| electron microscopic assessment of cervical punch biopsies in women followed-up for human papillomavirus (hpv) lesions. | cervical punch biopsies from 102 women prospectively followed-up for hpv (human papillomavirus) lesions (flat, inverted and papillomatous condylomas) were assessed on transmission electron microscopy (tem) for the presence of hpv particles. the virus discovery rate was correlated with the other characteristics of significance to the clinical behaviour (natural history) of these lesions, i.e. the colposcopic pattern, expression of papillomavirus genus specific structural antigens (demonstrated by ... | 1985 | 2988479 |
| pulsed electromagnetic fields promote collagen production in bone marrow fibroblasts via athermal mechanisms. | primary and passaged cultures of fibroblasts (rbmfs) raised from the bone marrow stroma of young rabbits were treated with pulsed electromagnetic fields (pemfs) from the start of each culture until 1 week after they became confluent. the pemf treatment had no effect on cell proliferation, estimated by phase contrast microscopy, by 3h-thymidine incorporation into dna, or by total dna assay. collagen production, estimated by conversion of 3h-proline to 3h-hydroxyproline in nondialyzable material w ... | 1985 | 2988718 |
| specific binding of radioiodinated granulocyte-macrophage colony-stimulating factor to hemopoietic cells. | the hemopoietic growth factor granulocyte-macrophage colony-stimulating factor, gm-csf, specifically controls the production of granulocytes and macrophages. this report describes the binding of biologically-active 125i-labeled murine gm-csf to a range of hemopoietic cells. specific binding was restricted to murine cells and neither rat nor human bone marrow cells appeared to have surface receptors for 125i-labeled gm-csf. 125i-labeled gm-csf only appeared to bind specifically to cells in the my ... | 1985 | 2990915 |
| adrenergic receptors: possible mechanism of inverse regulation of alpha- and beta-receptors. | many physiologic and pathologic conditions, including bronchial asthma, are associated with inverse changes in alpha- and beta-receptor-mediated responses in various tissues. the direction of the change elicited by a given stimulus is tissue specific, as exemplified by the actions of thyroid hormones: in the rat heart, hypothyroidism reduces beta- and increases alpha-receptor responses, whereas in the rat liver it has the opposite effects. a similar increase in beta- and decrease in alpha-recept ... | 1985 | 2991359 |
| the ultrastructural basis for synaptic transmission between primary muscle afferents and neurons in clarke's column of the cat. | the synaptic connection between primary muscle afferents and dorsal spinocerebellar tract (dsct) neurons has been studied in an attempt to reveal some of the mechanisms underlying excitatory transmission in the mammalian central nervous system. previous electrophysiological experiments have shown that the excitatory postsynaptic potentials (epsps) evoked dsct neurons by impulses in a single muscle afferent fluctuate in amplitude. these fluctuations occur between discrete amplitudes which are sep ... | 1985 | 2991483 |
| the ultrastructural basis for synaptic transmission between primary muscle afferents and neurons in clarke's column of the cat. | the synaptic connection between primary muscle afferents and dorsal spinocerebellar tract (dsct) neurons has been studied in an attempt to reveal some of the mechanisms underlying excitatory transmission in the mammalian central nervous system. previous electrophysiological experiments have shown that the excitatory postsynaptic potentials (epsps) evoked dsct neurons by impulses in a single muscle afferent fluctuate in amplitude. these fluctuations occur between discrete amplitudes which are sep ... | 1985 | 2991483 |
| diverse involvements of ni protein in superoxide anion production in polymorphonuclear leukocytes depending on the type of membrane stimulants. | effects of islet-activating protein (iap) were examined to assess the involvement of the guanine nucleotide-binding regulatory protein responsible for inhibition of adenylate cyclase system (ni protein) in the superoxide anion (o-2) production in polymorphonuclear leukocytes (pmnl) stimulated with various agents. n-formyl-methionyl-leucyl-phenylalanine (fmlp)-stimulated o-2 production was inhibited by the pretreatment with iap. o-2 production induced by each of phorbol myristate acetate, concana ... | 1985 | 2992509 |
| rates of cholesterol synthesis and low-density lipoprotein uptake in the adrenal glands of the rat, hamster and rabbit in vivo. | the absolute rate of cholesterol acquisition from de novo synthesis and from receptor-dependent and receptor-independent low-density lipoprotein (ldl) uptake was determined in the adrenal glands of the rat, hamster and rabbit under in vivo conditions. the rate of incorporation of [3h]water into cholesterol in the adrenal gland was much higher in the hamster (1727 nmol/h per g) and rabbit (853 nmol/h per g) than in the rat (71 nmol/h per g). assuming that 23 atoms of 3h are incorporated into the ... | 1985 | 2992599 |
| delay in platelet recovery after bone marrow transplantation: impact of cytomegalovirus infection. | the effect of cytomegalovirus (cmv) infection on hematopoietic recovery after marrow-ablative chemoradiotherapy followed by autologous bone marrow transplantation (bmt) was studied in patients with non-hodgkin's lymphoma of high-grade malignancy and in patients with acute leukemia. the recovery of platelets after autologous bmt occurred significantly quicker in cmv-negative patients than in cmv-positive patients (platelets greater than 50,000 per cubic millimeter after 21 1/2 v 40 days, respecti ... | 1985 | 2994781 |
| results of investigations of the cell surface membranes composition of cells transformed by td-mutant of rsv. | | 1985 | 2995158 |
| insertion of dna sequences into the human chromosomal beta-globin locus by homologous recombination. | a 'rescuable' plasmid containing globin gene sequences allowing recombination with homologous chromosomal sequences has enabled us to produce, score and clone mammalian cells with the plasmid integrated into the human beta-globin locus. the planned modification was achieved in about one per thousand transformed cells whether or not the target gene was expressed. | 1985 | 2995814 |
| isolation and characterization of the human pulmonary surfactant apoprotein gene. | pulmonary surfactant is a phospholipid-protein complex which serves to lower the surface tension at the air-liquid interface in the alveoli of the mammalian lung and is essential for normal respiration. inadequate levels of surfactant at birth, a frequent situation in premature infants, results in respiratory failure. in all species examined, surfactant is composed primarily of dipalmitoylphosphatidylcholine and two major protein species of relative molecular mass (mr) 32,000 (32k) and 10k (refs ... | 1985 | 2995821 |
| calcitonin receptors in the rat mesencephalon mediate its analgesic actions: autoradiographic and behavioral analyses. | autoradiographic analyses of salmon calcitonin (sct) binding in the rat mesencephalon revealed an exceptionally high concentration of receptors in the ventral and ventrolateral segments of the periaqueductal gray matter (pag) extending along the entire rostral-caudal axis. relatively heavy labeling was also seen along a band extending ventrolaterally through the mesencephalic reticular formation. other receptor-rich areas include the nucleus linearis, pars compacta and lateralis of the substanti ... | 1985 | 2996694 |
| mutations affecting gyrase in haemophilus influenzae. | mutants separately resistant to novobiocin, coumermycin, nalidixic acid, and oxolinic acid contained gyrase activity as measured in vitro that was resistant to the antibiotics, indicating that the mutations represented structural alterations of the enzyme. one novr mutant contained an altered b subunit of the enzyme, as judged by the ability of a plasmid, pnov1, containing the mutation to complement a temperature-sensitive gyrase b mutation in escherichia coli and to cause novobiocin resistance ... | 1985 | 2997115 |
| isolation and characterization of ca2+-blind mutants of yersinia pestis. | the plasmid pcd1 is required for expression of the low-calcium response (lcr), virulence, and production of v antigen in yersinia pestis kim. five independent mutants constitutive for the lcr at 37 degrees c (lcrc) were obtained through ethyl methanesulfonate mutagenesis followed by ampicillin enrichment. a sixth, spontaneous mutant was obtained directly through ampicillin enrichment. these mutants failed to grow at 37 degrees c regardless of calcium concentration and produced v antigen constitu ... | 1985 | 2997127 |
| rflp for the human apolipoprotein b gene: ii;ecori. | | 1985 | 2997725 |
| [effect of tunicamycin on human cytomegalovirus replication]. | | 1985 | 2998078 |
| [effect of levamisole on the course of chronic cytomegalovirus infection in women]. | the therapeutic effect of orally given levamisole in the treatment of women with chronic cytomegalovirus infection was studied. the patients were examined serologically, cytologically, virologically; the factors of cell-mediated immunity were also studied. oral therapy with levamisole resulted in a definite increase of the cell-mediated immunity with a trend for decrease in antibody titres and reduced virus excretion. evaluation of the clinical effect of levamisole therapy, however, revealed no ... | 1985 | 2998093 |
| evaluation of a photolabile derivative of 1,25-dihydroxyvitamin d3 as a photoaffinity probe for 1,25-dihydroxyvitamin-d3 receptor in chick intestinal cytosol. | we evaluated the viability of 1 alpha, 25-dihydroxyvitamin d3-3 beta-[n-(4-azido-2-nitrophenyl)glycinate] (1,25-(oh)2-d3-ang), an analog of 1 alpha, 25-dihydroxyvitamin d3 (1,25-(oh)2-d3) as a photoaffinity probe for 1,25-(oh)2-d3 receptor in chick intestinal cytosol. a competitive-binding assay revealed that chick intestinal cytosolic 1,25-(oh)2- d3 receptor bound to 1,25-(oh)2-d3-ang approximately 20-times less effectively than it did to 1,25-(oh)2-d3. irradiation of 1,25-(oh)2-d3- ang in the ... | 1985 | 2998355 |
| autoxidation reactions of hemoglobin a free from other red cell components: a minimal mechanism. | rates of autoxidation reactions are determined for normal human hemoglobin a preparations which are extensively purified to remove all other redox active red cell components. the effects of superoxide dismutase, catalase, and hydroxyl radical scavengers on the reaction provide evidence for superoxide formation as the rate determing step followed by fast reactions that involve peroxide and hydroxyl radical. these results support a minimum overall mechanism for heme iron(ii) oxidation and dioxygen ... | 1985 | 2998382 |
| a monoclonal antibody raised to corpus callosum extract reacts with 2',3'-cyclic nucleotide 3'-phosphohydrolase. | monoclonal antibody against 2',3'-cyclic nucleotide 3'-phosphohydrolase (cnp) was generated by fusing mouse myeloma cells with spleen cells from balb/c mice immunized with delipidated white matter from rat corpus callosum. the antibody was characterized by solid-phase radioimmunoassay, immunoblot of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), immunoprecipitation from c6 glioma cells, and indirect immunofluorescence staining of monolayer cultures containing oligodendrocy ... | 1986 | 2999339 |
| pyrimidinones. 1. 2-amino-5-halo-6-aryl-4(3h)-pyrimidinones. interferon-inducing antiviral agents. | interferon induction and antiviral activity was discovered with 2-amino-5-bromo-6-phenyl-4(3h)-pyrimidinone. an analogue study incorporating a series of 2-amino-5-substituted-6-arylpyrimidinones revealed that the most potent interferon inducers were mono- and difluorophenyl analogues. these same analogues were also potent antiviral agents against semliki forest virus and herpes simplex type 1. in addition the monomethoxyphenyl analogues were potent antiviral agents but weak interferon inducers. ... | 1985 | 2999405 |
| collagen accumulation can continue with decreased prolyl hydroxylase activity in the liver. | a single dose of dimethylnitrosamine dose-relatedly increased total hepatic hydroxyproline content in rats 14 days after the dosing. in cases of 35 mg/kg of dimethylnitrosamine, it increased rapidly to 1.7 times the normal level within 14 days. this increase persisted thereafter until 84 days. hepatic collagen prolyl hydroxylase activity was 1.8 times the normal level by the fourth day after the dosing but normalized within 14 days. it decreased further to levels significantly lower than those i ... | 1985 | 2999546 |
| the effects of castration and/or methimazole feeding on the pituitary response to temperature extremes by cockerels. | the pituitary of goitrogen-treated white leghorn cockerels is smaller in size than control birds and the pituitaries of castrated cockerels is nearly twice the size of control birds. the pituitary cells generated by these treatments may not be functional thyrotrophs or gonadotrophs and may not be able to respond to their usual stimuli. low ambient temperature is a well-known stimulus to the thyroid gland acting through pituitary tsh. cyclic-amp-dependent protein kinase activity levels are used h ... | 1985 | 3000864 |
| the effects of castration and/or methimazole feeding on the pituitary response to temperature extremes by cockerels. | the pituitary of goitrogen-treated white leghorn cockerels is smaller in size than control birds and the pituitaries of castrated cockerels is nearly twice the size of control birds. the pituitary cells generated by these treatments may not be functional thyrotrophs or gonadotrophs and may not be able to respond to their usual stimuli. low ambient temperature is a well-known stimulus to the thyroid gland acting through pituitary tsh. cyclic-amp-dependent protein kinase activity levels are used h ... | 1985 | 3000864 |
| repression of adenovirus early gene expression by coinfection with a temperature-sensitive mutant in the immediate-early gene of pseudorabies virus. | wild-type adenovirus was coinfected with a mutant temperature sensitive for the immediate-early gene of pseudorabies virus. at the nonpermissive temperature, this mutant, tsg, strongly inhibited the transcription of all adenovirus early genes, including e1a. this inhibition was not observed with wild-type pseudorabies virus coinfection or with tsg coinfection at the permissive temperature. the level of repression was dependent upon the ratio of tsg to adenovirus in the infection. the results sug ... | 1986 | 3001339 |
| relationship of acth1-39-immunostained fibers and magnocellular neurons in the paraventricular nucleus of rat hypothalamus. | dual antigen immunocytochemical staining procedures were used in the same tissue section to determine the distribution of acth immunostained fibers and varicosities within the magnocellular and parvocellular divisions in the paraventricular nucleus (pvn) of rat hypothalamus and elucidate its anatomical relationship to vasopressin (vp) and oxytocin (oxy)-containing neurons. double immunostained preparations using glucose oxidase-antiglucose oxidase complex combined with pap complex to visualize t ... | 1985 | 3001668 |
| virulence genes regulated at the transcriptional level by ca2+ in yersinia pestis include structural genes for outer membrane proteins. | yersinia pestis, the causative agent of plague, has a virulence determinant called the low-ca2+ response (lcr+ phenotype) that confers on the bacterium ca2+ dependence for growth at 37 degrees c and expression of v antigen. this virulence determinant is common to the three species of yersinia and is mediated by lcr plasmids (called pcd in y. pestis). in this study, we generated insertions of mu di1(ap lac) in pcd1 of y. pestis kim, screened for cells showing transcriptional regulation by ca2+, a ... | 1986 | 3002984 |
| cloning of the bacillus subtilis dlg beta-1,4-glucanase gene and its expression in escherichia coli and b. subtilis. | the gene encoding beta-1,4-glucanase in bacillus subtilis dlg was cloned into both escherichia coli c600sf8 and b. subtilis psl1, which does not naturally produce beta-1,4-glucanase, with the shuttle vector ppl1202. this enzyme is capable of degrading both carboxymethyl cellulose and trinitrophenyl carboxymethyl cellulose, but not more crystalline cellulosic substrates (l. m. robson and g. h. chambliss, appl. environ. microbiol. 47:1039-1046, 1984). the beta-1,4-glucanase gene was localized to a ... | 1986 | 3003033 |
| reactivation of latent murine cytomegalovirus from kidney. | cytomegalovirus (cmv) is presumed to cause latent infection, but the sites of infection are incompletely known. we propose that latent murine cytomegalovirus is present in kidney and may be reactivated by explanation. immunosuppressive agents and allogeneic stimuli may enhance this process. balb/c mice were infected 11 to 14 months previously with 10(5) pfu of smith strain murine cytomegalovirus intraperitoneally. kidneys from 15 infected and nine uninfected mice were washed, minced into 1 to 2 ... | 1985 | 3003444 |
| [the afferent pathway of the hippocampus on participation in acupuncture analgesia. a study of retrograde transport method]. | | 1985 | 3004787 |
| [forestomach carcinoma and hepatoma induced in mice and rats by n-3-methylbutyl-n-1-methylacetonylnitrosamine (mambna)]. | mambna is a new n-nitroso compound isolated from fusarium moniliforme-inoculated corn meal. in the present studies the carcinogenicity of mambna is shown by the induction of forestomach carcinomas and liver tumors in mice and rats following the gastric intubation of this compound. among 42 mice of kunming (km) stock treated with mambna (10-20 mg/week), 22 showed epithelial hyperplasia of the esophagus, 29 papillomas and 4 squamous carcinomas in the forestomach, and there were 6 liver adenomas an ... | 1985 | 3004854 |
| bradykinin stimulates gtp hydrolysis in ng108-15 membranes by a high-affinity, pertussis toxin-insensitive gtpase. | in membranes of neuroblastoma x glioma hybrid (ng108-15) cells, bradykinin (ec50 approximately equal to 5 nm) stimulates gtp hydrolysis by a high-affinity gtpase (km approximately equal to 0.2 microm). the octapeptide, des-arg9-bradykinin, was inactive. stimulation of gtp hydrolysis by bradykinin and an opioid agonist was partially additive. treatment of ng108-15 cells with pertussis toxin, which inactivates ni, eliminated gtpase stimulation by the opioid agonist but not by bradykinin. the data ... | 1986 | 3005034 |
| bradykinin stimulates gtp hydrolysis in ng108-15 membranes by a high-affinity, pertussis toxin-insensitive gtpase. | in membranes of neuroblastoma x glioma hybrid (ng108-15) cells, bradykinin (ec50 approximately equal to 5 nm) stimulates gtp hydrolysis by a high-affinity gtpase (km approximately equal to 0.2 microm). the octapeptide, des-arg9-bradykinin, was inactive. stimulation of gtp hydrolysis by bradykinin and an opioid agonist was partially additive. treatment of ng108-15 cells with pertussis toxin, which inactivates ni, eliminated gtpase stimulation by the opioid agonist but not by bradykinin. the data ... | 1986 | 3005034 |
| nucleotide sequence of the central non-transcribed spacer region of physarum polycephalum rdna. | in physarum polycephalum the rrna genes are present on linear extrachromosomal molecules, each containing two transcription units arranged as a giant palindrome. in the center of the molecule, between the two transcription units, is the 23-kb central spacer, previously shown to contain the replication origins and several regions of direct and inverted repeats. segments of all the repeats in the spacer have been sequenced and their overall organization determined. the entire spacer consists of re ... | 1985 | 3005112 |
| in vitro binding of an ige protein to human platelets. | bronchoconstriction in extrinsic asthma is initiated by mediators released from ige-sensitized leukocytes after contact with polyvalent antigen. because platelets also contain soluble mediators that can cause bronchoconstriction, platelet activation and release of the contents of platelet granules may play a role in ige-mediated responses under some circumstances. we therefore sought to determine if platelets are capable of binding ige and if cross-linking this cell-bound ige initiates secretion ... | 1986 | 3007618 |
| genetic basis for altered pathogenesis of an immune-selected antigenic variant of reovirus type 3 (dearing). | in this paper we provide a step by step comparison of the pathogenesis of murine infection caused by reovirus type 3 (dearing) and an antigenic variant (k) selected by its resistance to neutralization with a monoclonal antibody (g5) directed against the t3 hemagglutinin. to show that specific changes in the biologic properties of variant k were due to mutation in the s1 double-stranded rna segment (gene), which encodes the viral hemagglutinin, we generated a reassortant virus ("1 ha k") containi ... | 1986 | 3012122 |
| biochemical and antigenic characterization of feline herpesvirus-1-like isolates from dogs. | the dna and polypeptide patterns of feline herpesvirus-1 (fhv-1), a virus usually associated with feline respiratory infections, were compared to those of five herpesvirus isolates from dogs. these canine isolates had been shown to be antigenically similar to fhv-1 by cross neutralization tests. dna from fhv-1 (c-27 strain) and each canine isolate was digested with either bam hi, eco r 1 or sal i and analyzed on 0.8 per cent agarose gels. the restriction digest patterns of the canine isolates we ... | 1986 | 3013133 |
| membrane receptors for retinol-binding protein in cultured human retinal pigment epithelium. | the retinal pigment epithelium (rpe) is responsible for transport of retinol from the choroidal circulation to the photoreceptors. in the intact eye, this process is mediated by membrane receptors for plasma retinol binding protein (rbp) distributed basolaterally on the rpe cells. we have shown that cultured human rpe expresses this receptor. a binding curve exhibiting saturation was generated by incubating enzymatically detached epithelial sheets with increasing concentrations of 125i-labelled ... | 1986 | 3013795 |
| membrane receptors for retinol-binding protein in cultured human retinal pigment epithelium. | the retinal pigment epithelium (rpe) is responsible for transport of retinol from the choroidal circulation to the photoreceptors. in the intact eye, this process is mediated by membrane receptors for plasma retinol binding protein (rbp) distributed basolaterally on the rpe cells. we have shown that cultured human rpe expresses this receptor. a binding curve exhibiting saturation was generated by incubating enzymatically detached epithelial sheets with increasing concentrations of 125i-labelled ... | 1986 | 3013795 |
| molecular cloning and restriction endonuclease mapping of the rat cytomegalovirus genome. | rat cytomegalovirus (rcmv) dna was cleaved by restriction endonuclease ecori into 24 fragments ranging in mol. wt. from 34 x 10(6) to 0.20 x 10(6), of which 18 fragments could be cloned in plasmid pacyc 184. restriction endonuclease xbai cleaved the rcmv genome into 28 fragments, ranging in size from 44 x 10(6) to 0.81 x 10(6), of which 24 fragments were cloned in plasmid psp62-pl. among the restriction fragments that could not be cloned were two major terminal colinear fragments, ecori-a (34 x ... | 1986 | 3014047 |
| expression of simian virus 40 early and late genes in mouse oocytes and embryos. | simian virus 40 (sv40) large- and small-tumor antigens (t-ag, t-ag) are normally synthesized early after infection of either permissive (monkey) or nonpermissive (mouse) fibroblasts, whereas an equivalent amount of viral coat protein (v-ag) is observed late after infection of permissive cells and only after viral dna replication has occurred. to determine whether or not expression of these genes is regulated in the same manner during early mammalian development, sv40 dna was injected into the nu ... | 1986 | 3016324 |
| distinct pathways of viral spread in the host determined by reovirus s1 gene segment. | the genetic and molecular mechanisms that determine the capacity of a virus to utilize distinct pathways of spread in an infected host were examined by using reoviruses. both reovirus type 1 and reovirus type 3 spread to the spinal cord following inoculation into the hindlimb or forelimb footpad of newborn mice. for type 3 this spread is through nerves and occurs via the microtubule-associated system of fast axonal transport. by contrast, type 1 spreads to the spinal cord through the bloodstream ... | 1986 | 3016895 |
| [experimental nephritis induced by coxsackie b4 virus in mice--transient mesangial proliferation associated with acute viremia]. | | 1986 | 3018334 |
| construction and characterisation of a series of multi-copy promoter-probe plasmid vectors for streptomyces using the aminoglycoside phosphotransferase gene from tn5 as indicator. | several versatile, multi-copy, promoter-probe plasmid vectors have been constructed that replicate in a wide range of streptomyces species. transcriptional activity is detected by the expression of a promoter-less aminoglycoside phosphotransferase gene (neo) derived from the transposon tn5; expression of this gene confers kanamycin and neomycin resistance on streptomyces lividans. an efficient transcriptional terminator from e. coli phage fd has been inserted upstream of the neo coding region to ... | 1986 | 3018431 |
| testing for htlv-iii/lav antibody. | | 1986 | 3019171 |
| [ecology and epidemiology of transmissible viruses of sandflies in italy]. | | 1986 | 3019208 |
| susceptibility to varicella-zoster virus among adults at high risk for exposure. | the adult health care provider who is susceptible to varicella zoster virus (vzv) represents a risk to her or himself and to patients. nineteen percent of employees at this children's hospital had no or uncertain prior experience with vzv, and of these, 28% were found to be vzv susceptible, representing 5% of the total population of 2,730 hospital employees. during the 12 months of study, six of the potential 135 to 137 vzv-susceptible individuals acquired varicella. vzv-susceptible health care ... | 1986 | 3019915 |
| susceptibility to varicella-zoster virus among adults at high risk for exposure. | the adult health care provider who is susceptible to varicella zoster virus (vzv) represents a risk to her or himself and to patients. nineteen percent of employees at this children's hospital had no or uncertain prior experience with vzv, and of these, 28% were found to be vzv susceptible, representing 5% of the total population of 2,730 hospital employees. during the 12 months of study, six of the potential 135 to 137 vzv-susceptible individuals acquired varicella. vzv-susceptible health care ... | 1986 | 3019915 |
| inhibition and reversal of endotoxin-, aggregated igg- and paf-induced hypotension in the rat by sri 63-072, a paf receptor antagonist. | platelet activating factor (paf) given intravenously produces systemic hypotension in the rat. similar effects can be induced using endotoxin or heat-aggregated igg challenges, which are thought to involve endogenous paf release. extending this concept, we have examined the ability of the paf antagonist sri 63-072 to inhibit or reverse systemic hypotension induced with paf, heat-aggregated igg or endotoxin 0111-b4 in rats. at 100 ng kg-1 paf, there occurred a 38.6 +/- 5.1% decrease in carotid me ... | 1986 | 3019921 |
| inhibition and reversal of endotoxin-, aggregated igg- and paf-induced hypotension in the rat by sri 63-072, a paf receptor antagonist. | platelet activating factor (paf) given intravenously produces systemic hypotension in the rat. similar effects can be induced using endotoxin or heat-aggregated igg challenges, which are thought to involve endogenous paf release. extending this concept, we have examined the ability of the paf antagonist sri 63-072 to inhibit or reverse systemic hypotension induced with paf, heat-aggregated igg or endotoxin 0111-b4 in rats. at 100 ng kg-1 paf, there occurred a 38.6 +/- 5.1% decrease in carotid me ... | 1986 | 3019921 |
| [biochemistry of amino acid derivatives of [103ru] ruthenocene. comparison with 131i hippuran]. | the potential radiopharmaceuticals: ruthenocenoyl alanine, ruthenocenoyl methionine, 1'-methyl-ruthenocenoyl glycine and its esters were labelled with 103ru starting from the analogous ferrocene compounds. in a series of tests in mice and rats these substances were compared with hippuran and ruppuran (= ruthernocenoyl glycine, a ruthenocene-amino acid analogue of hippuran). the organ distribution of these compounds was measured at various times after injection. kidney concentrations of 1'-methyl ... | 1986 | 3019949 |
| [biochemistry of amino acid derivatives of [103ru] ruthenocene. comparison with 131i hippuran]. | the potential radiopharmaceuticals: ruthenocenoyl alanine, ruthenocenoyl methionine, 1'-methyl-ruthenocenoyl glycine and its esters were labelled with 103ru starting from the analogous ferrocene compounds. in a series of tests in mice and rats these substances were compared with hippuran and ruppuran (= ruthernocenoyl glycine, a ruthenocene-amino acid analogue of hippuran). the organ distribution of these compounds was measured at various times after injection. kidney concentrations of 1'-methyl ... | 1986 | 3019949 |
| frostbite: experimental assessment of tissue damage using tc-99m pyrophosphate. work in progress. | we designed an experimental model using a new method of freezing to study the pathogenesis and treatment of frostbite. frostbite was simulated in a manner that closely resembles that which occurs in a natural environment. we used a radionuclide imaging technique to monitor the evolution and extent of tissue damage relative to temperature, rate of freezing, and controlled rewarming. characteristic sequential changes were demonstrated on sequential nuclear scans. nonperfusion, followed by perfusio ... | 1986 | 3020608 |
| exposure of sheep to natural aerosols of foot-and-mouth disease virus. | sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ... | 1986 | 3020658 |
| a low-ca2+ response operon encodes the v antigen of yersinia pestis. | yersinia pestis has a virulence regulon called the low-ca2+ response that is mediated by the plasmid pcd and manifested as regulation of growth and of expression of several virulence-associated properties by ca2+ and temperature. we used mu di(ap lac) to obtain a mutation in pcd1 of y. pestis kim that rendered the bacteria unable to express one of these properties, the v antigen. this mutant also had lost the ca2+ requirement for growth at 37 degrees c and was avirulent in mice. two-dimensional ... | 1986 | 3021629 |
| pain, increasing girth in healthy japanese man. | | 1986 | 3021791 |
| pain, increasing girth in healthy japanese man. | | 1986 | 3021791 |
| [structural polymorphism of the provirus integrated in the genome of mammalian cells transformed by rous sarcoma virus]. | the structural organization of integrated rous sarcoma virus (rsv) genome in 8 different mammalian tumour cell lines has been studied. the different types of provirus rearrangements were found, e.g.: breakpoint mutations, deletions of rsv structural genes, elimination of the entire viral genome. the structural changes of proviruses appeared during long-term cultivation of tumour cell lines in vitro. the ability of transformed cells to produce complete infectious rsv correlate with the structural ... | 1986 | 3022121 |
| [structural polymorphism of the provirus integrated in the genome of mammalian cells transformed by rous sarcoma virus]. | the structural organization of integrated rous sarcoma virus (rsv) genome in 8 different mammalian tumour cell lines has been studied. the different types of provirus rearrangements were found, e.g.: breakpoint mutations, deletions of rsv structural genes, elimination of the entire viral genome. the structural changes of proviruses appeared during long-term cultivation of tumour cell lines in vitro. the ability of transformed cells to produce complete infectious rsv correlate with the structural ... | 1986 | 3022121 |
| partial transformation of mouse fibroblastic and epithelial cell lines with the v-myc oncogene. | to investigate the role of the myc gene in mammalian cell transformation, plasmid constructs containing the v-myc oncogene and a co-selectable g418 resistance marker were introduced into both mouse fibroblasts (nih-3t3) and bladder epithelial cells (bbn3 and bbn7). after transfection or microinjection of dna, no transformed foci could be detected on confluent monolayers but, when the cells were cultured under conditions in which individual cells were allowed to grow and form colonies, morphologi ... | 1987 | 3023119 |
| in vitro and in vivo inhibition of cysteine proteinases by est, a new analog of e-64. | e-64 isolated from a culture of aspergillus japonicus is a specific inhibitor of cysteine proteinases. e-64-c, a synthetic analog of e-64, was effective in model animals of muscular dystrophy only when it was given intraperitoneally and by means of osmotic minipump. it showed no effects due to its low absorbability from intestine when it was administered orally. est, the ethyl ester of e-64-c, was expected to be readily absorbed through intestinal membrane, since it is more lipophilic than e-64- ... | 1986 | 3023601 |
| [isolation of yersinia pestis plasmids with transposon markers]. | transposons tn1, tn7, tn9, tn10 have been inserted into each of three known plasmids in yersinia pestis and have been shown to mutagenize the different plasmid genes. the marked plasmids are shown to be transduced by bacteriophage p1 cml clr 100 ts in intrageneric crosses. the genes of 61-65 md plasmid were found to be impaired with high frequencies by tn9 and tn10 insertions blocking the synthesis of fraction i antigen. the genes are also impaired in course of transduction of transposon marked ... | 1985 | 3025677 |
| [mutagenic effect during transduction of (gm-km)r markers of the r323 plasmid in yersinia pestis]. | genes of resistance to some aminoglycoside antibiotics from plasmid r323 were transduced by bacteriophage p1 cml clr100 ts in yersinia pestis. the resistance markers were capable of insertion into the chromosome or plasmid in the recipient cells causing the mutagenic effect. the results obtained suggest the transposon nature of plasmid fragment coding for gentamicin-kanamycin resistance. | 1985 | 3025695 |
| [restriction map of pesticinogenicity plasmid pyp1 of yersinia pestis]. | the restriction map of yersinia pestis pesticinogenicity plasmid pyp1 has been constructed with the use of 18 restriction endonucleases. plasmid dimensions (6.3 md) have been specified, the genes for pesticin synthesis, for pesticin immunity protein, fibrinolysin and plasmocoagulase have been localized by molecular cloning of single plasmid dna fragments in vector plasmid pbr322. | 1986 | 3025701 |
| identification of a conserved domain among cyclic nucleotide phosphodiesterases from diverse species. | partial amino acid sequences have been determined for the ca2+/calmodulin-stimulated cyclic nucleotide phosphodiesterase from bovine brain and the cgmp-stimulated cyclic nucleotide phosphodiesterase from bovine heart. examination of these sequences for homologous segments and comparison with protein sequences derived from the nucleotide sequences of the yeast pde2 gene and the drosophila dunce+ gene [chen, c.-n., denome, s. & davis, r. l. (1986) proc. natl. acad. sci. usa 83, 9313-9317; sass, p. ... | 1986 | 3025833 |
| phenotypic characterisation of peripheral blood lymphoid cells in people exposed to fibrous zeolite. | among inhabitants of the village of karain in turkey there is an extremely high incidence of malignant mesothelioma, most probably due to exposure to erionite, which is a fibrous zeolite and similar in appearance and properties to asbestos. this mineral may be found in the dust in the village. to characterise possible disturbances in the immune system of people exposed to fibrous zeolite, a phenotypic characterisation of lymphoid cells in the peripheral blood of 74 immigrants to sweden from kara ... | 1986 | 3026434 |
| phenotypic characterisation of peripheral blood lymphoid cells in people exposed to fibrous zeolite. | among inhabitants of the village of karain in turkey there is an extremely high incidence of malignant mesothelioma, most probably due to exposure to erionite, which is a fibrous zeolite and similar in appearance and properties to asbestos. this mineral may be found in the dust in the village. to characterise possible disturbances in the immune system of people exposed to fibrous zeolite, a phenotypic characterisation of lymphoid cells in the peripheral blood of 74 immigrants to sweden from kara ... | 1986 | 3026434 |
| primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen. | the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ... | 1986 | 3028680 |
| primary biliary cirrhosis: assessment of the quantitative importance of the adenine nucleotide translocator protein as a major mitochondrial antigen. | the adenine nucleotide translocator protein (ant) is the first well-characterized mitochondrial polypeptide to be identified as an antigen for antimitochondrial autoantibodies (ama) in pbc sera. because of the potential use for a highly purified antigen as a tool in studying the aetiology of pbc, we have undertaken an assessment of the quantitative importance of ant as a pbc-specific mitochondrial antigen. immunoblotting and elisa techniques were used. both methods reveal pbc antibodies against ... | 1986 | 3028680 |
| [3h]cpp, a new competitive ligand for nmda receptors. | | 1986 | 3028828 |
| [3h]cpp, a new competitive ligand for nmda receptors. | | 1986 | 3028828 |
| the insulin receptor tyrosyl kinase phosphorylates holomeric forms of the guanine nucleotide regulatory proteins gi and go. | an affinity purified human insulin receptor preparation was shown to phosphorylate the alpha- and beta-subunits of the guanine nucleotide-regulatory proteins gi and go, derived from bovine brain. the presence of insulin stimulated the rate of their phosphorylation some 2-fold. the presence of gi and go did not affect the degree of autophosphorylation of the beta-subunit of the insulin receptor. under conditions known to cause the dissociation of gi and go into their constituent subunits then pho ... | 1987 | 3028864 |
| the insulin receptor tyrosyl kinase phosphorylates holomeric forms of the guanine nucleotide regulatory proteins gi and go. | an affinity purified human insulin receptor preparation was shown to phosphorylate the alpha- and beta-subunits of the guanine nucleotide-regulatory proteins gi and go, derived from bovine brain. the presence of insulin stimulated the rate of their phosphorylation some 2-fold. the presence of gi and go did not affect the degree of autophosphorylation of the beta-subunit of the insulin receptor. under conditions known to cause the dissociation of gi and go into their constituent subunits then pho ... | 1987 | 3028864 |
| on the fidelity of dna replication: herpes dna polymerase and its associated exonuclease. | procaryotic dna polymerases contain an associated 3'----5' exonuclease activity which provides a proofreading function and contributes substantially to replication fidelity. dna polymerases of the eucaryotic herpes-type viruses contain similar associated exonuclease activities. we have investigated the fidelity of polymerases purified from wild type herpes simplex virus, as well as from mutator and antimutator strains. on synthetic templates, the herpes enzymes show greater relative exonuclease ... | 1987 | 3029700 |
| cdna cloning of human myeloperoxidase: decrease in myeloperoxidase mrna upon induction of hl-60 cells. | myeloperoxidase (mpo), the most abundant neutrophil protein, is a bacteriocidal component of the primary granules and a critical marker in distinguishing acute myelogenous leukemia from acute lymphoid leukemia. a cdna clone for human mpo was isolated by immunologic screening of human hematopoietic lambda gt11 expression vector libraries with specific anti-mpo antibody. the identity of the cdna clone was confirmed by finding that epitope-selected antibody against this clone recognizes purified mp ... | 1987 | 3031662 |
| cdna cloning of human myeloperoxidase: decrease in myeloperoxidase mrna upon induction of hl-60 cells. | myeloperoxidase (mpo), the most abundant neutrophil protein, is a bacteriocidal component of the primary granules and a critical marker in distinguishing acute myelogenous leukemia from acute lymphoid leukemia. a cdna clone for human mpo was isolated by immunologic screening of human hematopoietic lambda gt11 expression vector libraries with specific anti-mpo antibody. the identity of the cdna clone was confirmed by finding that epitope-selected antibody against this clone recognizes purified mp ... | 1987 | 3031662 |
| membrane conductance and potassium permeability of the rat lens. | the membrane potential, electrical impedance and 86rb+ efflux rate constants were measured in the rat lens perifused at 35 degrees c. the membrane conductance was obtained from the difference between the magnitude of the impedance at low (less than 0.1 hz) and high (greater than 100 hz) frequencies. values of the rubidium permeability coefficients (prb) were obtained from the rate constant and potential data. the values for the membrane potential and conductance in control solution (5 mm potassi ... | 1987 | 3031720 |
| membrane conductance and potassium permeability of the rat lens. | the membrane potential, electrical impedance and 86rb+ efflux rate constants were measured in the rat lens perifused at 35 degrees c. the membrane conductance was obtained from the difference between the magnitude of the impedance at low (less than 0.1 hz) and high (greater than 100 hz) frequencies. values of the rubidium permeability coefficients (prb) were obtained from the rate constant and potential data. the values for the membrane potential and conductance in control solution (5 mm potassi ... | 1987 | 3031720 |
| [detection of an inhibitor of the causative agent of plague active against strains of the plague microbe from an homologous focus. the effect of lithium chloride on detecting the inhibitor]. | a nutrient medium containing 0.7 per cent of lithium chloride was tested and yersinia pestis inhibitor was detected in 83.3 per cent of the strains on the growth tufts of 8 indicator strains isolated from a homologous focus. lowering the lithium chloride concentration in the medium up to 0.6 per cent promoted detection of the inhibitor in a larger number of the tested strains (93.3 per cent). but even on this medium none of the indicator strains formed overall growth tufts. the subcultures of th ... | 1987 | 3032085 |
| [effect of cholera enterotoxin on carbohydrate metabolism of the liver and small intestine mucosa in the rabbit]. | changes in carbohydrate metabolism were studied in the isolated intestinal loops of rabbits during secretory diarrhea, induced by cholera enterotoxin. glucose synthesis level in the small intestinal mucosa and liver was measured by isotope technique, using l-alanine as a precursor. intestinal gluconeogenesis, calculated per mg of protein, appeared to be twice higher than in the liver of fasting rabbits. cholera enterotoxin administration enhanced gluconeogenesis in the liver by 60%, as compared ... | 1987 | 3032299 |
| calmodulin in porcine malignant hyperpyrexia. | the anaesthetic complication malignant hyperpyrexia (mh) is due to an elevation of the myoplasmic ca2+ concentration. examination of calmodulin isolated from mh susceptible swine suggests that the disorder in calcium regulation in mh is not due to an abnormality in calmodulin. | 1987 | 3032704 |
| the mechanisms of antiviral immunity induced by a vaccinia virus recombinant expressing herpes simplex virus type 1 glycoprotein d: clearance of local infection. | we have shown that immunization of mice with a vaccinia virus recombinant expressing glycoprotein d of herpes simplex virus (hsv)-1 will induce a variety of l3t4+ t cell responses. these included a hsv-specific delayed-type hypersensitivity response, t cell help for the induction of antiviral antibodies, and the ability to eliminate a challenge dose of hsv from the pinna. this protection against a subcutaneous virus challenge was not mediated by the delayed-type hypersensitivity response because ... | 1987 | 3033075 |
| more exact quantification of interleukin-2 production by addition of anti-tac monoclonal antibody to cultures of stimulated lymphocytes. | human t-cells produce interleukin-2 (il-2) in response to in vitro stimulation with mitogens and antigens. precise measurement of il-2 production in vitro is hampered by binding of il-2 to il-2 receptors on activated t-cells which results in il-2 consumption. in an attempt to prevent il-2 consumption, we supplemented the cultures with anti-tac, a monoclonal antibody that functionally blocks the human lymphocyte receptor for il-2. addition of anti-tac to cultures of mitogen-stimulated peripheral ... | 1987 | 3033083 |
| detection of antigen to herpes simplex virus in cerebrospinal fluid from patients with herpes simplex encephalitis. | cerebrospinal fluid (csf) specimens were obtained from patients with presumed herpes simplex encephalitis who underwent brain biopsy for diagnostic confirmation. coded csf specimens were fixed on nitrocellulose filter paper and probed with a pool of monoclonal antibodies directed against four herpes simplex virus glycoproteins (gb, gc, gd, and ge). herpes simplex virus antigen was detected in 35 of 40 specimens obtained from 26 biopsy-positive patients. in contrast, only three of 25 specimens fr ... | 1987 | 3033092 |
| effects of inhibitors of the cytoplasmic structures and functions on the early phase of infection of cultured cells with simian virus 40. | to obtain information about cytoplasmic structures and functions involving the entry of simian virus 40 virions into cells, we examined whether the inhibitors that affect the functions and/or structure of lysosomes, cell membrane, and cytoskeletons inhibit expression of nuclear t antigen in the sv40-inoculated rat 3y1 and monkey cv-1 cells. chloroquine, methylamine, and butylamine did not inhibit t-antigen expression, suggesting that lysosomal acidification is not required for establishment of i ... | 1987 | 3033894 |
| maturation antigen of the mouse sperm flagellum: ii. origin from holocrine cells of the distal caput epididymis. | during epididymal transit, the mouse sperm flagellum acquires a surface glycoprotein (sma4) from epididymal fluid that functions as a sperm antiagglutinin. to determine the origin of this molecule, testes and epididymides of male mice were sectioned for light microscopy and stained with wheat germ agglutinin (wga)-peroxidase, a probe that has been used previously to examine the biology of sma4. wga reactivity was localized to the cytoplasm in a small population of cells in the distal caput epidi ... | 1987 | 3034104 |
| metabolism of nicotinamide mononucleotide in beef liver. | nicotinamide mononucleotide (nmn) is not only an intermediate for the biosynthesis but also a degradation product of pyridine cofactors in animal tissues. among the animal tissues tested, the highest nmn catabolizing activity was detected in beef liver (5.6 mumol/min/g tissue). this activity was 16 times higher than the nad hydrolysis catalyzed by the liver nad glycohydrolase. as a result of enzymatic analysis of the nmn splitting process, two types of enzyme responsible for this catabolism were ... | 1987 | 3034159 |
| the effect of cycloleucine on sfv a7(74) infection in mice. | cycloleucine (cl), a non-metabolizable amino acid analogue, was found to reduce thymus and spleen weights in semliki forest virus (sfv) strain a7(74) infected and control mice. the maximum effects were seen when three daily doses of cl were given to mice 24 h after an sfv a7(74) infection. in these mice thymus atrophy led to abolition of thymus dependent immune responses and changes in the pathological features of the viral infection--the most striking feature being prevention of demyelination. ... | 1987 | 3034321 |
| effects of procaine on pharmaco-mechanical coupling mechanisms activated by acetylcholine in smooth muscle cells of porcine coronary artery. | the action of procaine on pharmaco-mechanical coupling activated by application of acetylcholine (ach) was investigated using collagenase-treated dispersed intact and skinned smooth muscle cells and intact muscle tissues of the porcine coronary artery. ach reduced stored 45ca2+, and this action was prevented by procaine in intact dispersed cells. the maximum reduction in the level of stored 45ca induced by caffeine (25 mm) or inositol 1,4,5-trisphosphate (insp3; 3 microm) was also prevented by p ... | 1987 | 3034448 |
| open-chest cardiac massage after closed-chest compression in a canine model: when to intervene. | open-chest cardiac massage appears beneficial in improving hemodynamics during resuscitation efforts and in improving resuscitation success. the time between cardiac arrest and the initiation of open-chest cardiac massage is crucial. it would appear that if initiation of open chest cardiac massage is delayed for more than 20 min from the onset of cardiac arrest, little or no successful outcome can be expected. further techniques for assessing the adequacy of closed-chest compression cpr are need ... | 1987 | 3035670 |
| procollagenase activator produced by rabbit uterine cervical fibroblasts. | culture medium from rabbit uterine cervical fibroblasts contained a procollagenase and a neutral proproteinase which acts as a procollagenase activator. these two proenzymes have been purified by a combination of ion-exchange, affinity and gel chromatographies. the purified neutral proproteinase showed mr 60,000 with sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. this neutral proproteinase was activated by trypsin, 4-aminophenylmercuric acetate (apma) and plasmin, and the active spe ... | 1987 | 3036065 |
| a factor discriminating between the wild-type and a mutant polyomavirus enhancer. | enhancers increase the frequency of transcription initiation from linked promoter elements, most probably as a result of the binding of specific proteins to the enhancer. the polyomavirus early region is expressed in differentiated mouse cells but not in undifferentiated embryonal carcinoma (ec) cells. this host range is a function of the enhancer because polyomavirus mutants selected for growth in ec cells have mutations in the enhancer and the host range is reproduced in transfection assays us ... | 1987 | 3037380 |
| the pharmacokinetics of proguanil in human subjects following a single oral dose. | the pharmacokinetics of orally administered 200-mg dose of proguanil in volunteers, one african and one caucasian, is described. the drug was rapidly absorbed reaching a peak concentration in the blood within 3 h, and declining slowly thereafter to give a terminal phase elimination half life of 11.20 +/- 4.10 h and a systemic clearance of 1.270 +/- 0.020 l/h/kg. the small apparent volume of distribution shows that the drug is confined mainly to the blood and is not extensively bound to tissues; ... | 1987 | 3037875 |
| cloning in escherichia coli of the gene specifying the dna-entry nuclease of bacillus subtilis. | with the aim of cloning genes involved in transformation of bacillus subtilis, a set of transformation-deficient mutants was isolated by means of insertional mutagenesis with plasmid phv60 (vosman et al., 1986). analysis of these mutants showed that those mapping in the aroi region lacked the dna-entry nuclease activity. plasmid phv60 derivatives, containing flanking chromosomal dna fragments, were isolated from these mutants and were used to screen a library of b. subtilis chromosomal dna in ph ... | 1987 | 3038682 |
| cloning in escherichia coli of the gene specifying the dna-entry nuclease of bacillus subtilis. | with the aim of cloning genes involved in transformation of bacillus subtilis, a set of transformation-deficient mutants was isolated by means of insertional mutagenesis with plasmid phv60 (vosman et al., 1986). analysis of these mutants showed that those mapping in the aroi region lacked the dna-entry nuclease activity. plasmid phv60 derivatives, containing flanking chromosomal dna fragments, were isolated from these mutants and were used to screen a library of b. subtilis chromosomal dna in ph ... | 1987 | 3038682 |
| antagonist effects of the enantiomers of 3-ppp towards alpha 1-adrenoceptors coupled to inositol phospholipid breakdown in the rat cerebral cortex. | the effects of the two enantiomers of 3-ppp upon alpha 1-adrenergic and muscarinic receptors coupled to the inositol phospholipid (pi) breakdown response have been investigated. 3-ppp(-) and 3-ppp(+) were found to antagonize the noradrenaline (10 microm)-stimulated pi breakdown in rat cerebral cortical miniprisms with ic50 values of 18 and 61 microm, respectively. the dopamine receptor antagonists haloperidol and raclopride were also antagonists, with ic50 values of 0.4 and 25 microm, respective ... | 1987 | 3039482 |
| fulminant leukemic polyradiculoneuropathy in a case of b-cell prolymphocytic leukemia. a clinicopathologic report. | a 52 year old man with a 10-month history of b-cell prolymphocytic leukemia (pll) died of an apparent acute fulminant polyradiculoneuropathy, a condition generally attributed to paraneoplastic complication. the pathologic examination disclosed diffuse leukemic infiltrations of the peripheral nervous system. it is suggested that this particularly aggressive form of b-cell chronic prolymphocytic leukemia presented a constellation of features that promoted the invasion of the peripheral nervous sys ... | 1987 | 3040217 |
| analysis of transfer genes and gene products within the trab-trac region of the escherichia coli fertility factor, f. | a series of plasmids that carry overlapping segments of f dna encoding the genes in the trab-trac interval was constructed, and a restriction enzyme map of the region was derived. plasmids carrying deletions that had been introduced at an hpai site within this interval were also isolated. the ability of these plasmids to complement transfer of f lac plasmids carrying mutations in trab, trav, and traw, and trac was analyzed. the protein products of the plasmids were labeled in uv-irradiated cells ... | 1987 | 3040671 |
| identification of encapsulated and non-encapsulated yersinia pestis by immunofluorescence tests using polyclonal and monoclonal antibodies. | rabbit polyclonal hyperimmune antibodies to yersinia pestis, and a mouse monoclonal antibody against the capsular antigen fraction 1 (f1) were compared in immunofluorescence (if) tests. fluorescent antibody conjugates were prepared from polyclonal antisera to four f1 positive y. pestis strains; the conjugated antibody to strain a1122 gave the strongest if staining of f1 positive and f1 negative y. pestis strains. indirect assays were rejected in favour of direct assays utilizing polyclonal and m ... | 1988 | 3042439 |