| a rapid method for authentication of buffalo (bubalus bubalis) meat by alkaline lysis method of dna extraction and species specific polymerase chain reaction. | buffalo (bubalus bubalis) meat is a major item of export from india but export of beef i.e. meat from cattle (bos indicus) is prohibited. also, adulteration of buffalo meat with that of beef (meat from cattle) is a common fraudulent practice because of prohibition on cow slaughter in most states of india. food analysts require precise identification techniques to implement such regulations. in the present study, a method of dna extraction by alkaline lysis from meat samples and speciation of buf ... | 2011 | 24425899 |
| proteomic based approach for characterizing 4-hydroxy-2-nonenal induced oxidation of buffalo (bubalus bubalis) and goat (capra hircus) meat myoglobins. | myoglobin (mb) is a sarcoplasmic heme protein primarily responsible for meat color and its chemistry is species specific. 4-hydroxy-2-nonenal (hne) is a cytotoxic lipid derived aldehyde detected in meat and was reported to covalently adduct with nucleophilic histidine residues of mb and predispose it to greater oxidation. however, no literature is available on characterization of lipid oxidation induced oxidation of indian water buffalo (bubalus bubalis) and goat (capra hircus) myoglobins. | 2016 | 27891064 |
| infection of water buffalo in rio de janeiro brazil with anaplasma marginale strains also reported in cattle. | anaplasma marginale is the most prevalent pathogen of cattle in tropical and subtropical regions of the world and causes the disease bovine anaplasmosis. the importance of water buffalo in the world economy is increasing. in addition, while water buffalo may serve as a reservoir host for a. marginale, the susceptibility of this host for a. marginale cattle strains in brazil has not been reported. the major surface protein 1 alpha (msp1α) gene has been shown to be a stable genetic marker for iden ... | 2014 | 25260335 |
| a report on the high prevalence of anaplasma sp. in buffaloes from two provinces in pakistan. | the present study was designed to use a pcr-rflp protocol for the molecular detection of anaplasma sp. and to compare its prevalence in blood samples from buffaloes (bubalus bubalis) from 2 provinces of pakistan and to determine the risk factors associated with the spread of anaplasma infection. a total of 281 blood samples were collected from adults and calves of buffaloes from 4 sampling sites in southern punjab (bahawalnagar, burewala, layyah, and multan districts) and 2 in khyber pukhtoon kh ... | 2013 | 23743023 |
| short communication: arcobacter butzleri and arcobacter cryaerophilus survival and growth in artisanal and industrial ricotta cheese. | ricotta cheese is a ready-to-eat product with properties (ph >6.0, aw >0.98-0.99) and moisture content (75-80%) that may pose a risk to public health due to postprocess contamination by several bacterial pathogens, including arcobacters. the objective of the study was to evaluate the behavior of arcobacter butzleri and arcobacter cryaerophilus in ricotta cheese during its shelf life assuming postprocessing contamination. two types of ricotta cheese, artisanal water buffalo (wb) and industrial co ... | 2015 | 26233450 |
| prevalence and antimicrobial susceptibility of arcobacter species in cow milk, water buffalo milk and fresh village cheese. | in this study, the presence of arcobacter spp. was examined in cow milk (n=50), water buffalo (wb) milk (n=50) and fresh village cheese (n=50) samples. the 16s rdna-rflp method was used for the identification of arcobacter spp. the disc diffusion method was used to investigate the susceptibility of all strains identified to 18 different antimicrobial substances. the most commonly isolated arcobacter species were found to be arcobacter butzleri (38.89%), arcobacter cryaerophilus (22.23%) and arco ... | 2014 | 25064812 |
| survival of arcobacter butzleri during production and storage of artisan water buffalo mozzarella cheese. | water buffalo mozzarella cheese (wbmc) is a fresh stretched cheese produced from whole chilled buffalo milk. although pasteurization of milk and the use of defined starter cultures are recommended, traditional technology involving unpasteurized milk and natural whey cultures is still employed for wbmc production in italy. the purpose of this study was to assess the behavior of arcobacter butzleri during wbmc production and storage under different temperature conditions (5, 10, and 20 °c). raw mi ... | 2013 | 23806147 |
| presence of campylobacter and arcobacter species in in-line milk filters of farms authorized to produce and sell raw milk and of a water buffalo dairy farm in italy. | the objectives of this study were to investigate the presence of campylobacter spp. and arcobacter spp. in dairy herds authorized for the production and sale of raw milk and in a water buffalo dairy farm, and to test the antimicrobial susceptibility of the isolates. a total of 196 in-line milk filters were collected from 14 dairy farms (13 bovine and 1 water buffalo) for detection of campylobacter spp. and arcobacter spp. by microbiological culture. for each farm investigated, 1 isolate for each ... | 2013 | 23453517 |
| molecular assays reveal the presence of theileria spp. and babesia spp. in asian water buffaloes (bubalus bubalis, linnaeus, 1758) in the amazon region of brazil. | approximately 50% of buffalo herds in brazil are located in pará state in northern brazil. there are several properties where cattle and buffalo live and graze together, and thus, buffalo pathogens may threaten the health of cattle and vice versa. therefore, knowledge of infectious agents of buffalo is essential for maintaining healthy livestock. clinical disease caused by theileria and babesia parasites in the asian water buffalo is not common, although these animals may act as reservoir hosts, ... | 2016 | 27344507 |
| epidemiology of bovine hemoprotozoa parasites in cattle and water buffalo in vietnam. | a pcr-based survey of hemoprotozoa parasites detected babesia bigemina, theileria orientalis and trypanosoma theileri among cattle and water buffalo in vietnam, and a new babesia sp. closely related to babesia ovata was detected in cattle only. in addition, theileria annulata and trypanosoma evansi were not detected in both cattle and water buffalo. phylogenetic analysis detected t. orientalis mpsp genotypes 3, 5, 7 and n3 in cattle and 5, 7, n1 and n2 in water buffalo. additionally, water buffa ... | 2016 | 27149894 |
| diagnostic value of the recombinant tandem repeat antigen tegm6-4r for surra in water buffaloes. | trypanosoma evansi infection, or surra, is currently affecting various species of animals, especially water buffaloes. since diagnosis is an important aspect of surra control, development of novel diagnostic antigens is of interest to implement and improve the currently utilized methods. our study evaluated the tandem repeat antigen tegm6-4r in t. evansi antibody detection in water buffaloes. tegm6-4r-based elisa was performed with 20 positive and 8 negative controls and 484 field samples from w ... | 2014 | 24524896 |
| genetic characterization of babesia and theileria parasites in water buffaloes in sri lanka. | water buffaloes are thought to be the reservoir hosts for several hemoprotozoan parasites that infect cattle. in the present study, we surveyed sri lankan bred water buffaloes for infections with babesia bovis, babesia bigemina, theileria annulata, and theileria orientalis using parasite-specific pcr assays. when 320 blood-derived dna samples from water buffaloes reared in three different districts (polonnaruwa, mannar, and mullaitivu) of sri lanka were pcr screened, b. bovis, b. bigemina, and t ... | 2014 | 24365246 |
| the apicoplast genomes of two taxonomic units of babesia from sheep. | the apicoplast (ap) is a unique, non-photosynthetic organelle found in most apicomplexan parasites. due to the essential roles that this organelle has, it has been widely considered as target for drugs against diseases caused by apicomplexans. exploring the ap genomes of such parasites would provide a better understanding of their systematics and their basic molecular biology for therapeutics. however, there is limited information available on the ap genomes of apicomplexan parasites. in the pre ... | 2017 | 27916258 |
| characterization and annotation of babesia orientalis apicoplast genome. | babesia orientalis is an obligate intraerythrocytic protozoan parasite of the buffalo (bubalus bubalis, linnaeus, 1758) transmitted by the tick rhipicephalus heamaphysaloides. it is the causative agent of water buffalo babesiosis, one of the most important pathogens of water buffalo in central and southern china. as a member of the phylum apicomplexa, b. orientalis possesses a relatively independent and alga originated organelle the apicoplast. apicoplasts in other apicomplexa parasites are invo ... | 2015 | 26474853 |
| identification and characterization of a novel 34 kda merozoite protein in babesia orientalis. | a novel babesia orientalis 34 kda protein (designated bop34) was obtained by immunoscreening of a cdna expression library using b. orientalis infected water buffalo serum. the complete nucleotide sequence of the bop34 was 1088 bp, which contained one open reading frame (orf), two untranslated regions (utrs) and a poly (a) tail. the length of orf was 933 bp, encoding a polypeptide of 310 aa with a predicted size of 34 kda. blast analysis showed that the nucleotide sequence of bop34 had 71% simila ... | 2015 | 26169218 |
| genetic variations in merozoite surface antigen genes of babesia bovis detected in vietnamese cattle and water buffaloes. | the genes that encode merozoite surface antigens (msas) in babesia bovis are genetically diverse. in this study, we analyzed the genetic diversity of b. bovis msa-1, msa-2b, and msa-2c genes in vietnamese cattle and water buffaloes. blood dna samples from 258 cattle and 49 water buffaloes reared in the thua thien hue province of vietnam were screened with a b. bovis-specific diagnostic pcr assay. the b. bovis-positive dna samples (23 cattle and 16 water buffaloes) were then subjected to pcr assa ... | 2015 | 25575442 |
| molecular cloning and characterization of babesia orientalis rhoptry-associated protein 1. | the rhoptry-associated protein 1 (rap-1) gene of babesia orientalis was obtained from a cdna expression library by immunoscreening with b. orientalis-infected water buffalo sera. the nucleotide sequence of the cdna was 1732 bp with an open reading frame (orf) of 1434 bp, encoding a polypeptide of 478 amino acid residues with a predicted size of 52.5 kda. the orf was cloned into a pgex-kg plasmid and subsequently expressed as a gst-fusion protein. the recombinant rap-1 of b. orientalis (rborap-1) ... | 2014 | 25199690 |
| molecular cloning and characterization of a novel heat shock protein 20 of babesia orientalis. | the heat shock protein 20 (hsp20) gene of babesia orientalis (bohsp20) was identified from both genomic dna and cdna. the full-length bohsp20 gene was 690bp with one intron from position 88-243bp. the amplicon obtained from cdna corresponded to a full-length open reading frame (orf) with a length of 534bp, encoding a polypeptide of 178 amino acid residues with a predicted size of 20kda. the orf was cloned into a pet-28a plasmid and subsequently expressed as a his-fusion protein. the recombinant ... | 2014 | 24857770 |
| differential expression of th1- and th2- type cytokines in peripheral blood mononuclear cells of murrah buffalo (bubalus bubalis) on tlr2 induction by b. subtilis peptidoglycan. | peripheral blood mononuclear cells (pbmcs) discriminate microbial pathogens and induce t-cell responses of appropriate effector phenotype accordingly. toll-like receptors (tlrs), in part, mediate this microbial recognition and differentiation while the development of t-cell effector functions critically depends on the release of th1- or th2- type cytokines. in the present study, buffalo pbmcs were stimulated under in vitro culture conditions by bacillus subtilis cell wall petidoglycan, a tlr2 li ... | 2012 | 25049659 |
| evidence for bovine besnoitiosis in egypt-first serosurvey of besnoitia besnoiti in cattle and water buffalo (bubalus bubalis) in egypt. | the present study evaluated the presence of specific antibodies against besnoitia besnoiti in cattle and water buffalo (bubalus bubalis) in egypt. sera from cattle (n = 216) and water buffaloes (n = 133) collected from five different provinces of egypt (behera, alexandria, assuit, gharbia, and matrouh) were analyzed. testing for b. besnoiti antibodies by priocheck® besnoitia ab 2.0 elisa initially identified 13.75 % (48 out of 349) of individual sera as positive at the manufacturer's suggested c ... | 2014 | 24375275 |
| first genome sequences of buffalo coronavirus from water buffaloes in bangladesh. | we report the complete genome sequences of a buffalo coronavirus (bufcov hku26) detected from the faecal samples of two domestic water buffaloes (bubalus bubalis) in bangladesh. they possessed 98-99% nucleotide identities to bovine coronavirus (bcov) genomes, supporting bufcov hku26 as a member of betacoronavirus 1. nevertheless, bufcov hku26 possessed distinct accessory proteins between spike and envelope compared to bcov. sugar-binding residues in the n-terminal domain of s protein in bcov are ... | 2016 | 27274850 |
| a serosurvey for ruminant pestivirus exposure conducted using cattle sera collected for brucellosis surveillance in the united states. | four species of ruminant pestivirus are currently circulating in the united states: bovine viral diarrhea virus 1 and 2 (bvdv-1, -2; predominant host: cattle), border disease virus (bdv; predominant host: sheep), and pronghorn virus (sporadically detected in wild ruminants). a third bovine pestivirus called hobi-like virus has been detected in cattle and water buffalo in south america, asia, and europe. to date, no isolations of hobi-like viruses from u.s. cattle have been reported. to assess ex ... | 2017 | 28074709 |
| detection of anti-borrelia burgdorferi antibodies in buffaloes (bubalus bubalis) in the state of pará, brazil. | this study aimed to investigate the frequency of homologous antibodies of igg class against borrelia burgdorferi in buffaloes in the state of pará, brazil. blood serum samples from 491 buffaloes were analyzed by means of the indirect elisa test, using crude antigen produced from a cultivar of the north american strain g39/40 of b. burgdorferi. there were 412 positive samples (83.91%), and there was no statistically significant difference in the proportions of positive animals between the 81.69% ... | 2012 | 23070455 |
| expression and antigenic characterization of bubaline herpesvirus 1 (buhv1) glycoprotein e and its potential application in the epidemiology and control of alphaherpesvirus infections in mediterranean water buffalo. | bubaline herpesvirus 1 (buhv1) is a member of ruminant alphaherpesviruses antigenically related to bovine herpesvirus 1 (bohv1). the impact of buhv1 infection in infectious bovine rhinotracheitis control program is difficult to establish, due to the lack of specific diagnostic test. the ectodomain of glycoprotein e of buhv1 was expressed as recombinant secreted protein and used in indirect elisa as well as in a discriminatory test using the bohv1 counterpart. a panel of monoclonal antibodies was ... | 2014 | 24992670 |
| bovine herpesvirus type 1 marker vaccine induces cross-protection against bubaline herpesvirus type 1 in water buffalo. | water buffalo (bubalus bubalis) are susceptible to bovine herpesvirus type 1 (bohv-1) and a species-specific herpesvirus, bubaline herpesvirus type 1 (buhv-1). in this study, an attenuated marker bohv-1 based vaccine against buhv-1 challenge was evaluated to determine whether it induces protection from viral replication. one group of water buffalo calves was immunized with an attenuated bohv-1 marker vaccine. a second group was not vaccinated and used as the control. during the post-vaccination ... | 2014 | 24985155 |
| first report of isolation and molecular characterization of bubaline herpesvirus 1 (buhv1) from argentinean water buffaloes. | herpesviruses have mainly co-evolved with their hosts for millions of years. however, bovine herpesvirus 1 (bohv1) and related ruminant alphaherpesviruses have been reported to cross the species barrier. bubaline herpesvirus 1 (buhv1) is an alphaherpesvirus closely related to bohv1 and bohv5. according to the serological cross-relationships between ruminant alphaherpesviruses, several surveys have studied the occurrence of bohv1-related virus infection in wild and domestic ruminant species. rece ... | 2014 | 24938487 |
| genome sequence of bubaline alphaherpesvirus 1 (buhv1) isolated in australia in 1972. | bubaline alphaherpesvirus 1 (buhv1) is a member of the family herpesviridae, subfamily alphaherpesvirinae, genus varicellovirus. to date, no full genome sequence of buhv has been published. here, we report the complete genome sequence of bubaline alphaherpesvirus 1 (buhv1) strain b6 (buhv1-b6), isolated from a water buffalo (bubalus bubalis) in 1972 in australia. the virus was multiplied in mdbk cells, and the dna was extracted and subjected to high-throughput sequencing. the reads were aligned ... | 2017 | 28063080 |
| characterization of a recombinant newcastle disease virus expressing the glycoprotein of bovine ephemeral fever virus. | bovine ephemeral fever (bef) is caused by the arthropod-borne bovine ephemeral fever virus (befv), which is a member of the family rhabdoviridae and the genus ephemerovirus. befv causes an acute febrile infection in cattle and water buffalo. in this study, a recombinant newcastle disease virus (ndv) expressing the glycoprotein (g) of befv (rl-befv-g) was constructed, and its biological characteristics in vitro and in vivo, pathogenicity, and immune response in mice and cattle were evaluated. bef ... | 2017 | 27757685 |
| risk analysis and seroprevalence of bovine ephemeral fever virus in cattle in the kingdom of saudi arabia. | bovine ephemeral fever virus (befv) is an arthropod-borne rhabdovirus that causes disabling clinical signs and major economic losses in cattle and water buffalo. the disease is well documented in asia, africa, and the middle east; however, the seroprevalence of befv in different regions and bovine breeds in the kingdom of saudi arabia (ksa) is unknown. the aim of this study was to analyze risk factors which affect the prevalence of antibodies against befv in small herds of cattle in four geograp ... | 2016 | 26676243 |
| large-scale serological survey of bovine ephemeral fever in china. | bovine ephemeral fever (bef) is caused by the arthropod-borne bovine ephemeral fever virus (befv), which is classified in the family rhabdoviridae and the genus ephemerovirus. a debilitating and sometimes fatal viral disease, bef affects cattle and water buffalo. the epizootiology of bef among cattle in china has not been fully determined. we examined the seroprevalence of the befv among cattle in china between january 2012 and june 2014. among the 2822 serum samples collected from various cattl ... | 2015 | 25595266 |
| seroprevalence of bovine ephemeral fever virus in domesticated and wildlife species during epidemic and inter-epidemic periods (2000-2009) in israel. | bovine ephemeral fever (bef) is an economically important vector-borne viral disease of cattle and buffalo. it has been reported from most of the world's tropical and subtropical regions. in the last few decades, outbreaks of bef have occurred in israel almost every other year. several serological studies have demonstrated a wide range of wild animal species that are positive for bef virus (befv) antibodies. however, the question of whether wild animals and domesticated species other than cattle ... | 2015 | 23675891 |
| molecular detection of bovine immunodeficiency virus in water buffaloes (bubalus bubalis) from the amazon region, brazil. | bovine immunodeficiency is a chronic progressive disease caused by a lentivirus that affects cattle and buffaloes. although the infection has been described in cattle in some countries, including in brazil, there are only two reports of infection in buffaloes: one in pakistan and one in cambodia. the aim of the present study was to survey the occurrence of bovine immunodeficiency virus (biv) in water buffaloes from the amazon region, pará state, brazil. biv proviral dna was surveyed in 607 whole ... | 2015 | 26174574 |
| bovine papillomavirus type 2 infects the urinary bladder of water buffalo (bubalus bubalis) and plays a crucial role in bubaline urothelial carcinogenesis. | bovine papillomavirus type 2 (bpv-2) has been shown to infect and play a role in urinary bladder carcinogenesis of buffaloes grazed on pastures with ferns from the marmara and black sea regions of turkey. bpv-2 dna has been found in both neoplastic and non-neoplastic lesions of the urinary bladder. furthermore, this virus may be a normal inhabitant of the urinary bladder since bpv-2 dna has also been detected in clinically normal buffaloes. the viral activation by fern immunosuppressant or carci ... | 2013 | 23100367 |
| detection of pseudocowpox virus in water buffalo (bubalus bubalis) with vesicular disease in the state of são paulo, brazil, in 2016. | parapoxviruses are zoonotic viruses that infect cattle, goats and sheep; there have also been reports of infections in camels, domestic cats and seals. | 2017 | 27774853 |
| isolation and characterization of bovine parainfluenza virus type 3 from water buffaloes (bubalus bubalis) in argentina. | parainfluenza virus type 3 (piv3) was isolated from dairy buffaloes (bubalus bubalis) naturally affected with respiratory and reproductive clinical conditions. | 2012 | 22716217 |
| molecular analyses detect natural coinfection of water buffaloes (bubalus bubalis) with bovine viral diarrhea viruses (bvdv) in serologically negative animals. | infection of water buffaloes (bubalus bubalis) with bovine viral diarrhea viruses (bvdv) has been confirmed in several studies by serological and molecular techniques. in order to determine the presence of persistently infected animals and circulating species and subtypes of bvdv we conducted this study on a buffalo herd, whose habitat was shared with bovine cattle (bossp.). our serological results showed a high level of positivity for bvdv-1 and bvdv-2 within the buffalo herd. the molecular ana ... | 2015 | 25962538 |
| prevalence study and genetic typing of bovine viral diarrhea virus (bvdv) in four bovine species in china. | to determine the nationwide status of persistent bvdv infection in different bovine species in china and compare different test methods, a total of 1379 serum samples from clinical healthy dairy cattle, beef cattle, yaks (bos grunniens), and water buffalo (bubalus bubalis) were collected in eight provinces of china from 2010 to 2013. the samples were analyzed using commercial antibody (ab) and antigen (ag) detection kits, and rt-pcr based on the 5'-utr and npro gene sequencing. results showed th ... | 2015 | 25849315 |
| comparison of acute infection of calves exposed to a high-virulence or low-virulence bovine viral diarrhea virus or a hobi-like virus. | to compare acute infection of cattle exposed to a high-virulence (hv) bovine viral diarrhea virus (bvdv), low-virulence (lv) bvdv, or hobi-like virus. | 2013 | 23438120 |
| origins and global context of brucella abortus in italy. | brucellosis is a common and chronic disease of cattle and other bovids that often causes reproductive disorders. natural infection in cattle is caused by brucella abortus and transmission typically occurs during abortions, calving, or nursing. brucellosis is also a major zoonotic disease due to contamination of dairy products or contact with the tissues of infected animals. brucellosis has been eradicated from most of the developed world in the last 40 years but persists in many regions-the dise ... | 2017 | 28152976 |
| a diagnostic protocol to identify water buffaloes (bubalus bubalis) vaccinated with brucella abortus strain rb51 vaccine. | the use of live vaccine strain rb51 for vaccination of domestic water buffaloes (bubalus bubalis) at risk of infection with brucella abortus is permitted notwithstanding the plans for the eradication and only under strict veterinary control. the antibodies induced by rb51 vaccination are not detectable using conventional diagnostic techniques; therefore, it is necessary to have a specific diagnostic tool able to discriminate vaccinated from unvaccinated animals. the combination of a complement f ... | 2015 | 26129660 |
| pheno- and genotyping of brucella abortus biovar 5 isolated from a water buffalo (bubalus bubalis) fetus: first case reported in the americas. | an isolate of brucella spp. from an aborted water buffalo (bubalus bubalis) fetus was characterized based on its pheno- and genotype. the phenotype was defined by carbon dioxide requirement, hydrogen sulfide production, sensitivity to thionin and basic fuchsin and agglutination with brucella a and m monospecific antisera. the genotype was based on the amplification of the following genes: bcsp31, omp2ab, and eri and the species-specific localization of the insertion sequence is711 in the brucell ... | 2014 | 25113673 |
| detection of antibodies against brucella abortus, leptospira spp., and apicomplexa protozoa in water buffaloes in the northeast of argentina. | water buffalo industry has become a profitable activity worldwide, including the northeast of argentina (nea). however, research on diseases affecting this species is scarce. the aim of the present study was to detect antibodies against brucella abortus, leptospira spp., neospora caninum, toxoplasma gondii, and sarcocystis spp. in 500 water buffalo cows from five ranches (100 animals each) in the nea. serum samples were tested for b. abortus by fluorescence polarization assay, leptospira spp. by ... | 2013 | 23765549 |
| serological and bacteriological responses of water buffalo (bubalus bubalis) vaccinated with two doses of brucella abortus strain rb51 vaccine. | thirty-two water buffalo (bubalus bubalis) calves aged 6–10 months were used to evaluate serological responses to brucella abortus strain rb51 (rb51) vaccination in a dose-response study and to compare the use of two selective media for the isolation of rb51. the animals were randomly divided into three treatment groups. groups i-iii received the recommended vaccine dose (rd) twice 4 weeks apart, rd twice 18 weeks apart and saline once, respectively. lymph nodes were excised from the three group ... | 2012 | 22297422 |
| serological profile of buffalo (bubalus bubalis) female calves vaccinated with standard brucella abortus strain 19 vaccine using rose bengal, 2-mercaptoethanol and complement fixation tests. | the serological profiles of 21 female buffaloes vaccinated between 3 and 8 months of age using brucella abortus strain 19 (s19) were evaluated by rose bengal (rbt), 2-mercaptoethanol (2me) and complement fixation (cft) tests. the serum strains were collected in day zero, 15, 30, 45, 60th days and subsequently to each 30 months, until 720th day after vaccination. no animal showed reaction in day zero. in 15th day above 95% of animals revealed reaction in all tests. all the animals presented absen ... | 2012 | 22284623 |
| bubaline herpesvirus 1 associated with abortion in a mediterranean water buffalo. | during routine analysis of water buffalo foetuses, one sample was positive for herpesvirus and negative to all the other abortive agents investigated. sequencing of the herpesvirus glycoprotein e gene identified the virus as bubaline herpesvirus 1, showing few differences with the published sequences. this represents the first finding of bubaline herpesvirus in a water buffalo foetus associated with abortion. | 2013 | 23332497 |
| detection of campylobacter in human and animal field samples in cambodia. | campylobacter are zoonotic bacteria and a leading cause of human gastroenteritis worldwide with campylobacter jejuni and c. coli being the most commonly detected species. the aim of this study was to detect campylobacter in humans and livestock (chickens, ducks, pigs, cattle, water buffalo, quail, pigeons and geese) in rural households by routine culturing and multiplex pcr in faecal samples frozen before analysis. of 681 human samples, 82 (12%) tested positive by pcr (c. jejuni in 66 samples an ... | 2016 | 26991032 |
| two different macaviruses, ovine herpesvirus-2 and caprine herpesvirus-2, behave differently in water buffaloes than in cattle or in their respective reservoir species. | the ongoing global spread of "exotic" farm animals, such as water buffaloes, which carry their native sets of viruses, may bear unknown risks for the animals, into whose ecological niches the former are introduced and vice versa. here, we report on the occurrence of malignant catarrhal fever (mcf) on swiss farms, where "exotic" water buffaloes were kept together with "native" animals, i.e. cattle, sheep, and goats. in the first farm with 56 water buffaloes, eight cases of mcf due to ovine herpes ... | 2013 | 24386255 |
| novel sequence types of chlamydia pecorum infect free-ranging alpine ibex (capra ibex) and red deer (cervus elaphus) in switzerland. | chlamydia pecorum, a recognized pathogen of domesticated ruminants and koalas (phascolarctos cinereus), has been recently reported in a broad range of other wildlife species including water buffalo (bubalus bubalis), ibex (capra ibex), chamois (rupicapra rupicapra), red deer (cervus elaphus), and birds. this identification raises questions as to whether cross-host transmission may be a factor in the epidemiology of infections in these species. to begin to address this question, we employed a c. ... | 2015 | 25647593 |
| chlamydiaceae in riverine buffalo (bubalus bubalis) and cows (bos taurus) in egypt with and without signs of reproductive disease. | to obtain information and compare the prevalence of chlamydiaceae in riverine buffalo (bubalus bubalis) and cows (bos taurus) in egypt with and without clinical signs of reproductive disease. | 2012 | 22712774 |
| diversity of salmonella spp. serovars isolated from the intestines of water buffalo calves with gastroenteritis. | salmonellosis in water buffalo (bubalus bubalis) calves is a widespread disease characterized by severe gastrointestinal lesions, profuse diarrhea and severe dehydration, occasionally exhibiting a systemic course. several salmonella serovars seem to be able to infect water buffalo, but salmonella isolates collected from this animal species have been poorly characterized. in the present study, the prevalence of salmonella spp. in water buffalo calves affected by lethal gastroenteritis was assesse ... | 2012 | 23098237 |
| effect on quarter milk somatic cell count and antimicrobial susceptibility of staphylococcus rostri causing intramammary infection in dairy water buffaloes. | in many parts of the world, coagulase-negative staphylococci (cns) are the predominant cause of intramammary infections (imi) in dairy cows and in water buffaloes, as well. a longitudinal field study was carried out on one well-managed dairy water buffalo herd to determine the prevalence and distribution of cns and a recently described cns-species, staphylococcus rostri, in milk samples to explore its relevance for buffaloes' udder health throughout lactation, and to gain insight into the suscep ... | 2013 | 23548306 |
| molecular detection of coxiella burnetii using an alternative loop-mediated isothermal amplification assay (lamp). | q fever, caused by coxiella burnetii, is a worldwide zoonosis with important consequences for human and animal health. in livestock, the diagnosis, using direct and indirect techniques, is challenging even if to tackle coxiellosis in domesticated animals a rapid diagnosis is crucial. in the recent years, new molecular methods have been developed to overcome these issues. several polymerase chain reaction (pcr) assays have been studied, but loop mediated isothermal amplification (lamp) has not be ... | 2015 | 25842216 |
| investigation of the prion protein gene (prnp) polymorphisms in anatolian, murrah, and crossbred water buffaloes (bubalus bubalis). | bovine spongiform encephalopathy (bse) of the cattle is the outstanding disease among other transmissible spongiform encephalopathy (tses). it can be transmitted from the cattle to a human and causes a new variant of the creutzfeldt-jakob disease (cjd). it is known that prion protein coding gene (prnp) plays a major role in the tse susceptibility or resistance in some species. recent researches demonstrated that the insertion (in) and deletion (del) polymorphisms within promoter and intron 1 reg ... | 2017 | 27822596 |
| first molecular characterisation of cryptosporidium and giardia from bubalus bubalis (water buffalo) in victoria, australia. | we conducted a molecular epidemiological survey of cryptosporidium and giardia from bubalus bubalis (water buffalo) on two extensive farms (450 km apart) in victoria, australia. faecal samples (n=476) were collected from different age groups of water buffalo at two time points (six months apart) and tested using a pcr-based mutation scanning-targeted sequencing-phylogenetic approach, employing markers within the small subunit of ribosomal rna (designated pssu) and triose phosphate isomerase (ptp ... | 2013 | 23886616 |
| identity and public health potential of cryptosporidium spp. in water buffalo calves in egypt. | little is known about the diversity and public health significance of cryptosporidium species in water buffaloes. in this study, we examined the distribution of cryptosporidium spp. in water buffalo calves in egypt. rectal fecal specimens from 179 calves and 359 adults were screened microscopically for cryptosporidium oocysts using modified ziehl-neelsen stain. cryptosporidium spp. in 17 microscopy-positive specimens from calves were genotyped by dna sequence analysis of the small-subunit rrna g ... | 2013 | 22963712 |
| yeasts and hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil. | the aim of this work was to study the yeast populations and the main hygienic-sanitary microbial indicators in water buffalo mozzarella produced and commercialized in minas gerais, brazil. forty-two water buffalo mozzarella samples were purchased from retail outlets in belo horizonte. in addition, five samples of consecutive starter cultures, curd before acidification, acidified curd and mozzarella were collected at an industry in the city of oliveira. only three of the five water samples analyz ... | 2013 | 24516436 |
| molecular characterization of a new microvariant of the g3 genotype for echinococcus granulosus in water buffalo in iran. | in this study, molecular characterization of echinococcus granulosus sample obtained from water buffalo originating from southwest of iran was performed using comparative sequence analysis of cox1 mitochondrial gene. dna was extracted from protoscoleces removed from hydatid cyst from the liver of a 2-year-old male buffalo slaughtered in khuzestan province. molecular and phylogenetic analyses were conducted based on cox1 mitochondrial gene. we found the presence of a new microvariant of g3 genoty ... | 2015 | 25992257 |
| diversity of lactic acid bacteria isolated from brazilian water buffalo mozzarella cheese. | the water buffalo mozzarella cheese is a typical italian cheese which has been introduced in the thriving brazilian market in the last 10 y, with good acceptance by its consumers. lactic acid bacteria (lab) play an important role in the technological and sensory quality of mozzarella cheese. in this study, the aim was to evaluate the diversity of the autochthones viable lab isolated from water buffalo mozzarella cheese under storage. samples were collected in 3 independent trials in a dairy indu ... | 2015 | 25597646 |
| phylogenetic group determination of escherichia coli isolated from animals samples. | this study analyzes the occurrence and distribution of phylogenetic groups of 391 strains of escherichia coli isolated from poultry, cattle, and water buffalo. the frequency of the phylogroups was a = 19%, b1 = 57%, b2 = 2.3%, c = 4.6%, d = 2.8%, e = 11%, and f = 3.3%. phylogroups a (p < 0.001) and f (p = 0.018) were associated with e. coli strains isolated from poultry, phylogroups b1 (p < 0.001) and e (p = 0.002) were associated with e. coli isolated from cattle, and phylogroups b2 (p = 0.003) ... | 2015 | 26421310 |
| development of an indirect elisa using different fragments of recombinant ncgra7 for detection of neospora caninum infection in cattle and water buffalo. | dense granules are immunodominant proteins for the standardization of immunodiagnostic procedures to detect neosporosis. in the presented study different fragment of a dense-granule protein was evaluated for serodiagnosis of neospora caninum in cattle and water buffalo. | 2017 | 25904948 |
| identification of fluoroquinolone-resistant extended-spectrum β-lactamase (ctx-m-8)-producing escherichia coli st224, st2179 and st2308 in buffalo (bubalus bubalis). | | 2014 | 24928853 |
| transcriptional profile of endometrial tlr4 and 5 genes during the estrous cycle and uterine infection in the buffalo (bubalus bubalis). | endometritis is one of the leading causes of infertility in the cattle and buffalo and innate immune mechanism plays an important role in clearing the infection. in this regard, endometrial expression and function of toll like receptors (tlr) are focus of investigation in the recent years. in this study, we report the transcriptional profiles of tlr4 and 5 in the buffalo endometrium during the follicular, early, mid and late luteal phases of estrous cycle and 'subclinical and clinical endometrit ... | 2014 | 24531997 |
| prevalence and etiology of subclinical mastitis among buffaloes (bubalus bubalus) in namakkal, india. | milk samples from 206 apparently healthy buffaloes of marginal farmers maintained under the prevailing field conditions were screened for subclinical mastitis (scm) to determine its prevalence and etiology by white side test (wst), california mastitis test (cmt), somatic cell count (scc) and bacteriological examination. 26.20% of animals were positive for scm in scc and bacteriological examination. prevalence in graded murrah buffaloes were found to be higher compared to graded surti and non-des ... | 2013 | 24506047 |
| cloning, e. coli expression, and characterization of heart lactate dehydrogenase b from river buffalo (bubalus bubalis). | lactate dehydrogenase is an enzyme of glycolytic pathway which catalyzes the interconversion of pyruvate and lactate. the present study describes cdna cloning, e. coli expression and characterization of lactate dehydrogenase b (ldh-b) from the heart ventricles of river buffalo (bubalus bubalis). total rna was isolated from the heart tissue, a 1005bp cdna encoding complete polypeptide chain of 334 amino acids was generated by reverse transcriptase reaction and analyzed for nucleotide sequence. th ... | 2014 | 24299182 |
| efficiency of ciprofloxacin for bacterial control, post-thaw quality, and in vivo fertility of buffalo spermatozoa. | ciprofloxacin (cp) was evaluated for bacterial control, post-thaw quality, and fertility of buffalo semen. pseudomonas aeruginosa, escherichia coli, proteus sp., corynebacterium sp., micrococcus sp., and staphylococcus sp. were isolated from buffalo semen. pseudomonas aeruginosa, corynebacterium sp., and micrococcus sp. were resistant to streptomycin, whereas p. aeruginosa and proteus sp. were resistant to penicillin. all bacteria were susceptible to cp. in vitro dose toxicity was assessed in so ... | 2013 | 23746693 |
| isolation of a gene encoding a cellulolytic enzyme from swamp buffalo rumen metagenomes and its cloning and expression in escherichia coli. | ruminants are capable of hydrolyzing lignocellulosic residues to absorbable sugars by virtue of the microbial communities residing in their rumen. however, large sections of such microbial communities are not yet culturable using conventional laboratory techniques. therefore in the present study, the metagenomic dna of swamp buffalo (bubalus bubalis) rumen contents was explored using culture-independent techniques. the consensus regions of glycosyl hydrolase 5 (gh5) family of cellulases were use ... | 2012 | 23134306 |
| molecular cloning, sequence characterization and heterologous expression of buffalo (bubalus bubalis) oviduct-specific glycoprotein in e. coli. | oviductin is a high molecular weight oviduct-specific glycoprotein secreted by the non-ciliated epithelial cells of oviduct during estrous cycle and early pregnancy. it plays an important role during fertilization and early embryonic development. the oviductin gene from oviductal tissues of buffalo was successfully cloned and sequenced. the sequence analysis revealed that buffalo and cattle oviductin share very high homology between their cdna sequences. the predicted amino acid sequences of the ... | 2012 | 22782592 |
| 2d-page analysis of the soluble proteins of the tropical liver fluke, fasciola gigantica and biliary amphistome, gigantocotyle explanatum, concurrently infecting bubalus bubalis. | the digenetic trematodes, fasciola gigantica and gigantocotyle explanatum, belonging to the family fasciolidae and paramphistomidae respectively, have been often found to concurrently infect the liver of indian water buffalo bubalus bubalis, causing serious pathological damage to the vital organ, incurring huge economic losses. in the present study the soluble gene products of both f. gigantica and g. explanatum were analyzed by 2 dimensional polyacrylamide gel electrophoresis. the soluble prote ... | 2016 | 27605808 |
| anthelmintic effect of biocompatible zinc oxide nanoparticles (zno nps) on gigantocotyle explanatum, a neglected parasite of indian water buffalo. | helminth parasites of veterinary importance cause huge revenue losses to agrarian economy worldwide. with the emergence of drug resistance against the current formulations, there is a need to focus on the alternative approaches in order to control this menace. in the present study, biocompatible zinc oxide nanoparticles (zno nps) were used to see their in vitro effect on the biliary amphistomes, gigantocotyle explanatum, infecting bubalus bubalis because these nanoparticles are involved in gener ... | 2015 | 26177503 |
| somatic antigens of tropical liver flukes ameliorate collagen-induced arthritis in wistar rats. | parasitic helminths polarize immune response of their vertebrate hosts towards anti-inflammatory th2 type and therefore it is hypothesized that they may suppress the inflammatory conditions in autoimmune disorders. the present study was undertaken to investigate in vivo immunomodulatory and therapeutic potential of somatic antigens (ag) of liver infecting digenetic trematodes [fasciola gigantica (fg) and gigantocotyle explanatum (ge)] in collagen-induced arthritic (cia) wistar rats. the cia rats ... | 2015 | 25992888 |
| immunodetection of coproantigens for the diagnosis of amphistomosis in naturally infected indian water buffalo, bubalus bubalis. | the infection of gastrointestinal helminths in livestock is routinely diagnosed by microscopical examination of faecal samples for the presence of ova/eggs but this approach becomes ineffective for the seasonally egg producing trematodes. therefore, an alternative approach to detect the coproantigens of liver and rumen amphistomes, gigantocotyle explanatum and gastrothylax crumenifer respectively, infecting indian water buffalo bubalus bubalis, was undertaken using elisa, immunodot and countercu ... | 2013 | 23036808 |
| characterization of the glutamate dehydrogenase activity of gigantocotyle explanatum and gastrothylax crumenifer (trematoda: digenea). | glutamate dehydrogenase (gldh) (ec 1.4.1.3) is a ubiquitous enzyme, which is present at the protein and carbohydrate metabolism crossroads. the enzyme activity was investigated in biliary and rumen amphistomes, gigantocotyle explanatum and gastrothylax crumenifer, respectively, infecting the indian water buffalo bubalus bubalis. the enzyme activity was consistently higher in g. explanatum as compared to g. crumenifer, where nad(h) was utilized as coenzyme and the ph optima was recorded at 8. the ... | 2009 | 23129890 |
| transcriptomic responses of water buffalo liver to infection with the digenetic fluke fasciola gigantica. | fasciola gigantica, the tropical liver fluke, infects buffaloes in asian and african countries and causes significant economic losses and poses public health threat in these countries. however, little is known of the transcriptional response of buffaloes to infection with f. gigantica. the objective of the present study was to perform the first transcriptomic analysis of buffalo liver infected by f. gigantica. understanding the mechanisms that underpin f. gigantica infection in buffaloes will co ... | 2017 | 28143561 |
| molecular characterization and phylogenetic analysis of fasciola gigantica from western java, indonesia. | fasciola gigantica and aspermic (hybrid) fasciola flukes are thought to be distributed in southeast asian countries. the objectives of this study were to investigate the distribution of these flukes from unidentified ruminants in western java, indonesia, and to determine their distribution history into the area. sixty fasciola flukes from western java were identified as f. gigantica based on the nucleotide sequences of the nuclear phosphoenolpyruvate carboxykinase (pepck) and dna polymerase delt ... | 2016 | 27266482 |
| molecular phylogenetic analysis of fasciola flukes from eastern india. | fasciola flukes from eastern india were characterized on the basis of spermatogenesis status and nuclear its1. both fasciola gigantica and aspermic fasciola flukes were detected in imphal, kohima, and gantoku districts. the sequences of mitochondrial nad1 were analyzed to infer their phylogenetical relationship with neighboring countries. the haplotypes of aspermic fasciola flukes were identical or showed a single nucleotide substitution compared to those from populations in the neighboring coun ... | 2015 | 25936970 |
| high prevalence of schistosoma japonicum and fasciola gigantica in bovines from northern samar, the philippines. | the cause of zoonotic schistosomiasis in the philippines is schistosoma japonicum, which infects up to 46 mammalian hosts, including humans and bovines. in china, water buffaloes have been identified as major reservoir hosts for schistosomiasis japonica, contributing up to 75% of human transmission. in the philippines, water buffaloes (carabao; bubalus bubalis carabanesis) have, historically, been considered unimportant reservoirs. we therefore revisited the possible role of bovines in schistoso ... | 2015 | 25643317 |
| prevalence of infection and molecular confirmation by using its-2 region of fasciola gigantica found in domestic cattle from chiang mai province, thailand. | to investigate the infection of fasciola gigantica (f. gigantica) in domestic cattle from chiang mai province and molecular confirmation using its-2 region. | 2014 | 24507641 |
| utilization of elisa using thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of schistosoma japonicum infection among water buffaloes. | the presence of animal reservoirs in schistosoma japonicum infection has been a major obstacle in the control of schistosomiasis. previous studies have proven that the inclusion of control measures on animal reservoir hosts for schistosomiasis contributed to the decrease of human cases. animal surveillance should therefore be included to strengthen and improve the capabilities of current serological tests. | 2012 | 22953018 |
| rumen adaptation of swamp buffaloes (bubalus bubalis) by high level of urea supplementation when fed on rice straw-based diet. | four rumen-fistulated swamp buffaloes (bubalus bubalis) were randomly allocated to investigate rumen adaptation of urea on feed intake, nutrient digestibility, fermentation efficiency, and microbial protein synthesis. buffaloes were fed with rice straw ad libitum for a period of 2 weeks and then were shifted to a step-up diet regimen by supplementation of concentrate containing 20 and 40 g/kg urea at 5 g/kg bw for a period of 2 and 4 weeks, respectively. the results revealed that feed intake and ... | 2016 | 27107749 |
| use of real-time pcr technique in determination of major fibrolytic and non fibrolytic bacteria present in indian surti buffaloes (bubalus bubalis). | in the milk industry in india, buffalo breeds are most commonly used for milk production. efficiency of fiber digestion in ruminants is critical for animal productivity. bacteria play an important role in fiber digestion and utilization. absolute quantification real-time pcr was used to quantify ten bacterial species in rumen fluid of surti buffalo fed green fodder, dry roughage and compound concentrate mixture. abundance of each target taxon was calculated as a fraction of the total 16s rrna ge ... | 2013 | 24053023 |
| microbial population in the rumen of swamp buffalo (bubalus bubalis) as influenced by coconut oil and mangosteen peel supplementation. | four, rumen fistulated swamp buffalo bulls were used to study microbial populations in the rumen when supplemented with coconut oil and mangosteen peel. animals were randomly assigned to a 4 × 4 latin square design. four treatments were un-supplemented (control), supplementation with coconut oil at 50 g/kg (co5), supplementation with mangosteen peel at 30 g/kg (mp3) and supplementation with co5 and mp3 (com), of total dm intake. animals received concentrate at 10 g/kg of bw, and rice straw was g ... | 2013 | 22356106 |
| molecular investigation of foot-and-mouth disease virus in domestic bovids from gharbia, egypt. | an outbreak of foot-and-mouth disease (fmd) affecting cattle and water buffalo (bubalus bubalis) occurred in egypt during 2012/2013. the present study was undertaken to determine the current strains of the fmd virus (fmdv) and the prevalence of fmd among cattle and buffalo in gharbia, egypt. the diagnostic sensitivity of two rt-pcr assays for the detection of fmdv was evaluated. the results revealed that sat2 was the causative agent. the percentage of infected of animals varied with the detectio ... | 2014 | 25187028 |
| transmission of foot-and-mouth disease virus from experimentally infected indian buffalo (bubalus bubalis) to in-contact naïve and vaccinated indian buffalo and cattle. | this study investigated the transmission of foot-and-mouth disease virus (fmdv) from experimentally infected indian buffalo to in-contact naïve and vaccinated cattle and buffalo. in each of six rooms, two donor buffalo that had been inoculated with fmdv were housed for five days with four recipient animals, comprising one vaccinated buffalo, one vaccinated calf, one unvaccinated buffalo and one unvaccinated calf. vaccination was carried out with current indian vaccine strain (o/ind/r2/75) and ch ... | 2014 | 24837776 |
| intraspecific and interspecific genetic variation of gongylonema pulchrum and two rodent gongylonema spp. (g. aegypti and g. neoplasticum), with the proposal of g. nepalensis n. sp. for the isolate in water buffaloes from nepal. | the gullet worm (gongylonema pulchrum) has been recorded from a variety of mammals worldwide. in an earlier study, we demonstrated two separate transmission cycles in cattle (bos taurus) and wild mammals in japan based on nucleotide sequences of the ribosomal rna gene (rdna) and cytochrome c oxidase subunit i (cox-1) region of mitochondrial dna of multiple isolates of different origins. our earlier study additionally demonstrated two major cox-1 haplotypes of g. pulchrum prevalent in cattle in j ... | 2016 | 26531300 |
| a distinct genetic population of gongylonema pulchrum from water buffaloes in nepal. | whole-length esophagi of 111 murrah cross water buffaloes (bubalus bubalis) were collected in the kathmandu and chitwan districts of nepal from december 2009 to february 2010. gullet worms showing a typical epithelium-dwelling character were detected in 13 of 53 (24.5%) buffaloes in kathmandu and in 5 of 58 (8.6%) buffaloes in chitwan. the worms' morphology and measurements were identical to those of gongylonema pulchrum molin, 1857, except for the length of the left spicules relative to the bod ... | 2013 | 23421498 |
| molecular characterization of various trichomonad species isolated from humans and related mammals in indonesia. | trichomonad species inhabit a variety of vertebrate hosts; however, their potential zoonotic transmission has not been clearly addressed, especially with regard to human infection. twenty-one strains of trichomonads isolated from humans (5 isolates), pigs (6 isolates), rodents (6 isolates), a water buffalo (1 isolate), a cow (1 isolate), a goat (1 isolate), and a dog (1 isolate) were collected in indonesia and molecularly characterized. the dna sequences of the partial 18s small subunit ribosoma ... | 2014 | 25352694 |
| inhibitory potential of buffalo (bubalus bubalis) colostrum immunoglobulin g on klebsiella pneumoniae. | the unique components of colostrum like free oligosaccharides and glycoconjugates are known to offer resistance to enzymatic digestion in the gastrointestinal tract and have the ability to inhibit the localized adherence of enteropathogens to the digestive tract of the neonates. in this context, we have evaluated the in vitro effect of buffalo colostrum immunoglobulin g on human pathogen klebsiella pneumoniae, a predominant multidrug resistant pathogen associated with nasocomial infections. the ... | 2016 | 27017977 |
| probiotic properties of lactic acid bacteria isolated from water-buffalo mozzarella cheese. | this study evaluated the probiotic properties (stability at different ph values and bile salt concentration, auto-aggregation and co-aggregation, survival in the presence of antibiotics and commercial drugs, study of β-galactosidase production, evaluation of the presence of genes encoding mapa and mub adhesion proteins and ef-tu elongation factor, and the presence of genes encoding virulence factor) of four lab strains (lactobacillus casei sjrp35, leuconostoc citreum sjrp44, lactobacillus delbru ... | 2014 | 25117002 |
| animal reservoirs of visceral leishmaniasis in india. | visceral leishmaniasis (vl) is a disease that has both zoonotic and anthroponotic etiologies. in india, vl is endemic, considered to be anthroponotic, and caused by leishmania donovani . anthroponotic diseases are maintained by transmission from human to human and to a lesser extent from human to animals. serum samples from 1,220 animals from 7 human vl endemic districts of bihar, india, were tested for antibodies to a recombinant kinetoplast antigen (rk39 antigen) present in amastigotes of visc ... | 2013 | 22765517 |
| leuconostoc mesenteroides sjrp55: a bacteriocinogenic strain isolated from brazilian water buffalo mozzarella cheese. | the production of bacteriocins by leuconostoc mesenteroides represents an important opportunity for exploration of their potential use for industrial purpose. the antimicrobial compounds produced by l. mesenteroides subsp. mesenteroides sjrp55 strain were characterized and purified. cell-free supernatant of leuc. mesenteroides subsp. mesenteroides sjrp55 produced antibacterial compounds against listeria spp. strains and not inhibiting against lactobacillus spp. the antimicrobial substances were ... | 2014 | 24907159 |
| influence of cutting and deboning operations on the microbiological quality and shelf life of buffalo meat. | considering the specific biochemical composition of buffalo (bubalus bubalis) meat (high iron content, high biological value proteins and essential fatty acids, low amounts of fat and cholesterol), we evaluated the influence of cutting and deboning operations on the microbiological quality and shelf-life of vacuum-packed buffalo meat stored under refrigeration. on the processing day, samples were collected from carcass, deboning room surfaces and meat cuts. samples from meat cuts were evaluated ... | 2016 | 26897086 |
| g-protein-coupled chemokine receptor gene in lumpy skin disease virus isolates from cattle and water buffalo (bubalus bubalis) in egypt. | lumpy skin disease virus (lsdv), sheep poxvirus (spv) and goat poxvirus (gpv) are the most serious poxviruses of ruminants. in this study, we analysed the g-protein-coupled chemokine receptor (gpcr) genes of lsdv isolates from cattle and water buffalo (bubalus bubalis) in egypt during the summer of 2011. multiple alignments of the nucleotide sequences revealed that the water buffalo lsdv isolate differed from the cattle isolate at four nucleotide positions, and both isolates had nine nucleotide ... | 2016 | 25754131 |
| pharmacokinetics of amoxicillin trihydrate in thai swamp buffaloes (bubalus bubalis): a pilot study. | this study aimed to investigate the pharmacokinetic characteristics of amoxicillin (amx) in thai swamp buffaloes, bubalus bubalis, following single intramuscular administration at two dosages of 10 and 20 mg/kg body weight (b.w.). blood samples were collected at assigned times up to 48 h. the plasma concentrations of amx were measured by liquid chromatography-tandem mass spectrometry (lc-ms/ms). the concentrations of amx in the plasma were determined up to 24 h after i.m. administration at both ... | 2017 | 27292774 |
| comparison of pharmacokinetics of marbofloxacin after subcutaneous administration of various multiple-dose regimens to water buffalo calves (bubalus bubalis). | to determine pharmacokinetics of marbofloxacin in water buffalo calves (bubalus bubalis) after multiple sc administrations and to assess differences in regimen efficacy. | 2014 | 25419804 |
| clinical and pathological insights into johne's disease in buffaloes. | alternative diagnostic tools and interesting epidemiological assumptions were associated with an outbreak of johne's disease. in a buffalo herd infected with paratuberculosis, seven clinically affected animals and 21 animals with anti-mycobacterium avium elisa reactions were identified. total herd included 203 buffaloes. most lesions were comparable to those described in buffaloes and cattle affected by johne's disease. water buffalo behaviors such as communal nursing and allosuckling may be add ... | 2012 | 22528538 |
| paratuberculosis in buffaloes in northeast brazil. | several farms in the northeast of brazil were investigated for mycobacterium avium subsp. paratuberculosis infection in order to identify the occurrence of paratuberculosis in buffaloes. samples were obtained from 17 farms, two slaughter houses, and a quarantine area in the northeast. about 15,000 buffaloes of the murrah, mediterranean, and jafarabadi breed as well as their crossbreeds were evaluated for meat, dairy, and mixed farms with semi-intensive or extensive breeding practices. for diagno ... | 2016 | 27334632 |
| comparison of the tuberculin test, histopathological examination, and bacterial culture for the diagnosis of tuberculosis (mycobacterium bovis) in buffaloes (bubalus bubalis) in brazil. | tuberculosis is a disease with a great zoonotic potential. it is considered a major obstacle to cattle production and is responsible for severe losses in several production systems. a comparative cervical test (cct) was performed in 1140 buffaloes from different mesoregions of the state of pará, brazil, with the aim of comparing the sensitivity and specificity of cct with histopathological examination and bacterial culture. of the animals tested using cct, 4.65% (53/1140) were positive, 2.98% (3 ... | 2015 | 25958174 |
| identification of single nucleotide polymorphisms in toll-like receptor candidate genes associated with tuberculosis infection in water buffalo (bubalus bubalis). | toll-like receptors play a key role in innate immunity by recognizing pathogens and activating appropriate responses. pathogens express several signal molecules (pathogen-associated molecular patterns, pamps) essential for survival and pathogenicity. recognition of pamps triggers an array of anti-microbial immune responses through the induction of various inflammatory cytokines. the objective of this work was to perform a case-control study to characterize the distribution of polymorphisms in th ... | 2014 | 25496717 |
| molecular detection of mycobacterium tuberculosis in cattle and buffaloes: a cause for public health concern. | tuberculosis is a re-emerging disease causing a growing public health burden. the current study was conducted to investigate the occurrence of mycobacterium tuberculosis among cattle and buffaloes with tuberculous lesions. typical tuberculous lesions were collected from 34 cattle and 34 buffaloes (bubalus bubalis) through postmortem examination of slaughtered animals in abattoirs. dnas were extracted from samples, and m. tuberculosis was identified by pcr. positive samples were examined for resi ... | 2016 | 27517579 |
| a review of neospora caninum in water buffalo (bubalus bubalis). | a number of countries in the world have reported infections with neospora caninum in water buffalo (bubalus bubalis), from africa to asia, europe and south america and recently australia. in general, clinical manifestations (such as abortion) seem rare, which has raised the prospect that buffalo may be inherently resistant to clinical effects of n. caninum infection. worldwide, the seroprevalence of n. caninum infection (as a measure of exposure determined by the detection of antibody) in buffal ... | 2015 | 26298507 |