| inla promotes dissemination of listeria monocytogenes to the mesenteric lymph nodes during food borne infection of mice. | intestinal listeria monocytogenes infection is not efficient in mice and this has been attributed to a low affinity interaction between the bacterial surface protein inla and e-cadherin on murine intestinal epithelial cells. previous studies using either transgenic mice expressing human e-cadherin or mouse-adapted l. monocytogenes expressing a modified inla protein (inla(m)) with high affinity for murine e-cadherin showed increased efficiency of intragastric infection. however, the large inocula ... | 2012 | 23166492 |
| effect of salt reduction on growth of listeria monocytogenes in meat and poultry systems. | reducing sodium in food could have an effect on food safety. the objective was to determine differences in growth of listeria monocytogenes in meat and poultry systems with salt substitutes. for phase 1, fresh ground beef, pork, and turkey with nacl, kcl, cacl(2), mgcl(2), sea salt, or replacement salt added at 2.0% were inoculated with l. monocytogenes to determine growth/survival during 5 d at 4 °c to simulate a pre-blend process. l. monocytogenes populations significantly decreased (0.41 log ... | 2012 | 23164056 |
| vaccination with immunotherapeutic listeria monocytogenes induces il-17(+) γδ t cells in a murine model for hpv associated cancer. | interleukin 17 (il-17) is produced during infection with listeria monocytogenes and is also an important regulator of tumor development with both pro- and anti-tumorigenic effects. αβ t cells and γδ t cells are among the principle producers of il-17 in response to infection and other proinflammatory conditions. listeria-based cancer immunotherapies induce ifnγ directed th1 dependent tumor regression; however, the role of il-17 in listeria based immunotherapy has not been addressed. therefore, we ... | 2012 | 23162749 |
| activated charcoal enhanced the antigen-expression and dendritic cell maturation of the vaccine using listeria-platform. | listeria monocytogenes (lm) has been used as a vaccine vector based upon its ability to induce a strong cell-mediated immune response. lm inactivated with γ-irradiation retains immunogenic properties and is an attractive platform for clinical use since it would have improved safety concerns compared to live vectors. activated charcoal has been shown to enhance expression of lm proteins such as prfa. | 2012 | 23143471 |
| spatial positioning of cell wall-anchored virulence factors in gram-positive bacteria. | many virulence factors of gram-positive bacteria are anchored to the peptidoglycan by a sorting signal. while surface display mechanisms are well characterized, less is known about the spatial and temporal organization of these proteins in the bacterial envelope. this review summarizes recent studies on the rod-shaped listeria monocytogenes, ovococcal streptococcus pyogenes and spherical staphylococcus aureus bacteria that provide insights into the compartmentalization of the surface and distrib ... | 2012 | 23141759 |
| prevalence and level of listeria monocytogenes in ready-to-eat foods in sweden 2010. | an increasing trend in human listeriosis cases over the past five years (2005-2009) in sweden encouraged the authorities to examine the prevalence and levels of listeria monocytogenes in ready-to-eat (rte) foods in 2010. the combined results of two surveys are presented: the swedish part of an eu-wide survey and a national survey. a total of 1590 samples covering three categories of rte food able to support growth of l. monocytogenes: (i) soft and semi-soft cheeses (mould- and smear-ripened); (i ... | 2012 | 23141642 |
| industrial disinfectants do not select for resistance in listeria monocytogenes following long term exposure. | listeria monocytogenes is a food-borne pathogen that can persist for years in food processing plants. it has been hypothesized that this could be due to the development of tolerance or resistance to the disinfectants used. the purpose of the present study was to determine whether biocide resistance or tolerance would evolve in l. monocytogenes under continued selection in three industrial disinfectants. l. monocytogenes egd was exposed to desinfect cl (hypochlorite) and incimaxx des (peracedic a ... | 2012 | 23141640 |
| formylpeptide receptors are critical for rapid neutrophil mobilization in host defense against listeria monocytogenes. | listeria monocytogenes (listeria) causes opportunistic infection in immunocompromised hosts with high mortality. resistance to listeria depends on immune responses and recruitment of neutrophils of the immune system into infected sites is an early and critical step. mouse neutrophils express two g protein-coupled formylpeptide receptor subtypes fpr1 and fpr2 that recognize bacterial and host-derived chemotactic molecules including listeria peptides for cell migration and activation. here we repo ... | 2012 | 23139859 |
| characterization of microflora in latin-style cheeses by next-generation sequencing technology. | cheese contamination can occur at numerous stages in the manufacturing process including the use of improperly pasteurized or raw milk. of concern is the potential contamination by listeria monocytogenes and other pathogenic bacteria that find the high moisture levels and moderate ph of popular latin-style cheeses like queso fresco a hospitable environment. in the investigation of a foodborne outbreak, samples typically undergo enrichment in broth for 24 hours followed by selective agar plating ... | 2012 | 23134566 |
| susceptibility of listeria monocytogenes biofilms and planktonic cultures to hydrogen peroxide in food processing environments. | recent studies have indicated that listeria monocytogenes formed biofilms on the surface of food processing equipment, and may survive sanitization treatments. the purpose of this study was to compare the susceptibility of l. monocytogenes grown in either a biofilm or planktonic culture when exposed to hydrogen peroxide (h(2)o(2)). twelve strains of biofilm-forming l. monocytogenes and their planktonic counterparts were treated with various concentrations of h(2)o(2) (1, 6, and 10%), and the cel ... | 2012 | 23132560 |
| risk factors for microbial contamination in fruits and vegetables at the preharvest level: a systematic review. | the objective of this study was to perform a systematic review of risk factors for contamination of fruits and vegetables with listeria monocytogenes, salmonella, and escherichia coli o157:h7 at the preharvest level. relevant studies were identified by searching six electronic databases: medline, embase, cab abstracts, agris, agricola, and fsta, using the following thesaurus terms: l. monocytogenes, salmonella, e. coli o157 and fruit, vegetable. all search terms were exploded to find all related ... | 2012 | 23127717 |
| effect of native microflora, waiting period, and storage temperature on listeria monocytogenes serovars transferred from cantaloupe rind to fresh-cut pieces during preparation. | the most recent outbreak of listeriosis linked to consumption of fresh-cut cantaloupes indicates the need to investigate the behavior of listeria monocytogenes in the presence of native microflora of cantaloupe pieces during storage. whole cantaloupes were inoculated with l. monocytogenes (10(8)-cfu/ml suspension) for 10 min and air dried in a biosafety cabinet for 1 h and then treated (unwashed, water washed, and 2.5% hydrogen peroxide washed). fresh-cut pieces (∼3 cm) prepared from these melon ... | 2012 | 23127699 |
| entry of listeria monocytogenes in mammalian epithelial cells: an updated view. | listeria monocytogenes is a bacterial pathogen that promotes its internalization into host epithelial cells. interaction between the bacterial surface molecules inla and inlb and their cellular receptors e-cadherin and met, respectively, triggers the recruitment of endocytic effectors, the subversion of the phosphoinositide metabolism, and the remodeling of the actin cytoskeleton that lead to bacterial engulfment. additional bacterial surface and secreted virulence factors also contribute to ent ... | 2012 | 23125201 |
| structural basis of met receptor dimerization by the bacterial invasion protein inlb and the hgf/sf splice variant nk1. | the structural basis of ligand-induced dimerization of the receptor tyrosine kinase met by its natural ligand hepatocyte growth factor/scatter factor (hgf/sf) is not well understood. however, interesting insight into the molecular mechanism of met dimerization has emerged from crystal structures of met in complex with a bacterial agonist, the invasion protein internalin b (inlb) from pathogenic listeria monocytogenes. met activation by inlb promotes uptake of bacteria into host cells. structural ... | 2013 | 23123275 |
| the expression of superoxide dismutase (sod) and a putative abc transporter permease is inversely correlated during biofilm formation in listeria monocytogenes 4b g. | little is known about the molecular basis of biofilm formation in listeria monocytogenes. the superoxide dismutase (sod) of the deletion mutant of lm.g_1771 gene, which encodes for a putative abc transporter permease, is highly expressed in biofilm. in this study, the sod gene deletion mutant δsod, and double deletion mutant of the sod and lm. g_1771 genes δ1771δsod were used to investigate the role of sod and its relationship to the expression of the putative abc transporter permease in biofilm ... | 2012 | 23119031 |
| riboflavin analogs as antiinfectives: occurrence, mode of action, metabolism and resistance. | antimetabolites are molecules, which are structurally similar to molecules needed to carry out primary metabolic reactions.the inhibitory activity of an antimetabolite depends on its successful competition with the natural substrate, ligand, modulator or cofactor of a given biomolecule. antimetabolites are indispensable as molecular tools in order to understand biological processes. beyond that,antimetabolites have a large variety of applications in the pharmaceutical and food industries. the id ... | 2013 | 23116394 |
| in vivo functional requirement of the mouse ifitm1 gene for germ cell development, interferon mediated immune response and somitogenesis. | the mammalian interferon induced transmembrane protein 1 (ifitm1) gene was originally identified as a member of a gene family highly inducible by type i and type ii interferons. based on expression analyses, it was suggested to be required for normal primordial germ cell migration. the knockdown of ifitm1 in mouse embryos provided evidence for a role in somitogenesis. we generated the first targeted knockin allele of the ifitm1 gene to systematically reassess all inferred functions. sperm motili ... | 2012 | 23115618 |
| meningoencephalitis due to listeria monocytogenes in a pregnant rhesus macaque (macaca mulatta). | we here report a spontaneous case of meningoencephalitis due to listeria monocytogenes in an adult primiparous rhesus macaque (macaca mulatta) during an outbreak of listeriosis in an outdoor enclosure. clinical signs included tremors, abnormal posture, and altered mental status. hematology and analyses of cerebrospinal fluid were consistent with bacterial infection. pure cultures of l. monocytogenes were recovered from the placenta-abortus, cerebrospinal fluid, and brain tissue. the macaque did ... | 2012 | 23114049 |
| regulatory t cells increase the avidity of primary cd8+ t cell responses and promote memory. | although regulatory t cells (t(regs)) are known to suppress self-reactive autoimmune responses, their role during t cell responses to nonself antigens is not well understood. we show that t(regs) play a critical role during the priming of immune responses in mice. t(reg) depletion induced the activation and expansion of a population of low-avidity cd8(+) t cells because of overproduction of ccl-3/4/5 chemokines, which stabilized the interactions between antigen-presenting dendritic cells and low ... | 2012 | 23112334 |
| stress-induced effects, which inhibit host defenses, alter leukocyte trafficking. | acute cold restraint stress (acrs) has been reported to suppress host defenses against listeria monocytogenes, and this suppression was mediated by beta1-adrenoceptors (β1-ars). although acrs appears to inhibit mainly early innate immune defenses, interference with leukocyte chemotaxis and the involvement of β1-ar (or β2-ar) signaling had not been assessed. thus, the link between sympathetic nerve stimulation, release of neurotransmitters, and changes in blood leukocyte profiles, including oxida ... | 2013 | 23111563 |
| spontaneous bacterial peritonitis caused by listeria monocytogenes: a case report and literature review. | spontaneous bacterial peritonitis (sbp) is a common and often serious complication of long standing ascites in the presence of advanced liver disease.1 we report a case of a 51- year-old man with alcoholic cirrhosis admitted to our department with jaundice, ascitis and lower limbs edema. a diagnosis of spontaneous bacterial peritonitis was made and empiric therapy with cefotaxime was prescribed with no response. three days later listeria monocytogenes was detected in peritoneal fluid culture and ... | 2013 | 23109462 |
| efficacy of household washing treatments for the control of listeria monocytogenes on salad vegetables. | the efficacy of household decontamination methods at reducing listeria monocytogenes on fresh lettuce (lactuca sativa), cucumber (cucumis sativus) and parsley (petroselinum sativum) was studied. inoculated vegetable pieces were immersed in washing solutions and surviving l. monocytogenes enumerated. parameters investigated were storage temperature prior to washing, dipping water temperature, agitation, acetic acid concentration and immersion time. the results indicated that the storage temperatu ... | 2012 | 23107504 |
| a mutant in the listeria monocytogenes fur-regulated virulence locus (frva) induces cellular immunity and confers protection against listeriosis in mice. | listeria monocytogenes is a gram-positive intracellular pathogen that is responsible for listeriosis, a potentially fatal, food-borne illness. due to its cytoplasmic location during infection, this pathogen can mediate a long-lasting cellular immune response, which makes attenuated strains strong candidates for vaccine development. recently, our group identified and characterized frva (fur-regulated virulence factor a), and deletion of this gene resulted in disruption of iron homeostasis and a s ... | 2013 | 23105022 |
| characterization of listeria monocytogenes isolates from cattle and ground beef by pulsed-field gel electrophoresis. | the aims of this study were to determine the occurrence of listeria monocytogenes in cattle feces and ground beef, to characterize these strains by pulsed-field gel electrophoresis and to compare them to three listeria strains found in humans. cattle from different origins (n = 250) and ground beef obtained from supermarkets (n = 40) were sampled. the results show low occurrence in cattle feces (0.4 %) but a higher presence in ground beef (37 %). an important part of the ground beef strains (80 ... | 2012 | 23102469 |
| dynamics of cd4(+) t cell responses against listeria monocytogenes. | the generation of cd4(+) t cell memory cells is poorly understood. recently, two different murine cd4(+) tcr transgenic t cell lines, llo118 and llo56, both specific for the same epitope but differing in their expression level of the cell surface protein cd5, were generated. notably, these cell lines showed different behavior upon primary and secondary exposure to listeria monocytogenes. whereas llo118 showed a stronger primary response and generated more robust cd8(+) t cell help upon secondary ... | 2012 | 23100516 |
| antigen processing and presentation by dendritic cells is independent of coronin 1. | coronin 1, which is a member of the evolutionary conserved coronin protein family that is highly expressed in all leukocytes is involved in the activation of the ca(2+)/calcineurin signaling pathway following cell surface stimulation in t cells, b cells as well as macrophages. mice deficient for coronin 1 have strongly reduced peripheral t cell numbers as a result of a lack of pro-survival signals for naïve t cells. whether or not impaired antigen processing and presentation in the absence of co ... | 2013 | 23099476 |
| listeriosis outbreak caused by latin-style fresh cheese, bizkaia, spain, august 2012. | two cases of laboratory-confirmed listeriosis were detected in bizkaia, spain, at the end of august. the epidemiological investigation indicated that these two cases were associated with the consumption of latin-style fresh cheese made from pasteurised milk in portugal. different batches of the same cheese were analysed and confirmed as contaminated with listeria monocytogenes. the product was withdrawn from the market and the population was advised not to consume this kind of cheese. | 2012 | 23098823 |
| development and validation of qualitative sybr®green real-time pcr for detection and discrimination of listeria spp. and listeria monocytogenes. | a combination of four qualitative sybr®green qpcr screening assays targeting two levels of discrimination: listeria genus (except listeria grayi) and listeria monocytogenes, is presented. these assays have been developed to be run simultaneously using the same polymerase chain reaction (pcr) programme. the paper also proposes a new validation procedure to specifically validate qpcr assays applied to food microbiology according to two guidelines: the iso 22118 norm and the "definition of minimum ... | 2013 | 23086339 |
| listeria monocytogenes infection in crohn's disease treated with adalimumab. | | 2012 | 23072245 |
| functional γ-aminobutyrate shunt in listeria monocytogenes: role in acid tolerance and succinate biosynthesis. | listeria monocytogenes, the causative agent of human listeriosis, is known for its ability to withstand severe environmental stresses. the glutamate decarboxylase (gad) system is one of the principal systems utilized by the bacterium to cope with acid stress, a reaction that produces γ-aminobutyrate (gaba) from glutamate. recently, we have shown that gaba can accumulate intracellularly under acidic conditions, even under conditions where no extracellular glutamate-gaba exchange is detectable. th ... | 2013 | 23064337 |
| rig-i detects infection with live listeria by sensing secreted bacterial nucleic acids. | immunity against infection with listeria monocytogenes is not achieved from innate immune stimulation by contact with killed but requires viable listeria gaining access to the cytosol of infected cells. it has remained ill-defined how such immune sensing of live listeria occurs. here, we report that efficient cytosolic immune sensing requires access of nucleic acids derived from live listeria to the cytoplasm of infected cells. we found that listeria released nucleic acids and that such secreted ... | 2012 | 23064150 |
| real-time pcrs assay for serogrouping listeria monocytogenes and differentiation from other listeria spp. | a 5'-exonuclease real-time triplex-pcr assay was developed for serogrouping listeria monocytogenes, and differentiation from other listeria spp. the assay was evaluated on 109 listeria cultures, and results were compared with a previously validated gel-based multiplex-pcr procedure. all l. monocytogenes were correctly classified into four serogroups, including atypical serotype 4b strains, and differentiated from other listeria species. the assay is a rapid method for categorisation of suspect l ... | 2013 | 23064121 |
| microparticles released by listeria monocytogenes-infected macrophages are required for dendritic cell-elicited protective immunity. | interplay between macrophages and dendritic cells in the processing and presentation of bacterial antigens for t-cell immune responses remains poorly understood. using a listeria monocytogenes (lm) infection model, we demonstrate that dendritic cells (dcs) require the support of macrophages to elicit protective immunity against lm infection. dcs themselves were inefficient at taking up lm but capable of taking up microparticles (mps) released by lm-infected macrophages. these mps transferred lm ... | 2012 | 23064105 |
| recent developments in molecular sub-typing of listeria monocytogenes. | as a vast majority of the human listeriosis cases are caused by serotypes 1/2a, 1/2b and 4b strains, it is imperative that strains from clinical as well as from food and environment are further characterised so that accurate and timely epidemiological determination of sources of the contamination can be established to minimise the disease burden. recent developments in the field of genomics provide a great opportunity to use these tools towards the development of molecular sub-typing techniques ... | 2013 | 23061558 |
| discriminating experimental listeria monocytogenes infections in mice using serum profiling. | serum profiling was used to distinguish mice infected with wild-type or mutant listeria monocytogenes from noninfected control mice. identifications of significant electrospray ionization mass spectrometry (esi-ms) sera peak areas between listeria-infected- and control mice were performed using t tests. esi-ms cohort peak distributions differed from mice infected with wild-type or ∆acta listeria versus control mice with p values of 0.00012 and 0.015, respectively. a "% wild-type listeria peaks i ... | 2012 | 23053068 |
| the effect of high hydrostatic pressure, sodium nitrite and salt concentration on the growth of listeria monocytogenes on rte ham and turkey. | growth of listeria monocytogenes was evaluated for up to 182 days after inoculation on ready-to-eat (rte) sliced ham and turkey breast formulated with sodium nitrite (0 or 200 ppm), sodium chloride (1.8% or 2.4%), and treated (no treatment or 600 mpa) with high hydrostatic pressure (hhp). hhp at 600 mpa for 3 min resulted in a 3.85-4.35 log cfu/g reduction in l. monocytogenes. with formulations at similar proximate analyses, one of the evaluation days (day 21) without hhp showed significantly gr ... | 2013 | 23046896 |
| spray method for recovery of heat-injured salmonella typhimurium and listeria monocytogenes. | selective agar is inadequate for supporting recovery of injured cells. during risk assessment of certain foods, both injured and noninjured cells must be enumerated. in this study, a new method (agar spray method) for recovering sublethally heat-injured microorganisms was developed and used for recovery of heat-injured salmonella typhimurium and listeria monocytogenes. molten selective agar was applied as an overlay to presolidified nonselective tryptic soy agar (tsa) by spray application. heat- ... | 2012 | 23043840 |
| immune status and the development of listeria monocytogenes infection in aged and young guinea pigs. | consuming even low numbers of the foodborne pathogen, listeria monocytogenes, places the elderly at risk for severe illness. the impact of immunomodulation on the development of listerial infection within a young and aged population after low dose challenge with l. monocytogenes was investigated. | 2012 | 23043712 |
| hydrodynamic shear stress to remove listeria monocytogenes biofilms from stainless steel and polytetrafluoroethylene surfaces. | to calculate the shear stress needed to remove sessile listeria monocytogenes cells from stainless steel (ss) and polytetrafluoroethylene (ptfe) surfaces. | 2013 | 23043564 |
| silage collected from dairy farms harbors an abundance of listeriaphages with considerable host range and genome size diversity. | since the food-borne pathogen listeria monocytogenes is common in dairy farm environments, it is likely that phages infecting this bacterium ("listeriaphages") are abundant on dairy farms. to better understand the ecology and diversity of listeriaphages on dairy farms and to develop a diverse phage collection for further studies, silage samples collected on two dairy farms were screened for l. monocytogenes and listeriaphages. while only 4.5% of silage samples tested positive for l. monocytogene ... | 2012 | 23042180 |
| usefulness of the european epidemic intelligence information system in the management of an outbreak of listeriosis, belgium, 2011. | a cluster of time-linked cases and the identification of a clonal strain suggest the occurrence of an outbreak of listeriosis in belgium in 2011, presumably due to the consumption of hard cheese made with pasteurised milk and produced by a belgium manufacturer. the outbreak clone was identified as listeria monocytogenes serovar 1/2a, sensitive to arsenic and cadmium and of multilocus sequence typing mlst-type 37. food investigation of this outbreak was facilitated by the european epidemic intell ... | 2012 | 23040965 |
| listeriosis in pregnancy: case report and retrospective study. | listeria monocytogenes usually causes mild maternal illness, but can be devastating to the fetus. | 2013 | 23039339 |
| histone acetylation mediates epigenetic regulation of transcriptional reprogramming in insects during metamorphosis, wounding and infection. | | 2012 | 23035888 |
| effect of x-ray treatments on pathogenic bacteria, inherent microbiota, color, and texture on parsley leaves. | this work is a part of systematic studies of the effect of x-ray treatments on fresh produce. the main objective of this investigation was to study the effects of x-ray treatments in reducing the concentration of artificially inoculated escherichia coli o157:h7, listeria monocytogenes, salmonella enterica, and shigella flexneri, and inherent microbiota on parsley leaves. the secondary objective was to study the effects of x-ray treatments on color and texture parameters on treated parsley leaves ... | 2012 | 23035726 |
| antimicrobial properties of three lactic acid bacterial cultures and their cell free supernatants against listeria monocytogenes. | control of listeria monocytogenes in ready-to-eat (rte) food products is a significant challenge and improved means for control are needed. in this study, the anti-listerial effects of three lactic acid bacteria (lab) were investigated. spot-on-lawn assays demonstrated the largest zones of inhibition against l. monocytogenes were produced by the pediococcus acidilactici strain, with zone diameters ranging from 13 to 18 mm. minimum inhibitory concentration (mic) experiments using cell free supern ... | 2013 | 23030442 |
| 4-1bb signaling breaks the tolerance of maternal cd8+ t cells that are reactive with alloantigens. | 4-1bb (cd137, tnfrsf9), a member of the activation-induced tumor necrosis factor receptor family, is a powerful t-cell costimulatory molecule. it generally enhances cd8(+) t responses and even breaks the tolerance of cd8(+) t cells in an antigen-specific manner. in the present study we found that it was expressed in the placentas of pregnant mice and that its expression coincided with that of the immunesuppressive enzyme indoleamine 2,3-dioxygenase (ido). therefore, we investigated whether 4-1bb ... | 2012 | 23029041 |
| distinct licensing of il-18 and il-1β secretion in response to nlrp3 inflammasome activation. | inflammasome activation permits processing of interleukins (il)-1β and 18 and elicits cell death (pyroptosis). whether these responses are independently licensed or are "hard-wired" consequences of caspase-1 (casp1) activity has not been clear. here, we show that that each of these responses is independently regulated following activation of nlrp3 inflammasomes by a "non-canonical" stimulus, the secreted listeria monocytogenes (lm) p60 protein. primed murine dendritic cells (dcs) responded to p6 ... | 2012 | 23028835 |
| infection-related declines in chill coma recovery and negative geotaxis in drosophila melanogaster. | studies of infection in drosophila melanogaster provide insight into both mechanisms of host resistance and tolerance of pathogens. however, research into the pathways involved in these processes has been limited by the relatively few metrics that can be used to measure sickness and health throughout the course of infection. here we report measurements of infection-related declines in flies' performance on two different behavioral assays. d. melanogaster are slower to recover from a chill-induce ... | 2012 | 23028430 |
| insights into cell division using listeria monocytogenes infections of ptk2 renal epithelial cells. | the assembly of actin into a cleavage furrow is accompanied by disassembly of the interphase actin cytoskeleton. a variation of this actin filament disassembly/assembly cycle is seen during cell division in ptk2 cells infected with the intracellular pathogen, listeria monocytogenes, where f-actin associates with the bacteria either as a halo surrounding nonmoving bacteria, or as an array of filaments that encases the sides of moving baceteria and extends behind them like a tail. the moving liste ... | 2012 | 23027717 |
| listeria monocytogenes differential transcriptome analysis reveals temperature-dependent agr regulation and suggests overlaps with other regulons. | listeria monocytogenes is a ubiquitous, opportunistic pathogenic organism. environmental adaptation requires constant regulation of gene expression. among transcriptional regulators, agra is part of an auto-induction system. temperature is an environmental cue critical for in vivo adaptation. in order to investigate how temperature may affect agra-dependent transcription, we compared the transcriptomes of the parental strain l. monocytogenes egd-e and its δagra mutant at the saprophytic temperat ... | 2012 | 23024744 |
| nanocapsular dispersion of thymol for enhanced dispersibility and increased antimicrobial effectiveness against escherichia coli o157:h7 and listeria monocytogenes in model food systems. | essential oils are marginally soluble in water, making it challenging to evenly disperse them in foods and resulting in an increased tendency to bind with food lipids and proteins, resulting in lowered antimicrobial efficacy. in the current study, free and nano-dispersed (nd) thymol were compared in terms of their antimicrobial efficacies against escherichia coli o157:h7 atcc 43889 and 43894 and listeria monocytogenes strains scott a and 101 in apple cider and 2% reduced-fat milk. apple cider wa ... | 2012 | 23023745 |
| acute cholecystitis with listeria monocytogenes. | listeriosis, an opportunistic food-borne disease caused by listeria monocytogenes, is infrequent and occurs preferentially in patients at the extremes of age, during pregnancy or in immunocompromised hosts. most common manifestations are maternofoetal and neonatal infections, severe invasive presentations such as bacteraemia with or without central nervous system symptoms occuring preferentially in immunosuppressed patients and self-limited gastro-enteritis affecting healthy individuals. excepti ... | 2016 | 23019807 |
| tetrameric assembly of kvlm k+ channels with defined numbers of voltage sensors. | voltage-gated k(+) (kv) channels are tetrameric assemblies in which each modular subunit consists of a voltage sensor and a pore domain. kvlm, the voltage-gated k(+) channel from listeria monocytogenes, differs from other kv channels in that its voltage sensor contains only three out of the eight charged residues previously implicated in voltage gating. here, we ask how many sensors are required to produce a functional kv channel by investigating heterotetramers comprising combinations of full-l ... | 2012 | 23019583 |
| transient enhanced il-2r signaling early during priming rapidly amplifies development of functional cd8+ t effector-memory cells. | much is known concerning the cellular and molecular basis for cd8(+) t memory immune responses. nevertheless, conditions that selectively support memory generation have remained elusive. in this study, we show that an immunization regimen that delivers tcr signals through a defined antigenic peptide, inflammatory signals through lps, and growth and differentiation signals through the il-2r initially favors ag-specific cd8(+) t cells to develop rapidly and substantially into t effector-memory cel ... | 2012 | 23018461 |
| impact of ionic strength of growth on the physiochemical properties, structure, and adhesion of listeria monocytogenes polyelectrolyte brushes to a silicon nitride surface in water. | the adhesion energies between pathogenic listeria monocytogenes egde to a model surface of silicon nitride were quantified using atomic force microscopy (afm) in water for cells grown in pure media (as the control) and in media of four different ionic strengths of added nacl (is of 0.05 m, 0.1 m, 0.3 m and 0.5 m nacl). the physiochemical properties of l. monocytogenes egde surface brushes were shown to have a strong influence on the adhesion of the microbe to the silicon nitride surface. the tra ... | 2012 | 23010316 |
| development of memory cd8+ t cells and their recall responses during blood-stage infection with plasmodium berghei anka. | conditions required for establishing protective immune memory vary depending on the infecting microbe. although the memory immune response against malaria infection is generally thought to be relatively slow to develop and can be lost rapidly, experimental evidence is insufficient. in this report, we investigated the generation, maintenance, and recall responses of ag-specific memory cd8(+) t cells using plasmodium berghei anka expressing ova (pba-ova) as a model system. mice were transferred wi ... | 2012 | 23008449 |
| role for telomerase in listeria monocytogenes infection. | human telomerase reverse transcriptase (htert) is the catalytic subunit of the human telomerase complex. growing evidence suggests that htert also contributes to the cell physiology independently of telomere elongation. however, its role in bacterial infection is unknown. here we show that htert is critical for listeria monocytogenes infection, as the depletion of htert impaired bacterial intracellular replication. in addition, we observed that l. monocytogenes caused a decrease in htert levels ... | 2012 | 23006849 |
| inducible renitence limits listeria monocytogenes escape from vacuoles in macrophages. | membranes of endolysosomal compartments in macrophages are often damaged by physical or chemical effects of particles ingested through phagocytosis or by toxins secreted by intracellular pathogens. this study identified a novel inducible activity in macrophages that increases resistance of phagosomes, late endosomes, and lysosomes to membrane damage. pretreatment of murine macrophages with lps, peptidoglycan, tnf-α, or ifn-γ conferred protection against subsequent damage to intracellular membran ... | 2012 | 23002437 |
| wall teichoic acids restrict access of bacteriophage endolysin ply118, ply511, and plyp40 cell wall binding domains to the listeria monocytogenes peptidoglycan. | the c-terminal cell wall binding domains (cbds) of phage endolysins direct the enzymes to their binding ligands on the bacterial cell wall with high affinity and specificity. the listeria monocytogenes ply118, ply511, and plyp40 endolysins feature related cbds which recognize the directly cross-linked peptidoglycan backbone structure of listeria. however, decoration with fluorescently labeled cbds primarily occurs at the poles and septal regions of the rod-shaped cells. to elucidate the potentia ... | 2012 | 23002226 |
| a combined use of autolysin p60 and listeriolysin o antigens induces high protective immune responses against listeria monocytogenes infection. | listeria monocytogenes is an intracellular bacterium responsible for listeriosis in both humans and animals. infected livestock is believed to be one source of this pathogen. vaccination is an optimal approach to control the occurrence of this disease in livestock. however, inactivated vaccines have been reported to be insufficient to offer immune protection against l. monocytogenes. here we evaluated the immune protection capacity of a combination of recombinant p60 and llo. mice immunized with ... | 2012 | 23001425 |
| exendin-4 improves resistance to listeria monocytogenes infection in diabetic db/db mice. | the incidence of diabetes mellitus is increasing among companion animals. this disease has similar characteristics in both humans and animals. diabetes is frequently identified as an independent risk factor for infections associated with increased mortality. in the present study, homozygous diabetic (db/db) mice were infected with listeria (l.) monocytogenes and then treated with the anti-diabetic drug exendin-4, a glucagon-like peptide 1 analogue. in aged db/db mice, decreased cd11b(+) macropha ... | 2012 | 23000581 |
| [occurrence and typing of listeria monocytogenes isolated from raw cow's milk collected on farms and from vending machines]. | evaluation of the incidence and characteristics of l. monocytogenes in samples of raw cow's milk collected on farms (bulk tank milk samples) and from vending machines. | 2012 | 22997776 |
| hospital-acquired listeriosis outbreak caused by contaminated diced celery--texas, 2010. | listeria monocytogenes causes often-fatal infections affecting mainly immunocompromised persons. sources of hospital-acquired listeriosis outbreaks can be difficult to identify. we investigated a listeriosis outbreak spanning 7 months and involving 5 hospitals. | 2013 | 22997210 |
| antibacterial soybean-oil-based cationic polyurethane coatings prepared from different amino polyols. | antibacterial soybean-oil-based cationic polyurethane (pu) coatings have been successfully prepared from five different amino polyols. the structure and hydroxyl functionality of these amino polyols affects the particle morphology, mechanical properties, thermal stability, and antibacterial properties of the resulting coatings. an increase in the hydroxyl functionality of the amino polyols increases the cross-link density, resulting in an increased glass transition temperature and improved mecha ... | 2012 | 22997044 |
| bacteriophage p70: unique morphology and unrelatedness to other listeria bacteriophages. | listeria monocytogenes is an important food-borne pathogen, and its bacteriophages find many uses in detection and biocontrol of its host. the novel broad-host-range virulent phage p70 has a unique morphology with an elongated capsid. its genome sequence was determined by a hybrid sequencing strategy employing sanger and pacbio techniques. the p70 genome contains 67,170 bp and 119 open reading frames (orfs). our analyses suggest that p70 represents an archetype of virus unrelated to other known ... | 2012 | 22993158 |
| listeria monocytogenes in retailed raw chicken meat in malaysia. | this study aimed to determine the prevalence listeria monocytogenes in raw chicken meat samples at hypermarkets and wet markets. chicken drumsticks, breasts, and thighs were randomly selected. the most probable number (mpn) pcr method was used to quantify the l. monocytogenes in the samples. listeria monocytogenes was detected in 20% of the samples. occurrence of l. monocytogenes was highest in breast (42.03%) followed by drumstick (11.27%) and thigh (7.14%). samples from hypermarkets showed hig ... | 2012 | 22991558 |
| quantitative real-time pcr method with internal amplification control to quantify cyclopiazonic acid producing molds in foods. | a quantitative taqman real-time pcr (qpcr) method that includes an internal amplification control (iac) to quantify cyclopiazonic acid (cpa)-producing molds in foods has been developed. a specific primer pair (dmatf/dmatr) and a taqman probe (dmatp) were designed on the basis of dmat gene which encodes the enzyme dimethylallyl tryptophan synthase involved in the biosynthesis of cpa. the iac consisted of a 105 bp chimeric dna fragment containing a region of the hly gene of listeria monocytogenes. ... | 2012 | 22986206 |
| control of listeria monocytogenes and spoilage bacteria on smoked salmon during storage at 5 °c after x-ray irradiation. | in this study, smoked salmon fillets were artificially inoculated with listeria monocytogenes (3.7 ± 0.2 log cfu g(-1)) and treated with x-ray irradiation generated by a rs 2400 x-ray machine (rad source technologies inc.) using doses of 0.0, 0.1, 0.5, 1.0, and 2.0 kgy. unirradiated and irradiated samples were then stored at 5 °c for 35 days and tested for l. monocytogenes count after 0, 5, 10, 15, 20, 25, 30, and 35 days. also, uninoculated-untreated and uninoculated-treated samples with the lo ... | 2012 | 22986195 |
| a common clathrin-mediated machinery co-ordinates cell-cell adhesion and bacterial internalization. | invasive bacterial pathogens often target cellular proteins involved in adhesion as a first event during infection. for example, listeria monocytogenes uses the bacterial protein inla to interact with e-cadherin, hijack the host adherens junction (aj) machinery and invade non-phagocytic cells by a clathrin-dependent mechanism. here, we investigate a potential role for clathrin in cell-cell adhesion. we observed that the initial steps of aj formation trigger the phosphorylation of clathrin, and i ... | 2012 | 22984946 |
| modeling the growth of listeria monocytogenes in soft blue-white cheese. | the aim of this study was to develop a predictive model simulating growth over time of the pathogenic bacterium listeria monocytogenes in a soft blue-white cheese. the physicochemical properties in a matrix such as cheese are essential controlling factors influencing the growth of l. monocytogenes. we developed a predictive tertiary model of the bacterial growth of l. monocytogenes as a function of temperature, ph, nacl, and lactic acid. we measured the variations over time of the physicochemica ... | 2012 | 22983971 |
| the exclusive use of flow cytometry to evaluate the antibiotic-susceptibility. | live and injured bacteria cannot be successfully discriminated using flow cytometric methods (fcm) with commercial live/dead staining agents because injured cells have intact cell membranes and are counted as live cells. we previously reported that photoactivated ethidium monoazide (ema) directly cleaves bacterial dna both in vivo and in vitro (microbiol. immunol. 51:763-775, 2007). | 2012 | 22982588 |
| adaptive acid tolerance response of listeria monocytogenes strains under planktonic and immobilized growth conditions. | the acid resistance of listeria monocytogenes was evaluated: (i) after short (shock) or long-term (adaptation during growth) exposure to reduced (5.5) or neutral (7.2) ph in a liquid (broth) medium or on a solid surface (agar), and (ii) after growth on the surface of ham and turkey slices or in homogenates of these products. three l. monocytogenes strains (serotypes 1/2a, 1/2b and 4b) were individually inoculated at: (i) 10(4)-10(5)cfu/ml in tryptic soy broth with 0.6% yeast extract (tsbye) or o ... | 2012 | 22980026 |
| reduction of listeria monocytogenes biofilms on stainless steel and polystyrene surfaces by essential oils. | plant-derived essential oils were tested for their ability to eliminate biofilms of listeria monocytogenes on polystyrene and stainless steel surfaces. various concentrations of essential oils were tested with different contact times on biofilms of various ages. preliminarily screening of nine essential oils and related phenolic compounds in a disk diffusion assay revealed that thyme oil, oregano oil, and carvacrol had the highest antimicrobial activity. further screening of these three compound ... | 2012 | 22980020 |
| using nested pcr to detect the hlya gene of listeria monocytogenes in minas frescal cow's milk cheese. | to evaluate a nested pcr protocol for listeria monocytogenes detection, minas frescal cow's milk cheeses were produced, artificially inoculated with this pathogen at concentrations ranging from 1 to 1,000 cfu/g, and stored at 4°c for 10 days. the international organization for standardization (iso) standardized method 11290-1/a1 was used to detect l. monocytogenes in the inoculated samples, and dna was extracted from aliquots (1, 5, and 10 ml) of 1:10 dilution, followed by a nested pcr protocol ... | 2012 | 22980018 |
| health professionals' knowledge and understanding about listeria monocytogenes indicates a need for improved professional training. | listeria monocytogenes causes listeriosis, an uncommon but potentially fatal disease in immunocompromised persons, with a public health burden of approximately $2 billion annually. those consumers most at risk are the highly susceptible populations otherwise known as the immunocompromised. health professionals have a considerable amount of interaction with the immunocompromised and are therefore a valuable resource for providing appropriate safe food handling information. to determine how knowle ... | 2012 | 22980015 |
| annual cost of illness and quality-adjusted life year losses in the united states due to 14 foodborne pathogens. | in this article we estimate the annual cost of illness and quality-adjusted life year (qaly) loss in the united states caused by 14 of the 31 major foodborne pathogens reported on by scallan et al. (emerg. infect. dis. 17:7-15, 2011), based on their incidence estimates of foodborne illness in the united states. these 14 pathogens account for 95 % of illnesses and hospitalizations and 98 % of deaths due to identifiable pathogens estimated by scallan et al. we estimate that these 14 pathogens caus ... | 2012 | 22980013 |
| inhibitory effect of carob (ceratonia siliqua) leaves methanolic extract on listeria monocytogenes. | in recent years, there has been great development in the search for new natural compounds for food preservation aimed at a partial or total replacement of currently popular antimicrobial chemicals. carob (ceratonia siliqua) offers a natural promising alternative for food safety and bioconservation. in this work, the methanolic extract of carob leaves (mecl) was tested for the ability to inhibit the growth of a range of microorganisms. mecl inhibited the growth of listeria monocytogenes at 28.12 ... | 2012 | 22978382 |
| proteomic analysis of the response of listeria monocytogenes to bile salts under anaerobic conditions. | listeria monocytogenes is a food-borne pathogen responsible for the disease listeriosis. the infectious process depends on survival in the high bile-salt conditions encountered throughout the gastrointestinal tract, including the gallbladder. however, it is not clear how bile-salt resistance mechanisms are induced, especially under physiologically relevant conditions. this study sought to determine how the l. monocytogenes strains egde (serovar 1/2a), f2365 (serovar 4a) and hcc23 (serovar 4b) re ... | 2013 | 22977076 |
| the trpm2 ion channel, an oxidative stress and metabolic sensor regulating innate immunity and inflammation. | trpm2 (transient receptor potential melastatin 2) is the unique fusion of a ca(2+)-permeable pore with an enzymatic domain that binds the nad(+)-metabolite adp-ribose (adpr), resulting in channel opening. adpr formation is a metabolic corollary of cellular stress, but can also be elicited enzymatically through nad glycohydrolases like cd38. trpm2 thus functions as a metabolic and oxidative stress sensor and translates this information into ion fluxes that can affect ca(2+) signaling and the memb ... | 2013 | 22975787 |
| integrative genomic analysis identifies isoleucine and cody as regulators of listeria monocytogenes virulence. | intracellular bacterial pathogens are metabolically adapted to grow within mammalian cells. while these adaptations are fundamental to the ability to cause disease, we know little about the relationship between the pathogen's metabolism and virulence. here we used an integrative metabolic analysis tool that combines transcriptome data with genome-scale metabolic models to define the metabolic requirements of listeria monocytogenes during infection. twelve metabolic pathways were identified as di ... | 2012 | 22969433 |
| [clinical features and antimicrobial resistance of gram positive bacterial blood stream infection in patients with hematologic diseases]. | to study the clinical characteristics and antimicrobial resistance of bloodstream infections caused by gram positive bacteria, so as to provide reference for the rational use of antimicrobial agent. | 2012 | 22967420 |
| inflammatory monocyte recruitment is regulated by interleukin-23 during systemic bacterial infection. | listeria monocytogenes is a gram-positive intracellular pathogen that causes meningitis and septicemia in immunocompromised individuals and spontaneous abortion in pregnant women. the innate immune response against l. monocytogenes is primarily mediated by neutrophils and monocytes. interleukin-23 (il-23) is an important proinflammatory cytokine well known for its role in neutrophil recruitment in various infectious and autoimmune diseases. we have previously shown that il-23 is required for hos ... | 2012 | 22966045 |
| genetic diversity of internalin genes in the ascb-dape locus among listeria monocytogenes lineages iii and iv strains. | listeria monocytogenes is an important foodborne pathogen encompassing four phylogenetic lineages. lineages iii and iv are rare, but have been reported to show considerable biodiversity, providing important clues for the evolutionary history in listeria. in this study, analysis of the ascb-dape locus reveals genetic diversity in lineages iii and iv, and is consistent with the classification of sublineages. four of the six genetic patterns (two of sublineage iiic and two of lineage iv) are specif ... | 2013 | 22961731 |
| influence of planktonic and sessile listeria monocytogenes on caenorhabditis elegans. | listeria monocytogenes is the etiologic agent of listeriosis, a food-borne disease affecting humans and a variety of animals. in order to combat this pathogen, it is crucial to have an understanding of its natural interplay with the environment. for this reason, the free soil nematode caenorhabditis elegans was focused upon because of its shared natural habitat with listeria and its potential as a model organism for listeria pathogenesis. previous studies have generated some contradictory result ... | 2013 | 22961596 |
| homeostatic division is not necessary for antigen-specific cd4+ memory t cell persistence. | cd4(+) memory t cells are generated in response to infection or vaccination, provide protection to the host against reinfection, and persist through a combination of enhanced survival and slow homeostatic turnover. we used timed deletion of the tcr-signaling adaptor molecule src homology 2 domain-containing phosphoprotein of 76 kda (slp-76) with mhc:peptide tetramers to study the requirements for tonic tcr signals in the maintenance of polyclonal ag-specific cd4(+) memory t cells. slp-76-deficie ... | 2012 | 22956580 |
| myeloid cell-specific abca1 deletion protects mice from bacterial infection. | atp-binding cassette transporter a1 (abca1) plays a critical role in eliminating excess free cholesterol from tissues by effluxing cellular free cholesterol and phospholipids to lipid-poor apolipoprotein ai. macrophage abca1 also dampens proinflammatory myeloid differentiation primary-response protein 88-dependent toll-like receptor signaling by reducing cellular membrane free cholesterol and lipid raft content, indicating a role of abca1 in innate immunity. however, whether abca1 expression has ... | 2012 | 22955730 |
| phenotype and function of protective, cd4-independent cd8 t cell memory. | while the need for cd4 t cells in the generation of cd8 t cell memory has been well documented, the mechanism underlying their requirement remains unknown. here, we detail an immunization method capable of generating cd8 memory t cells that are indifferent to cd4 t cell help. using a subunit vaccination that combines polyic and an agonistic cd40 antibody, we program protective cd4-independent cd8 t cell memory. when cells generated by combined polyic/cd40 immunization are compared to cells produ ... | 2013 | 22948808 |
| effect of curing method and freeze-thawing on subsequent growth of listeria monocytogenes on cold-smoked salmon. | the presence of the foodborne pathogen listeria monocytogenes on cold-smoked salmon is a major concern for the seafood industry. understanding processing and postprocessing handling factors that affect the ability of this pathogen to grow on cold-smoked salmon is critical for developing effective control strategies. in this study, we investigated the effect of curing method and freeze-thawing of cold-smoked salmon on (i) physicochemical properties and (ii) subsequent growth of genetically divers ... | 2012 | 22947469 |
| filter-based pathogen enrichment technology for detection of multiple viable foodborne pathogens in 1 day. | conventional foodborne pathogen assays currently used in the food industry often require long culture enrichments to increase pathogen levels so they can be detected. even using sensitive real-time pcr assays, culture enrichment at least overnight is necessary especially for detection of pathogens with slow growth rates such as listeria monocytogenes. to eliminate this cumbersome enrichment step and detect minute amounts of pathogens within 1 day, filter-based pathogen enrichment technology was ... | 2012 | 22947467 |
| expression of efflux pump gene lde in ciprofloxacin-resistant foodborne isolates of listeria monocytogenes. | the expression of efflux pump gene lde in ciprofloxacin resistant (cip(r) ) and susceptible strains of listeria monocytogenes collected from retail food samples was investigated. for two cip(r) strains, the mics of ciprofloxacin decreased four- to eightfold in the presence of reserpine; however, no significant alterations were observed with naturally sensitive isolates. overexpression of the lde gene induced by ciprofloxacin was observed in two resistant isolates. the present findings indicate t ... | 2012 | 22943162 |
| [contribution of l,d-carboxypeptidases in virulence of facultative intracellular pathogenic bacteria listeria monocytogenes]. | evaluate influence of mutation of listeria monocytogenes genes coding murein-tetrapeptide l,d-carboxypeptidase lmo0028 and lmo1638 on dynamics of infectious process and interaction of purified muropeptides with nod1 receptor. | 2012 | 22937699 |
| marginal zone b cell is a major source of il-10 in listeria monocytogenes susceptibility. | rag-1-knockout (ko) mice are highly resistant to listeria monocytogenes infection. the role played by the many rag-1-dependent lymphocyte lineages was studied using a genetic approach in which each rag-1-dependent lymphocyte lineage was eliminated one at a time. only b cell-deficient igh-ko mice displayed reduced bacterial load and improved survival upon listeria infection. listeria infection of rag-1-ko and il-10-ko hosts that had been adoptively transferred with wild-type marginal zone b (mzb) ... | 2012 | 22933629 |
| rhamnose-inducible gene expression in listeria monocytogenes. | acid production from rhamnose is a characteristic phenotype of listeria monocytogenes. we report the identification of the rhamnose transport and utilization operon located at lmo2846 to lmo2851, including the rhamnose-dependent promoter p(rha). expression of reporter genes under control of p(rha) on a single copy integration vector demonstrated its suitability for inducible gene expression in l. monocytogenes. transcription initiation from p(rha) is dose dependent, and a concentration as low as ... | 2012 | 22927968 |
| subinhibitory concentrations of antibiotics affect stress and virulence gene expression in listeria monocytogenes and cause enhanced stress sensitivity but do not affect caco-2 cell invasion. | antibiotics can act as signal molecules and affect bacterial gene expression, physiology and virulence. the purpose of this study was to determine whether subinhibitory antibiotic concentrations alter gene expression and physiology of listeria monocytogenes. | 2012 | 22924966 |
| unveiling the expression characteristics of ispc, a cell wall-associated peptidoglycan hydrolase in listeria monocytogenes, during growth under stress conditions. | listeria monocytogenes serotype 4b is a food-borne pathogen of public health concern, since it accounts for approximately 40% of human listeriosis cases. we have recently identified ispc, a surface-localized peptidoglycan hydrolase, as the antigen recognized by a number of monoclonal antibodies (mabs) produced against a serotype 4b strain for diagnostic applications. to determine whether ispc, which is well conserved among various serotype 4b strains, is a useful diagnostic marker in antibody-ba ... | 2012 | 22923393 |
| listeria monocytogenes cytoplasmic entry induces fetal wastage by disrupting maternal foxp3+ regulatory t cell-sustained fetal tolerance. | although the intracellular bacterium listeria monocytogenes has an established predilection for disseminated infection during pregnancy that often results in spontaneous abortion or stillbirth, the specific host-pathogen interaction that dictates these disastrous complications remain incompletely defined. herein, we demonstrate systemic maternal listeria infection during pregnancy fractures fetal tolerance and triggers fetal wastage in a dose-dependent fashion. listeria was recovered from the ma ... | 2012 | 22916020 |
| pp2a-dependent control of transcriptionally active foxo3a in cd8(+) central memory lymphocyte survival requires p47(phox). | forkhead box o3a (foxo3a) transcription factor is regulated by complex post-translational modifications that allow for transcriptional control of various apoptosis factors including pro-apoptotic bim. although it has been shown that kinases phosphorylate foxo3a in memory t cells, the role of protein phosphatases in the control of memory t lymphocyte foxo3a function is less clear. here, we report that foxo3a is dephosphorylated (activated) by a protein phosphatase 2a (pp2a)-dependent mechanism in ... | 2012 | 22914323 |
| microarray-based transcriptome of listeria monocytogenes adapted to sublethal concentrations of acetic acid, lactic acid, and hydrochloric acid. | listeria monocytogenes , an important foodborne pathogen, commonly encounters organic acids in food-related environments. the transcriptome of l. monocytogenes l502 was analyzed after adaptation to ph 5 in the presence of acetic acid, lactic acid, or hydrochloric acid (hcl) at 25 °c, representing a condition encountered in mildly acidic ready-to-eat food kept at room temperature. the acid-treated cells were compared with a reference culture with a ph of 6.7 at the time of rna harvesting. the num ... | 2012 | 22913877 |
| subcellular localization of extracytoplasmic proteins in monoderm bacteria: rational secretomics-based strategy for genomic and proteomic analyses. | genome-scale prediction of subcellular localization (scl) is not only useful for inferring protein function but also for supporting proteomic data. in line with the secretome concept, a rational and original analytical strategy mimicking the secretion steps that determine ultimate scl was developed for gram-positive (monoderm) bacteria. based on the biology of protein secretion, a flowchart and decision trees were designed considering (i) membrane targeting, (ii) protein secretion systems, (iii) ... | 2012 | 22912771 |
| effect of liquid smoke as an ingredient in frankfurters on listeria monocytogenes and quality attributes. | market trends indicate an increased interest in natural antimicrobials to augment safety of ready-to-eat meat and poultry products against listeria monocytogenes. liquid smoke, an all-natural condensate of smoke components, applied as a postprocess treatment on product surface has the potential to exhibit antilisterial properties. studies on its antimicrobial efficacy and quality attributes as an ingredient are not sufficient. a study was designed to validate the antimicrobial effect of liquid s ... | 2012 | 22912472 |