanine by a two-enzyme cascade synthesis]. | enzymatic synthesis is an important way to produce β-alanine, but the biological method is expensive generally because of the high price of substrate. in this paper, a two-step enzymatic cascade system was developed, combining l-aspartase from escherichia coli dh5α and l-aspartate α-decarboxylase from corynebacterium glutamicum. this system catalyzes fumarate and ammonia to β-alanine. the optimal ratio of aspa and pand was 1:80 (w/w), and the concentration of aspa was 10 μg/ml, at 37 ℃ and ph 7. ... | 2017 | 28876041 |
| identification and functional characterization of small alarmone synthetases in corynebacterium glutamicum. | the hyperphosphorylated guanosine derivatives ppgpp and pppgpp represent global regulators of the bacterial stress response, as they act as central elements of the stringent response system. although it was assumed that both, (p)ppgpp synthesis and hydrolysis, are catalyzed by one bifunctional rsh-protein in the actinobacterial model organism corynebacterium glutamicum atcc 13032, two putative short alarmone synthetases (sass) were identified by bioinformatic analyses. the predicted sequences of ... | 2017 | 28871248 |
| enhanced biosynthesis of hyaluronic acid using engineered corynebacterium glutamicum via metabolic pathway regulation. | hyaluronic acid (ha) is a polysaccharide used in many industries such as medicine, surgery, cosmetics and food. to avoid potential pathogenicity caused by its native producer, streptococcus, efforts have been made to create a recombinant host for ha production. in this work, a gras (generally recognized as safe) strain, corynebacterium glutamicum, was engineered for enhanced biosynthesis of ha via metabolic pathway regulation. five enzymes (hasa-hase) involved in the ha biosynthetic pathway were ... | 2017 | 28869338 |
| cgii cleaves dna using a mechanism distinct from other atp-dependent restriction endonucleases. | the restriction endonuclease cgli from corynebacterium glutamicum recognizes an asymmetric 5'-gccgc-3' site and cleaves the dna 7 and 6/7 nucleotides downstream on the top and bottom dna strands, respectively, in an ntp-hydrolysis dependent reaction. cgli is composed of two different proteins: an endonuclease (r.cgli) and a dead-family helicase-like atpase (h.cgli). these subunits form a heterotetrameric complex with r2h2 stoichiometry. however, the r2h2·cgli complex has only one nuclease active ... | 2017 | 28854738 |
| structure, function, and regulation of enzymes involved in amino acid metabolism of bacteria and archaea. | amino acids are essential components in all organisms because they are building blocks of proteins. they are also produced industrially and used for various purposes. for example, l-glutamate is used as the component of "umami" taste and lysine has been used as livestock feed. recently, many kinds of amino acids have attracted attention as biological regulators and are used for a healthy life. thus, to clarify the mechanism of how amino acids are biosynthesized and how they work as biological re ... | 2017 | 28840778 |
| ph fluctuations imperil the robustness of c. glutamicum to short term oxygen limitation. | the presence of complex gradients for, e.g., nutrients, oxygen or ph in industrial scale fed batch processes are a major challenge for process performance. to consider such impact of scale-up during laboratory scale process development, scale-down bioreactor simulation, i.e. mimicking inhomogeneous conditions, became the method of choice. however, most scale-down studies simulate combined inhomogeneities of more than one parameter, so that the impact of the individual parameters remains unclear. ... | 2017 | 28837821 |
| chemical shift assignments of polyketide cyclase_like protein cgl2373 from corynebacterium glutamicum. | protein cgl2373 from corynebacterium glutamicum, which is 155 amino acids long and 17.7 kda, is a member of the polyketide_cyc2 family. as a potential polyketide cyclase, it may play an important role in the biosynthesis of aromatic polyketides that are the source of many bioactive molecules. here we report the complete (1)h, (13)c and (15)n chemical shift assignments of cgl2373, which lays a foundation for further structural and functional research. | 2017 | 28825188 |
| polymerization dynamics of the prophage-encoded actin-like protein alpc is influenced by the dna-binding adapter alpa. | the corynebacterium glutamicum atcc 13032 prophage cgp3 encodes an actin-like protein, alpc that was shown to be involved in viral dna transport and efficient viral dna replication. alpc binds to an adapter, alpa that in turn binds to specific dna sequences, termed alps sites. thus, the alpac system is similar to the known plasmid segregation system parmrs. so far it is unclear how the alpacs system mediates dna transport and, whether alpa and alpc functionally interact. we show here that alpa m ... | 2017 | 28824563 |
| corynebacterium glutamicum chassis c1*: building and testing a novel platform host for synthetic biology and industrial biotechnology. | targeted top-down strategies for genome reduction are considered to have a high potential for providing robust basic strains for synthetic biology and industrial biotechnology. recently, we created a library of 26 genome-reduced strains of corynebacterium glutamicum carrying broad deletions in single gene clusters and showing wild-type-like biological fitness. here, we proceeded with combinatorial deletions of these irrelevant gene clusters in two parallel orders, and the resulting library of 28 ... | 2017 | 28803482 |