| effect of histamine-sensitizing factor of bordetella pertussis on pharmacologic response of rat atria. | the effects of sensitization with the histamine-sensitizing factor (hsf) of bordetella pertussis as well as bordetella vaccines on a pharmacologic response in rat heart preparations were determined. in normal rats the spontaneous beating of atria in vitro through the positive inotropic action produced by the addition of epinephrine was inhibited immediately by addition of acetylcholine, whereas in the b. pertussis vaccine-treated rats the exciting atria were scarcely inhibited by acetylcholine. ... | 1982 | 6283322 |
| amphiphile-mediated activation of soluble adenylate cyclase of bordetella pertussis. | | 1982 | 6284053 |
| calcium-independent stimulation of bordetella pertussis adenylate cyclase by calmodulin. | bordetella pertussis produces an extracellular adenylate cyclase activity that is present in the culture medium of exponentially growing cells. we have determined that calmodulin (cam) stimulates this enzyme both in the presence and in the absence of free ca2+. in the presence of 90 micron ca2+ half-maximal stimulation of the enzyme occurred at 95 pm calmodulin. comparable levels of calmodulin stimulation were observed when free ca2+ levels were minimized by using egta-containing buffers. howeve ... | 1982 | 6284209 |
| effect of lymphocytosis-promoting factor from bordetella pertussis on cerebellar cyclic gmp levels. | dermonecrotic toxin (dnt), lipopolysaccharide (lps), and lymphocytosis-promoting factor (lpf) were isolated from bordetella pertussis and tested for neuroactivity. when injected intraperitoneally into rats, a dose of 0.13 mg of lpf per kg elevated the cyclic gmp level in cerebellum by approximately 70%, whereas dnt (0.5 mg/kg) and lps (1.5 mg/kg) were without effect. this action of lpf on the central nervous system was dose dependent and did not require the administration of any additional agent ... | 1982 | 6284647 |
| characteristic lipids of bordetella pertussis: simple fatty acid composition, hydroxy fatty acids, and an ornithine-containing lipid. | the lipids and fatty acids of bordetella pertussis (phases i to iv) were analyzed by thin-layer chromatography, gas-liquid chromatography, and mass spectrometry and compared with those of b. parapertussis and b. bronchiseptica. the major lipid components of the three species were phosphatidylethanolamine, cardiolipin, phosphatidylglycerol, lysophosphatidylethanolamine, and an ornithine-containing lipid. the ornithine-containing lipid was characteristic of the genus bordetella. the fatty acid com ... | 1982 | 6284719 |
| [clinical studies on 9, 3"-diacetylmidecamycin in respiratory tract infections in the field of pediatrics (author's transl)]. | laboratory and clinical studies were performed on 9, 3"-diacetylmidecamycin (mom), a new macrolide antibiotic in the field of pediatrics, and the results were as follows. antibacterial activity: for 32 clinically isolated strains of staphylococcus aureus, the mic of mom ranged from 0.78 to 1.56 micrograms/ml for 17 of the 32 strains, and exceeded 100 micrograms/ml for the 15 remaining strains with both inoculum sizes of 10(8) cells/ml and 10(6) cells/ml. for 27 strains of streptococcus pyogenes, ... | 1982 | 6285015 |
| the effect of bordetella pertussis lymphocytosis-promoting factor (lpf) on antibody response in mice: its enhancing and suppressive effects. | the effect of lymphocytosis-promoting factor (lpf) on antibody response in mice was estimated under different sets of experimental conditions. four- and 6-week-old mice were intravenously inoculated with lpf. three days later these mice were inoculated either intraperitoneally or intravenously with sheep red blood cell (srbc) or human serum albumin (hsa) as an antigen. the adjuvant effect of lpf was demonstrated on antibody response in 6-week old mice to intraperitoneally inoculated srbc but not ... | 1982 | 6285047 |
| serological diagnosis of pertussis: igm, iga and igg antibodies against bordetella pertussis measured by enzyme-linked immunosorbent assay (elisa). | igm, iga and igg antibodies against bordetella pertussis were measured by enzyme-linked immunosorbent assay (elisa) with an ultrasonicate of formalin-killed bacteria (a mixture of strains 1, 2 and 1, 2, 3) as antigen and disposable polystyrene 9-cuvette blocks as the solid phase. the specificity properties of the assay were assessed by an inhibition technique. of the microbes tested, only b. parapertussis was able to cause a significant inhibition. in addition, igm and iga antibodies against b. ... | 1982 | 6285449 |
| forskolin stimulation of thyroid adenylate cyclase and cyclic 3',5'-adenosine monophosphate accumulation. | the diterpene forskolin is a potent (100-fold) stimulator of guinea pig thyroid camp accumulation with half-maximal activation occurring at 40 microm. forskolin stimulation is more rapid than that of tsh, attaining a 5-fold increase within 1 min of exposure. the stimulation is also rapidly reversible. the diterpene does not sensitize thyroid camp accumulation to tsh, and the concentration yielding half-maximal response is not altered by the presence of low levels of forskolin. at maximally stimu ... | 1982 | 6286284 |
| effect of bordetella pertussis on alpha 1 and beta-adrenergic and cholinergic muscarinic receptors in guinea pig lung membranes. | after intraperitoneal injection of bordetella pertussis vaccine to guinea pigs, the alpha 1- and beta-adrenergic and cholinergic muscarinic receptors of the whole lung were measured by binding assays with the radioisotope-labeled antagonists, 3h-prazosin, 1-(3)h-dihydroalprenolol and 1-(3)h-quinuclidinyl benzilate, respectively. injection of guinea pigs with pertussis vaccine resulted in an increase in the maximum binding (bmax) of 3h-prazosin, while there was about a 30% reduction in 1-(3)h-dih ... | 1982 | 6286505 |
| repression of adenylate cyclase in the genus bordetella. | virulent phase i strains of bordetella pertussis synthesize the regulatory metabolite adenosine 3',5'-cyclic monophosphate (camp); whereas, non-virulent phase iv strains are unable to do so. these findings were confirmed and extended using additional strains of b. pertussis as well as strains of bordetella parapertussis and bordetella bronchiseptica. levels of adenylate cyclase activity and subsequent camp production were found to correlate. high adenylate cyclase activity during exponential pha ... | 1982 | 6287177 |
| phagocyte impotence caused by an invasive bacterial adenylate cyclase. | for unknown reasons, humans infected with the bacterium bordetella pertussis are exceptionally vulnerable to secondary infections. bordetella species elaborate a soluble, heat-stable, and highly active adenylate cyclase. this enzyme is internalized by phagocytic cells and catalyzes the unregulated formation of adenosine 3',5'-monophosphate (cyclic amp), thereby disrupting normal cellular function. this unusual phenomenon may explain bordetella-induced aphylaxis and may prove to be useful for inv ... | 1982 | 6287574 |
| [isolation and purification of restriction endonuclease bpei from bordetella pertussis]. | new restriction endonuclease has been isolated from bordetella pertussis vaccine strain 305 and purified in 1 stage on sepharose covalently bound with blue dextran. the isolated restrictase has been found capable of breaking down lambda-phag dna into 7 fragments. according to its specificity, bpe i is the isoschizomer of hind iii obtained from haemophilus influenzae strain rd. | 1982 | 6287768 |
| plasmid transfer to bordetella pertussis: conjugation and transformation. | plasmids of the p and w incompatibility groups were introduced into bordetella pertussis by conjugation. plasmid dna isolated from b. pertussis could be reintroduced by transformation. dna isolated from escherichia coli could not be introduced into b. pertussis by transformation if this dna contained hindiii restriction sites. we have demonstrated that hindiii sites are modified by b. pertussis. plasmids of the fi and fii incompatibility groups could not be introduced into b. pertussis by conjug ... | 1982 | 6288667 |
| [study of the dna of the causative agent of whooping cough, bordetella pertussis]. | a number of physico-chemical properties of bordetella pertussis dna has been studied. the values of its floating density and melting point have been established, which has allowed one to calculate the gc composition of b. pertussis dna. the average molecular weights of the fragments of b. pertussis dna, resulting from its hydrolysis with specific endonucleases ecor, bamh 1, sal 1, pst 1, have been determined, and thus the basis has been provided for establishing the number of clones necessary fo ... | 1982 | 6289572 |
| assay of calmodulin with bordetella pertussis adenylate cyclase. | | 1982 | 6289697 |
| stimulatory effect of pertussis toxin on tissue cyclic amp levels in canine thyroid slices. | the in vitro effects of the protein purified from the culture medium of bordetella pertussis (pertussis toxin) on cyclic amp levels in canine thyroid slices were studied. the toxin caused a three-fold increase in tissue cyclic amp levels after 18 h or longer incubation. furthermore, when the slices were incubated with the toxin for 6 h and then incubated without the toxin for further 12 h, similar effects were observed. these results indicate that pertussis toxin elevates tissue cyclic amp level ... | 1982 | 6290206 |
| development of acute autoimmune encephalomyelitis in mice: factors regulating the effector phase of the disease. | the development of acute experimental autoimmune encephalomyelitis (eae) in mice is potentiated by the use of bordetella pertussis vaccine as an adjuvant. histamine sensitizing factor (hsf) extracted from b. pertussis is the active adjuvant agent and causes a mild increase in cerebrovascular permeability. during the development of eae, there is an additional increase in vascular permeability of the brain and spinal cord. the adjuvant action of b. pertussis hsf does not appear to mimic a generali ... | 1982 | 6290379 |
| ornithine-containing lipid of bordetella pertussis that carries hemagglutinating activity. | the proposed structure of the ornithine-containing lipid of bordetella pertussis is 3-hydroxyhexadecanoic acid amide-linked to ornithine and esterified to the second hexadecanoic acid. the aminolipid strongly agglutinates type a and b human erythrocytes. | 1982 | 6290456 |
| effect of pyridines on phenotypic properties of bordetella pertussis. | several conditions of growth of bordetella pertussis cause a reversible phenotypic alteration in properties termed modulation. growth in medium containing nicotinic acid induces normal (x-mode) cells to change to modulated (c-mode) cells. we examined several pyridines and compounds resembling pyridines for their ability to affect modulation, using envelope protein patterns and serological reactivity as indicators of modulation. we found that 6-chloronicotinic acid and quinaldic acid were more ef ... | 1982 | 6292105 |
| purification and characterisation of a fimbrial haemagglutinin from bordetella pertussis for use in an enzyme-linked immunosorbent assay. | the fimbrial haemagglutinin (f-ha) of bordetella pertussis grown on solid medium was extracted with 1m sodium acetate for 72 h at 20 degree c, and partially purified by sephacryl s-300 gel chromatography. a pooled fraction with fimbrial haemmagglutinating activity was shown to contain fimbriae haemagglutinating activity was shown to contain fimbriae of the expected morphology by electron microscopy. chemical and biological assays showed that the f-ha fraction contained some heat-labile agglutino ... | 1982 | 6292428 |
| detection of antibodies in human serum against the fimbrial haemagglutinin of bordetella pertussis by enzyme-linked immunosorbent assay. | antibody responses in human sera against bordetella pertussis during natural infection were investigated by a microplate enzyme-linked immunosorbent assay (elisa) with a purified fimbrial haemagglutinin preparation as antigen. significant rises of specific igg, igm and iga were demonstrated in paired sera. a secondary type of antibody response was found in most children and adults. in children, the type of response correlated with previous vaccination status; there was a primary response in unva ... | 1982 | 6292429 |
| fatty acid analysis for differentiation or bordetella and brucella species. | the fatty acid composition of bordetella pertussis (13 strains), b. parapertussis (3 strains), b. bronchiseptica (6 strains), brucella abortus (6 strains), b. melitensis (4 strains) and b. suis (5 strains) was determined. both genera contained straight-chain saturated and mono-unsaturated acids as well as cyclopropane substituted isomers, but the overall differences between the two genera were distinct. the brucella species contained exclusively c16 to c19 acids. minor amounts of hydroxylated ac ... | 1982 | 6293245 |
| transposon insertion and subsequent donor formation promoted by tn501 in bordetella pertussis. | the mercuric chloride resistance transposon, tn501, was introduced into bordetella pertussis by using the chimeric plasmid puw942, which is unable to replicate in this species. tn501 insertions which conferred a thiamine requirement were the predominant insertion class. in many cases, the mercuric chloride-resistant transconjugants were also resistant to the other plasmid markers, but failure to detect plasmid dna in these isolates indicated that integration of the entire plasmid into the chromo ... | 1983 | 6294051 |
| glycerol-releasing activity of histamine-sensitizing factor of bordetella pertussis for rat adipocytes in vitro. | histamine-sensitizing factor (hsf) purified from bordetella pertussis induced specifically the release of glycerol from rat epididymal adipocytes in vitro. the most sensitive and reproducible results were obtained by using 1 to 2 x 10(5) adipocytes/tube from rats weighing 150 to 200 g, and by incubation at 37 c for 180 min. after a lag period of about 60 min, hsf-treated adipocytes released glycerol in increasing amounts between 60 and 240 min, depending on the dose of hsf. a close correlation b ... | 1982 | 6294484 |
| [changes in the biological properties of the pertussis microbe exposed to antibiotics]. | experimental modeling of the process of variation of the pertussis causative agent grown on the casein-carbon agar medium with subtoxic doses of the widely used antibiotics was performed. significant changes in the morphological, cultural and serological properties of the pertussis microbe, in its sensitivity to the antibiotics and bacteriocins, in the activity of glutamine synthetase and in the electrophoretic mobility of the protein components were shown. the antigen structure determined with ... | 1982 | 6295258 |
| identification of the predominant substrate for adp-ribosylation by islet activating protein. | islet activating protein (iap), a toxin isolated from bordetella pertussis, blocks the ability of inhibitory hormones to attenuate adenylate cyclase activity and enhances the ability of stimulatory hormones to activate the enzyme. the toxin appears to act by catalyzing the transfer of adp ribose from nad to a 41,000-dalton protein in target cell membranes. a protein purified from rabbit liver membranes, apparently composed of 41,000- and 35,000-dalton subunits, is shown to be a specific substrat ... | 1983 | 6296122 |
| inhibition of nicotinic acid and nicotinamide uptake into bordetella pertussis by structural analogues. | 3-pyridine-carboxaldehyde and 3-pyridine-aldoxime were effective and specific inhibitors of the uptake of both nicotinic acid (na) and nicotinamide (nd) by bordetella pertussis, although neither compound inhibited the growth of the bacteria in liquid medium or the oxidation of glutamate by washed suspensions. in contrast, the following pyridine derivatives did not inhibit uptake of na or nd: iso-na, iso-nd, isoniazid, 6-amino-na and 6-amino-nd, 3-acetyl-pyridine, 3-pyridyl-acetic acid, n,n-dieth ... | 1982 | 6296274 |
| macrophage-dependent polyclonal activation of splenocytes by bordetella pertussis endotoxin and its isolated polysaccharide and lipid a regions. | | 1982 | 6297808 |
| cephalexin-supplemented jones-kendrick charcoal agar for selective isolation of bordetella pertussis: comparison with previously described media. | four agar media, jones-kendrick (jk) charcoal agar unsupplemented, jk agar supplemented with 0.5 u of penicillin per ml, jk medium supplemented with 2.5 micrograms of methicillin per ml, and jk medium supplemented with 40 micrograms of cephalexin per ml, were evaluated to determine their ability to support growth of bordetella pertussis, their ability to selectively inhibit normal pharyngeal flora while maintaining growth of b. pertussis, and their stability during storage. five stock cultures o ... | 1983 | 6298274 |
| serologic diagnosis of pertussis: comparison of enzyme-linked immunosorbent assay and bacterial agglutination. | an enzyme-linked immunosorbent assay (elisa) and bacterial agglutination (ba) method for determining the presence of antibodies to bordetella pertussis were compared on serum samples from 21 patients with whooping cough and their 76 family members. the overall diagnostic agreement between the two methods was 77%. the data for ba-detected antibodies correlated best with igg and iga antibodies to b. pertussis. all of the culture-positive patients showed serologic positivity in both assays during t ... | 1983 | 6298315 |
| comparison of biological activities of bordetella pertussis mutants lacking the ability to produce filamentous hemagglutinin with similar activities of other smooth strains. | | 1982 | 6298581 |
| effect of dermonecrotic toxin of bordetella pertussis on the spleen of cfw and c57bl/10scn mice. | | 1982 | 6298582 |
| structure and biological properties of solubilized envelope proteins of bordetella pertussis. | the structure and biological properties of solubilized envelope proteins of bordetella pertussis have been examined. several envelope proteins were found to be specific for phase i strains of b. pertussis and could be isolated by selective detergent extraction. these proteins had molecular weights of 90,000, 86,000, 81,000, 33,000, 31,000, and 30,000 and were reduced or absent in envelope preparations from bordetella bronchiseptica, bordetella parapertussis, or phase iv strains of b. pertussis. ... | 1983 | 6299946 |
| bordetella pertussis isolation in general practice: 1977-79 whooping cough epidemic in west glamorgan. | some of the factors influencing the isolation rate of bordetella pertussis during a whooping cough epidemic in west glamorgan, wales, are reported. the organism was isolated from 39% of patients with clinical whooping cough, pernasal swabbing being much more successful than cough plates. isolation rates were increased in the non-immunized, particularly in the first year of life. erythromycin and co-trimoxazole significantly reduced the isolation rate of b. pertussis but this did not occur with p ... | 1983 | 6300227 |
| stimulation and inhibition of adenylyl cyclases mediated by distinct regulatory proteins. | adenylyl cyclases are under positive and negative control by guanine nucleotides and hormones. stimulatory responses are mediated by a guanine nucleotide- and mg-binding regulatory component (ns), a protein that has been purified to homogeneity. inhibitory responses have been hypothesized to be mediated by an analogous regulatory component (ni) distinct from ns, but definitive proof for this is lacking and these effects may result from modulation of ns activity. recently, bordetella pertussis to ... | 1983 | 6300694 |
| delayed hypersensitivity induced by anti-t-cell-line antisera is enhanced by pertussigen and not restricted by histocompatibility genes. | anti-t-cell-line antisera were raised by repeatedly injecting mice with syngeneic, antigen-specific, delayed hypersensitivity (dh) inducing cells grown as continuous t-cell lines in vitro. many of these antisera could induce antigen-specific dh responses which, in some cases, were rather slight. for example, the dh reaction to azobenzenearsonate induced in a/j mice by an a/j antiserum produced against the syngeneic azobenzenearsonate-specific cell line aa3 was only a weak response. administratio ... | 1983 | 6300889 |
| [role of the protein fraction of bordetella pertussis in inducing experimental allergic encephalomyelitis]. | b. pertussis protein fraction obtained by precipitation with trichloroacetic acid (tca) stimulated the development of clinically and histologically pronounced experimental allergic encephalomyelitis (eae) in guinea pigs when introduced together with a heterologous cerebral antigen, the two compounds forming an encephalitogenic mixture. the adjuvant activity of the tca-precipitated fraction depended on its dose. the sera of the animals with eae induced by the encephalitogenic mixture containing b ... | 1983 | 6301173 |
| bacterial cell wall components decrease the number of guinea-pig lung beta-adrenoceptors. | infections of the deeper respiratory airways can contribute to the progression of chronic asthmatic bronchitis. in the present report a number of microorganisms affecting the number of beta-adrenoceptors in guinea-pig lung homogenates are described. haemophilus influenzae, streptococcus pneumoniae, bordetella pertussis and escherichia coli o111b4 induced a significant decrease of the number of beta-adrenoceptors (by approximately 20%). staphylococcus aureus, influenza a virus and escherichia col ... | 1983 | 6301848 |
| synergistic effect of bordetella pertussis lymphocytosis-promoting factor on protective activities of isolated bordetella antigens in mice. | the effect of low levels of added lymphocytosis-promoting factor (lpf) on the ability of several antigenic preparations isolated from bordetella pertussis and other bacteria to protect mice against intracerebral infection with b. pertussis was examined. lpf was found to enhance the protective activities of filamentous hemagglutinin, 22s antigen, and fimbriae isolated from b. pertussis. outer membrane protein preparations from phase i b. pertussis which had lpf removed by haptoglobin affinity col ... | 1983 | 6301999 |
| [toxicity of bordetella pertussis suspensions associated with the preparation of pertussis vaccine]. | the process of the detoxication of b. pertussis suspensions during their storage has a wave-like character and is determined by changes in the levels of the toxicity of the soluble and corpuscular fractions. conditions facilitating the transition of toxic cellular products into the soluble state may lead to the increase of the toxic activity of pertussis vaccines. | 1983 | 6303021 |
| identification of 2-amino-6-o-(2-amino-2-deoxy-beta-d-glucopyranosyl)-2-deoxy-d-glucose as a major constituent of the hydrophobic region of the bordetella pertussis endotoxin. | | 1983 | 6303589 |
| calcium-independent activation of adenylate cyclase by calmodulin. | adenylate cyclase of bordetella pertussis is stimulated by calmodulin by two distinct interactions. at low activator concentrations (approximately equal to 1 nm) the process is ca2+-dependent (i.e. inhibited by egta added before calmodulin). high activator concentrations (approximately equal to 0.1-10 microm) stimulate adenylate cyclase also in the presence of egta, an effect not accounted for by residual ca2+ or low concentrations of ca x calmodulin, which thus appears to be due to calcium-free ... | 1983 | 6303782 |
| [detection of antibody against bordetella pertussis in whooping cough patients, healthy children and newborn infants. igg, igm and iga antibody responses determined by enzyme-linked immunosorbent assay]. | | 1983 | 6304209 |
| enhancement of the intensity, persistence, and passive transfer of delayed-type hypersensitivity lesions by pertussigen in mice. | pertussigen, a purified protein from bordetella pertussis, was shown to increase delayed-type hypersensitivity (dth) to protein antigens in mice. first, it caused an approximately twofold enhancement of the magnitude of 24-h dth reactions. second, the peak magnitude of dth was delayed to 4-7 d after challenge, at which time it was five times more intense than in mice not receiving pertussigen. this reaction was antigen specific, and histologically was characterized by a dense mononuclear infiltr ... | 1983 | 6304228 |
| bordetella pertussis toxins. | | 1982 | 6304790 |
| separation and purification of the hemagglutinins from bordetella pertussis. | the role of the filamentous hemagglutinin (fha) and the lymphocytosis-promoting factor hemagglutinin (lpf) in pertussis pathogenesis and immunity is the subject of active investigation. to be certain of their role as protective antigens, the hemagglutinins must be pure and free of each other. this report describes procedures to separate and purify fha and lpf from the culture supernatant of stationary cultures of bordetella pertussis tohama, using hydroxylapatite, haptoglobin-sepharose, and seph ... | 1983 | 6305841 |
| novel biological property of pertussis toxin: chemotactic activity on human monocytes. | pertussis toxin isolated from bordetella pertussis phase i strain tohama enhanced the migration of human blood monocytes in an assay with a multiwell chemotaxis assembly. the monocyte migration enhancement by pertussis toxin was abolished by heating at 100 degrees c for 30 min, which caused a complete loss of the other biological activities of pertussis toxin. checkerboard analysis demonstrated that the increased monocyte migration caused by pertussis toxin was directed toward a positive stimulu ... | 1983 | 6305846 |
| heptakis(2,6-o-dimethyl)beta-cyclodextrin: a novel growth stimulant for bordetella pertussis phase i. | the effect of cyclodextrins on the growth of bordetella pertussis tohama phase i in synthetic medium was evaluated. the addition of cyclodextrins, especially heptakis(2,6-o-dimethyl)beta-cyclodextrin (me beta cd), to a complete synthetic medium such as stainer-scholte medium gave the same number of individual colonies and growth rates as those on bordet-gengou medium. furthermore, with the addition of me beta cd, growth inhibition by fatty acids such as oleic or palmitic acid was overcome and no ... | 1983 | 6306047 |
| in vitro induction of altered response of rat atria to acetylcholine with histamine-sensitizing factor of bordetella pertussis. | purified histamine-sensitizing factor (hsf) of bordetella pertussis induced in vitro an alteration in the pharmacologic response of rat atria to acetylcholine. spontaneously beating atrial preparations isolated from rats were exposed to hsf at a concentration of 50 ng/ml at 37 c for 30 min to 4 hr and washed with krebs-ringer solution, and then tested at 1-hr intervals up to 28 hr during incubation for their responses to epinephrine and acetylcholine. at 13 hr after exposure to hsf, irrespective ... | 1983 | 6306409 |
| [proliferation of spleen cells in response to bordetella pertussis]. | vaccine strain 305 of b. pertussis in a dose of 10(8)-10(11) cells was shown to be mitogenic for splenocytes of balb/c mice and nude mice. when added in a dose of 10(10) b. pertussis exerted a more pronounced mitogenic effect than phytohemagglutinin p, which was less powerful, however, than that of con a, b. pertussis caused a greater stimulation of dna synthesis in lymphocytes than b mitogens whose action depended on the differentiation stages of b lymphocytes. this is likely to hint towards a ... | 1983 | 6307420 |
| impaired autonomic responsiveness of the cardiovascular system of the rat induced by a heat-labile component of bordetella pertussis vaccine. | bordetella pertussis vaccination induces severe impairment of the autonomic responsiveness of the cardiovascular system in rats. the vasodilation after beta 2-adrenoceptor stimulation with salbutamol as well as the negative chronotropic action induced by the muscarinic receptor stimulant arecoline were inhibited 4 days after vaccination. moreover, basal blood pressure values appeared to be significantly lower in b. pertussis-vaccinated rats compared with control animals. these effects were depen ... | 1983 | 6307869 |
| modulation of bordetella pertussis by nicotinic acid. | growth of bordetella pertussis in a high concentration of nicotinic acid (na) had a modulating effect on several properties and activities of the bacteria. compared with normally grown cells, those grown in a high concentration of na had reduced capacity for taking up both na and nicotinamide (nd); they had reduced adenylate cyclase activity and showed loss of agglutinogen factors 2 and 3, but an increase in factor 1. by contrast, cells grown in a high concentration of nd showed only a slightly ... | 1983 | 6307872 |
| in-vitro sensitivity of bordetella pertussis. | the in-vitro effect of ampicillin, chloramphenicol, clindamycin, erythromycin, trimethoprim and trimethoprim-sulphamethoxazole against 152 strains of bordetella pertussis was investigated. when tested against erythromycin, 96% of the isolated strains were inhibited by less than or equal to 1 mg/l. the other antibiotics did not inhibit the growth of bord. pertussis at concentrations judged achievable in-vivo. | 1983 | 6307963 |
| pertussis toxin substrate, the putative ni component of adenylyl cyclases, is an alpha beta heterodimer regulated by guanine nucleotide and magnesium. | the final step in a scheme for the purification of the guanine nucleotide- and mg2+-binding stimulatory regulatory component (ns) of adenylyl cyclase [adenylate cyclase; atp pyrophosphate-lyase (cyclizing), ec 4.6.1.1] from human erythrocyte membranes involves chromatography over hydroxylapatite (hap) which yields two fractions. the first fraction (hap i) contains predominantly two peptides that, upon sodium dodecyl sulfate/polyacrylamide gel electrophoresis, migrate with mr values of 39,000 and ... | 1983 | 6308612 |
| [importance of immunoelectrophoresis in agar and disc electrophoresis in polyacrylamide gel for characterizing different strains of bordetella pertussis]. | the antigenic composition of typical and atypical b. pertussis strains obtained in the foci of pertussis infection, as well as experimentally obtained antibiotic-resistant b. pertussis strains, has been studied by the methods of immunoelectrophoresis in agar and electrophoresis in polyacrylamide gel (paag). immunoelectrophoresis in agar has been found capable of differentiating b. pertussis culture from a group of unidentified morphologically similar gram-negative bacilli by their antigenic comp ... | 1983 | 6308926 |
| effect of heptakis (2,6-o-dimethyl) beta-cyclodextrin on the production of pertussis toxin by bordetella pertussis. | the effect of heptakis (2,6-o-dimethyl) beta-cyclodextrin (me beta cd) on the production of pertussis toxin was evaluated. the addition of me beta cd to the medium stimulated cell growth and pertussis toxin production. me beta cd enhanced pertussis toxin production 100 times more in synthetic media, such as stainer-scholte medium (d. w. stainer and m. j. scholte, j. gen. microbiol. 63:211-220), than in me beta cd-free medium in 2-day shake cultures. maximum production of pertussis toxin was esti ... | 1983 | 6309661 |
| serological response to filamentous hemagglutinin and lymphocytosis-promoting toxin of bordetella pertussis. | serum antibody responses to the filamentous hemagglutinin and the lymphocytosis-promoting toxin of bordetella pertussis after vaccination with diphtheria and tetanus toxoids and pertussis vaccine, adsorbed, were assayed by using the enzyme-linked immunosorbent assay. the effect of early immunization, during the first week of life, on the antibody response also was determined. after vaccination, immunoglobulin g (igg) and igm directed against both the filamentous hemagglutinin and the lymphocytos ... | 1983 | 6309662 |
| the subunits of the stimulatory regulatory component of adenylate cyclase. resolution, activity, and properties of the 35,000-dalton (beta) subunit. | the stimulatory guanine nucleotide-binding regulatory component (g/f) of adenylate cyclase is activated by exposure to guanine nucleotide analogs or to al3+ + f-. activated g/f can reconstitute adenylate cyclase activity when mixed with the catalytic moiety of the enzyme system in the absence of an effective free concentration of stimulatory ligand. activation is explained by dissociation of the alpha (45,000-da) and beta (35,000-da) subunits of g/f. the beta subunit of g/f facilitates reversal ... | 1983 | 6309843 |
| adherence of bordetella pertussis to human respiratory epithelial cells. | adherence of bordetella pertussis to ciliated respiratory tract mucosa is important in the pathogenesis of whooping cough. the adherence of b pertussis to human respiratory epithelial cells was investigated using cells obtained by brushing at bronchoscopy. b pertussis attached exclusively to the ciliary tufts of ciliated cells. a mean +/- sem of 5.0 +/- 0.3 organisms attached per cell when bacteria in a concentration of 2 x 10(9)/ml were incubated with ciliated cells. organisms examined by elect ... | 1983 | 6309991 |
| transplacental and transcolostral immunity to pertussis in a mouse model using acellular pertussis vaccine. | the protective activity of acellular pertussis vaccine was comparable to that of whole cell vaccine against intracerebral and aerosol infection with bordetella pertussis in mice. suckling mice, six to 10 days old, born of mothers immunized with acellular or whole cell pertussis vaccine were challenged with an aerosol of b pertussis strain 18323, and body weight, death, leukocytosis, and viable counts in the lungs were monitored. protective activity was transferred from mother mice to their offsp ... | 1983 | 6309992 |
| tn5-induced mutations affecting virulence factors of bordetella pertussis. | transposon tn5 was used to isolate mutants of bordetella pertussis. strains with tn5 insertions were screened for loss of virulence-associated factors, including filamentous hemagglutinin, hemolysin, and pertussis toxin. several mutants deficient for hemolysin production were obtained. all produced dermonecrotic toxin, pertussis toxin, and filamentous hemagglutinin, but were found to vary with respect to adenylate cyclase production. one hemolysin mutant had no detectable adenylate cyclase activ ... | 1983 | 6311749 |
| activation by thiol of the latent nad glycohydrolase and adp-ribosyltransferase activities of bordetella pertussis toxin (islet-activating protein). | pertussis toxin (islet-activating protein) activates adenylate cyclase in susceptible cells by adp-ribosylating an inhibitory component of the cyclase system. this toxin, assayed in a cell-free system in the presence of high concentrations of thiol, catalyzed the hydrolysis of nad to adp-ribose and nicotinamide. this nad glycohydrolase activity co-chromatographed on sephacryl g-200 in 6.5 m urea, ph 3.2, 0.1 m glycine with the adp-ribosyltransferase activity of the toxin, as monitored by the tra ... | 1983 | 6311827 |
| bordetella pertussis isolates studied at the national health institute, wellington, between 1977 and 1981. | between 1977 and 1982, 199 isolates of bordetella pertussis were referred to the national health institute for confirmation of identity and from mid 1982 for serotyping. the information gained from cultures referred during 1982 has been of assistance to the new zealand department of health in investigating the causes of the present outbreak and has also enabled comparison with the serotypes involved in the present outbreak in the united kingdom. | 1983 | 6312385 |
| heterogeneity of the filamentous haemagglutinin of bordetella pertussis studied with monoclonal antibodies. | the filamentous haemagglutinin of bordetella pertussis has been purified from static, liquid culture supernatants and from extracts of cells grown on a solid medium. sds-page of the purified protein has shown multiple polypeptides with molecular weights ranging from 220 000 to about 58 000. by transferring the sds-dissociated polypeptides to nitrocellulose paper and reacting with several monoclonal antibodies, it has been shown that many of the polypeptides are probably fragments of the polypept ... | 1983 | 6313862 |
| [cloning of bordetella pertussis dna in an escherichia coli bacterial cell system]. | hybrid plasmids containing b. pertussis dna insertions have been constructed with the use of the vector plasmid pbr 322 and the pst i fragments of b. pertussis dna. some properties of the hybrid plasmids are characterized. the possibility of the expression of b. pertussis genes in the protein-synthetizing cell-free system obtained from e. coli has been demonstrated. | 1983 | 6314709 |
| pokeweed mitogen, bordetella pertussis and breast milk cell factor induce preferentially the synthesis of different immunoglobulin classes. | human tonsillar lymphocytes were cultured in the presence of different activators and [14c]-isoleucine. de novo synthesized, [14c]-labeled immunoglobulin was determined after separation of the different classes by immunoadsorbants carrying class-specific anti-human iga, igg, igm, igd or ige. pokeweed mitogen and whole killed bordetella pertussis enhanced the synthesis and secretion of iga, igg and igm. maximum stimulation was found with pokeweed mitogen in igm secretion (up to 5-fold), while bor ... | 1983 | 6315033 |
| ornithine-containing lipid of bordetella pertussis, a new type of hemagglutinin. | the ornithine-containing lipids of six strains (phases i-iv) of bordetella pertussis were prepared from the total extractable cellular lipids by thin-layer chromatography and treatment with phospholipase a. they were compared with those prepared from two strains each of bordetella parapertussis and bordetella bronchiseptica. the structures of the ornithine-containing lipid of b. pertussis and the other two species were resolved by acid and alkaline hydrolysis, gas-liquid chromatography, infrared ... | 1983 | 6315433 |
| species specificity of bordetella adherence to human and animal ciliated respiratory epithelial cells. | bacteria of the genus bordetella adhere preferentially to ciliated respiratory epithelial cells. we investigated the specificity of this unique tropism by assessing the concentration-dependent adherence of the three bordetella species to ciliated cells from different hosts. bordetella pertussis and bordetella parapertussis adhere better to human ciliated cells than to those from rabbits, mice, or hamsters. in contrast, bordetella bronchiseptica demonstrates preferential adherence to nonhuman mam ... | 1983 | 6315583 |
| pertussis toxin. affinity purification of a new adp-ribosyltransferase. | pertussis toxin, the major toxin produced by bordetella pertussis, catalyzes the adp-ribosylation of a specific membrane polypeptide which appears to be involved in regulation of the catalytic subunit of adenylate cyclase. in the current study, a rapid purification procedure has been developed for the preparation of pertussis toxin in high yields. through the sequential use of the affinity matrices affi-gel blue and fetuin-sepharose 4b, milligram quantities of apparently homogeneous toxin can be ... | 1983 | 6315733 |
| serum iga antibody to bordetella pertussis as an indicator of infection. | the levels of pertussis-specific iga antibodies in sera from vaccinees and from children with bordetella pertussis infection were compared by an enzyme-linked immunosorbent assay (elisa). serum iga antibodies were produced only after natural contact with the pathogen and, therefore, their presence can be used as an indicator of infection. however, in view of the relatively long interval between infection and the appearance of antibodies, and the prolonged antibody response, their presence cannot ... | 1983 | 6315942 |
| vaccination of genetically susceptible mice against chronic infection with nematospiroides dubius using pertussigen as adjuvant. | the intestinal nematode nematospiroides dubius persists for prolonged periods in mice after a single oral administration of infective larvae. after multiple administrations of larvae, some inbred mouse strains (termed 'resistant') develop the capacity to expel the majority of adult worms from the intestines. other mouse strains such as cba/h and c57bl/6 (and, in particular, males) are far more susceptible in that they expel few, if any, adult worms after repeated doses of larvae and may die with ... | 1983 | 6316887 |
| isolation and characterization of bordetella pertussis phenotype variants capable of growing on nutrient agar: comparison with phases iii and iv. | unsupplemented nutrient agar (na) was used to select spontaneous phenotype variants (pvs) of bordetella pertussis tohama i and 3779 which, by their growth on na, could possibly be considered equivalent to phase iv in the system of leslie and gardner (p.h. leslie and a.d. gardner, j. hyg. 31:423-434, 1931) or phase iii in the system of kasuga et al. (t. kasuga, y. nakase, k. ukishima, and k. takatsu, kitasato arch. exp. med. 26:121-134, 1953). na growers (gna+) were selected from the flat, nonhem ... | 1984 | 6317566 |
| two physically and serologically distinct lipopolysaccharide profiles in strains of bordetella pertussis and their phenotype variants. | the lipopolysaccharide (lps) from nine strains representing 18 phenotype variants of bordetella pertussis could be grouped into one of two distinct profiles by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining. one group, representing the wild-type lps profile of b. pertussis, consisted of two silver-staining bands: a dominant brown-amber a band and a faster-migrating, minor, black-staining b band. the second group, representing a variant lps profile, consisted of a s ... | 1984 | 6317567 |
| genetic analysis of phase change in bordetella pertussis. | avirulent-phase derivatives of bordetella pertussis (those which have simultaneously lost the ability to synthesize several virulence-associated factors) and the genetic mechanism of the phase change were studied. increased tolerance to erythromycin was shown to be an avirulent-phase marker. by the use of efficiency of plating on erythromycin, the proportion of avirulent-phase (vir) variants in a virulent-phase (vir+) population was determined to be between 10(-3) and 10(-6), depending on the st ... | 1984 | 6317569 |
| [the camp system and bacterial toxins]. | the effects of cytotoxic proteinaceous bacterial toxins on the adenylate cyclase cyclic adenosine monophosphate (camp) system of host cells are reviewed. bacterial exotoxins affecting intracellular camp levels of host cells can be classified into two subgroups: (1) adenosine diphosphate (adp)-ribosyl-transferases and, (2) invasive adenylate cyclases. among the adp-ribosylating toxins are the enterotoxins of vibrio cholerae, of escherichia coli and one of the pertussis toxins termed "islet-activa ... | 1983 | 6317939 |
| immunologic response to early and routine dtp immunization in infants. | the effect of early immunization, prior to discharge from the newborn nursery, on subsequent immunity as determined by enzyme-linked immunosorbent assay (elisa) immunoglobulin (ig) m and igg antibody titers to filamentous hemagglutinin and lymphocytosis-promoting toxin (lpt) of bordetella pertussis and by standard pertussis agglutinin titers was investigated. eighteen infants received routine diphtheria-tetanus-pertussis (dtp) immunization at 2, 4, and 6 months of age; 17 other infants received ... | 1984 | 6318185 |
| [improved technology of culturing bordetella pertussis based on a study of the kinetics of the process]. | the article deals with the study of the kinetics of the processes occurring in the population of b. pertussis grown in a semisynthetic culture medium. on the basis of the mathematical description of the specific growth rate the factors permitting the regulation of the processes of the cultivation of b. pertussis have been revealed. new prolonged multicyclic combined exponential and continuous processes of the cultivation of b. pertussis have been worked out and introduced into practice. these pr ... | 1983 | 6318479 |
| [evaluation of the indices of humoral and cellular immunity induced in mice of various genotypes by pertussis antigens]. | the genetic predetermination of the level of immune reactions to the injection of corpuscular pertussis vaccine and purified soluble pertussis vaccine has been revealed. genetic differences in the intensity of immune response are manifest in both humoral and cell-mediated immunity. c57bl/6 mice belong to strains with high responsiveness, while nihhsfs/h and cbwa mice belong to strains with low responsiveness to bordetella pertussis. | 1983 | 6318483 |
| adp-ribosylation of adenylate cyclase by pertussis toxin. effects on inhibitory agonist binding. | adenylate cyclase in ng108-15 (neuroblastoma x glioma hybrid) cells is responsive to both stimulatory and inhibitory ligands. bordetella pertussis toxin (pt) catalyzes the adp-ribosylation of a 41,000-da peptide believed to be a subunit of the putative guanyl nucleotide-binding protein (gi) involved in cyclase inhibition and abolishes inhibitory effects of opiate agonists. in studying the effects of pt on opiate receptors, we found that [3h]enkephalinamide binding was reduced by approximately 90 ... | 1984 | 6319376 |
| humoral immunity in essential fatty acid-deficient rats and mice: effect of route of injection of antigen. | weanling male lewis mai f rats and a/j mice were fed semi-purified diets either adequate or deficient in essential fatty acids (efa) for 50-60 days. after death livers were excised, the lipids were extracted, and the fatty acid profile was determined. groups of rats and mice were immunized by injection with sheep red blood cells (srbc) either i.v. or i.p. one group of rats received an injection of srbc plus bordetella pertussis organisms. the plaque-forming cell response (pfc) of all groups was ... | 1984 | 6319645 |
| production and properties of bordetella pertussis heat-labile toxin. | twelve selected strains of bordetella pertussis were compared quantitatively for their ability to produce heat-labile toxin (hlt); all proved to be active producers, with only a three-fold range between the highest and the lowest. bordet-gengou agar, charcoal agar, modified hornibrook medium and stainer and schölte (12g) medium differed little in their ability to support toxin production by three b. pertussis strains. however, cells grown on the solid media for 24 h were slightly more toxic than ... | 1984 | 6319705 |
| pertussis toxin decreases opiate receptor binding and adenylate inhibition in a neuroblastoma x glioma hybrid cell line. | neuroblastoma x glioma hybrid cells ng108-15 were treated with a toxin derived from bordetella pertussis. as compared to control cells grown in the absence of toxin, the inhibitory effects of opioid agonists upon camp formation were dose-dependently impaired by a non-competitive mechanism. radioligand binding studies revealed that opioid agonist binding was dramatically reduced in toxin-treated membranes when tested in the presence of na+/mg++/gmp-pnp. further, the potencies of guanine nucleotid ... | 1983 | 6319865 |
| toxicity testing of pertussis vaccines: effect of increased sensitivity of mice to bordetella pertussis. | the increased sensitivity to bordetella pertussis of a line of cd-1 mice used in toxicity-testing and the consequent and needless rejection of several lots of dpt vaccine are described. apart from changes in the mice the possible presence of an infective agent in the animal house could not be discounted. the usefulness of individual mouse weights in the assay as opposed to group weights and of a reference preparation to monitor the performance of mice is discussed. | 1984 | 6321509 |
| effects of bordetella pertussis components on ige and igg1 responses. | the effect of dermonecrotic toxin (dnt), fimbrial hemagglutinin (fha), k-agglutinogen, lipopolysaccharide (lps), and pertussigen from bordetella pertussis on the production of ige and igg1 antibodies to hen egg albumin (ea) was investigated in c57bl/6 mice. the ige antibody contents were determined by passive cutaneous anaphylaxis (pca) in the skin of lewis rats, while the igg1 antibody contents were determined by pca reactions on the skin of mice using sera that had been heated for 3 hr at 56 c ... | 1983 | 6321910 |
| pleurorrhea-inducing activity of the histamine-sensitizing factor of bordetella pertussis. | | 1983 | 6321914 |
| the inhibitory guanine nucleotide-binding regulatory component of adenylate cyclase. properties and function of the purified protein. | treatment of membranes with islet activating protein (iap), a toxin from bordetella pertussis, results in abolition of gtp-dependent, receptor-mediated inhibition of adenylate cyclase. this appears to result from iap-catalyzed adp-ribosylation of a 41,000-da membrane-bound protein. a protein with 41,000- and 35,000-da subunits has been purified from rabbit liver membranes as the predominant substrate for iap. this protein has now been shown to be capable of regulating membrane-bound adenylate cy ... | 1984 | 6323430 |
| preliminary evidence for a pyridine nucleotide cycle in bordetella pertussis. | preliminary evidence that bordetella pertussis has a functional pyridine nucleotide cycle was the observation that [14c]-nicotinic acid was rapidly metabolized during its uptake by the bacteria to pyridine nucleotides and nicotinamide. nicotinamide deamidase activity, necessary for the completion of the cycle by conversion of nicotinamide to nicotinic acid, was found in a soluble extract (20 000 x g supernatant) of b. pertussis cell lysates. | 1984 | 6326669 |
| serum antibody responses to the outer membrane proteins of bordetella pertussis. | the serum antibody responses to the outer membrane proteins, purified filamentous hemagglutinin, and leukocytosis-promoting factor of bordetella pertussis were examined in mice and children immunized with pertussis whole-cell vaccine. it was found that, although there were many similarities in the responses of mice and children, there were important differences. sera from vaccinated mice reacted strongly with purified filamentous hemagglutinin and gave weak or undetectable responses to the compo ... | 1984 | 6327532 |
| diagnosis of pertussis. | during a local outbreak of pertussis mainly involving school-children and adults, 71 patients with suspected whooping cough and 25 of their household contacts were studied in the course of 1 day so as to compare enzyme-linked immunosorbent assay (elisa) with bacterial isolation in the diagnosis of pertussis. serum samples and two nasopharyngeal swabs were collected from all persons. five different culture media were used. twenty-eight cases were diagnosed within 2 days by measuring igm- and iga- ... | 1984 | 6327829 |
| distribution of radioactivity in rats injected intravenously with 125i-labeled histamine-sensitizing factor of bordetella pertussis. | | 1984 | 6328231 |
| bordetella pertussis: multiple attacks on host cell cyclic amp regulation. | | 1984 | 6328913 |
| bordetella adenylate cyclase toxin: entry of bacterial adenylate cyclase into mammalian cells. | we have identified an adenylate cyclase toxin in urea extracts and culture supernatant fluids of bordetella pertussis (2). the ability of this toxin and the lack of a strong correlation between its activity and adenylate cyclase activity found in urea extracts suggest that it is an oligomer of readily dissociable subunits. the mechanism by which bordetella adenylate cyclase toxin interacts with target cells is unknown, but polyvalent cations are necessary. neutrophils exposed to the toxin acquir ... | 1984 | 6328914 |
| antibiotic resistance of degraded strains of bordetella pertussis. | the susceptibilities to erythromycin, rifampin, polymyxin b, ampicillin, tetracycline, gentamicin, fusidic acid, trimethoprim, and spectinomycin of five virulent phase i strains of bordetella pertussis and their degraded phase iv descendants were compared. increases in mics of 2- to 16-fold were observed for erythromycin, rifampin, tetracycline, fusidic acid, trimethoprim, and spectinomycin for four of the five degraded strains. | 1984 | 6329086 |
| adoptive transfer of experimental allergic encephalomyelitis in mice with the aid of pertussigen from bordetella pertussis. | adoptive transfer of experimental allergic encephalomyelitis (eae) in (sjl x balb/c)f1 mice was accomplished by an iv injection of 2.4 to 4.7 x 10(7) lymph node cells (lnc) from mice immunized with mouse spinal cord emulsified in complete freund's adjuvant when both donors and recipients had been treated iv with 400 ng of pertussigen at the time of immunization for the donors and on transfer of cells for the recipients. pertussigen was essential in both donors and recipients for development of f ... | 1984 | 6329525 |
| interleukin 1 secretion by human monocytes stimulated by the isolated polysaccharide region of the bordetella pertussis endotoxin. | the isolated polysaccharide chain, ps-1, of the bordetella pertussis endotoxin was examined by isoelectric focusing, sds-polyacrylamide gel electrophoresis and gel filtration for heterogeneity and for possible contamination by the parent endotoxin. this polysaccharide, previously found to be a very potent, macrophage-dependent, polyclonal b-cell activator and to mediate the specific binding of the endotoxin to macrophages, stimulated the interleukin 1 (il 1) secretion by human monocytes; its pot ... | 1984 | 6330537 |
| a new method of testing anti-allergic drugs. | rats were sensitized with a single intraperitoneal dose of bovine serum albumin in alhydrogel plus bordetella pertussis vaccine, and local anaphylaxis was elicited in the paw by soluble antigen 2 weeks later. the response was mainly due to ige-type antibodies and proved to be highly sensitive to beta-adrenoceptor agonists. dexamethasone inhibited the response after a lag phase. methysergide, disodium chromoglycate, diethylcarbamazine, bw 775/c, nordihydroguarjaretic acid, and fpl 55712 were also ... | 1983 | 6341256 |
| host immune status in uremia. iv. phagocytosis and inflammatory response in vivo. | infection is a frequent complication and cause of death in renal failure, but the association between uremia, depressed immune status, and susceptibility to infection is far from proven. in the present studies, the effect of uremia on the inflammatory response and phagocytic ability was investigated in an animal model. the inflammatory response, as measured by the ability of leukocytes to mobilize into subcutaneous implanted sponges, was impaired at 6 hr but was normal 24 hr after implantation. ... | 1983 | 6341683 |
| [prospects for the vaccinal prevention of haemophilus influenzae type b meningitis]. | haemophilus influenzae type b remains the major cause of bacterial meningitis in infants and children. serum antibodies against the capsular polysaccharide--polyribosylribitolphosphate (prp)--are protective, but its immunogenicity is poor in children under two years of age. clinical trials actually in progress concerning an association of prp with bordetella pertussis are presented. other vaccinal preparations are possible, such outer membrane protein, but prospects offered by polysaccharide-pro ... | 1983 | 6341945 |
| enhanced glucose-induced insulin release and endogenous ca2+ ionophoretic activity in neonatal rat islets following islet-activating protein. | islet-activating protein, purified from the culture medium of bordetella pertussis, enhanced glucose-induced insulin secretion from cultured neonatal rat islets in a calcium dependent manner. this effect was accompanied by an increase in lipid associated ca2+ ionophoretic activity, as measured by passage of ca2+ through multilamellar planar membranes containing islet lipid extracts. these findings suggest that the action of iap in the neonatal islet may be mediated by enhanced entry of extracell ... | 1983 | 6342618 |