| coselection of cadmium and benzalkonium chloride resistance in conjugative transfers from nonpathogenic listeria spp. to other listeriae. | resistance to the quaternary ammonium disinfectant benzalkonium chloride (bc) may be an important contributor to the ability of listeria spp. to persist in the processing plant environment. although a plasmid-borne disinfectant resistance cassette (bcrabc) has been identified in listeria monocytogenes, horizontal transfer of these genes has not been characterized. nonpathogenic listeria spp. such as l. innocua and l. welshimeri are more common than l. monocytogenes in food processing environment ... | 2012 | 22904051 |
| prophage excision activates listeria competence genes that promote phagosomal escape and virulence. | the dna uptake competence (com) system of the intracellular bacterial pathogen listeria monocytogenes is considered nonfunctional. there are no known conditions for dna transformation, and the com master activator gene, comk, is interrupted by a temperate prophage. here, we show that the l. monocytogenes com system is required during infection to promote bacterial escape from macrophage phagosomes in a manner that is independent of dna uptake. further, we find that regulation of the com system r ... | 2012 | 22901809 |
| microbial quality and safety of x-ray irradiated fresh catfish (ictalurus punctatus) fillets stored under co2 atmosphere. | the effect of low-dose x-ray irradiation (0, 2, and 3 kgy) on pathogens, spoilage bacteria (psychrotrophic, anaerobic, and lactic acid bacteria), and physicochemical parameters of iced catfish fillets stored under carbon dioxide (co(2) ) atmosphere were studied. irradiation of 2 or 3 kgy eliminated all listeria monocytogenes (4.8 log cfu/g) and salmonella typhimurium (4.7 log cfu/g) while co(2) atmosphere alone showed less than 1 log reduction on salmonella typhimurium. there was no recovery of ... | 2012 | 22901245 |
| reduction of listeria monocytogenes in raw catfish fillets by essential oils and phenolic constituent carvacrol. | the antimicrobial activity of various essential oils and carvacrol was determined on fresh raw catfish fillets against a 4-strain listeria monocytogenes mixture representing serotypes 1/2b, 3b, 4b, and 4c that were predominantly isolated from catfish processing environments. thyme oil, oregano oil and carvacrol exhibited concentration and time dependent responses in broth against l. monocytogenes; for example 0.5% concentrations resulted in 4 log cfu/ml reduction within 30 min whereas 0.1% conce ... | 2012 | 22901012 |
| occurrence and antimicrobial susceptibility of listeria monocytogenes isolated from brined white cheese in jordan. | listeria monocytogenes is a serious foodborne pathogen that has been isolated from different dairy food products. several foodborne outbreaks of listeriosis have been associated with consumption of cheese. the aims of this study were to determine the occurrence of l. monocytogenes and listeria spp. in brined white cheese (bwc) sold in jordan, and to determine the susceptibility of isolated l. monocytogenes to antimicrobials. three hundred and fifty samples of 5 different types of bwc (akkawi, bo ... | 2012 | 22897495 |
| trophoblastic cell lines ach1p and ac-1m32 react with a distinctive cytokine pattern toward listeria monocytogenes and show morphologic differences. | the differential reaction of cytotrophoblast and syncytiotrophoblast towards infection with listeria monocytogenes (lm) is pivotal to its pathogenicity. in this study we tested the cytokine signature upon infection with listeria monocytogenes (lm) in an in vitro model. | 2012 | 22892046 |
| antimicrobial effects of sodium metasilicate against listeria monocytogenes. | sodium metasilicate (sms) is a u.s. department of agriculture-approved antimicrobial for use in meat and poultry processing and has been known to be effective against various foodborne pathogens. however, its antimicrobial mechanism has not yet been revealed. in this study, we attempted to elucidate the mechanism by which sms inactivates listeria monocytogenes, a gram-positive bacterial pathogen encountered commonly in ready-to-eat meat and poultry products. l. monocytogenes (scott a) cells were ... | 2012 | 22889042 |
| engineered variants of inlb with an additional leucine-rich repeat discriminate between physiologically relevant and packing contacts in crystal structures of the inlb:met complex. | the physiological relevance of contacts in crystal lattices often remains elusive. this was also the case for the complex between the invasion protein internalin b (inlb) from listeria monocytogenes and its host cell receptor, the human receptor tyrosine kinase (rtk) met. inlb is a met agonist and induces bacterial host cell invasion. activation of rtks generally involves ligand-induced dimerization of the receptor ectodomain. the two currently available crystal structures of the inlb:met comple ... | 2012 | 22887347 |
| inhibitory effect of live-attenuated listeria monocytogenes-based vaccines expressing mia gene on malignant melanoma. | listeria monocytogenes (lm), a gram-positive facultative intracellular bacterium, can be used as an effective exogenous antigen expression vector in tumor-target therapy. but for successful clinical application, it is necessary to construct attenuated lm stain that is safe yet retains the potency of lm based on the full virulent pathogen. in this study, attenuated lm and recombinants of lm expressing melanoma inhibitory activity (mia) were constructed successfully. the median lethal dose (ld(50) ... | 2012 | 22886976 |
| enhancement of plasmid dna immunogenicity with linear polyethylenimine. | the utility of plasmid dna as an immunogen has been limited by its weak immunogenicity. in the present study, we evaluated the ability of a family of linear polyethylenimine (pei) polymers, complexed to plasmid dna, to augment dna expression in vivo and to enhance antigen-specific adaptive immune responses. we showed that four of five structurally different peis that we evaluated increased in vivo dna expression 20- to 400-fold, and enhanced dna-induced epitope-specific cd8⁺ t-cell responses 10- ... | 2012 | 22886924 |
| antibacterial metabolites synthesized by psychrotrophic bacteria isolated from cold-freshwater environments. | the ability of three psychrotrophic gram-negative bacilli isolated from chilean patagonian cold freshwater rivers to produce bioactive metabolites was evaluated. the strains were isolated from cold waters rivers and identified by their biochemical properties and 16s rrna gene analysis. the metabolites fractions showing antibacterial activity were obtained by solvent extraction and partially characterized by gas-mass chromatography (gc-ms). antibacterial activity of the fractions was evaluated by ... | 2013 | 22886611 |
| impact of curd milling on the chemical, functional, and rheological properties of starter-free queso fresco. | the manufacture of queso fresco (qf), a high-moisture fresh mexican cheese that is popular in the americas, varies from country to country, with many manufacturers milling the curd before forming the cheese block to disrupt the protein matrix and ensure the crumbly nature of the qf. because this traditional milling step does take time and may be an unnecessary point of microbial contamination, this study was undertaken to determine whether the curd-milling step could be omitted without altering ... | 2012 | 22884346 |
| high level of heterogeneity among listeria monocytogenes isolates from clinical and food origin specimens in greece. | in order to examine the genetic variation of clinical and food isolates of listeria monocytogenes in greece, a total of 61 l. monocytogenes non-duplicate isolates, recovered from clinical specimens (n=19) and food (n=42), were serotyped and genotyped using two different random amplification of polymorphic dna (rapd) protocols and multiple locus variable number tandem repeat analysis (mlva). serotype group 4b, 4d, 4e prevailed (39.4%), among both clinical and food isolates, followed by serotype g ... | 2012 | 22880958 |
| cutting edge: generation of memory precursors and functional memory cd8+ t cells depends on t cell factor-1 and lymphoid enhancer-binding factor-1. | t cell factor (tcf)-1 and lymphoid enhancer-binding factor (lef)-1 transcription factors have redundant roles in promoting thymocyte maturation. tcf-1 has been recently shown to critically regulate memory cd8+ t cell differentiation and persistence. the complete spectra of regulatory roles for tcf-1 and lef-1 in cd8+ t cell responses are yet unknown. we conditionally targeted lef-1, and by combination with germline deletion of tcf-1, we found that loss of both factors completely abrogated the ge ... | 2012 | 22875805 |
| antimicrobial susceptibilities of listeria monocytogenes strains isolated from food and food processing environment in poland. | a total of 471 listeria monocytogenes isolates from different types of food and food-related sources in poland during 2004-2010 were examined. this number includes 200 isolates from fish, 144 from fresh and frozen vegetables, 43 ready-to-eat products (deli foods, cold cuts), 13 from dairy products, 16 from raw meats, 15 from confectionery products and 40 directly from processing plants. all isolates were subjected to serotyping and lineage assays using pcr, and antimicrobial susceptibility using ... | 2012 | 22874767 |
| an unusual form of listerial cns infection. | listeria monocytogenes is a known cause of meningitis, but cerebral abscess formation is exceedingly rare. we describe a patient who presented with an unsteady gait and a small ring-enhancing lesion in the right parietal lobe. pathologic evaluation demonstrated an abscess with associated microglial nodules, and culture revealed listeria. in spite of the rarity of this entity, immunosuppression has been noted to be a predisposing factor. our patient had diabetes mellitus and chronic obstructive p ... | 2013 | 22872959 |
| a putative merr family regulator involved in biofilm formation in listeria monocytogenes 4b g. | the capability of forming biofilm makes the foodborne pathogen listeria monocytogenes more resistant to environmental stresses. to examine the mechanism of biofilm formation, l. monocytogenes transposon mutant strain gb8 with decreased ability to form biofilm was characterized in this study. southern blot assay revealed a single copy of transposon in the gb8 chromosome, and the insertion site was identified by inverse polymerase chain reaction (ipcr). the expression of lm.g_1497 was found to be ... | 2012 | 22870986 |
| pulsed-field gel electrophoresis, conventional, and molecular serotyping of listeria monocytogenes from food proficiency testing trials toward an harmonization of subtyping at european level. | the european union reference laboratory for listeria monocytogenes (eurl for l. monocytogenes) coordinates a european network of 29 national reference laboratories (nrls). depending on a national decision, nrls undertake food, environmental, and veterinary l. monocytogenes strain surveillance in their respective countries. in the framework of the pulsenet europe network, two pulsed-field gel electrophoresis (pfge) subtyping proficiency testing (pt) trials were carried out in 2003 and 2006. the o ... | 2012 | 22870985 |
| unusual pseudotumoral right atrial involvement in listeria monocytogenes septicemia. | | 2012 | 22869861 |
| hospital-acquired listeriosis associated with sandwiches in the uk: a cause for concern. | hospital-acquired outbreaks of listeriosis are not commonly reported but remain a significant public health problem. | 2012 | 22868087 |
| survival of listeria monocytogenes, escherichia coli o157:h7, and salmonella spp. on catfish fillets exposed to microwave heating in a continuous mode. | microwave (mw) heating using continuous power output with feedback control and a modified ingredient formulation may provide better and consistent cooking of foods. currently, household units with build-in inverter power supply units are available. these new generation mw ovens provide continuous, adjustable output and cooking, in contrast to the traditional rectifier-based ovens that rely on the on-off mechanism for control. this study attempted to apply a feedback power control (termed as modi ... | 2012 | 22860585 |
| genomic characterization of listeria monocytogenes strains involved in a multistate listeriosis outbreak associated with cantaloupe in us. | a multistate listeriosis outbreak associated with cantaloupe consumption was reported in the united states in september, 2011. the outbreak investigation recorded a total of 146 invasive illnesses, 30 deaths and one miscarriage. subtyping of the outbreak associated clinical, food and environmental isolates revealed two serotypes (1/2a and 1/2b) and four pulsed-field gel electrophoresis two-enzyme pattern combinations i, ii, iii, and iv, including one rarely seen before this outbreak. a dna-micro ... | 2012 | 22860127 |
| resveratrol inhibits inflammation induced by heat-killed listeria monocytogenes. | resveratrol is a polyphenolic compound in red wine that has antioxidant and cardioprotective effects in animal models. listeria monocytogenes is a pathogen that mainly affects immunocompromised individuals and is initially detected at the cell surface or in phagosomes by toll-like receptor 2. many antioxidants also exert anti-inflammatory activities; therefore, we evaluated the anti-inflammatory properties of resveratrol by studying the various inflammatory responses induced by heat-killed l. mo ... | 2012 | 22857612 |
| performance of three culture media commonly used for detecting listeria monocytogenes. | listeria monocytogenes poses a serious threat to public health, and the majority of cases of human listeriosis are associated with contaminated food. reliable microbiological testing is needed for effective control of this pathogen by the food industry and competent authorities. the aim of this study was to determine the performance of three culture media commonly used for detecting l. monocytogenes in foods. minced pork meat samples (n = 100) were subjected to microbiological testing for l. mon ... | 2012 | 22856580 |
| evaluation of methods to assess the biofilm-forming ability of listeria monocytogenes. | the contamination of ready-to-eat products with listeria monocytogenes has been related to the presence of biofilms in production lines, as biofilms protect cells from chemical sanitizers. the ability of l. monocytogenes to produce biofilms is often evaluated using in vitro methodologies. this work aims to compare the most frequently used methodologies, including high-throughput screening methods based on microplates (crystal violet and the calgary biofilm device) and methods based on cfu enumer ... | 2012 | 22856564 |
| effects of lactic acid on the growth characteristics of listeria monocytogenes on cooked ham surfaces. | the surfaces of ready-to-eat meats are susceptible to postprocessing contamination by listeria monocytogenes. this study examined and modeled the growth characteristics of l. monocytogenes on cooked ham treated with lactic acid solutions (la). cooked ham was inoculated with l. monocytogenes (ca. 10(3) cfu/g), immersed in 0, 0.5, 0.75, 1.0, 1.25, 1.5, and 2.0% la for 30 min, vacuum packaged, and stored at 4, 8, 12, and 16°c. la immersion resulted in <0.7 log cfu/g immediate reduction of l. monocy ... | 2012 | 22856563 |
| survival of salmonella, escherichia coli o157:h7, and listeria monocytogenes on inoculated almonds and pistachios stored at -19, 4, and 24° c. | the survival of salmonella, escherichia coli o157:h7, and listeria monocytogenes was determined on almonds and pistachios held at typical storage temperatures. almond kernels and inshell pistachios were inoculated with four- to six-strain cocktails of nalidixic acid-resistant salmonella, e. coli o157:h7, or l. monocytogenes at 6 log cfu/g and then dried for 72 h. after drying, inoculated nuts were stored at -19, 4, or 24°c for up to 12 months. during the initial drying period after inoculation, ... | 2012 | 22856562 |
| inactivation of escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in apple juice with gaseous ozone. | this research was initiated to assess the efficacy of gaseous ozone for inactivation escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in apple juice. juice samples with solids content of 18, 36, and 72 °brix inoculated with a culture cocktail of three foodborne pathogens were treated with gaseous ozone at a flow rate of 3.0 l/min and an ozone generation rate of 0.10, 0.90, 3.51, and 5.57 g/h for 0.5, 1, 5, and 10 min, respectively. the inactivation kinetics of gaseous ... | 2012 | 22850392 |
| phenotypic and transcriptomic analyses of sigma l-dependent characteristics in listeria monocytogenes egd-e. | in this study the phenotypic and transcriptomic traits associated with the alternative sigma factor protein sigma l in listeria monocytogenes egd-e were investigated. it was demonstrated that sigma l is required for efficient growth in presence of stress associated with food preservative measures such as low temperature and organic acids. furthermore, besides attenuation of swarming motility, the disruption of sigma l in this bacterium also reduces resistance to a diverse range of toxic compound ... | 2012 | 22850387 |
| diversity of bacterial population of table olives assessed by pcr-dgge analysis. | nocellara etnea and geracese table olives are produced according to traditional process, in which lactic acid bacteria (lab) and yeasts are the dominant microorganisms. with the aim to evaluate the effect of selected starter cultures on dynamics of bacterial population during fermentation and on growth/survival of listeria spp. artificially inoculated into the olive brine, a polyphasic approach based on the combination of culturing and pcr-dgge analysis was applied. plating results showed a diff ... | 2012 | 22850378 |
| heavy metal and disinfectant resistance of listeria monocytogenes from foods and food processing plants. | the persistence of listeria monocytogenes in food processing plants and other ecosystems reflects its ability to adapt to numerous stresses. in this study, we investigated 138 isolates from foods and food processing plants for resistance to the quaternary ammonium disinfectant benzalkonium chloride (bc) and to heavy metals (cadmium and arsenic). we also determined the prevalence of distinct cadmium resistance determinants (cada1, cada2, and cada3) among cadmium-resistant isolates. most bc-resist ... | 2012 | 22843526 |
| amplified electrochemiluminescence detection of nucleic acids by hairpin probe-based isothermal amplification. | here, we present a straightforward method for isothermal amplified detection of nucleic acids. in this proof-of-concept study, a specific dna sequence is amplified through hairpin probe-based isothermal strand-displacement polymerization reaction and then detected via a sensitive and commercially available ecl detection platform. results show that the dna sequence derived from the listeria monocytogenes hly gene can be detected down to 10 pm in solution, together with correlation of the detected ... | 2012 | 22842480 |
| a new and efficient method to obtain benzalkonium chloride adapted cells of listeria monocytogenes. | a new method to obtain benzalkonium chloride (bac) adapted l. monocytogenes cells was developed. a factorial design was used to assess the effects of the inoculum size and bac concentration on the adaptation (measured in terms of lethal dose 50 -ld50-) of 6 strains of listeria monocytogenes after only one exposure. the proposed method could be applied successfully in the l. monocytogenes strains with higher adaptive capacity to bac. in those cases, a significant empirical equation was obtained s ... | 2012 | 22841739 |
| contribution of sortase a to the regulation of listeria monocytogenes lpxtg surface proteins. | gram-positive bacteria of the genus listeria contain many surface proteins covalently bound to the peptidoglycan. in the pathogenic species listeria monocytogenes, some of these surface proteins mediate adhesion and entry into host cells. specialized enzymes called sortases anchor these proteins to the cell wall by a mechanism involving processing and covalent linkage to the peptidoglycan. how bacteria coordinate the production of sortases and their respective protein substrates is currently unk ... | 2012 | 22837151 |
| attenuated listeria monocytogenes, a mycobacterium tuberculosis esat-6 antigen expression and delivery vector for inducing an immune response. | we selectively expressed protective mycobacterium tuberculosis antigen esat-6 in recombinant strains lm(esat-6) and lmδacta/plcb(esat-6) to evaluate the capacity of listeria monocytogenes to deliver antigens from m. tuberculosis, and we studied the pathogenicity and immunogenicity of these strains compared with virulent parental strain yzulm4 and attenuated strain lmδacta/plcb. the two recombinant strains retained listeriolysin o hemolytic activity, escaped into the cytosol niche and established ... | 2012 | 22835946 |
| on the different growth conditions affecting silver antimicrobial efficacy on listeria monocytogenes and salmonella enterica. | silver is known to inhibit microorganisms and therefore it is an ideal candidate for its incorporation in a wide variety of materials for food applications. however, there is still a need for understanding how silver prolonged exposure to bacterial contamination affects the bioavailability of the active silver species. in the present study, growth curves of listeria monocytogenes and salmonella enterica were performed for 3-5 days in tryptic soy broth (tsb) and m9 minimal medium (m9) in the pres ... | 2012 | 22835228 |
| [effect of volatile metabolites from germinating seeds on the reproduction of the bacteria listeria monocytogenes and yersinia pseudotuberculosis]. | the biological activity of volatile metabolites of germinating seeds of cabbage (brassica oleacia), carrot (daukus carota), salad (lactuca sativa), and corn (zea mays l.) against listeria monocytogenes and yersinia pseudotuberculosis was studied. it was shown that volatile metabolites are transfer factors and can be the sole carbon and energy source for these bacteria. methanol is the main substance affecting their growth and reproduction. | 2015 | 22834302 |
| reduction in resident microflora, and experimentally inoculated salmonella enterica, on spinach leaves treated with vinegar and canola oil. | in this study, we explored the use of vinegar, or vinegar and canola oil as a salad dressing, to reduce bacterial levels on spinach leaves. we found that incubation of spinach leaves with various types of vinegar substantially reduced the predominantly gram-negative microflora. a similar response was observed when spinach leaves were incubated with white vinegar mixed in various proportions with canola oil, as used in salad dressing. we assessed the effects of vinegar, or vinegar and oil, on spi ... | 2012 | 22828283 |
| characterization and antimicrobial resistance of listeria monocytogenes isolated from retail beef meat in poland. | four hundred seventeen retail beef meat samples purchased in the eastern part of poland during october 2009 to january 2011 were tested for the presence of listeria monocytogenes. it was found that 81 (19.4%) of them were positive for this microorganism as identified by the culture and polymerase chain reaction (pcr) methods. molecular serotyping performed by pcr revealed that the majority of the isolates (50 strains; 61.7%) were of 1/2a serotype. furthermore, 26 (32.1%) l. monocytogenes strains ... | 2012 | 22827491 |
| roles of four putative dead-box rna helicase genes in growth of listeria monocytogenes egd-e under heat, ph, osmotic, ethanol, and oxidative stress conditions. | to examine the role of the four putative dead-box rna helicase genes of listeria monocytogenes egd-e in stress tolerance, the growth of the δlmo0866, δlmo1246, δlmo1450, and δlmo1722 deletion mutant strains at 42.5°c, at ph 5.6 or ph 9.4, in 6% nacl, in 3.5% ethanol, and in 5 mm h(2)o(2) was studied. restricted growth of the δlmo0866 deletion mutant strain in 3.5% ethanol suggests that lmo0866 contributes to ethanol stress tolerance of l. monocytogenes egd-e. the δlmo1450 mutant strain showed ne ... | 2012 | 22820328 |
| longitudinal study on the sources of listeria monocytogenes contamination in cold-smoked salmon and its processing environment in italy. | the aim of the present study was to investigate the sources of listeria monocytogenes contamination in a cold smoked salmon processing environment over a period of six years (2003-2008). a total of 170 samples of raw material, semi-processed, final product and processing surfaces at different production stages were tested for the presence of l. monocytogenes. the l. monocytogenes isolates were characterized by multiplex pcr for the analysis of virulence factors and for serogrouping. the routes o ... | 2012 | 22819714 |
| the 3bp2 adapter protein is required for chemoattractant-mediated neutrophil activation. | 3bp2 is a pleckstrin homology and src homology 2 domain-containing adapter protein mutated in cherubism, a rare autosomal-dominant human bone disorder. previously, we have demonstrated a functional role for 3bp2 in peripheral b cell development and in peritoneal b1 and splenic marginal zone b cell-mediated ab responses. in this study, we show that 3bp2 is required for g protein-coupled receptor-mediated neutrophil functions. neutrophils derived from 3bp2-deficient (sh3bp2-/-) mice failed to pola ... | 2012 | 22815290 |
| an unusual case of fatal pericarditis due to listeria monocytogenes. | pericarditis due to listeria monocytogenes is a very uncommon and serious disease. we describe a case of fatal subacute pericarditis that was caused by l. monocytogenes in a 61-year-old woman with hodgkin's disease who was diagnosed in 1975 and considered cured. in addition, we review the literature on this condition. | 2012 | 22814153 |
| rosmarinus officinalis l. essential oil and the related compound 1,8-cineole do not induce direct or cross-protection in listeria monocytogenes atcc 7644 cultivated in meat broth. | listeria monocytogenes has the capability of adapting to 1 or more antimicrobial compounds or procedures applied by the food industry to control the growth and survival of microorganisms in foods. in this study, the effects of rosmarinus officinalis essential oil (eo) and the related compound 1,8-cineole on the inhibition of the growth and survival of l. monocytogenes atcc 7644 were determined. the ability of the r. officinalis eo and 1,8-cineole to induce direct and cross-protection of bacteria ... | 2012 | 22808924 |
| remodeling of the listeria monocytogenes cell wall inside eukaryotic cells. | listeria monocytogenes is an intracellular gram-positive bacterial pathogen that produces many types of surface proteins. to get insights into its intracellular lifestyle, we used high-resolution mass spectrometry to characterize the cell wall proteome of bacteria proliferating within the eukaryotic cell. the relative amount of a few surface proteins was found notoriously different in intracellular bacteria. internalin a (inla), which is covalently bound to the peptidoglycan and plays a central ... | 2012 | 22808321 |
| characterization of medium chain length (r)-3-hydroxycarboxylic acids produced by streptomyces sp. jm3 and the evaluation of their antimicrobial properties. | (r)-3-hydroxycarboxylic acids, chiral enantiomers of bacterial polyhydroxyalkanoates (pha), may be valuable synthons for the production of numerous industrial materials such as β-lactams, fungicides, flavors, pheromones and vitamins. in this study, (r)-3-hydroxycarboxylic acid [(r)-3has)] synthons were produced by streptomyces sp. jm3 (jn166713) under batch fermentation. initial confirmation of pha production was achieved by matrix assisted laser desorption ionization-time of flight mass spectro ... | 2012 | 22806719 |
| ruminant organotypic brain-slice cultures as a model for the investigation of cns listeriosis. | central nervous system (cns) infections in ruminant livestock, such as listeriosis, are of major concern for veterinary and public health. to date, no host-specific in vitro models for ruminant cns infections are available. here, we established and evaluated the suitability of organotypic brain-slices of ruminant origin as in vitro model to study mechanisms of listeria monocytogenes cns infection. ruminants are frequently affected by fatal listeric rhombencephalitis that closely resembles the sa ... | 2012 | 22804762 |
| development and evaluation of a new device to effectively detach micro-organisms from food samples. | to develop a new instrument of great versatility for recovering micro-organisms from all types of food samples and to compare the effects with existing sample preparation methods. | 2012 | 22803600 |
| attachment of salmonella serovars and listeria monocytogenes to stainless steel and plastic conveyor belts. | in poultry industry, cross-contamination due to processing equipment and contact surfaces is very common. this study examined the extent of bacterial attachment to 6 different types and design of conveyor belts: stainless steel-single loop, stainless steel-balance weave, polyurethane with mono-polyester fabric, acetal, polypropylene mesh top, and polypropylene. clean conveyor belts were immersed separately in either a cocktail of salmonella serovars (salmonella typhimurium and salmonella enterit ... | 2012 | 22802197 |
| glycolipid antigens for treating hepatic colorectal cancer metastases and their effect on the therapeutic efficacy of live attenuated listeria monocytogenes. | previous work demonstrated that a subset of natural killer t cells in mice decreased the antitumor efficacy of live attenuated listeria monocytogenes where the actin a and internalin b genes were genetically deleted (lmd) against murine hepatic colorectal cancer metastases. therefore, we hypothesized that the use of specific glycolipids known to selectively stimulate natural killer t-cell subsets used alone or co-administered with lmd would increase survival. we found that early or multiple admi ... | 2012 | 22785644 |
| evaluation of near-infrared pasteurization in controlling escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in ready-to-eat sliced ham. | this study was conducted to investigate the efficacy of near-infrared (nir) heating to reduce salmonella enterica serovar typhimurium, escherichia coli o157:h7, and listeria monocytogenes in ready-to-eat (rte) sliced ham compared to conventional convective heating, and the effect of nir heating on quality was determined by measuring the color and texture change. a cocktail of three pathogens was inoculated on the exposed or protected surfaces of ham slices, followed by nir or conventional heatin ... | 2012 | 22773635 |
| a case of bovine raw milk contamination with listeria monocytogenes. | during routine sampling of bulk raw milk on a dairy farm, the pathogenic bacteria listeria monocytogenes was found to be a contaminant, at numbers < 100 cfu/ml. a strain with an indistinguishable pulsed-field gel electrophoresis pattern was isolated from the bulk milk two months later. environmental swabs taken at the dairy environment were negative for the presence of l. monocytogenes, indicating a possible case of excretion of the l. monocytogenes directly into the milk. milk samples were coll ... | 2012 | 22769601 |
| an atomic-force basis for the bacteriolytic effects of granulysin. | while granulysin has been suggested to play an important role in adaptive immune responses against bacterial infections by killing pathogens, and molecular force for protein-protein interaction or protein-bacteria interaction may designate the specific functions of a protein, the molecular-force basis underlying the bacteriolytic effects of granulysin at single-molecule level remains unknown. here, we produced and purified bactericidal domain of macaque granulysin (gnl). our bacterial lysis assa ... | 2012 | 22766293 |
| fluctuation in contamination dynamics of l. monocytogenes in quargel (acid curd cheese) lots recalled during the multinational listeriosis outbreak 2009/2010. | for the first time it has been possible to determine the contamination level of listeria monocytogenes in the very cheese lots of acid curd cheese that caused a multinational outbreak between 2009/2010. the listeriosis outbreak accounted for 34 clinical cases and eight deaths. the cheese, which was distributed in austria, germany, the czech republic, poland and slovakia, was recalled on the 23rd january 2010. all recalled lots were immediately investigated after call back from the retail market. ... | 2012 | 22762998 |
| resveratrol and some glucosyl, glucosylacyl, and glucuronide derivatives reduce escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes scott a adhesion to colonic epithelial cell lines. | the efficacy of resveratrol and some glucosyl, glucosylacyl, and glucuronide derivatives in inhibiting the adhesion of salmonella typhimurium, escherichia coli o157:h7, and listeria monocytogenes scott a to caco-2 and ht-29 colonic cells was investigated. the three bacteria strains were capable of adhering to both colonic epithelial cell lines, which responded by producing the pro-inflammatory interleukin 8 (il-8). adhesion inhibition of e. coli o157:h7 and s. typhimurium to colonic cells was ≥6 ... | 2012 | 22762450 |
| listeria monocytogenes in ready-to-eat seafood and potential hazards for the consumers. | the risk of exposure to listeria monocytogenes (l. monocytogenes) when consuming ready-to-eat (rte) seafood was assessed in the veneto region (italy). thirty-eight samples were analyzed, each sample consisted of three subunits belonging to the same batches. the first of the three units was examined immediately, the second was stored at +4°c (for all of its shelf-life) and the third at +10°c (for the latter third of its shelf-life) before the analysis. chemical-physical and microbiological parame ... | 2012 | 22761621 |
| listeria monocytogenes meningitis in an immunocompetent adult: a case report. | listeria monocytogenes (l. monocytogenes) is an uncommon cause of bacterial meningitis in immunocompetent adults. patients with immunosuppression are at increased risk of developing serious invasive diseases, particularly meningitis. we describe a case of meningitis caused by l. monocytogenes in an immunocompetent and previously healthy 34-year-old adult. the patient received treatment with intravenous ampicillin plus amikacin and made a full recovery. l. monocytogenes should be suspected in imm ... | 2012 | 22760149 |
| chronic activation of the kinase ikkβ impairs t cell function and survival. | activation of the transcription factor nf-κb is critical for cytokine production and t cell survival after tcr engagement. the effects of persistent nf-κb activity on t cell function and survival are poorly understood. in this study, using a murine model that expresses a constitutively active form of inhibitor of nf-κb kinase β (caikkβ) in a t cell-specific manner, we demonstrate that chronic inhibitor of nf-κb kinase β signaling promotes t cell apoptosis, attenuates responsiveness to tcr-mediat ... | 2012 | 22753932 |
| listeria monocytogenes serotypes in human infections (italy, 2000-2010). | in developed countries invasive listeriosis is an infection of great concern to public health to due its clinical severity and high fatality rate, despite its low incidence. in europe, statistically significant increasing trends in listeriosis notification rates from 2005 to 2009 were noted in austria, denmark, hungary, italy, spain and sweden. | 2012 | 22751557 |
| pfge characterisation and adhesion ability of listeria monocytogenes isolates obtained from bovine carcasses and beef processing facilities. | listeria monocytogenes is a pathogen capable of adhering to many surfaces and forming biofilms, which may explain its persistence in food processing environments. this study aimed to genetically characterise l. monocytogenes isolates obtained from bovine carcasses and beef processing facilities and to evaluate their adhesion abilities. dna from 29 l. monocytogenes isolates was subjected to enzymatic restriction digestion (asci and apai), and two clusters were identified for serotypes 4b and 1/2a ... | 2012 | 22748307 |
| inactivation kinetics of listeria monocytogenes by high-pressure processing: pressure and temperature variation. | the enhanced quasi-chemical kinetics (eqck) model is presented as a methodology to evaluate the nonlinear inactivation kinetics of baro-resistant listeria monocytogenes in a surrogate protein food system by high-pressure processing (hpp) for various combinations of pressure (p= 207 to 414 mpa) and temperature (t= 20 to 50 °c). the eqck model is based on ordinary differential equations derived from 6 "quasi-chemical reaction" steps. the eqck model continuously fits the conventional stages of the ... | 2012 | 22748039 |
| physical and antimicrobial properties of peppermint oil nanoemulsions. | the mixture of peppermint oil (po) with medium-chain triacylglycerol was emulsified in water and stabilized with a food-grade biopolymer, modified starch, to form po nanoemulsions. the effects of emulsifying conditions including homogenization pressure, the number of processing cycles, and oil loading on the mean diameters and viscosities of nanoemulsions were characterized by dynamic light scattering, optical microscopy, and rheological measurements. the formulated po nanoemulsions with mean di ... | 2012 | 22746096 |
| bacteriophage amplification assay for detection of listeria spp. using virucidal laser treatment. | a protocol for the bacteriophage amplification technique was developed for quantitative detection of viable listeria monocytogenes cells using the a511 listeriophage with plaque formation as the end-point assay. laser and toluidine blue o (tbo) were employed as selective virucidal treatment for destruction of exogenous bacteriophage. laser and tbo can bring a total reduction in titer phage (ca. 10(8) pfu/ml) without affecting the viability of l. monocytogenes cells. artificially inoculated skimm ... | 2012 | 24031937 |
| ether lipid vesicle-based antigens impart protection against experimental listeriosis. | incidence of food-borne infections from listeria monocytogenes, a parasite that has adapted intracellular residence to avoid antibody onslaught, has increased dramatically in the past few years. the apparent lack of an effective vaccine that is capable of evoking the desired cytotoxic t cell response to obliterate this intracellular pathogen has encouraged the investigation of alternate prophylactic strategies. it should also be noted that archaebacteria (archae) lipid-based adjuvants enhance th ... | 2012 | 22745536 |
| multieffector-functional immune responses of hmbpp-specific vγ2vδ2 t cells in nonhuman primates inoculated with listeria monocytogenes δacta prfa*. | although listeria monocytogenes can induce systemic infection causing spontaneous abortion, septicemia, and meningitis, studies have not been performed to investigate human anti-l. monocytogenes immune responses, including those of ag-specific vγ2vδ2 t cells, a dominant human γδ t cell subset. l. monocytogenes is the only pathogen known to possess both the mevalonate and non-mevalonate isoprenoid biosynthesis pathways that produce metabolic phosphates or phosphoantigens activating human vγ2vδ2 t ... | 2012 | 22745375 |
| the listeria monocytogenes serotype 4b autolysin ispc has n-acetylglucosaminidase activity. | ispc is a novel peptidoglycan (pg) hydrolase that is conserved in listeria monocytogenes serotype 4b strains and is involved in virulence. the aim of this study was to establish the hydrolytic bond specificity of ispc. purified l. monocytogenes peptidoglycan was digested by recombinant ispc and the resulting muropeptides were separated by reverse phase high-performance liquid chromatography. the structure of each muropeptide was determined using matrix-assisted laser desorption ionization (maldi ... | 2012 | 22730466 |
| genetic diversity and molecular typing of listeria monocytogenes in china. | listeria monocytogenes can cause invasive diseases in humans and farm animals and is frequently isolated from dairy products and poultry. listeriosis is uncommon in china but l. monocytogenes has been isolated from foods and food processing environments in china. however little is known of genetic diversity of chinese l. monocytogenes isolates and their relationships with global isolates. | 2012 | 22727037 |
| conditional stat1 ablation reveals the importance of interferon signaling for immunity to listeria monocytogenes infection. | signal transducer and activator of transcription 1 (stat1) is a key player in responses to interferons (ifn). mutations of stat1 cause severe immune deficiencies in humans and mice. here we investigate the importance of stat1 signaling for the innate and secondary immune response to the intracellular bacterial pathogen listeria monocytogenes (lm). cell type-restricted ablation of the stat1 gene in naïve animals revealed unique roles in three cell types: macrophage stat1 signaling protected again ... | 2012 | 22719255 |
| innate ifn-γ is essential for programmed death ligand-1-mediated t cell stimulation following listeria monocytogenes infection. | although best characterized for sustaining t cell exhaustion during persistent viral infection, programmed death ligand-1 (pdl-1) also stimulates the expansion of protective t cells after infection with intracellular bacterial pathogens. therefore, establishing the molecular signals that control whether pdl-1 stimulates immune suppression or activation is important as immune modulation therapies based on manipulating pdl-1 are being developed. in this study, the requirement for pdl-1 blockade in ... | 2012 | 22711893 |
| a listeria monocytogenes rna helicase essential for growth and ribosomal maturation at low temperatures uses its c terminus for appropriate interaction with the ribosome. | listeria monocytogenes, a gram-positive food-borne human pathogen, is able to grow at temperatures close to 0°c and is thus of great concern for the food industry. in this work, we investigated the physiological role of one dexd-box rna helicase in listeria monocytogenes. the rna helicase lmo1722 was required for optimal growth at low temperatures, whereas it was dispensable at 37°c. a δlmo1722 strain was less motile due to downregulation of the major subunit of the flagellum, flaa, caused by de ... | 2012 | 22707705 |
| [listeria monocytogenes endocarditis: two case reports with poor outcome]. | | 2012 | 22707109 |
| listeria septicaemia following insertion of a dynamic hip screw: a case report and literature review. | listeria monocytogenes is a food borne bacterial pathogen which is rarely encountered in the united kingdom. this rare and potentially life threatening infection has a high mortality rate and therefore requires early antimicrobial intervention. | 2012 | 22706298 |
| antimicrobial activity of lactic acid bacteria against listeria monocytogenes on frankfurters formulated with and without lactate/diacetate. | contamination by listeria monocytogenes has been a constant public health threat for the ready-to-eat (rte) meat industry due to the potential for high mortalities from listeriosis. lactic acid bacteria (lab) have shown protective action against various pathogenic bacteria. the aim of this study was to evaluate the antilisterial activity of a combination of three lab strains (lactiguard®) on l. monocytogenes. the combination of the lab was inhibitory to l. monocytogenes inoculated onto frankfurt ... | 2012 | 22704134 |
| estimation of listeria monocytogenes transfer coefficients and efficacy of bacterial removal through cleaning and sanitation. | listeria monocytogenes is readily found in the environment of retail deli establishments and can occasionally contaminate food handled in these establishments. here we synthesize the available scientific evidence to derive probability distributions and mathematical models of bacterial transfers between environmental surfaces and foods, including those during slicing of food, and of bacterial removal during cleaning and sanitizing (models available at www.foodrisk.org). transfer coefficients vari ... | 2012 | 22704063 |
| mechanisms of iron and haem transport by listeria monocytogenes. | listeria monocytogenes, the causative agent of listeriosis, is a virulent foodborne gram-positive bacterial pathogen, with 20-30% mortality. it has a broad ability to transport iron, either in the form of ferric siderophores, or by extracting it from mammalian iron binding proteins. in this review we focus on the mechanisms of ferric siderophore and haem transport into the listerial cell. despite the fact that it does not synthesize siderophores, l. monocytogenes transports ferric siderophores i ... | 2013 | 22703022 |
| intracellular vesicles as reproduction elements in cell wall-deficient l-form bacteria. | cell wall-deficient bacteria, or l-forms, represent an extreme example of bacterial plasticity. stable l-forms can multiply and propagate indefinitely in the absence of a cell wall. data presented here are consistent with the model that intracellular vesicles in listeria monocytogenes l-form cells represent the actual viable reproductive elements. first, small intracellular vesicles are formed along the mother cell cytoplasmic membrane, originating from local phospholipid accumulation. during gr ... | 2012 | 22701656 |
| machine learning analysis of the relationship between changes in immunological parameters and changes in resistance to listeria monocytogenes: a new approach for risk assessment and systems immunology. | no method has been reported to predict, even approximately, the impact of mild-to-moderate changes in several immunological parameters on resistance to infection. the ability to make such predictions would be useful in risk assessment. in addition, equations that predict host resistance on the basis of changes in components of a complex biological system (the immune system) would fulfill one of the major goals of systems biology. in this study, multiple machine learning classification methods we ... | 2012 | 22696237 |
| comparison of sampling procedures for recovery of listeria monocytogenes from stainless steel food contact surfaces. | a number of techniques exist for microbiological sampling of food processing environments in food industries. in the present study the efficacies of nine sampling procedures for the recovery of listeria monocytogenes from food contact surfaces, including a new sampling device consisting of a miniroller, were evaluated and compared. a stainless steel table was inoculated with l. monocytogenes strain 935 (serovar 4b, human origin) and l. monocytogenes strain 437/07 (serovar 1/2b, food origin), at ... | 2012 | 22691475 |
| inhibition of listeria monocytogenes using natural antimicrobials in no-nitrate-or-nitrite-added ham. | consumer demand for foods manufactured without the direct addition of chemical preservatives, such as sodium nitrite and organic acid salts, has resulted in a unique class of "naturally" cured meat products. formulation with a natural nitrate source and nitrate-reducing bacteria results in naturally cured processed meats that possess traits similar to conventionally cured meats. however, previous research has shown that the naturally cured products are more susceptible to pathogen growth. this s ... | 2012 | 22691474 |
| control of listeria monocytogenes on frankfurters and cooked pork chops by irradiation combined with modified atmosphere packaging. | this study was conducted to investigate the efficacy of controlling listeria monocytogenes on frankfurters and cooked pork chops with irradiation and modified atmosphere packaging (map) containing a high concentration of co(2). frankfurters and cooked pork chops were inoculated with a five-strain cocktail of l. monocytogenes and packaged in vacuum or high-co(2) map. irradiation was applied to each product at 0, 0.5, 1.0, or 1.5 kgy. no significant packaging effect was found for the radiation sen ... | 2012 | 22691473 |
| viability of salmonella and listeria monocytogenes in delicatessen salads and hummus as affected by sodium content and storage temperature. | a study was conducted to determine survival and growth behavior of salmonella and listeria monocytogenes in commercially prepared mayonnaise-based potato salad, macaroni salad, and coleslaw and in hummus (initial mean ph values were 4.80 to 4.94, 4.18 to 4.31, 3.87, and 4.50 to 4.52, respectively) as affected by sodium concentration (133 to 364, 190 to 336, 146 to 272, and 264 to 728 mg/100 g, respectively) and storage at 4 or 10°c for up to 27 days. salmonella did not grow in any of the test pr ... | 2012 | 22691471 |
| application of non-linear models to predict inhibition effects of various plant hydrosols on listeria monocytogenes inoculated on fresh-cut apples. | in this study, we studied the effects of some plant hydrosols obtained from bay leaf, black cumin, rosemary, sage, and thyme in reducing listeria monocytogenes on the surface of fresh-cut apple cubes. adaptive neuro-fuzzy inference system (anfis), artificial neural network (ann), and multiple linear regression (mlr) models were used for describing the behavior of l. monocytogenes against the hydrosol treatments. approximately 1-1.5 log cfu/g decreases in l. monocytogenes counts were observed aft ... | 2012 | 22690764 |
| genome sequence of listeria monocytogenes 07pf0776, a cardiotropic serovar 4b strain. | listeria monocytogenes is a food-borne bacterial pathogen commonly associated with serious invasive infections of the central nervous system or of the developing fetus. we present the genome sequence of listeria monocytogenes 07pf0776, a serovar 4b isolate from a human myocardial abscess that exhibits enhanced invasion of cardiac tissue. | 2012 | 22689239 |
| granulomatous typhlocolitis, lymphangitis, and lymphadenitis in a horse infected with listeria monocytogenes, salmonella typhimurium, and cyathostomes. | a 15-year-old american quarter horse mare was euthanized because of poor response to therapy for severe diarrhea. significant gross findings were limited to the large intestines. the walls of the cecum and colon were thickened with widely scattered nodules in the mucosa and submucosa that extended into the enlarged colic lymph nodes. microscopically, there was severe granulomatous typhlocolitis, lymphangitis, and lymphadenitis, with many intralesional gram-positive, non-acid-fast coccobacilli an ... | 2013 | 22688587 |
| a piezoelectric immunosensor for specific capture and enrichment of viable pathogens by quartz crystal microbalance sensor, followed by detection with antibody-functionalized gold nanoparticles. | a sensitive bacteria enrichment and detection system for viable escherichia coli o157:h7 was developed using a piezoelectric biosensor-quartz crystal microbalance (qcm) with antibody-functionalized gold nanoparticles (aunps) used as detection verifiers and amplifiers. in the circulating-flow qcm system, capture antibodies for e. coli o157:h7 were first immobilized onto the qcm chip. the sample containing e. coli o157:h7 was circulated through the system in the presence of 10 ml of brain heart in ... | 2016 | 22683250 |
| pregnancy-specific glycoprotein 1a activates dendritic cells to provide signals for th17-, th2-, and treg-cell polarization. | because of their plasticity and central role in orchestrating immunity and tolerance, dcs can respond to pregnancy-specific signals, thus promoting the appropriate immune response in order to support pregnancy. here, we show that pregnancy-specific glycoprotein (psg1a), the major variant of psg released into the circulation during pregnancy, targets dcs to differentiate into a subset with a unique phenotype and function. this semi-mature phenotype is able to secrete il-6 and tgf-β. psg1a also af ... | 2012 | 22678910 |
| highly discriminatory typing method for listeria monocytogenes using polymorphic tandem repeat regions. | tandem repeats (tr), which are repetitive nucleotide sequences in dna, are polymorphic both in repeat number and sequence. in this study, we developed a new typing method, multilocus tr sequence analysis (mltsa), for the foodborne pathogen listeria monocytogenes using sequence polymorphisms in three tandem repeat regions. the obtained dendrogram clustered l. monocytogenes strains of lineage i and lineage ii separately, and formed three groups within the lineage i cluster, each of which included ... | 2012 | 22677602 |
| early events regulating immunity and pathogenesis during listeria monocytogenes infection. | listeria monocytogenes (lm) is both a life-threatening pathogen of humans and a model organism that is widely used to dissect the mechanisms of innate and adaptive immune resistance to infection. specific aspects of the immune response to systemic lm infection can be protective, neutral, or in some cases deleterious. in this review, we seek to provide an overview of the early events during lm infection that dictate or regulate host innate and adaptive immune responses. we highlight several recen ... | 2012 | 22677184 |
| expression of ferritin-like protein in listeria monocytogenes after cold and freezing stress. | the cold shock protein family consists of the transfer of the foodborne pathogen listeria monocytogenes from 37 to 4 and -20 °c and was characterized by the sharp induction of a low molecular mass protein. this major cold shock protein ferritin-like protein (flp) has an important role in regulation of various microbial physiological processes. flp have a molecular mass of about 18 kda, as observed on sds-page. the purification procedure including ammonium sulfate fractionation was used. monospec ... | 2012 | 22674417 |
| [presence of listeria monocytogenes in ready-to-eat foods available in open markets, delicatessens and supermarkets, bogotá, 2002-2008]. | the increase in diseases transmitted by foods has become a public health problem. listeria monocytogenes, the etiological agent of listeriosis or typical food poisoning,is considered to be among the most important food borne illnesses. high risk foods for l.monocytogenes have been recognized as foods already prepared for consumption and made available in retail outlets. | 2012 | 22674319 |
| inactivation of listeria monocytogenes on ham and bologna using pectin-based apple, carrot, and hibiscus edible films containing carvacrol and cinnamaldehyde. | edible films can be used as wrapping material on food products to reduce surface contamination. the incorporation of antimicrobials into edible films could serve as an additional barrier against pathogenic and spoilage microorganisms that contaminate food surfaces. the objective of this study was to investigate the antimicrobial effects of carvacrol and cinnamaldehyde, incorporated into apple, carrot, and hibiscus-based edible films against listeria monocytogenes on contaminated ham and bologna. ... | 2012 | 22671718 |
| prevalence and serotypes of listeria monocytogenes contamination in chinese beef processing plants. | the aim of this work was to study the epidemiology of listeria spp., particularly listeria monocytogenes, and to identify the serotypes present in contaminated samples from beef processing plants in china. a total of 439 samples were obtained from bovine feces, hides, and carcasses at three commercial processing plants. a standard protocol (iso 11290-1) was followed to detect listeria spp. and l. monocytogenes, and multiplex polymerase chain reaction was used to identify the various l. monocytog ... | 2012 | 22663187 |
| bacterial infections other than spontaneous bacterial peritonitis in cirrhosis. | cirrhotic patients are immunocompromised with a high risk of infection. proinflammatory cytokines and hemodynamic circulation derangement further facilitate the development of serious consequences of infections. other than spontaneous bacterial peritonitis, bacteremia and bacterial infections of other organ systems are frequently observed. gram-negative enteric bacteria are the most common causative organism. other bacterial infections, such as enterococci, vibrio spp., aeromonas spp., clostridi ... | 2012 | 22662285 |
| left ventricular outflow tract to left atrium fistula due to non-valve listeria monocytogenes endocarditis. | | 2012 | 22659197 |
| rugulactone and its analogues exert antibacterial effects through multiple mechanisms including inhibition of thiamine biosynthesis. | rugulactone is a dihydro-α-pyrone isolated from the plant cryptocarya rugulosa in 2009. it has been reported to display ikb kinase (ikk) inhibitory activity, as well as antibiotic activity in several strains of pathogenic bacteria. however, its biological targets and mode of action in bacteria have not yet been explored. here we present enantioselective syntheses of rugulactone and of some corresponding activity-based protein profiling (abpp) probes. we found that the abpp probes in this study a ... | 2012 | 22653914 |
| listeriolysin o: the swiss army knife of listeria. | listeriolysin o (llo) is a toxin produced by listeria monocytogenes, an opportunistic bacterial pathogen responsible for the disease listeriosis. this disease starts with the ingestion of contaminated foods and mainly affects immunocompromised individuals, newborns, and pregnant women. in the laboratory, l. monocytogenes is used as a model organism to study processes such as cell invasion, intracellular survival, and cell-to-cell spreading, as this gram-positive bacterium has evolved elaborate m ... | 2012 | 22652164 |
| synergistic effect of steam and lactic acid against escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes biofilms on polyvinyl chloride and stainless steel. | this study was designed to investigate the individual and combined effects of steam and lactic acid (la) on the inactivation of biofilms formed by escherichia coli o157:h7, salmonella typhimurium, and listeria monocytogenes on polyvinyl chloride (pvc) and stainless steel. six day old biofilms were developed on pvc and stainless steel coupons by using a mixture of three strains each of three foodborne pathogens at 25°c. after biofilm development, pvc and stainless steel coupons were treated with ... | 2012 | 22647677 |
| distinct cd4+ helper t cells involved in primary and secondary responses to infection. | helper t cells are critical for protective immunity, cd8(+) t-cell memory, and cd4(+) recall responses, but whether the same or distinct cd4(+) t cells are involved in these responses has not been established. here we describe two cd4(+) t cells, llo118 and llo56, specific for an immunodominant listeria monocytogenes epitope, with dramatically different responses to primary and secondary infection. comparing in vivo responses, llo118 t cells proliferate more strongly to primary infection, wherea ... | 2012 | 22645349 |
| combined immunotherapy with listeria monocytogenes-based psa vaccine and radiation therapy leads to a therapeutic response in a murine model of prostate cancer. | radiation therapy (rt) is an integral part of prostate cancer treatment across all stages and risk groups. immunotherapy using a live, attenuated, listeria monocytogenes-based vaccines have been shown previously to be highly efficient in stimulating anti-tumor responses to impact on the growth of established tumors in different tumor models. here, we evaluated the combination of rt and immunotherapy using listeria monocytogenes-based vaccine (adxs31-142) in a mouse model of prostate cancer. mice ... | 2012 | 22644735 |
| bilateral anterior uveitis in a patient with bacterial meningitis. | we report a case of bilateral iridocyclitis accompanied by bacterial meningitis in an immunocompetent patient. case report. a 48 year-old healthy female visited our hospital with strong headache, fever, bilateral hyperemia, and blurred vision in both eyes. a slit-lamp examination revealed moderate cells and flare in the anterior chamber of both eyes, with fine keratoprecipitates. there were no obvious inflammatory changes in the vitreous, retina, and optic disc of both eyes. elevation of periphe ... | 2012 | 22638923 |