| [effect of vitamins of the b group on the accumulation of complex antigens of dysentery bacteria in the process of deep cultivation]. | | 1985 | 2416151 |
| [comparative characteristics of antigenic conversion induced by shigella flexneri and escherichia coli 0129 phages]. | the interaction of s. flexneri converting phages pe5, p90 and fv with e. coli antigenic variant o129, e. coli o129 converting phage vb with the above antigenic variant and with s. flexneri y-variant was studied. phage pe5 and phage vb were found to induce the conversion of o-antigen in e. coli antigenic variant 0129 and in s. flexneri y-variant with the detection of antigens v and 7,8. phages p90 and fv induced no conversion of o-antigen. changes in the antigenic properties of convertants were c ... | 1985 | 2416154 |
| anti-hla-b27 antibodies in sera from patients with gram-negative bacterial infections. | several forms of seronegative polyarthritis are strongly associated with hla-b27, and a number of microorganisms have been implicated in the etiology of these diseases. to explain the association between hla-b27 and arthritis initiated by infection with these organisms, it has been proposed that there is immunologic cross-reactivity between the b27 molecule and 1 or more microbial antigens, and that this cross-reactivity leads to tolerance to such infection and/or to the production of anti-hla-b ... | 1986 | 2421739 |
| [effect of levamisole immunocorrective therapy on the dynamics of infectious o-antigenemia and the clinical manifestations in dysentery patients]. | the immunocorrecting agent levamisole decreases the duration of infectious o-antigenemia, determined in the o-aggregate hemagglutination test and in the enzyme immunoassay, essentially accelerates the progress of convalescence and considerably decreases the possibility of the prolonged relapsing course of the disease in comparison with common methods of treatment. | 1986 | 2422855 |
| the response of the lung to foreign compounds that produce free radicals. | | 1986 | 2423026 |
| the interaction of myelin basic protein with tubulin and the inhibition of tubulin carboxypeptidase activity. | tubulin carboxypeptidase was found to be inhibited by myelin basic protein in a concentration dependent manner. the inhibition was produced by the interaction between myelin basic protein with the substrate. as a consequence of this interaction, turbid insoluble aggregates were formed at either 5 degrees or 37 degrees c. the turbidity increased by increasing the myelin basic protein concentration and it reached a plateau at a molar ratio of myelin basic protein to tubulin dimer of about 6. at pl ... | 1986 | 2423083 |
| mechanism of rna redistribution of the 17,000g postmitochondrial supernatant after incubation at 37 degrees c and its impact on other related biochemical investigations. | evidence for stripping of ribosomes from rer in the 17,000g pms of the liver of the cancer patient was obtained in the 1.35 m region which is the region between the ser and rer. rna/protein ratios for the ser, 1.35 m region, and rer of 0.001, 0.083, and 0.235, respectively, for this liver are consistent with the degranulation of rer compared with rna/protein ratios for ser and rer from normal livers of 0.025 +/- 0.003, and 0.35 + 0.030, respectively. a rna/protein ratio of 0.235 was obtained for ... | 1986 | 2423101 |
| monoclonal antibodies specific for shigella flexneri lipopolysaccharides: clones binding to type i and type iii:6,7,8 antigens, group 6 antigen, and a core epitope. | monoclonal antibodies against the shigella flexneri lipopolysaccharide (lps) were generated in two fusions by using the myeloma cell line sp2/0 as a fusion partner with spleen cells from balb/c mice immunized with s. flexneri serotypes 1b and 3a bacteria. the antibodies were characterized by immunoblotting, enzyme-linked immunosorbent assay (elisa), hemagglutination, and coagglutination. four different types of monoclonal antibodies were isolated: antibodies specific for the core antigen of the ... | 1986 | 2424839 |
| shigella flexneri o-antigen epitopes: chemical and immunochemical analyses reveal that epitopes of type iii and group 6 antigens are identical. | in the present investigation we studied the nature of a shigella flexneri o antigen, the type iii antigen. no structural entity has yet been attributed to this antigen. phenol-water-extracted lipopolysaccharides (lpss) from all s. flexneri serotypes possessing either group 6 antigen or both type iii antigen and group 6 antigen were subjected to de-o-acetylation by weak alkali treatment. two-dimensional nuclear magnetic resonance spectra of serotype 3a native lps and de-o-acetylated lps were comp ... | 1986 | 2424840 |
| [nbt-test stimulation of neutrophilic granulocytes by bacterial preparations in healthy persons]. | | 1986 | 2425131 |
| changes in tonicity of perfusion medium cause prolonged opening of calcium channels of the rat chromaffin cells to evoke explosive secretion of catecholamines. | secretion of catecholamines (ca) from the isolated rat adrenal gland during and after perfusion with hypertonic krebs bicarbonate solution was studied. perfusion with hypertonic solution made by adding either 156 mm sodium chloride, choline chloride, arginine hydrochloride, sodium thiocyanate, or 312 mm sucrose had no effect on the spontaneous secretion of ca. the secretion evoked by splanchnic nerve stimulation (10 hz for 30 sec) and nicotine (2 micrograms) remained unaffected during perfusion ... | 1986 | 2427669 |
| human p85 glycoprotein bears three distinct epitopes defined by several monoclonal antibodies. | a glycoprotein of apparent mol. wt 85,000 isolated from human cells of b lineage by affinity to 50b4-igg immunoadsorbent was shown previously to express two spatially distinct epitopes identified with monoclonal antibodies 50b4 and 50e6 [letarte et al. (1985) molec. immun. 22, 113-124]. it is now demonstrated that the p85 glycoprotein is homologous to the f10-44-2 antigen, defined initially as a t-lymphocyte-granulocyte-brain antigen [dalchau et al. (1980) eur. j. immun. 10, 745-749], the a1g3 h ... | 1986 | 2427941 |
| hla-b27m1m2 and high immune responsiveness to shigella flexneri in post-dysenteric arthritis. | the heterogeneous hla-b27 antigen is closely associated with post-infectious or reactive arthritis (rea) and is comprised of two serologically defined variants: b27m1+m2+ and b27m1+m2-. an outbreak of dysentery (n = 120) caused by a shigella flexneri 2a strain, which possessed cell envelope antigens with epitopes resembling b27m2, resulted in five b27m1+m2+ patients with rea. the remaining seven b27m1+m2+, one b27m1+m2- and all but three b27-negative patients remained free of joint symptoms; the ... | 1986 | 2428743 |
| colonization, virulence, and mucosal interaction of an enteropathogenic escherichia coli (strain rdec-1) expressing shigella somatic antigen in the rabbit intestine. | the group factor 3,4 somatic antigen was transferred by recombination from a donor strain of shigella flexneri type 2a to the escherichia coli o15 rabbit pathogen strain rdec-1. a hybrid clone (7482-1-1) that expressed only the s. flexneri 3,4 somatic antigen and a second clone (7482-1-7) from the same mating mixture that expressed only the o15 somatic antigen were compared for virulence in rabbits. the 7482-1-1 strain produced diarrhea in 10% of rabbits versus 84% for the 7482-1-7 strain (p les ... | 1986 | 2430026 |
| induction in rats of lysine imbalance by dietary homoarginine. | the use of dietary homoarginine (ha) to produce a lysine imbalance was examined in young rats. ha, a basic amino acid analogue, was shown earlier to compete with lysine for transport across the blood-brain barrier. feeding a diet limiting in lysine and containing ha reduced food intake and growth; increasing dietary lysine content lessened these effects. a 3-fold increase in dietary lysine caused 10, 4, 20 and 50-fold increases in lysine concentrations in plasma, brain, liver and muscle, respect ... | 1986 | 2430077 |
| an activated harvey ras oncogene produces benign tumours on mouse epidermal tissue. | studies of the mutagenic action required for specific chemicals to produce benign or malignant tumours suggest that in mouse skin at least two genetic events occur before carcinoma formation. the isolation of an activated form of the c-rash gene from skin papillomas has provided evidence that this gene may be a target for the first mutation, which could constitute the initiating mutation in skin carcinogenesis. in vitro studies indicate that the v-rash gene of harvey murine sarcoma virus (ha-msv ... | 1986 | 2430189 |
| [synthesis of a repeating unit and the dimer of the repeating unit of the major chain of shigella flexneri o-antigen polysaccharide]. | methyl glycoside of the tetrasaccharide glcnac(beta 1-2)rha(alpha 1-2)rha(alpha 1-3)rha, which represents a repeating unit of the basic chain of shigella flexneri o-antigenic polysaccharides, was synthesized using acylated monosaccharide synthons. a dimer of the repeating unit, octasaccharide [glcnac(beta 1-2)rha(alpha 1-2) rha(alpha 1-3)rha(alpha 1-3)]2-ome was obtained by trclo4-catalyzed condensation of two tetrasaccharide blocks. | 1986 | 2430581 |
| lack of correlation between hla-b27 like antigenic epitopes on shigella flexneri and the occurrence of reactive arthritis. | | 1986 | 2430745 |
| [intergeneric antigenic relation of hafnia and shigella (s. flexneri and s. boydii) bacteria]. | the o-antigenic relationships between hafnia and shigellae (s. flexneri and s. boydii) have been studied. for the first time the presence of antigenic relationship between hafnia o19, o4, o9, o33, o5, o16, o12, o7, o29, o28, o10, o32, o24, o25, o18, o1, o13, o3, o22, o30, o37, o14, o11, o25, o23, o21, o28, o16, o24, o8, o26, o27 and s. flexneri la, lb, 2a, 2b, 4a, 4b, 6, 5a, 5b, as well as between hafnia o10, o21, o35, o36, o9, o28, o8, o30 and s. boydii 1, 3, 6, 14, 2, 5, 2, 12 have been reveal ... | 1986 | 2431565 |
| in situ hybridization methods for the detection of somatostatin mrna in tissue sections using antisense rna probes. | in situ hybridization studies with [32p] and [3h] labelled antisense rna probes were undertaken to determine optimal methods of tissue fixation, tissue sectioning, and conditions of hybridization, and to compare the relative merits of the two different radioactive labels. the distribution of somatostatin mrna in neurons of rat brain using a labelled antisense somatostatin rna probe was employed as a model for these studies. the highest degree of sensitivity for in situ hybridization was obtained ... | 1986 | 2435682 |
| monoclonal antibodies specific for shigella flexneri lipopolysaccharides: clones binding to type iv, v, and vi antigens, group 3,4 antigen, and an epitope common to all shigella flexneri and shigella dysenteriae type 1 stains. | monoclonal antibodies reactive with shigella flexneri o antigens were generated in both mouse and rat systems. antibody-producing hybridomas were screened in an enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides as antigens, and the epitope specificities were determined with a panel of lipopolysaccharides and synthetic o-antigen-specific glycoconjugates as antigens. to verify the specificity seen in the enzyme-linked immunosorbent assay, the antibodies were used in ag ... | 1987 | 2437036 |
| [genetic characteristics and trends in the spread of a new shigella flexneri subserovar with the antigenic formula iv:7,8]. | as the result of experiments with the conjugation of s. flexneri strains 4 belonging to an unusual subserovar (iv: 7,8) with escherichia coli donor strains k12 hfr c and hfr h, as well as experiments with converting phages iv and 7,8, this new subserovar of s. flexneri 4, similarly to other s. flexneri subserovars, was proved to be the y-variant of shigellae rendered lysogenic by the two above phages. the experiments also revealed that 97.9% of all s. flexneri strains 4 (iv: 7,8) under study pos ... | 1987 | 2437740 |
| the novel carbohydrate epitope l3 is shared by some neural cell adhesion molecules. | the monoclonal l3 antibody reacts with an n-glycosidically linked carbohydrate structure on at least nine glycoproteins of adult mouse brain. three out of the l3 epitope-carrying glycoproteins could be identified as the neural cell adhesion molecules l1 and myelin-associated glycoprotein, and the novel adhesion molecule on glia. expression of the l3 carbohydrate epitope is regulated independently of the protein backbone of these three glycoproteins. based on the observation that out of three fun ... | 1987 | 2438289 |
| characterization of five shigella flexneri variant y-specific monoclonal antibodies using defined saccharides and glycoconjugate antigens. | the structural domains of the shigella flexneri variant y o-antigen epitopes 3,4 have defied definition, despite knowledge of the structure of the linear polysaccharide chain of the lps molecule. the dual epitope designation of group antigen 3,4 is based on absorption data using polyvalent rabbit antisera. five monoclonal antibodies specific for the y antigen, generated after immunization of balb/c mice or lou/c rats, were selected on the basis of elisa by using well-characterized s. flexneri y ... | 1987 | 2438343 |
| structure and biology of shigella flexneri o antigens. | | 1987 | 2438412 |
| increase of enkephalin and decrease of substance p immunoreactivity in the dorsal and ventral striatum of the rat after midbrain 6-hydroxydopamine lesions. | unilateral 6-hydroxydopamine lesions of the mesostriatal dopaminergic system in the rat resulted in a decrease of substance p-immunoreactivity in the ventral striatum, and in a heterogeneously distributed increase of enkephalin-immunoreactivity in the dorsal and ventral striatum and the globus pallidus. the respective decrease and increase are caused by a lower or higher staining intensity of the peptidergic fibers, whereas no changes were found in the cell bodies. | 1987 | 2440523 |
| [enhancement of the therapeutic efficacy of blood substitutes in burn shock]. | | 1987 | 2440759 |
| a new set of monoclonal antibodies to human mhc class ii alpha chains demonstrates that most alpha epitopes are inaccessible on the living cell surface. | when mice were immunized with a mixture of human mhc class ii alpha and beta glycoprotein chains, the predominant antibody response was anti-alpha, and from a subsequent fusion experiment over 60 hybridomas showing anti-alpha activity were generated, compared with 11 anti-beta secretors. these findings contrast with the relative paucity of anti-alpha monoclonals described previously. use of a miniaturized western blot screening protocol was a critical factor in the present study since the anti-a ... | 1987 | 2440796 |
| a new set of monoclonal antibodies to human mhc class ii alpha chains demonstrates that most alpha epitopes are inaccessible on the living cell surface. | when mice were immunized with a mixture of human mhc class ii alpha and beta glycoprotein chains, the predominant antibody response was anti-alpha, and from a subsequent fusion experiment over 60 hybridomas showing anti-alpha activity were generated, compared with 11 anti-beta secretors. these findings contrast with the relative paucity of anti-alpha monoclonals described previously. use of a miniaturized western blot screening protocol was a critical factor in the present study since the anti-a ... | 1987 | 2440796 |
| [determination of serum antibody avidity compared to infectious o-antigenemia in paratyphoid b patients]. | the avidity of serum antibodies depending on infectious o-antigenemia in patients with paratyphoid b has been studied in the course of the disease. the study has revealed a faintly pronounced inverse correlation between the degree of avidity of serum antibodies and the level of infectious antigenemia. the avidity of serum antibodies in paratyphoid b has been found to increase in the course of the disease. a significant dependence of the severity of the disease on the avidity of serum antibodies ... | 1987 | 2441547 |
| [nonspecific and antigen-specific adhesiveness of circulating lymphocytes in dysentery patients undergoing combined therapy using thymalin and indomethacin]. | nonspecific adhesiveness of lymphocytes to polyacrylonitrile fiber and the antigen-specific adhesiveness of t-lymphocytes to shigella flexneri pellicular immunosorbent have been studied in dysentery patients. short courses of treatment with indomethacin, but not with thymalin (the preparation of thymic hormones), used for immunomodulation, have accelerated the normalization of nonspecific lymphocyte adhesiveness during regression of the disease, while indomethacin, in contrast to thymalin, inhib ... | 1987 | 2441548 |
| discrimination of hepatocyte-stimulating activity from human recombinant tumor necrosis factor alpha. | the involvement of tumor necrosis factor alpha (tnf alpha) in the regulation of acute-phase protein synthesis is currently under discussion. in this study the effect of human recombinant tnf alpha on the regulation of the 4 acute-phase proteins alpha 2-macroglobulin, albumin, alpha 1-proteinase inhibitor and alpha 1-acute-phase globulin was investigated in rat hepatocyte primary cultures. no changes in synthesis of any of the 4 proteins were observed. however, an acute-phase response similar to ... | 1987 | 2441999 |
| influence of neuropeptides on airway smooth muscle. | the first bronchoactive (and vasoactive) peptide to be discovered in the lung was isolated and characterized in 1970 from an embryologically related organ, the small intestine. since then, more than 20 additional peptides have been described in lung tissue and their biologic activities investigated. many of these are neuropeptides acting as neurotransmitters or neuromodulators to influence airway, pulmonary vascular, and other functions. more neuropeptides are known to exist in the brain and per ... | 1987 | 2446543 |
| acetylcholine receptor-specific human t-lymphocyte lines. | | 1987 | 2446551 |
| definition of myasthenogenic sites of the human acetylcholine receptor using synthetic peptides. | experimental autoimmune myasthenia gravis (eamg) and antibodies that modulate achrs from cultured human muscle are induced by a disulfide-looped peptide comprising the human acetylcholine receptor (achr) alpha-subunit residues 125-147 (h alpha 125-147). to delineate the essential antigenic requirements for induction of eamg by this peptide, a series of peptides was synthesized: (a) a nonlooped analog (cys 128 replaced by ser) stimulated modulating autoantibodies, induced eamg, and bound antibodi ... | 1987 | 2446556 |
| distributions of certain neuropeptides in the primate thalamus. | the distributions of fibers and terminals immunoreactive for somatostatin (srif), neuropeptide y (npy), substance p (sp) and cholecystokinin octapeptide (cck), were studied in the diencephalon of cynomolgus monkeys. immunoreactivity for all 4 peptides is found in extrinsic afferent fibers innervating the dorsal thalamus, ventral thalamus and epithalamus. the distributions of such fibers are more extensive than previously described and include many relay nuclei in their zones of terminations. sp ... | 1987 | 2446708 |
| heterogeneity in chromosome damage and repair rates after bleomycin in ataxia telangiectasia cells. | cells derived from patients with ataxia telangiectasia (at) are known to be exceptionally sensitive to ionizing radiation and chemotherapeutic agents such as bleomycin (blm), neocarzinostatin, and etoposide. this increased sensitivity is manifested by high chromosome aberration frequencies after treatment. in order to probe the underlying basis for this phenomenon, the technique of premature chromosome condensation was used to determine whether the increased chromosome damage observed after bleo ... | 1988 | 2446745 |
| antiviral chemotherapy and prophylaxis of viral respiratory disease. | respiratory viruses continue to be major causes of morbidity and mortality. currently available chemotherapy is limited to oral amantadine for uncomplicated influenza a and aerosolized ribavirin for respiratory syncytial virus (rsv) infections. amantadine is also efficacious for chemoprophylaxis of influenza a virus infections. rimantadine has similar clinical efficacy and is better tolerated than amantadine. aerosolized ribavirin may be useful in the treatment of serious respiratory illness cau ... | 1987 | 2446820 |
| theophylline's inhibition of antigen-induced contraction of human parenchymal strips is independent of adenosine antagonism. | the effects of theophylline, and adenosine antagonist, and enprofylline, reported to lack adenosine antagonism, were studied using antigen-induced contraction of sensitized human lung parenchymal strips. cumulative addition of adenosine usually resulted in a small concentration-dependent contraction, which was antagonized by theophylline, 100 microm. antigen-induced contraction was reduced by pretreatment with adenosine, 10 microm, but not by adenosine addition after antigen challenge. dipyridam ... | 1987 | 2446888 |
| inhibition of allergic encephalomyelitis by the iron chelating agent desferrioxamine: differential effect depending on type of sensitizing encephalitogen. | induction of experimental allergic encephalomyelitis (eae) in lewis rats by injection of guinea pig (gp) spinal cord homogenate (sch) plus adjuvant (sch-cfa) can be inhibited by treatment with the iron chelating agent desferrioxamine (dfom). interestingly, induction of eae with purified myelin basic protein (bp-cfa) is not inhibited with dfom. this dichotomy does not appear to be due to any quantitative differences in the two inocula since minimal clinical eae produced by threshold levels of bp ... | 1988 | 2447124 |
| structural homology of human complement component c8 gamma and plasma protein hc: identity of the cysteine bond pattern. | anti-c8 alpha-gamma specific antibodies were used to isolate cdna clones from a human liver expression library. antibodies affinity-purified on the expressed hybrid protein of one clone bound exclusively to the gamma-chain of reduced c8 alpha-gamma. this clone, as well as a second full length cdna clone obtained by hybridization screening, were sequenced and the complete primary structure for c8 gamma was established. cyanogen bromide cleavage of c8 alpha-gamma released a 12 kda carboxy-terminal ... | 1987 | 2447883 |
| shiga toxin inhibits the anti-invasive effect of interferons. | | 1988 | 2450154 |
| [relation of the immunosuppressivity of virulent shigella to the structure of o antigen]. | the capacity of filtrates of virulent shigella cultures (vsc) and their different fractions to render avirulent strains, injected intraperitoneally into mice simultaneously with these filtrates, capable of suppressing immune response (delayed hypersensitivity) has been studied. among the fractions of vsc filtrates, the lipoid fraction soluble in the mixture of ethanol and ether has proved to be active. active factors can be extracted from vsc filtrates by means of immunosorbents obtained from an ... | 1988 | 2452538 |
| high-yield purification of a pp60c-src related protein-tyrosine kinase from human platelets. | a protein-tyrosine kinase (ptk, ec 2.7.1.112) from human platelets was purified with high yield. purification of the enzyme involved sequential chromatography on casein-agarose, tyrosine-agarose, heparin-sepharose and hydroxylapatite. the procedure resulted in substantially enriched 54/52 kda polypeptides on sds-polyacrylamide gel electrophoresis and a yield of about 25% in ptk activity. about 250 micrograms of purified protein could be obtained from 1 g of cell protein. the purification factor ... | 1988 | 2453218 |
| natural killer cell activation and interferon production by peripheral blood lymphocytes after exposure to bacteria. | we have previously shown that peripheral blood natural killer (nk) cells have significant levels of cytotoxic activity against shigella flexneri-infected hela cells. in this report, we show that nk cell activity against k562 tumor cells and shigella flexneri-infected hela cells can be greatly enhanced by preincubating peripheral blood lymphocytes (pbl) for 18 h with kanamycin-treated shigella flexneri or salmonella typhimurium. cell-free supernatants obtained from pbl-bacteria cultures contained ... | 1988 | 2453465 |
| activation of the mas oncogene during transfection of monoblastic cell line dna. | using the tumorigenicity assay, we observed an activation of the mas oncogene during transfection of human acute myelocytic leukemia (ctv-2) dna. respective transfectants contained amplified mas sequences characterized by rearrangements in 5' and 3' noncoding regions that were transcribed into a 3.3 kb mas rna species. our data represent the second report on activation of the mas gene. | 1988 | 2453759 |
| structural and immunochemical studies of the lipopolysaccharide from a new provisional serotype of shigella flexneri. | the chemical structure of the o-antigen of a proposed new provisional serotype of shigella flexneri has been determined. methylation analysis, glc-ms, 1h-nmr and 13c-nmr showed that the linear o-antigenic polysaccharide is the same as for all s. flexneri [kenne, l., lindberg, b., petersson, k. & romanowska, e. (1977) carbohydr. res. 56, 363-370]. a novel structural feature is that the disaccharide alpha-d-glcp-(1----2)-alpha-d-glcp is linked to o4 of the n-acetyl-glucosamine residue. (formula: s ... | 1988 | 2458260 |
| shigella flexneri invasion plasmid antigens b and c: epitope location and characterization with monoclonal antibodies. | invasion plasmid antigens b (ipab) and c (ipac) are associated with the ability of shigellae to invade cultured mammalian cells. monoclonal antibodies against ipab and ipac polypeptides were produced and used in a whole-cell enzyme-linked immunosorbent assay to show that both ipab and ipac polypeptides were exposed on the surface of virulent shigellae. moreover, these surface epitopes were shown to be highly conserved among different serotypes of shigella spp. and enteroinvasive escherichia coli ... | 1988 | 2459066 |
| bacterial epitopes involved in the induction of reactive arthritis. | a panel of documented arthritogenic shigella flexneri strains as well as an epidemic-associated non-arthritogenic shigella sonnei control was used to identify a 2-megadalton plasmid specific to the arthritogenic strains. the plasmid, phs-2, contains a dna sequence that encodes a 22-amino acid polypeptide encompassing a pentapeptide homologous to part of the polymorphic region of the alpha-1 domain of hla-b27. these results suggest that molecular mimicry between arthritogenic bacterial-encoded ep ... | 1988 | 2462352 |
| immunoglobulin m, a, and g antibody response to lipopolysaccharide o antigen in symptomatic and asymptomatic shigella infections. | the antilipopolysaccharide antibody response in sera obtained from subjects involved in 10 outbreaks of shigellosis occurring in israeli military field units was determined by an enzyme-linked immunosorbent assay and a passive hemagglutination test. both tests were found to be sensitive and specific for the diagnosis of shigellosis. a significant antibody response was detected in 73 to 82% of the symptomatic and 48 to 60% of the asymptomatic subjects during the shigella sonnei and shigella flexn ... | 1989 | 2463995 |
| [specificity of the protective action of a ribosomal shigella vaccine and the absence of activity in the ribosomes from r mutants]. | the ribosomal preparations of s. sonnei and some other bacterial species were obtained by the method of differential centrifugation, and the specificity of their protective action was studied in the keratoconjunctivitis test on guinea pigs. the ribosomal preparations were introduced parenterally in a single injection, and their protective action was determined two weeks later by the challenge of the animals with s. sonnei virulent strain and the subsequent calculation of the efficiency index (ei ... | 1988 | 2464261 |
| [lipid peroxidation in experimental viral infection and the effect on it of interferons and alpha-tocopherol]. | lipid peroxidation as well as effects of various interferons (native, from fibroblasts of new born and adult animals) on the peroxidation were studied in blood plasma, erythrocytes, liver and kidney tissues of mice with viral infection. these interferons, simultaneously with antiviral action, exhibited the positive effect on lipid peroxidation, while the interferon from fibroblasts of newborns produced the side reactions. simultaneous use of the interferon and alpha-tocopherol removed these side ... | 1988 | 2467439 |
| [comparative diagnostic value of methods for detecting dysentery antigens in substrates of the patient's body]. | the comparative evaluation of different immunological methods, such as the enzyme immunoassay, the aggregate hemagglutination test and the complement fixation test, used for the detection of specific shigella antigens in biological body substrates obtained from 287 patients with acute dysentery caused by s. sonnei, s. flexneri and s. newcastle has been carried out. the enzyme immunoassay and the aggregate hemagglutination test most effective (97.5 +/- 0.5 and 92.4 +/- 0.9, respectively), the obj ... | 1989 | 2469267 |
| feeding by mucin and intestinal growth of some enteric bacterial pathogens. | enteroinvasive escherichia coli, salmonella typhi-murium, shigella sonnei, shigella flexneri, as well as e. coli k-12 show dose dependent growth in minimal medium completed with purified hog gastric "granular mucin". this ability is based on alpha-galactosidase production: defective, melibiose (and galactose) non-fermenting k-12 mutant were unable to utilize mucin. the viability of the parent k-12 strain in the cecal content of mice is significantly higher than that of its mel- mutant phenotype. ... | 1988 | 2469280 |
| human milk immunoglobulin a antibodies to shigella virulence determinants. | because human milk is thought to protect infants from shigellosis, we evaluated milk for immunoglobulin a to shigella virulence determinants. milk was preincubated to remove antibodies unrelated to each locus of interest, using defined shigella and e. coli hybrids containing known shigella genetic segments prior to immunoblotting. the milk could not be shown to contain antibodies to chromosomally encoded virulence loci except for the expected antibodies to the products of the histidine locus. ho ... | 1989 | 2470676 |
| in vitro treatment of hep-2 cells with human tumor necrosis factor-alpha and human interferons reduces invasiveness of salmonella typhimurium. | enteroinvasive bacteria, like salmonella typhimurium, can be internalized in in vitro cultured epithelioidal cells, like hep-2 cells. this phenomenon is inhibited by pretreatment of cells with human tumor necrosis factor alpha (tnf-alpha) in a dose- and time-dependent manner. the effect was also reproduced in other cell types, including diploid embryo fibroblast cells. the tnf-alpha effect was neutralized by anti-tnf-alpha antibodies. no synergistic effect was produced by combinations of tnf-alp ... | 1989 | 2472042 |
| expression in escherichia coli k-12 of the 76,000-dalton iron-regulated outer membrane protein of shigella flexneri confers sensitivity to cloacin df13 in the absence of shigella o antigen. | one of the chromosomal segments associated with virulence in shigella flexneri encodes the production of aerobactin and the synthesis of an iron-regulated 76-kilodalton outer membrane protein believed to be the ferric-aerobactin receptor. however, s. flexneri expressing this putative aerobactin receptor, which is slightly larger than that encoded by pcolv, is insensitive to the killing action of cloacin df13, a bacteriocin which binds to other aerobactin receptor proteins and kills the cells. in ... | 1989 | 2474501 |
| a dual transcriptional activation system for the 230 kb plasmid genes coding for virulence-associated antigens of shigella flexneri. | the expression of plasmid-encoded, invasion-related antigens lpa b, c and d of shigella flexneri was found to be positively regulated at transcriptional level by a 33kd protein produced by the previously defined, virulence-associated region 1 on the sali fragment b of the 230 kb invasion plasmid. the gene (designated virb) was identified and its nucleotide sequence determined. no ipa b or c was produced in the absence of an intact virb gene although lower levels of d were produced. the previousl ... | 1989 | 2474742 |
| identification of a 2-md plasmid from shigella flexneri associated with reactive arthritis. | the development of reactive arthritis, a sterile inflammatory polyarthropathy that primarily affects hla-b27 positive individuals, has been associated with previous enteric infections caused by various gram-negative bacteria. the possibility that a common bacterial epitope triggers the disease was investigated by screening a panel of documented arthritogenic shigella strains as well as 2 epidemic-associated nonarthritogenic shigella controls. a 2-md plasmid specific to the arthritogenic strains ... | 1989 | 2475114 |
| identification and characterization of transcription termination sites in the escherichia coli lacz gene. | the escherichia coli lacz gene contains a series of latent transcriptional terminators that are responsible for the polar effects of certain mutations. we demonstrate, using gel electrophoretic size analyses and nuclease s1 mapping procedures, that rna polymerase terminates rna synthesis in the vicinity of five positions 180, 220, 379, 421 and 463 base-pairs downstream from the start point during transcription of lacz dna in vitro in the presence of rho factor. termination at all but the 421 pos ... | 1989 | 2475637 |
| antihypertensive, anticalcinotic, and antiarteriosclerotic properties of anipamil, a long-acting new derivative of verapamil. | the classical indication for calcium antagonists has for a long time been coronary heart disease. however, more recent experimental data as well as a host of clinical observations now encourage a broader use of these drugs also for antihypertensive purposes and for prevention of concomitant sclerotic vascular injury. needless to say, for the treatment of hypertensive crises rapidly acting calcium antagonists are required, whereas for chronic therapy of hypertension calcium antagonists with a lon ... | 1989 | 2475679 |
| [development and use of solid-phase test-systems for the detection of o-antigen of shigella sonnei and shigella flexneri]. | the possibility of the diagnosis of dysentery caused by s. sonnei and s. flexneri, as well as the determination of the dynamics of the distribution of specific o-antigen in the patient's body, by means of the enzyme-linked immunosorbent assay system developed on the basis of antibody preparations obtained by immunosorption has been studied. the study has shown that for better diagnosis the use of fecal extracts is preferable in assays; when used in combination with bacteriological analysis, thes ... | 1989 | 2477970 |
| [use of immunoenzyme analysis in the rapid diagnosis and study of the characteristics of circulating o-antigen in biological fluids of patients with acute dysentery sonne]. | the data on the clinical approval of the original enzyme immunoassay system for the determination of somatic o-antigen in the blood serum and urine of patients with acute sonne dysentery are presented. the level of the antigen determined in the biological fluids of patients has been shown to depend on the severity of the disease. different types of dynamic curves, reflecting the level of o-antigen in the biological fluids of patients with acute sonne dysentery and characteristic of different cli ... | 1989 | 2477971 |
| [cloning of the gene relative to invasive ability of shigella flexneri 5]. | a genomic library of s. flexneri 5 large plasmid (140 md) was constructed using the cosmid pjb8 as vector. there were more than 4,000 transformants in the library. 66 clones were picked out from the library by hybridization with 17kb gene probe relative to invasive ability. a few clones were analyzed. the results indicated that all these clones tested contained recombinant plasmids, they could hybridize with 17 kb gene probe. when these recombinant plasmids were digested with ecor1, 17 kb fragme ... | 1989 | 2486252 |
| mechanism of antigenic variation in shigella flexneri bacilli. i. selective lethal effect of normal sera on mixed population of s. flexneri 1b serotype and its antigenic 3b mutant. | normal non-immune calf, human and mice sera kill-preferentially the shigella flexneri 1b serotype cells with antigenic formula i; 3, 4, 6 leaving the antigenic mutant cells of the serotype 3b with antigenic formula iii; 3, 4, 6. the strongest lethal action was observed in normal calf serum. this serum makes possible to obtain the 3b serotypes mutant from pure population of 1b serotype cells. thermal inactivation of the complement deprives the serum of its selective activity. | 1989 | 2487379 |
| lack of effect by prostaglandin f2 alpha and verapamil on calcium uptake by isolated corpora lutea from pseudopregnant rats. | | 1989 | 2492809 |
| intracisternal injection of a trh analogue stimulates gastric luminal serotonin release in rats. | intracisternal injection of trh analogue, rx 77368, which has been previously shown to induce a vagal-dependent stimulation of gastric acid secretion and contractility dose-dependently (10-300 ng) stimulated serotonin release into the gastric lumen of conscious pylorus-ligated rats. the stimulation of gastric acid and serotonin release occurred 15 and 30 min, respectively, after intracisternal injection of rx 77368 (100 ng) and lasted for over 2 h, as measured in urethan-anesthetized rats with g ... | 1989 | 2493198 |
| renal function and morphology in sudanese patients with advanced hepatosplenic schistosomiasis and portal hypertension. | the association between glomerular disease and hepatosplenic schistosomiasis is well documented in reports from south america. during the present hospital investigation in sudan, 58 patients admitted for intercurrent complications of advanced hepatosplenic schistosomiasis were studied. the patients, median age 35 years, had no concurrent schistosoma haematobium infection. diagnostic criteria included an enlarged spleen (n = 58), at least 1 episode of hematemesis (n = 55) and/or melena (n = 36), ... | 1989 | 2493202 |
| in vitro susceptibility of feline herpesvirus-1 to vidarabine, idoxuridine, trifluridine, acyclovir, or bromovinyldeoxyuridine. | in vitro activities of 9-[( 2-hydroxyethoxy] methyl) guanine (acyclovir), (e)-5-(2-bromovinyl)-2'deoxyuridine, 9-beta-d-arabinofuranosyladenine (vidarabine), 5-iodo-2'-deoxyuridine (idoxuridine), and 5-trifluoromethyl-2'-deoxyuridine (trifluridine) were studied against 6 strains of feline herpesvirus-1. a significant difference was not detected among viral strains in their susceptibility to these compounds (p = 0.442). the relative potency of these compounds was trifluridine much greater than id ... | 1989 | 2493210 |
| [value of determining the spectrum of blood serum fatty acids in evaluating increasing the effectiveness of antibiotic therapy of dysentery in children with prodigiozan and ephedrine]. | one hundred and ninety one children with acute sonne and flexner dysentery were observed with respect to the disease process, immunity indices and blood serum fatty acid spectrum. 104 children were treated with monomycin alone and 87 children were treated with the antibiotic in combination with prodigiozan and ephedrine as immunostimulators. it was shown that the recovery terms in the patients treated with the use of the immunostimulators decreased as compared to the patients treated with the an ... | 1989 | 2502081 |
| hemagglutinating properties of shigella dysenteriae type 1 and other shigella species. | strains of shigella dysenteriae type 1 cultured in casamino acids-yeast extract broth medium in the presence of 1 mm calcium chloride at 37 degrees c for 22 h induced hemagglutination of erythrocytes that was inhibited by n-acetylneuraminic acid, n-acetylneuramin-lactose, and alpha 1-glycoprotein. the hemagglutination was heat labile, and the absence of cell-surface appendages suggested a nonfimbrial adhesin(s). under the same conditions, strains of shigella flexneri (types 1a, 1b, 2a, and 2b) s ... | 1989 | 2503449 |
| infections in british clinical laboratories, 1986-87. | during 1986-87 this continuing survey showed 15 specific infections in the staff of 235 laboratories, representing 28,524 person years of exposure. the community was the probable source of four of the five cases of tuberculosis and one of the five cases of salmonellosis. occupational exposure was the probable cause of four infections by shigella flexneri, three by salmonella typhimurium, and one by s typhi, all affecting medical laboratory scientific officers (mlsos) in microbiology. occupationa ... | 1989 | 2503546 |
| [clinical studies on patients with legal communicable diseases encountered from 1981 to 1986]. | patients with legal communicable diseases admitted to kawasaki municipal hospital between 1981 and 1986 were studied. ages of patients were over 15 year-old. during this period 115 patients were admitted. out of these patients 84 had shigellosis, and 9 had amebiasis. out of 20 salmonellosis, 18 were due to salmonella typhi and 2 were due to salmonella paratyphi-a, respectively. two were admitted because of cholera. out of the patients with shigellosis, 27 were domestic and 57 were foreign infect ... | 1989 | 2504839 |
| [the isolation and immunologic study of ribosomal preparations from shigella flexneri]. | s. flexneri ribosomal preparations were isolated by differential centrifugation or by fractionation with polyethylene glycol-6000. their chemical composition and spectrophotometric properties were characteristic of ribosomes, and, as shown by the results of the serological assay, the content of o-specific component was, on the average, 1.4%. the ribosomal preparations were nontoxic for mice when injected intraperitoneally and intravenously in large doses and induced systemic o-antibody response ... | 1989 | 2511706 |
| [in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics]. | in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ... | 1989 | 2518646 |
| [in vitro cultivation of human pulpal fibroblast strains--permanent and deciduous teeth]. | we succeeded in separating and the cultivating stable monolayer cultures of dental pulp fibroblast strains derived from permanent and deciduous human teeth. human permanent (n = 67) and deciduous teeth (n = 26) were extracted under acupuncture anaesthesia for the correction of malocclusion. after splitting the teeth, the pulp tissues were carefully removed, placed in tissue culture flasks, and grown in dulbecco's modified eagle medium supplemented with 10% fetal calf serum (fcs). the human pulpa ... | 1989 | 2519279 |
| [uropathogenic serratia marcescens of hospital origin: analysis of the adhesion mechanism of 2 strains]. | we isolated 2 serratia marcescens strains, fim (0) and fim (-), from patients with urinary tract infection. adhesion properties were studied by hemagglutination, response of uroepithelial cells and electron microscopy techniques. s marcescens fim (+) showed high adherence to uroepithelial cells, hemagglutination capacity and peculiar electron microscopy characteristics. all of these are absent in the fim (-) variety. the fim (+) strain was resistant to nitrofurantoin, nalidixic acid, tetracyclin ... | 1989 | 2519411 |
| bidimensional reversed-phase high-pressure liquid chromatography analysis of cultured cell neuropeptides: application to atrial natriuretic factor. | we report here a one-step procedure for extraction and analysis of neuropeptides in chromaffin cell culture media and acid extracts using reversed-phase hplc. the bidimensional hplc system consists of a precolumn connected to a six-port switching valve which is on-line with an analytical column. the direct injection of the biological samples onto the precolumn previously equilibrated with 15% acetonitrile allows the elimination of interfering substances. the samples purified on the precolumn can ... | 1989 | 2527010 |
| determination of the secondary structure of the dna binding protein ner from phage mu using 1h homonuclear and 15n-1h heteronuclear nmr spectroscopy. | the sequential resonance assignment of the 1h and 15n nmr spectra of the dna binding protein ner from phage mu is presented. this is carried out by using a combination of 1h-1h and 1h-15n two-dimensional experiments. the availability of completely labeled 15n protein enabled us to record a variety of relayed heteronuclear multiple quantum coherence experiments, thereby enabling the correlation of proton-proton through-space and through-bond connectivities with the chemical shift of the directly ... | 1989 | 2527559 |
| immunogenicity of a yeast-derived hepatitis b vaccine in hemodialysis patients. | in a multicenter study of hemodialysis patients in spain, the immunogenicity of a yeast-derived recombinant deoxyribonucleic acid hepatitis b vaccine was evaluated. two different vaccination schedules were examined: zero, one, two, six months and zero, one, two, 12 months. two different dose levels (20 micrograms and 40 micrograms) were also compared. no serious adverse effects were reported by any of the vaccinees; the most frequently reported reaction was soreness at the injection site. this s ... | 1989 | 2528295 |
| 1-(3-trifluoromethylphenyl) piperazine (tfmpp) in the ventral tegmental area reduces the effect of desipramine in the forced swimming test in rats: possible role of serotonin receptors. | 1-(3-trifluoromethylphenyl)piperazine (tfmpp), a serotonin1 (5-ht1) receptor agonist, injected i.p. in doses of 0.1 and 0.6 mg/kg, did not modify the immobility time of rats in the forced swimming test but significantly antagonized the effect of a 7 days treatment with 10 mg/kg per day desipramine (dmi). a similar effect was found on infusing 1 and 5 micrograms/microliters tfmpp bilaterally into the ventral tegmental area (vta). infusion of 5 micrograms/microliters tfmpp into the nucleus accumbe ... | 1989 | 2533079 |
| identification of icsa, a plasmid locus of shigella flexneri that governs bacterial intra- and intercellular spread through interaction with f-actin. | the capacity of shigella to spread within the cytosol of infected epithelial cells and to infect adjacent cells is critical for the development of infection foci, which lead to mucosal abscesses. shigella is a nonmotile microorganism that appears to utilize host cell microfilaments to generate intra- as well as intercellular movements, since this movement was inhibited by cytochalasin d and involvement of f-actin was demonstrated by direct labeling of infected cells with the specific dye n-(7-ni ... | 1989 | 2542950 |
| human endothelial cells inhibit platelet aggregation by separately stimulating platelet cyclic amp and cyclic gmp. | thrombin-induced platelet aggregation was monitored in a mixture of washed human platelets and cultured human endothelial cells on microcarrier beads. endothelial cells completely inhibited platelet aggregation and enhanced the content of both cyclic amp and cyclic gmp in the platelets. inhibition of endothelial prostacyclin synthesis with indomethacin abolished the cyclic amp increase in the platelets, whereas the cyclic gmp increase was unimpaired. a significant component of the endothelial an ... | 1989 | 2546782 |
| characterization of cdna clones encoding two putative isoforms of the alpha 1 subunit of the dihydropyridine-sensitive voltage-dependent calcium channel isolated from rat brain and rat aorta. | cdna clones encoding rat brain and rat aorta isoforms of the alpha 1 subunit of the dihydropyridine-sensitive, voltage-dependent calcium channel were isolated and sequenced. these tissue-specific cdna clones share significant amino acid similarity with the rabbit skeletal muscle calcium channel alpha 1 subunit (75% and 66% amino acid identity for rat brain and rat aorta isoforms, respectively). northern analysis revealed transcript sizes of 6.5 and 8.6 kb in aorta and 8.6 kb in brain. | 1989 | 2546805 |
| influence of non-esterified fatty acids on respiratory control of reconstituted cytochrome-c oxidase. | bovine heart cytochrome-c oxidase was reconstituted in liposomes (asolectin) and the activity measured in the presence and absence of uncoupler at increasing concentrations of non-esterified fatty acids. palmitic and stearic acids resulted in a decrease of about 40% in the respiratory control ratio at a concentration of 1 microm, when measured using a spectrophotometric procedure but not with a polarographic assay method. at higher fatty acid concentrations no further change was found. a 50% dec ... | 1989 | 2546826 |
| isolation and characterisation of calcineurin from adrenal cell cytoskeleton: identification of substrates for ca2+-calmodulin-dependent phosphatase activity. | ca2+-calmodulin-dependent protein phosphatase activity is found in cytoskeletons of y-1 mouse adrenal and bovine fasciculata cells. the activity is inhibited by three inhibitors of calmodulin (trifluoperazine, w-7 and pimozide) with ec50 in the low micromolar range. protein phosphatase activity is inhibited by vanadate, fluoride, zn2+ and pyrophosphate, stimulated by mn2+ and found to be tightly bound to the cytoskeleton. substrates for endogenous phosphatase activity were defined by one- and tw ... | 1989 | 2546840 |
| hypoglycaemia induced by antibodies to insulin receptor following a bone marrow transplantation in an immunodeficient child. | severe hypoglycaemia developed seven months after a bone marrow transplantation in a child with severe combined immunodeficiency. his serum exerted potent insulin-like activity: (a) it stimulated insulin receptor autophosphorylation and kinase activity in cell-free systems, this effect being additive to insulin; (b) it increased glucose transport in isolated soleus muscle. these insulin-like effects were due to immunoglobulins against the insulin receptor. indeed, the patient serum immunoprecipi ... | 1989 | 2546845 |
| human papillomavirus and cervical cancer. summary report. | | 1989 | 2546883 |
| functional organization and nucleotide sequence of virulence region-2 on the large virulence plasmid in shigella flexneri 2a. | the 7 kb virulence region-2 of the large (virulence) plasmid in shigella flexneri 2a encodes several proteins required for invasion of intestinal epithelial cells. insertion and deletion mutagenesis, dna subcloning and sds-polyacrylamide gel electrophoresis of proteins synthesized in minicells demonstrated five genes in this region. they encode 24, 18, 62 (ipab), 41 (ipac) and 37 (ipad)-kilodalton (kd) proteins. complementation of tn5-induced mutations in region-2 with the above plasmid construc ... | 1989 | 2552264 |
| thyrotropic activity of the ovine corticotropin-releasing factor in the chick embryo. | an injection of several doses of ovine corticotropin-releasing factor (ocrf) was made into an allantoic blood vessel of 18-day-old chick embryos. all doses used (0.5, 1, 2, and 5 micrograms) induce a quick increase of plasma corticosterone concentrations after 15 and 30 min and 1 and 2 hr and this in a dose-dependent manner with the high doses having a prolonged effect lasting up to 2 hr. an increase in plasma levels of thyroxine (t4) and triiodothyronine (t3) was observed after 1 and 2 hr. afte ... | 1989 | 2555247 |
| autotransplantation of avian parathyroid glands: an animal model for studying parathyroid function. | the parathyroid glands of chickens were autotransplanted and the return of parathyroid function following transplantation was determined. parathyroidectomy (ptx) resulted in a marked hypocalcemia (5.2 +/- 0.2 mg/dl) 4 hr following ptx. plasma calcium (pca) had declined to 4.3 +/- 0.2 mg/dl 24 hr after ptx. parathyroid glands were transplanted subcutaneously 24 hr after removal and 24 hr later, pca had risen to 8.6 +/- 0.5 mg/dl. seven days after ptx. pca increased to 10.3 +/- 0.2 mg/dl and by 14 ... | 1989 | 2555250 |
| [assessment of diagnostic prospects for a method of immunoenzyme analysis during mass testing of feces of patients for the purpose of detecting rotavirus antigens]. | | 1989 | 2555970 |
| enzyme polymorphism and clinical variability of diseases: a study of diabetes mellitus. | we investigated possible relations among four common neonatal manifestations of diabetic pregnancy (macrosomia, hypoglycemia, hypocalcemia, jaundice) and four enzyme polymorphisms (pgm1, ada, ak1, acp1 in a sample of infants born of diabetic mothers. the pattern of associations observed between the two sets of variables is consistent with known differences in enzymatic activity within phenotypes of each system, suggesting that low enzymatic activity may have unfavorable effects on fetal developm ... | 1989 | 2556341 |
| in vivo presynaptic modulation of serotonergic neurotransmission in the rat hippocampus by diazepam. | the effect of the benzodiazepine agonist, diazepam, on serotonergic (5-ht) neurotransmission was assessed in in vivo electrophysiological experiments. diazepam enhanced, in a dose-dependent manner (0.05-2 mg/kg i.v.), the effect of electrical stimulation of the ascending 5-ht pathway on the firing activity of dorsal hippocampus pyramidal neurons. this effect was blocked by the benzodiazepine antagonist, flumazenil. diazepam did not modify the efficacy of microiontophoretic application of 5-ht an ... | 1989 | 2559857 |
| [acute maternal anterior poliomyelitis in a non-endemic zone]. | the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus. | 1989 | 2561040 |
| characterization of l-glutamate action on the release of endogenous dopamine from the rat caudate-putamen. | in the present study the effect of l-glutamic acid (l-glu), n-methyl-d-aspartic acid (nmda), kainic acid (ka) and quisqualic acid (quis) on the release of endogenous dopamine (da) from slices of the rat caudate-putamen was investigated. da was measured by high-performance liquid chromatography coupled to an electrochemical detector. l-glu, nmda, ka and quis, in the absence of mg++, produced a dose-related, ca++-dependent increase in da release. the order of agonist efficacy was l-glu greater tha ... | 1989 | 2563769 |
| effect of endogenous opioid peptides on trh release from rat stomach in vitro. | the effect of endogenous opioid peptides (beta-endorphin, leucine-enkephalin, dynorphin 1-13 on the release of thyrotropin releasing hormone (trh) from the rat stomach in vitro was studied. the rat stomach was incubated in the medium 199 with 1.0 mg/ml of bacitracin (ph 7.4) (medium) and the amount of trh released into the medium was measured by radioimmunoassay. the release of trh from the rat stomach was inhibited significantly in a dose-related manner with the addition of endogenous opioid pe ... | 1989 | 2565806 |
| interleukin-6 in the epidermis of patients with psoriasis before and during puva treatment. | biopsies from lesional and unaffected skin of 6 patients with psoriasis, taken before and during treatment with psoralen plus uva (puva) were examined immunohistologically, using partially purified polyclonal antibodies to crude supernatants of activated human blood monocytes. by absorption with recombinant derived human monokines, we were able to demonstrate that interleukin-6 (il-6) (but not il-1 alpha or il-1 beta) was located in a laminar and granular pattern in stratum corneum, and on epide ... | 1989 | 2566221 |
| genome type analysis of adenoviruses: isolates from one year from the hannover area. | adenoviruses (av), isolated from 138 children during the year 1981 in the hannover area, were studied by dna restriction analysis with the enzymes bamhi, bglii, bsteii, ecori, hindiii, kpni, and smai and compared with the respective prototypes. varying fragment patterns were depicted and genome types analyzed. prototype-like strains of av1 and 5 were not found. types 2, 5, 1, 3, and 7 showed decreasing genetic variation in that order. altered restriction sites were physically mapped on the genom ... | 1989 | 2566302 |
| agonistic effects of anti-cd2 and anti-cd16 antibodies on human natural killer killing. | two monoclonal antibodies (moab), 9-1 (anti-cd2) and 3g8 (anti-cd16), were previously shown to enhance the cytotoxic activity of human natural killer (nk) cells. the present study examined the effect of 9-1 and 3g8 with different effector and target cells to determine whether they activate nk cells through a common mechanism. analysis of purified lymphocyte subpopulations demonstrated that the cd3+cd16+cd3- nk effector cell population is enhanced by both antibodies, while purified cd2+cd16-cd3+ ... | 1989 | 2567053 |