| case-control study shows that neonatal pneumococcal meningitis cannot be distinguished from group b streptococcus cases. | streptococcus pneumoniae (s. pneumoniae) is sometimes implicated in neonatal bacterial meningitis. this study described the demographic, clinical and biological features of neonatal s. pneumoniae meningitis and compared pneumococcal and group b streptococcal (gbs) neonatal meningitis. | 2017 | 28508419 |
| preparation and properties of carbohydrate-based composite films incorporated with cuo nanoparticles. | the present study aimed to develop the carbohydrate biopolymer based antimicrobial films for food packaging application. the nanocomposite films of various biopolymers and copper oxide nanoparticles (cuonps) were prepared by solvent casting method. the nanocomposite films were characterized using sem, ftir, xrd, and uv-vis spectroscopy. the thermal stability, uv barrier, water vapor permeability, and antibacterial activity of the composite films were also evaluated. the surface morphology of the ... | 2017 | 28504145 |
| effects of sorbic acid-chitosan microcapsules as antimicrobial agent on the properties of ethylene vinyl alcohol copolymer film for food packaging. | this paper discusses the possibility of using sorbic acid-chitosan microcapsules (s-mps) as an antibacterial component of active ethylene vinyl alcohol copolymer (evoh) film. s-mps with a diameter of approximately 1 to 4 μm showed a sorbic acid loading capacity of 46.5%. addition of s-mps (3%, w/w) increased the tensile strength, haze, oxygen, and water vapor barrier properties, as well as reduced the elongations at break and transmittance of s-mps-evoh (s-mp-evoh) film. antibacterial tests show ... | 2017 | 28488775 |
| etiology and laboratory abnormalities in bacterial meningitis in neonates and young infants. | we conducted a retrospective review of electronic medical records of all cases of bacterial meningitis in neonates and young infants at our institution from 2004 to 2014. fifty-six cases were identified. the most common causative organism was group b streptococcus, followed by escherichia coli and then listeria monocytogenes. forty-four of the 56 patients in the study had abnormalities of the blood white blood cell (wbc) count. the most common wbc count abnormalities were leukopenia and elevatio ... | 2017 | 28484584 |
| formulation and shelf-life of fish burgers served to preschool children. | consumer is very careful about healthiness; in this context nutritionists often highlight the importance of fish for human nutrition because of their protein and fatty acid composition. in order to stimulate utilisation and consumption of fish species by unusual target groups such as children, the aim of this research was to formulate and to evaluate shelf-life and nutritional values of fish preparations stored in modified atmosphere packaging (map). fish species used for trail were trachurus tr ... | 2017 | 28462206 |
| short communication: microbiological quality of raw cow milk and its association with herd management practices in northern china. | contamination of raw milk with bacterial pathogens is potentially hazardous to human health. the aim of this study was to evaluate the total bacteria count (tbc) and presence of pathogens in raw milk in northern china along with the associated herd management practices. a total of 160 raw milk samples were collected from 80 dairy herds in northern china. all raw milk samples were analyzed for tbc and pathogens by culturing. the results showed that the number of raw milk samples with tbc <2 × 10( ... | 2017 | 28434737 |
| application of electrolysed oxidising water as a sanitiser to extend the shelf-life of seafood products: a review. | electrolysed oxidising water (e.o. water) is produced by electrolysis of sodium chloride to yield primarily chlorine based oxidising products. at neutral ph this results in hypochlorous acid in the un-protonated form which has the greatest oxidising potential and ability to penetrate microbial cell walls to disrupt the cell membranes. e.o. water has been shown to be an effective method to reduce microbial contamination on food processing surfaces. the efficacy of e.o. water against pathogenic ba ... | 2017 | 28416883 |
| bacterial attachment and biofilm formation on surfaces are reduced by small-diameter nanoscale pores: how small is small enough? | prevention of biofilm formation by bacteria is of critical importance to areas that directly affect human health and life including medicine, dentistry, food processing and water treatment. this work showcases an effective and affordable solution for reducing attachment and biofilm formation by several pathogenic bacteria commonly associated with foodborne illnesses and medical infections. | 2015 | 28721236 |
| sulfadiazine-chitosan conjugates and their polyelectrolyte complexes with hyaluronate destined to the management of burn wounds. | in the present study polyelectrolyte complexes (pecs) based on new sulfadiazine-chitosan conjugates with sodium hyaluronate have been developed with potential use in treatment of burn wounds. the pecs were chemically characterized using fourier transform-infrared spectroscopy, scanning electon microscopy and near infrared chemical imaging technique. the swelling behavior and in vitro sulfadiazine release were also investigated. the antimicrobial activity was evaluated towards three bacterial str ... | 2015 | 28787940 |
| comparison among the quantification of bacterial pathogens by qpcr, dpcr, and cultural methods. | the demand for rapid methods for the quantification of pathogens is increasing. among these methods, those based on nucleic acids amplification (quantitative pcrs) are the most widespread worldwide. together with the qpcr, a new approach named digital pcr (dpcr), has rapidly gained importance. the aim of our study was to compare the results obtained using two different dpcr systems and one qpcr in the quantification of three different bacterial pathogens: listeria monocytogenes, francisella tula ... | 2017 | 28702010 |
| bacterial meningitis in finland, 1995-2014: a population-based observational study. | bacterial meningitis remains an important cause of morbidity and mortality worldwide. its epidemiological characteristics, however, are changing due to new vaccines and secular trends. conjugate vaccines against haemophilus influenzae type b and streptococcus pneumoniae (10-valent) were introduced in 1986 and 2010 in finland. we assessed the disease burden and long-term trends of five common causes of bacterial meningitis in a population-based observational study. | 2017 | 28592578 |
| estimated annual numbers of foodborne pathogen-associated illnesses, hospitalizations, and deaths, france, 2008-2013. | estimates of the annual numbers of foodborne illnesses and associated hospitalizations and deaths are needed to set priorities for surveillance, prevention, and control strategies. the objective of this study was to determine such estimates for 2008-2013 in france. we considered 15 major foodborne pathogens (10 bacteria, 3 viruses, and 2 parasites) and estimated that each year, the pathogens accounted for 1.28-2.23 million illnesses, 16,500-20,800 hospitalizations, and 250 deaths. campylobacter ... | 2017 | 28820137 |
| quantitative proteomic profiling for clarification of the crucial roles of lysosomes in microbial infections. | lysosomes play vital roles in both innate and adaptive immunity. it is widely accepted that lysosomes do not function exclusively as a digestive organelle. it is also involved in the process of immune cells against pathogens. however, the changes in the lysosomal proteome caused by infection with various microbes are still largely unknown, and our understanding of the proteome of the purified lysosome is another obstacle that needs to be resolved. here, we performed a proteomic study on lysosome ... | 2017 | 28433889 |
| the burden and epidemiology of community-acquired central nervous system infections: a multinational study. | risk assessment of central nervous system (cns) infection patients is of key importance in predicting likely pathogens. however, data are lacking on the epidemiology globally. we performed a multicenter study to understand the burden of community-acquired cns (ca-cns) infections between 2012 and 2014. a total of 2583 patients with ca-cns infections were included from 37 referral centers in 20 countries. of these, 477 (18.5%) patients survived with sequelae and 227 (8.8%) died, and 1879 (72.7%) p ... | 2017 | 28397100 |
| microbial vertical transmission during human pregnancy. | congenital infections with pathogens such as zika virus, toxoplasma gondii, listeria monocytogenes, treponema pallidium, parvovirus, hiv, varicella zoster virus, rubella, cytomegalovirus, and herpesviruses are a major cause of morbidity and mortality worldwide. despite the devastating impact of microbial infections on the developing fetus, relatively little is known about how pathogens associated with congenital disease breach the placental barrier to transit vertically during human pregnancy. i ... | 2017 | 28494237 |
| therapeutic options for treatment of human papillomavirus-associated cancers - novel immunologic vaccines: adxs11-001. | survival of patients with advanced, recurrent, or metastatic human papillomavirus (hpv)-associated cancer is suboptimal despite the availability of various treatment modalities. the recently developed bacterial vector listeria monocytogenes (lm) activates innate and adaptive immune responses and is expected to offer immunologic advantages. axalimogene filolisbac (axal or adxs11-001) is a novel immunotherapeutic based on the live, irreversibly attenuated lm fused to the nonhemolytic fragment of l ... | 2017 | 28725449 |
| a genetically modified attenuated listeria vaccine expressing hpv16 e7 kill tumor cells in direct and antigen-specific manner. | attenuated listeria monocytogenes (l. monocytogenes, lm) induces specific cd8(+) and cd4(+) t cell responses, and has been identified as a promising cancer vaccine vector. cervical cancer is the third most common cancer in women worldwide, with human papillomavirus (hpv), particularly type 16, being the main etiological factor. the therapeutic hpv vaccines are urgently needed. the e7 protein of hpv is necessary for maintaining malignancy in tumor cells. here, a genetically modified attenuated lm ... | 2017 | 28706878 |
| mechanistic insights into adxs11-001 human papillomavirus-associated cancer immunotherapy. | immune responses to the facultative intracellular bacterium listeria monocytogenes (lm) are robust and well characterized. utilized for decades as a model of host-disease immunology, lm is well suited for use as an immunotherapeutic bacterial vector for the delivery of foreign antigen. genetic modification of lm has been undertaken to create an attenuated organism that is deficient in its master transcriptional regulator, protein-related factor a, and incorporates a truncated, nonhemolytic versi ... | 2017 | 28588899 |
| aggregation-induced-emission materials with different electric charges as an artificial tongue: design, construction, and assembly with various pathogenic bacteria for effective bacterial imaging and discrimination. | imaging-based total bacterial count and type identification of bacteria play crucial roles in clinical diagnostics, public health, biological and medical science, and environmental protection. herein, we designed and synthesized a series of tetraphenylethenes (tpes) functionalized with one or two aldehyde, carboxylic acid, and quaternary ammonium groups, which were successfully used as fluorescent materials for rapid and efficient staining of eight kinds of representative bacterial species, incl ... | 2017 | 28809473 |
| himatanthus drasticus leaves: chemical characterization and evaluation of their antimicrobial, antibiofilm, antiproliferative activities. | plant-derived products have played a fundamental role in the development of new therapeutic agents. this study aimed to analyze antimicrobial, antibiofilm, cytotoxicity and antiproliferative potentials of the extract and fractions from leaves of himatanthusdrasticus, a plant from the apocynaceae family. after harvesting, h. drasticus leaves were macerated and a hydroalcoholic extract (hdhe) and fractions were prepared. antimicrobial tests, such as agar-diffusion, minimum inhibitory concentration ... | 2017 | 28561790 |
| synergistic effect of plantaricin bm-1 combined with physicochemical treatments on the control of listeria monocytogenes in cooked ham. | plantaricin bm-1, a typical iia bacteriocin, is produced by lactobacillus plantarum bm-1, which can be isolated from a traditionally fermented chinese meat product. this bacteriocin exhibits perfect thermal stability and broad inhibitory activity against certain foodborne pathogens. in this study, we investigated the effect of plantaricin bm-1 combined with physicochemical treatments (sodium nitrite, heat treatment, ultrahigh pressure technology) on the control of listeria monocytogenes populati ... | 2017 | 28467183 |
| lactococcus lactis and lactobacillus salivarius bgbu1-4 differently modulate early immunological response of wistar rats co-administered with listeria monocytogenes. | in the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. accordingly, the aim of this study was to test whether two lactic acid bacteria (lab) strains, lactococcus lactis bgbu1-4 and lactobacillus salivarius bgho1, could be applied as safe supplements for listeria infection. two major research objectiv ... | 2017 | 28856909 |
| structural and functional studies of pyruvate carboxylase regulation by cyclic di-amp in lactic acid bacteria. | cyclic di-3',5'-adenosine monophosphate (c-di-amp) is a broadly conserved bacterial second messenger that has been implicated in a wide range of cellular processes. our earlier studies showed that c-di-amp regulates central metabolism in listeria monocytogenes by inhibiting its pyruvate carboxylase (lmpc), a biotin-dependent enzyme with biotin carboxylase (bc) and carboxyltransferase (ct) activities. we report here structural, biochemical, and functional studies on the inhibition of lactococcus ... | 2017 | 28808024 |
| fate of listeria innocua during production and ripening of smeared hard cheese made from raw milk. | the fate of 2 different listeria innocua strains was analyzed during the production and ripening of smeared raw milk greyerzer cheese (gruyère). these strains were used as surrogates for the pathogenic listeria monocytogenes, as they are physiologically very similar. bacterial cells were added to the cheese milk at levels of 10(5) cfu/ml. during the first 24 h of cheese making, the number of the test strains decreased to a level of below 10(2) cfu/g. obviously, the cooking temperature of 56°c an ... | 2017 | 28755934 |
| occurrence and antibiotic susceptibility of listeria species and staphylococcus aureus in cattle slaughterhouses of kerala, south india. | a total of 765 samples were collected from beef carcasses, knives, cutting table surfaces, beef, hands, air, and water from four cattle slaughterhouses of kerala, south india, to determine the occurrence and antibiotic susceptibility of listeria species and staphylococcus aureus. listeria spp. were isolated from beef carcasses (2.0%), knives (3.7%), cutting table surfaces (1.9%), beef (0.7%), and water (1.3%). the identified species were listeria monocytogenes (0.1%), listeria innocua (0.9%), an ... | 2017 | 28742382 |
| stress survival islet 2, predominantly present in listeria monocytogenes strains of sequence type 121, is involved in the alkaline and oxidative stress responses. | the foodborne pathogen listeria monocytogenes is able to survive a variety of stress conditions leading to the colonization of different niches like the food processing environment. this study focuses on the hypervariable genetic hot spot lmo0443 to lmo0449 haboring three inserts: the stress survival islet 1 (ssi-1), the single-gene insert lmof2365_0481, and two homologous genes of the nonpathogenic species listeria innocua: lin0464, coding for a putative transcriptional regulator, and lin0465, ... | 2017 | 28625982 |
| isolation and characterization of listeria species from rodents in natural environments in china. | listeria is ubiquitous in a variety of environments and can be isolated from a wide range of animal hosts. rodents are capable of carrying pathogenic bacteria in their intestines, such as listeria, and can disseminate those pathogens into the natural environment and to where human activity occurs. in this study, we investigated the occurrence and antimicrobial susceptibility of listeria spp. isolated from wild rodents found in natural environments in china. we collected 341 intestinal fecal samp ... | 2017 | 28588285 |
| comparison of listeria monocytogenes recoveries from spiked mung bean sprouts by the enrichment methods of three regulatory agencies. | three selective enrichment methods, the united states food and drug administration's (fda method), the united states department of agriculture food safety inspection service's (usda method), and the en iso 11290-1 standard method, were assessed for their suitability for recovery of listeria monocytogenes from spiked mung bean sprouts. three parameters were evaluated; the enrichment l. monocytogenes population from singly-spiked sprouts, the enrichment l. monocytogenes population from doubly-spik ... | 2017 | 28576371 |
| comparative experimental infection of listeria monocytogenes and listeria ivanovii in bovine trophoblasts. | listeria monocytogenes is a gram-positive, facultative intracellular and invasive bacterium that has tropism to the placenta, and causes fetal morbidity and mortality in several mammalian species. while infection with l. monocytogenes and l. ivanovii are known as important causes of abortion and reproductive failure in cattle, the pathogenesis of maternal-fetal listeriosis in this species is poorly known. this study used the bovine chorioallantoic membrane explant model to investigate the kineti ... | 2017 | 28467447 |
| genomic characterization helps dissecting an outbreak of listeriosis in northern italy. | listeria monocytogenes (lm) is a bacterium widely distributed in nature and able to contaminate food processing environments, including those of dairy products. lm is a primary public health issue, due to the very low infectious dose and the ability to produce severe outcomes, in particular in elderly, newborns, pregnant women and immunocompromised patients. | 2017 | 28856063 |
| corrigendum: growth inhibitory effects of adhatoda vasica and its potential at reducing listeria monocytogenes in chicken meat. | [this corrects the article on p. 1260 in vol. 8, pmid: 28769879.]. | 2017 | 28855897 |
| occurrence and growth of listeria monocytogenes in packaged raw milk. | the increased availability of packaged raw drinking milk necessitates the investigation of the occurrence and growth of listeria monocytogenes in raw milk during distribution and storage. the occurrence of l. monocytogenes in 105 retailed raw milk bottles, 115 bulk tank milk samples, 23 in-line milk filter socks and in 50 environmental samples collected from an on-farm dairy establishment were investigated. growth of inoculated low-level l. monocytogenes contamination was also investigated in tw ... | 2017 | 28850852 |
| multiple cortical brain abscesses due to listeria monocytogenes in an immunocompetent patient. | listeria monocytogenes is an intracellular organism which is well recognised for its ability to cause meningeal infections in neonates, immunosuppressed, debilitated and elderly individuals. (1) other less common central nervous system (cns) infections caused by listeria spp. include rhomboencephalitis, cerebritis and abscesses in the brain, brain stem and spinal cord. the neuroradiological appearance of listeria brain abscesses is similar to other types and may also mimic primary or metastatic ... | 2017 | 28849735 |
| impact of cooking procedures and storage practices at home on consumer exposure to listeria monocytogenes and salmonella due to the consumption of pork meat. | the objective of this research was to analyze the impact of different cooking procedures (i.e., gas hob and traditional static oven) and levels of cooking (i.e., rare, medium, and well-done) on inactivation of listeria monocytogenes and salmonella in pork loin chops. moreover, the consumer's exposure to both microorganisms after simulation of meat leftover storage at home was assessed. the results showed that well-done cooking in a static oven was the only treatment able to inactivate the tested ... | 2017 | 28846810 |
| antimicrobial efficacy of cinnamomum javanicum plant extract against listeria monocytogenes and its application potential with smoked salmon. | extracts from medicinal plants have been reported to possess good antimicrobial properties, but a majority of them remain unexplored. this study aimed at identifying a novel plant extract with antimicrobial activity, to validate its efficacy in food model, and to elucidate its composition and antimicrobial mechanism. a total of 125 plant extracts were screened, and cinnamomum javanicum leaf and stem extract showed potential antimicrobial activity against listeria monocytogenes (mic=0.13mg/ml). t ... | 2017 | 28843123 |
| lactobionic acid enhances the synergistic effect of nisin and thymol against listeria monocytogenes scott a in tryptic soy broth and milk. | listeria monocytogenes is a gram-positive opportunistic human pathogen and it remains a significant cause of foodborne illnesses. a variety of natural and synthetic compounds have been studied to inhibit the growth of l. monocytogenes in foods. antimicrobial combinations with synergistic antilisterial properties can reduce the dose of each antimicrobial, which can be further enhanced by chelating compounds. therefore, the objective of this study was to determine antilisterial properties of binar ... | 2017 | 28843122 |
| the arsenic resistance listeria genomic island lgi2 exhibits sequence and integration site diversity and propensity for three listeria monocytogenes clones with enhanced virulence. | in the foodborne pathogen listeria monocytogenes, arsenic resistance is primarily encountered in serotype 4b clones considered to have enhanced virulence, and is associated with an arsenic resistance gene cluster within a 35 kb chromosomal region, listeria genomic island 2 (lgi2). lgi2 was first identified in strain scott a and includes genes putatively involved in arsenic and cadmium resistance, dna integration, conjugation and pathogenicity. however, genomic localization and sequence content o ... | 2017 | 28842547 |
| spontaneous virulence loss in natural populations of listeria monocytogenes. | listeria monocytogenes (lm) pathogenesis depends on its ability to escape from the phagosome of the host cells via the action of the pore-forming toxin listeriolysin o (llo). expression of the llo-encoding gene (hly) requires the transcriptional activator prfa, and both hly and prfa genes are essential for lm virulence. here we used the hemolytic activity of llo as a phenotypic marker to screen for spontaneous virulence-attenuating mutations in lm sixty (0.1%) non-hemolytic isolates were identif ... | 2017 | 28827366 |
| crystal diagnostics xpress(™) lm kit for the rapid detection of listeria monocytogenes from environmental surfaces. | the crystal diagnostics (cdx) xpress™ lm kit is used for rapid screening of low concentrations of listeria monocytogenes on environmental surfaces such as stainless steel, plastic, and ceramic tile. in addition to the xpress lm kit, the cdx xpress system comprises an automatic xpress reader, a biocassette™ that incorporates antibody-coupled microspheres, and liquid crystal for selective identification of the intended microbe. all 56 of the 56 tested l. monocytogenes strains evaluated were detect ... | 2017 | 28825544 |
| evaluation of 3m molecular detection assay (mda) 2-listeria for the detection of listeria species in select foods and environmental surfaces: collaborative study, first action 2016.07. | 3m molecular detection assay (mda) 2-listeria uses loop-mediated isothermal amplification and bioluminescence detection to rapidly detect listeria species in a broad range of food types and environmental surfaces. using an unpaired study design, mda 2-listeria was compared with the u.s. department of agriculture, food safety and inspection service's microbiology laboratory guidebook chapter 8.09 "isolation and identification of listeria monocytogenes from red meat, poultry and egg products, and ... | 2017 | 28825539 |
| molecular identification of disk abalone (haliotis discus discus) tetraspanin 33 and cd63: insights into potent players in the disk abalone host defense system. | tetraspanins are a superfamily of transmembrane proteins involved in a diverse range of physiological processes including differentiation, adhesion, signal transduction, cell motility, and immune responses. in the present study, two tetraspanins, cd63 and tetraspanin 33 (tspan33) from disk abalone (abcd63 and abtspan33), were identified and characterized at the molecular level. the coding sequences for abcd63 and abtspan33 encoded polypeptides of 234 and 290 amino acids (aa) with predicted molec ... | 2017 | 28823981 |
| prospects and progress of listeria-based cancer vaccines. | the development of an effective therapeutic vaccine to induce cancer-specific immunity remains problematic. recently, a species of intracellular pathogen known as listeria monocytogenes (lm) has been used to transfer dna, rna and proteins into tumour cells as well as elicit an immune response against tumour-specific antigens. areas covered: herein, the authors provide the mechanisms of different listeria monocytogenes strains, which are potential therapeutic cancer vaccine vectors, in addition t ... | 2017 | 28823183 |
| a screen for kinase inhibitors identifies antimicrobial imidazopyridine aminofurazans as specific inhibitors of the listeria monocytogenes pasta kinase prka. | bacterial signaling systems such as protein kinases and quorum sensing have become increasingly attractive targets for the development of novel antimicrobial agents in a time of rising antibiotic resistance. the family of bacterial penicillin-binding-protein and serine/threonine kinase-associated (pasta) kinases is of particular interest due to the role of these kinases in regulating resistance to β-lactam antibiotics. as such, small-molecule kinase inhibitors that target pasta kinases may prove ... | 2017 | 28821610 |
| antimicrobial activity and acetylcholinesterase inhibition by extracts from chromatin modulated fungi. | major health challenges as the increasing number of cases of infections by antibiotic multiresistant microorganisms and cases of alzheimer's disease have led to searching new control drugs. the present study aims to verify a new way of obtaining bioactive extracts from filamentous fungi with potential antimicrobial and acetylcholinesterase inhibitory activities, using epigenetic modulation to promote the expression of genes commonly silenced. for such finality, five filamentous fungal species (t ... | 2017 | 28818332 |
| agonist anti-gitr antibody significantly enhances the therapeutic efficacy of listeria monocytogenes-based immunotherapy. | we previously demonstrated that in addition to generating an antigen-specific immune response, listeria monocytogenes (lm)-based immunotherapy significantly reduces the ratio of regulatory t cells (tregs)/cd4(+) and myeloid-derived suppressor cells (mdscs) in the tumor microenvironment. since lm-based immunotherapy is able to inhibit the immune suppressive environment, we hypothesized that combining this treatment with agonist antibody to a co-stimulatory receptor that would further boost the ef ... | 2017 | 28815044 |
| listeria monocytogenes and shigella flexneri activate the nlrp1b inflammasome. | activation of the innate immune receptor nlrp1b leads to the formation of an inflammasome, which induces autoproteolytic processing of pro-caspase-1, and ultimately to the release of inflammatory cytokines and to the execution of pyroptosis. one of the signals to which nlrp1b responds is metabolic stress that occurs in cells deprived of glucose or treated with metabolic inhibitors. nlrp1b might, therefore, sense microbial infection as intracellular pathogens such as listeria monocytogenes and sh ... | 2017 | 28808162 |
| listeria monocytogenes has both a bd-type and an aa3 -type terminal oxidase which allow growth in different oxygen levels and both are important in infection. | listeria monocytogenes is a food borne pathogen responsible for a number of life-threatening infections of humans. during an infection it invades epithelial cells before spreading from the intestine to the cells of the liver and spleen. this requires an ability to adapt to varying oxygen levels. here we demonstrate that l. monocytogenes has two terminal oxidases, a cytochrome bd-type (cydab) and a cytochrome aa3 -type menaquinol oxidase (qoxab) and that both are used for respiration under differ ... | 2017 | 28808161 |
| agonist anti-gitr antibody significantly enhances the therapeutic efficacy of listeria monocytogenes-based immunotherapy. | we previously demonstrated that in addition to generating an antigen-specific immune response, listeria monocytogenes (lm)-based immunotherapy significantly reduces the ratio of regulatory t cells (tregs)/cd4(+) and myeloid-derived suppressor cells (mdscs) in the tumor microenvironment. since lm-based immunotherapy is able to inhibit the immune suppressive environment, we hypothesized that combining this treatment with agonist antibody to a co-stimulatory receptor that would further boost the ef ... | 2017 | 28807056 |
| thermal inactivation of listeria monocytogenes and salmonella during water and steam blanching of vegetables. | thermal inactivation of listeria monocytogenes and salmonella was evaluated on peas, spinach, broccoli, potatoes, and carrots that were treated with hot water and steam. one gram-positive bacterium, l. monocytogenes, and one gram-negative bacterium, salmonella, were selected as pertinent human pathogens for evaluation. samples were inoculated with a composite of five strains each of l. monocytogenes and salmonella to achieve approximately 10(8) to 10(9) cfu/g. inoculated samples were treated wit ... | 2017 | 28805458 |
| investigating the susceptibility of mice to a bacterial challenge after intravenous exposure to durable nanoparticles. | the goal of this study was to determine whether bacterial clearance in a rodent model would be impaired upon exposure to gold, silver or silica nanoparticles (nps). | 2017 | 28805153 |
| recombinant lcmv vectors induce protective immunity following homologous and heterologous vaccinations. | successful vaccination against cancer and infectious diseases relies on the induction of adaptive immune responses that induce high-titer antibodies or potent cytoxic t cell responses. in contrast to humoral vaccines, the amplification of cellular immune responses is often hampered by anti-vector immunity that either pre-exists or develops after repeated homologous vaccination. replication-defective lymphocytic choriomeningitis virus (lcmv) vectors represent a novel generation of vaccination vec ... | 2017 | 28803862 |
| sequential transition of the injury phenotype, temperature-dependent survival and transcriptional response in listeria monocytogenes following lethal h2o2 exposure. | the food-borne pathogen listeria monocytogenes is present persistently in food processing environments, where this bacterium is exposed to various stress factors, including oxidative stress. this study aimed to elucidate the temperature-dependent response of l. monocytogenes to h2o2 exposure and the phenotypic changes in colony formation by h2o2-treated bacteria. survival curves indicated an increase in the resistance to h2o2 in l. monocytogenes as the temperature decreased during the stress exp ... | 2017 | 28803156 |
| two-step large-volume magnetic separation combined with pcr assay for sensitive detection of listeria monocytogenes in pasteurized milk. | immunomagnetic separation (ims) is an effective tool for the preconcentration and purification of food-borne pathogens from complex food samples because of its high capture efficiency (ce). in conventional ims, antibodies are usually conjugated on the surface of magnetic beads (mb); the random orientation and conformation changes of antibodies on the mb surface can decrease their bioactivity. moreover, the brownian motion of immobilized antibodies is weakened, thereby rendering their binding eff ... | 2017 | 28803008 |
| modeling carbon dioxide effect in a controlled atmosphere and its interactions with temperature and ph on the growth of l. monocytogenes and p. fluorescens. | the effect of carbon dioxide, temperature, and ph on growth of listeria monocytogenes and pseudomonas fluorescens was studied, following a protocol to monitor microbial growth under a constant gas composition. in this way, the co2 dissolution didn't modify the partial pressures in the gas phase. growth curves were acquired at different temperatures (8, 12, 22 and 37 °c), ph (5.5 and 7) and co2 concentration in the gas phase (0, 20, 40, 60, 80, 100% of the atmospheric pressure, and over 1 bar). t ... | 2017 | 28800830 |
| combination of endolysins and high pressure to inactivate listeria monocytogenes. | outbreaks of listeriosis are often related to the consumption of low-processed ready-to-eat food products (e.g. soft cheeses or smoked fish) contaminated with listeria monocytogenes. traditional preservation techniques, such as heat treatment, cannot eliminate listeria from these products without strongly affecting the quality of the foods. we therefore investigated the use of endolysin (plyp40, ply511, or plyp825) in combination with high hydrostatic pressure processing to kill l. monocytogenes ... | 2017 | 28800829 |
| listeria monocytogenes incidence changes and diversity in some brazilian dairy industries and retail products. | listeria monocytogenes can cause listeriosis, a severe foodborne disease. in brazil, despite very few reported cases of listeriosis, the pathogen has been repeatedly isolated from dairies. this has led the government to implement specific legislation to reduce the hazard. here, we determined the incidence of l. monocytogenes in five dairies and retail products in the southeast and midwest regions of brazil over eight months. of 437 samples, three samples (0.7%) from retail and only one sample (0 ... | 2017 | 28800821 |
| mathematical model of interaction between bacteriocin-producing lactic acid bacteria and listeria. part 2: bifurcations and applications. | the big challenge for the food industry is the attending to demands for minimally processed foods, avoiding intense heat treatments and reducing the addition of chemical preservatives, but at the same time ensuring microbiological safety of these products. lactic acid bacteria are traditionally used in the production of fermented foods. they are responsible for the production of antimicrobial compounds, such as organic acids and bacteriocins, which are protein compounds with bactericidal effect ... | 2017 | 28799082 |
| synergy of a combination of nisin and citric acid against staphylococcus aureus and listeria monocytogenes. | food-borne diseases caused by pathogens, such as staphylococcus aureus and listeria monocytogenes, have long attracted attention globally from researchers, food industries, and food safety authorities. nisin (ns) is the only bacteriocin used worldwide as a generally recognised as safe (gras) food preservative, while citric acid (ca) has an unrestricted use in foods since it has gras status. in this study, synergistic interactions of ns combined with ca against s. aureus and l. monocytogenes were ... | 2017 | 28795907 |
| listeria in adults - truly rare or rarely diagnosed in india? | listeria monocytogenes is a facultative anaerobic intracellular gram positive rod causing infection in pregnant women, extremes of age and immune-compromised hosts. in clinical specimens, the organisms may be gram-variable: laboratory misidentification of l. monocytogenes isolates as diphtheroids, streptococci, or enterococci is not uncommon and the isolation of a diphtheroid from blood or csf should always alert the clinician to the possibility that the organism may be l. monocytogenes. the dis ... | 2017 | 28792179 |
| fate of listeria monocytogenes on fresh apples under different storage temperatures. | fresh apples are typically stored for up to 1 year commercially; different apple varieties require different storage temperatures to maintain their quality characteristics. there is sparse information available about listeria monocytogenes survival on fresh apples under various storage temperatures. the objective of this study was to comprehensively evaluate the effect of storage temperature on apple fruit decay and l. monocytogenes survival. unwaxed apple fruits of selected varieties (fuji and ... | 2017 | 28790993 |
| a rare case of meningoencephalitis by listeria monocytogenes in systemic lupus erythematosus: case report and review. | patients with systemic lupus erythematosus (sle) have a high risk of infection. central nervous system infection and neuropsychiatric sle are both major causes of death. it is vital to distinguish between these two conditions to improve prognosis due to the treatment paradigms required for each condition. here, we report one case of meningoencephalitis by listeria monocytogenes (lm) in a patient with sle who presented with fever and developed headache and altered in consciousness in the hospital ... | 2017 | 28785856 |
| critical roles of mtorc1 signaling and metabolic reprogramming for m-csf-mediated myelopoiesis. | myelopoiesis is necessary for the generation of mature myeloid cells during homeostatic turnover and immunological insults; however, the metabolic requirements for this process remain poorly defined. here, we demonstrate that myelopoiesis, including monocyte and macrophage differentiation, requires mechanistic target of rapamycin complex 1 (mtorc1) signaling and anabolic metabolism. loss of mtorc1 impaired myelopoiesis under steady state and dampened innate immune responses against listeria mono ... | 2017 | 28784627 |
| recent advances in understanding listeria monocytogenes infection: the importance of subcellular and physiological context. | the bacterial pathogen listeria monocytogenes ( lm) is the causative agent of listeriosis, a rare but fatal foodborne disease. during infection, lm can traverse several host barriers and enter the cytosol of a variety of cell types. thus, consideration of the extracellular and intracellular niches of lm is critical for understanding the infection process. here, we review advances in our understanding of lm infection and highlight how the interactions between the host and the pathogen are context ... | 2017 | 28781746 |
| progranulin deficiency causes impairment of autophagy and tdp-43 accumulation. | loss-of-function mutations in grn cause frontotemporal dementia (ftd) with transactive response dna-binding protein of 43 kd (tdp-43)-positive inclusions and neuronal ceroid lipofuscinosis (ncl). there are no disease-modifying therapies for either ftd or ncl, in part because of a poor understanding of how mutations in genes such as grn contribute to disease pathogenesis and neurodegeneration. by studying mice lacking progranulin (pgrn), the protein encoded by grn, we discovered multiple lines of ... | 2017 | 28778989 |
| citrus lemon essential oil: chemical composition, antioxidant and antimicrobial activities with its preservative effect against listeria monocytogenes inoculated in minced beef meat. | lemon (citrus limon) is a flowing plant belonging to the rutaceae family. citrus plants constitute one of the main valuable sources of essential oil used in foods and medicinal purposes. | 2017 | 28774297 |
| growth inhibitory effects of adhatoda vasica and its potential at reducing listeria monocytogenes in chicken meat. | the inhibitory effects of adhatoda vasica ethanolic leaf extract (avele) against listeria monocytogenes were examined to assess its potential to preserve minimally processed meat products safely. the total phenolic, flavonoid, and alkaloid levels in avele were 10.09 ± 4.52 mg of gallic acid equivalents (gae)/g, 22.43 ± 1.62 mg of quercetin equivalents/g, and 19.43 ± 3.90 mg/g, respectively. avele (1, 5, 10, or 20%) had considerable antibacterial effects against l. monocytogenes ncim 24563 in ter ... | 2017 | 28769879 |
| multi-level strategy for identifying proteasome-catalyzed spliced epitopes targeted by cd8(+) t cells during bacterial infection. | proteasome-catalyzed peptide splicing (pcps) generates peptides that are presented by mhc class i molecules, but because their identification is challenging, the immunological relevance of spliced peptides remains unclear. here, we developed a reverse immunology-based multi-level approach to identify proteasome-generated spliced epitopes. applying this strategy to a murine listeria monocytogenes infection model, we identified two spliced epitopes within the secreted bacterial phospholipase plcb ... | 2017 | 28768206 |
| listeria monocytogenes in food-processing facilities, food contamination, and human listeriosis: the brazilian scenario. | listeria monocytogenes is a foodborne pathogen that contaminates food-processing environments and persists within biofilms on equipment, utensils, floors, and drains, ultimately reaching final products by cross-contamination. this pathogen grows even under high salt conditions or refrigeration temperatures, remaining viable in various food products until the end of their shelf life. while the estimated incidence of listeriosis is lower than other enteric illnesses, infections caused by l. monocy ... | 2017 | 28767285 |
| characterisation of inla truncation in listeria monocytogenes isolates from farm animals and human cases in the province of quebec. | the introduction of listeria monocytogenes into the food production chain is a concern, with numerous grouped cases of listeriosis associated with milk-derived or pork-derived products have been documented. management of this zoonotic pathogen considers all strains as an equal risk. recently, a new perspective for characterisation of strain virulence was introduced with the discovery of the unaltered sequence of inla as a determinant of strain virulence; this has also been reported as an infrequ ... | 2017 | 28761668 |
| a role for host cell exocytosis in inlb-mediated internalisation of listeria monocytogenes. | the bacterial surface protein inlb mediates internalisation of listeria monocytogenes into human cells through interaction with the host receptor tyrosine kinase, met. inlb-mediated entry requires localised polymerisation of the host actin cytoskeleton. apart from actin polymerisation, roles for other host processes in listeria entry are unknown. here, we demonstrate that exocytosis in the human cell promotes inlb-dependent internalisation. using a probe consisting of vamp3 with an exofacial gre ... | 2017 | 28745416 |
| fasting metabolism modulates the interleukin-12/interleukin-10 cytokine axis. | a crucial role of cell metabolism in immune cell differentiation and function has been recently established. growing evidence indicates that metabolic processes impact both, innate and adaptive immunity. since a down-stream integrator of metabolic alterations, mammalian target of rapamycin (mtor), is responsible for controlling the balance between pro-inflammatory interleukin (il)-12 and anti-inflammatory il-10, we investigated the effect of upstream interference using metabolic modulators on th ... | 2017 | 28742108 |
| antibacterial activity of timsen(®) (n-alkyl dimethyl benzyl ammonium chloride-40%) in scalding and precooling water in poultry slaughterhouses. | the objective of this study was to evaluate the efficacy of a product based on n-alkyl dimethyl benzyl ammonium chloride-40%, marketed as timsen(®), during scalding and precooling of poultry carcasses in slaughterhouses. to this end, three poultry slaughterhouses (a, b and c) were evaluated. the product was added (200 ppm) to the scalding (58 °c) and precooling water (4 °c), and microbiological analyses were performed of the water and the poultry carcasses before and after timsen(®) addition. th ... | 2017 | 28740319 |
| nuclear carbonic anhydrase 6b associates with prmt5 to epigenetically promote il-12 expression in innate response. | interleukin-12 (il-12) is critical for induction of protective immunity against intracellular bacterial infection. however, the mechanisms for efficient induction of il-12 in innate response remain poorly understood. here we report that the b type of carbonic anhydrase 6 (car6-b, which encoded ca-vi b) is essential for host defense against listeria monocytogenes (lm) infection by epigenetically promoting il-12 expression independent of its carbonic anhydrase activity. deficiency of car6-b attenu ... | 2017 | 28739930 |
| listeria monocytogenes in raw milk, milking equipment and dairy workers: molecular characterization and antimicrobial resistance patterns. | the aim of this study was to evaluate the genetic relatedness and patterns of antimicrobial resistance amongst l. monocytogenes isolated from raw milk, milking equipment, and hand swabs from workers in dairy farms. | 2017 | 28739228 |
| an improve protocol for pcr using lm1 and lm2 primers for listeria monocytogenes detection in food matrices. | several studies have observed that a conventional pcr protocol using primers lm1 and lm2 for the identification of gene hlya listeria monocytogenes generates non-specific pcr amplifications and false positives. for this reason in this study, we provide a modified pcr protocol that improves the specificity of the lm1 and lm2 primers. | 2017 | 28735310 |
| early trigeminal nerve involvement in listeria monocytogenes rhombencephalitis: case series and systematic review. | listeria monocytogenes is associated with rhombencephalitis. however, the exact mechanisms of brainstem invasion remains poorly understood. here, we demonstrate clinical and radiological data suggesting that listeria may invade the brainstem via the trigeminal nerve. three females (41, 64 and 70 years) with culture proven l. monocytogenes bacteremia and rhombencephalitis were investigated in the period of 2014-16. t2-weighted and contrast-enhanced t1-weighted mri revealed a cerebellopontine absc ... | 2017 | 28730571 |
| genome sequences of two listeria monocytogenes strains from nectarines associated with listeriosis in 2014. | listeria monocytogenes is an important foodborne pathogen. here, we present the annotated whole genome of listeria monocytogenes strains f14m01297-c2 and f14m01297-c4, isolated from nectarines distributed by a packing facility in california during an investigation of listeriosis associated with stone fruit in 2014. | 2017 | 28729255 |
| antimicrobial effect of black pepper petroleum ether extract for the morphology of listeria monocytogenes and salmonella typhimurium. | this study aimed to evaluate the effects of black pepper petroleum extract (bppe) on pathogenic bacteria. the extraction from black pepper showed intense antimicrobial activity against the gram-positive listeria monocytogenes atcc 19115 and the gram-negative bacteria salmonella typhimurium atcc 14028. the minimum inhibitory concentrations of bppe against l. monocytogenes and s. typhimurium were 0.625 and 1.25 mg/ml, respectively. detection of alkaline phosphatase outside the cell revealed that b ... | 2017 | 28720964 |
| inactivation of heat adapted and chlorine adapted listeria monocytogenes atcc 7644 on tomatoes using sodium dodecyl sulphate, levulinic acid and sodium hypochlorite solution. | the effectiveness of sodium dodecyl sulphate (sds), sodium hypochlorite solution and levulinic acid in reducing the survival of heat adapted and chlorine adapted listeria monocytogenes atcc 7644 was evaluated. the results against heat adapted l. monocytognes revealed that sodium hypochlorite solution was the least effective, achieving log reduction of 2.75, 2.94 and 3.97 log colony forming unit (cfu)/ml for 1, 3 and 5 minutes, respectively. sds was able to achieve 8 log reduction for both heat a ... | 2017 | 28713785 |
| [listeriosis of central nervous system in patients with ulcerative colitis - case study]. | in the recently an increase morbidity inflammatory bowel disease, including ulcerative colitis was observed. the use of purine analogs and their metabolites are associated with a higher incidence of infections in this group of patients. listeriosis is an infectious disease caused by a relatively anaerobic gram-positive bacteria - listeria monocytogenes. common symptoms include fever, nausea, vomiting and diarrhea, but these pathogens can also cause myocarditis, central nervous system infections, ... | 2017 | 28713103 |
| listeria monocytogenes pdee, a phosphodiesterase that contributes to virulence and has hydrolytic activity against cyclic mononucleotides and cyclic dinucleotides. | we have identified and partially characterized a putative hd domain hydrolase, lmof2365_2464, which is highly expressed during listerial intracellular replication. lmof2365_2464 is annotated as a putative hd domain-containing hydrolase. the ability of an isogenic mutant strain, f2365δ2464, to adhere, invade and replicate in intestinal epithelial cells (caco-2) was significantly lower than parent strain f2365. colonization of mouse liver and spleen by l. monocytogenes f2365 was significantly high ... | 2017 | 28711509 |
| variations in biofilm formation, desiccation resistance and benzalkonium chloride susceptibility among listeria monocytogenes strains isolated in canada. | listeria monocytogenes is a pathogenic foodborne microorganism noted for its ability to survive in the environment and food processing facilities. survival may be related to the phenotype of individual strains including the ability to form biofilms and resist desiccation and/or sanitizer exposure. the objectives of this research were to compare 14 l. monocytogenes strains isolated from blood (3), food (6) and water (5) with respect to their benzalkonium chloride (bac) sensitivity, desiccation re ... | 2017 | 28710947 |
| hyperinvasiveness and increased intercellular spread of listeria monocytogenes sequence type 1 are independent of listeriolysin s, internalin f and internalin j1. | listeria monocytogenes is a genetically heterogeneous species, which is divided into evolutionary lineages and clonal complexes (ccs). not all l. monocytogenes isolates are equally likely to cause disease, with cc1, and in particular sequence type (st) 1, being the most prevalent complex in human and ruminant infections and more specifically in neurolisteriosis. while the major factors that determine neurotropism are unknown, the l. monocytogenes cc1 strains harbour listeriolysin s (lls) and par ... | 2017 | 28708050 |
| growth kinetics of listeria monocytogenes in cut produce. | cut produce continues to constitute a significant portion of the fresh fruit and vegetables sold directly to consumers. as such, the safety of these items during storage, handling, and display remains a concern. cut tomatoes, cut leafy greens, and cut melons, which have been studied in relation to their ability to support pathogen growth, have been specifically identified as needing temperature control for safety. data are needed on the growth behavior of foodborne pathogens in other types of cu ... | 2017 | 28708030 |
| sglt1 deficiency turns listeria infection into a lethal disease in mice. | cellular glucose uptake may involve either non-concentrative glucose carriers of the glut family or na+-coupled glucose-carrier sglt1, which accumulates glucose against glucose gradients and may thus accomplish cellular glucose uptake even at dramatically decreased extracellular glucose concentrations. sglt1 is not only expressed in epithelia but as well in tumour cells and immune cells. immune cell functions strongly depend on their metabolism, therefore we hypothesized that deficiency of sglt1 ... | 2017 | 28704812 |
| thioredoxin a is essential for motility and contributes to host infection of listeria monocytogenes via redox interactions. | microbes employ the thioredoxin system to defend against oxidative stress and ensure correct disulfide bonding to maintain protein function. listeria monocytogenes has been shown to encode a putative thioredoxin, trxa, but its biological roles and underlying mechanisms remain unknown. here, we showed that expression of l. monocytogenes trxa is significantly induced in bacteria treated with the thiol-specific oxidizing agent, diamide. deletion of trxa markedly compromised tolerance of the pathoge ... | 2017 | 28702378 |
| strategies for pathogen biocontrol using lactic acid bacteria and their metabolites: a focus on meat ecosystems and industrial environments. | the globalization of trade and lifestyle ensure that the factors responsible for the emergence of diseases are more present than ever. despite biotechnology advancements, meat-based foods are still under scrutiny because of the presence of pathogens, which causes a loss of consumer confidence and consequently a fall in demand. in this context, lactic acid bacteria (lab) as gras organisms offer an alternative for developing pathogen-free foods, particularly avoiding listeria monocytogenes, with m ... | 2017 | 28696370 |
| synergistic antimicrobial combinations inhibit and inactivate listeria monocytogenes in neutral and acidic broth systems. | the use of antimicrobial compounds can be an effective approach to control listeria monocytogenes in ready-to-eat foods, but it can also be limited by cost, restrictions on concentrations in foods, and potential changes to organoleptic properties. combinatorial approaches that produce additive or synergistic effects allow for reductions in individual antimicrobial concentrations while achieving the same level of control. the present study determined the mic and mbc of an antimicrobial compound w ... | 2017 | 28691884 |
| molecular docking based screening of listeriolysin-o for improved inhibitors. | listeriolysine-o (llo) is a 50kda protein responsible for listeria monocytogenes pathogenicity. the structure of llo (pdb id: 4cdb) with domains d1 to d4 is known. therefore, it is of interest to identify conserved regions among llo variants for destabilizing oligomerization (50 mer complex) of its monomers using appropriate inhibitors. therefore, it is of interest to identify suitable inhibitors for inhibiting llo. previous reports suggest the use of flavanoids like compounds for inhibiting llo ... | 2017 | 28690383 |
| fast detection of listeria monocytogenes through a nanohybrid quantum dot complex. | listeria monocytogenes is a recognized foodborne pathogen that causes listeriosis in susceptible consumers. currently, the detection systems for listeria in food detect live and dead bacteria, being the viable microorganisms most relevant for their ability to cause sickness in the population at risk. for this reason, a new nanohybrid compound was developed for the optical detection of listeria that was based on polyamidoamine dendrimers functionalized with an auxotrophic cofactor (lipoic acid), ... | 2017 | 28689324 |
| a cysteine protease (cathepsin z) from disk abalone, haliotis discus discus: genomic characterization and transcriptional profiling during bacterial infections. | cathepsin z (ctsz) is lysosomal cysteine protease of the papain superfamily. it participates in the host immune defense via phagocytosis, signal transduction, cell-cell communication, proliferation, and migration of immune cells such as monocytes, macrophages, and dendritic cells. hence, ctsz is also acknowledged as an acute-phase protein in host immunity. in this study, we sought to identify the ctsz homolog from disk abalone (abctsz) and characterize it at the molecular, genomic, and transcrip ... | 2017 | 28687334 |
| mesoporous tungsten oxides with crystalline framework for highly sensitive and selective detection of foodborne pathogens. | foodborne pathogens like listeria monocytogenes can cause various illnesses and pose a serious threat to public health. they produce species-specific microbial volatile organic compounds, i.e., the biomarkers, making it possible to indirectly measure microbial contamination in foodstuff. herein, highly ordered mesoporous tungsten oxides with high surface areas and tunable pores have been synthesized and used as sensing materials to achieve an exceptionally sensitive and selective detection of tr ... | 2017 | 28683546 |
| septicemia and meningoencephalitis caused by listeria monocytogenes in two neonatal llamas. | listeriosis is a disease of humans and domestic mammals (mainly ruminants) with variable manifestations, primarily encephalitis, septicemia, and abortion. although listeria monocytogenes readily causes illness in ruminants, the prevalence among domestic south american camelids (llamas and alpacas) is low and has not been documented in their wild counterparts, the vicuna and guanaco. we describe herein the clinical signs, autopsy findings, and histopathology of septicemia and suppurative meningoe ... | 2017 | 28677403 |
| survival of a native toxigenic isolate of listeria monocytogenes cfr 1302 during storage of milk-based foods can be a potential cause of health risk. | the ability of a native toxigenic culture of listeria monocytogenes cfr 1302 to survive and elaborate associated toxigenic trait in ice cream and mango pulp-based lactic fermented milk was studied. the culture of l. monocytogenes inoculated at two initial levels of 4.6 and 5.6 log10 cfu/ml almost remained unaltered during storage of the food products. however, in both the milk-based products, a marginal increase in viable population was observed during 2-4 d of storage as against the initial ino ... | 2017 | 28675967 |
| inflammasome and fas-mediated il-1β contributes to th17/th1 cell induction in pathogenic bacterial infection in vivo. | cd4(+) th cells play crucial roles in orchestrating immune responses against pathogenic microbes, after differentiating into effector subsets. recent research has revealed the importance of ifn-γ and il-17 double-producing cd4(+) th cells, termed th17/th1 cells, in the induction of autoimmune and inflammatory diseases. in addition, th17/th1 cells are involved in the regulation of infection caused by the intracellular bacterium mycobacterium tuberculosis in humans. however, the precise mechanism ... | 2017 | 28674179 |
| cd8(+) memory t-cell inflation renders compromised cd4(+) t-cell-dependent cd8(+) t-cell immunity via naïve t-cell anergy. | whether inflation of cd8(+) memory t (mt) cells, which is often derived from repeated prime-boost vaccinations or chronic viral infections in the elderly, would affect late cd8(+) t-cell immunity is a long-standing paradox. we have previously established an animal model with mt-cell inflation by transferring cona-stimulated monoclonal cd8(+) t cells derived from ova-specific t-cell-receptor transgenic oti mice into irradiation-induced lymphopenic b6 mice. in this study, we also established anoth ... | 2017 | 28670575 |
| internalization of listeria monocytogenes in cantaloupes during dump tank washing and hydrocooling. | recent listeriosis outbreaks and recalls associated with cantaloupes urge for studies to understand the mechanisms of cantaloupe contamination by listeria monocytogenes. postharvest practices such as washing and hydrocooling were suggested to facilitate the contamination of fresh fruits by human pathogens. this study assessed the potential of l. monocytogenes internalization into cantaloupes during dump tank washing and immersion-type hydrocooling in water contaminated with l. monocytogenes. the ... | 2017 | 28667935 |
| cleaning and disinfection of biofilms composed of listeria monocytogenes and background microbiota from meat processing surfaces. | surfaces of food processing premises are exposed to regular cleaning and disinfection (c&d) regimes, using biocides that are highly effective against bacteria growing as planktonic cells. however, bacteria growing in surface associated communities (biofilms) are typically more tolerant towards c&d than their individual free cells counterparts, and survival of pathogens such as listeria monocytogenes may be affected by interspecies interactions within biofilms. in this study, pseudomonas and acin ... | 2017 | 28667108 |
| tracking of listeria monocytogenes in meat establishment using whole genome sequencing as a food safety management tool: a proof of concept. | repeated listeria outbreaks particularly associated with ready-to-eat (rte) delicatessen meat products have been reported annually at global level. the most frequent scenario that led to foodborne outbreaks was the post-thermal treatment cross-contamination of deli meat products during slicing and modified atmosphere packaging (map). the precondition for such cross contamination is the previous introduction of listeria into meat processing facilities and subsequent colonization of the production ... | 2017 | 28666130 |
| strand specific rna-sequencing and membrane lipid profiling reveals growth phase-dependent cold stress response mechanisms in listeria monocytogenes. | the human pathogen listeria monocytogenes continues to pose a challenge in the food industry, where it is known to contaminate ready-to-eat foods and grow during refrigerated storage. increased knowledge of the cold-stress response of this pathogen will enhance the ability to control it in the food-supply-chain. this study utilized strand-specific rna sequencing and whole cell fatty acid (fa) profiling to characterize the bacterium's cold stress response. rna and fas were extracted from a cold-t ... | 2017 | 28662112 |