| prevalence and distribution of neospora caninum in water buffalo (bubalus bubalis) and cattle in the northern territory of australia. | the seroprevalence of neospora caninum infection in water buffalo (bubalus bubalis) and domestic cattle in the northern territory (nt) of australia has never been determined. a total of 480 serum samples from water buffalo and 192 serum samples from cattle, collected by the nt government from 1993 through to 2001, at 18 different survey sites throughout the northern territory were tested by commercial elisa for anti-n. caninum antibodies. the water buffalo samples demonstrated a seroprevalence o ... | 2015 | 25992666 |
| seroprevalence and risk factors associated with exposure of water buffalo (bubalus bubalis) to neospora caninum in northeast thailand. | water buffalo are important draft animals for agriculture in resource-restricted areas worldwide. water buffalo were shown to be experimentally susceptible to infection with neospora caninum, potentially affected by neosporosis, and naturally exposed to the parasite in asia. although enzootic to thailand, the distribution of n. caninum among thai water buffalo is unclear. the objectives of this study were to determine the seroprevalence of n. caninum among water buffalo of northeast thailand and ... | 2015 | 25499824 |
| abortion and foetal lesions induced by neospora caninum in experimentally infected water buffalos (bubalus bubalis). | the water buffalo (bubalus bubalis) is an important species in several countries for its milk and meat production, as well as for transport and other agricultural activities. it is, in general, considered more resistant than cattle to different parasitic diseases, also less demanding for forage quality. it has been postulated that buffalo may be resistant to abortion caused by neosporosis, because of high serological prevalences found in buffalo herds from different localities, with no descripti ... | 2015 | 25324135 |
| neospora caninum serostatus is affected by age and species variables in cohabiting water buffaloes and beef cattle. | the aim of this study was to investigate how neospora caninum serostatus may be affected by variables such as host species (water buffaloes or cattle) and age in animals cohabiting in the same ranch. a convenience cross-sectional study was performed on four ranches in the northeast of argentina, where water buffalo are cohabitating with beef cattle. blood samples were collected from 1350 female water buffaloes (bubalus bubalis) and 880 female beef cattle (bos taurus and bos indicus crossbreeds) ... | 2014 | 24792747 |
| pcr detection of neospora caninum in water buffalo foetal tissues. | the seroprevalence of neospora caninum was surveyed by an elisa kit on two water buffalo herds of southern italy. seropositive samples were detected in 47% and 59% of individuals, respectively, thus indicating high level of exposure to the parasite even if the possibility of vertical transmission cannot be excluded. tissue samples collected from three aborted fetuses from the same herds were investigated for n. caninum presence by pcr assays targeting the 18s and the nc5 dna sequences, respectiv ... | 2014 | 24570043 |
| characterization of immune cell infiltration in the placentome of water buffaloes (bubalus bubalis) infected with neospora caninum during pregnancy. | neospora caninum infection in cattle stimulates host immune responses, which may be responsible for placental damage leading to abortion. susceptibility of water buffaloes (bubalus bubalis) to neosporosis is not well understood, although vertical transmission and fetal death have been documented. the aim of this study was to characterize the immune response in the placentome of water buffalo following experimental infection in early gestation with the nc-1 strain of n. caninum. placentomes were ... | 2014 | 24529512 |
| genetic characterization of ovine herpesvirus 2 strains involved in water buffaloes malignant catarrhal fever outbreaks in southern italy. | ovine herpesvirus 2 (ovhv-2) was responsible for two outbreaks of malignant catarrhal fever (mcf) on two water buffalo farms in southern italy. in this study, the presence of this virus in the nasal swabs from sick animals as well as in the organs of dead buffaloes was ascertained by a real-time pcr assay. positive samples also underwent a relative quantitative analysis of the viral dna in them. all the dead animals had the highest relative viral quantities, while buffaloes recovering from the v ... | 2017 | 28110782 |
| malignant catarrhal fever: an emerging disease in the african buffalo (syncerus caffer). | within the tribe bovini in the subfamily bovinae, the water buffalo (bubalus bubalis), american bison (bison bison), european bison (bubalus bonasus) and yak (bos grunniens) are recognized as species highly susceptible to malignant catarrhal fever (mcf). in contrast, the lack of reports describing clinical mcf in the african buffalo (syncerus caffer) whether free ranging or captive has led to a perception that african buffaloes are resistant to mcf. during the last decade, several cases of mcf i ... | 2015 | 23957274 |
| involvement of the nervous system following experimental infection with pasteurella multocida b:2 in buffalo (bubalus bubalis): a clinicopathological study. | haemorrhagic septicaemia (hs) is an acute, fatal, septicaemic disease of cattle and buffaloes caused by one of two specific serotypes of pasteurella multocida b:2 and e:2 in asian and african, respectively. it is well known that hs affect mainly the respiratory and digestive tracts. however, involvement of the nervous system in pathogenesis of hs has been reported in previous studies without details. in this study, nine buffalo calves of 8 months old were distributed into three groups. animals o ... | 2016 | 26850845 |
| draft genome sequence of pasteurella multocida subsp. multocida b:2 strain vtccbaa264 isolated from bubalus bubalis in north india. | the pasteurella multocida subsp. multocida b:2 serotype causes hemorrhagic septicemia in bubalines with high morbidity and mortality in the indian subcontinent. we report the draft genome sequence of pasteurella multocida strain vtccbaa264 isolated from the small-intestine of a buffalo calf that died of high fever. | 2014 | 25081265 |
| pathology of experimental infection by pasteurella multocida serotype a: 1 in buffalo calves. | pasteurella multocida serotype a:3 has been mostly implicated in pneumonic pasteurellosis in ruminants. in contrast, our previous studies have reported that both serotypes a:1 and a:3 were responsible for respiratory diseases in cattle and buffaloes. however, the pathology and pathogenesis of p. multocida serotype a:1 (pm a:1) infection have not been studied in ruminants. in the present study, 12- to 15-week-old buffalo calves (bubalus bubalis) infected by pm a:1 had fibrinous and suppurative br ... | 2014 | 24407559 |
| correction: toll-like receptor responses to peste des petits ruminants virus in goats and water buffalo. | | 2015 | 25634030 |
| toll-like receptor responses to peste des petits ruminants virus in goats and water buffalo. | ovine rinderpest or goat plague is an economically important and contagious viral disease of sheep and goats, caused by the peste des petits ruminants virus (pprv). differences in susceptibility to goat plague among different breeds and water buffalo exist. the host innate immune system discriminates between pathogen associated molecular patterns and self antigens through surveillance receptors known as toll like receptors (tlr). we investigated the role of tlr and cytokines in differential susc ... | 2014 | 25369126 |
| characterisation of a babesia orientalis apical membrane antigen, and comparison of its orthologues among selected apicomplexans. | in the present study, we identified and characterised the complete coding sequence of babesia orientalis apical membrane antigen 1 (designated bo-ama1); it is 1803bp in length and encodes a polypeptide of 601 amino acids (aa). the bo-ama-1 gene product (bo-ama1) is predicted to be 67kda in size and contains a signal peptide. mature bo-ama1 is predicted to have one transmembrane region and a short cytoplasmic tail (c-terminal domain). the extracellular part of bo-ama1 has three functional domains ... | 2015 | 25732411 |
| mitochondrial genome of babesia orientalis, apicomplexan parasite of water buffalo (bubalus babalis, linnaeus, 1758) endemic in china. | apicomplexan parasites of the genus babesia, theileria and plasmodium are very closely related organisms. interestingly, their mitochondrial (mt) genomes are highly divergent. among babesia, babesia orientalis is a new species recently identified and specifically epidemic to the southern part of china, causing severe disease to water buffalo. however, no information on the mt genome of b. orientalis was available. | 2014 | 24580772 |
| identification and genetic characterization of rabies virus from egyptian water buffaloes (bubalus bubalis) bitten by a fox. | rabies is caused by negative strand rna-virus classified in the genus lyssavirus, family rhabdoviridae of the order mononegavirales. the aim of the present study was to identify and analyze nucleotides sequence of nucleoprotein (n) gene of rabies virus (rabv) from two cases of water buffaloes (bubalus bubalis) bitten by a fox in egypt, 2013. the diseased buffaloes showed nervous manifestations with fever. specimens from brains of the buffaloes with suspected rabies were collected. rabv in collec ... | 2015 | 26396980 |
| comparative evaluation of rumen metagenome community using qpcr and mg-rast. | microbial profiling of metagenome communities have been studied extensively using mg-rast and other related metagenome annotation databases. although, database based taxonomic profiling provides snapshots of the metagenome architecture, their reliability needs to be validated through more accurate methods. here, we performed qpcr based absolute quantitation of selected rumen microbes in the liquid and solid fraction of the rumen fluid of river buffalo adapted to varying proportion of concentrate ... | 2013 | 24025701 |
| changes in the physico-chemical and microbial quality during the production of pastırma cured with different levels of sodium nitrite. | pastırma, a dry-cured meat product, is produced from the whole muscle and/or muscles obtained from certain parts of beef and water buffalo carcasses. the purpose of this study was to determine the effects of different levels of sodium nitrite on changes in the physicochemical and microbial quality during the production of pastırma. the changes in residual nitrite, salt, ph, moisture, thiobarbutiric acid reactive substances (tbars), colour (l*, a*, b*), total aerobic mesophilic bacteria (tamb), l ... | 2016 | 27857537 |
| metabolomics of the antipyretic effects of bubali cornu (water buffalo horn) in rats. | bubali cornu (water buffalo horn, wbh) has been used for thousands of years in traditional chinese medicine (tcm) as an effective treatment for heat. in the present study, we have carried out a metabolomics profiling study on plasma and urine samples to explore potential biomarkers and determine how wbh exerts its antipyretic effects in yeast-induced pyrexia at a metabolomic level. ultra-high performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (uplc- ... | 2016 | 27384078 |
| complete genome sequence of a lytic siphoviridae bacteriophage infecting several serovars of salmonella enterica. | the bacteriophage 100268_sal2 was isolated from water buffalo feces in southern italy, exhibiting lytic activity against several subspecies of salmonella enterica this bacteriophage belongs to the siphoviridae family and has a 125,114-bp double-stranded dna (ds-dna) genome containing 188 coding sequences (cdss). | 2016 | 27688334 |
| complete genome sequences of three siphoviridae bacteriophages infecting salmonella enterica serovar enteritidis. | three bacteriophages, 118970_sal1, 118970_sal2, and 64795_sal3, were isolated from water buffalo feces in southern italy, exhibiting lytic activity against salmonella enterica serovar enteritidis. these bacteriophages belong to the siphoviridae family and have a 60,113-bp, 123,930-bp, and 48,094-bp double-stranded dna (dsdna) genome containing 72, 173, and 80 coding sequences (cdss), respectively. | 2016 | 27856572 |
| molecular differentiation of sarcocystis buffalonis and sarcocystis levinei in water buffaloes (bubalus bubalis) from sarcocystis hirsuta and sarcocystis cruzi in cattle (bos taurus). | the purpose of the present study was to obtain sarcocysts of sarcocystis buffalonis and sarcocystis levinei from water buffaloes and characterize the isolates by molecular methods in order to determine whether the two species were genetically different from sarcocystis hirsuta and sarcocystis cruzi, respectively, from cattle, which had been characterized before. about 35 macroscopically visible (3-4 × 1-2 mm) and 20 barely visible (1-3 × 0.2 mm) sarcocysts were excised from the esophagus of 18 n ... | 2016 | 26979729 |
| molecular characterisation of sarcocystis bovifelis, sarcocystis bovini n. sp., sarcocystis hirsuta and sarcocystis cruzi from cattle (bos taurus) and sarcocystis sinensis from water buffaloes (bubalus bubalis). | about 200 individual sarcocysts were excised from 12 samples of cattle beef from five countries (argentina, brazil, germany, new zealand, uruguay) and tentatively identified to species or cyst type on the basis of their size and shape and cyst wall morphology. genomic dna was extracted from 147 of these sarcocysts and used initially for pcr amplification and sequencing of the partial mitochondrial cytochrome c oxidase subunit i gene (cox1) in order to identify the sarcocysts to species and/or se ... | 2016 | 26677095 |
| microscopical and serological studies on sarcocystis infection with first report of s. cruzi in buffaloes (bubalus bubalis) in assiut, egypt. | this study was performed for the purpose of investigating the prevalence and the species composition of sarcocystis spp. in buffaloes in assiut province, egypt. macroscopically we reported the infection of buffaloes with sarcocystis fusiformis, while microscopically three sarcocystis species (sarcocystis cruzi, sarcocystis levinei and sarcocystis hominis) cysts were recognized, and were differentiated by their morphological features using both histopathological sections and electron microscope s ... | 2014 | 25320487 |
| high prevalence of muscular sarcocystosis in cattle and water buffaloes from selangor, malaysia. | the prevalence of sarcocystosis in cattle and water buffaloes from peninsular malaysia was investigated in abattoirs in selangor state, february, 2011, to march, 2012. fresh muscle samples were collected from the tongue, heart, oesophagus, diaphragm and skeletal muscles of 102 cattle and 18 water buffaloes. each sample was initially screened by light microscopy and then fixed for further histopathological analysis. out of 120 animals examined, 49 (40.8%) harboured the microscopic type of sarcocy ... | 2013 | 24522140 |
| domestic cats (felis catus) are definitive hosts for sarcocystis sinensis from water buffaloes (bubalus bubalis). | the definitive hosts of sarcocystis sinensis in water buffaloes have hitherto been unknown, but the close similarity of this species to the cat-transmitted sarcocystis bovifelis in cattle suggested they were felids. in a previous study, two domestic cats were fed macroscopic sarcocysts of sarcocystis fusiformis contained within or dissected from the esophageal muscles of water buffaloes, while no microscopic sarcocysts of s. sinensis were noticed. both cats started shedding small numbers of spor ... | 2016 | 27075117 |
| molecular characterisation of three regions of the nuclear ribosomal dna unit and the mitochondrial cox1 gene of sarcocystis fusiformis from water buffaloes (bubalus bubalis) in egypt. | a total of 33 macroscopically visible (3-11 × 1-5 mm) sarcocysts of sarcocystis fusiformis were excised from the oesophagus of 12 freshly slaughtered water buffalos in giza, egypt. genomic dna was extracted from the sarcocysts, and all isolates were characterised at the mitochondrial cytochrome c oxidase subunit i (cox1) gene through pcr amplification and direct sequencing, whereas a few selected isolates were characterised at the 18s and 28s ribosomal (r) rna genes and the internal transcribed ... | 2015 | 26051128 |
| redescription of sarcocystis fusiformis sarcocysts from the water buffalo (bubalus bubalis). | four valid species of sarcocystis have been reported from the water buffalo (bubalus bubalis): sarcocystis fusiformis, sarcocystis buffalonis, sarcocystis levinei and sarcocystis dubeyi. here, we redescribe structure of s. fusiformis sarcocysts by scanning and transmission electron microscopy (sem, tem). twenty-one macroscopic sarcocysts from oesophagus of the water buffalo in egypt were examined by light microscopy, sem and tem. the sarcocyst wall was up to 9 μm thick, depending on the section ... | 2015 | 25111676 |
| the resurrection of a species: sarcocystis bovifelis heydorn et al., 1975 is distinct from the current sarcocystis hirsuta in cattle and morphologically indistinguishable from sarcocystis sinensis in water buffaloes. | in the mid-1970s, it was established through transmission experiments and ultrastructural studies of sarcocysts by transmission electron microscopy (tem) that cattle was the intermediate host of three sarcocystis spp. using dogs, cats and humans, respectively, as definitive hosts. the cat-transmitted species with microscopic sarcocysts was initially named sarcocystis bovifelis, but it was soon renamed sarcocystis hirsuta, since it was considered to be identical with a previously named species. i ... | 2016 | 26462803 |
| prevalence and first molecular identification of sarcocystis species in cattle and water buffaloes in india. | the importance of sarcocystis hominis in causing zoonotic infections is well known. recently, s. hominis like cysts have been reported from water buffalo in china. previous studies indicate prevalence of sarcocystis species in bovine populations in india but molecular evidence is required for proper species differentiation. we examined two hundred and ninety six cardiac tissue samples of indian water buffaloes and cattle from northern and western parts of the country. tissues were examined for s ... | 2016 | 27447215 |
| sarcocystis rommeli, n. sp. (apicomplexa: sarcocystidae) from cattle (bos taurus) and its differentiation from sarcocystis hominis. | cattle (bos taurus) are intermediate hosts for three named species of sarcocystis, s. cruzi, s. hirsuta, and s. hominis. recently, a fourth species was identified and named s. sinensis. however, s. sinensis originally named a species of sarcocystis in water buffalo (bubalus bubalis) in china. based on unverifiable evidence, it was suggested that the same parasite infects cattle. in addition, s. sinensis was recently declared as nomen nudum because its naming violated the rules of international c ... | 2016 | 26111603 |
| identity of sarcocystis species of the water buffalo (bubalus bubalis) and cattle (bos taurus) and the suppression of sarcocystis sinensis as a nomen nudum. | there are uncertainties concerning the identity and host species specificity of sarcocystis species of the water buffalo (bubalus bubalis) and cattle (bos taurus). currently, in cattle three species are recognized with known endogenous stages, viz.: s. cruzi (with canine definitive host), s. hirsuta (feline definitive host), and s. hominis (primate definitive host). recently, a fourth sarcocystis species with an unknown life cycle has been reported from cattle. in the water buffalo, four species ... | 2014 | 25034134 |
| combined il-12 plasmid and recombinant sjgst enhance the protective and anti-pathology effect of sjgst dna vaccine against schistosoma japonicum. | schistosomiasis is listed as one of most important tropical diseases and more than 200 million people are estimated to be infected. development of a vaccine is thought to be the most effective way to control this disease. recombinant 26-kda glutathione s-transferase (rsjgst) has previously been reported to achieve a worm reduction rate of 42-44%. to improve the efficiency of the vaccine against schistosoma japonicum, we immunized mice with a combination of pcdna vector-encoded 26-kda sjgst (pcdn ... | 2016 | 26891172 |
| comparative analysis of transcriptional profiles of adult schistosoma japonicum from different laboratory animals and the natural host, water buffalo. | schistosomiasis is one of the most widely distributed parasitic diseases in the world. schistosoma japonicum, a zoonotic parasite with a wide range of mammalian hosts, is one of the major pathogens of this disease. although numerous studies on schistosomiasis japonica have been performed using laboratory animal models, systematic comparative analysis of whole-genome expression profiles in parasites from different laboratory animals and nature mammalian hosts is lacking to date. | 2015 | 26285138 |
| screening diagnostic candidates for schistosomiasis from tegument proteins of adult schistosoma japonicum using an immunoproteomic approach. | schistosomiasis is one of the world's most prevalent zoonotic diseases and a serious worldwide public health problem. since the tegument (tg) of schistosoma japonicum is in direct contact with the host and induces a host immune response against infection, the identification of immune response target molecules in the schistosome tg is crucial for screening diagnostic antigens for this disease. | 2015 | 25706299 |
| microarray analysis of gene expression profiles of schistosoma japonicum derived from less-susceptible host water buffalo and susceptible host goat. | water buffalo and goats are natural hosts for s. japonicum in endemic areas of china. the susceptibility of these two hosts to schistosome infection is different, as water buffalo are less conducive to s. japonicum growth and development. to identify genes that may affect schistosome development and survival, we compared gene expression profiles of schistosomes derived from these two natural hosts using high-throughput microarray technology. | 2013 | 23940568 |
| identifying host species driving transmission of schistosomiasis japonica, a multihost parasite system, in china. | understanding disease transmission dynamics in multihost parasite systems is a research priority for control and potential elimination of many infectious diseases. in china, despite decades of multifaceted control efforts against schistosomiasis, the indirectly transmitted helminth schistosoma japonicum remains endemic, partly because of the presence of zoonotic reservoirs. we used mathematical modeling and conceptual frameworks of multihost transmission ecology to assess the relative importance ... | 2013 | 23798418 |
| a novel procedure for precise quantification of schistosoma japonicum eggs in bovine feces. | schistosomiasis japonica is a zoonosis with a number of mammalian species acting as reservoir hosts, including water buffaloes which can contribute up to 75% to human transmission in the people's republic of china. determining prevalence and intensity of schistosoma japonicum in mammalian hosts is important for calculating transmission rates and determining environmental contamination. a new procedure, the formalin-ethyl acetate sedimentation-digestion (fea-sd) technique, for increased visualiza ... | 2012 | 23166847 |
| ultrastructural observation and gene expression profiling of schistosoma japonicum derived from two natural reservoir hosts, water buffalo and yellow cattle. | water buffalo and yellow cattle are the two of the most important natural reservoir hosts for schistosoma japonicum in endemic areas of china, although their susceptibility differs, with water buffalo being less conducive to the growth and development of s. japonicum. results from the current study show that the general morphology and ultrastructure of adult schistosomes derived from the two hosts also differed. using high-throughput microarray technology, we also compared the gene expression pr ... | 2012 | 23110087 |
| [screening and verification on characteristic differentially expressed genes of schistosoma japonicum from three reservoir hosts]. | to get the characteristic differentially expressed genes of schistosoma japonicum from three important reservoir hosts: yellow cattle, water buffalo and goat, so as to find the genetic markers to identify the various sources of the parasite reservoir hosts. | 2012 | 23012949 |
| efficacy of a polyvalent mastitis vaccine against staphylococcus aureus on a dairy mediterranean buffalo farm: results of two clinical field trials. | in the last years the knowledges on mediterranean buffalo (mb) mastitis is remarkably improving, nevertheless the attention has been never focused on vaccination as preventive strategy for the control of mastitis in these ruminates. the aim of the current study was to assess clinical efficacy over time of two different preventive vaccination protocols against s. aureus mastitis, in primiparous mb.vaccinated (vg) and not-vaccinated (n-vg) groups, of 30 mb each one, were selected from two differen ... | 2017 | 28103866 |
| clinical outcomes and molecular genotyping of staphylococcus aureus isolated from milk samples of dairy primiparous mediterranean buffaloes (bubalus bubalis). | staphylococcus aureus is one of the most important pathogens causing mastitis in dairy cows and in mediterranean buffaloes. genotype b (gtb) is contagious in dairy cows and may occur in up to 87% of cows of a dairy herd. it was the aim of this study to evaluate genotypes present, clinical outcomes, and prevalence of staph. aureus in milk samples of primiparous mediterranean dairy buffaloes. two hundred composite milk samples originating from 40 primiparous buffaloes were collected from may to ju ... | 2014 | 25459906 |
| identification of virulence factors in 16s-23s rrna intergenic spacer genotyped staphylococcus aureus isolated from water buffaloes and small ruminants. | staphylococcus aureus is an important human and animal pathogen, and is regarded as an important cause of intramammary infection (imi) in ruminants. staphylococcus aureus genetic variability and virulence factors have been well studied in veterinary medicine, especially in cows as support for control and management of imi. the aim of the present study was to genotype 71 staph. aureus isolates from the bulk tank and foremilk of water buffaloes (n=40) and from udder tissue (n=7) and foremilk (n=24 ... | 2013 | 24140323 |
| short communication: role of streptococcus pluranimalium in mediterranean buffaloes (bubalus bubalis) with different udder health statuses. | the aims of the current study were to describe presence and clinical role over time of streptococcus pluranimalium isolated in milk samples of mediterranean buffalo (mb). two hundred composite milk samples originating from 40 primiparous mb were collected at 10, 30, 60, 90, and 150d in milk (dim) and from 20 pluriparous mb at 77 to 120 dim. milk samples were used for analysis of somatic cell counts, bacteriological cultures, and identification (matrix-assisted laser desorption/ionization time-of ... | 2016 | 26805969 |
| no change in mrna expression of immune-related genes in peripheral blood mononuclear cells challenged with theileria annulata in murrah buffalo (bubalus bubalis). | water buffaloes (bubalus bubalis) act as carrier to theileria annulata and show less clinical sign of tropical theileriosis as compared to indigenous and exotic cattle. differential expression of immune-related genes such as major histocompatibility complex, class ii, dq alpha 1 (mhc-dqα), signal-regulatory protein alpha (sirpa), prion protein (prnp), toll-like receptor 10 (tlr10), c-musculoaponeurotic fibrosarcoma oncogene homolog (cmaf) and v-maf avian musculoaponeurotic fibrosarcoma oncogene ... | 2016 | 26997138 |
| serum acute phase proteins in control and theileria annulata infected water buffaloes (bubalus bubalis). | this study was carried out to ascertain the changes in acute phase proteins (apps) and pro-inflammatory cytokines in theileria annulata infected water buffalo. thirty infected water buffaloes and 20 parasitologically free were used. in the present study there was significant (p ≤ 0.05) increase in haptoglobin (hp), serum amyloid a (saa), ceruloplasmin, α1-acid glycoprotein (agp) and fibrinogen levels (2.18 ± 0.29 g/l, 156.58 ± 3.48 mg/l, 31.23 ± 1.25mg/dl, 370.23 ± 33.21 mg/l and 16.17 ± 1.18 g/ ... | 2012 | 22785130 |
| molecular characterisation of theileria orientalis in imported and native bovines from pakistan. | the epidemiological aspects of theileria orientalis in pakistan are unknown; therefore, investigations using sensitive and precise molecular techniques are required. this study reports the first molecular characterisation of t. orientalis detected from imported (bos taurus) and native cattle (bos indicus×bos taurus) and buffaloes (bubalus bubalis) selected from four districts of punjab, pakistan. dna samples from blood (n=246) were extracted and tested using conventional pcr utilising the major ... | 2017 | 27838527 |
| fatal theileria orientalis n2 genotype infection among asian water buffaloes (bubalus bubalis) in a commercial dairy farm in kerala, india. | fifteen dairy buffaloes of a farm in the state of kerala, india developed fatal oriental theileriosis within 2 months of their procurement. typical piroplasms of theileria orientalis were observed in the erythrocytes of all affected animals by giemsa-leishman staining of blood smears. case fatality rate was 87·5% (seven out of eight) in the clinically progressed cases. therapeutic management with anti-theilerial drugs buparvaquone and oxytetracycline led to recovery of seven other animals in les ... | 2016 | 26522773 |
| investigation of anti-toxoplasma gondii antibodies in water buffaloes (bubalus bubalis) in samsun and afyon provinces. | the present study was conducted to investigate the seroprevalence of toxoplasma gondii infection in water buffaloes. | 2014 | 25732878 |
| seroprevalence of toxoplasma gondii infection in water buffaloes (bubalus bubalis) in veracruz state, mexico and its association with climatic factors. | infection with toxoplasma gondii in water buffaloes (bubalus bubalis) is of epidemiological importance because of the risk for transmission to humans. we sought to determine the seroprevalence of t. gondii infection in 339 water buffaloes in veracruz state, mexico using the modified agglutination test (mat, cut off 1:25). seroprevalence association with general characteristics of buffaloes and their environment was also investigated. | 2014 | 25267265 |
| trypanosoma vivax in water buffalo of the venezuelan llanos: an unusual outbreak of wasting disease in an endemic area of typically asymptomatic infections. | trypanosoma vivax has been associated with asymptomatic infections in african and south american buffalo. in this study, t. vivax was analyzed in water buffalo (bubalus bubalis) from venezuela in a molecular survey involving 293 blood samples collected from 2006 to 2015 across the llanos region. results demonstrated constant infections (average 23%) during the years analyzed. in general, animals were healthy carriers of t. vivax with low levels of parasitemia and were diagnosed exclusively by tv ... | 2016 | 27884441 |
| seroprevalence of trypanosoma evansi infection in water buffaloes from the mountainous region of north vietnam and effectiveness of trypanocidal drug treatment. | water buffalo (wb) is an important domestic animal in vietnam. this study utilized a card agglutination test to investigate seroprevalence of surra in wb population. sera were collected from 585 wb from 4 different regions in cao bang and thai nguyen provinces. among them, 131 samples (22.4%) were positive for surra. the highest prevalence (24.6%) was found among 3 to 5 years old wb. buffaloes less than 3 years old had the lowest prevalence (15.6%). among 27 abortion cases, 9 wb (33.3%) were sur ... | 2013 | 23665513 |
| molecular characterization of trypanosoma evansi isolates from water buffaloes (bubalus bubalis) in the philippines. | trypanosoma evansi infection in the philippines is frequently reported to affect the country's livestock, particularly, the buffaloes. to assess the prevalence and intraspecific diversity of t. evansi in the country, blood samples from water buffaloes in different geographical regions were collected during an outbreak. t. evansi was detected in all 79 animals tested using pcr targeting the rotat 1.2 vsg gene. sequencing of the rdna complete internal transcribed spacer (its) region including the ... | 2013 | 23377905 |
| genetic diversity in trypanosoma theileri from sri lankan cattle and water buffaloes. | trypanosoma theileri is a hemoprotozoan parasite that infects various ruminant species. we investigated the epidemiology of this parasite among cattle and water buffalo populations bred in sri lanka, using a diagnostic pcr assay based on the cathepsin l-like protein (catl) gene. blood dna samples sourced from cattle (n=316) and water buffaloes (n=320) bred in different geographical areas of sri lanka were pcr screened for t. theileri. parasite dna was detected in cattle and water buffaloes alike ... | 2015 | 25554063 |
| can rare positive interactions become common when large carnivores consume livestock? | livestock populations in protected areas are viewed negatively because of their interaction with native ungulates through direct competition for food resources. however, livestock and native prey can also interact indirectly through their shared predator. indirect interactions between two prey species occur when one prey modifies either the functional or numerical responses of a shared predator. this interaction is often manifested as negative effects (apparent competition) on one or both prey s ... | 2012 | 22624309 |
| the efficacy and safety of alphacypermethrin as a pour-on treatment for water buffalo (bubalus bubalis) infested with haematopinus tuberculatus (phthiraptera: haematopinidae). | the sucking louse haematopinus tuberculatus (burmeister 1839) is an ectoparasite of buffaloes, cattle, camels, and american bison. alphacypermethrin (acyp) is a pyrethroid insecticide commonly used to control arthropods of veterinary and public health interest. therapeutics, such as antiparasitic compounds, is often administered to buffaloes based on dosage and intervals recommended for cattle because very few drugs have buffalo-specific label indications. a trial was conducted on 20 louse-infes ... | 2013 | 23733232 |
| polymorphism and haplotype structure in river buffalo (bubalus bubalis) toll-like receptor 5 (tlr5) coding sequence. | most of the 160 million river buffalo in the world are in asia where they are used extensively, both as a food source and for draught power. only recently have investigations begun exploring the buffalo genome for variation that might influence health and productivity of these economically important animals. this paper describes the sequence variability of the toll-like receptor 5 (tlr5) gene, which recognizes bacterial flagellin and is a key player in the immune system. tlr5 is comprised of a s ... | 2012 | 22537062 |
| the prion protein gene polymorphisms associated with bovine spongiform encephalopathy susceptibility differ significantly between cattle and buffalo. | prion protein, encoded by the prion protein gene (prnp), plays a crucial role in the pathogenesis of transmissible spongiform encephalopathies (tses). several polymorphisms within the prnp are known to be associated with influencing bovine spongiform encephalopathy (bse) susceptibility in cattle, namely two insertion/deletion (indel) polymorphisms (a 23-bp indel in the putative promoter and a 12-bp indel in intron 1), the number of octapeptide repeats (octarepeats) present in coding sequence (cd ... | 2015 | 26319996 |
| comparative sequence analysis in the exon 5 of growth hormone gene in the various livestock species of india. | the aim of the study was to identify genetic polymorphism in growth hormone (gh) gene locus of six different livestock species using pcr-direct dna sequencing method. in exon 5 of gh gene, 10 snps variants were identified in all livestock species studied, namely bubalus bubalis, bos indicus, bos frontalis, bos grunniens, ovis aries, and capra hircus. four snps were observed in bubalus bubalis, two snps in bos indicus, one snp in ovis aries, and three snps in capra hircus. no changes were observe ... | 2014 | 24299185 |
| [polymorphisms of inhibin α gene exon 1 in buffalo (bubalus bubalis), gayal (bos frontalis) and yak (bos grunniens)]. | to elucidate the genetic characteristics of the bovine inhibin α subunit (inha) gene, the polymorphisms in exon 1 of inha and its bilateral sequences were assayed using pcr with direct sequencing in buffalo, gayal and yak. a comparative analysis was conducted by pooled the results in this study with the published data of inha on some mammals including some bovine species together. a synonymous substitution c.73c>a was identified in exon 1 of inha for buffalo, which results in identical encoding ... | 2012 | 22855448 |
| the molecular phylogenetic signature of bali cattle revealed by maternal and paternal markers. | bali cattle is a domestic cattle breed that can be found in malaysia. it is a domestic cattle that was purely derived from a domestication event in banteng (bos javanicus) around 3,500 bc in indonesia. this research was conducted to portray the phylogenetic relationships of the bali cattle with other cattle species in malaysia based on maternal and paternal lineage. we analyzed the cytochrome c oxidase i (coi) mitochondrial gene and sry of y chromosome obtained from five species of the bos genus ... | 2013 | 23686165 |
| sequence characterization, polymorphism, and tissue expression profile of an effector immediate-early gene: activity-regulated cytoskeletal associated protein gene (arc/arg3.1) in swamp and river buffalo. | the activity-regulated cytoskeletal associated protein (arc/arg3.1) has been implicated in experience-dependent synaptic plasticity and memory formation. however, information regarding its coding gene in buffalo remains scarce. in this study, the full-length of arc/arg3.1 was isolated and characterized (accession no. jx491649) and genetic variations of six river buffalo and eight swamp buffalo were investigated. a tissue expression profile was obtained using semi-quantitative reverse transcripti ... | 2014 | 24737478 |
| dna-based authentication method for detection of yak (bos grunniens) in meat products. | a taqman probe real-time pcr method was developed for rapid detection of yak component in raw and cooked meat products. specific primers and taqman probes of yak (bos grunniens) were designed in the cytochrome b gene. the specificity of the method was evaluated using pure meat of eight yak breeds (jiulong, qinghai plateau, maiwa, gannan, bazhou, sibu, zhongdian, and jiali) samples and nine non-bos grunniens animals (sheep, goat, pig, chicken, cattle, water buffalo, donkey, horse, and rabbit). dn ... | 2013 | 23513969 |
| cryopreservation of nili-ravi buffalo bull sperm in cryodiluent supplemented with lolium perenne protein preparations. | semen from the nili-ravi buffalo bull, bubalus bubalis, shows poor survival after freeze storage compared to bovine (bos taurus and bos indicus) semen. freeze-susceptibility distinctions in these two genera have been attributed to differences in sperm membranes. | 2017 | 28376139 |
| differential expression of isg 15 mrna in peripheral blood mononuclear cells of nulliparous and multiparous pregnant versus non-pregnant bos indicus cattle. | embryonic mortality is found to be the main source of reproductive wastage in domestic ruminants. many genes are involved in the growth and development of the embryo, and the interferon-stimulated gene 15 (isg 15) is one of the major gene stimulated by interferon tau, the maternal recognition of pregnancy signal in ruminants. in this study, both genomic and cdna sequences of isg 15 from bos indicus (deoni breed) were amplified and characterized. the genomic sequence of deoni isg 15 exhibited 99% ... | 2017 | 27766692 |
| challenges for beef production in smallholder communities with low reproductive management skills: a case study from northern lao pdr. | improved large ruminant productivity is increasingly acknowledged as a pathway for the alleviation of rural poverty and food insecurity in smallholder communities in southeast asia; yet, in much of laos, bovine reproductive management is practically absent. large ruminant reproduction skills were studied, using face-to-face surveys (n=60) of the knowledge, attitudes, and practices (kap) of farmers, plus an extension of an examination of parameters of reproductive efficiency (n = 1786 cattle and ... | 2017 | 27718106 |
| expression profiling of major heat shock protein genes during different seasons in cattle (bos indicus) and buffalo (bubalus bubalis) under tropical climatic condition. | heat shock proteins consist of highly conserved stress proteins, expressed in response to stress and play crucial roles in environmental stress tolerance and adaptation. the present study was conducted to identify major types of genes under the hsp70 family and other hsps and to evaluate their expression pattern in sahiwal and tharparkar breeds of zebu cattle (bos indicus) and murrah buffalo (bubalus bubalis) with respect to different seasons. quantitative real time polymerase chain reaction was ... | 2015 | 25965018 |
| generation of bovine (bos indicus) and buffalo (bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality. | adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. in this study, we report on the isolation and characterization of mesenchymal stem cells (msc) derived from bovine and buffalo adipose tissue. cells isolated using enzymatic digestion of bovine and buffalo adipose-tissue biopsy samples were grown in vitro for at least 15 passages, verifyi ... | 2015 | 25729935 |
| improving smallholder food security through investigations of carcass composition and beef marketing of buffalo and cattle in northern lao pdr. | this study determined the carcass composition of lao indigenous buffalo (bubalus bubalis) and cattle (bos indicus), then examined trends in bovine meat marketing following review of records of beef production and prices in the two major cities of luang prabang (lpb) and xieng khoung (xk) provinces in northern laos. samples from 41 buffalo and 81 cattle (n = 122) were collected from animals slaughtered in may-june 2014, with live weights, carcass weights and other carcass-related variables collec ... | 2015 | 25700673 |
| comparative expression profiling of insulin-like growth factor binding protein-5 in milk of bos indicus and bubalus bubalis during lactation. | insulin-like growth factor binding protein-5 (igfbp-5) is a key molecule in mammary gland development, which facilitates the removal of mammary epithelial cells (mecs) by apoptosis that takes place during remodeling of the mammary gland during involution. igfbp-5 binds with igfs for their bioavailability. igfbp-5 has been reported to perform pleiotropic roles such as cellular apoptosis, proliferation and differentiation. to understand the role of igfbp-5 during lactation and clinical mastitis, e ... | 2015 | 25491373 |
| the interval between the emergence of pharmacologically synchronized ovarian follicular waves and ovum pickup does not significantly affect in vitro embryo production in bos indicus, bos taurus, and bubalus bubalis. | the aim of the present study was to determine the optimal phase of the follicular wave to perform ovum pickup (opu) for in vitro embryo production (ivep) in various genetic groups. for this purpose, 27 heifers-nine bos taurus (holstein), nine bos indicus (nelore), and nine bubalus bubalis (mediterranean)-were maintained under the same nutritional, management, and environmental conditions. heifers within each genetic group were submitted to six consecutive opu trials with 14-day intersession inte ... | 2015 | 25447149 |
| anti-mullerian hormone concentration and antral ovarian follicle population in murrah heifers compared to holstein and gyr kept under the same management. | this study was performed to evaluate plasma concentrations of anti-mullerian hormone (amh) and the ovarian antral follicle population (afp) in different genetic groups. cyclic heifers (13 bubalus bubalis [murrah]; 15 bos taurus [holstein] and 10 bos indicus [gyr]) were maintained under the same management and were synchronized with two doses of 150 μg im d-cloprostenol administered 14 days apart. after the second d-cloprostenol treatment, heifers had their ovaries scanned daily by ultrasound to ... | 2014 | 25291989 |
| handling and welfare of bovine livestock at local abattoirs in bangladesh. | the world organization for animal health (oie) allows rope casting and the tying of legs for nonhuman animal slaughter without stunning. the handling and welfare of bovine livestock (bos indicus and bubalus bubalis) were studied in 8 local abattoirs in 5 districts of bangladesh. a total of 302 animals were evaluated. at the local abattoirs, approximately 1/3 of the cattle and water buffalo were either emaciated or injured/sick. the size and vigor of the animals determined the casting method. sma ... | 2014 | 24766081 |
| evaluation of hemostatic activity of latex from three euphorbiaceae species. | latices from several plant species of euphorbiaceae family have been traditionally applied over fresh cuts to stop bleeding and subsequently applied over wounds to enhance healing process. the latex arrested bleeding from fresh wounds by reducing bleeding and whole blood coagulation time which are important indices of hemostatic activity. it has been accepted that hemostatic activity is due to the proteolytic fraction of plant latices. thus, the present study aimed to assess the clot inducing pr ... | 2014 | 24291032 |
| novel polymorphisms in utr and coding region of inducible heat shock protein 70.1 gene in tropically adapted indian zebu cattle (bos indicus) and riverine buffalo (bubalus bubalis). | due to evolutionary divergence, cattle (taurine, and indicine) and buffalo are speculated to have different responses to heat stress condition. variation in candidate genes associated with a heat-shock response may provide an insight into the dissimilarity and suggest targets for intervention. the present work was undertaken to characterize one of the inducible heat shock protein genes promoter and coding regions in diverse breeds of indian zebu cattle and buffaloes. the genomic dna from a panel ... | 2013 | 23792016 |
| characterization of the exonic regions of the jy-1 gene in zebu cattle and buffaloes. | protein jy-1 is an oocyte-specific protein that plays an important regulatory role in the granulosa cell layer and during the early embryo development stages. it is the first specific protein of maternal origin discovered in a single-ovulating species. in this study, the exon regions of the jy-1 gene were characterized by sequencing in 20 unrelated cattle (bos taurus indicus) and 20 unrelated buffaloes (bubalus bubalis). eighteen polymorphisms were detected in cattle and 10 polymorphisms in buff ... | 2013 | 23714232 |
| sequence and topological characterization of toll-like receptor 8 gene of indian riverine buffalo (bubalus bubalis). | in this study, buffalo (bubalus bubalis) toll-like receptor 8 (tlr8) gene has been characterized by sequence analysis and detecting polymorphism. complete orf of buffalo tlr8 gene was amplified using the rna isolated from spleen tissue, which was found to be 3,102 nucleotides long encoding a 1,033 amino acid protein. buffalo tlr8 had 10 nucleotide changes as compared to other livestock species resulting in six unique amino acid changes, four of them lying within leucine-rich repeat (lrr) domains ... | 2013 | 22622670 |
| lumpy skin disease in cattle: frequency of occurrence in a dairy farm and a preliminary assessment of its possible impact on egyptian buffaloes. | lumpy skin disease (lsd) is an endemic infectious disease of cattle in egypt. this survey aimed to define the prevalence of clinical and sub-clinical lsd virus (lsdv) infection among cattle and investigate their contact with water buffaloes (bubalus bubalis) in order to improve the understanding of lsd epidemiology. cattle and buffalo were examined owing to the appearance of skin lesions. because clinical signs were consistent with lsdv infection, samples from cattle in a non-grazing dairy farm ... | 2017 | 28397517 |
| complement component 3: characterization and association with mastitis resistance in egyptian water buffalo and cattle. | mastitis is an infectious disease of the mammary gland that leads to reduced milk production and change in milk composition. complement component c3 plays a major role as a central molecule of the complement cascade involving in killing of microorganisms, either directly or in cooperation with phagocytic cells. c3 cdna were isolated, from egyptian buffalo and cattle, sequenced and characterized. the c3 cdna sequences of buffalo and cattle consist of 5025 and 5019 bp, respectively. buffalo and ca ... | 2017 | 28360391 |
| molecular cloning, identification, and expression patterns of myostatin gene in water buffalo (bubalus bubalis). | myostatin (mstn), also named growth differentiation factor 8 (gdf8), is a transforming growth factor-β (tgf-β) family member with a key role in the negative regulation of skeletal muscle growth. however, its role in ovarian folliculogenesis remains unclear. to provide us with a basis for understanding this role, we cloned mstn and examined its expression patterns in water buffalo (bubalus bubalis). the complete orf of the water buffalo mstn gene is 1,128 nucleotides, which encode a 375 amino aci ... | 2017 | 28355117 |
| identification of single nucleotide polymorphism in protein phosphatase 1 regulatory subunit 11 gene in murrah bulls. | this study was conducted with the objective to identify single nucleotide polymorphism (snp) in protein phosphatase 1 regulatory subunit 11 (ppp1r11) gene in murrah bulls. | 2017 | 28344410 |
| comparative study of the gut microbiome potentially related to milk protein in murrah buffaloes (bubalus bubalis) and chinese holstein cattle. | previous studies suggested a close relationship between ruminant gut microbes and the mammary gland. in this study, shotgun metagenomic sequencing was used to reveal the differences in the intestinal microbiome potentially related to milk components in murrah buffaloes and chinese holstein cattle. a pcoa based on the weighted unifrac distances showed an apparent clustering pattern in the structure of intestinal microbiota between buffalo and cattle. we could attribute the structural difference t ... | 2017 | 28176851 |
| comparative glycomics of fat globule membrane glycoconjugates from buffalo (bubalus bubalis) milk and colostrum. | the health-promoting effects of milk fat globule membrane (mfgm) glycoconjugates has attracted curiosity especially with regard to the challenges encountered to unravel the glycan complexities of mfgm glycoproteins and glycosphingolipids. in this context, we characterized glycans present in buffalo milk and colostrum fat globule membranes by matrix-assisted laser desorption/ionization time-of-flight (maldi-tof) ms analysis by adopting chemoselective glycoblotting technique. unlike human and bovi ... | 2017 | 28145111 |
| a brief and critical review on hydrofluorosis in diverse species of domestic animals in india. | india is one of the fluoride-endemic countries where the maximum numbers of ground or drinking water sources are naturally fluoridated. in india, a total of 23, out of 36 states and union territories have drinking water contaminated with fluoride in varying concentration. in the present scenario, especially in rural india, besides the surface waters (perennial ponds, dams, rivers, etc.), bore wells and hand pumps are the principal drinking water sources for domestic animals such as cattle (bos t ... | 2017 | 28144801 |
| identification of genetic variation in nod-like receptor 2 gene and influence of polymorphism on gene structure and function in buffalo (bubalus bubalis). | nucleotide-binding oligomerization domain (nod)-like receptor 2 is one of the important mediators of innate as well as adaptive immune response to microbial infections. in this study, nod-like receptor-2 was characterized by determining the full gene sequence and analyzing genetic diversity in indian buffaloes. sequence analysis of buffalo nod2 revealed 3042 nucleotides long orf, encoding 1013 amino acids from 12 exons. domain structure analysis indicated existence of 8 leucine-rich repeat (lrr) ... | 2017 | 28135669 |
| molecular characterization of hoxc8 gene and methylation status analysis of its exon 1 associated with the length of cashmere fiber in liaoning cashmere goat. | homeobox protein hox-c8 (hoxc8) is a member of hox family. it is expressed in the dermal papilla of the skin and is thought to be associated with the hair inductive capacity of dermal papilla cells. in the present study, we isolated and characterized a full-length open reading frame of hoxc8 cdna from the skin tissue of liaoning cashmere goat, as well as, established a phylogenetic relationship of goat hoxc8 with that of other species. also, we investigated the effect of methylation status of ho ... | 2017 | 28093668 |
| molecular cloning, polymorphism, and functional activity of the bovine and water buffalo mx2 gene promoter region. | bovine mx2 gene sequences were already reported, but further information about the gene properties is not yet available. the objective of the current study was to elucidate the structural properties of the bovine mx2 gene mainly the promoter region and its possible functional role. if available, such information would help in assessing the functional properties of the gene, which was reported to confer antiviral action against recombinant vsv. | 2016 | 28066698 |
| exploration of luteinizing hormone in murrah buffalo (bubalus bubalis) urine: extended surge window opens door for estrus prediction. | estrus detection in buffaloes has been a major concern for decades, and lack of reliable methods affects their effective reproductive management. luteinizing hormone (lh) detection in urine is in practice for several mammals for timed insemination, whereas very few reports are available on buffalo urinary lh. the focus of this study is to detect the presence of lh in buffalo urine, quantitate variation in urinary lh during different estrous cycle phases and examine the duration of mid-cycle lh w ... | 2016 | 28011259 |
| protein modeling and molecular dynamics simulation of cloned regucalcin (rgn) gene from bubalus bubalis. | regucalcin (rgn), a calcium regulating protein having anti prolific, anti-apoptotic functions, plays important part in the biosynthesis of ascorbic acid. it is a highly conserved protein that has been reported from many tissue types of various vertebrate species. employing its effect of regulating enzyme activities through reaction with sulfhydryl group (-sh) and calcium, structural level study is believed to offer a better understanding of its binding properties along with information exploited ... | 2016 | 28000561 |
| regucalcin is widely distributed in the male reproductive tract and exerts a suppressive effect on in vitro sperm capacitation in the water buffalo (bubalus bubalis). | regucalcin is a multi-functional, calcium-binding protein with roles in calcium homeostasis, apoptosis, cell proliferation, and free radical neutralization. regucalcin is broadly expressed in the male reproductive organs of rat and bovine; here, we report its expression in the reproductive tract of male buffalo-especially in testis, epididymis, seminal vesicle, prostate, and bulbourethral gland of buffalo-as analyzed by real-time pcr, western blot, and immunolocalization. regucalcin degradation ... | 2017 | 27987309 |
| validation of a fast real-time pcr method to detect fraud and mislabeling in milk and dairy products. | fast real-time pcr taqman assays were developed and validated for species identification in dairy products. based on the amplification of 12s rrna and cytb partial genes of mitochondrial dna, the methods were demonstrated to be sensitive, fast, and species-specific for bos taurus, ovis aries, bubalus bubalis, and capra hircus. the limit of detection calculated was lower than 1%, and the efficiency was reported to be higher than 96% in every assay. an internal amplification control was used to de ... | 2017 | 27865504 |
| prevalence of antibodies against bubaline herpesvirus (buhv-1) among mediterranean water buffalo (bubalus bubalis) with implications in buffalo trade. | both bovine herpesvirus (bohv-1) and bubaline herpesvirus (buhv-1) have been reported to cross the species barrier. antibody seroconversion in glycoprotein e (ge) blocking elisa during buhv-1 infection has been documented. recent diagnostic efforts have focused on the development and application of discriminatory tests to distinguish between infections with bohv-1 and buhv-1. | 2016 | 27334411 |
| antibodies to bovine viral diarrhoea virus (bvdv) in water buffalo (bubalus bubalis) and cattle from the northern territory of australia. | farmed and feral water buffaloes (bubalus bubalis) populations often coexist with cattle in the northern territory of australia, but their level of exposure to bovine viral diarrhoea virus (bvdv) is unknown. | 2016 | 27785794 |
| hsfy and znf280by show copy number variations within 17 water buffalo populations. | recent transcriptomic analysis of the bovine y chromosome revealed abundant presence of multi-copy protein coding gene families on the male-specific region of the y chromosome (msy). copy number variations (cnvs) of several msy genes are closely related to semen quality and male reproduction in cattle. however, the cnvs of msy genes in water buffalo are largely unknown. therefore, this study aimed to investigate the cnvs of hsfy and znf280by of 298 buffaloes from 17 populations distributed in ch ... | 2017 | 27739082 |
| replacement of serum with ocular fluid for cryopreservation of immature testes. | cryopreservation of immature testis is a feasible approach for germplasm preservation of male animals. combinations of dimethyl sulfoxide (dmso) and foetal bovine serum (fbs) are used for testis cryopreservation. however, an alternative to fbs is needed, because fbs is expensive. buffalo ocular fluid (buof), a slaughter house by-product, could be an economical option. the objective of the present study was to assess whether buof can replace fbs for cryopreservation of immature mouse (mus musculu ... | 2016 | 27693391 |
| impact of heat stress on cellular and transcriptional adaptation of mammary epithelial cells in riverine buffalo (bubalus bubalis). | the present study aims to identify the heat responsive genes and biological pathways in heat stressed buffalo mammary epithelial cells (mecs). the primary mammary epithelial cells of riverine buffalo were exposed to thermal stress at 42°c for one hour. the cells were subsequently allowed to recover at 37°c and harvested at different time intervals (30 min to 48 h) along with control samples (un-stressed). in order to assess the impact of heat stress in buffalo mecs, several in-vitro cellular par ... | 2017 | 27682256 |
| corrigendum to "investigation into omocysteine, vitamin e and malondialdehyde as indicators of successful artificial insemination in synchronized buffalo cows (bubalus bubalis)" [res. vet. sci. volume 104, february 2016, pages 100-105]. | | 2016 | 27495365 |
| alginate encapsulation preserves the quality and fertilizing ability of mediterranean italian water buffalo (bubalus bubalis) and holstein friesian (bos taurus) spermatozoa after cryopreservation. | the use of artificial insemination (ai) in buffalo (bubalus bubalis) is limited by poor ovarian activity during the hot season, seasonal qualitative patterns in semen, low resistance of sperm cells in the female tract, difficulties in estrus detection, and variable estrus duration. although ai procedures are commonly used in bovine, use of ai has been limited in buffalo. in the zootechnical field, different studies have been conducted to develop techniques for improvement of fertilizing ability ... | 2017 | 27456772 |
| molecular epidemiology of pathogenic leptospira spp. among large ruminants in the philippines. | the extent of leptospira infection in large ruminants resulting to economic problems in livestock industry in a leptospirosis-endemic country like the philippines has not been extensively explored. therefore, we determined the prevalence and carrier status of leptospirosis in large ruminants using molecular techniques and assessed the risk factors of acquiring leptospirosis in these animals. water buffalo and cattle urine samples (n=831) collected from 21 farms during 2013-2015 were subjected to ... | 2016 | 27452879 |
| a novel selection signature in stearoyl-coenzyme a desaturase (scd) gene for enhanced milk fat content in bubalus bubalis. | modern molecular interventions are dynamic gears for breeding animals with superior genetic make-up. these scientific efforts lead us toward sustainable dairy herds with improved milk production in terms of yield and quality. many of candidate genes have been dissected at molecular level, and suitable genetic markers have been identified in cattle, but this work has not been validated in buffaloes so far. stearoyl-coenzyme a desaturase (scd) has been a potential candidate gene for fat content of ... | 2016 | 27307277 |