| antigens/allergens of aspergillus fumigatus. identification of antigenic components reacting with both igg and ige antibodies of patients with allergic bronchopulmonary aspergillosis. | evidence that both ige and igg antibodies present in the sera of allergic bronchopulmonary aspergillosis patients (abpa) combine with several of the antigenic components of aspergillus fumigatus has been obtained using a self-crossed radioimmunoelectrophoresis test. in this test, patient's serum was used to develop its own crossed immunoprecipitation pattern to a reference antigen before proceeding to autoradiography with 125i-anti-human ige in the usual way. comparison of autoradiograph with im ... | 1983 | 6192954 |
| hyperreactivity of mediator-releasing cells from patients with allergic bronchopulmonary aspergillosis as evidenced by basophil histamine release. | histamine release was performed on 28 patients with allergic bronchopulmonary aspergillosis (abpa) in various stages and on 14 patients with mold-sensitive asthma, by using aspergillus antigens, other mold antigens, and anti-ige. total serum ige, ige and igg antibodies to aspergillus fumigatus (af), and end point cutaneous titration for af were determined for each patient. there was greater histamine release to aspergillus mix (p = 0.0000) and anti-ige (p = 0.047) in patients with abpa than in p ... | 1983 | 6194196 |
| development of fluorescent-antibody reagents for demonstration of pseudallescheria boydii in tissues. | we prepared fluorescent-antibody reagents to detect and identify pseudallescheria boydii in tissue. antisera to broken mycelium and condidia (particulate antigens) and to culture filtrates (soluble antigens) of p. boydii were produced in rabbits. antisera and globulin fractions of the antisera were labeled with fluorescein isothiocyanate and evaluated for their ability to stain p. boydii in tissues. the conjugates were first tested with cultures of 25 p. boydii isolates and 26 heterologous fungi ... | 1983 | 6195179 |
| immunosuppression in vitro by a metabolite of a human pathogenic fungus. | gliotoxin , a metabolite of aspergillus fumigatus, inhibits phagocytosis of macrophages at concentrations of 20-50 ng/ml. pretreatment of stimulator cells in mixed lymphocyte cultures with gliotoxin (100 ng/ml) abrogates induction of alloreactive cytotoxic t cells. the presence of gliotoxin 48 hr after initiation of cytotoxic t-cell induction has no effect. inhibition of cytotoxic t-cell induction by gliotoxin at low concentrations, acting on the stimulator cells, can be overridden by concanaval ... | 1984 | 6203127 |
| cavernoscopic evacuation of aspergilloma: an alternative method of palliation for haemoptysis in high risk patients. | | 1984 | 6204396 |
| protection of chickens by ribosomal vaccines from pasteurella multocida: dependence on homologous lipopolysaccharide. | chickens were protected against fowl cholera by ribosomal vaccines prepared from noncapsulated pasteurella multocida. passive hemagglutination (pha) titers to lipopolysaccharide (lps) and the degree of protection conferred by ribosomal vaccines were diminished or abolished when ribosomes were chromatographed on an immunoadsorbent column. addition of subimmunogenic amounts of serotype 1 (homologous) lps to highly purified ribosomes resulted in vaccines that protected against challenge exposure an ... | 1984 | 6208828 |
| [disseminated aspergillosis in chronic respiratory insufficiency. diagnostic and therapeutic aspects]. | | 1983 | 6226984 |
| immune complex crescentic glomerulonephritis associated with pulmonary aspergillosis. | we describe a case of immune complex crescentic glomerulonephritis associated with invasive pulmonary aspergillus fumigatus infection. immunofluorescent and immunoperoxidase investigations showed the presence of aspergillus fumigatus antigen in the glomerular immune complexes. | 1983 | 6229467 |
| effect of castanospermine on the structure and secretion of glycoprotein enzymes in aspergillus fumigatus. | aspergillus fumigatus secretes a number of glycosidases into the culture medium when the cells are grown in a mineral salts medium containing guar flour (a galactomannan) as the carbon source. at least some of these glycosidases have been reported to be glycoproteins having n-linked oligosaccharides. in this study, we examined the effect of the glycoprotein processing inhibitor, castanospermine, on the structures of the n-linked oligosaccharides and on the secretion of various glycosidases. cell ... | 1984 | 6237100 |
| trypsinogen-kinase from aspergillus fumigatus. | the activation of bovine trypsinogen by an extracellular acid proteinase from a. fumigatus is described. the enzyme activates trypsinogen optimally at ph 3.5 and 32 degrees c. the effect of substrate and enzyme concentrations on the activation has been studied and the km-value has been determined. | 1980 | 6247187 |
| the activity of ketoconazole in mixed cultures of fungi and human fibroblasts. | | 1980 | 6247651 |
| opportunistic infections of the lung. | | 1980 | 6252645 |
| [bronchocentric granulomatosis (author's transl)]. | a case report and a review of this recently described entity are presented. a feature of interest in our case is the presence of aspergillus fumigatus at the pulmonary needle biopsy as well as in the surgical specimen. this finding is described for the first time in a non asthmatic patient. | 1981 | 6276988 |
| [ketoconazole in the treatment of pulmonary aspergillosis and aspergilloma: mycological observations (author's transl)]. | | 1982 | 6280948 |
| damage to aspergillus fumigatus and rhizopus oryzae hyphae by oxidative and nonoxidative microbicidal products of human neutrophils in vitro. | our previous studies established that human neutrophils could damage and probably kill hyphae of aspergillus fumigatus and rhizopus oryzae in vitro, primarily by oxygen-dependent mechanisms active at the cell surface. these studies were extended, again quantitating hyphal damage by reduction in uptake of (14)c-labeled uracil or glutamine. neither a. fumigatus nor r. oryzae hyphae were damaged by neutrophils from patients with chronic granulomatous disease, confirming the importance of oxidative ... | 1982 | 6292103 |
| [fungal rhinitis in the dog. diagnosis and therapy]. | | 1982 | 6294907 |
| interactions among amphotericin b, 5-fluorocytosine, ketoconazole, and miconazole against pathogenic fungi in vitro. | interactions among amphotericin b, 5-fluorocytosine, ketoconazole, and micoconazole were tested for all possible paired and triple drug combinations and all four drugs combined against three isolates of candida albicans, three candida spp., two isolates of cryptococcus neoformans, and three isolates aspergillus fumigatus. an assay for inhibitory activity was developed in which growth in the presence of an antifungal agent was expressed as a percentage of the growth in drug-free cultures. for nea ... | 1982 | 6295266 |
| combination therapy of experimental candidiasis, cryptococcosis and aspergillosis in mice. | combination pairs of the major systematic antimycotic drugs, amphotericin b (amphb), 5-fluorocytosine (5-fc) and ketoconazole (ktz) were administered to mice with experimental candidiasis, cryptococcosis and aspergillosis at a variety of combination ratios. the 3 mycoses were produced with 3 strains each of candida albicans, cryptococcus neoformans, and aspergillus fumigatus, respectively, which were preselected to represent 3 different degrees of 5-fc sensitivity ('normally sensitive', 'moderat ... | 1982 | 6297858 |
| mechanisms of destruction of aspergillus fumigatus hyphae mediated by human monocytes. | normal monocytes attached to and destroyed aspergillus hyphae by morphologic and metabolic criteria. inhibition by anaerobiosis, azide, cyanide, halide-free conditions, catalase, histidine, and tryptophan suggested mediation of hyphal damage primarily through the myeloperoxidase system. however, myeloperoxidase-independent oxidative or nonoxidative mechanisms appeared active in hyphal damage by monocytes from patients with myeloperoxidase deficiency or chronic granulomatous disease (cgd). moreov ... | 1983 | 6300255 |
| effects of interferon-alpha on cytomegalovirus reactivation syndromes in renal-transplant recipients. | we have previously demonstrated that six weeks of prophylaxis with interferon-alpha delays cytomegalovirus excretion and decreases viremia in recipients of kidney transplants. in a double-blind trial to evaluate the effects of a longer course of prophylaxis, we gave either 3 x 10(6) units of interferon or placebo intramuscularly to 42 patients before transplant surgery was performed. after surgery, doses were given three times a week for six weeks and then twice a week for eight weeks (total of ... | 1983 | 6304513 |
| treatment of experimental murine aspergillosis with bay n7133. | bay n7133, a recently developed imidazole antifungal drug, was evaluated in experimental murine aspergillosis. balb/c mice were challenged with conidia of aspergillus fumigatus by either the intravenous or the intranasal route. one day after challenge, oral treatment with bay n7133, ketoconazole, or a placebo control was begun bay n7133 was well absorbed after oral administration and penetrated all tissues evaluated. there was some evidence for accelerated inactivation of the drug after prolonge ... | 1983 | 6313822 |
| extrinsic allergic alveolitis in the tobacco industry. | a total of 57 subjects who had been exposed to mould dust in the tobacco industry were studied. their working environment showed exposure to spores of different moulds, and 29 subjects (51%) showed antibodies against one or more of the microbes. fifteen (26%) had work related respiratory symptoms. eight (14%) showed slight radiographic pulmonary fibrosis. spirometry showed a tendency toward restriction and obstruction, especially in small airways. diffusion capacity was decreased in 18% of the w ... | 1984 | 6318802 |
| killing of aspergillus fumigatus spores and candida albicans yeast phase by the iron-hydrogen peroxide-iodide cytotoxic system: comparison with the myeloperoxidase-hydrogen peroxide-halide system. | a new fungicidal system composed of ferrous ion, h2o2, and iodide is described and compared with the myeloperoxidase-hydrogen peroxide-halide system. both systems had similar activity against aspergillus fumigatus spores and the candida albicans yeast phase, but only the ferrous ion-hydrogen peroxide-iodide system was inhibited by hydroxyl radical scavengers. | 1984 | 6321349 |
| studies on experimental aspergillosis in immunodeficient mice. | mice with either chemically suppressed immune response or with specifically induced t cell or b cell immunodeficiency were challenged with aspergillus fumigatus spores. chemical immunosuppressants used were cortisone, cyclophosphamide or silica. t cell deficiency was produced by thymectomy and als administration, whereas, b cell deficiency was produced by administration of anti-mouse igm antibodies in newborn mice. the susceptibility of such animals to a. fumigatus challenge was monitored by mor ... | 1983 | 6326425 |
| in vitro activities of amphotericin b in combination with four antifungal agents and rifampin against aspergillus spp. | strains of aspergillus fumigatus, aspergillus flavus, and aspergillus niger were tested for in vitro susceptibility with a microtiter plate system in buffered yeast-nitrogen base and in buffered minimal essential medium. isolates were tested against amphotericin b, flucytosine, rifampin, ketoconazole, ici 153,066, and bay n 7133 and against combinations of amphotericin b with each of the other five drugs. combinations of amphotericin b and rifampin were the most active against all three species ... | 1984 | 6329088 |
| antimycotic activity of bay n 7133 in animal experiments. | the triazole derivative bay n 7133 has been tested for its antimycotic efficacy on oral administration in vivo and compared with ketoconazole in mice infected with candida albicans, aspergillus fumigatus and cryptococcus neoformans and in guinea pig trichophytosis. on starting administration at the same time as infection and using daily doses between 25 and 100 mg/kg, the agent protected the mice in all experimental models, even mouse aspergillosis for which ketoconazole was not adequately effec ... | 1984 | 6330018 |
| antimycotic azoles. 7. synthesis and antifungal properties of a series of novel triazol-3-ones. | a series of novel triazol-3-ones have been synthesized, and their in vitro and in vivo antifungal properties are reported. compound 68 (itraconazole), which displays a pronounced oral activity against vaginal candidosis in rats and against microsporosis in guinea pigs, has been selected for clinical evaluation. | 1984 | 6330360 |
| immunologic studies in bronchoalveolar fluid in a patient with allergic bronchopulmonary aspergillosis. | bronchoalveolar lavage was performed in a patient during an acute stage of allergic bronchopulmonary aspergillosis (abpa) and repeated 17 mo later during a remission stage and cessation of corticosteroid therapy. measurement of protein concentrations (albumin, igg, iga, and igm) by laser nephelometry and crossed immunoelectrophoresis indicates that transudation of proteins occurs from the circulation into the lung compartment during the acute stage of abpa. furthermore, comparing total ig concen ... | 1984 | 6334111 |
| determination of antibodies to partially purified aspergillus antigens by an enzyme-linked immunosorbent assay. | a total of 75 sera from 17 patients with pulmonary aspergillosis and 58 controls were investigated by elisa for igg antibodies against different antigen fractions of aspergillus fumigatus. somatic antigen obtained by homogenization of young hyphi was fractionated on a sephadex g-200 column, and 11 antigen fractions (i-xi) were prepared. antibodies to fractions i-xi were detected in sera from patients with aspergillosis, whereas antibodies in sera from control patients and healthy blood donors we ... | 1983 | 6337106 |
| use of aspergillus fumigatus mycelial antigens in enzyme-linked immunosorbent assay and counter-immunoelectrophoresis. | antigens obtained from the ruptured mycelium of aspergillus fumigatus were separated on the basis of their attachment or non-attachment to concanavalin a-linked sepharose gel. they were analysed by double-diffusion and two-dimensional immunoelectrophoresis with rabbit antiserum and sera from patients with aspergillus-related disease. their sensitivity was assessed in an enzyme-linked immunosorbent assay (elisa) for immunoglobulin g. sera which gave very low or high extinction readings on elisa g ... | 1983 | 6337264 |
| invasive pulmonary aspergillosis associated with aspergilloma in sarcoidosis. | we have described two patients with corticosteroid-treated sarcoidosis and with autopsy-proven aspergilloma and diffuse invasive pulmonary aspergillosis. in one, growth of the fungus within a cyst was probably followed by progression to diffuse pulmonary infection. in the second, lung necrosis from invasive infection may have led to the formation of the mycetoma found at autopsy. | 1983 | 6337409 |
| [the litter - aspergillus - mastitis]. | a number of cases of bovine mastitis caused by aspergillus fumigatus are reported. the animals showed considerably swollen hard quarters associated with the production of large quantities of watery secretion and symptoms of systemic disease. both intramammary and systemic treatment with several antibacterial agents failed to produce any improvement, nor did an antimycotic administered by intramammary route result in improvement. severe mastitis marked by mycotic lesions was observed on slaughter ... | 1983 | 6338618 |
| immunological significance of aspergillus fumigatus in cane-sugar mills. | an aeromycological study has revealed the preponderance of aspergillus fumigatus at the bagasse-containing sites in sugar-cane mills located in the northwestern part of the punjab state. the fungus was frequently isolated from the sputum cultures of the mill workers and a significant number of the workers were positive reactors when challenged intracutaneously with aspergillin. immediate type i and late arthus type skin hypersensitivity was observed in 10.6% and 4.4%, respectively, of the 548 wo ... | 1983 | 6338838 |
| aspergillus fumigatus endocarditis on a normal heart valve. | we have described an elderly man with no known underlying predisposing systemic or valvular disease who had mitral valve infection and endophthalmitis due to aspergillus fumigatus. two-dimensional echocardiography was valuable in detecting fungal vegetations. | 1983 | 6340215 |
| orbital aspergillosis. report of a case diagnosed by fine needle aspiration biopsy. | presentation is made of a case of orbital aspergillosis, a rare condition that may clinically mimic nonspecific orbital inflammatory disease. it can be a subacute or chronic disease that develops in otherwise healthy individuals. fine needle aspiration biopsy, which has been useful in the diagnosis of a variety of orbital diseases, provided the initial diagnosis in this case. | 1983 | 6340402 |
| fungal protein produced on cassava for growing rats and pigs. | | 1983 | 6341343 |
| enzyme-linked immunosorbent assay and its application to the serological diagnosis of fungal infection. | | 1983 | 6342173 |
| natural atmospheric microbial conditions in a typical suburban area. | ambient outdoor concentrations and size distributions of airborne microbial particles were measured approximately weekly for 2 years in a washington, d.c., suburban area. the study objective was to characterize microbial air quality in the vicinity of a proposed sewage sludge composting facility. during the study, 379 samples were taken at 17 stations, using andersen microbial samplers. concentration ranges (in viable particles per cubic meter) were as follows: airborne mesophilic fungi, 0 to 7, ... | 1983 | 6342536 |
| antifungal activity of enilconazole on experimental aspergillosis in chickens. | day-old chickens were infected with various inoculum sizes of airborne spores of aspergillus fumigatus, which caused high rates of mortality and morbidity. fumigation with enilconazole smoke pellets was highly successful in preventing mortality, in reducing morbidity, and in neutralizing growth inhibition of infected chickens. no side effects were observed. | 1983 | 6342595 |
| mould counts and exacerbations of allergic bronchopulmonary aspergillosis. | the purpose of this study was to determine whether exacerbations of allergic bronchopulmonary aspergillosis (abpa) were associated with the total outdoor mould counts in the chicago area. from 1976-1980, forty-nine flares of abpa (new pulmonary infiltrate with elevation in total serum ige) occurred in nineteen patients. thirty-eight (77.5%) of flares occurred during months june through november in association with increased outdoor mould counts in chicago. this association confirms earlier obser ... | 1983 | 6342846 |
| a non-immunosuppressed patient with presumably invasive pulmonary aspergillosis and a favourable outcome. | | 1983 | 6343901 |
| orbital aspergillosis. | we treated a 40-year-old male who had orbital aspergillosis and presumed early intracranial extension with limited orbital exenteration and adjunctive amphotericin b. forty-two months later the patient is alive without recurrence. our patient illustrates the typical presentation of orbital aspergillosis with severe periorbital pain and proptosis; however, he did not have the usual concomitant sinusitis. aggressive surgical and antifungal therapy in this case was successful in preventing further ... | 1983 | 6343937 |
| [immunofluorescence method for the detection of fungi in bronchial secretions]. | a method for detecting fungal elements in bronchial secretions with the aid of indirect immunofluorescence is described. advantages of this method are the white fluorescence of fungal elements on dark background, whereas conventional staining methods (grocott, pas) also stain mucus. further on it is possible to demonstrate with this immunofluorescence test the presence of antibodies against fungi in patients sera. advantages and disadvantages of this method are discussed. | 1983 | 6344455 |
| an atypical isolate of aspergillus fumigatus from a man affected with a pulmonary disease. | | 1983 | 6346091 |
| a study on the possible role of chymotrypsin in the aetiology of equine chronic obstructive pulmonary disease (copd). | the chymotrypsin activity of seven batches of micropolyspora faeni and of five batches of aspergillus fumigatus culture extracts, prepared for inhalation challenge in horses, was assayed and was found to range between 0.29 and 1.45 units/mg protein and 0.02 and 0.20 units/mg protein respectively. horses affected with chronic obstructive pulmonary disease (copd) were challenged with two batches of each antigen which had different chymotrypsin activities and no significant correlations were found ... | 1983 | 6346665 |
| complement-binding antibodies to aspergillus fumigatus in patients with pulmonary aspergillosis. | complement-binding antibodies against aspergillus fumigatus were determined by a fluorescent antibody technique with purified human clq as the source of complement. clq binding aspergillus antibodies were found in 8 (67%) of 12 patients with pulmonary aspergillosis, in 4 (57%) of 7 patients with suspected pulmonary aspergillosis, in one (14%) of 7 patients with precipitating antibodies to a. fumigatus of undetermined clinical significance, and in 4 (7%) of 54 healthy blood donors. sera from pati ... | 1983 | 6346788 |
| levels of gram-negative bacteria, aspergillus fumigatus, dust, and endotoxin at compost plants. | airborne gram-negative bacteria, endotoxins, dust, and aspergillus fumigatus were measured in four compost plants in sweden. at sites where material was processed, the number of airborne a. fumigatus exceeded 10(6)/m3, whereas the number of gram-negative bacteria was usually lower. dust levels were moderate, and endotoxin levels were well below 0.5 micrograms/m3. medical studies to evaluate the effects of this type of microbial exposure are recommended. | 1983 | 6347061 |
| antigens of aspergillus fumigatus. ii. electrophoretic and clinical studies. | cell sap (cs) and culture filtrate (cf) preparations of aspergillus fumigatus strains ag-507, ag-515, and ag-534 were analysed by two dimensional electrophoresis (2-de; i.e., first dimension isoelectric focusing, second dimension sodium dodecyl sulphate gradient pore gel), which enabled detection of strain- and species-specific components. in cs preparations it was shown that cs2, a fraction isolated from strain ag-507 by gel filtration, consists of the major protein components in the cs of the ... | 1983 | 6347877 |
| immunochemical characterization of aspergillus fumigatus antigens. | culture filtrate antigens of aspergillus fumigatus ag 534 were purified by preparative isoelectric focusing and affinity chromatography. one of the pooled antigen fractions from the preparative isoelectric focusing step (pool 2) was passed through a concanavalin a column and yielded two components, designated antigens iia and iib. antigen iib reacted more strongly than antigen iia with all of the aspergilloma and allergic bronchopulmonary aspergillosis sera tested by enzyme-linked immunosorbent ... | 1983 | 6347893 |
| [aspergillus mycoses of the paranasal sinuses. detection and analysis of roentgen opaque structures in fungal concretions]. | out of 59 patients with aspergillosis of the paranasal sinuses 25 showed almost metal-dense x-ray-shadows in highmore's sinus, resembling foreign bodies at first. it can be demonstrated, that these areas are equivalent to local enrichment of calcium phosphate mainly in the center of the fungal masses in the sinuses. technique and methods of investigation and analysis are described. x-ray findings like these should be looked upon as a sign reading: "aspergillosis of the paranasal sinuses ahead." | 1983 | 6347993 |
| humoral and cellular immune responses of atopic individuals in a tropical environment. | in this study we compared immediate, intermediate and delayed skin test reactivity, total and specific serum ige, igg, a, m antibody and lymphocyte proliferative responses, between atopic and normal individuals in the tropical environment of caracas, venezuela (lat. 10 degrees n). the allergenic extracts tested were prepared from house dust, mixed moulds and aspergillus fumigatus. in lymphocyte stimulation the mitogen concanavalin a was also employed, the cultures being supplemented with either ... | 1983 | 6348297 |
| allergenic fungi and actinomycetes in smoking materials and their health implications. | street marijuana, commercial cigarettes and pipe tobaccos were studied for the presence of fungi and actinomycetes associated with hypersensitivity pneumonitis. aspergillus species and thermophilic actinomycetes were isolated from the smoking materials. in addition, aspergillus fumigatus spores were isolated from marijuana smoke, indicating the potential hazard involved in developing serious disease. precipitin antibodies against fungi, particularly aspergillus, showed a higher prevalence in mar ... | 1983 | 6348548 |
| [allergic bronchopulmonary aspergillosis. apropos of 30 cases]. | thirty cases of allergic bronchopulmonary aspergillosis (abpa) were treated between 1967 and 1981. developing in patients with a history of chronic asthma (28 of the 30 cases), the initial manifestations of abpa developed after long periods (an average of 29 years after the onset of the asthma). chest radiography demonstrated recurrent labile infiltrates in 28 cases, segmental or lobar atelectasis in 7, and proximal bronchiectasis in 16 cases. a circulating eosinophilia was a constant finding, b ... | 1983 | 6348723 |
| serum antibodies to aspergillus fumigatus in patients with rheumatic diseases. | igg and iga serum antibodies to aspergillus fumigatus were determined in 47 patients with rheumatic disorders, 4 patients with pulmonary aspergillosis associated with rheumatic disease, and in 36 healthy controls. antibody titres determined by indirect immunofluorescence were comparable in patients with rheumatic diseases and in controls, except for 2 patients with igg antibody titres within the upper range of patients with aspergillosis. igg antibody levels to 2 partially purified a. fumigatus ... | 1983 | 6348977 |
| isolation of cryptococcus neoformans, candida albicans and other yeasts from pigeon droppings in egypt. | two hundred pigeon droppings, unmixed with soil, and collected from various provinces in lower egypt (delta) were cultured to detect yeasts. yeasts were isolated from 53.5% of the samples; cryptococcus neoformans was recovered from 30 samples and candida albicans from 19 samples. other cryptococcus and candida species as well as species of torulopsis and rhodotorula were also encountered. aspergillus fumigatus was isolated on 13 occasions. | 1983 | 6348978 |
| ankylosing spondylitis lung disease--an underdiagnosed entity? | during the last 10 years, 14 cases of ankylosing spondylitis (as) with upper lobe changes were diagnosed. this is certainly an underestimation, since as is often not noted in the case records even when diagnosed. two recent cases were earlier undiagnosed, and only on specific questioning did the patients admit symptoms, though they were both hla b27 positive and had x-ray changes of the sacro-iliacal joints typical of the disease. thus, if not carefully sought, the diagnosis can be missed. a lit ... | 1983 | 6350034 |
| standardization of antigens from aspergillus fumigatus. | batches of soluble antigen prepared by mechanical disintegration of aspergillus fumigatus reproducibly contained identical protein components on polyacrylamide gels. this consistency was ascribed to standardization at the procedure for disruption: there was little variation in the protein patterns obtained from a. fumigatus mycelium grown for times varying from one to seven days at temperatures from 27 to 37 degrees c. rabbit antisera to different a. fumigatus antigen preparations varied in thei ... | 1983 | 6350304 |
| invasive aspergillosis in renal transplant recipients: correlation with corticosteroid therapy. | during a 31-month period in 1979-1981, nine patients at a renal transplant center in tennessee developed invasive infections with aspergillus species. despite an extensive search, no common environmental source of contamination was found. a matched case-control study of host risk factors showed that leukopenia, prior administration of antibiotics, and treatment with azathioprine and antilymphocyte serum were not significantly related to the development of aspergillosis. in contrast, the administ ... | 1983 | 6350492 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 38-1983. empyema 40 years after a thoracoplasty. | | 1983 | 6350875 |
| [pulmonary aspergillosis with abscess formation]. | | 1983 | 6351394 |
| keratomycosis in a buffalo calf (bubalus bubalis) caused by aspergillus fumigatus. | | 1983 | 6351411 |
| mycotic invasion of the mare's uterus. | uterine infections in three mares following repeated uterine manipulations and antibiotic therapy were caused by aspergillus fumigatus in two cases and candida albicans in the third. two of the infections were noticed during dioestrus and one at oestrus and one mare produced a purulent vulval discharge. large numbers of neutrophils, increased protein concentrations and the presence of fungal hyphae in flushings from the uterine lumen were indicative of active infection. the mycotic infections ca ... | 1983 | 6351412 |
| circulating immune complexes and rheumatoid factors in patients with farmer's lung. | circulating igg immune complexes (cics), igm rheumatoid factors (rfs), complement level (c3 and c4), and igg antibodies to aspergillus fumigatus, aspergillus umbrosus, thermoactinomyces vulgaris, and micropolyspora faeni were analysed in the sera of 14 patients with farmer's lung (fl), 10 in the acute and four in the subacute phase of the disease. ten spouses of fl patients served as exposed healthy controls. c3 and c4 were measured fluoronephelometrically. c3 levels were above the normal range ... | 1983 | 6351661 |
| purification of the major endoglucanase from aspergillus fumigatus fresenius. | aspergillus fumigatus (fresenius), imi 246651, a.t.c.c. 46324, produces two beta-glucosidase enzymes, cotton-solubilizing activity, xylanase and endoglucanase enzymes which can be separated by gel-filtration chromatography. the major endoglucanase does not bind to concanavalin a-sepharose and does not stain with periodic acid/schiff reagent. it is homogeneous on polyacrylamide isoelectric focusing (pi = 7.1) and has a mol.wt. of 12500 by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis ... | 1983 | 6351841 |
| [3 cases of naso-sinal aspergillosis]. | | 1983 | 6352216 |
| pathogenicity of aspergillus fumigatus in chicks, guinea fowl, rabbits and mice. | | 1983 | 6353224 |
| role of endotoxin of aspergillus fumigatus in its pathogenicity. | | 1983 | 6353225 |
| intracerebral aspergillus abscess: case report. | intracranial aspergillosis is rare and often fatal. the authors report the successful treatment of a right temporal intracerebral aspergillus abscess. | 1983 | 6353266 |
| localized invasive pulmonary aspergillosis treated by surgical excision. | invasive aspergillosis developed in a patient without preexisting lung disease or immunosuppression, after she had received only a one-month course of corticosteroid therapy. surgical excision, without antifungal therapy, resulted in eradication of the infection. | 1983 | 6353603 |
| aspergillus fumigatus and other thermotolerant fungi generated by hospital building demolition. | on 13 september 1981, a 51-year-old seven-story building within our hospital complex was demolished by explosives. the concern that this event might release large numbers of thermotolerant fungi (tf), potentially hazardous to immunosuppressed patients, led us to seal hospital windows and doors. the air-handling systems were also manipulated. concentrations of airborne tf, especially aspergillus fumigatus, were determined before and after demolition, using andersen and cassella air samplers with ... | 1983 | 6354086 |
| [combined treatment of corneal aspergillosis using the argon laser and clotrimazole]. | | 1983 | 6355634 |
| recovery of aspergillus fumigatus aerospora from municipal sewage sludge composting operations in the state of maine. | an investigation was conducted to determine the effect of composting activity on ambient and transient viable aspergillus fumigatus aerospora at three composting operations (westbrook, bangor, old town) in the state of maine. meteorological conditions and seasonality were also considered. the purpose of the study was to determine whether composting operations caused increased public health risks due to the generation and dispersal of elevated a. fumigatus aerospora concentrations. ambient aerosp ... | 1983 | 6355853 |
| [pleural aspergillosis]. | | 1983 | 6356126 |
| comparison of partially purified mycelial and culture filtrate antigens of aspergillus fumigatus by enzyme-linked immunosorbent assay. | an aqueous extract of aspergillus fumigatus mycelium was fractionated by precipitation with ammonium sulphate to give protein-enriched and carbohydrate-enriched preparations. the antigenic activity of the fractions so obtained was assessed in an enzyme-linked immunosorbent assay using pooled positive and negative reference sera. the best discrimination between such sera was obtained when using either the fraction insoluble in 50--75% ammonium sulphate or the material soluble in a saturate soluti ... | 1983 | 6356408 |
| serologic testing of wild roe deer (capreolus capreolus l.) from the trois fontaines forest region of eastern france. | | 1983 | 6358537 |
| [pilot study on the incidence of aspergillus fumigatus in in mucoviscidosis patients]. | | 1983 | 6359121 |
| antigen detection in the diagnosis of invasive aspergillosis. utility in controlled, blinded trials. | two blinded, controlled trials were done to evaluate the usefulness of fungal antigen detection for the diagnosis of invasive aspergillosis. detection of aspergillus fumigatus carbohydrate by radioimmunoassay was compared with antibody detection by an enzyme-linked immunosorbent assay and with diagnostic microbiologic and histopathologic procedures. in the first trial, antigenemia was detected in 4 of 6 leukemic patients with invasive pulmonary aspergillosis, but not in 8 acute leukemic controls ... | 1983 | 6359996 |
| correlation of elastase production by some strains of aspergillus fumigatus with ability to cause pulmonary invasive aspergillosis in mice. | seventy-five strains of aspergillus fumigatus were screened for production of elastase in liquid and agar media containing elastin in yeast carbon base buffered with 0.05 m borate, ph 7.6. of 71 strains which cleared elastin in agar plates, 33 produced elastase in liquid medium, as measured spectrophotometrically with elastin-congo red. six strains producing elastase and four nonproducers were tested for ability to cause invasive aspergillosis in immunocompromised mice (six mice per strain). all ... | 1984 | 6360904 |
| [photocoagulation of mycotic fundus changes in rabbits]. | | 1983 | 6361363 |
| [study of fungal flora of the skin of patients with psoriasis]. | | 1983 | 6361912 |
| influence of econazole and 5-fluorocytosine on the ultrastructure of aspergillus fumigatus and the cytochemical localization of calcium ions as measured by laser microprobe mass analysis. | the ultrastructural changes and the localization of ca++ ions have been investigated in aspergillus fumigatus after exposure in vitro to econazole (50 micrograms ml-1) and to 5-fluorocytosine (100 micrograms ml-1) for 24 h. the changes obtained with econazole concerned the cell periphery, necrotization of mitochondria and hyperactivity of the central vacuole. after cytochemical ca++ localization a marked increase in precipitate was observed on mitochondria, vacuoles and collapsed membranes, comp ... | 1983 | 6362038 |
| antigenic variability of different strains of aspergillus fumigatus. | 10 strains (isolates) of aspergillus fumigatus, grown and harvested under identical conditions, were compared with respect to biochemical and immunological properties. the total protein and carbohydrate content isoelectric focusing pattern were determined for each of the individual strains. their antigenic and allergenic activity was examined by a combination of crossed immunoelectrophoresis (cie)/crossed radioimmunoelectrophoresis (crie) and rast inhibition. biochemical and immunochemical data ... | 1984 | 6363306 |
| antigen and antibody attachment in elisa for aspergillus fumigatus igg antibodies. | four commercial antigen extracts of aspergillus fumigatus were evaluated for use in a rapid enzyme-linked immunosorbent assay (elisa) for anti-a. fumigatus igg. initial binding of both somatic and culture filtrate preparations to a polyvinyl chloride solid phase was concentration dependent and increased with incubation time. antigen binding to the solid phase was reproducible. binding of a. fumigatus precipitin-positive serum to bound antigen was rapid. all four a. fumigatus antigens demonstrate ... | 1984 | 6363547 |
| [detection of anti-aspergillus igg type antibody in the sera by solid phase immunoradiometric assay--comparison with gel precipitation test in cases of pulmonary aspergilloma]. | | 1983 | 6363780 |
| mycotic abortions in equines. | | 1983 | 6363920 |
| hypersensitivity pneumonitis due to aspergillus fumigatus in compost. | | 1984 | 6364446 |
| experimental murine aspergillosis. comparison of amphotericin b and a new polyene antifungal drug, sch 28191. | in a model of experimental murine aspergillosis, efficacy of amphotericin b (amb) desoxycholate was compared with sch 28191, the d-ornithyl methyl ester derivative of amphotericin b (sch). in vitro studies showed that the aspergillus isolate was equally susceptible to amb and sch. both drugs were equally effective in prolonging survival after intravenous challenge with conidia of a. fumigatus. this was apparent when treatment was begun 24 h after challenge or delayed until mice were moribund; sc ... | 1984 | 6364905 |
| evaluation of a rapid enzyme-linked immunosorbent assay for igg antibodies to aspergillus fumigatus in the serological diagnosis of allergic aspergillosis. | a range of 6 somatic and culture filtrate antigens of aspergillus fumigatus were evaluated in a rapid elisa procedure for anti-a. fumigatus igg where the component incubation times had been reduced to 10 min. sera from patients with allergic aspergillosis, patients with suspected allergic aspergillosis, and asthmatic patients with or without a. fumigatus precipitins were tested. for all antigens, levels of anti-a. fumigatus igg were higher in patients with allergic aspergillosis than in the othe ... | 1984 | 6365791 |
| varying presentations of allergic bronchopulmonary aspergillosis. | the diagnosis of allergic bronchopulmonary aspergillosis (abpa) may be difficult when the major criteria are not present or when the clinical, laboratory, and radiographic findings have been altered by prior corticosteroid therapy. however, the early diagnosis of abpa is important because it may lead to the prevention of evolution to the corticosteroid-dependent asthma stage (stage iv) or fibrotic lung disease stage (stage v). the clinical histories of 3 abpa patients are reviewed to demonstrate ... | 1984 | 6365793 |
| defective phagocyte aspergillus killing associated with recurrent pulmonary aspergillus infections. | an apparently healthy boy was suffering from recurrent aspergillus infections. no classical conditions of immunodeficiency were found. studies on the patient's phagocytic system revealed neutrophils and monocytes to function normally except in aspergillus killing (microbicidal activity for bacteria and candida was normal). aspergillus killing mechanisms may be complex and peculiarly selective, possibly involving both oxygen-dependent and independent mechanisms. | 1984 | 6368399 |
| pulmonary infiltrates in immunocompromised patients: diagnosis by cytological examination of bronchoalveolar lavage fluid. | thirty pulmonary infiltrates in 26 patients were investigated by bronchoalveolar lavage. sixteen of the patients were on therapeutic immunosuppression for renal disease or transplant and 10 had leukaemia, lymphoma, or allied conditions. a rapid specific diagnosis was made in 21 (70%) episodes by cytological examination of the fluid and in 28 (93%) by a combination of cytology and microbiology. no complications from haemorrhage or pneumothorax ensued. pneumonia due to pneumocystis carinii was the ... | 1984 | 6368604 |
| aspergillus terreus endocarditis--a case report. | | 1983 | 6368727 |
| pathogenesis of experimentally produced corneal ulcers in rabbits. | intracorneal injection of staphylococcus aureus and aspergillus fumigatus, both separately and together, produced corneal ulcer in rabbits without pretreatment of the eyes with steroids or antibiotics. staphylococcus alone produced self-limiting ulcers. aspergillus alone produced severe deep ulcers ending in perforations. bacterial infection followed by introduction of fungus produced less severe ulcers than did fungus alone. established fungal infection followed by super-imposed bacterial infec ... | 1984 | 6370074 |
| interaction of aspergillus fumigatus spores and pulmonary alveolar macrophages of rabbits. | the in vitro and in vivo interaction of rabbit pulmonary alveolar macrophages (pam) and aspergillus fumigatus spores was studied. in vitro experiments showed that pam from normal rabbits failed to appreciably kill a. fumigatus spores in 4 hours, while a. flavus and a. niger spores were destroyed effectively. prior opsonization of the spores with normal rabbit serum, rabbit anti-a. fumigatus serum, complement or lung lavage fluid has no profound enhancing effect on the phagocytosis or killing of ... | 1984 | 6370839 |
| severe disseminated aspergillosis in a captive abyssinian tawny eagle (aquila rapax raptor). | | 1984 | 6371262 |
| [disseminated aspergillosis in chronic respiratory insufficiency. diagnosis and treatment]. | | 1984 | 6371398 |
| [microbiological and clinical analysis disease cases caused by occupational contact with beech scraps in a cellulose plant]. | | 1984 | 6371721 |
| spinal cord compression due to an aspergilloma. | aspergilloma is a common complication of cystic lung disease; it is widely considered to be a saprophytic colonization and local or disseminated invasion is unusual. we describe a patient with stage iv sarcoidosis and bilateral aspergillomas in whom locally invasive disease led to spinal cord compression. | 1984 | 6372110 |
| antigen selection for optimal serological diagnosis of aspergillus fumigatus infection of the nasal chambers of the dog. | | 1984 | 6372229 |
| aspergillosis in turkeys associated with use of contaminated litter. | severe mortality occurred in a flock of 16,000 turkey poults after fresh litter consisting of poplar, oak, sweetgum, and pine shavings was added to the brooder house. signs and lesions seen were consistent with aspergillosis. mortality for the first 21 days of brooding was 305 poults. new litter was added on day 21; 6,041 poults died during the next 24 days. mold counts were done on the original litter and added litter. the mold counts for the original litter was 1.0 x 10(5) organisms per gram o ... | 1984 | 6372777 |