| diviva affects secretion of virulence-related autolysins in listeria monocytogenes. | diviva is a well-conserved coiled-coil protein present in most gram-positive bacteria and has been implicated in division site selection, peptidoglycan biosynthesis and sporulation. diviva proteins bind lipid membranes and characteristically accumulate at curved membrane areas, i.e. the cell poles and the division site, to which they recruit various interaction partners. we have studied the role of this morphogen in the human pathogen listeria monocytogenes and our results suggest a novel mechan ... | 2012 | 22353466 |
| characterization of listeria monocytogenes isolates in import food products of china from 8 provinces between 2005 and 2007. | a total of 48 listeria monocytogenes isolates of different import food products from 8 provinces between 2005 and 2008 were characterized. the serotype and virulence were confirmed for each strain and molecular subtyping were analyzed by multilocus sequence typing (mlst). twenty five strains were assigned to serotype 1/2a, and 11 isolates to serotype 1/2b, serotype 4b were found in 7 isolate, and the remaining 5 strains were grouped into serotypes 1/2c, 4a, and 4e. molecular subtyping schemes fo ... | 2012 | 22352748 |
| effect of lactose monolaurate on pathogenic and nonpathogenic bacteria. | the antimicrobial activities of sucrose monolaurate and a novel ester, lactose monolaurate (lml), were tested. gram-positive bacteria were more susceptible than gram-negative bacteria to both esters. the minimal bactericidal concentrations of lml were 5 to 9.5 mm for listeria monocytogenes isolates and 0.2 to 2 mm for mycobacterium isolates. | 2012 | 22344640 |
| modulation of antibiotic resistance in bacterial pathogens by oleanolic acid and ursolic acid. | antibiotic resistance among bacterial pathogens is a serious problem for human and veterinary medicine, which necessitates the development of novel therapeutics and antimicrobial strategies. some plant-derived compounds, e.g. pentacyclic triterpenoids such as oleanolic acid (oa) and ursolic acid (ua), have potential as a new class of antibacterial agents as they are active against many bacterial species, both gram-positive and gram-negative, and specifically target the cell envelope. the aim of ... | 2012 | 22341643 |
| sequence typing confirms that a predominant listeria monocytogenes clone caused human listeriosis cases and outbreaks in canada from 1988 to 2010. | human listeriosis outbreaks in canada have been predominantly caused by serotype 1/2a isolates with highly similar pulsed-field gel electrophoresis (pfge) patterns. multilocus sequence typing (mlst) and multi-virulence-locus sequence typing (mvlst) each identified a diverse population of listeria monocytogenes isolates, and within that, both methods had congruent subtypes that substantiated a predominant clone (clonal complex 8; virulence type 59; proposed epidemic clone 5 [ecv]) that has been c ... | 2012 | 22337989 |
| the non-coding rna world of the bacterial pathogen listeria monocytogenes. | in the past ten years, listeria monocytogenes has emerged as a model organism in infection biology and also become an attractive system for the study of regulatory rnas in pathogenic bacteria. due to the recent completion of several transcriptomic studies, it is now clear that l. monocytogenes possesses a large repertoire of both cis- and trans-encoded rnas. these include numerous small rnas (srnas) expressed during infection, widespread transcription of both short and long antisense rnas (asrna ... | 2012 | 22336762 |
| [antibiotic resistance and molecular typing of listeria monocytogenes from foods in shandong province from 2009 to 2010]. | to know the antibiotic resistance and molecular characteristics of listeria monocytogenes in shandong province and to study the relationship between antibiotic resistance phenotypes and genome types. | 2011 | 22336337 |
| enumeration of bacteriophage particles: comparative analysis of the traditional plaque assay and real-time qpcr- and nanosight-based assays. | bacteriophages are increasingly being utilized and considered for various practical applications, ranging from decontaminating foods and inanimate surfaces to human therapy; therefore, it is important to determine their concentrations quickly and reliably. traditional plaque assay (pa) is the current "gold standard" for quantitating phage titers. however, it requires at least 18 h before results are obtained, and they may be significantly influenced by various factors. therefore, two alternative ... | 2011 | 22334864 |
| enhanced antimicrobial activity of nisin-loaded liposomal nanoparticles against foodborne pathogens. | this study was designed to evaluate the prolonged antimicrobial stability of nisin-loaded liposome (lipon) nanoparticles against listeria monocytogenes and staphylococcus aureus. the sizes of bare liposomes and lipon were uniformly distributed between 114 and 125 nm. the nanoparticles were homogeneously dispersed in water with less than 0.2 of polydispersity index. the zeta potential value of lipon was +17.1 mv due to the positive charged nisin, attaining 70% of loading efficiency. the minimum i ... | 2012 | 22329855 |
| two novel type ii restriction-modification systems occupying genomically equivalent locations on the chromosomes of listeria monocytogenes strains. | listeria monocytogenes is responsible for the potentially life-threatening food-borne disease listeriosis. one epidemic-associated clonal group of l. monocytogenes, epidemic clone i (eci), harbors a sau3ai-like restriction-modification (rm) system also present in the same genomic region in certain strains of other lineages. in this study, we identified and characterized two other, novel type ii rm systems, lmoj2 and lmoj3, at this same locus. lmoj2 and lmoj3 appeared to recognize gcwgc (w = a or ... | 2012 | 22327591 |
| assessing the contributions of the lias histidine kinase to the innate resistance of listeria monocytogenes to nisin, cephalosporins, and disinfectants. | the listeria monocytogenes liasr two-component system (2cs) encoded by lmo1021 and lmo1022 plays an important role in resistance to the food preservative nisin. a nonpolar deletion in the histidine kinase-encoding component (δlias) resulted in a 4-fold increase in nisin resistance. in contrast, the δlias strain exhibited increased sensitivity to a number of cephalosporin antibiotics (and was also altered with respect to its response to a variety of other antimicrobials, including the active agen ... | 2012 | 22327581 |
| activation of the unfolded protein response by listeria monocytogenes. | the endoplasmic reticulum (er) responds to perturbation of homeostasis with stress. to maintain er function, a signalling-circuitry has evolved which, when engaged, attempts to reduce a surplus of unfolded proteins by triggering the unfolded protein response (upr). several studies have implicated upr in viral infections, neurodegenerative disorders and metabolic diseases but upr has not yet been widely linked to bacterial infections. here we demonstrate that the facultative intracellular pathoge ... | 2012 | 22321539 |
| prevalence, populations and pheno- and genotypic characteristics of listeria monocytogenes isolated from ready-to-eat vegetables marketed in são paulo, brazil. | listeria monocytogenes is a foodborne pathogen of great concern due to the high fatality rates of listeriosis. the consumption of rte vegetables has increased in brazil over the last two decades, but little is known about the risks associated to the consumption of these products. this study evaluated the prevalence and counts of l. monocytogenes in 512 packages of ready-to-eat vegetables marketed in são paulo. the isolates were characterized for their serotypes, ribotypes, positivity for virulen ... | 2012 | 22321293 |
| engagement of heterogeneous nuclear ribonucleoprotein m with listeriolysin o induces type i interferon expression and restricts listeria monocytogenes growth in host cells. | listeriolysin o (llo) is a key virulence factor secreted by the gram-positive, facultative intracellular pathogen listeria monocytogenes (lm). its role in host cell response is still not very clear. using pull-down assay, mass spectrometry analysis and immunoprecipitation approaches, we found that llo interacted with heterogeneous nuclear ribonucleoprotein m (hnrnpm), a member of rna splicing complex apparatus, and the binding domain of llo for hnrnp m was located between amino acids 26 and 176. ... | 2012 | 22317749 |
| genomic presence of gadd1 glutamate decarboxylase correlates with the organization of ascb-dape internalin cluster in listeria monocytogenes. | the ability to survive and proliferate in acidic environments is a prerequisite for the infection of listeria monocytogenes. the glutamate decarboxylase (gad) system is responsible for acid resistance, and three gad homologs have been identified in l. monocytogenes: gadd1, gadd2, and gadd3. to examine whether gad genes are specific to lineage, serovar, or certain subpopulation, we performed a systematic investigation on the prevalence of gad genes in 164 l. monocytogenes. in contrast to gadd2 an ... | 2012 | 22315955 |
| unusual presentation of brain abscess with uncommon organism in an immunocompetent person. | multiple brain abscesses due to listeria monocytogenes was detected in a 55 year old immunocompetent person who had history of progressive weakness for last three months with difficulty in micturition and abnormal behavior for the same duration. the patient was diagnosed six months back as tubercular meningitis and was put on atd (now in continuation phase). after being diagnosed with listeria, the patient was put on antibiotic therapy and responded dramatically. the abscess, by virtue of being ... | 2011 | 22315754 |
| infection of pregnant mice with listeria monocytogenes induces fetal bradycardia. | listeriosis is one of the most lethal bacterial diseases for fetuses and infants. however, pregnant women who get infected with listeria may experience only mild symptoms, making the diagnosis difficult, even when the fetus is fatally infected. | 2012 | 22314663 |
| modelling of bacterial growth with shifts in temperature using automated methods with listeria monocytogenes and pseudomonas aeruginosa as examples. | time to detection (ttd) measurements using turbidometry allow a straightforward method for the measurement of bacterial growth rates under isothermal conditions. growth rate measurements were carried out for listeria monocytogenes at 25, 30 and 37°c and for pseudomonas aeruginosa over the temperature range 25 to 45°c. the classical three-parameter logistic model was rearranged to provide the theoretical foundation for the observed ttd. a model was subsequently developed for the analysis of ttd d ... | 2012 | 22314350 |
| structure-based function discovery of an enzyme for the hydrolysis of phosphorylated sugar lactones. | two enzymes of unknown function from the cog1735 subset of the amidohydrolase superfamily (ahs), lmof2365_2620 (lmo2620) from listeria monocytogenes str. 4b f2365 and bh0225 from bacillus halodurans c-125, were cloned, expressed, and purified to homogeneity. the catalytic functions of these two enzymes were interrogated by an integrated strategy encompassing bioinformatics, computational docking to three-dimensional crystal structures, and library screening. the three-dimensional structure of lm ... | 2012 | 22313111 |
| selective dependence of h2-m3-restricted cd8 responses on il-15. | we studied whether cd8 t cell responses that are mediated by unconventional mhc class ib molecules are il-15 dependent in mice. cd8(+) t cell responses to listeria monocytogenes infection that are restricted by the mhc class ib molecule h2-m3 decreased in the absence of il-15, whereas other primary mhc class ib- and mhc class ia-restricted responses were il-15 independent. this result was confirmed in mhc class ia-deficient mice in which il-15 deficiency also reduced h2-m3-restricted but not all ... | 2012 | 22312130 |
| antimicrobial activity of lauric arginate-coated polylactic acid films against listeria monocytogenes and salmonella typhimurium on cooked sliced ham. | a novel type of environmentally friendly packaging with antibacterial activity was developed from lauric arginate (lae)-coating of polylactic acid (pla) films after surface activation using a corona discharge. scanning electron microscopy (sem)-based analysis of the lae/pla films confirmed the successful coating of lae on the pla surface. the mechanical properties of the lae/pla films with different levels of lae-coating (0% to 2.6%[w/w]) were essentially the same as those of the neat pla film. ... | 2012 | 22309353 |
| listeria monocytogenes shows temperature-dependent and -independent responses to salt stress, including responses that induce cross-protection against other stresses. | the food-borne pathogen listeria monocytogenes experiences osmotic stress in many habitats, including foods and the gastrointestinal tract of the host. during transmission, l. monocytogenes is likely to experience osmotic stress at different temperatures and may adapt to osmotic stress in a temperature-dependent manner. to understand the impact of temperature on the responses this pathogen uses to adapt to osmotic stress, we assessed genome-wide changes in the l. monocytogenes h7858 transcriptom ... | 2012 | 22307309 |
| reduction of listeria monocytogenes in queso fresco cheese by a combination of listericidal and listeriostatic gras antimicrobials. | single and combined effects of three gras (generally recognized as safe) antimicrobials including, bacteriophage p100 (phage p100), lauric arginate (lae), and potassium lactate-sodium diacetate mixture (pl-sd) were evaluated against listeria monocytogenes cold growth in queso fresco cheese (qfc). the fate of phage p100 when exposed to lae (200 ppm) or pl-sd (2.8% pl and 0.2% sd) was determined at 4°c and 30°c in a broth model. phage p100 was found to be stable in the presence of these antimicrob ... | 2012 | 22305889 |
| effect of continuous ohmic heating to inactivate escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in orange juice and tomato juice. | the purpose of this study was to investigate the efficacy of continuous ohmic heating for reducing escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in orange juice and tomato juice. | 2012 | 22292508 |
| cd40-activated b cells can efficiently prime antigen-specific naïve cd8+ t cells to generate effector but not memory t cells. | the identification of the signals that should be provided by antigen-presenting cells (apcs) to induce a cd8(+) t cell response in vivo is essential to improve vaccination strategies using antigen-loaded apcs. although dendritic cells have been extensively studied, the ability of other apc types, such as b cells, to induce a cd8(+) t cell response have not been thoroughly evaluated. | 2012 | 22291907 |
| hyperinduction of host beta interferon by a listeria monocytogenes strain naturally overexpressing the multidrug efflux pump mdrt. | many pathogens regulate or modify their immune-stimulating ligands to avoid detection by their infected hosts. listeria monocytogenes, a facultative intracellular bacterial pathogen, interacts with multiple components of mammalian innate immunity during its infection cycle. during replication within the cytosol of infected cells, l. monocytogenes utilizes two multidrug efflux pumps, mdrm and mdrt, to secrete the small nucleic acid second messenger cyclic-di-amp (c-di-amp). host recognition of c- ... | 2012 | 22290148 |
| effect of storage temperature on survival and growth of foodborne pathogens on whole, damaged, and internally inoculated jalapeños (capsicum annuum var. annuum). | there is a lack of general knowledge regarding the behavior of foodborne pathogenic bacteria associated with jalapeño peppers. the survival and growth behaviors of listeria monocytogenes, escherichia coli o157:h7, and salmonella enterica on the interior and exterior of jalapeño peppers were determined under different storage conditions. jalapeños were inoculated with a five-strain cocktail of l. monocytogenes, e. coli o157:h7, or s. enterica on the intact external surface, injured external surfa ... | 2012 | 22289602 |
| modeling the inactivation of salmonella typhimurium, listeria monocytogenes, and salmonella enteritidis on poultry products exposed to pulsed uv light. | pulsed uv light inactivation of salmonella typhimurium on unpackaged and vacuum-packaged chicken breast, listeria monocytogenes on unpackaged and vacuum-packaged chicken frankfurters, and salmonella enteritidis on shell eggs was explained by log-linear and weibull models using inactivation data from previous studies. this study demonstrated that the survival curves of salmonella typhimurium and l. monocytogenes were nonlinear exhibiting concavity. the weibull model was more successful than the l ... | 2012 | 22289588 |
| survival of salmonella enterica, escherichia coli o157:h7, and listeria monocytogenes on inoculated walnut kernels during storage. | the survival of single strains or cocktails of salmonella, escherichia coli o157:h7, and listeria monocytogenes was evaluated on walnut kernels. kernels were separately inoculated with an aqueous preparation of the pathogens at 3 to 10 log cfu/g, dried for 7 days, and then stored at 23°c for 3 weeks to more than 1 year. a rapid decrease of 1 to greater than 4 log cfu/g was observed as the inoculum dried. in some cases, the time of storage at 23°c did not influence bacterial levels, and in other ... | 2012 | 22289584 |
| occurrence and distribution of listeria species in facilities producing ready-to-eat foods in british columbia, canada. | in british columbia (bc), canada, food processing facilities licensed under provincial authority are not required to sample for listeria monocytogenes in food products or processing environments. in 2009, we conducted a survey of dairy, fish, and meat facilities under bc authority to estimate the prevalence of listeria spp. and l. monocytogenes in ready-to-eat (rte) foods and production environments. from august to october, 250 rte food samples and 258 swabs from the food processing environments ... | 2012 | 22289580 |
| bacteria tune interferon responses by playing with chromatin. | bacterial infections, like their viral counterparts, trigger the onset of innate immune defense mechanisms through the release of cytokines, including interferons (ifns). while type i and ii ifn responses to bacteria have long been explored, type iii ifn response remains poorly addressed. we have recently reported that the pathogen listeria monocytogenes triggers the expression of type i and iii ifn genes in epithelial cells, and is able to fine-tune downstream signaling at the chromatin level. ... | 2016 | 22286704 |
| control of the potential health hazards of smoked fish by gamma irradiation. | this study aims to control the presence of listeria monocytogenes and vibrio parahaemolyticus and the formation of biogenic amines in cold-smoked salmon by gamma irradiation. irradiation at doses of 3 and 1 kgy inactivated 6.59 and 6.05 log cfu/g of l. monocytogenes and v. parahaemolyticus in the inoculated samples, respectively. furthermore, irradiation of the un-inoculated samples significantly decreased their microbial populations of mesophilic aerobic bacteria, anaerobic bacteria, psychrophi ... | 2012 | 22285535 |
| [nosocomial outbreak due to listeria monocytogenes in a neonatal unit]. | description of an outbreak of listeria monocytogenes in a neonatal intensive care unit. | 2012 | 22284133 |
| genome-wide transcriptional profiling of the cell envelope stress response and the role of lisrk and cesrk in listeria monocytogenes. | the gram-positive bacterium listeria monocytogenes is widely distributed in the environment and capable of causing food-borne infections in susceptible individuals. in this study, we investigated the cell envelope stress response in l. monocytogenes. whole-genome transcriptional profiling was performed to investigate the response upon exposure to the cell wall antibiotic cefuroxime. differential expression (at least twofold) of 558 genes was observed, corresponding to 20 % of the l. monocytogene ... | 2012 | 22282521 |
| growth curve prediction from optical density data. | a fundamental aspect of predictive microbiology is the shape of the microbial growth curve and many models are used to fit microbial count data, the modified gompertz and baranyi equation being two of the most widely used. rapid, automated methods such as turbidimetry have been widely used to obtain growth parameters, but do not directly give the microbial growth curve. optical density (od) data can be used to obtain the specific growth rate and if used in conjunction with the known initial inoc ... | 2012 | 22280888 |
| [real-time pcr assay for rapid detection of listeria monocytogenes in simulated milk specimens]. | to establish a real-time pcr assay for the rapid detection of listeria monocytogenes in simulated milk specimens. | 2011 | 22279675 |
| development and evaluation of a multiplex pcr for simultaneous detection of five foodborne pathogens. | to develop a rapid multiplex pcr method for simultaneous detection of five major foodborne pathogens (staphylococcus aureus, listeria monocytogenes, escherichia coli o157:h7, salmonella enteritidis and shigella flexneri, respectively). | 2012 | 22268759 |
| anti-inflammatory effects of nicotinic acid in human monocytes are mediated by gpr109a dependent mechanisms. | nicotinic acid (na) treatment has been associated with benefits in atherosclerosis that are usually attributed to effects on plasma lipoproteins. the na receptor gpr109a is expressed in monocytes and macrophages, suggesting a possible additional role for na in modulating function of these immune cells. we hypothesize that na has the potential to act directly on monocytes to alter mediators of inflammation that may contribute to its antiatherogenic effects in vivo. | 2012 | 22267479 |
| modeling the growth rate and lag time of different strains of salmonella enterica and listeria monocytogenes in ready-to-eat lettuce. | the growth parameters (growth rate, μ and lag time, λ) of three different strains each of salmonella enterica and listeria monocytogenes in minimally processed lettuce (mpl) and their changes as a function of temperature were modeled. mpl were packed under modified atmosphere (5% o₂, 15% co₂ and 80% n₂), stored at 7-30 °c and samples collected at different time intervals were enumerated for s. enterica and l. monocytogenes. growth curves and equations describing the relationship between μ and λ ... | 2012 | 22265311 |
| the effect of high hydrostatic pressure on the microbiological quality and safety of carrot juice during refrigerated storage. | the microbial quality of untreated and pressure-treated carrot juice was compared during storage at 4, 8 and 12 °c. high pressure treatment at 500 mpa and 600 mpa (1 min/20 °c) reduced the total counts by approximately 4 log cfu ml⁻¹ and there was very little growth of the survivors during storage at 4 °c for up to 22 days. total counts increased during storage of pressure-treated juice at 8 °c and 12 °c but took significantly longer to reach maximum levels compared to the untreated juice. the m ... | 2012 | 22265302 |
| mudpit analysis of alkaline tolerance by listeria monocytogenes strains recovered as persistent food factory contaminants. | alkaline solutions are used to clean food production environments but the role of alkaline resistance in persistent food factory contamination by listeria monocytogenes is unknown. we used shotgun proteomics to characterise alkaline adapted l. monocytogenes recovered as persistent and transient food factory contaminants. three unrelated strains were studied including two persistent and a transient food factory contaminant determined using multilocus sequence typing (mlst). the strains were adapt ... | 2012 | 22265300 |
| effect of enterocin as-48 in combination with biocides on planktonic and sessile listeria monocytogenes. | enterocin as-48 was tested on a cocktail of listeria monocytogenes strains in planktonic and sessile states, singly or in combination with biocides benzalkonium chloride, cetrimide, hexadecylpyridinium chloride, didecyldimethylammonium bromide, triclosan, poly-(hexamethylen guanidinium) hydrochloride, chlorhexidine, hexachlorophene, and the commercial sanitizers p3 oxonia and p3 topax 66. combinations of sub-inhibitory bacteriocin concentrations and biocide concentrations 4 to 10-fold lower than ... | 2012 | 22265283 |
| inactivation of biofilm cells of foodborne pathogen by aerosolized sanitizers. | the objective of this study was to determine the effect of aerosolized sanitizers on the inactivation of escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes biofilms. biofilms were formed on a stainless steel and polyvinyl chloride (pvc) coupon by using a mixture of three strains each of three foodborne pathogens. six day old biofilms on stainless steel and pvc coupons were treated with aerosolized sodium hypochlorite (shc; 100 ppm) and peracetic acid (100, 200, and 400 ... | 2012 | 22264420 |
| methods used for the detection and subtyping of listeria monocytogenes. | listeria monocytogenes is an important foodborne pathogen responsible for non-invasive and invasive diseases in the elderly, pregnant women, neonates and immunocompromised populations. this bacterium has many similarities with other non-pathogenic listeria species which makes its detection from food and environmental samples challenging. subtyping of l. monocytogenes strains can prove to be crucial in epidemiological investigations, source tracking contamination from food processing plants and d ... | 2012 | 22261140 |
| bioengineered nisin derivatives with enhanced activity in complex matrices. | nisin a is the best known and most extensively characterized lantibiotic. as it is ribosomally synthesized, bioengineering-based strategies can be used to generate variants. we have previously demonstrated that bioengineering of the hinge region of nisin a can result in the generation of variants with enhanced anti-microbial activity against gram-positive pathogens. here we created a larger bank of hinge variant producers and screened for producers that exhibit enhanced bioactivity as assessed b ... | 2012 | 22260415 |
| [microbiological quality of wheat flour consumed in morocco]. | cereal products (soft and hard wheat) are a basic staple food in the moroccan diet. a total of 60 samples of two types of wheat flours used for human consumption were collected; 30 samples among this collection were obtained from various households using moroccan varieties of wheat produced in traditional flour mills. the rest of the samples were purchased from retail wheat flour sources in the rabat and sale city markets. standard plate counts (spc), total and faecal coliforms, clostridium, sal ... | 2012 | 22260306 |
| [construction and characterization of listeria monocytogenes deltaprfa mutant strains]. | listeria monocytogenes (lm) is an important pathogen that can cause serious listeriosis in humans and animals. the pathogenicity of lm has a close relationship with the prfa protein regulating the expression of virulence genes. therefore, we studied the regulation functions of prfa and its role on lm's virulence. | 2011 | 22260054 |
| pathology in practice. listeria monocytogenes. | | 2012 | 22256841 |
| contribution of a tank-binding kinase 1-interferon (ifn) regulatory factor 7 pathway to ifn-γ-induced gene expression. | signal transducers and activators of transcription (stats) and interferon regulatory factors (irfs) share common target genes. here we show that the irf7 gene is regulated by transcription factors stat1 and irf9 in response to the type ii interferon (ifn) ifn-γ. irf7 cooperated with stat1 and irf1 to stimulate the expression of a subset of ifn-γ-induced stat1 target genes. irf7-mediated control of the gbp2 gene required the presence and basal activity of the s/t kinase tank-binding kinase 1 (tbk ... | 2012 | 22252317 |
| prevalence, characterization, and antimicrobial resistance of listeria monocytogenes isolates from bovine hides and carcasses. | listeria monocytogenes isolates from bovine hides and carcasses (n = 812) were mainly of serogroup 1/2a. all strains were positive for internalin genes. several isolates were resistant to oxacillin (72.2%) or clindamycin (37.0%). these findings indicate that l. monocytogenes of beef origin can be considered a public health concern. | 2012 | 22247138 |
| irhom2 regulation of tace controls tnf-mediated protection against listeria and responses to lps. | innate immune responses are vital for pathogen defense but can result in septic shock when excessive. a key mediator of septic shock is tumor necrosis factor-α (tnfα), which is shed from the plasma membrane after cleavage by the tnfα convertase (tace). we report that the rhomboid family member irhom2 interacted with tace and regulated tnfα shedding. irhom2 was critical for tace maturation and trafficking to the cell surface in hematopoietic cells. gene-targeted irhom2-deficient mice showed reduc ... | 2012 | 22246778 |
| mechanisms and immunological effects of apoptosis caused by listeria monocytogenes. | infection with listeria monocytogenes shows an early stage of lymphocyte apoptosis. this is an obligatory stage the extent of which depends on infective dose. lymphocyte apoptosis occurs early and is rapidly superseded, yet it has a strong biological consequence. the immunological effect of lymphocyte apoptosis following infection is increased susceptibility to l. monocytogenes infection due, in part, to upregulation of il-10 on macrophages and dc. lymphocyte apoptosis is dependent on bacterial ... | 2012 | 22244583 |
| probing cd8 t cell responses with listeria monocytogenes infection. | cd8 t cells play a critical role in the control and eradication of intracellular pathogens. increased understanding of cd8 t cell biology provides insight that can be translated into improved vaccination strategies. the intracellular bacterium, listeria monocytogenes, has been used as a model organism to study every phase of the cd8 t cell response to intracellular bacterial infection. infection of laboratory mice with l. monocytogenes has provided insight into the factors that are involved in p ... | 2012 | 22244579 |
| dendritic cells in listeria monocytogenes infection. | dendritic cells (dcs) represent a unique collection of innate immune cells present throughout the body as distinct subpopulations generally sharing the functions of pathogen recognition, cytokine production, and antigen presentation. a large body of work in recent years has examined dc functions during infection with listeria monocytogenes (lm), particularly in the murine model. here, i review several aspects of dc biology in this model, with particular emphasis on the role dcs play in the estab ... | 2012 | 22244578 |
| interactions of listeria monocytogenes with the autophagy system of host cells. | macrophages are immune cells that participate in the host defense against bacterial pathogens. these cells mediate bacterial clearance by internalizing bacteria into a phagosome, which ultimately fuses with lysosomes to kill bacteria. one bacterial strategy to evade killing in the phagosome is to escape from this compartment prior to lysosomal fusion. listeria monocytogenes is a classic example of a "cytosol-adapted pathogen" in that it can rapidly escape from the phagosome in macrophages (and o ... | 2012 | 22244576 |
| studies with listeria monocytogenes lead the way. | | 2012 | 22244575 |
| the prolyl isomerase pin1 modulates development of cd8+ cdc in mice. | pin1 has previously been described to regulate cells that participate in both innate and adaptive immunity. thus far, however, no role for pin1 has been described in modulating conventional dendritic cells, innate antigen presenting cells that potently activate naïve t cells, thereby bridging innate and adaptive immune responses. | 2012 | 22238658 |
| widespread listeriosis outbreak attributable to pasteurized cheese, which led to extensive cross-contamination affecting cheese retailers, quebec, canada, 2008. | a major listeria monocytogenes outbreak occurred in the province of quebec, canada, in 2008, involving a strain of l. monocytogenes (lm p93) characterized by pulsed-field gel electrophoresis (pfge) and associated with the consumption of pasteurized milk cheese. this report describes the results of the ensuing investigation. all individuals affected with lm p93 across the province were interviewed with a standardized questionnaire. microbiological and environmental investigations were conducted b ... | 2012 | 22221357 |
| salt stress-induced transcription of σb- and ctsr-regulated genes in persistent and non-persistent listeria monocytogenes strains from food processing plants. | listeria monocytogenes is a foodborne pathogen that can persist in food processing environments. six persistent and six non-persistent strains from fish processing plants and one persistent strain from a meat plant were selected to determine if expression of genes in the regulons of two stress response regulators, σ(b) and ctsr, under salt stress conditions is associated with the ability of l. monocytogenes to persist in food processing environments. subtype data were also used to categorize the ... | 2012 | 22216988 |
| immunomodulatory effects of danshen (salvia miltiorrhiza) in balb/c mice. | danshen, the root and rhizome of salvia miltiorrhiza bge, a traditional chinese medicine, especially for cardiovascular and cerebrovascular diseases, has unique immunomodulatory effects. danshen is capable of anti-inflammation and antiallergy, which are immunosuppressive activities, whereas it is also able to promote immunity against cancer, viruses, and bacteria. most previous reports were performed with use of a purified compound or compounds of danshen. since there are more than twenty active ... | 2012 | 24049654 |
| neonatal listeriosis: the importance of placenta histological examination-a case report. | neonatal listeriosis is not a rare disease, but it is infrequently diagnosed and reported in the literature. herein we report a case of listeria lethal sepsis, followed by increased cytokines levels in the cord blood, in which diagnosis was made possible by histological examination of the placenta. | 2011 | 23705075 |
| development of a murine model with optimal routes for bacterial infection and treatment, as determined with bioluminescent imaging in c57bl/6 mice. | mice intragastrically infected with listeria monocytogenes egde and staphylococcus aureus xen 36 showed no visible signs of infection over 48 h. however, high numbers (6.2 × 10(5) cfu/mg feces) of s. aureus xen 36 were detected 4 h, and 3.3 × 10(5) cfu/mg feces of l. monocytogenes egde 8 h, after administration. mice intraperitoneally infected with s. aureus xen 36 (1 × 10(7) cfu) developed infection immediately after administration and for at least the following 48 h. injection with higher cell ... | 2011 | 26781578 |
| biopreservation of sardinella longiceps and penaeus monodon using protective culture streptococcus phocae pi 80 isolated from marine shrimp penaeus indicus. | the influence of streptococcus phocae pi80 on the shelf life of sardinella longiceps and penaeus monodon was investigated by measurement of microbial and chemical analysis after appraising the safety of the protective probiotic culture in wistar rat's model. the results of this safety assessment indicate that oral administration of protective culture does not demonstrate any toxicological effects. consumption of this lab strain had no adverse effects on animal's general health status, hematology ... | 2011 | 26781574 |
| [measuring the control and decrease in prevalence of listeria monocytogenes species in foods of animal origin]. | a large distributed bacterium, listeria monocytogenes has been isolated from water and fresh vegetables, raw meat and processed meat (all types), and raw, salted and smoked fish. l. monocytogenes grows at low oxygen concentrations and at low temperatures, surviving for a long time in the environment, in the processing plant, as well as on the equipment, instruments and during storage at the refrigeration temperature. l. monocytogenes causes invasive listeriosis, often affecting immunocompromised ... | 2014 | 23745223 |
| prevalence and characterization of listeria species in domestic and industrial cheeses of isfahan region. | listeria monocytogenes is of major concern to the food industry in general and the dairy industry in particular. little is known about incidence of this pathogenic bacterium in dairy products in iran. | 2011 | 23113091 |
| fate of listeria monocytogenes and members of the enterobacteriaceae group along the processing line of 'wara' a southwestern nigeria soft cheese. | this study aimed at evaluating the survivability of listeria monocytogenes and members of the enterobacteriaceae group of organisms during the processing and storage of wara, a southwestern nigerian un-ripened soft cheese. pasteurized milk inoculated with l. monocytogenes at 10(1) or 10(2) cfu/ml was used to process the cheese. samples were taken along the processing steps and throughout a five-day storage period at 15 and 28 degrees c for populations of l. monocytogenes and enterobacteriaceae. ... | 2010 | 22416662 |
| isolation and confirmation of listeria species from seafood off goa region by polymerase chain reaction. | several food borne outbreaks have highlighted the importance of listeria monocytogenes to the public health and have been recognized as an emerging, important food borne pathogen, and a causative agent of listerioses. a number of genes are involved in the manifestation of listeria virulence, hlya is one among them. in the present study, 111 marine fish samples including prawns, finfishes and bivalves were screened for the presence of listeria species. the isolates were characterized biochemicall ... | 2010 | 22282604 |
| endocarditis due to listeria monocytogenes in an academic teaching hospital: case report. | | 2010 | 22478994 |
| biofilm formation by listeria monocytogenes on stainless steel surface and biotransfer potential. | an experimental model was proposed to study biofilm formation by listeria monocytogenes atcc 19117 on aisi 304 (#4) stainless steel surface and biotransfer potential during this process. in this model, biofilm formation was conducted on the surface of stainless steel coupons, set on a stainless steel base with 4 divisions, each one supporting 21 coupons. trypic soy broth was used as bacterial growth substrate, with incubation at 37 °c and stirring of 50 rpm. the number of adhered cells was deter ... | 2010 | 24031469 |
| listeria monocytogenes' step-like response to sub-lethal concentrations of nisin. | microbial safety of food products is often accomplished by the formulation of food-grade preservatives into the product. because of the growing consumer demand for natural substances (including preservatives) in the composition of consumed foods, there is also a growing interest in the natural antimicrobial nisin, which has generally recognized as safe (gras) status for certain applications. during the products storage time, concentrations of preservative(s) are decreasing, which may eventually ... | 2009 | 26783172 |
| suppression of listeria monocytogenes scott a in fluid milk by free and liposome-entrapped nisin. | nisin is an antimicrobial polypeptide inhibitory toward gram-positive bacterial pathogens, including listeria monocytogenes. encapsulating nisin in lipid nanocapsules (i.e., liposomes) has been shown to protect antimicrobial functionality in complex food matrices. the capacity of liposomes to encapsulate a fluorescent reporter was determined via spectroscopy. survival and growth of l. monocytogenes incubated in fluid milk containing 50 iu/ml free or liposome-entrapped nisin was assayed via perio ... | 2009 | 26783171 |
| the acid tolerance response alters membrane fluidity and induces nisin resistance in listeria monocytogenes. | the ability of l. monocytogenes cells to adapt to a variety of stressors contributes to its growth in a wide range of foods. the present study examines the effect of acid and of the acid tolerance response (atr) on membrane fluidity and on the organism's resistance to acid and to the bacteriocin nisin. when atr was induced in wild-type cells, these cells also became resistant to nisin. atr(+) cells also had lower membrane rigidities than control atr(-) cells that had not been subjected to the ac ... | 2009 | 26783167 |
| proinflammatory and phagocytic functions of alveolar macrophages in obesity. | | 2009 | 24973149 |
| potential of chitosan from mucor rouxxi ucp064 as alternative natural compound to inhibit listeria monocytogenes. | listeria monocytogenes is widely distributed in nature and the infection listeriosis is recognized as a potential threat for human health because of its mortality rate. the objective of this study was to evaluate the growth profile and chitosan production by mucor rouxxi ucp 064 grown in yam bean (pachyrhizus erosus l. urban) medium. it was also to assess the anti-l. monocytogenes efficacy of the obtained chitosan. higher values of biomass of m. rouxxi (16.9 g.l(-1)) and best yield of chitosan ( ... | 2009 | 24031403 |
| characterization of listeria monocytogenes isolated from a fresh mixed sausage processing line in pelotas-rs by pfge. | listeria monocytogenes is a bacterium capable to adhere to the surfaces of equipment and utensils and subsequently form biofilms. it can to persist in the food processing environmental for extended periods of time being able to contaminate the final product. the aim of this study was to trace the contamination route of l. monocytogenes on a fresh mixed sausage processing line, from raw material to the final product. the isolates obtained were characterized by serotyping and molecular typing by p ... | 2009 | 24031402 |
| antiangiogenesis immunotherapy induces epitope spreading to her-2/neu resulting in breast tumor immunoediting. | targeting tumors using cancer vaccine therapeutics has several advantages including the induction of long-term immunity, prime boost strategies for additional treatments and reduced side effects compared to conventional chemotherapeutics. however, one problem in targeting tumor antigens directly is that this can lead to antigen loss or immunoediting. we hypothesized that directing the immune response to a normal cell type required for tumor growth and survival could provide a more stable immunot ... | 2009 | 24367160 |
| prediction of proteins putatively involved in the thiol: disulfide redox metabolism of a bacterium (listeria): the cxxc motif as query sequence. | thiol:disulfide redox metabolism (tdrm) is a central metabolic network in all living cells. however, numerous proteins with different biochemical functions and several structural domains are involved, making it not trivial to identify and annotate its constituents in sequenced genomes. we developed an uncomplicated approach to solve the problem using existing web-based tools and public databases with the gram-positive bacterium listeria monocytogenes egd-e as a model organism. a pattern search f ... | 2009 | 22430441 |
| synergistic effect of nisin and garlic shoot juice against listeria monocytogenes in milk. | the aim of this research was to determine the synergistic effect of nisin and garlic shoot juice (gsj) against listeria monocytogenes atcc 19118 found in whole (3.5%), low (1%) and skim (no fat content) milk. garlic shoot juice (gsj) at concentrations of 2.5%, 5% and 10% revealed strong and similar patterns of antilisterial effect against l. monocytogenes atcc 19118 in all categories of milk. nisin only at concentrations of 62.5, 125, 250 and 500iu/ml displayed a strong antilisterial effect as c ... | 2008 | 26049229 |
| quantitative evaluation of listeria monocytogenes in fresh and processed surubim fish (pseudoplatystoma sp). | l. monocytogenes is a foodborne psychrotrophic bacterial pathogen of special importance for minimally processed foods. in this work, it was enumerated in samples of surubim fish by mpn technique. the population of l. monocytogenes was estimated as < 0.012 mpn/cm(2) in fresh and < 0.03 mpn/g in minimally processed fish. | 2008 | 24031260 |
| behaviour of l. monocytogenes in sliced, vacuum-packed mortadella. | this study evaluated the growth of naturally occurring l. monocytogenes in sliced, vacuum-packed mortadella samples during storage at 5°c until the expiration date. tukey's test indicated that counts of l. monocytogenes on 0, 10, 20, 30 and 40 days of storage were significantly different (p<0.05), indicating growth during shelf life. in three trials, the mean increase was 1.72 log cycles. vacuum packing and storage under refrigeration were not effective in controlling the growth of l. monocytoge ... | 2008 | 24031257 |
| epidemiological survey of listeria monocytogenes in a gravlax salmon processing line. | listeria monocytogenes is a cause of concern to food industries, mainly for those producing ready-to-eat (rte) products. this microorganism can survive processing steps such as curing and cold smoking and is capable of growing under refrigeration temperatures. its presence in rte fish products with extended shelf life may be a risk to the susceptible population. one example of such a product is gravlax salmon; a refrigerated fish product not exposed to listericidal processes and was the subject ... | 2008 | 24031233 |
| molecular analysis of the iap gene of listeria monocytogenes isolated from cheeses in rio grande do sul, brazil. | the polymorphic region sequences in the iap gene were analyzed in 25 strains of listeria monocytogenes isolated from cheeses in the state of rio grande do sul, and compared with reference strains. this investigation distinguished two clusters of l. monocytogenes: i (20 strains) and ii (5 strains). | 2008 | 24031198 |
| host resistance model to an intracellular pathogen. | this unit describes a model for the determination of host resistance in mice using a gram-positive intracellular bacterium, listeria monocytogenes (lm), and discusses its broad use in immunotoxicological studies. the provides detailed procedures for lm infection and sample analysis, including preparation and infection of mice with lm, recording of sickness behaviors in infected mice, and determination of viable lm numbers in the tissues from infected animals. the protocol also describes use of s ... | 2006 | 23045129 |
| listeria monocytogenes and inflammatory bowel diseasedetection of listeria species in intestinal mucosal biopsies by real-time pcr. | | 2003 | 28248597 |
| clinical presentation of acute bacterial meningitis in qatar. | to study the changes in the epidemiology of bacterial meningitis in the era of the hemophilus influenzae (h. influenzae) type b vaccine and pneumoccous resistance. | 2002 | 23978859 |
| cerebellar abscess due to listeria monocytogenes. | brain abscess due to listeria monocytogenes mainly involves the cerebral hemispheres. cerebellar abscess is an infrequent event, which could lead to rapid neurological deterioration if unrecognized. we present a case of multiple brain stem and cerebellar abscesses in a previously healthy individual exposed to unpasteurized milk. this is the 2nd case of cerebellar abscess due to listeria monocytogenes reported in the english literature. the diagnosis of listeria monocytogenes was made in the surg ... | 2002 | 23978925 |
| plasmid-mediated streptomycin resistance of listeria monocytogenes. | a strain of streptomycin-resistant listeria monocytogenes lm35 isolated from imported frozen beef was examined in this study. in conjugation studies, the l. monocytogenes lm35 strain harbouring two plasmids of 54, 3.0, 2.8 and 2.7 kilobase was used as the donor and streptomycin-sensitive and plasmidless l. monocytogenes lm65 and lm100 strains as the recipients. streptomycin resistance was transferred to l. monocytogenes lm65 and lm100 strains at frequencies of 3.3 × 10(-8) and 1.2 × 10(-9) per i ... | 2001 | 22973158 |
| listeria monocytogenes-induced endogenous endophthalmitis in an otherwise healthy. | purpose listeria monocytogenes is a rare cause of endogenous endophthalmitis. to date 15 cases have been published in the literature. all eyes showed similar clinical features and profound visual loss mainly due to delayed diagnosis. | 2017 | 28226136 |
| rhomboencephalitis caused by listeria monocytogenes. | listerial rhomboencephalitis is a rare, devastating treatable disease. a previously well 72-year-old man presented with a one-week history of fever and headache. four days before admission he developed right facial numbness and ataxia. diagnosis and treatment are described, including the relevant clinical, laboratory and radiological findings. | 1997 | 22514485 |
| [not available]. | to review the epidemiology, risk factors, clinical features and outcomes of listeria monocytogenes infection in the eastern townships of quebec, a retrospective study was completed over the period 1976 to 1995. cases were identified from positive cultures and diagnosis given by the infectious diseases service of the centre hospitalier universitaire de sherbrooke, sherbrooke, quebec. a total of 12 cases were in the study. septicemia not related to pregnancy (four of 12) and meningitis (six of 12) ... | 1997 | 22514474 |
| bacteriocins for control of listeria spp. in food. | foodborne outbreaks of listeriosis caused by listeria monocytogenes have contributed to public consciousness about bacterial pathogens involved with foodborne disease. major concerns with l. monocytogenes are its high mortality rate, wide distribution on raw products, growth at low temperatures, and its ability to establish itself in various food-processing environments. these concerns have prompted the examination of novel approaches, including the use of antimicrobial peptides, or bacteriocins ... | 1996 | 28384026 |
| bacterial peritonitis caused by listeria monocytogenes: case report and review of the literature. | although listeria monocytogenes has been isolated from the gastrointestinal tract, it is an infrequent cause of bacterial peritonitis. since 1963 only 23 cases of peritonitis caused by listeria have been reported. this report describes another case in a patient with cirrhosis and chronic renal failure and presents a review of the literature. most (16) of the previous cases were cirrhotic while six were undergoing chronic ambulatory peritoneal dialysis. eight patients were on immunosuppressive th ... | 1996 | 22514418 |
| endogenous endophthalmitis due to listeria monocytogenes. | the authors report a case of listeria monocytogenes endophthalmitis in a patient who had undergone cardiac revascularization surgery and was receiving systemic corticosteroids. the organism was isolated both in the aqueous humor and in blood culture. the infection was successfully treated with intravenous crystalline penicillin, but the patient developed a bullous keratopathy and secondary glaucoma, resulting in poor visual acuity (counting fingers at 0.5 m). the visual acuity recovered to 20/30 ... | 1994 | 22823015 |
| manufacturers have effect on listeriae. | changes in food manufacturing and/or health education measures may have had an impact on the epidemiology of infections with listeria monocytogenes, researchers from leicester say. | 1993 | 27665816 |
| prevention of foodborne listeriosis. | listeria monocytogenes is a gram-positive, rod-shaped bacterium which, although recognized in the medical literature as an opportunistic pathogen for the past 60 years, has only recently gained prominence as an important foodborne pathogen. factors which make this organism unique among foodborne pathogens include its ability both to survive in foods under a variety of adverse conditions and to grow at low refrigeration temperatures. the organism is very widespread in the environment and can be f ... | 1991 | 22529720 |
| what do we do about listeria monocytogenes? | | 1991 | 22529716 |
| salad poisoning. | prepackaged salads may be a source of listeria monocytogenes food poisoning. | 1988 | 27413950 |
| are inflammatory phagocytes responsible for resistance to facultative intracellular bacteria? | at least 38 deaths from listeriosis in the united states in recent months have drawn attention to how little we know about resistance to facultative intracellular bacteria. much more is known about resistance to obligate intracellular parasites. macrophages, activated by t cell-derived macrophage activating factors (maf), are able to kill obligate intracellular parasites and tumor cells(1-10) including leishmania, certain trypanosomes, toxoplasma, the obligate intracellular bacterium rickettsia, ... | 1986 | 25289573 |
| activation or suppression of bactericidal activity of macrophages during a graft-versus-host reaction against i-a and i-j-region differences, respectively. | systemic graft-versus-host reactions (gvhr) were induced in f1 heterozygous mice by injecting 10(8) parental lymphocytes. the anti-thy 1.2-sensitive, t-cell mediated activation of macrophages was assessed by their increased capacity to destroy a facultative intracellular bacterium listeria monocytogenes. the difference in mhc regions causing a gvhr that induced high levels of macrophage activation mapped to i-a. in contrast, differences at k or d, in any of the other h-2 subregions or in the non ... | 1980 | 22457929 |
| microbiological aspects of listeria monocytogenes with special reference to listeriosis in animals. | | 1961 | 24546612 |