| biphasic effect of pertussis vaccine on serum insulin in mice. | administration of pertussis vaccine, consisting of whole, killed bordetella pertussis organisms, causes hyperinsulinemia and enhanced secretion of insulin in response to a variety of secretagogues in rats and mice. in examining the time course and properties of this phenomenon, we discovered two distinct and separate effects of the bacteria on glucose and insulin levels in mice. first, a heat-stable (80 degrees c for 30 min) component causes a brief hyperinsulinemia which is +measureable by 1 h, ... | 1983 | 6345388 |
| sensitivity of passive cutaneous anaphylaxis in rats. i. inverse relationship between pca sensitivity and amount of ige present on mast cells. | passive cutaneous anaphylaxis (pca) sensitivity and ige antibody production were examined in rats from four different outbred colonies: wistar a, wistar b, sprague-dawley, and donryu. pca sensitivity was the highest in wistar b and the lowest in wistar a rats using anti-ascaris, antibovine serum albumin, and anti-clonorchis sinensis ige antibodies. the pca sensitivity related inversely to the reverse pca reaction with rabbit antirat ige antibody, suggesting that preexisting ige molecules on the ... | 1983 | 6347905 |
| prolongation of skin graft survival across different genetic barriers in rats with cyclosporine--and its potentiation by bordetella pertussis vaccine. | skin allografting was performed in rats treated with cyclosporine using strain combinations that differed across the rt1.a (class i) or rt1.b (class ii) loci of the major histocompatibility complex (mhc) or across non-mhc loci. injection of cyclosporine (20 mg/kg) for 14 consecutive days was followed by prolonged acceptance (mst = 67 days) of the skin allografts. bordetella pertussis vaccine potentiated the effect of cyclosporine, and the synergistic effect of the vaccine occurred only when it w ... | 1983 | 6349041 |
| effects of pertussis toxin treatment on the metabolism of rat adipocytes. | the protein toxin present in bordetella pertussis vaccine blocks the inhibition of adenylate cyclase by prostaglandins and adenosine which may be secondary to adp-ribosylation of an inhibitory guanine nucleotide-binding protein. the stimulatory effects of alpha 1-catecholamine agonists on 32p uptake into phosphatidic acid and phosphatidylinositol in isolated rat adipocytes were virtually abolished by pertussis toxin treatment. in contrast, the stimulatory effects of insulin were increased in adi ... | 1983 | 6350297 |
| modulation of the biologic activities of ige-binding factor. v. the role of glycosylation-enhancing factor and glycosylation-inhibiting factor in determining the nature of ige-binding factors. | glycosylation-enhancing factor (gef) and ige-potentiating factor were detected in culture supernatants of rat mesenteric lymph nodes (mln) cells obtained 14 days after infection with nippostrongylus brasiliensis (nb), but not in supernatants of mln cells of 8-day nb-infected rats. both factors were also released from t cells upon antigenic stimulation of klh + alum-primed spleen cells. the gef from the nb-infected rats and klh + alum-primed spleen cells had affinity for p-aminobenzamidine agaros ... | 1984 | 6363537 |
| effects of islet-activating protein on insulin- and isoprenaline-stimulated glucose transport in isolated rat adipocytes. | the effects of islet-activating protein (iap), a bordetella pertussis toxin, on insulin- and isoprenaline-stimulated glucose transport were studied in isolated rat adipocytes. basal as well as insulin-stimulated glucose transport were not affected when cells were pretreated with iap. in contrast, iap pretreatment abolished the stimulatory effect of isoprenaline. when iap-pretreated cells were exposed to a combination of insulin and isoprenaline, the catecholamine significantly reduced the stimul ... | 1984 | 6365589 |
| the regulation of tissue eosinophil chemotactic factor and inhibitor in allergic skin lesions of freund's complete adjuvant-treated guinea-pigs. | the regulation of tissue eosinophilia induced by dinitrophenyl-ascaris extract (dnp-as) was investigated in guinea-pigs. biphasic tissue eosinophilia peaking at 6 and 24 hr was observed in the skin lesions in bordetella pertussis vaccine (bp)-treated animals. in contrast, only the early phase of tissue eosinophilia was observed in freund's complete adjuvant (fca)-treated animals. although less eosinophil chemotactic activity was detected in 24-hr-old skin extract of fca-treated animals (fca-extr ... | 1984 | 6365742 |
| the effect of pertussis vaccine on the insulin-dependent diabetes induced by streptozotocin in mice. | protective effect of pertussis vaccine (pv) against the development of insulin-dependent diabetes (idd) induced by streptozotocin (stz) in young cd-1 male mice were studied. when pv at a dose of 1.2 x 10(10) microorganism was administered on -10, 0, or +4 d relative to a single injection of stz (60 mg/kg body wt) on d 0, it completely aborted the development of idd after 140 d. when pv was given on +30 d after stz injection and followed by pv booster injections, 66% of mice reverted to normoglyc ... | 1984 | 6366724 |
| islet-activating protein (iap)-induced adrenergic modulation of pancreatic a and b cell in dogs. | islet-activating protein (iap) is a substance purified from the culture medium of bordetella pertussis, and its main action is characterized by the enhancement of secretory response to glucose and other stimuli in pancreatic islet. in this experiment, the effect of iap on epinephrine-induced secretion of immunoreactive insulin (iri) and glucagon (irg) was investigated in normal dogs. epinephrine suppressed iri secretion and it had a little increment to irg secretion in control group, while iri a ... | 1984 | 6370819 |
| bordetella pertussis whole cell vaccines--efficacy and toxicity. | the literature concerning efficacy and side effects of pertussis vaccines is reviewed. with few exceptions, most vaccines induce a protective immunity lasting for 2 to 5 years. the large-scale use of pertussis vaccines has markedly contributed to the decrease in pertussis morbidity in small children but in some countries the incidence has increased in older children. not even countries with immunisation rates of 90-95% have managed to eradicate pertussis or prevent disease in infants below the a ... | 1984 | 6380211 |
| comparative effect of selected adjuvants on the response in the bovine mammary gland to staphylococcal and streptococcal antigens. | the effect of 4 adjuvants on the response in the lactating bovine mammary gland to an antigenic stimulus was examined. fifty four lactating holstein friesian cows were randomly allocated to 6 groups. four of these groups received a staphylococcal and streptococcal bacterin-toxoid vaccine administered systemically in association with an adjuvant preparation. the adjuvants used were: aluminum hydroxide gel, freund's incomplete adjuvant, a metabolizable lipid emulsion and bordetella pertussis. two ... | 1984 | 6385464 |
| effect of bordetella pertussis toxin on adp-ribosylation of membrane proteins, adenylate cyclase activity and insulin release in rat pancreatic islets. | exposure of rat pancreatic islet membranes to [alpha-32p]-nad+ in the presence of bordetella pertussis toxin (islet-activating protein) reveals the adp-ribosylation of a peptide with a mr close to 41 kda, which corresponds to the alpha-subunit of the guanine nucleotide regulatory protein ni. islets removed from rats pretreated with the bordetella pertussis toxin display a specific increase in adenylate cyclase responsiveness to gtp and are characterized by a resistance to the inhibitory action o ... | 1984 | 6388572 |
| isolation of two proteins with high affinity for guanine nucleotides from membranes of bovine brain. | membranes from bovine brain bind relatively large quantities of guanosine 5'-(3-o-thio)triphosphate (gtp gamma s) with high affinity. the two proteins responsible for most of this activity were purified; they account for 1.5% of the membrane protein. the two proteins contain alpha subunits of either 39,000 or 41,000 da, beta subunits of 36,000 or 35,000 da, and a potential gamma subunit (11,000 da). these structures are the same as a family of proteins that includes transducin and the regulatory ... | 1984 | 6438083 |
| does the guanine nucleotide regulatory protein ni mediate progesterone inhibition of xenopus oocyte adenylate cyclase? | in xenopus laevis oocytes progesterone is able to inhibit directly the plasma membrane adenylate cyclase activity and induce reinitiation of meiotic maturation. to determine whether progesterone inhibition is mediated by the inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase, ni, the effect of the bordetella pertussis toxin (iap) and limited proteolysis on progesterone action in oocytes was investigated. treatment of oocyte membranes with islet activating protein (ia ... | 1984 | 6439557 |
| high admission rate of infants and young children with whooping cough: clinical aspects and preventive implications. | eighty-five children hospitalized with clinical whooping cough over an 8 month period were studied. of the 53 cultured for bordetella pertussis, 29 (55%) were culture positive. there was a preponderance of young infants who required a long hospitalization (35% were less than 3 months of age). many of these children had apnoeic attacks requiring active intervention. pulmonary atelectasis-consolidation was found in 46% in spite of negative viral and bacterial studies. over-crowding and poor socioe ... | 1984 | 6466223 |
| [effect of cucumarioside (a triterpene glycoside from the holothurian cucumaria japonica) on the development of an immune response in mice to corpuscular pertussis vaccine]. | the influence of cucumarioside, triterpene glycoside obtained from cucumaria japonica (echinodermata, holoturioidea), or sea cucumbers, on the resistance of mice to bordetella pertussis infection (with the use experimental pertussis meningoencephalitis as a model) and on the development of immune response to corpuscular pertussis vaccine was studied. the preparation under test was shown to have greatly pronounced immunomodulating properties depending on both the concentration of cucumarioside an ... | 1984 | 6506936 |
| molecular mechanisms of lymphocyte extravasation. i. studies of two selective inhibitors of lymphocyte recirculation. | pertussigen, a protein toxin purified from bordetella pertussis, and fucoidin, a high molecular weight sulfated polysaccharide, were analyzed for their ability to inhibit lymphocyte recirculation in vivo. pertussigen treatment of lymphocytes resulted in a dosage- and time-dependent loss of their ability to localize in lymph nodes or peyer's patches. this toxin-induced alteration did not reverse after extended lymphocyte culture in toxin-free media, and had no effect on lymphocyte viability or ac ... | 1984 | 6537815 |
| effect of pertussigen on inflammation caused by freund adjuvant. | pertussigen, one of the toxins from bordetella pertussis, greatly increased the inflammatory response produced by complete freund adjuvant in the footpads of mice. this effect was not produced by pertussigen when the emulsion was made with saline and incomplete freund adjuvant, but if an antigen was included in incomplete freund adjuvant, the strong potentiating effect was again demonstrated. as little as 100 ng of pertussigen given intravenously was effective, but 400 ng proved better, and this ... | 1984 | 6539301 |
| progesterone inhibition of xenopus oocyte adenylate cyclase is not mediated via the bordetella pertussis toxin substrate. | preincubation of intact xenopus laevis oocytes with islet-activating protein (iap) stimulates plasma membrane adenylate cyclase activity measured in the presence of either gtp or 5'-guanylyl imidodiphosphate. the toxin appears to act by catalyzing the adp-ribosylation of a 41,000-d protein in the oocyte plasma membrane that is distinct from the 45,000- and 52,000-d proteins labeled by cholera toxin. preincubation of intact oocytes with iap for 2.5 hr inhibits 80% of the adp-ribosylation of oocyt ... | 1984 | 6541752 |
| bordetella pertussis infection in mice: correlation of specific antibodies against two antigens, pertussis toxin, and filamentous hemagglutinin with mouse protectivity in an intracerebral or aerosol challenge system. | the correlation of titers of specific serum immunoglobulin g antibodies against two antigens, pertussis toxin (pt), and filamentous hemagglutinin (fha), which are the main components of pertussis vaccine in japan, with mouse protectivity was examined by both intracerebral and aerosol challenge systems with virulent bordetella pertussis cells. titer of the antibodies was calculated from the enzyme-linked immunosorbent assay (elisa) unit given arbitrarily to reference antibodies. pt antibody titer ... | 1984 | 6542069 |
| modulation of the biologic activities of ige-binding factor. iv. identification of glycosylation-enhancing factor as a kallikrein-like enzyme. | stimulation of normal rat splenic t cells with pertussigen (lymphocytosis-promoting factor, lpf, from bordetella pertussis) resulted in the release of a soluble factor that enhanced the glycosylation of ige-binding factors during their biosynthesis. the soluble factor was detected by the ability of a culture filtrate of lpf-stimulated spleen cells to switch a t cell hybridoma, 23a4, from the formation of unglycosylated ige-binding factor to the formation of glycosylated ige-binding factor. the g ... | 1983 | 6555215 |
| modulation of the biologic activities of ige-binding factor. ii. physicochemical properties and cell sources of glycosylation-enhancing factor. | t lymphocytes of rats treated with bordetella pertussis vaccine (bp) formed a soluble factor that enhanced the glycosylation of ige-binding factors during their biosynthesis, and provided the latter factors with the biologic activity to potentiate the ige response. the present experiments demonstrated that pertussigen (leukocytosis-promoting factor) from bp induced normal rat spleen cells to form the glycosylation-enhancing factor. the same factor was obtained by incubation of normal spleen cell ... | 1983 | 6601139 |
| the correlation between the activation state of b cells and their capacity for in vitro propagation of immunologic memory. | the b-cell population responsible for in vitro antigen-mediated proliferation and expansion of the memory b-cell population is a large activated blast. such cells predominate early after antigen priming and can be regenerated by adjuvant (bordetella pertussis) stimulation in vivo. although these cells are proliferating in vivo, additional stimuli are needed for expansion of the memory population in vitro. these triggering requirements include specific antigen (dnp-ova) and the assistance of adhe ... | 1983 | 6601515 |
| elicitation of experimental allergic encephalomyelitis (eae) in mice with the aid of pertussigen. | pertussigen, one of the biologically active proteins from bordetella pertussis, was found highly active as an adjuvant to promote the induction of experimental allergic encephalomyelitis (eae) in (sjl x balb/c)f1 mice that had received at the same time an injection of mouse spinal cord (msc) homogenized in complete freund's adjuvant containing 4 mg of mycobacterium tuberculosis h37ra per milliliter (cfa-h37). in this system 2 mg of msc induced eae, but a dose of 4 mg was more effective. as littl ... | 1984 | 6607126 |
| physiology of igd. iii. effect of treatment with anti-igd from birth on the magnitude and isotype distribution of the immune response in the spleen. | continued treatment with monoclonal anti-igd (ig-5a) from birth in balb/c mice causes a markedly increased responsiveness to i.v. injected dinitrophenylated ovalbumin (dnp-ova) with bordetella pertussis at the age of 8 weeks. the 19s plaque-forming cell (pfc)/spleen response is particularly enhanced, 6-8-fold, but all the other isotypes also show increases of 2-6-fold, including iga and ige. both primary and secondary pfc responses and serum antibody titers are enhanced. after transfer of spleen ... | 1984 | 6607169 |
| proliferative response of immune mouse t-lymphocytes to the lymphocytosis-promoting factor of bordetella pertussis. | immunization of mice with a whole-cell pertussis vaccine or with the purified, detoxified lymphocytosis-promoting factor (lpf) of bordetella pertussis resulted in an increased in vitro proliferative response to lpf in immune lymph node cells. the proliferative response was detected above the nonspecific mitogenic activity of lpf. that the proliferative response of the immune lymph node cells was a demonstration of a specific cell-mediated immunity to lpf was supported by the following: (i) the s ... | 1984 | 6608495 |
| involvement of the pineal gland in rats with experimental autoimmune uveitis. | pineal glands of rats with experimentally induced autoimmune uveitis (eau) were studied histologically. inflammatory changes, characterized by mononuclear infiltration, were found in the pineal glands of one-third of the lewis rats that developed eau by active immunization with s-antigen. no changes in the pineal gland were observed in avn rats which are "low responders" for eau and did not develop ocular disease. frequency and severity of both pineal gland and ocular involvement clearly were el ... | 1983 | 6618794 |
| investigations into the induction of chronic experimental arthritis in the common marmoset (callithrix jacchus). | in an attempt to produce a superior model of rheumatoid arthritis, experiments have been performed to investigate the ease of induction of experimental arthritis in marmosets by immunological means. marmosets were sensitised with the following combinations of antigen and adjuvant: ovalbumin in freund's complete adjuvant (fca), ovalbumin in fca + bordetella pertussis, methylated-bsa in fca + b. pertussis or human fibrin in fca + b. pertussis, and subsequently injected with the corresponding antig ... | 1983 | 6622924 |
| attempts to modify lung granulomatous responses to schistosoma japonicum eggs in low and high responder mouse strains. | a radioisotopic assay for acute granulomatous hypersensitivity (agh) to lyophilized eggs of schistosoma japonicum has been used to further examine responses to egg antigens in various inbred strains of mice. the ranking of responsiveness in mice from high (c57bl/6), intermediate (balb/c) to low (cba/h) was not influenced by high or low egg-sensitization regimens. however, the low responsiveness of responder mice sensitized with eggs by the intraperitoneal compared with the subcutaneous route of ... | 1983 | 6651645 |
| [susceptibility of clinical isolates in pediatrics to cefpiramide]. | cefpiramide (cpm, sm-1652) had broad-spectrum antibacterial activities against most of clinically isolated organisms to which are paid attention as pathogenic organism in the field of pediatrics. antibacterial activities of cpm against staphylococcus aureus, streptococcus pyogenes, haemophilus influenzae, bordetella pertussis and proteus mirabilis were almost the same as those of cefoperazone (cpz). antibacterial activities of cpm against escherichia coli and klebsiella pneumoniae were somewhat ... | 1983 | 6655831 |
| the course of lymphocytic choriomeningitis virus infection in germfree mice treated with bordetella pertussis vaccine. | a single injection of bordetella pertussis vaccine, applied intraperitoneally one day before intracerebral lymphocytic choriomeningitis virus infection depressed the immune response both in conventional and germfree adult mice, but the rate of the immunosuppressive effect differed. in adult mice with a normal immune system the vaccine only delayed the manifestation of fatal lymphocytic choriomeningitis, while it prevented its development in germfree mice with an underdeveloped lymphoid system, i ... | 1983 | 6659851 |
| the kinetics of in vivo sensitization of rat peritoneal and lung mast cells: temporal dissociation from circulating levels of ige. | following i.p. injection of ovalbumin (ova) plus bordetella pertussis vaccine into hooded lister rats, the time-course of sensitization of peritoneal and lung mast cells (mc) did not parallel kinetic changes in the levels of circulating ova-specific and total ige. ova-induced secretion of 5-hydroxytryptamine from isolated peritoneal and lung mc and the presence of ova-specific ige in serum were first demonstrated at day 14 post-immunization. however, subsequent to day 14, the responsiveness of b ... | 1983 | 6662189 |
| bacterial modulation of the cellular immune response in mice. i. the course of lymphocytic choriomeningitis virus infection in bordetella pertussis vaccine pretreated mice with physiological thymus involution. | the cellular immune response to lymphocytic choriomeningitis virus infection was found to be normal in 6-month-old mice with physiological thymus involution, while it was reduced in 18-month-old mice. the bordetella pertussis vaccine elicited immunosuppression in 6-month-old mice with normal immunological responsiveness, while it failed to affect the physiologically diminished cellular immune response in 18-month-old mice. the extent of immunosuppression elicited by the vaccine changed parallel ... | 1983 | 6675411 |
| pertussis toxin uncouples dopamine agonist inhibition of prolactin release. | pertussis toxin, a protein exotoxin produced by bordetella pertussis, markedly reduced or eliminated the ability of dopamine or the dopamine agonist bromocriptine to inhibit prolactin release from anterior pituitary cells in vitro. toxin-mediated reversal of the effect on dopamine agonist inhibition of prolactin release occurred with a lag of greater than 6 h, was maximal by 24 h, and persisted for at least 6 days after removal of the toxin from the medium. the toxin reduced dopamine agonist eff ... | 1983 | 6682634 |
| immunopathology of murine experimental allergic orchitis. | experimental allergic orchitis (eao) was induced consistently in balb/c mice by immunization with homologous testicular tissue homogenate emulsified in complete freund's adjuvant (cfa) providing that the animals had received simultaneously at least 1 microgram of an extract of bordetella pertussis rich in pertussigen. all animals thus treated developed orchitis and serum antibody to testicular antigens within 20 days after immunization. the lesions were located in testis (100%), rete testis (37% ... | 1983 | 6682874 |
| effect of bordetella pertussis leukocytosis (lymphocytosis)-promoting factor (lpf) on the physical lymphoepithelial-cell association studied with the use of an in vitro model of mouse thymus. | the effect of highly purified leukocytosis (lymphocytosis)-promoting factor (lpf) of bordetella pertussis on physical lymphocyte and reticuloepithelial (re) cell association was studied in an in vitro thymus model. first, a simplified in vitro system to assess the lympho-re-cell association was developed. a completely confluent layer of thymic re cells was formed by cultivating trypsinized thymus cell suspensions from 2- to 7-day-old mice. when thymic lymphoid cells were seeded on this cell laye ... | 1983 | 6682875 |
| grass conjuvac. ii. pro-inflammatory activity and potential for inducing hypersensitivity. | treatment of rats with conjuvac induced no anti-pollen extract (pe) ige and no sensitization, whereas alum-adsorbed pollen extract induced ige antibody and marked sensitivity. conjuvac induced anti-pe ige in rats treated with bordetella pertussis organisms but the antibody concentrations were less than those induced by pe with b. pertussis. there was no indication, either in rats injected with b. pertussis and conjuvac or alginate (alg), of sensitization to alg. in guinea pigs, conjuvac induced ... | 1984 | 6693179 |
| lotifazole (f 1686), a non-steroidal anti-inflammatory agent with an unusual pharmacological spectrum. | lotifazole (f 1686) - 4-phenyl-2-(2',2',2-trichloroethoxycarboxamido) thiazole - has a range of anti-inflammatory activities in animals that differs from the activities of classic non-steroidal drugs. it reduces carrageenin-induced oedema in rats, uv-induced erythema in guinea pigs, and arthus pleurisies in rats only at high doses. it does not affect freund's-adjuvant polyarthritis, and it only slightly affects passive skin anaphylaxis in rats and anaphylactic shock in guinea pigs. lotifazole do ... | 1984 | 6702514 |
| islet-activating protein (iap) reduces bethanechol-stimulated release of pancreatic polypeptide in the dog. | the effect of islet-activating protein (iap) purified from culture medium of bordetella pertussis was examined in dogs. this was assessed by the levels of pancreatic polypeptide (pp) as well as the responses of plasma insulin and glucagon to a parasympathomimetic agent, bethanechol. plasma responses of these pancreatic hormones were measured before and 5 days after iap injection. although iap had no significant effect on the bethanechol-stimulated increase in plasma glucose, insulin and glucagon ... | 1982 | 6756897 |
| [topics on bordetella pertussis vaccine]. | | 1982 | 6757473 |
| inhibition of murine reaginic antibody responses by nasal immunotherapy with modified allergen. | optimal conditions were established for induction of reaginic antibodies to lolium perenne pollen allergens in mice by intranasal dosing of allergens with bordetella pertussis vaccine. this antibody response could be inhibited by pretreatment of the mice by nasal administration of 100 microgram of glutaraldehyde-modified l. perenne allergens 9 times in 3 weeks before priming, whereas native allergens, in doses of 5 microgram, did not inhibit an ige response to subsequent priming. it was not poss ... | 1980 | 6769824 |
| serotype determinant protein of neisseria meningitidis. large scale preparation by direct detergent treatment of the bacterial cells. | neisseria meningitidis group b microorganisms, inactivated with phenol and harvested by centrifugation, were subjected to direct treatment with various detergents to solubilize the serotype determinant proteins localized in the outer membrane. analysis of the data showed that extraction of the cells with detergents provided yields of the serotype protein substantially exceeding those obtained by simple salt extraction of the bacteria. routinely, more than 2 mg of end product per g of cell mass ( ... | 1981 | 6794337 |
| [experimental allergic encephalomyelitis in guinea pigs caused by meningococci]. | the sensitization of guinea-pigs with the mixture of meningococci and heterologous-cerebral tissue commonly induces the development of the typical clinical and pathomorphological picture of allergic encephalomyelitis. unlike mycobacterium tuberculosis and other acid-resistant bacterial and much like bordetella pertussis, meningococci are capable of inducing allergic encephalomyelitis when introduced in mixture with oil without cerebral tissue. the vaccinal strain induces allergic encephalomyelit ... | 1981 | 6800162 |
| acute autoimmune encephalomyelitis in mice. ii. susceptibility is controlled by the combination of h-2 and histamine sensitization genes. | the expression of acute experimental autoimmune encephalomyelitis (eae) in mice is controlled by several dominant genes, h-2 and histamine sensitization genes. sjl/j and swr/j, which are h-2s and h-2q, respectively, are susceptible to eae and sensitive to bordetella pertussis histamine-sensitizing factor (hsf), which produces a vasoactive amine hypersensitivity. other h-2s or h-2q strains such as a.sw, b10.q and several others do not develop acute eae and are not sensitive to b. pertussis hsf. o ... | 1982 | 6806429 |
| specific monoclonal igm is a potent adjuvant in murine malaria vaccination. | recent experiments in the murine system have indicated that the passive acquisition by offspring of maternal anti-malarial igg antibodies while conferring some degree of immunity against a primary infection, paradoxically prevents the generation of acquired immunity through vaccination. therefore, in view of earlier findings concerning the competitive effects of specific igm and igg antibodies, we investigated whether specific monoclonal igm antibodies could be used to potentiate the response to ... | 1983 | 6835362 |
| susceptibility of bordetella species to growth inhibition and killing by chlorpromazine. | chlorpromazine, the prototype phenothiazine tranquilizer, inhibited the growth and killed organisms of the genus bordetella. there were striking differences, however, among the three bordetella species. bordetella pertussis was most susceptible, with some inhibition of growth at greater than or equal to 4 micrograms/ml and killing at 16 micrograms of chlorpromazine per ml. bordetella parapertussis and bordetella bronchiseptica were less susceptible, with killing at 32 and 256 micrograms/ml, resp ... | 1983 | 6838185 |
| agglutinin directed against bordetella pertussis in a chicago population. | | 1983 | 6842030 |
| scanning electron microscopy of mouse ciliated oviduct and tracheal epithelium infected in vitro with bordetella pertussis. | infection of mouse tracheal organ culture with bordetella pertussis resulted in ciliostasis within 36 h. scanning electron microscopy revealed that b. pertussis attached exclusively to ciliated cells but did not induce expulsion of this cell type at a test interval of 48 h. mouse oviduct organ culture infected with b. pertussis demonstrated the same strict tropism for ciliated cells as in the tracheal ring system. only ciliated cells were parasitized, becoming heavily colonized 48 h postinfectio ... | 1983 | 6850422 |
| the non-specific enhancement of allergy. i. in vivo effects of bordetella pertussis vaccine on ige synthesis. | bordetella pertussis organisms, with or without a small dose of alumhydroxide, enhance in rats the production of ige antibodies to an unrelated antigen, even if this antigen has been administered 6 weeks beforehand. this non-specific enhancement of ige antibodies is accompanied by a substantial rise in total serum ige and by the production of ige antibodies to b. pertussis. the effect of b. pertussis on ige synthesis is dose-dependent. no effect of b. pertussis on ige production can be observed ... | 1983 | 6869702 |
| acute experimental autoimmune encephalomyelitis in mice. i. adjuvant action of bordetella pertussis is due to vasoactive amine sensitization and increased vascular permeability of the central nervous system. | | 1982 | 6891621 |
| regulatory role of ige-binding factors from rat t lymphocytes. v. formation of ige-potentiating factor by t lymphocytes from rats treated with bordetella pertussis vaccine. | an i.p. injection of bordetella pertussis vaccine (bp) into rats induced the formation of soluble factors that had affinity for ige (ige-binding factors). the factor was detected in the serum of bp-treated animals 5 to 7 days after the treatment. their circulating lymphocytes as well as spleen cells spontaneously released ige-binding factors in the serum of bp-treated rats and those released from their circulating lymphocytes had affinity for lentil lectin, and the ability to selectively potenti ... | 1981 | 6970222 |
| helper activity of t lymphocytes which have been stimulated by keyhole limpet haemocyanin in vitro. | details are given of a system for keyhole limpet haemocyanin (klh)-induced dna synthesis by murine t lymphocytes in vitro. lymph node t cells from mice primed with klh and bordetella pertussis were stimulated with klh under the defined conditions, and it was found that such cultured cells exhibited substantial non-specific helper activity. in contrast similarly primed t cells which had not been cultured showed only antigen-specific help. it is concluded that proper account should be taken of non ... | 1981 | 6972901 |
| depression of hepatic mixed-function oxidase activity by b. pertussis in splenectomized and athymic nude mice. | administration of bordetella pertussis (b. pertussis), corynebacterium parvum (c. parvum), and several other immunoactive substances is known to cause a marked decrease in the activity of the hepatic microsomal mixed-function oxidase system. the effect of c parvum has been reported to involve the reticuloendothelial system. in the present study, the effect of b. pertussis administration to decrease hepatic microsomal drug metabolism was studied in unoperated, sham-operated, and splenectomized mi ... | 1981 | 6972935 |
| role of t cells in the development of memory b cells. quantitative and qualitative analysis. | the purpose of this investigation was to address the current controversy regarding the t-cell requirement for the generation of b-memory cells. we have circumvented the possible objection to previous experiments regarding residual t cells in t-deprived animals by examining memory cell generation in relation to the numbers of t cells participating in the immune response. thymectomized and lethally-irradiated rats were reconstituted with foetal liver or a more mature stem cell source, neonatal liv ... | 1981 | 6974133 |
| formation of ige-binding factors by rat t lymphocytes. ii. mechanisms of selective formation of ige-potentiating factors by treatment with bordetella pertussis vaccine. | | 1981 | 6975297 |
| affinity, cross-reactivity and biological effectiveness of rabbit antibodies against a synthetic 37 amino acid c-terminal peptide of human chorionic gonadotrophin. | as a part of a programme to develop a fertility regulating vaccine, antibodies specific for human chorionic gonadotrophin were raised by immunizing rabbits with a synthetic 37 amino acid c-terminal peptide of beta hcg conjugated to tetanus toxoid as carrier, and using bordetella pertussis or freund's complete adjuvant as adjuvants. lack of cross-reactivity of the antibodies with human luteinizing hormone was determined by direct binding in a radioimmunoassay and by immunofluorescence on adult hu ... | 1980 | 6993062 |
| effect of bordetella pertussis vaccine on glucagon secretion in normal and alloxan dogs. | the effect of bordetella pertussis vaccine on plasma glucose, insulin and glucagon secretion was investigated in normal and alloxan dogs. on the 8th day after the vaccine injection, in normal and alloxan dogs during the infusion of arginine and glucose, the plasma glucose level was lower and the iri level was higher than in the saline controls. on the other hand, the plasma irg level showed no significant alloxan dogs this vaccine made the plasma irg level lower during arginine infusion than in ... | 1980 | 7016512 |
| the generation of memory cells. iv. immunization with antigen-antibody complexes accelerates the development of b-memory cells, the formation of germinal centres and the maturation of antibody affinity in the secondary response. | hapten (dnp)-specific b-memory cells were induced by priming mice with soluble or alum precipitated dnp-haemocyanin (klh) plus bordetella pertussis or cnp-klh-anti-dnp antibody complexes at equivalence. cells from mice given complexes gave a substantial adoptive igg response five days after priming, whereas those from mice given antigen with conventional adjuvant did not give a comparable response until day 14. soluble antigen induced poor memory, even 14 days after primary immunization. the eme ... | 1981 | 7019054 |
| bordetella pertussis-induced hyperinsulinaemia without marked hypoglycaemia: a paradox explained. | b. pertussis vaccine or pulmonary infection produced marked hyperinsulinaemia in mice relative to controls (e.g. control 32 mu/1; b. pertussis infected 113 mu/1). this was associated with a modest relative hypoglycaemia (15-25%). the hyperinsulinaemia was observed only when blood was collected from mice anaesthetized with ether, pentobarbitone, or trichloroethylene but not from unanaesthetized animals. ether-induced hyperinsulinaemia in b. pertussis was transient. adrenaline produced marked hype ... | 1981 | 7028074 |
| adjuvant effect of vitamin a palmitate and analogs on cell-mediated immunity. | the sc route was used to study the adjuvant effect of vitamin a palmitate (va) and the retinoids isotretinoin (ro 4-3780), etretinate (ro 10-9359), and motretinide (ro 11-1430) on cell-mediated immunity to sheep red blood cells (srbc) in outbred mai:nih(s) mice. the immune response was quantified by a sensitive assay procedure in which the antigen is injected intracutaneously into the pinna of the mouse, and the subsequent inflammatory swelling is measured with calipers. a single simultaneous in ... | 1981 | 7029099 |
| effect of islet-activating protein (iap) upon insulin secretion from human pancreatic islets. | a new protein termed islet-activating protein (iap) has recently been extracted from the culture medium of bordetella pertussis, and shown to enhance insulin secretion in vivo in rats or in vitro in isolated pancreatic islets due to activation of native calcium ionophores. however, it has not been clarified whether or not iap enhances the secretion of insulin in human pancreatic islets. in order to examine the effect of iap on human pancreatic islets, pancreatic tissues were obtained from seven ... | 1981 | 7030721 |
| t cell responses to alloantigens. iv. in vivo and in vitro studies of the abrogation of presensitization to major histocompatibility antigens. | b6af1 mice were sensitized to major histocompatibility complex (mhc) antigens by rejection of b10.d2 skin allografts. the effects of various protocols of treatment with antithymocyte serum (ats), bordetella pertussis vaccine, and donor strain lymphocytes were tested in these mice, and the results were assessed by in vitro testing for abrogation of circulating cytotoxic t cell memory and in vivo by determining the survival time of a second donor strain skin graft. it was found that a 6-week cours ... | 1982 | 7041359 |
| ige and haemagglutinating antibody production in mice following intranasal immunization with ryegrass pollen. | repeated intranasal immunization of mice whole ryegrass pollen (rgp) led to the development of high circulating titres of both ige and haemagglutinating antibodies. bordetella pertussis vaccine (bpv) was required at the time of initial immunization, although its repeated use did not affect ige production. antibody production was maintained at high levels for greater than 4 months. significant differences were found in the ability of different batches and strains of bpv to induce production. | 1982 | 7056597 |
| chronologic neuropathology of relapsing experimental allergic encephalomyelitis in the mouse. | the neuropathology has been described of chronic, relapsing experimental allergic encephalomyelitis produced in sjl/j mice given two injections of isogeneic spinal cord in complete freund's adjuvant, 1 week apart. the inducing inoculum contained no bordetella pertussis. the central nervous system changes included hemorrhagic lesions and significant nerve fiber depletion during the early stages of disease, demyelination followed by remyelination, influxes of polymorphonuclear leukocytes and hemor ... | 1982 | 7062721 |
| adults with pertussis. | eighty adults were diagnosed in one general practice as having infection due to bordetella pertussis, type 1.3, during a period of 30 months. their clinical presentation and progress is recorded. a plea is made for attention to be paid to this infection in adults. | 1982 | 7108855 |
| [action of bordetella pertussis vaccine on mouse hematopoietic stem cells]. | intravenous inoculation of killed bordetella pertussis vaccine into balb/c, cba, c57bl/6 mice or (cba x c57bl/6) f2 hybrids 1 day or 3 days before sublethal irradiation (5.5 gy) was shown to sharply increase the endogenous colony formation in the spleen 9 days after irradiation. moreover, the cfus content of the spleen and bone marrow was also enhanced 1 and 3 days after vaccination of the mice with 10(10) cells b. pertussis as revealed by the exocolonization technique (till and mcculloch). thus ... | 1982 | 7115919 |
| a comparison of saponin with other adjuvants for the potentiation of protective immunity by a killed plasmodium yoelii vaccine in the mouse. | the protective immunity conferred by subcutaneous injection of outbred cd-1 mice with a killed plasmodium yoelii (ym strain) vaccine was strongly potentiated by saponin. by adjusting the dose of antigen, the number of immunizations and the number of living parasites in the challenge infection, conditions were defined where antigen alone was non-protective but 100% protection was obtained by the addition of saponin. inbred balb/c, cba/ca and c57 b1 mice were much less responsive than the cd-1 mic ... | 1982 | 7145465 |
| severe impairment of cholinergic and adrenergic responsiveness in bordetella pertussis vaccinated rats. | | 1982 | 7160441 |
| [conversion of bordetella parapertussis serovar through lysogeny produced by pertussis phages]. | bacteriophages from bordetella pertussis were titrated on the indicator strain b. parapertussis 17903 by using standard soft agar techniques. secondary growth, occasionally observed in some phage plaques, was isolated and transferred onto selective media. judging from growth on these special media and microscopic examination the isolated clones consisted entirely of bordetellae. determination of the agglutinogen pattern of 160 of these clones revealed that 88% contained agglutinogen 1; 87.5% agg ... | 1982 | 7180238 |
| inhibition of mononuclear phagocyte elongation, migration, and cellular exudate formation following bordetella pertussis vaccine administration. | | 1981 | 7208486 |
| ige antibodies for penicillins and cephalosporins in rats. i. characteristics of the ige antibodies for penicillins and cephalosporins in rats. | rats immunized with sulbenicillin-ovalbumin (sbpc-ova) in combination with aluminum hydroxide (alum) and thimerosal-killed bordetella pertussis produced high levels of anti-sbpc antibodies. anti-sbpc antibodies were first detected on day 8, reaching the maximum titer on day 12 and rapidly declined thereafter. anti-sbpc sera obtained on day 13 were sulfhydryl-labile and heat-labile. the optimal latent period in the passive cutaneous anaphylaxis (pca) reaction was 60 approximately 72 hours. these ... | 1981 | 7251512 |
| effect of bordetella pertussis vaccine on neonatally thymectomized mice. | | 1981 | 7257878 |
| methods of quantitative microbiological analyses that support the diagnosis, treatment, and prognosis of human infection. | the microorganisms responsible for the production of an infection may be considered to be in two classes: classical microbes and host-defined microbes. classical microbes are those pathogens which fulfill the koch-henle postulates, and their isolation from a host indicates infection. they are not normally part of the body's normal flora, although they may be acquired by the host and enter into a passive relationship known as the carrier state. examples of this type of microbe are bacillus anthra ... | 1981 | 7273838 |
| whooping cough in relation to other childhood infections in 1977-9 in the united kingdom. | estimates based upon notifications indicate that there was in the 1977-9 triennium in the united kingdom the largest outbreak of whooping cough for 20 years or more. during this triennium there was also a sharp increase in other infections diseases of childhood, notably in non-notifiable respiratory infections. isolates of certain respiratory viruses ran in parallel and collectively outnumbered those of bordetella pertussis during the period of increase in notifications. there was highly signifi ... | 1981 | 7299339 |
| branhamella catarrhalis and other bacteria in the nasopharynx of children with longstanding cough. | nasopharyngeal cultures from 180 children aged 1 to 9 were examined. all children were suffering from cough for at least 10 days. the findings were compared to those from 67 non-coughing children. bordetella pertussis was isolated from 12.2% of the children in the study group but from none of the control children. branhamella catarrhalis was isolated from 66.1% of the children in the study group and from 28.3% in the control group (p less than 0.01). br. catarrhalis was more common in pure cultu ... | 1981 | 7313564 |
| formalin stress reaction of germfree and conventional mice treated with bordetella pertussis vaccine. | germfree and conventional mice responded similarly to pertussis vaccine treatment. in both groups, lymphocytosis and splenomegaly developed in a similar proportion. the formalin stress reaction of germfree and conventional mice with hypertrophic lymphoid organs induced by pertussis vaccine differed from that of untreated mice: the treated germfree and conventional mice showed a acute increase of lymphocytosis without an significant change in splenomegaly. | 1981 | 7315535 |
| [introduction to the section on bordetella pertussis]. | | 1981 | 7336285 |
| the lymphoid leukocytoses. | the experienced morphologist can be extremely helpful to the clinician by virtue of his or her ability to distinguish among the various subtypes of reactive lymphocytoses. an awareness on the part of the clinician as to the nuances of subclassification may lead to earlier diagnosis of a disease process. broadly, proliferations of normal lymphocytes point to infectious lymphocytosis or bordetella pertussis infection. proliferations of atypical lymphocytes, especially when minimum diagnostic crite ... | 1980 | 7352124 |
| immunotherapy of postoperative metastases of 13762a rat mammary adenocarcinoma: comparative effectiveness of bcg substrains and methods of preparation. | six bacillus calmette-guérin (bcg) substrains (phipps, pasteur, connaught, glaxo, riv, and tice) and two methods of preparation and storage (pellicle-lyophilized, dispersed-frozen and dispersed lyophilized) were directly compared in a standard immunotherapy protocol. complete freund's adjuvant and bordetella pertussis vaccine were also studied. all experiments included direct comparisons with corynebacterium parvum. the immunotherapy assay system was the 13762a rat mammary carcinoma in which imm ... | 1980 | 7397623 |
| neuropathology of experimental allergic encephalomyelitis in inbred strains of mice. | ten inbred strains of mice were tested for their susceptibility to experimental allergic encephalomyelitis (eae) after sensitization with mouse spinal cord in complete freund's adjuvant followed by booster injections of bordetella pertussis. the results extended previous findings in that not all susceptible mice possessed the h-2s haplotype, but mice with an h-2q background (dba1/j strain) were also susceptible. neuropathologic examination of experimental allergic encephalomyelitis in the mouse ... | 1980 | 7401630 |
| reduced tracheal mast cell numbers in adjuvant-sensitized rats associated with reduced pulmonary reactivity. | pvg/c inbred rats were sensitized by intra-peritoneal injection of dnp 19-ovalbumin with or without heat-killed bordetella pertussis as adjuvant. the use of adjuvant was associated with a greater serum ige, greater cutaneous immediate hypersensitivity, but no increase in immediate pulmonary reactivity to aerosol challenge. the number of mast cells in the trachea of adjuvant-sensitized rats was significantly reduced when compared with both unsensitized and allergen sensitized rats but this reduct ... | 1980 | 7429532 |
| the role of the liver in immunity to blood-stage murine malaria. | mice vaccinated with fixed parasitized red blood cells and bordetella pertussis can clear an otherwise lethal plasmodium yoelii infection in 7 days; this protection is abolished by splenectomy before vaccination. most mice splenectomized following vaccination were able to clear their infections, although their recovery was delayed. when labelled parasitized red cells were injected into mice during an infection, splenic uptake fell from day 3 onwards while uptake by the liver increased. lymphocyt ... | 1980 | 7439934 |
| inhaled bordetella pertussis vaccine decreases airway responsiveness in guinea pigs. | bordetella pertussis (bp) has been used as adjuvant for experimental animal immunization, but its effects on airway responsiveness are uncertain. three groups of guinea pigs were used: animals with a single exposure to inhaled bp vaccine (strain 134, total dose 1.24 x 10(12) germs), animals submitted to a sensitization procedure through inhalation of ovalbumin plus bp, and healthy control animals. four weeks after inhalation of bp or after the beginning of sensitization, dose- or concentration-r ... | 1995 | 7475913 |
| tracheal colonization factor: a bordetella pertussis secreted virulence determinant. | we report here the identification of a virulence-associated factor, tcf, (tracheal colonization factor), produced by strains of bordetella pertussis but not bordetella parapertussis or bordetella bronchiseptica. this protein is encoded by the tcfa gene. when a strain of b. pertussis 18323 lacking this protein is used to infect mice with an aerosol challenge, the number of bacteria isolated from the tracheas is decreased 10-fold when compared with the parent 18323. the derived amino acid sequence ... | 1995 | 7476158 |
| tracheal cytotoxin structural requirements for respiratory epithelial damage in pertussis. | the respiratory epithelial pathology of pertussis (whooping cough) can be reproduced by tracheal cytotoxin (tct), a disaccharide-tetrapeptide released by bordetella pertussis. tct is a muramyl peptide, a class of peptidoglycan-derived compounds which have many biological activities including adjuvanticity, somnogenicity, pyrogenicity, and cytotoxicity. the structural requirements for muramyl peptides to produce some of these biological effects have been partially characterized. using in vitro as ... | 1995 | 7476167 |
| sepa, the major extracellular protein of shigella flexneri: autonomous secretion and involvement in tissue invasion. | in addition to ipa proteins and icsa, which are involved in entry into epithelial cells and intercellular spread, respectively, shigella secretes a 110 kda protein, designated sepa. we report the identification, cloning, and nucleotide sequence determination of the sepa gene, analysis of sepa secretion, and construction and characterization of a sepa mutant. the sepa gene is carried by the virulence plasmid and codes for a 150 kda precursor. upon secretion, which does not involve accessory prote ... | 1995 | 7476198 |
| hybrid genes over-express pertactin from bordetella pertussis. | pertactin is a surface adhesin of bordetella pertussis which is produced in small quantities when expressed from the native prn promoter. hybrid genes were constructed in which the prn promoter was replaced by either the fha or tox promoter. recombinant b. pertussis strains containing chromosomally integrated hybrid tox promoter/prn (toxpprn) or fha promoter/prn (fhapprn) genes expressed pertactin at approximately 5- and 8-fold the wild-type level, respectively. the pertactin was correctly proce ... | 1995 | 7483778 |
| the purification and protective capacity of bordetella pertussis outer membrane proteins. | the whole cell vaccine (wcv) of bordetella pertussis is protective in the intracerebral (i.c.) mouse protection assay. we found a correlation between the i.c. mouse protection assay potency and the presence of the virulence-associated outer membrane proteins (omps) in outer membrane complexes (omc). the virulence-associated 92, 32 and 30 kda omps were purified and the n-terminal amino acid sequences were determined. the n-terminal amino acid sequences of the 30 and 32 kda omps show homology with ... | 1995 | 7483790 |
| [interaction between bordetella pertussis vaccine and chlorpromazine in mice]. | in acute toxicity experiments authors observed increased mortality on the first day following 75 mg/kg chlorpromazine (cpz) treatment in mice pretreated with bordetella pertussis vaccine (9 x 10(9) killed bacterium/mouse) compared to control animals treated with cpz alone. initially, the increased drug sensitivity observed after combined treatment was attributed to summation of the toxic effects. however, the cumulation of mortality did not cease on the following days; furthermore, an increase o ... | 1995 | 7484163 |
| an analogue of lipid a and lps from rhodobacter sphaeroides inhibits neutrophil responses to lps by blocking receptor recognition of lps and by depleting lps-binding protein in plasma. | when incubated with lipopolysaccharide (lps) in the presence of plasma, neutrophils become primed for enhanced release of superoxide in response to triggering by formyl-met-leu-phe (fmlp). the effect of lps on phagocytes is inhibited by a synthetic lipid a precursor, la-14-pp (lipid iva) or by lps from rhodobacter sphaeroides (rs). we studied the mechanisms by which la-14-pp or rs-lps inhibited lps-induced responses. when neutrophils were exposed to la-14-pp or rs-lps for 3 min and then to esche ... | 1995 | 7499965 |
| secretory iga against pertussis toxin and surface structures of bordetella pertussis in saliva of children with culture proven pertussis. | | 1995 | 7502896 |
| epithelial autotoxicity of nitric oxide: role in the respiratory cytopathology of pertussis. | bordetella pertussis releases a specific peptidoglycan fragment known as tracheal cytotoxin (tct) that reproduces the respiratory epithelial cytopathology of whooping cough (pertussis). in vitro, tct inhibits dna synthesis in hamster trachea epithelial cells and causes specific destruction of ciliated cells in explants of human and hamster respiratory epithelium. we have recently demonstrated that tct triggers production of intracellular interleukin 1 by respiratory epithelial cells, and this cy ... | 1994 | 7506415 |
| reduction of endotoxin contamination of various crude vaccine materials by gram-negative bacteria using aminated poly(gamma-methyl l-glutamate) spherical particles. | we describe a method for the removal of endotoxins from various crude antigen solutions originating from gram-negative bacteria using aminated poly(gamma-methyl l-glutamate) (pmlg) spherical particles. the aminated pmlg adsorbents showed high affinity for various purified endotoxins at an ionic strength of mu = 0.1. the endotoxin-adsorbing capacity of the adsorbent increased with increase in the amino-group content of the adsorbent. the adsorbent (3.2 meq/g amino-group content) showed the highes ... | 1993 | 7508778 |
| isotype and antigen specificity of pertussis agglutinins following whole-cell pertussis vaccination and infection with bordetella pertussis. | elevated agglutinin titers have been shown to correlate with protection from disease following whole-cell pertussis vaccination, but the isotype and antigen specificity of human agglutinating antibodies is unknown. in 13 immunoassays, immunoglobulin g antifimbria antibodies had the strongest correlation with agglutinin titers following culture-proven infection with bordetella pertussis (r' = 0.79; p < 0.0001) and following whole-cell pertussis vaccination (r' = 0.87, p < 0.0001). | 1994 | 7509316 |
| structural relationship between the s1 and s4 subunits of pertussis toxin. | pertussis toxin, the most important protective antigen of bordetella pertussis, is a 106-kda hexameric protein composed of an a-protomer (subunit s1) and a pentameric b-oligomer (s2 + s3 + 2s4 + s5). the most potent mouse-protective monoclonal antibodies against both respiratory and intracerebral infections were specified for either s1 or s4 and competed with each other in binding to epitopes of native pertussis toxin captured by haptoglobin or in solution, although they did not compete on unfol ... | 1994 | 7510256 |
| localization of antigenic domains on the major subunits of bordetella pertussis serotype 2 and 3 fimbriae. | antibody-binding domains on the major subunits of bordetella pertussis serotype 2 (fim2) and 3 fimbriae (fim3) have been identified using synthetic peptides which were screened for recognition by anti-protein monoclonal antibodies (mabs). the presence of non-contiguous fimbrial epitopes was demonstrated by both anti-fim2 and anti-fim3 mabs, several of which recognized at least two peptides that were discontinuous in the amino acid sequence of the corresponding subunits. the specificity of one ma ... | 1994 | 7512870 |
| formaldehyde treatment of proteins can constrain presentation to t cells by limiting antigen processing. | proteins to be used as vaccines are frequently treated with formaldehyde, although little is known about the effects of this treatment on protein antigenicity. to investigate the effect of formaldehyde treatment on antigen recognition by t cells, we compared the in vitro t-cell response to proteins that have been formaldehyde treated with the response to untreated proteins. we found that peripheral blood mononuclear cells from individuals vaccinated with three formaldehyde-treated proteins (pert ... | 1994 | 7513307 |
| effect of antibiotics on bordetella pertussis adhering activity: hypothesis regarding mechanism of action. | microbial adherence to epithelial cell surfaces has been implicated as the first step in the initiation of several infectious diseases. the ability of antibiotics to affect the properties of bacterial adherence to cell surfaces may be a criterion in selecting antibiotics for therapy. this study was performed in order to investigate the activity of amoxicillin, chloramphenicol, and clarithromycin in modifying the adhering activity of bordetella pertussis to human epithelial cells. the actions of ... | 1994 | 7515782 |
| filamentous hemagglutinin of bordetella pertussis. a bacterial adhesin formed as a 50-nm monomeric rigid rod based on a 19-residue repeat motif rich in beta strands and turns. | the filamentous hemagglutinin (fha) of bordetella pertussis is an adhesin that binds the bacteria to cells of the respiratory epithelium in whooping-cough infections. mature fha is a 220 kda secretory protein that is highly immunogenic and has been included in acellular vaccines. we have investigated its structure by combining electron microscopy and circular dichroism spectroscopy (cd) with computational analysis of its amino acid sequence. the fha molecule is 50 nm in length and has the shape ... | 1994 | 7519681 |
| modulation of hepatic mrna levels after administration of lipopolysaccharide and diphtheria and tetanus toxoids and pertussis vaccine adsorbed (dtp vaccine) to mice. | administration of whole-cell diphtheria and tetanus toxoids and pertussis vaccine adsorbed (dtp vaccine) caused marked depression in the expression of mrna for isozymes of cytochrome p-450 in the livers of endotoxin-responsive and nonresponsive mice. the levels of expression of mrna for a polycyclic aromatic hydrocarbon-inducible (cyp1a2) and an ethanol-inducible (cyp2e1) form of p-450 were reduced by 70% to 80% 8 to 12 hr after vaccination or bordetella pertussis endotoxin administration. these ... | 1994 | 7523268 |