| dispelling the "nocardia amarae" myth: a phylogenetic and phenotypic study of mycolic acid-containing actinomycetes isolated from activated sludge foam. | right-angle branched filaments and rods micromanipulated from activated sludge foam and mixed liquor were identified as putatively novel members of the genera gordonia, mycobacterium and rhodococcus using a combination of chemical, molecular and morphological data. pyrolysis mass spectrometric analyses of gordoniae isolated in both the present and a previous study revealed pyro-groups, distinct from validly described gordonia species, which could be equated with those based on morphological prop ... | 2002 | 12216692 |
| role of surgery in rhodococcus equi pulmonary infections. | rhodococcus equi (r. equi) is a soil-dwelling bacterium that is increasingly associated with pulmonary infections in immunocompromised patients. while antibiotic therapy remains the cornerstone of treatment, surgery combined with antibiotics has an important role in select cases. we report two cases of pulmonary r. equi in immunocompromised patients who were treated with antibiotics, followed by surgical resection of the infected tissues, and then long-term antibiotics. both patients had excelle ... | 2002 | 12217735 |
| a new modified ortho cleavage pathway of 3-chlorocatechol degradation by rhodococcus opacus 1cp: genetic and biochemical evidence. | the 4-chloro- and 2,4-dichlorophenol-degrading strain rhodococcus opacus 1cp has previously been shown to acquire, during prolonged adaptation, the ability to mineralize 2-chlorophenol. in addition, homogeneous chlorocatechol 1,2-dioxygenase from 2-chlorophenol-grown biomass has shown relatively high activity towards 3-chlorocatechol. based on sequences of the n terminus and tryptic peptides of this enzyme, degenerate pcr primers were now designed and used for cloning of the respective gene from ... | 2002 | 12218013 |
| characterization of virulence plasmid types in rhodococcus equi isolates from foals, pigs, humans and soil in hungary. | rhodococcus equi isolates (204) obtained from foals (lung abscesses, lymph nodes, nasal discharge, rectal swabs) bred in 15 studs located throughout hungary, isolates from soil samples, lymph nodes of pigs and from lesions of human patients were examined to determine genotypic diversity of virulence-associated plasmids. isolates were tested for the presence of 15-17 kda virulence-associated protein antigen (vapa) and 20k da (vapb) genes by polymerase chain reaction (pcr). plasmid dnas were isola ... | 2002 | 12220812 |
| isolation and characterization of dibenzofuran-degrading actinomycetes: analysis of multiple extradiol dioxygenase genes in dibenzofuran-degrading rhodococcus species. | sixteen actinomycetes capable of utilizing dibenzofuran as a sole source of carbon and energy were isolated, including rhodococcus, microbacterium, and terrabacter genera. heretofore, no dibenzofuran-utilizing strain belonging to the genus microbacterium has been reported. five extradiol dioxygenase genes (dfdb, and edil to 4) of the strain rhodococcus sp. yk2 were cloned and analyzed. the nucleotide sequence of dfdb gene was almost identical to the bphc1 gene of terrabacter sp. dpo360, which wa ... | 2002 | 12224629 |
| flavin reductase coupling with two monooxygenases involved in dibenzothiophene desulfurization: purification and characterization from a non-desulfurizing bacterium, paenibacillus polymyxa a-1. | the dibenzothiophene (dbt) desulfurizing bacterium metabolizes dbt to form 2-hydroxybiphenyl without breaking the carbon skeleton. of the dbt desulfurization enzymes, dszc and dsza catalyze monooxygenation reactions, both requiring flavin reductase. we searched for non-dbt-desulfurizing microorganisms producing a flavin reductase that couples more efficiently with dszc than that produced by the dbt desulfurizing bacterium rhodococcus erythropolis d-1, and found paenibacillus polymyxa a-1 to be a ... | 2002 | 12226720 |
| isolation and characterization of a moderate thermophile, mycobacterium phlei gtis10, capable of dibenzothiophene desulfurization. | an organism, identified as mycobacterium phlei gtis10, was isolated based on its ability to use dibenzothiophene (dbt) as a sole source of sulfur for growth at 30-52 degrees c. similar to other biodesulfurization-competent organisms, m. phlei gtis10 converts dbt to 2-hydroxybiphenyl (2-hbp), as detected by hplc. the specific desulfurization activity of the 50 degrees c m. phlei gtis10 culture was determined to be 1.1+/-0.07 micromol 2-hbp min(-1) (g dry cell)(-1). m. phlei gtis10 can also utiliz ... | 2002 | 12226734 |
| application of multi-parameter flow cytometry using fluorescent probes to study substrate toxicity in the indene bioconversion. | the bioconversion of indene to cis-(1s,2r) indandiol, a potential key intermediate in the synthesis of merck's hiv protease inhibitor, crixivan trade mark, can be achieved using a rhodococcus strain. this study using rhodococcus i24 reports on the application of multiparameter flow cytometry for the measurement of cell physiological properties based on cytoplasmic membrane (cm) integrity and membrane depolarization as indicators of toxic effects of the substrate, indene. quantification of intact ... | 2002 | 12226855 |
| identification of the source of faecal pollution in contaminated rivers. | identifying the source of faecal pollution is important to enable appropriate management of faecal pollution of water. four independent assays for faecal source discrimination have been developed and implemented in our lab. these assays detect fluorescent whitening agents, faecal sterols, bifidobacterium adolescentis and rhodococcus coprophilus. the combination of these indicators is able to identify human derived faecal pollution in rivers containing inputs from septic tanks, municipal oxidatio ... | 2002 | 12227609 |
| central venous catheter-related bacteremia due to tsukamurella species in the immunocompromised host: a case series and review of the literature. | we report 6 cases of bacteremia due to tsukamurella species, all of which were in immunosuppressed patients with indwelling central venous catheters (cvcs). fewer than 20 cases of serious illness due to these gram-positive bacilli have been reported in the medical literature; these cases have mostly been ascribed to the species tsukamurella paurometabola. tsukamurella species are frequently misidentified as rhodococcus or corynebacterium species. we used high-performance liquid chromatography to ... | 2002 | 12228839 |
| [primary pyomiositis by rhodococcus spp. in an immunocompetent patient]. | | 2002 | 12236992 |
| molecular epidemiology of vapa-positive rhodococcus equi in thoroughbred horses in kagoshima, japan. | the prevalence of virulent r. equi having 15- to 17-kda antigens (vapa) in fecal isolates from 13 thoroughbred foals and their dams on 5 farms in kagoshima, japan, and the plasmid profiles of vapa-positive isolates by restriction fragment digestion patterns were investigated to compare the genotypic variation among virulence plasmids of r. equi isolates from japan. in total, 218 (24.6%) of 886 isolates from the feces of the 13 foals and 13 (12.5%) of 104 isolates from the feces of their dams dem ... | 2002 | 12237518 |
| rhodococcus equi pleuropneumonia in an adult horse. | a 10-year-old warmblood gelding was evaluated for intermittent pyrexia, dullness, weight loss, and progressive respiratory disease. multifocal necrotic pneumonia and pleuritis due to rhodococcus equi infection was diagnosed. case management is discussed, as well as factors that may have led to this rare cause of pleuropneumonia in an adult horse. | 2002 | 12240529 |
| bacterial flora in foals with upper respiratory tract infections in poland. | bacteria isolated from nasal cavity of 80 foals with upper respiratory tract infection, as well as from 20 healthy foals, were examined. within the group of sick animals, from 18 (22.5%) bacteria with recognized pathogenicity were isolated. coagulase-negative staphylococci and acinetobacter sp. were the dominant species identified (100 and 45%, respectively). no bacteria species with recognized pathogenicity were isolated from the group of healthy animals. three cases of death within the group o ... | 2002 | 12241031 |
| cholesterol oxidase and resistance of rhodococcus equi to peroxidative stress in vitro in the presence of cholesterol. | rhodococcus equi is a well-characterized bacterial pathogen which lyses cell membranes with the help of cholesterol oxidase (co). survival in macrophages is warranted by its ability to resist reactive radicals via catalase and superoxide dismutase (sod). therefore, co production in the absence or presence of 0.1 % cholesterol and sensitivity to exogenous hydrogen peroxide (h2o2) and superoxide anion (soa) were tested in seven strains of r. equi in vitro. when r. equi strains were grown on agar p ... | 2002 | 12241035 |
| evaluation of 5 serologic assays to detect rhodococcus equi pneumonia in foals. | to determine the sensitivity and specificity of 5 serologic assays used to diagnose rhodococcus equi pneumonia in foals and to determine whether any of the assays could be used to identify affected foals prior to the onset of clinical signs or to differentiate between affected and unaffected foals when clinical signs first become apparent. | 2002 | 12322921 |
| cloning, sequencing, and characterization of the hexahydro-1,3,5-trinitro-1,3,5-triazine degradation gene cluster from rhodococcus rhodochrous. | hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) is a high explosive which presents an environmental hazard as a major land and groundwater contaminant. rhodococcus rhodochrous strain 11y was isolated from explosive contaminated land and is capable of degrading rdx when provided as the sole source of nitrogen for growth. products of rdx degradation in resting-cell incubations were analyzed and found to include nitrite, formaldehyde, and formate. no ammonium was excreted into the medium, and no dead ... | 2002 | 12324318 |
| molecular and functional characterization of the kstd2 gene of rhodococcus erythropolis sq1 encoding a second 3-ketosteroid delta(1)-dehydrogenase isoenzyme. | previously, rhodococcus erythropolis sq1 kstd, encoding ketosteroid delta(1)-dehydrogenase (kstd1) was characterized. surprisingly, a kstd gene deletion mutant (strain rg1) grew normally on steroids. uv mutagenesis of strain rg1 allowed isolation of strains (e.g. strain rg1-uv29) unable to perform the delta(1)-dehydrogenation of 4-androstene-3,17-dione (ad) and 9alpha-hydroxy-4-androstene-3,17-dione (9ohad). functional complementation of strain rg1-uv29 with total genomic dna of strain rg1 resul ... | 2002 | 12368462 |
| biochemical characterization and structural analysis of a highly proficient cocaine esterase. | the bacterial cocaine esterase, coce, hydrolyzes cocaine faster than any other reported cocaine esterase. hydrolysis of the cocaine benzoyl ester follows michaelis-menten kinetics with k(cat) = 7.8 s(-1) and k(m) = 640 nm. a similar rate is observed for hydrolysis of cocaethylene, a more potent cocaine metabolite that has been observed in patients who concurrently abuse cocaine and alcohol. the high catalytic proficiency, lack of observable product inhibition, and ability to hydrolyze both cocai ... | 2002 | 12369817 |
| the cbs mutant strain of rhodococcus erythropolis ka2-5-1 expresses high levels of dsz enzymes in the presence of sulfate. | two mutants of the dibenzothiophene-desulfurizing rhodococcus erythropolis ka2-5-1, strains ms51 and ms316, which express a high level of desulfurizing activity in the presence of sulfate, were isolated using the transposome technique. the level of dibenzothiophene-desulfurization by cell-free extracts prepared from mutants ms51 and ms316 grown on sulfate was about five-fold higher than that by cell-free extracts of the wild-type. this result was consistent with results of western-blot analysis ... | 2002 | 12375103 |
| selective transport and accumulation of alkanes by rhodococcus erythropolis s+14he. | selective transport and accumulation of n-alkanes by rhodococcus erythropolis s+14he was studied by growing cells on n-hexadecane, n-octadecane or the branched alkane pristane, and on mixtures of hydrocarbons. ultrastructural analysis by transmission electron microscopy (tem) revealed hydrocarbon inclusion bodies present in cells grown on the three alkanes, but not in cells grown on soluble media or exposed to nonmetabolized 2,2,4,4,6,8,8-heptamethylnonane (hmn). n-hexadecane had the highest rat ... | 2002 | 12378606 |
| detection of antibodies against rhodococcus equi in alpaca (lama pacos) in italy. | after isolating the two virulent strains of rhodococcus equi from alpaca, a serological survey of rhodococcus equi infection was carried out on 57 blood samples of alpaca collected in central italy. the survey was performed with an elisa test using a reference r. equi strain as antigen (atcc 33701). four (7.0%) sera (od greater or equal to 0.3) tested positive, while five (8.77%) were considered doubtful (od between 0.2 and 0.3). this is the first serological survey of r. equi infection in alpac ... | 2001 | 12380719 |
| purification and characterization of the monooxygenase catalyzing sulfur-atom specific oxidation of dibenzothiophene and benzothiophene from the thermophilic bacterium paenibacillus sp. strain a11-2. | a benzothiophene (bt) and dibenzothiophene (dbt) monooxygenase (tdsc), which catalyzes the oxidation of the sulfur atoms in bt and dbt molecules, was purified from paenibacillus sp. strain a11-2. the molecular mass of the purified enzyme and its subunit were determined to be 200 kda and 43 kda by gel filtration and sodium dodecyl sulfate polyacrylamide gel electrophoresis, respectively, indicating a tetrameric structure. the n-terminal amino acid sequence of the purified tdsc completely matched ... | 2002 | 12382053 |
| [effect of shale kerogen oxidation products on biodegradation of petroleum and petroleum products in soil and water]. | effect of oxidation products of shale kerogen (high-molecular-weight acids with 6-22 carbon atoms) on biodegradation of oil and oil products in soil and water was studied. high-molecular-weight acids (hmwa) not only affected the layer of oil and/or oil products and dispersed it into small particles, but also stimulated growth of rhodococcus erythropolis vkm as-1339d, degraders of oil and oil products. addition of 0.001-0.003% hmwa to a medium to be purified from oil products increased the extent ... | 2002 | 12391752 |
| [biosensor of the reactor type based on rhodococcus erythropolis hl tm-1 cells for determining 2,4-dinitrophenol]. | a model of a reactor-type biosensor based on the rhodococcus erythropolis hl pm-1 was developed for amperometric detection of 2,4-dinitrophenol (2,4-dnp). the effects of the matrix material (agar and calcium alginate gels, ceramic support, and cellulose powder) on the biosensor signal concentration dependence, detection time, and biosensor stability were studied. in case of bacterial cells immobilized on cellulose powder, the lower limit of 2,4-dnp detection was 20 microm and the time of single ... | 2002 | 12391763 |
| sulfur-selective desulfurization of dibenzothiophene and diesel oil by newly isolated rhodococcus sp. strains. | new desulfurizing bacteria able to convert dibenzothiophene into 2-hydroxybiphenyl and sulfate were isolated from contaminated soils collected in mexican refineries. random amplified polymorphic dna analysis showed they were different from previously reported rhodococcus erythropolis desulfurizing strains. according to 16s rrna gene sequencing and fatty acid analyses, these new isolates belonged to the genus rhodococcus. these strains could desulfurize 4,6-dimethyldibenzothiophene which is one o ... | 2002 | 12393216 |
| cerebral infection with rhodococcus equi in a heart transplant recipient. | iatrogenic immunosuppression following organ transplantation leads to an increased number of opportunistic bacterial and viral infections. similar to patients with human immunodeficiency virus (hiv) infection transplant recipients may suffer from rare infectious diseases. herein we report on a patient presenting with a cerebral infection who had previously undergone orthotopic cardiac transplantation. rhodococcus equi was isolated. | 2002 | 12398884 |
| gas exchange during intense exercise in standardbreds with earlier rhodococcus equi pneumonia. | it is not known if pulmonary function and gas exchange during exercise are altered after pyogranulomatous pneumonia caused by rhodococcus equi infection in the foal. the aim was to evaluate whether pulmonary gas exchange during high intensity exercise was altered in mature standardbreds with a history of r. equi pneumonia as foals. in 7 foals, r. equi pneumonia was confirmed and treated. at age 3 years, when these horses were subjected to professional training, an inclined treadmill exercise tes ... | 2002 | 12405730 |
| pac1 and pacap expression, signaling, and effect on the growth of hct8, human colonic tumor cells. | the pituitary adenylate cyclase-activating polypeptide (pacap) type 1 receptor (pac1) is a heptahelical, g protein-coupled receptor that has been shown to be expressed by non-squamous lung cancer and breast cancer cell lines, and to be coupled to the growth of these tumors. we have previously shown that pacap and its receptor, pac1, are expressed in rat colonic tissue. in this study, we used polyclonal antibodies directed against the cooh terminal of pac1, as well as fluorescently labeled pacap, ... | 2002 | 12409223 |
| enantioselective stereoinversion in the kinetic resolution of rac-sec-alkyl sulfate esters by hydrolysis with an alkylsulfatase from rhodococcus ruber dsm 44541 furnishes homochiral products. | | 2002 | 12412078 |
| identification of pulmonary t-lymphocyte and serum antibody isotype responses associated with protection against rhodococcus equi. | rhodococcus equi infects and causes pneumonia in foals between 2 and 4 months of age but does not induce disease in immunocompetent adults, which are immune and remain clinically normal upon challenge. understanding the protective response against r. equi in adult horses is important in the development of vaccine strategies, since those mechanisms likely reflect the protective phenotype that an effective vaccine would generate in the foal. twelve adult horses were challenged with virulent r. equ ... | 2002 | 12414760 |
| versatile persistence pathways for pathogens of animals and plants. | the glyoxylate cycle and the glycine cleavage system are part of conserved metabolic pathways involved in the chronic persistence of microorganisms in animal hosts. in the chromosome of the plant pathogen rhodococcus fascians, the vic locus has been identified as a region containing genes essential for persistence inside induced leafy galls. sequence analysis showed that this 18-kb locus is syntenic with chromosomal regions of mycobacterium species that encompass the 'persistence' loci of these ... | 2002 | 12419605 |
| a novel class of self-sufficient cytochrome p450 monooxygenases in prokaryotes. | the bacillus cytochrome p450 bm3 integrates an entire p450 system in one polypeptide and represents a convenient prokaryotic model for microsomal p450s. this self-sufficient class ii p450 is also present in actinomycetes and fungi. by genome analysis we have identified additional homologues in the pathogenic species bacillus anthracis and bacillus cereus, and in ralstonia metallidurans. this analysis also revealed a novel class of putative self-sufficient p450s, p450 pfor, comprising a class i p ... | 2002 | 12419614 |
| disposition of oral clarithromycin in foals. | clarithromycin offers numerous advantages over erythromycin and thus, is an attractive alternative for the treatment of rhodococcus equi infections in foals. the disposition of clarithromycin was investigated in 6 foals after intragastric administration at a dose of 10 mg/kg body weight. detectable serum concentrations of clarithromycin were found in 3 of 6 foals at 10 minutes and in all foals by 20 minutes post-administration. time to peak serum concentration (tmax) was 1.5 hours and peak serum ... | 2002 | 12423226 |
| isolation and characterization of bacteria from crude petroleum oil contaminated soil and their potential to degrade diesel fuel. | potential hydrocarbon degrader bacteria were isolated from soil samples that have been exposed to crude petroleum oil spills. bacterial population of these polluted soils showed counts ranging between 9.5 x 10(5) and 237.5 x 10(5) cfu/g soil with 2 different colony types of bacterial strains which have been recovered on the agar plates. results indicated that longer aged contamination exhibited a greater number of microorganisms. phenotypic examination of the recovered bacteria revealed that the ... | 2002 | 12442304 |
| serum and mucosal antibodies of infected foals recognized two distinct epitopes of vapa of rhodococcus equi. | virulence-associated protein a (vapa) of rhodococcus equi has been proposed for use both as a vaccine and as a target for antibodies in immunotherapy and diagnostic tests. epitope mapping of vapa allowed the identification of two b cell epitopes associated with r. equi pneumonia. the peptide nlqkdepgrasdt was confirmed as an immunodominant n-terminal b cell epitope recognized by all sera from infected foals while vsfqynavgpylninffdss (c-terminal b cell epitope) was exclusively recognized by iga ... | 2002 | 12443830 |
| peritoneal fluid from a febrile foal. | | 2002 | 12447781 |
| survival of foals with experimentally induced rhodococcus equi infection given either hyperimmune plasma containing r. equi antibody or normal equine plasma. | the purpose of this study was to determine if colostrum-deprived foals with experimentally induced rhodococcus equi pneumonia have a decreased severity of the disease and decreased mortality rate when given hyperimmune (hi) r. equi antibody plasma (r. equi titer at least 100 % and virulence-associated protein a [vapa] at least 10000) prophylactically versus when given normal equine plasma (r. equi titer less than 20 % and vapa less than 160). sixteen colostrum-deprived foals (r. equi titer less ... | 2002 | 12447842 |
| gene cloning and characterization of multiple alkane hydroxylase systems in rhodococcus strains q15 and nrrl b-16531. | the alkane hydroxylase systems of two rhodococcus strains (nrrl b-16531 and q15, isolated from different geographical locations) were characterized. both organisms contained at least four alkane monooxygenase gene homologs (alkb1, alkb2, alkb3, and alkb4). in both strains, the alkb1 and alkb2 homologs were part of alk gene clusters, each encoding two rubredoxins (ruba1 and ruba2; ruba3 and ruba4), a putative tetr transcriptional regulatory protein (alku1; alku2), and, in the alkb1 cluster, a rub ... | 2002 | 12450813 |
| benzothiazole degradation by rhodococcus pyridinovorans strain pa: evidence of a catechol 1,2-dioxygenase activity. | the pathway for biodegradation of benzothiazole (bt) and 2-hydroxybenzothiazole (obt) by rhodococcus pyridinovorans strain pa was studied in detail. the kinetics of biodegradation were monitored by in situ (1)h nuclear magnetic resonance (nmr) in parallel with reversed-phase high-performance liquid chromatography (hplc). successive oxidations from bt to obt and then from obt to dihydroxybenzothiazole were observed. further insight was obtained by using a mutant strain with impaired ability to gr ... | 2002 | 12450835 |
| cell-surface hydrophobicity and scum formation of rhodococcus rhodochrous strains with different colonial morphologies. | rhodococcus rhodochrous has been reported to be one of the micro-organisms responsible for the formation of scum which is thick and viscous biological foam in activated sludge plants. the hydrophobicity of mycolic acids present on the cell surface and the long-branched shape of the hyphae have been thought to contribute to the scum formation. cell surface hydrophobicity and scum formation of four r. rhodochrous strains with different colony morphologies were determined, and the results showed th ... | 1997 | 12452595 |
| microbial surfactants and their use in field studies of soil remediation. | | 2002 | 12452947 |
| crystallization and preliminary x-ray diffraction analysis of naphthalene dioxygenase from rhodococcus sp. strain ncimb 12038. | the three-component naphthalene dioxygenase (ndo) enzyme system carries out the first step in the aerobic degradation of naphthalene to (+)-cis-(1r,2s)-dihydroxy-1,2-dihydronaphthalene by rhodococcus sp. strain ncimb 12038. the terminal oxygenase component (naphthalene 1,2-dioxygenase) that catalyzes this reaction belongs to the aromatic ring hydroxylating dioxygenase family and has been crystallized. these enzymes utilize a mononuclear non-heme iron centre to catalyze the addition of dioxygen t ... | 2002 | 12454491 |
| serum amyloid a (saa) as an aid in the management of infectious disease in the foal: comparison with total leucocyte count, neutrophil count and fibrinogen. | differentiation between infectious and noninfectious disease and rapid initiation of accurate treatment are essential in managing diseases in the neonatal and young foal. identification of useful inflammatory markers for these purposes is, therefore, of great importance. the aim of this study was to compare the responses of the acute phase protein serum amyloid a (saa) with the responses of fibrinogen and total leucocyte and neutrophil counts in infectious diseases encountered in the young foal, ... | 2002 | 12455840 |
| kinetic studies of biodegradation of insoluble compounds by continuous determination of oxygen consumption. | continuous determination of oxygen consumption by electrolytic respirometry has been experimented as a means to study the biodegradation kinetics of scarcely soluble environmental pollutants. the substrates used were the polycyclic aromatic hydrocarbons (pah), naphthalene, phenanthrene, anthracene, fluoranthene and pyrene. the definition of an appropriate mode of pah supply, either as crystals or more generally as a solution in a water non-miscible solvent, was found essential for yielding repro ... | 1997 | 12455894 |
| antimicrobial activity of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), compared to other quinolones, against clinical isolates from cancer patients. | the in vitro spectrum of a novel des-fluoro (6) quinolone, garenoxacin (bms-284756), was compared with that of ciprofloxacin, levofloxacin, and trovafloxacin against 736 clinical isolates from cancer patients. garenoxacin was the most active agent overall against gram-positive organisms, with potent activity against aerococcus spp., micrococcus spp., rhodococcus equi, stomatococcus mucilaginous, bacillus spp., enterococcus faecalis, listeria monocytogenes, methicillin-susceptible staphylococcus ... | 2002 | 12458127 |
| application of broad-range 16s rrna pcr amplification and dgge fingerprinting for detection of tick-infecting bacteria. | ticks play an important role in the transmission of arthropod-borne diseases of viral, protozoal and bacterial origin. the present article describes a molecular-biological based method, which facilitated the broad-range analyses of bacterial communities in ixodid ticks (ixodes ricinus). dna was extracted both from single ticks and pooled adult ticks. eubacterial 16s rrna gene fragments (16s rdna) were amplified by polymerase chain reaction (pcr) with broad-range ribosomal primers. sequences span ... | 2003 | 12459246 |
| alkane hydroxylase homologues in gram-positive strains. | we isolated gram-positive alkane-degraders from soil and a tricking-bed reactor, and show using polymerase chain reaction (pcr) with degenerate alkane hydroxylase primers and southern blots that most rhodococcus isolates contain three to five quite divergent homologues of the pseudomonas putida gpo1 alkb gene. two mycobacterium isolates each contain one homologue, however there is no evidence for the presence of alkb homologues in the remaining strains. | 2002 | 12460275 |
| rhodococcus equi pneumonia in a mare in turkey. | | 2002 | 12463539 |
| triacylglycerols in prokaryotic microorganisms. | triacylglycerols (tag) are fatty acid triesters of glycerol; there are diverse types of tag with different properties depending on their fatty acid composition. the occurrence of tag as reserve compounds is widespread among eukaryotic organisms such as yeast, fungi, plants and animals, whereas occurrence of tag in bacteria has only rarely been described. however, accumulation of tag seems to be widespread among bacteria belonging to the actinomycetes group, such as species of mycobacterium, stre ... | 2002 | 12466875 |
| continuous desulfurization of dibenzothiophene with rhodococcusrhodochrous igts8 (atcc 53968). | desulfurization of a model fuel system consisting of hexadecane and dibenzothiophene (dbt) by rhodococcus rhodochrous igts8 was demonstrated in a 2-l continuous stirred tank reactor (cstr). the reactor was operated in a semicontinuous and continuous mode with and without recycling of the model fuel. a constant volumetric desulfurization activity a(t), (in mg hbp l(-1) h(-1)) was maintained in the reactor with a feeding strategy of fresh cell suspension based on a first-order decay of the biocata ... | 2002 | 12467453 |
| biocatalytic asymmetric hydrogen transfer. | | 2002 | 12491297 |
| methyl-beta-cyclodextrin-enhanced solubilization and aerobic biodegradation of polychlorinated biphenyls in two aged-contaminated soils. | the bioremediation of aged polychlorinated biphenyl (pcb)-contaminated soils is adversely affected by the low bioavailability of the pollutants. randomly methylated-beta-cyclodextrins (rameb) were tested as a potential pcb-bioavailability-enhancing agent in the aerobic treatment of two aged-contaminated soils. the soils, contaminated by about 890 and 8500 mg/kg of aroclor 1260 pcbs, were amended with biphenyl (4 g/kg), inorganic nutrients (to adjust their c:n ratio to 20:1), and variable amounts ... | 2003 | 12491523 |
| measurement of strain-dependent toxicity in the indene bioconversion using multiparameter flow cytometry. | the bionconversion of indene to cis-(1s,2r)-indandiol, a potential key intermediate in the synthesis of merck's hiv protease inhibitor, crixivan trade mark, can be achieved using rhodococcus, pseudomonas putida, and escherichia coli strains. this study reports on the application of multiparameter flow cytometry for the measurement of cytoplasmic membrane integrity and membrane depolarization as indicators of toxic effects of the substrate, product, and by-products using each of these strains. me ... | 2003 | 12491526 |
| rhodococcus globerulus keratitis after laser in situ keratomileusis. | a healthy 20-year-old woman with myopia had uneventful bilateral laser in situ keratomileusis after which the uncorrected visual acuity was 20/20 in the right eye and 20/30 in the left eye. fifteen days later, a stromal paraxial lesion was found in the right eye with a corresponding loss of visual acuity, pain, and photophobia. the flap was lifted and the infiltrate scraped for smears. cultures showed that rhodococcus globerulus was the infectious agent. intensive topical antibiotic treatment wa ... | 2002 | 12498868 |
| preliminary crystallographic analysis of 3-chlorocatechol 1,2-dioxygenase of a new modified ortho-pathway from the gram-positive rhodococcus opacus 1cp grown on 2-chlorophenol. | 3-chlorocatechol 1,2-dioxygenase (3-clc1,2do), a key enzyme of a new modified ortho-pathway, was isolated from a variant of the gram-positive bacterium rhodococcus opacus 1cp utilizing 2-chlorophenol as the sole energy and carbon source via a 3-chlorocatechol branch of a modified ortho-pathway. 3-clc1,2do catalyzes the intradiol cleavage of 3-chlorocatechol. the enzyme contains fe(iii) ions essential to the catalytic activity; it is a homodimer with a molecular weight of about 58 kda composed of ... | 2003 | 12499567 |
| rhodococcus maanshanensis sp. nov., a novel actinomycete from soil. | a polyphasic study was undertaken to establish the taxonomic position of a soil isolate that had provisionally been assigned to the genus rhodococcus. the organism showed a combination of phenotypic properties typical of rhodococci and formed a distinct phyletic line within the rhodococcus erythropolis 165 rdna subclade. the organism was readily distinguished from representatives of validly described species classified in this subclade on the basis of dna-dna relatedness and phenotypic data. con ... | 2002 | 12508878 |
| investigations on rhizoplane actinobacteria communities of papyrus (cyperus papyrus) from an egyptian wetland. | wetlands have important global ecological functions, which include carbon storage and water interception. wetland contributes to the maintenance of regional and global biodiversity. though many important wetland ecological functions are based on microbial metabolism, we have scanty knowledge on microbial diversity in wetlands. plant rhizoplane habitats are considered to harbor highly diverse bacterial communities. most of the floating mats on river nile are dominated by papyrus (cyperus papyrus) ... | 2002 | 12512252 |
| mrna differential display in a microbial enrichment culture: simultaneous identification of three cyclohexanone monooxygenases from three species. | mrna differential display has been used to identify cyclohexanone oxidation genes in a mixed microbial community derived from a wastewater bioreactor. thirteen dna fragments randomly amplified from the total rna of an enrichment subculture exposed to cyclohexanone corresponded to genes predicted to be involved in the degradation of cyclohexanone. nine of these dna fragments are part of genes encoding three distinct baeyer-villiger cyclohexanone monooxygenases from three different bacterial speci ... | 2003 | 12514013 |
| 2-hydroxypenta-2,4-dienoate metabolic pathway genes in a strong polychlorinated biphenyl degrader, rhodococcus sp. strain rha1. | a gram-positive polychlorinated biphenyl (pcb) degrader, rhodococcus sp. strain rha1, metabolizes biphenyl through the 2-hydroxypenta-2,4-dienoate (hpd) and benzoate metabolic pathways. the hpd metabolic pathway genes, the hpd hydratase (bphe1), 4-hydroxy-2-oxovalerate aldolase (bphf1), and acetaldehyde dehydrogenase (acylating) (bphg) genes, were cloned from rha1. the deduced amino acid sequences of bphgf1e1 have 30 to 58% identity with those of the hpd metabolic pathway genes of gram-negative ... | 2003 | 12514024 |
| photodegradation of rdx in aqueous solution: a mechanistic probe for biodegradation with rhodococcus sp. | recently we demonstrated that rhodococcus sp. strain dn22 degraded hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) (1) aerobically via initial denitration followed by ring cleavage. using ul 14c-[rdx] and ring labeled 15n-[rdx] approximately 30% of the energetic chemical mineralized (one c atom) and 64% converted to a dead end product that was tentatively identified as 4-nitro-2,4-diaza-butanal (ohchnch2nhno2). to have further insight into the role of initial denitration on rdx decomposition, we p ... | 2002 | 12523428 |
| biocatalytic asymmetric hydrogen transfer employing rhodococcus ruber dsm 44541. | nonracemic sec-alcohols of opposite absolute configuration were obtained either by asymmetric reduction of the corresponding ketone using 2-propanol as hydrogen donor or by enantioselective oxidation through kinetic resolution of the rac-alcohol using acetone as hydrogen acceptor employing whole lyophilized cells of rhodococcus ruber dsm 44541. the microbial oxidation/reduction system exhibits not only excellent stereo- and enantioselectivity but also a broad substrate spectrum. due to the excep ... | 2003 | 12530865 |
| nitrile biotransformation for highly enantioselective synthesis of 3-substituted 2,2-dimethylcyclopropanecarboxylic acids and amides. | biotransformations of differently configured 2,2-dimethyl-3-substitued-cyclopropanecarbonitriles were studied using a nitrile hydratase/amidase-containing rhodococcus sp. aj270 whole-cell catalyst under very mild conditions. although all of the cis-3-aryl-2,2-dimethylcyclopropanecarbonitriles appeared inert toward the biocatalyst, a number of racemic trans-isomers efficiently underwent a highly enantioselective hydrolysis to produce (+)-(1r,3r)-3-aryl-2,2-dimethylcyclopropanecarboxylic acids and ... | 2003 | 12530896 |
| clostridium difficile infections in animals with special reference to the horse. a review. | in human medicine, clostridium (c.) difficile is since many years a well-known cause of nosocomial diarrhea induced by antibiotic treatment. in horses, c. difficile was recently suggested as a possible enteric pathogen. the bacterium is associated with acute colitis in mature horses following treatment with antibiotics. c. difficile, and/or its cytotoxin, is also associated with acute colitis in mares when their foals are being treated with erythromycin and rifampicin for rhodococcus equi pneumo ... | 2002 | 12540137 |
| a rapid and sensitive fluorometric assay method for the determination of nitrilase activity. | a rapid, simple and sensitive fluorometric assay method for the determination of nitrilase activity is described. 3-cyanopyridine was hydrolysed to nicotinic acid by rhodococcus rhodochrous and the liberated nh(3) was allowed to react with buffered o -phthaldialdehyde-2-mercaptoethanol solution (ph 7.4) to form a fluorochrome. the fluorescence intensity was found to be stable after 20 min incubation at room temperature, and the optimum ph for the reaction was found to be 7.4. the fluorescence in ... | 2003 | 12549980 |
| [progress of biodesulfurization]. | | 2001 | 12552818 |
| cavitary rhodococcus equi pneumonia with endobronchial granulomas: report of an unusual case. | an unusual case of cavitary rhodococcus equi pneumonia with endobronchial granulomas in congenital hiv infection is presented. the clinical features and radiological manifestations of pulmonary r. equi infection are discussed. | 2003 | 12557073 |
| on the organic solvent and thermostability of the biocatalytic redox system of rhodococcus ruber dsm 44541. | the sec-alcohol dehydrogenase activity of whole cells of rhodococcus ruber dsm 44541 has been employed as an efficient biocatalytic redox system due to the use of acetone and 2-propanol at elevated concentrations for cofactor regeneration in the oxidation and reduction mode, respectively, and external addition of nadh/nad(+) can be omitted. the operational half-life time of the redox system is 29 hours in 20% v/v acetone and 37 hours in 30% v/v 2-propanol. the redox system allows the enantiosele ... | 2003 | 12557320 |
| [study on the reclassification of two deposited strains as4.1186 and as4.1187 of nocardia]. | polyphasic evidence for the reclassification of nocardia nostocoides as4.1186 and nocardia salmonicolor var. aurantiaca as4.1187 indicated that strain as4.1186 was closely related to saccharothrix texasensis and strain as4.1187 was very closely related to rhodococcus ruber. binary 16s rdna sequence for the pair as4.1186 and saccharothrix texasensis nrrl b-16134t, and the pair as4.1187 and rhodococcus ruber dsm 43338t exhibited 99.3% and 99.5% similarity, respectively. corresponding dna-dna reass ... | 2002 | 12557341 |
| pentacyclic triterpenoids from embelia schimperi. | five oleanane-type pentacyclic triterpenoids were isolated by chromatographic separation of a chloroform extract of the stem bark of embelia schimperi. three of these compounds have a methyleneoxy bridge. two compounds, embelinone and schimperinone, are reported here for the first time from a natural source (they have been synthesized previously during chemical transformations). their structures were determined by spectroscopic techniques, among which 2-d nmr was useful for complete characteriza ... | 2003 | 12560028 |
| characterization of extradiol dioxygenases from a polychlorinated biphenyl-degrading strain that possess higher specificities for chlorinated metabolites. | recent studies demonstrated that 2,3-dihydroxybiphenyl 1,2-dioxygenase from burkholderia sp. strain lb400 (dhbdlb400; ec 1.13.11.39) cleaves chlorinated 2,3-dihydroxybiphenyls (dhbs) less specifically than unchlorinated dhb and is competitively inhibited by 2',6'-dichloro-2,3-dihydroxybiphenyl (2',6'-dicl dhb). to determine whether these are general characteristics of dhbds, we characterized dhbdp6-i and dhbdp6-iii, two evolutionarily divergent isozymes from rhodococcus globerulus strain p6, ano ... | 2003 | 12562795 |
| [image of the month. pulmonary malacoplakia due to rhodococcus equi in a patient with aids]. | | 2002 | 12564097 |
| a simple method to observe organic solvent drops with a standard optical microscope. | | 2003 | 12567404 |
| desulfonation and degradation of the disulfodiphenylethercarboxylates from linear alkyldiphenyletherdisulfonate surfactants. | earlier work showed that the biodegradation of a commercial linear monoalkyldiphenyletherdisulfonate surfactant as a carbon source for microbial growth leads to the quantitative formation of corresponding disulfodiphenylether carboxylates (dsdpecs), which were not degraded. alpha-proteobacterium strain ds-1 (dsm 13023) catalyzes these reactions. these dsdpecs have now been characterized by high-pressure liquid chromatography coupled via an electrospray interface to a mass spectrometer. dsdpecs w ... | 2003 | 12571015 |
| cloning of baeyer-villiger monooxygenases from comamonas, xanthobacter and rhodococcus using polymerase chain reaction with highly degenerate primers. | to clone novel type 1 baeyer-villiger monooxygenase (bvmo) genes, we isolated or collected 25 bacterial strains able to grow on alicyclic compounds. twelve of the bacterial strains yielded polymerase chain reaction (pcr) fragments with highly degenerate primers based on the sequences of known and putative bvmos. all these fragments were found to encode peptides homologous to published bvmo sequences. the complete bvmo genes and flanking dna were cloned from a comamonas, a xanthobacter and a rhod ... | 2003 | 12588297 |
| evaluation of equine breeding farm characteristics as risk factors for development of rhodococcus equi pneumonia in foals. | to identify farm characteristics as risk factors for the development of rhodococcus equi pneumonia in foals. | 2003 | 12597420 |
| evaluation of equine breeding farm management and preventative health practices as risk factors for development of rhodococcus equi pneumonia in foals. | to determine whether foal management practices, environmental management, and preventative health practices are risk factors for development of rhodococcus equi pneumonia in foals. | 2003 | 12597421 |
| epidemiologic study of results of pulsed-field gel electrophoresis of isolates of rhodococcus equi obtained from horses and horse farms. | to compare isolates of rhodococcus equi on the basis of geographic source and virulence status by use of pulsed-field gel electrophoresis (pfge). | 2003 | 12602583 |
| a serological survey of rhodococcus equi infection in foals in central italy: comparison of two antigens using an elisa test. | a serological survey of rhodococcus equi infection was carried out on 602 blood samples collected from foals in central italy. the assay was performed with an elisa test using two different antigens prepared with reference strains of r. equi, atcc 33071 and atcc 6939. a positive reaction was obtained on 81 serum samples (13.45%) (od > or = 0.3) using antigen atcc 33071, and on 73 serum samples (12.12%) using antigen atcc 6939. although the frequency of the disease was not high, the serological p ... | 2003 | 12602683 |
| biodegradation of bis(1-chloro-2-propyl) ether via initial ether scission and subsequent dehalogenation by rhodococcus sp. strain dtb. | rhodococcus sp. strain dtb (dsm 44534) grows on bis(1-chloro-2-propyl) ether (dde) as sole source of carbon and energy. the non-chlorinated diisopropyl ether and bis(1-hydroxy-2-propyl) ether, however, did not serve as substrates. in ether degradation experiments with dense cell suspensions, 1-chloro-2-propanol and chloroacetone were formed, which indicated that scission of the ether bond is the first step while dehalogenation of the chlorinated c(3)-compounds occurs at a later stage of the degr ... | 2003 | 12605291 |
| highly enantioselective synthesis of alpha,alpha-disubstituted malonamic acids through asymmetric hydrolysis of dinitriles with rhodococcus sp. cgmcc 0497. | highly enantioselective hydrolysis of alpha,alpha-disubstituted malononitriles by the strain rhodococcus sp. cgmcc 0497 expressing both nitrile hydratase and amidase activity to give (r)-alpha,alpha-disubstituted malonamic acids which could be converted to valuable (r)- or (s)-alpha-alkylated amino acids are reported and the yields of the products are improved remarkably at a lower reaction temperature. | 2003 | 12613625 |
| qcrcab operon of a nocardia-form actinomycete rhodococcus rhodochrous encodes cytochrome reductase complex with diheme cytochrome cc subunit. | structural genes encoding quinol-cytochrome c reductase (qcr) were cloned and sequenced from nocardia-form actinomycete rhodococcus rhodochrous. qcrc and qcra encode diheme cytochrome cc and the rieske fe-s protein, respectively, while the qcrb product is a diheme cytochrome b. these amino acid sequences are similar to those of corynebacterium and mycobacterium, the members of high g+c content firmicutes. the presence of diheme cytochrome cc subunit as a sole c-type cytochrome in these organisms ... | 2003 | 12615356 |
| molecular cloning and characterization of an amidase from arabidopsis thaliana capable of converting indole-3-acetamide into the plant growth hormone, indole-3-acetic acid. | acylamidohydrolases from higher plants have not been characterized or cloned so far. atami1 is the first member of this enzyme family from a higher plant and was identified in the genome of arabidopsis thaliana based on sequence homology with the catalytic-domain sequence of bacterial acylamidohydrolases, particularly those that exhibit indole-3-acetamide amidohydrolase activity. atami1 polypeptide and mrna are present in leaf tissues, as shown by immunoblotting and rt-pcr, respectively. atami1 ... | 2003 | 12620340 |
| biotransformation of hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) by a rabbit liver cytochrome p450: insight into the mechanism of rdx biodegradation by rhodococcus sp. strain dn22. | a unique metabolite with a molecular mass of 119 da (c(2)h(5)n(3)o(3)) accumulated during biotransformation of hexahydro-1,3,5-trinitro-1,3,5-triazine (rdx) by rhodococcus sp. strain dn22 (d. fournier, a. halasz, j. c. spain, p. fiurasek, and j. hawari, appl. environ. microbiol. 68:166-172, 2002). the structure of the molecule and the reactions that led to its synthesis were not known. in the present study, we produced and purified the unknown metabolite by biotransformation of rdx with rhodococ ... | 2003 | 12620815 |
| synthesis of imidazol-2-yl amino acids by using cells from alkane-oxidizing bacteria. | sixty-one strains of alkane-oxidizing bacteria were tested for their ability to oxidize n-(2-hexylamino-4-phenylimidazol-1-yl)-acetamide to imidazol-2-yl amino acids applicable for pharmaceutical purposes. after growth with n-alkane, 15 strains formed different imidazol-2-yl amino acids identified by chemical structure analysis (mass and nuclear magnetic resonance spectrometry). high yields of imidazol-2-yl amino acids were produced by the strains gordonia rubropertincta sbug 105, gordonia terra ... | 2003 | 12620858 |
| [effects of nitriles and amides on the growth and the nitrile hydratase activity of the rhodococcus sp. strain gt1]. | effects of some nitriles and amides, as well as glucose and ammonium, on the growth and the nitrile hydratase (ec 4.2.1.84) activity of the rhodococcus sp. strain gt1 isolated from soil were studied. the activity of nitrile hydratase mainly depended on carbon and nitrogen supply to cells. the activity of nitrile hydratase was high in the presence of glucose and ammonium at medium concentrations and decreased at concentrations of glucose more than 0.3%. saturated unsubstituted aliphatic nitriles ... | 2003 | 12625044 |
| clearance of virulent but not avirulent rhodococcus equi from the lungs of adult horses is associated with intracytoplasmic gamma interferon production by cd4+ and cd8+ t lymphocytes. | rhodococcus equi is a gram-positive bacterium that infects alveolar macrophages and causes rhodococcal pneumonia in horses and humans. the virulence plasmid of r. equi appears to be required for both pathogenicity in the horse and the induction of protective immunity. an understanding of the mechanisms by which virulent r. equi circumvents protective host responses and by which bacteria are ultimately cleared is important for development of an effective vaccine. six adult horses were challenged ... | 2003 | 12626444 |
| performance of five serological assays for diagnosis of rhodococcus equi pneumonia in foals. | the performance of four enzyme-linked immunosorbent assays (elisas) (elisa-6939, elisa-33701, elisa-vapa, and elisa-california) and an agar gel immunodiffusion test for diagnosis of rhodococcus equi pneumonia in foals was evaluated. antibody concentrations of foals with culture-confirmed r. equi pneumonia (n = 41) were compared to those of age-matched pasturemates that remained clinically healthy during the entire breeding season (n = 24). for each serological assay evaluated, selection of a low ... | 2003 | 12626449 |
| practical synthesis of optically active alpha,alpha-disubstituted malonamic acids through asymmetric hydrolysis of malonamide derivatives with rhodococcus sp. cgmcc 0497. | a variety of alpha,alpha-disubstituted malonamides undergo enantioselective hydrolysis with rhodococcus sp. cgmcc 0497 to give challenging enantiopure alpha,alpha-disubstituted malonamic acids with up to >99% enantiomeric excesses and 98% chemical yields. the enantioselectivity originated from the effects of a highly enantioselective amidase. the products could be converted to valuable (r)- or (s)-alpha,alpha-dialkylated amino acids after routine conversions. | 2003 | 12636421 |
| the use of chemical and molecular microbial indicators for faecal source identification. | identifying the source of faecal pollution is important to enable appropriate management of faecal pollution of water. we are developing and evaluating a combination of these microbial and chemical indicators better able to identify the source of faecal pollution. these assays make use of a combination of direct pcr, culturing, and colony hybridisation to identify source specific species of bifidobacterium, rhodococcus and bacteroides. in conjunction with assays for (a) fluorescent whitening age ... | 2003 | 12639003 |
| activities of abt-773 against listeria monocytogenes and coryneform bacteria of clinical interest. | the in vitro activities of abt-773 were evaluated against 15 listeria monocytogenes strains and 196 coryneform bacteria isolated from clinical samples. one hundred percent of the l. monocytogenes strains were inhibited by </=0.015 micro g of abt-773/ml. mics of abt-773 ( micro g/ml) at which 50% of the isolates tested were inhibited (mic(50)s) and mic(90)s for other organisms were 0.125 and 0.5 (corynebacterium amycolatum), 1 and >32 (corynebacterium jeikeium), 0.03 and >32 (corynebacterium minu ... | 2003 | 12654678 |
| pathogenic nocardia, rhodococcus, and related organisms are highly susceptible to imidazole antifungals. | rhodococcus equi and species of nocardia and gordonia may be human opportunistic pathogens. we find that these, as well as several isolates from closely related genera, are highly susceptible to the imidazoles bifonazole, clotrimazole, econazole, and miconazole, whose mics are </=1 micro g/ml. in liquid cultures 1 micro g of the drug/ml was bacteriostatic and 10 micro g/ml was bactericidal. on solid media at 10 micro g of azole/ml no resistant mutants could be isolated. an mic of 1 to 15 micro g ... | 2003 | 12654698 |
| shifts in diversity and microscale distribution of the adapted bacterial phenotypes due to hg(ii) spiking in soil. | in a previous experiment [ranjard et al. (2000) fems microbiol ecol 31:107-115], the spatial heterogeneity of a mercury impact on soil bacterial community was revealed by an increase of mercury-resistant (hgr) bacterial numbers in the outer fraction and the sand fractions when compared to those in the silt fractions. the objectives of the present study were (i) to investigate whether mercury exposure affects the diversity and the distribution within the various fractions of the hgr populations a ... | 2003 | 12658524 |
| [laryngeal infection by rhodococcus equi in patient with aids]. | the rhodococcus equi is an aerobic gram positive pleomorphic bacillus, that was isolated for the first time like a producer of bronchopneumonia in young horses. every time more often, it is being recognized as a pathogen in humans, mainly in the immunodepressed population. we described a case, until now exceptional, of laryngeal infection by rhodococcus equi in a patient with positive serology for the virus of the human immunodeficiency (hiv), and we reviewed some clinical and epidemiological ch ... | 2002 | 12658846 |
| crystal structure of a histidine-tagged serine hydrolase involved in the carbazole degradation (carc enzyme). | 2-hydroxy-6-oxo-6-(2(')-aminophenyl)-hexa-2,4-dienoate hydrolases (carc enzymes) from two carbazole-degrading bacteria were purified using recombinant escherichia coli strains with the histidine (his)-tagged purification system. the his-tagged carc (ht-carc) enzymes from pseudomonas resinovorans strain ca10 (ht-carc(ca10)) and janthinobacterium sp. strain j3 (ht-carc(j3)) exhibited hydrolase activity toward 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate as the purified native carc(ca10) did. ht-carc( ... | 2003 | 12659866 |
| rhodococcus equi pneumonia in a heart transplant recipient in korea, with emphasis on microbial diagnosis. | rhodococcus equi is an opportunistic pathogen that usually causes infection in immunocompromised hosts. a heart transplant recipient who had been treated with amphotericin b for pulmonary aspergillosis showed newly developed multiple nodules with a central necrotic area in the right lower lobes. cultures of several blood samples and an aspirate of the lung nodule yielded a gram-positive coccobacillary bacterium, which was initially reported as a corynebacterium species, but was later identified ... | 2003 | 12667256 |
| phenotypic mutants of the intracellular actinomycete rhodococcus equi created by in vivo himar1 transposon mutagenesis. | rhodococcus equi is a facultative intracellular opportunistic pathogen of immunocompromised people and a major cause of pneumonia in young horses. an effective live attenuated vaccine would be extremely useful in the prevention of r. equi disease in horses. toward that end, we have developed an efficient transposon mutagenesis system that makes use of a himar1 minitransposon delivered by a conditionally replicating plasmid for construction of r. equi mutants. we show that himar1 transposition in ... | 2003 | 12670990 |
| evaluation of white blood cell concentration, plasma fibrinogen concentration, and an agar gel immunodiffusion test for early identification of foals with rhodococcus equi pneumonia. | to evaluate wbc concentration, plasma fibrinogen concentration, and an agar gel immunodiffusion (agid) test for early identification of rhodococcus equi-infected foals. | 2003 | 12675301 |
| spatial and temporal analysis of the microbial community in slow sand filters used for treating horticultural irrigation water. | an experimental slow sand filter (ssf) was constructed to study the spatial and temporal structure of a bacterial community suppressive to an oomycete plant pathogen, phytophthora cryptogea. passage of water through the mature sand column resulted in complete removal of zoospores of the plant pathogen. to monitor global changes in the microbial community, bacterial and fungal numbers were estimated on selective media, direct viable counts of fungal spores were made, and the atp content was measu ... | 2003 | 12676691 |
| evaluation of a commercially available hyperimmune plasma product for prevention of naturally acquired pneumonia caused by rhodococcus equi in foals. | to determine efficacy of a commercially available hyperimmune plasma product for prevention of naturally acquired pneumonia caused by rhodococcus equi in foals. | 2002 | 12680449 |