| the historical roots of high rates of infant death in aboriginal communities in canada in the early twentieth century: the case of fisher river, manitoba. | infant mortality is investigated for a period of thirty years at the beginning of the 20th century in the aboriginal nations community of fisher river, manitoba. infant mortality rates were generated from parish records of infant burials from the methodist mission at fisher river and later archived at the united church archives in winnipeg, man. the average infant mortality rate (imr) for the total period (1910-1939) was 249 per 1000 live births, an exceedingly high rate compared to modern imrs ... | 1999 | 10405019 |
| the effects of boron neutron capture irradiation on oral mucosa: evaluation using a rat tongue model. | the ventral surface of the tongue of male fisher 344 rats was used to evaluate the response of oral mucosa to boron neutron capture irradiation. three hours after i.p. injection of 700 mg/kg of the boron delivery agent p-boronophenylalanine (bpa), the boron concentrations in blood and tongue mucosal epithelium were approximately 21 and 23 microgram (10)b/g, respectively. the doses required to produce a 50% incidence of ulceration with x rays, the brookhaven medical research reactor thermal neutr ... | 1999 | 10409319 |
| multiple pathways of prostate carcinogenesis analyzed by using cultured cells isolated from rats treated with n-methyl-n-nitrosourea and testosterone. | treatment of rats with n-methyl-n-nitrosourea (mnu) and testosterone results in a high incidence of metastasizing dorsolateral prostate tumors. in previous studies, a high frequency (> or = 70%) of a g35 --> a transition mutation at the second position of codon 12 of the ki-ras oncogene was found in these tumors. this was confirmed in the study reported here, and the frequency of this mutation appeared similar in tumors induced in four different rat strains, regardless of differences in sensitiv ... | 1999 | 10411144 |
| prevalence of penicillin resistant and multi-drug resistant streptococcus pneumoniae at a children's hospital. | streptococcus pneumoniae isolated at children's hospital of wisconsin during the winter of 1994 to 1995 and the winter of 1996 to 1997 were tested for susceptibility to penicillin, cephalosporins and other potentially therapeutically useful antimicrobial agents to determine the prevalence of penicillin and multi-drug resistant isolates. during those years, the prevalence of s. pneumomiae not susceptible to penicillin was 27% and 28%, respectively, with 14% and 18%, respectively, of the respirato ... | 1999 | 10414218 |
| evidence for the cofactor role of human t-cell lymphotropic virus type 1 in mycosis fungoides and sézary syndrome. | the aetiology of mycosis fungoides (mf) and sézary syndrome (ss) is unknown. a pathogenic role for the human t-cell lymphotropic virus type 1 (htlv-1) has been suggested but remains controversial. we used an animal model to test the possibility that peripheral blood mononuclear cells (pbmc) obtained from mf patients harbour the htlv-1 virus which may be infective. the polymerase chain reaction (pcr) was used to detect htlv-1 proviral dna sequences in pbmc of 27 mf patients and one ss patient of ... | 1999 | 10417514 |
| pretreatment of human keratinocyte sheets with laminin 5 improves their grafting efficiency. | laminin 5 is essential in epithelial attachment to stromal tissues, suggesting that it might improve keratinocyte attachment in a variety of clinical situations. in this study, we examined the effect of exogenous laminin 5 upon the efficiency of transplantation of keratinocyte sheets in animal models. keratinocyte sheets were prepared according to the method of rheinwald & green (1975). purified laminin 5 was added to the sheets of group 1 (1.0 microg per cm2), dulbecco's modified eagle's medium ... | 1999 | 10417616 |
| enhanced uptake of [3h] spermidine by 9l rat brain tumors after direct intratumoral infusion of inhibitors of enzymes of the polyamine biosynthetic pathway. | we have been exploring the feasibility of delivering ionizing radiation to brain tumor cells by using tritium labeled polyamines. polyamines are taken up preferentially by dividing cells and form noncovalent bonds with dna. their uptake can be enhanced by drugs which deplete endogenous polyamines. to test this in vivo, 9l cells were implanted in the striatal region of the brain in male fisher 344 rats. osmotic pumps containing trace amounts of [3h] spermidine or [3h] putrescine with either diflu ... | 1999 | 10421069 |
| comparison and recovery of escherichia coli and thermotolerant coliforms in water with a chromogenic medium incubated at 41 and 44.5 degrees c. | this study compared the performance of a commercial chromogenic medium, chromagarecc (cecc), and cecc supplemented with sodium pyruvate (ceccp) with the membrane filtration lauryl sulfate-based medium (mlsa) for enumeration of escherichia coli and non-e. coli thermotolerant coliforms (kec). to establish that we could recover the maximum kec and e. coli population, we compared two incubation temperature regimens, 41 and 44.5 degrees c. statistical analysis by the fisher test of data did not demon ... | 1999 | 10427079 |
| met-rantes reduces vascular and tubular damage during acute renal transplant rejection: blocking monocyte arrest and recruitment. | chemokines are thought to contribute to the cellular infiltrate characteristic of renal transplant rejection. we show that met-rantes, a chemokine receptor antagonist, suppresses recruitment of inflammatory cells into renal allografts. in a renal transplant model (fisher rt1(lvl) rat kidney into lewis rt1(l) rat) where no additional immune suppressant was used, met-rantes-treated animals showed a significant reduction in vascular injury score (16.10 +/- 5.20 vs. 62.67 +/- 18.64) and tubular dama ... | 1999 | 10428761 |
| intraductal spread of invasive breast carcinoma has a positive correlation with c-erb b-2 overexpression and vascular invasion. | studies of the histologic characteristics and biologic behavior of the intraductal spread of breast carcinoma are critically important in that they may lead to the identification of a unique spread pattern rather than a noninvasive lesion. | 1999 | 10430252 |
| enhanced ca(2+) mobilization in airway smooth muscle contributes to airway hyperresponsiveness in an inbred strain of rat. | the mechanisms underlying airway hyperresponsiveness are still unknown but increased contractility of airway smooth muscle may play a role. this study sought to demonstrate a relationship between in vivo airway responsiveness and a number of measures of airway smooth muscle responsiveness ex vivo, including intracellular ca(2+) signaling, by comparing three inbred strains of rat with different degrees of airways responsiveness to methacholine. lewis, aci, and fisher strains of rat were character ... | 1999 | 10430712 |
| bacterial colonization of central airways after stenting. | airway stenting (as) is increasingly used in the management of obstructive lesions of the central airways. although retention of secretions and infection have been reported as complications of as, the microbiological consequences of as have not yet been evaluated. in this study, we prospectively performed protected specimen brush (psb) sampling of the airways, before and 3 to 4 wk after as, in 14 consecutive patients (65 +/- 17 yr), suffering from bronchial (5), extensive esophageal (2), thyroid ... | 1999 | 10430745 |
| transplantation of fetal liver tissue suspension into the spleens of adult syngenic rats: effects of various mitogens and cytotoxins on cytochrome p450 (p450) isoforms expression and on p450 mediated monooxygenase functions. | syngenic fetal liver tissue suspensions were transplanted into the spleens of adult male fisher 344 inbred rats. four months after surgery, transplant recipients and age matched control rats were treated with various mitogens (fluorene [fen], fluorenone [fon] and 2-acetylaminofluorene [aaf]) or cytotoxins (allyl alcohol [aal], bromobenzene [bbz] and carbon tetrachloride [ccl4]) or the respective solvents 24 or 48 hours before sacrifice. the expression of three cytochrome p450 (p450) isoforms, 1a ... | 1999 | 10445401 |
| feeding soybean resistant protein to rats raises fecal bile acid excretion but counteracts a deoxycholate-caused decrease in colonic aberrant crypt foci. | a high-molecular-weight fraction after removal of water-soluble peptides from proteinase-treated soybean protein isolate (referred to as hmf) was examined for its effect on preneoplastic lesions in the rat colon. for this purpose, male fisher-344 rats 7 wk old were divided into 8 groups (n = 5), of which 6 groups received 3 injections of azoxymethane (aom, 15 mg/kg of body weight) for 3 wk once a week, while all were fed hmf or casein diets supplemented with or without deoxycholic acid (dca) ove ... | 1999 | 10450559 |
| [experimental studies of the protective effect of ginkgo biloba extract (gbe) on cisplatin-induced toxicity in rats]. | cisplatin (cddp) is an effective antineoplastic agent in the treatment of solid malignant tumors. its clinical use, however, is limited because of various side effects including sensorineural hearing loss. several agents have been proposed to reduce these side effects. gbe has recently been reported to scavenge superoxide and hydroxyl radicals, resulting in a reduction of lipid peroxidation. gbe is expected to protect against cddp-induced toxicity because its generative mechanism is thought to b ... | 1999 | 10459293 |
| age-related changes in renal function, membrane protein metabolism, and na,k-atpase activity and abundance in hypokalemic f344 x bnf(1) rats. | potassium depletion is a common electrolyte abnormality in elderly humans, usually as a consequence of diuretic use or poor oral intake. hypokalemia is associated with a number of changes in renal function and an increase in some renal membrane transporters; its growth-promoting effect in young animals is well known. with aging, the renal adaptation to a number of challenges is often diminished. we hypothesized that aging is related to decreases in renal function, renal membrane protein metaboli ... | 1999 | 10460986 |
| effects of capsaicin on induction of c-jun proto-oncogene expression in fisher-344 rats by n-methyl-n'-nitro-n-nitrosoguanidine. | n-methyl-n'-nitro-n-nitrosoguanidine (mnng) is a potent inducer of cellular stress leading to chromosomal aberrations, point mutations, and cell death. to study the effect of capsaicin on c-jun expression when given with mnng to rats, fisher-344 rats that had been administered mnng were treated with capsaicin in their diet and organs were removed for measuring c-jun transcripts. we show that pre- or post-treatment of capsaicin relative to mnng administration up- or down-regulates (depending on t ... | 1999 | 10463771 |
| mitochondrial dna inherited variants are associated with successful aging and longevity in humans. | mitochondrial dna (mtdna) is characterized by high variability, maternal inheritance, and absence of recombination. studies of human populations have revealed ancestral associated polymorphisms whose combination defines groups of mtdna types (haplogroups) that are currently used to reconstruct human evolution lineages. we used such inherited mtdna markers to compare mtdna population pools between a sample of individuals selected for successful aging and longevity (212 subjects older than 100 yea ... | 1999 | 10463944 |
| ageing alters aortic antioxidant enzyme activities in fischer-344 rats. | oxidative stress imposed by reactive oxygen species is now believed to contribute to hypertension, atherosclerosis and ageing of the vasculature all involving a loss of relaxation. the antioxidant enzymes glutathione peroxidase, superoxide dismutase and catalase play a crucial role in defending against the ravages of oxidative stress. our purpose was to characterize age-related changes in glutathione peroxidase, superoxide dismutase and catalase in the rat aorta. aortas were extracted from seven ... | 1999 | 10468656 |
| reduced expression of the cdk inhibitor p27(kip1) in rat two-stage bladder carcinogenesis and its association with expression profiles of p21(waf1/cip1) and p53. | the cyclin-dependent kinase (cdk) inhibitor p27(kip1) exerts its growth suppressive effects by targeting the cyclin-cdk complexes. reduced protein levels of p27(kip1) have been reported in numerous human cancers and this has been attributed to increased degradation. however, few reports have addressed the significance of p27(kip1) expression in chemical carcinogenesis of rodents. in a rat two-stage urinary bladder carcinogenesis model, with n-butyl-n-(4-hydroxybutyl)nitrosamine (bbn) initiation ... | 1999 | 10469613 |
| alterations of tumour cells, stroma and apoptosis in rat prostatic adenocarcinoma following treatment with histamine, interleukin-2 and irradiation. | the present study was designed to determine whether il-2 and histamine alone, or in combination could modulate the effects of irradiation on dunning (r3327) rat adenocarcinomas at the cellular level. copenhagen x fisher rats carrying bilateral tumours in the flank were used. when the tumours had a median volume of 150 mm3, one group of rats was treated with histamine alone (4 mgkg-1 subcutaneously on week days), another group with interleukin-2 (il-2) alone (425 iu kg-1 continuous infusion) and ... | 1999 | 10470141 |
| immunohistochemical detection of hsp60-expression in human ovarian cancer. correlation with survival in a series of 247 patients. | in a previous pilot study, hsp60 expression at the transcriptional (mrna) level was shown to be a negative prognostic factor in ovarian cancer. the aim of this study was to determine hsp60-expression by means of immunohistochemistry and to correlate the results with survival in a large series of ovarian carcinoma patients with a closed follow-up. | 1999 | 10470162 |
| coordinate induction of peroxisomal acyl-coa oxidase and ucp-3 by dietary fish oil: a mechanism for decreased body fat deposition. | rats fed dietary fats rich in 20- and 22-carbon polyenoic fatty acids deposit less fat and expend more energy at rest than rats fed other types of fats. we hypothesized that this decrease in energetic efficiency was the product of: (a) enhanced peroxisomal fatty acid oxidation and/or (b) the up-regulation of genes encoding proteins that were involved with enhanced heat production, i.e. mitochondrial uncoupling proteins (ucp-2, ucp-3) and peroxisomal fatty acid oxidation proteins. two groups of m ... | 1999 | 10471120 |
| dimer dissociation and thermosensitivity kinetics of the saccharomyces cerevisiae and human tata binding proteins. | a kinetic analysis of dimer dissociation, tata dna binding, and thermal inactivation of the yeast saccharomyces cerevisiae and human tata binding proteins (tbp) was conducted. we find that yeast tbp dimers, like human tbp dimers, are slow to dissociate in vitro (t(1/2) approximately 20 min). mild mutations in the crystallographic dimer interface accelerate the rate of dimer dissociation, whereas severe mutations prevent dimerization. in the presence of excess tata dna, which measures the entire ... | 1999 | 10471284 |
| genotoxic activity of chlorohydroxyfuranones in the microscale micronucleus test on mouse lymphoma cells and the unscheduled dna synthesis assay in rat hepatocytes. | chlorohydroxyfuranones (chfs) are mutagenic disinfection by-products found in chlorine-treated drinking water. in the current study, the genotoxicity of four chfs, 3,4-dichloro-5-hydroxy-2(5h)-furanone (mca), 3-chloro-4-methyl-5-hydroxy-2(5h)-furanone (mcf), 3-chloro-4-(chloromethyl)-5-hydroxy-2(5h)-furanone (cmcf) and 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5h)-furanone (mx), was determined. two in vitro assays, the microscale micronucleus assay on l5178y mouse lymphoma cells and the unschedule ... | 1999 | 10473648 |
| t-cell mediated autoimmunity to the insulinoma-associated protein 2 islet tyrosine phosphatase in type 1 diabetes mellitus. | the target molecules of the t-cell response in type 1 diabetes, despite their pathogenic importance, remain largely uncharacterized, especially in humans. interestingly, molecules such as insulin and glutamic acid decarboxylase (gad) have been shown to be a target not only of autoantibodies, but also of autoreactive t-lymphocytes both in man and in the non-obese diabetic (nod) mouse. in the present study we aimed to determine the existence of a specific t-cell response towards the insulinoma-ass ... | 1999 | 10474125 |
| [the cytoradiography of the dna and proteins in different types pf cells after the irradiation of rats with decimeter waves]. | microwave (2450 mhz, 1 mw/cm2, continuous regime) influence on dna and protein synthesis in bone marrow, testes and liver cells of fisher-line rats at early stage of formation of defence-adaptation reactions was studied. cytoradiographic analysis of tissue smears and prints using 3h-thimidine and 35s-methionine was made. type of reaction to microwave irradiation depends on ploidy, rate of differentiation and functional activity of cells. | 1999 | 10474861 |
| heightened ige response to mite antigens in inflammatory neuropathies. | in order to clarify the ige response to common environmental antigens, we measured the serum total ige and allergen-specific ige in 50 patients with guillain-barré syndrome (gbs), nine patients with fisher syndrome (fs), 14 patients with chronic inflammatory demyelinating polyneuropathy (cidp), 18 patients with mononeuritis multiplex (mnm), 43 patients with neurodegenerative disorders and 82 healthy controls by elisa. the total ige level was significantly higher in patients with gbs (median = 13 ... | 1999 | 10475098 |
| new method for detection of heart allograft rejection: validation of sensitivity and reliability in a rat heterotopic allograft model. | patients with inflammatory heart muscle diseases would benefit from a safe, convenient, rapidly performed diagnostic technique with real-time results not involving tissue removal. we have performed a detailed evaluation of detection of heart allograft rejection by autofluorescence in a heterotopic abdominal rat heart allograft model ex vivo. | 1999 | 10484546 |
| characteristics of bacterial skin infections in children compared to adults at a tertiary dermatologic center. | bacterial skin infections in children and adults are caused by different organisms with different antimicrobial susceptibility. | 1999 | 10487446 |
| regional excitatory and inhibitory amino acid concentrations in noda epileptic rat (ner) brain. | we measured regional concentrations of excitatory and inhibitory amino acids in noda epileptic rat (ner) brains to investigate the mechanisms responsible for spontaneous generalized seizures. compared with fisher 344 (f344) rats and f1 hybrid (female ner x male f344 rat) rats, ners had significantly higher concentrations of glycine in the cerebellum. ners and f1 hybrid rats had significantly lower concentrations of taurine in the cerebellum than did f344 rats. although our findings do not explai ... | 1999 | 10487471 |
| ambient ozone concentrations induce airway hyperresponsiveness in some rat strains. | ozone is known to induce airway hyperresponsiveness (ahr) in humans and animals. previous studies in animals used high exposure levels and reported inconsistent results. the aim of this study was to investigate the effect of a single low-level ozone exposure on different inbred rat strains. nine rat strains were exposed to 0.05 parts per million (ppm) for 4 h and airway responsiveness to intravenous 5-hydroxytryptamine (ht) examined. bronchoalveolar lavage fluid (balf) was examined for the prese ... | 1999 | 10489839 |
| monoclonal antibodies raised against guillain-barré syndrome-associated campylobacter jejuni lipopolysaccharides react with neuronal gangliosides and paralyze muscle-nerve preparations. | guillain-barré syndrome and its variant, miller-fisher syndrome, are acute, postinfectious, autoimmune neuropathies that frequently follow campylobacter jejuni enteritis. the pathogenesis is believed to involve molecular mimicry between sialylated epitopes on c. jejuni lpss and neural gangliosides. more than 90% of miller-fisher syndrome cases have serum anti-gq1b and anti-gt1a ganglioside antibodies that may also react with other disialylated gangliosides including gd3 and gd1b. structural stud ... | 1999 | 10491405 |
| effects of a thirteen-week inhalation exposure to ethyl tertiary butyl ether on fischer-344 rats and cd-1 mice. | the 1990 clean air act amendments require that oxygenates be added to automotive fuels to reduce emissions of carbon monoxide and hydrocarbons. one potential oxygenate is the aliphatic ether ethyl tertiary butyl ether (etbe). our objective was to provide data on the potential toxic effects of etbe. male and female fisher 344 rats and cd-1 mice were exposed to 0 (control), 500, 1750, or 5000 ppm of etbe for 6 h/day and 5 days/wk over a 13-week period. etbe exposure had no effect on mortality and ... | 1999 | 10496682 |
| relationship between the conserved alpha subunit arginine 107 and effects of phosphate on the activity and stability of vibrio harveyi luciferase. | the arg107 of the alpha subunit is a conserved residue for all known bacterial luciferases. the phosphate moiety of the reduced flavin mononucleotide (fmnh(2)) side chain has been hypothesized to be anchored at this site (a. j. fisher, f. m. raushel, t. o. baldwin, and i. rayment biochemistry 34, 6581-6586, 1995). mutations of alphaarg107 of the vibrio harveyi luciferase to alanine, serine, and glutamate were carried out to test such a hypothesis. these variants were characterized and compared w ... | 1999 | 10496975 |
| human anti-pseudomonas aeruginosa outer membrane proteins igg cross-protective against infection with heterologous immunotype strains of p. aeruginosa. | in order to develop an effective means to treat and prevent pseudomonas aeruginosa infections, we have purified p. aeruginosa outer membrane protein (oprs)-specific human igg antibody using a large-scale affinity column. in this study, we investigated the cross-protective activity of the purified anti-oprs igg against various immunotype strains of p. aeruginosa. the anti-oprs igg reacted with oprs isolated from seven fisher-devlin immunotype strains of p. aeruginosa and was able to promote opson ... | 1999 | 10497864 |
| pathogenesis of guillain-barré and miller fisher syndromes subsequent to campylobacter jejuni enteritis. | some patients developed guillain-barré syndrome (gbs) after the administration of bovine gangliosides. patients with gbs subsequent to campylobacter jejuni enteritis frequently have igg antibody to gm1 ganglioside. miller fisher syndrome (mfs), a variant of gbs, is associated with igg antibody to gq1b ganglioside. we showed the existence of molecular mimicry between gm1 and lipopolysaccharide of c. jejuni isolated from patients with gbs, and that between gq1b and c. jejuni lipopolysaccharides fr ... | 1999 | 10507987 |
| [campylobacter jejuni enteritis and guillain-barré syndrome]. | some patients developed guillain-barré syndrome after the administration of bovine brain ganglioside. patients with guillain-barré syndrome subsequent to campylobacter jejuni enteritis frequently have igg antibody to gm1 ganglioside. miller fisher syndrome, a variant of guillain-barré syndrome, is associated with igg antibody to gq1b ganglioside. my colleagues and i showed the existence of molecular mimicry between gm1 and lipopolysaccharide of c. jejuni isolated from a patient with guillain-bar ... | 1999 | 10511801 |
| [a case of subarachnoid hemorrhage verified as cerebral vasospasm by using three-dimensional ct angiography (3 d-cta): reference to comparison with conventional angiography]. | the authors present a case of aneurysmal subarachnoid hemorrhage that were verified as cerebral vasospasm by using both three-dimensional ct angioraphy (3 d-cta) and conventional angiography. a 45-year-old man was referred to our department 4th day after sudden onset of a severe headache. on admission, emergency 3 d-cta showed the cerebral vasospasm involving m 1 segment. conventional angiography performed at the same day of the left internal carotid artery confirmed the cerebral vasospasm of th ... | 1999 | 10511960 |
| hippocampal lesions prevent trace eyeblink conditioning in the freely moving rat. | the effect of hippocampal aspiration lesions on trace eyeblink conditioning was examined in young, freely-moving f1 hybrid rats (fisher 344 x brown norway). rats which received either bilateral neocortical or bilateral hippocampal aspiration lesions were compared with each other or with sham lesioned control rats. the rats were trained with a 250 ms tone conditioning stimulus (cs), a 250 ms stimulus free trace interval and a 100 ms corneal airpuff unconditioned stimulus (us). rats with lesions o ... | 1999 | 10512579 |
| prevention of chronic renal allograft rejection in rats with an oral endothelin a receptor antagonist. | chronic rejection is the most common cause of graft loss in renal transplantation. the pathomechanisms underlying chronic rejection are poorly understood, and no treatment has yet successfully been established. we hypothesized that, in analogy to models of reduced renal mass, the administration of a selective endothelin (et) a receptor antagonist could improve the course of chronic rejection in renal allografts. | 1999 | 10515373 |
| use of a skin explant model for predicting gvhd in hla-matched bone marrow transplants - effect of gvhd prophylaxis. | over the last decade we have successfully evaluated the use of a human skin explant assay for predicting acute gvhd in hla-matched sibling transplants. in the present study, we modified gvhd prophylaxis on an individual patient basis depending on the gvhd outcome predicted by the skin explant model. we have summarised our previous data describing how the skin explant assay results correctly predict gvhd occurrence and severity in 45/56 patients (80%); p< 0.0001, chi2 19.97, df = 1. in a further ... | 1999 | 10516697 |
| [etiologic, clinical and socio-democratic characteristics of acute diarrhea in venezuela]. | in four cities of venezuela a study was carried out to evaluate the epidemiological, clinical, and etiological characteristics of acute diarrhea in children under 5 years of age. the study was done between june 1993 and may 1995 and involved children who were seen in a hospital, 2,552 with diarrhea and 793 controls. the fisher exact test was used for the statistical analysis of the results. rotaviruses were the most important agents, both in terms of their frequency (30%) and their association w ... | 1999 | 10517091 |
| [measurement of antiganglioside autoantibodies by immunodot-blot assay: clinical importance in peripheral neuropathies]. | we retrospectively evaluated measurement data and clinical relevance of autoantibodies to gangliosides in peripheral neuropathies (pn). the igg and igm antiganglioside autoantibodies were determined by our own immunodot-blot assay on membrane and by enzyme-linked immunosorbent assay (elisa) in sera of 1,342 patients with peripheral neuropathies. anti-gm1 and anti-gd1b autoantibodies formed a part of the normal autoantibody repertoire and were common place in 12% of normal subjects and in 14% of ... | 1999 | 10518060 |
| periarticular tissue levels of cytokine- and endothelin-1-like immunoreactivity during the course of antigen-induced arthritis in the rat. | to correlate the changes in periarticular tissue levels of pro-inflammatory cytokines and endothelin-1 (et-1) to local pathophysiology in rats with antigen-induced arthritis (aia). | 1999 | 10522804 |
| salivary carbonic anhydrase isoenzyme vi. | the carbonic anhydrases (cas) participate in the maintenance of ph homeostasis in various tissues and biological fluids of the human body by catalysing the reversible reaction co2 + h2o hco3- + h+ (davenport & fisher, 1938; davenport, 1939; maren, 1967). carbonic anhydrase isoenzyme vi (ca vi) is the only secretory isoenzyme of the mammalian ca gene family. it is exclusively expressed in the serous acinar cells of the parotid and submandibular glands, from where it is secreted into the saliva. i ... | 1999 | 10523402 |
| role of potassium channels in the central neurogenic neuroprotection elicited by cerebellar stimulation in rat. | electrical stimulation of the cerebellar fastigial nucleus (fn) in spontaneously hypertensive (shr), wistar-kyoto (wky) and fisher rats reduced, by approximately 50%, the infarctions produced by occlusion of the middle cerebral artery. blockade of atp-dependent potassium (k-atp) channels with glibenclamide (i.c.v.) abolished salvage only in the shr rat. while blockade of k-atp channels failed to abolish salvage in wky and fisher rats, participation of potassium channels in neurogenic neuroprotec ... | 1999 | 10526150 |
| clinical presentation and outcome of guillain-barré and related syndromes in relation to anti-ganglioside antibodies. | we correlated the clinical features of 78 patients with guillain-barré syndrome (gbs) or related variants, with the presence of serum antibodies to the gangliosides gm1, gm2, gd1a, gd1b and gq1b in order to determine whether these antibodies may influence the clinical presentation or outcome of gbs. sixty-three patients had typical gbs (81%), nine a pure motor form (11%), three a paraparetic form (4%), and three had miller fisher syndrome (mfs). igg or igm (or both) anti-ganglioside antibodies w ... | 1999 | 10526187 |
| serum antibody against a peripheral nerve myelin ganglioside, lm1, in guillain-barré syndrome. | serum igg antibody against lm1, the predominant ganglioside in the human peripheral nerve myelin, was found in 7 out of 140 patients with guillain-barré syndrome (gbs) in the acute phase, 1 out of 33 patients with chronic inflammatory demyelinating polyneuropathy (cidp), and 2 out of 47 patients with miller fisher syndrome (mfs). anti-lm1 igm antibody was detected only in 2 patients, each with gbs and mfs. the clinical and electrophysiological features of the seven gbs patients with anti-lm1 igg ... | 1999 | 10526188 |
| significance of endogenous heat shock protein in adjuvant arthritis. | to investigate the role of endogenous heat shock protein (hsp) (rat 60 kda hsp, rhsp 60) in adjuvant arthritis (aa), the expression of rhsp 60 in aa-susceptible lewis rats and aa-resistant fisher rats was studied. | 1999 | 10529142 |
| studies in thoracic aortic graft infections: the development of a porcine model and a comparison of collagen-impregnated dacron grafts and cryopreserved allografts. | a porcine model of thoracic aortic graft infection was created, and various anatomic sites and the timing of inoculation of the graft to induce infection were investigated. ultimately, the ability of cryopreserved allograft to resist infection was compared with that of collagen-impregnated dacron graft. | 1999 | 10534691 |
| cell-based gene transfer to the pulmonary vasculature: endothelial nitric oxide synthase overexpression inhibits monocrotaline-induced pulmonary hypertension. | to circumvent the problems of in vivo transfection and avoid the use of viral vectors or proteins, we sought to establish whether smooth-muscle cells (smcs) transfected ex vivo could be delivered via the systemic venous circulation into the pulmonary bed to achieve local transgene expression in the lung. primary cultures of pulmonary artery smcs from fisher 344 rats were labeled with a fluorescent, membrane-impermeable dye chloromethyl trimethyl rhodamine or transfected with the beta-galactosida ... | 1999 | 10536116 |
| potassium depletion: renal membrane lipid metabolism and na/h exchanger abundance in aged f344 x bnf1 rats. | potassium depletion (-k) is a common electrolyte abnormality in elderly humans, occurring after diuretic use or poor oral intake. we hypothesized that aging would result in an increase in renal membrane lipid metabolism in both control and -k, and that the na/h exchanger's protein abundance to -k would be blunted. young and senescent non-obese male fisher 344 x brown-norway f1 rats (f344 x bnf1) were fed either a normal or a k-deficient diet for 7 days. at 24-h, 32p incorporation was measured fo ... | 1999 | 10536641 |
| participation of the fas-signaling system in the initiation of germ cell apoptosis in young rat testes after exposure to mono-(2-ethylhexyl) phthalate. | the fas-signaling system is composed of the interacting proteins fas (cd95/apo-1) and fas ligand (fasl, cd95l, apo-1l) and is proposed to act in the testis as a paracrine signaling mechanism by which fasl-expressing sertoli cells initiate apoptosis of fas-bearing germ cells. here we describe alterations in the expression of fas and fasl in the testis after the intimate physical association between sertoli cells and germ cells is disrupted by exposure to the sertoli cell toxicant mono-2-(ethylhex ... | 1999 | 10544061 |
| [cerebral lacunae: still under debate]. | during the last fifteen years, a new interest has been shown in cerebral lacunes due to the development of brain imagery using magnetic resonance imaging (mri). the concept of lacunar infarction as defined par fisher (lacunar hypothesis) can no longer be accepted. clinical data showed that the so-called lacunar clinical syndromes were far from being specific and could be observed in corticosubcortical infarcts. epidemiological data pointed out that hypertensive disease was not found in many case ... | 1999 | 10546297 |
| eighty three years of the guillain-barré syndrome: clinical and immunopathologic aspects, current and future treatments. | it is now 83 years that guillain, barré and strohl described the first two cases of an acute paralytic illness with the typical "dissociation albumino-cytologique" in the cerebrospinal fluid. since then, the full spectrum of gbs has been documented in hundreds of cases ranging from acute inflammatory demyelinating polyneuropathy to the pure motor variants and the miller fisher syndrome. during the last 10 years, detailed immunopathologic features have been described and new triggering and possib ... | 1999 | 10546299 |
| highly selective targeting of ovarian cancer with the photosensitizer peg-m-thpc in a rat model. | photodynamic therapy (pdt) uses light to activate a photosensitizer that has been absorbed or retained preferentially by cancer cells after systemic administration. the first pegylated photosensitizer, tetrakis-(m-methoxypolyethylene glycol) derivative of 7,8-dihydro-5,10,15,20-tetrakis(3-hydroxyphenyl)-21,23-[h]-porphyrin (peg-m-thpc), was evaluated to target selectively unresectable pelvic ovarian cancer bulks. our goals were two-fold: (1) to establish an ovarian cancer model suitable for the ... | 1999 | 10546556 |
| lack of cd44 variant 6 expression in advanced extrahepatic bile duct/ampullary carcinoma. | transmembrane proteins of the cd44 family play roles in cell-cell and cell-matrix interactions, and their aberrant expression has been reported to be associated with the growth and metastasis of various tumors. the authors examined cd44 standard (cd44st) and cd44 variant 6 (cd44v6) expression in extrahepatic bile duct (ehbd)/ampullary carcinoma. | 1999 | 10547541 |
| behavioral and neurochemical differences between fischer 344 and harlan-wistar rats raised identically. | inbred fisher 344 and outbred harlan-wistar rats were compared in the elevated plus maze, the black-and-white box, the social interaction test, and a modified open-field test, to assess the contribution of genetic factors to aversion-motivated behavior. all animals used were born and raised under identical conditions. compared to the wistar rats, the fischer rats displayed a more pronounced fearful behavior in all tests. in a separate microdialysis study, the relationship between behavioral vari ... | 1999 | 10547925 |
| after radiotherapy testosterone stimulation is unable to increase growth in the dunning r3327-pap prostate tumour. | a study was carried out to investigate whether testosterone treatment is able to influence tumour growth in a rat prostatic adenocarcinoma previously treated with castration and high-dose fractionated irradiation. copenhagen x fisher rats bearing the androgen-sensitive, well-differentiated dunning r3327-pap tumour were castrated and thereafter treated with external beam radiation with photons from a 4-mv linear accelerator. one month after irradiation, substitution with subcutaneous testosterone ... | 1999 | 10550524 |
| inactivation of the ink4a/arf locus frequently coexists with tp53 mutations in non-small cell lung cancer. | inactivation of the p16 (ink4a)/retinoblastoma (rb) or tp53 biochemical pathway is frequent event in most human cancers. recent evidence has shown that p14arf binds to mdm2 leading to an increased availability of wild type tp53 protein. functional studies also support a putative tumor suppressor gene function for p14arf suggesting that p14arf or p53 inactivation may be functionally equivalent in tumorigenesis. to study the relative contribution of each pathway in tumorigenesis, we analysed and c ... | 1999 | 10557071 |
| protective effects of garlic juice against embryotoxicity of methylmercuric chloride administered to pregnant fischer 344 rats. | in order to investigate the beneficial effects of 0.5 or 1.0 g/kg korean garlic juice against the embryotoxicity of 20 mg/kg methylmercury chloride (mmc, ch3hgcl), pregnant fisher 344 rats were simultaneously orally administered on day 7 of gestation. on day 20 of gestation the dams were laparotomized under ether anesthesia, and the fetuses were removed and examined for toxicity of methylmercury. garlic juice depressed the toxicity in terms of some parameters. in the case of simultaneous treatme ... | 1999 | 10565261 |
| dual-label studies with [125i]-3(r)/[131i]-3(s)-bmipp show similar metabolism in rat tissues. | biodistribution studies with the radioiodinated 3(r)- and 3(s)-isomers of 15-(p-iodophenyl)-3-methylpentadecanoic acid (bmipp) in rats have shown that 3(r)-bmipp has 20%-25% higher heart uptake than 3(s)-bmipp (15-180 min). in contrast, the 3(s)-isomer has slightly higher liver uptake, and uptake in other tissues examined is similar. | 1999 | 10565790 |
| detection of serum anticerebellar antibodies in patients with miller fisher syndrome. | we performed western blot analysis to detect anticerebellar antibodies in the serum of patients with miller fisher syndrome (mfs). we studied 7 mfs patients, 6 guillain-barré syndrome (gbs) patients and 10 normal healthy persons as controls. six mfs patients (86%) had igg antibodies against mouse cerebellar protein, whereas 3 gbs patients (50%) and 4 healthy controls (40%) had antibodies. the mean number of antibodies in the serum of patient with mfs was 2.43, which was significantly more than t ... | 1999 | 10567821 |
| nitric oxide synthase in aging rat skeletal muscle. | the neuronal isoform of nitric oxide synthase (nos) is expressed at high concentrations in skeletal muscle, and no influences muscle contractility, glucose utilization, and free radical damage or protection. nos activity and expression was evaluated in extensor digitorum longus (edl), soleus, and diaphragm of 8 and 24 month old fisher 344 rats. in 8-month-old animals, nos activity was highest in edl, which contained the highest percentage of nos containing fibers, and was lowest in soleus. nos a ... | 1999 | 10576333 |
| accumulation of dna damage in pre- and posthepatectomized liver of aged rats. | although the majority of the literature supports the concept that an accumulation of dna damage or a modification in the dna structure of postmitotic cells occurs with increasing age, there are also several reports that show no dna changes in these cells with increasing age. in the study reported here, two components of the dna damage hypothesis of aging were tested. young (4-6 months) and old (18-20 month) unirradiated or irradiated, pre- and posthepatectomized male fisher 344 rats were killed, ... | 1999 | 10580689 |
| evidence for double resistance to permethrin and malathion in head lice. | a rising prevalence of head lice among school children and rising sales of insecticides with anecdotal evidence of their treatment failure, led us to examine whether head lice in bristol and bath were resistant to the insecticides available for treating head lice. ten schools in bristol and bath were visited to collect field samples of head lice. a comparison was made of the survival rates of fully sensitive laboratory reared body lice and field samples of head lice on insecticide exposure. to c ... | 1999 | 10583056 |
| [the factors that influence the use of antibiotics in primary care]. | to identify factors influencing volume, quality and power of antibiotic prescription in primary care. | 1999 | 10590559 |
| the effects of carbon dioxide pneumoperitoneum on seeding of tumor in port sites in a rat model. | the use of laparoscopic surgical techniques for the resection of intraperitoneal malignancies has been rapidly increasing in recent years; concomitantly, tumor recurrences at trocar sites have also been reported. these reports bring into question the appropriateness of pneumoperitoneum and laparoscopic techniques for carcinoma removal. we hypothesized that the carbon dioxide pneumoperitoneum and instrumentation used during laparoscopic procedures contribute to a greater incidence of tumor implan ... | 1999 | 10601908 |
| liver regeneration in response to partial hepatectomy in rats treated with retrorsine: a kinetic study. | we have designed an experimental model in which transplantation of normal hepatocytes into rats previously treated with retrorsine (a naturally-occurring pyrrolizidine alkaloid) results in near-complete replacement of the recipient liver by donor-derived cells. two/thirds partial hepatectomy was found to be essential for this process to occur. to probe this finding, in the present study we describe the kinetics of liver regeneration in response to partial hepatectomy in rats given retrorsine. | 1999 | 10604581 |
| skeletal muscle tissue engineering using isolated myoblasts on synthetic biodegradable polymers: preliminary studies. | skeletal muscle is responsible for the control of voluntary movement and the maintenance of structural contours of the body. muscle loss or deficiency is encountered in various pathological states, and attempts to correct them have been employed with limited success. the aim of the present study was to tissue engineer three-dimensional vascularized skeletal muscle using isolated myoblasts attached to synthetic biodegradable polymer for tissue replacement in the enhancement of muscle regeneration ... | 1999 | 10611544 |
| x-ray structures of the dictyostelium discoideum myosin motor domain with six non-nucleotide analogs. | the three-dimensional structures of the truncated myosin head from dictyostelium discoideum myosin ii complexed with dinitrophenylaminoethyl-, dinitrophenylaminopropyl-, o-nitrophenylaminoethyl-, m-nitrophenylaminoethyl-, p-nitrophenylaminoethyl-, and o-nitrophenyl-n-methyl-aminoethyl-diphosphate.beryllium fluoride have been determined to better than 2.3-a resolution. the structure of the protein and nucleotide binding pocket in these complexes is very similar to that of s1dc.adp.bef(x) (fisher, ... | 2000 | 10617631 |
| enhanced mortality from perfluorocarbon administration in a rat model of kerosene aspiration. | aspiration of low-viscosity hydrocarbons may lead to fulminant pneumonitis and acute respiratory distress syndrome. animal and human studies suggest that partial liquid ventilation with perfluorocarbon improves gas exchange and pulmonary function in acute respiratory failure. the objective of this investigation was to determine the effect of intratracheal perfluorocarbon administration and a brief period of partial liquid ventilation on survival in a rat model of severe hydrocarbon aspiration. | 1999 | 10630269 |
| differential induction of hepatic drug-metabolizing enzymes by fenvaleric acid in male rats. | racemic fenvaleric acid [2-(4-chlorophenyl)-3-methyl-butanoic acid], the principal metabolite of fenvalerate, was administrated orally at 0.75, 1.5, and 3.0 mmol/kg body weight/day to fisher-344 male rats for 7 days. both pure enantiomers of fenvaleric acid were administered at 1.5 mmol/kg body weight/day; the clofibric acid at the same concentration was used as a positive control. hepatic enzyme activities were measured. results obtained clearly show that fenvaleric acid induced numerous hepati ... | 1999 | 10630566 |
| serum positive botulism with neuropathic features. | a 32-year-old man presented with multiple cranial neuropathies and his serum was positive for botulism type b. however, serial electrodiagnostic studies were consistent with a primarily neuropathic process, such as fisher syndrome, rather than a neuromuscular junction disorder. electrodiagnostic study findings in patients with presumed neuromuscular junction disorders may mimic findings suggestive of a neuropathic process, or the bioassay for botulism may be falsely positive in patients with fis ... | 2000 | 10638887 |
| overexpression of vascular endothelial growth factor164 and its co-receptor neuropilin-1 in estrogen-induced rat pituitary tumors and gh3 rat pituitary tumor cells. | we have shown previously that the vegf system plays a crucial role in regulation of tumor angiogenesis during the development of estrogen-induced prolactin-secreting pituitary tumors in fisher 344 rats. studies also suggested that both endothelial and non-endothelial cells expressed vegf. however, several questions concerning the vegf signals in regulation of estrogen-induced angiogenesis in rat pituitary remained unanswered. vegf exists in a number of isoforms in human and rodent tissue (i.e., ... | 2000 | 10639567 |
| angiogenesis inhibitor tnp-470 suppresses the progression of experimentally-induced hepatocellular carcinoma in rats. | we evaluated the effects of angiogenesis inhibitor, tnp-470, on hepatocellular carcinoma (hccs) induced by a choline-deficient l-amino acid defined (cdaa) diet in rats. male fisher 344 rats were fed cdaa for 68 weeks. rats were treated by subcutaneous injection of tnp-470 (15 mg/kg) or saline (control) three times per week from 53 to 68 weeks. at the end of the experiment, we determined the frequency and size of hccs and glutathione s-transferase placental form (gstp)-positive lesions, histology ... | 2000 | 10639583 |
| correlated cortical populations can enhance sound localization performance. | neurons within cortical populations often evidence some degree of response correlation. correlation has generally been regarded as detrimental to the decoding performance of a theoretical vector-averaging observer making inferences about the physical world-for example, an observer estimating the location of a sound source. however, if an alternative decoder is considered, in this case a maximum likelihood estimator, performance can improve when responses in the population are correlated. improve ... | 2000 | 10641650 |
| is it wise to restrict fat in the diets of children? | the proponents of fat-restricted diets for children argue that low-fat diets given in childhood will prevent the development of atherosclerosis in adulthood, low-fat diets given a childhood will condition children to prefer low-fat diets in adulthood, and low-fat diets for children are safe. there is no evidence that low-fat diets in childhood will prevent atherosclerosis in adulthood. in fact, studies of migrating populations indicate that immigrants to the united states from third world countr ... | 2000 | 10645998 |
| fas ligand expression in esophageal carcinomas and their lymph node metastases. | although esophageal adenocarcinomas (eadca) have been shown to have substantially reduced or absent fas expression, the status of fas ligand (fasl) in these tumors, especially adenocarcinomas, is largely unknown. | 2000 | 10649242 |
| the effects of a specific alpha(2)-adrenoceptor antagonist, atipamezole, on cognitive performance and brain neurochemistry in aged fisher 344 rats. | the present experiments investigated the effects of a specific and potent alpha(2)-adrenoceptor antagonist, atipamezole, on cognitive performance and neurochemistry in aged rats. aged control fisher 344 rats, which had lower activities of choline acetyltransferase in the frontal cortex, were impaired in the acquisition of the linear arm maze task both in terms of repetition errors and their behavioural activity (the speed of arm visits), and they needed longer time to complete this task as compa ... | 2000 | 10650154 |
| quantitative analysis of three-dimensional complexity and connectivity changes in trabecular microarchitecture in relation to aging, menopause, and inflammation. | there are several types of bone loss besides that associated with normal aging, eg, that associated with the menopause, and that associated with chronic inflammation, and these are considered to be caused by different mechanisms. the microarchitecture that results from these different bone-loss mechanisms would not be the same. the purpose of this study was to investigate differences in the three-dimensional trabecular microarchitecture in various types of osteopenia, using microcomputed tomogra ... | 1999 | 10664426 |
| a double-blind, placebo-controlled trial of testosterone therapy for hiv-positive men with hypogonadal symptoms. | the goal was to evaluate the efficacy of testosterone in alleviation of hypogonadal symptoms (diminished libido, depressed mood, low energy, and depleted muscle mass) in men with symptomatic human immunodeficiency virus illness. | 2000 | 10665616 |
| genetic and biochemical evidence of a campylobacter jejuni capsular polysaccharide that accounts for penner serotype specificity. | campylobacter jejuni, a gram-negative spiral bacterium, is the most common bacterial cause of acute human gastroenteritis and is increasingly recognized for its association with the serious post-infection neurological complications of the miller-fisher and guillain-barré syndromes. c. jejuni lipopolysaccharide (lps) is thought to be involved in the pathogenesis of both uncomplicated infection and more serious sequelae, yet the lps remains poorly characterized. current studies on c. jejuni sugges ... | 2000 | 10672176 |
| toluene diisocyanate enhances substance p in sensory neurons innervating the nasal mucosa. | inhalation of irritants, such as toluene diisocyanate (tdi), stimulates substance p (sp) release from peripheral processes of sensory neurons innervating the airways. the purpose of this study was to determine if tdi inhalation affects intraneuronal levels of sp and preprotachykinin (ppt) messenger rna (mrna) in the sensory neurons of the trigeminal ganglion (tg) which innervate the nasal epithelium. the nasal cavity of fisher-344 rats was instilled with rhodamine-labeled latex microspheres. ten ... | 2000 | 10673198 |
| striatal trophic activity is reduced in the aged rat brain. | our previous studies demonstrated that the survival of a mesencephalic graft was reduced in aged animals suggesting an age-related decline in target-derived neurotrophic activity. we tested this hypothesis by examining dopamine (da) and trophic activities from the striatum of intact or unilateral 6-hydroxydopamine (6-ohda) lesioned rats of increasing age. fisher 344 rats were 4, 12, 18, and 23 months old (m.o.) at sacrifice. half the animals had received unilateral 6-ohda lesions of the mesostri ... | 2000 | 10677639 |
| regulation of fgf soluble receptor type 1 (sr1) expression and distribution in developing, degenerating, and fgf2-treated retina. | the spatial and temporal patterns of expression and content of the fibroblast growth factor (fgf) soluble receptor sr1, a specific inhibitor of fgf, were investigated during embryonic and postnatal development of the retina in fisher rats. as early as at embryonic day 18 (e18), sr1 mrna and protein were detected in the retina. sr1 protein was strongly associated with the differentiating ganglion cells and its distribution paralleled the radial pattern of retinal development, from center to perip ... | 2000 | 10679927 |
| [a preliminary study on the association between hla-dpb1 gene and pathological myopia]. | to investigate the association between distribution of human leucocyte antigen class ii dpb1 alleles and pathological myopia (pm) among chinese. | 1997 | 10680551 |
| group b streptococcus: to culture or not to culture? | to determine if universal group b streptococcus (gbs) culturing and antibiotic prophylaxis of obstetric patients decreased the incidence of neonatal early-onset gbs sepsis and mortality and maternal chorioamnionitis. | 1999 | 10685253 |
| laboratory enamel solubility reduction and fluoride uptake from enamelon dentifrice. | the presence of calcium-releasing ingredients in toothpastes containing sodium fluoride is usually avoided to prevent loss of active fluoride due to the formation of the insoluble calcium salt. the purpose of this study was to determine the bio-availability of fluoride from enamelon toothpaste (e), which simultaneously supplies fluoride, calcium and phosphate salts from a dual dispensing package. fluoride uptake into artificially lesioned enamel cores due to the use of the test dentifrice (e) di ... | 1999 | 10686852 |
| is geomagnetic activity a risk factor for sudden unexplained death in epilepsies? | to test the hypothesis that sudden unexplained death (sud) in epilepsy is related to geomagnetic activity. | 2000 | 10690984 |
| apoptosis during goitre involution - the role of bcl-2. | goitrogenesis is accompanied by hyperplasia and hypertrophy and involves tissue remodelling and angiogenesis. during the involution of the goitre there must be removal of this increased thyroid volume, in addition to further remodelling, which may involve apoptosis. we investigated apoptosis in the involuting rat thyroid using male fisher rats that were on a goitrogenic regimen for 14 days and then returned to a normal diet. thyroid weights increased fourfold with the goitrogenic regimen. during ... | 2000 | 10694372 |
| the immunopathogenesis of miller fisher syndrome. | over the past decade, remarkable progress has been made in our understanding of the pathogenesis of miller fisher syndrome (mfs), a clinical variant of guillain barré syndrome (gbs). mfs comprises the clinical triad of ataxia, areflexia and ophthalmoplegia. it is associated with acute-phase igg antibodies to gq1b and gt1a gangliosides in over 90% of cases which are highly disease specific. like gbs, mfs is a post-infectious syndrome following diverse infections, but particular attention has been ... | 1999 | 10695710 |
| pathogenesis of guillain-barré syndrome. | recent neurophysiological and pathological studies have led to a reclassification of the diseases that underlie guillain-barré syndrome (gbs) into acute inflammatory demyelinating polyradiculoneuropathy (aidp), acute motor and sensory axonal neuropathy (amsan) and acute motor axonal neuropathy (aman). the fisher syndrome of ophthalmoplegia, ataxia and areflexia is the most striking of several related conditions. significant antecedent events include campylobacter jejuni (4-66%), cytomegalovirus ... | 1999 | 10695718 |
| xenografting human t2 sympathetic ganglion from hyperhidrotic patients provides short-term restoration of catecholaminergic functions in hemiparkinsonian athymic rats. | previous studies have suggested that allografting peripheral sympathetic ganglia, such as superior cervical ganglia, partially relieves clinical or behavioral deficits in parkinsonian patients and animals. however, removal of these ganglia can cause homer's syndrome, which limits the utilization of this approach. hyperhidrosis, a disease of excessive sweating, is commonly seen in young orientals. treatment of hyperhidrosis often involves surgical removal of the second thoracic sympathetic gangli ... | 1999 | 10701487 |
| role of megalin (gp330) in transcytosis of thyroglobulin by thyroid cells. a novel function in the control of thyroid hormone release. | when thyroglobulin (tg) is endocytosed by thyrocytes and transported to lysosomes, thyroid hormones (t4 and t3) are released. however, some internalized tg is transcytosed intact into the bloodstream, thereby avoiding proteolytic cleavage. here we show that megalin (gp330), a tg receptor on thyroid cells, plays a role in tg transcytosis. following incubation with exogenous rat tg at 37 degrees c, fisher rat thyroid (frtl-5) cells, a differentiated thyroid cell line, released t3 into the medium. ... | 2000 | 10702280 |
| age and insulin-like growth factor-1 modulate n-methyl-d-aspartate receptor subtype expression in rats. | n-methyl-d-aspartate (nmda) receptors have been reported to have an important role in synaptic plasticity and neurodegeneration. two major subtypes of these receptors, nmdar1 and nmdar2, are present in brain and heterogeneity of these receptors have been reported to define specific functional responses. in this study, the effects of age and chronic insulin-like growth factor-1 (igf-1) administration on nmda receptor density and subtype expression were investigated in frontal cortex, ca1, ca2/3 a ... | 2000 | 10704784 |
| sdz-rad prevents manifestation of chronic rejection in rat renal allografts. | chronic rejection remains the most frequent cause of renal graft loss over the long term. however, effective treatment of this process is not yet available. sdz-rad (40-o-[2-hydroxyethyl]-rapamycin) is a new, orally active rapamycin derivative with potent immunosuppressive activity. we have examined the effects of sdz-rad in a well-established model of chronic renal allograft rejection in rats. | 2000 | 10708101 |
| possible involvement of proteasome inhibition in aging: implications for oxidative stress. | oxidative stress may contribute to the cellular alterations, which occur as the result of aging, and the nervous system is particularly vulnerable to aging associated oxidative injury. the multicatalytic proteasome (mcp) is responsible for the majority of protein degradation and is sensitive to oxidative stress. to determine if mcp activity is altered during aging, studies were conducted in multiple tissues from aged fisher 344 rats. analysis of heart, lung, kidney, and liver revealed decreased ... | 2000 | 10708250 |
| subarachnoid hemolysate produces dna fragmentation in a pattern similar to apoptosis in mouse brain. | stroke and traumatic brain/spinal cord injuries are often associated with hemorrhage. despite the relative frequency of hemorrhage in the central nervous system (cns), little is known about what role blood and hemoglobin (hb) play in mediating cellular injury. since hb and hemolysate have been associated with generation of oxidative stress and cell injury, we examined whether apoptosis was present after cortical exposure to subarachnoid hemolysate. subarachnoid hemorrhage (sah) was induced in cd ... | 2000 | 10708682 |