| comprehensive discovery and characterization of small rnas in corynebacterium glutamicum atcc 13032. | recent discoveries on bacterial transcriptomes gave evidence that small rnas (srnas) have important regulatory roles in prokaryotic cells. modern high-throughput sequencing approaches (rna-seq) enable the most detailed view on transcriptomes offering an unmatched comprehensiveness and single-base resolution. whole transcriptome data obtained by rna-seq can be used to detect and characterize all transcript species, including small rnas. here, we describe an rna-seq approach for comprehensive dete ... | 2013 | 24138339 |
| transcriptome and gene ontology (go) enrichment analysis reveals genes involved in biotin metabolism that affect l-lysine production in corynebacterium glutamicum. | corynebacterium glutamicum is widely used for amino acid production. in the present study, 543 genes showed a significant change in their mrna expression levels in l-lysine-producing c. glutamicum atcc21300 than that in the wild-type c. glutamicum atcc13032. among these 543 differentially expressed genes (degs), 28 genes were up- or downregulated. in addition, 454 degs were functionally enriched and categorized based on blast sequence homologies and gene ontology (go) annotations using the blast ... | 2016 | 27005618 |
| identification and characterization of γ-aminobutyric acid uptake system gabpcg (ncgl0464) in corynebacterium glutamicum. | corynebacterium glutamicum is widely used for industrial production of various amino acids and vitamins, and there is growing interest in engineering this bacterium for more commercial bioproducts such as γ-aminobutyric acid (gaba). in this study, a c. glutamicum gaba-specific transporter (gabp(cg)) encoded by ncgl0464 was identified and characterized. gabp(cg) plays a major role in gaba uptake and is essential to c. glutamicum growing on gaba. gaba uptake by gabp(cg) was weakly competed by l-as ... | 2012 | 22307305 |
| identification of two mutations increasing the methanol tolerance of corynebacterium glutamicum. | methanol is present in most ecosystems and may also occur in industrial applications, e.g. as an impurity of carbon sources such as technical glycerol. methanol often inhibits growth of bacteria, thus, methanol tolerance may limit fermentative production processes. | 2015 | 26474849 |
| characterization of fructose 1,6-bisphosphatase and sedoheptulose 1,7-bisphosphatase from the facultative ribulose monophosphate cycle methylotroph bacillus methanolicus. | the genome of the facultative ribulose monophosphate (rump) cycle methylotroph bacillus methanolicus encodes two bisphosphatases (glpx), one on the chromosome (glpx(c)) and one on plasmid pbm19 (glpx(p)), which is required for methylotrophy. both enzymes were purified from recombinant escherichia coli and were shown to be active as fructose 1,6-bisphosphatases (fbpases). the fbpase-negative corynebacterium glutamicum δfbp mutant could be phenotypically complemented with glpx(c) and glpx(p) from ... | 2013 | 24013630 |
| a prophage-encoded actin-like protein required for efficient viral dna replication in bacteria. | in host cells, viral replication is localized at specific subcellular sites. viruses that infect eukaryotic and prokaryotic cells often use host-derived cytoskeletal structures, such as the actin skeleton, for intracellular positioning. here, we describe that a prophage, cgp3, integrated into the genome of corynebacterium glutamicum encodes an actin-like protein, alpc. biochemical characterization confirms that alpc is a bona fide actin-like protein and cell biological analysis shows that alpc f ... | 2015 | 25916847 |
| fast mechanically driven daughter cell separation is widespread in actinobacteria. | dividing cells of the coccoid gram-positive bacterium staphylococcus aureus undergo extremely rapid (millisecond) daughter cell separation (dcs) driven by mechanical crack propagation, a strategy that is very distinct from the gradual, enzymatically driven cell wall remodeling process that has been well described in several rod-shaped model bacteria. to determine if other bacteria, especially those in the same phylum (firmicutes) or with similar coccoid shapes as s. aureus, might use a similar m ... | 2016 | 27578753 |
| dna cleavage by cgii and ngoavii requires interaction between n- and r-proteins and extensive nucleotide hydrolysis. | the stress-sensitive restriction-modification (rm) system cgli from corynebacterium glutamicum and the homologous ngoavii rm system from neisseria gonorrhoeae fa1090 are composed of three genes: a dna methyltransferase (m.cgli and m.ngoavii), a putative restriction endonuclease (r.cgli and r.ngoavii, or r-proteins) and a predicted dead-family helicase/atpase (n.cgli and n.ngoavii or n-proteins). here we report a biochemical characterization of the r- and n-proteins. size-exclusion chromatography ... | 2014 | 25429977 |
| application of den refinement and automated model building to a difficult case of molecular-replacement phasing: the structure of a putative succinyl-diaminopimelate desuccinylase from corynebacterium glutamicum. | phasing by molecular replacement remains difficult for targets that are far from the search model or in situations where the crystal diffracts only weakly or to low resolution. here, the process of determining and refining the structure of cgl1109, a putative succinyl-diaminopimelate desuccinylase from corynebacterium glutamicum, at ∼3 å resolution is described using a combination of homology modeling with modeller, molecular-replacement phasing with phaser, deformable elastic network (den) refi ... | 2012 | 22505259 |
| elucidation of a protein-protein interaction network involved in corynebacterium glutamicum cell wall biosynthesis as determined by bacterial two-hybrid analysis. | mycobacterium species have a highly complex and unique cell wall that consists of a large macromolecular structure termed the mycolyl-arabinogalactan-peptidoglycan (magp) complex. this complex is essential for growth, survival and virulence of the human pathogen mycobacterium tuberculosis, and is the target of several anti-tubercular drugs. the closely related species corynebacterium glutamicum has proven useful in the study of orthologous m. tuberculosis genes and proteins involved in magp synt ... | 2014 | 25117516 |
| engineering corynebacterium glutamicum for violacein hyper production. | corynebacterium glutamicum was used as a metabolic engineering chassis for production of crude violacein (mixture of violacein and deoxyviolacein) due to corynebacterium's gras status and advantages in tryptophan fermentation. the violacein is a commercially potential compound with various applications derived from l-tryptophan. | 2016 | 27557730 |
| metabolic engineering of corynebacterium glutamicum aimed at alternative carbon sources and new products. | corynebacterium glutamicum is well known as the amino acid-producing workhorse of fermentation industry, being used for multi-million-ton scale production of glutamate and lysine for more than 60 years. however, it is only recently that extensive research has focused on engineering it beyond the scope of amino acids. meanwhile, a variety of corynebacterial strains allows access to alternative carbon sources and/or allows production of a wide range of industrially relevant compounds. some of thes ... | 2012 | 24688664 |
| reductive whole-cell biotransformation with corynebacterium glutamicum: improvement of nadph generation from glucose by a cyclized pentose phosphate pathway using pfka and gapa deletion mutants. | in this study, the potential of corynebacterium glutamicum for reductive whole-cell biotransformation is shown. the nadph-dependent reduction of the prochiral methyl acetoacetate (maa) to the chiral (r)-methyl 3-hydroxybutyrate (mhb) by an alcohol dehydrogenase from lactobacillus brevis (lbadh) was used as model reaction and glucose served as substrate for the regeneration of nadph. since nadph is mainly formed in the oxidative branch of the pentose phosphate pathway (ppp), c. glutamicum was eng ... | 2012 | 22851018 |
| overexpression of genes encoding glycolytic enzymes in corynebacterium glutamicum enhances glucose metabolism and alanine production under oxygen deprivation conditions. | we previously reported that corynebacterium glutamicum strain δldhaδppc+alad+gapa, overexpressing glyceraldehyde-3-phosphate dehydrogenase-encoding gapa, shows significantly improved glucose consumption and alanine formation under oxygen deprivation conditions (t. jojima, m. fujii, e. mori, m. inui, and h. yukawa, appl. microbiol. biotechnol. 87:159-165, 2010). in this study, we employ stepwise overexpression and chromosomal integration of a total of four genes encoding glycolytic enzymes (herei ... | 2012 | 22504802 |
| an in silico platform for the design of heterologous pathways in nonnative metabolite production. | microorganisms are used as cell factories to produce valuable compounds in pharmaceuticals, biofuels, and other industrial processes. incorporating heterologous metabolic pathways into well-characterized hosts is a major strategy for obtaining these target metabolites and improving productivity. however, selecting appropriate heterologous metabolic pathways for a host microorganism remains difficult owing to the complexity of metabolic networks. hence, metabolic network design could benefit grea ... | 2012 | 22578364 |
| engineering the glycolytic pathway: a potential approach for improvement of biocatalyst performance. | the glycolytic pathway is a main driving force in the fermentation process as it produces energy, cell component precursors, and fermentation products. given its importance, the glycolytic pathway can be considered as an attractive target for the metabolic engineering of industrial microorganisms. however, many attempts to enhance glycolytic flux, by overexpressing homologous or heterologous genes encoding glycolytic enzymes, have been unsuccessful. in contrast, significant enhancement in glycol ... | 2015 | 26513591 |
| metabolic engineering of an atp-neutral embden-meyerhof-parnas pathway in corynebacterium glutamicum: growth restoration by an adaptive point mutation in nadh dehydrogenase. | corynebacterium glutamicum uses the embden-meyerhof-parnas pathway of glycolysis and gains 2 mol of atp per mol of glucose by substrate-level phosphorylation (slp). to engineer glycolysis without net atp formation by slp, endogenous phosphorylating nad-dependent glyceraldehyde-3-phosphate dehydrogenase (gapdh) was replaced by nonphosphorylating nadp-dependent glyceraldehyde-3-phosphate dehydrogenase (gapn) from clostridium acetobutylicum, which irreversibly converts glyceraldehyde-3-phosphate (g ... | 2015 | 25576602 |
| evaluation of the food grade expression systems nice and psip for the production of 2,5-diketo-d-gluconic acid reductase from corynebacterium glutamicum. | 2,5-diketo-d-gluconic acid reductase (2,5-dkg reductase) catalyses the reduction of 2,5-diketo-d-gluconic acid (2,5-dkg) to 2-keto-l-gulonic acid (2-klg), a direct precursor (lactone) of l-ascorbic acid (vitamin c). this reaction is an essential step in the biocatalytic production of the food supplement vitamin c from d-glucose or d-gluconic acid. as 2,5-dkg reductase is usually produced recombinantly, it is of interest to establish an efficient process for 2,5-dkg reductase production that also ... | 2013 | 23356419 |
| heterologous carotenoid-biosynthetic enzymes: functional complementation and effects on carotenoid profiles in escherichia coli. | a limited number of carotenoid pathway genes from microbial sources have been studied for analyzing the pathway complementation in the heterologous host escherichia coli. in order to systematically investigate the functionality of carotenoid pathway enzymes in e. coli, the pathway genes of carotenogenic microorganisms (brevibacterium linens, corynebacterium glutamicum, rhodobacter sphaeroides, rhodobacter capsulatus, rhodopirellula baltica, and pantoea ananatis) were modified to form synthetic e ... | 2013 | 23144136 |
| carotenoid biosynthesis and overproduction in corynebacterium glutamicum. | corynebacterium glutamicum contains the glycosylated c50 carotenoid decaprenoxanthin as yellow pigment. starting from isopentenyl pyrophosphate, which is generated in the non-mevalonate pathway, decaprenoxanthin is synthesized via the intermediates farnesyl pyrophosphate, geranylgeranyl pyrophosphate, lycopene and flavuxanthin. | 2012 | 22963379 |
| production of the marine carotenoid astaxanthin by metabolically engineered corynebacterium glutamicum. | astaxanthin, a red c40 carotenoid, is one of the most abundant marine carotenoids. it is currently used as a food and feed additive in a hundred-ton scale and is furthermore an attractive component for pharmaceutical and cosmetic applications with antioxidant activities. corynebacterium glutamicum, which naturally synthesizes the yellow c50 carotenoid decaprenoxanthin, is an industrially relevant microorganism used in the million-ton amino acid production. in this work, engineering of a genome-r ... | 2016 | 27376307 |
| corynebacterium jeikeium jk0268 constitutes for the 40 amino acid long poracj, which forms a homooligomeric and anion-selective cell wall channel. | corynebacterium jeikeium, a resident of human skin, is often associated with multidrug resistant nosocomial infections in immunodepressed patients. c. jeikeium k411 belongs to mycolic acid-containing actinomycetes, the mycolata and contains a channel-forming protein as judged from reconstitution experiments with artificial lipid bilayer experiments. the channel-forming protein was present in detergent treated cell walls and in extracts of whole cells using organic solvents. a gene coding for a 4 ... | 2013 | 24116064 |
| corynebacterium glutamicum methionine sulfoxide reductase a uses both mycoredoxin and thioredoxin for regeneration and oxidative stress resistance. | oxidation of methionine leads to the formation of the s and r diastereomers of methionine sulfoxide (meto), which can be reversed by the actions of two structurally unrelated classes of methionine sulfoxide reductase (msr), msra and msrb, respectively. although msras have long been demonstrated in numerous bacteria, their physiological and biochemical functions remain largely unknown in actinomycetes. here, we report that a corynebacterium glutamicum methionine sulfoxide reductase a (cgmsra) tha ... | 2015 | 25681179 |
| engineering corynebacterium glutamicum for the production of 2,3-butanediol. | 2,3-butanediol is an important bulk chemical with a wide range of applications. in bacteria, this metabolite is synthesised from pyruvate via a three-step pathway involving α-acetolactate synthase, α-acetolactate decarboxylase and 2,3-butanediol dehydrogenase. thus far, the best producers of 2,3-butanediol are pathogenic strains, hence, the development of more suitable organisms for industrial scale fermentation is needed. herein, 2,3-butanediol production was engineered in the generally regarde ... | 2015 | 26511723 |
| metabolic pathway engineering for production of 1,2-propanediol and 1-propanol by corynebacterium glutamicum. | production of the versatile bulk chemical 1,2-propanediol and the potential biofuel 1-propanol is still dependent on petroleum, but some approaches to establish bio-based production from renewable feed stocks and to avoid toxic intermediates have been described. the biotechnological workhorse corynebacterium glutamicum has also been shown to be able to overproduce 1,2-propanediol by metabolic engineering. additionally, c. glutamicum has previously been engineered for production of the biofuels e ... | 2015 | 26110019 |
| identification and methionine analog tolerance of environmental bacterial isolates selected on methionine analog containing medium. | methionine is the first limiting amino acid in poultry feed. currently, methionine supplement is synthesized from an expensive chemical process requiring hazardous chemicals. therefore, the objectives of this study were isolation of methionine producing bacteria from environmental samples and quantification of methionine production in these isolated bacteria. mcgc medium was selected as the isolation medium for methionine-producing bacteria by using corynebacterium glutamicum atcc13032 and esche ... | 2014 | 24502216 |
| increasing the antibacterial activity of gentamicin in combination with extracted polyphosphate from bacillus megaterium. | the aim of this research was production of polyphosphate (poly p) and study on its antibacterial effects. | 2013 | 23332009 |
| engineering of corynebacterium glutamicum for xylitol production from lignocellulosic pentose sugars. | xylitol is a non-fermentable sugar alcohol used as sweetener. corynebacterium glutamicum atcc13032 was metabolically engineered for xylitol production from the lignocellulosic pentose sugars xylose and arabinose. direct conversion of xylose to xylitol was achieved through the heterologous expression of nad(p)h-dependent xylose reductase (xr) gene from rhodotorula mucilaginosa. xylitol synthesis from arabinose was attained through polycistronic expression of l-arabinose isomerase (araa), d-psicos ... | 2016 | 27184428 |
| tatc-dependent translocation of pyoverdine is responsible for the microbial growth suppression. | infections are often not caused by a colonization of pseudomonas aeruginosa alone but by a consortium of other bacteria. little is known about the impact of p. aeruginosa on the growth of other bacteria upon coinfection. here, cell-ree culture supernatants obtained from p. aeruginosa suppressed the growth of a number of bacterial strains such as corynebacterium glutamicum, bacillus subtilis, staphylococcus aureus, and agrobacterium tumefaciens, but had little effect on the growth of escherichia ... | 2016 | 26832668 |
| metabolic engineering of corynebacterium glutamicum for the production of itaconate. | the capability of corynebacterium glutamicum for glucose-based synthesis of itaconate was explored, which can serve as building block for production of polymers, chemicals, and fuels. c. glutamicum was highly tolerant to itaconate and did not metabolize it. expression of the aspergillus terreus cad1 gene encoding cis-aconitate decarboxylase (cad) in strain atcc13032 led to the production of 1.4mm itaconate in the stationary growth phase. fusion of cad with the escherichia coli maltose-binding pr ... | 2015 | 26100077 |
| metabolic engineering of itaconate production in escherichia coli. | interest in sustainable development has led to efforts to replace petrochemical-based monomers with biomass-based ones. itaconic acid, a c5-dicarboxylic acid, is a potential monomer for the chemical industry with many prospective applications. cis-aconitate decarboxylase (cada) is the key enzyme of itaconate production, converting the citric acid cycle intermediate cis-aconitate into itaconate. heterologous expression of cada from aspergillus terreus in escherichia coli resulted in low cada acti ... | 2015 | 25277412 |
| high-level secretory production of recombinant single-chain variable fragment (scfv) in corynebacterium glutamicum. | we describe the development of a new secretory production system for the enhanced production of a single-chain variable fragment (scfv) against the anthrax toxin in corynebacterium glutamicum. for efficient secretory production of the antibody fragment, the following components were examined: (1) signal peptides, (2) codon usage of antibody fragment, (3) promoters, (4) 5' untranslated region (5' utr) sequence, and (5) transcriptional terminator. among all the systems examined, the use of a codon ... | 2014 | 24380967 |
| high-level production of bacillus cereus phospholipase c in corynebacterium glutamicum. | enzymatic oil degumming (removal of phospholipids) using phospholipase c (plc) is a well-established and environmentally friendly process for vegetable oil refining. in this work, we report the production of recombinant bacillus cereus plc in corynebacterium glutamicum atcc 13869 in a high cell density fermentation process and its performance in soybean oil degumming. a final concentration of 5.5g/l of the recombinant enzyme was achieved when the respective gene was expressed from the tac promot ... | 2015 | 26519562 |
| extensive exometabolome analysis reveals extended overflow metabolism in various microorganisms. | overflow metabolism is well known for yeast, bacteria and mammalian cells. it typically occurs under glucose excess conditions and is characterized by excretions of by-products such as ethanol, acetate or lactate. this phenomenon, also denoted the short-term crabtree effect, has been extensively studied over the past few decades, however, its basic regulatory mechanism and functional role in metabolism is still unknown. here we present a comprehensive quantitative and time-dependent analysis of ... | 2012 | 22963408 |
| impact of carbon source and variable nitrogen conditions on bacterial biosynthesis of polyhydroxyalkanoates: evidence of an atypical metabolism in bacillus megaterium dsm 509. | twenty bacterial strains were examined on their ability to produce polyhydroxyalkanoates (pha) from different carbon sources under rich and depleted nitrogen conditions. preliminary experiments with glucose as sole carbon source allowed to select pha producing bacteria using ftir spectroscopy. they were further tested with eight additional carbon substrates including organic, fatty acids or sugars. pha content and monomer composition of four chosen strains (pseudomonas putida mt-2, bacillus mega ... | 2013 | 23548274 |
| an assay for functional xylose transporters in saccharomyces cerevisiae. | it has been considered that more efficient uptake of xylose could promote increased xylose metabolic capacity of several microorganisms. in this study, an assay to screen xylose transporters was established in the saccharomyces cerevisiae strain, which expresses the xylosidase gene of bacillus pumilus intracellularly. the absorbed xylose analog p-nitrophenyl-β-d-xylopyranoside (pnpx) rapidly hydrolyzed to p-nitrophenol (pnp), which displayed a yellow tint when exposed to xylosidase in vivo. the ... | 2013 | 23928049 |
| enhanced poly(3-hydroxypropionate) production via β-alanine pathway in recombinant escherichia coli. | poly(3-hydroxypropionate) (p3hp) is a thermoplastic with great compostability and biocompatibility, and can be produced through several biosynthetic pathways, in which the glycerol pathway achieved the highest p3hp production. however, exogenous supply of vitamin b12 was required to maintain the activity of glycerol dehydratase, resulting in high production cost. to avoid the addition of vb12, we have previously constructed a p3hp biosynthetic route with β-alanine as intermediate, and the presen ... | 2017 | 28253372 |
| bacterial genome editing via a designed toxin-antitoxin cassette. | manipulating the bacterial genomes in an efficient manner is essential to biological and biotechnological research. here, we reprogrammed the bacterial ta systems as the toxin counter-selectable cassette regulated by an antitoxin switch (tccras) for genetic modifications in the extensively studied and utilized gram-positive bacteria, b. subtilis and corynebacterium glutamicum. in the five characterized type ii ta systems, the relbe complex can specifically and efficiently regulate cell growth an ... | 2017 | 28094982 |
| use of a sec signal peptide library from bacillus subtilis for the optimization of cutinase secretion in corynebacterium glutamicum. | technical bulk enzymes represent a huge market, and the extracellular production of such enzymes is favorable due to lowered cost for product recovery. protein secretion can be achieved via general secretion (sec) pathway. specific sequences, signal peptides (sps), are necessary to direct the target protein into the translocation machinery. for example, >150 sec-specific sps have been identified for bacillus subtilis alone. as the best sp for a target protein of choice cannot be predicted a prio ... | 2016 | 27927208 |
| engineering corynebacterium glutamicum for fast production of l-lysine and l-pipecolic acid. | the gram-positive corynebacterium glutamicum is widely used for fermentative production of amino acids. the world production of l-lysine has surpassed 2 million tons per year. glucose uptake and phosphorylation by c. glutamicum mainly occur by the phosphotransferase system (pts) and to lesser extent by inositol permeases and glucokinases. heterologous expression of the genes for the high-affinity glucose permease from streptomyces coelicolor and bacillus subtilis glucokinase fully compensated fo ... | 2016 | 27345060 |
| identification of d-amino acid dehydrogenase as an upstream regulator of the autoinduction of a putative acyltransferase in corynebacterium glutamicum. | expression of a putative acyltransferase encoded by ncgl- 0350 of corynebacterium glutamicum is induced by cell-free culture fluids obtained from stationary-phase growth of both c. glutamicum and pseudomonas aeruginosa, providing evidence for interspecies communication. here, we further confirmed that such communication occurs by showing that acyltransferase expression is induced by culture fluid obtained from diverse gram-negative and -positive bacterial strains, including escherichia coli, sal ... | 2016 | 27225460 |
| diverse effects of a biosurfactant from rhodococcus ruber iegm 231 on the adhesion of resting and growing bacteria to polystyrene. | this study evaluated the effects of a trehalolipid biosurfactant produced by rhodococcus ruber iegm 231 on the bacterial adhesion and biofilm formation on the surface of polystyrene microplates. the adhesion of gram-positive (arthrobacter simplex, bacillus subtilis, brevibacterium linens, corynebacterium glutamicum, micrococcus luteus) and gram-negative (escherichia coli, pseudomonas fluorescencens) bacteria correlated differently with the cell hydrophobicity and surface charge. in particular, e ... | 2016 | 26888203 |
| [characterization of l-aspartate-α-decarboxylase from bacillus subtilis]. | as an important material in pharmaceutical and chemical industry, β-alanine was mainly produced by chemical methods. l-aspartate-α-decarboxylase could catalyze the α-decarboxylation from l-aspartate to β-alanine. determinations for specific activities of pands from escherichia coli, corynebacterium glutamicum and bacillus subtilis were performed in this study (0.98 u/mg, 7.52 u/mg and 8.4 u/mg respectively). the optimal temperature and ph of pands from c. glutamicum and b. subtilis were 65 degre ... | 2015 | 26762040 |
| a tatabc-type tat translocase is required for unimpaired aerobic growth of corynebacterium glutamicum atcc13032. | the twin-arginine translocation (tat) system transports folded proteins across the cytoplasmic membrane of bacteria and the thylakoid membrane of plant chloroplasts. escherichia coli and other gram-negative bacteria possess a tatabc-type tat translocase in which each of the three inner membrane proteins tata, tatb, and tatc performs a mechanistically distinct function. in contrast, low-gc gram-positive bacteria, such as bacillus subtilis, use a tatac-type minimal tat translocase in which the tat ... | 2015 | 25837592 |
| exploring lysine riboswitch for metabolic flux control and improvement of l-lysine synthesis in corynebacterium glutamicum. | riboswitch, a regulatory part of an mrna molecule that can specifically bind a metabolite and regulate gene expression, is attractive for engineering biological systems, especially for the control of metabolic fluxes in industrial microorganisms. here, we demonstrate the use of lysine riboswitch and intracellular l-lysine as a signal to control the competing but essential metabolic by-pathways of lysine biosynthesis. to this end, we first examined the natural lysine riboswitches of eschericia co ... | 2015 | 25575181 |
| a novel approach for improving the yield of bacillus subtilis transglutaminase in heterologous strains. | the transglutaminase (btg) from bacillus subtilis is considered to be a new type of transglutaminase for the food industry. given that the btg gene only encodes a mature peptide, the expression of btg in heterologous microbial hosts could affect their normal growth due to btg's typical transglutaminase activity which can catalyze cross-linking of proteins in the cells. therefore, we developed a novel approach to suppress btg activity and reduce the toxicity on microbial hosts, thus improving btg ... | 2014 | 24947581 |
| analysis of cepa encoding an efflux pump-like protein in corynebacterium glutamicum. | a gene encoding a homolog of purine efflux proteins of escherichia coli and bacillus subtilis was identified in the genome of corynebacterium glutamicum and designated as cepa. the gene encoded a putative protein product, containing 12 transmembrane helixes, which is a typical feature of integral membrane transport proteins. to elucidate the function of the gene, we constructed a cepa deletion mutant (δcepa) and a cepa-overexpressing strain and analyzed their physiological characteristics. the c ... | 2014 | 24535744 |
| engineering biotin prototrophic corynebacterium glutamicum strains for amino acid, diamine and carotenoid production. | the gram-positive corynebacterium glutamicum is auxotrophic for biotin. besides the biotin uptake system bioymn and the transcriptional regulator bioq, this bacterium possesses functional enzymes for the last three reactions of biotin synthesis starting from pimeloyl-coa. heterologous expression of biof from the gram-negative escherichia coli enabled biotin synthesis from pimelic acid added to the medium, but expression of biof together with bioc and bioh from e. coli did not entail biotin proto ... | 2014 | 24486440 |
| enhancement of l-ornithine production by disruption of three genes encoding putative oxidoreductases in corynebacterium glutamicum. | recently, corynebacterium glutamicum has been shown to exhibit gluconate bypass activity, with two key enzymes, glucose dehydrogenase (gdh) and gluconate kinase, that provides an alternate route to 6-phosphogluconate formation. in this study, gene disruption analysis was used to examine possible metabolic functions of three proteins encoded by open reading frames having significant sequence similarity to gdh of bacillus subtilis. chromosomal in-frame deletion of three genes (ncgl0281, ncgl2582, ... | 2014 | 24402505 |
| the lipid ii flippase roda determines morphology and growth in corynebacterium glutamicum. | lipid ii flippases play an essential role in cell growth and the maintenance of cell shape in many rod-shaped bacteria. the putative lipid ii flippase roda is an integral membrane protein and member of the seds (shape, elongation, division and sporulation) protein family. in contrast to its homologues in escherichia coli and bacillus subtilis little is known about the role of roda in actinobacteria. in this study, we describe the localization and function of roda in corynebacterium glutamicum, a ... | 2013 | 24118443 |
| metabolic engineering of corynebacterium glutamicum for increasing the production of l-ornithine by increasing nadph availability. | the experiments presented here were based on the conclusions of our previous proteomic analysis. increasing the availability of glutamate by overexpression of the genes encoding enzymes in the l-ornithine biosynthesis pathway upstream of glutamate and disruption of spee, which encodes spermidine synthase, improved l-ornithine production by corynebacterium glutamicum. production of l-ornithine requires 2 moles of nadph per mole of l-ornithine. thus, the effect of nadph availability on l-ornithine ... | 2013 | 23836141 |
| development of biotin-prototrophic and -hyperauxotrophic corynebacterium glutamicum strains. | to develop the infrastructure for biotin production through naturally biotin-auxotrophic corynebacterium glutamicum, we attempted to engineer the organism into a biotin prototroph and a biotin hyperauxotroph. to confer biotin prototrophy on the organism, the cotranscribed biobf genes of escherichia coli were introduced into the c. glutamicum genome, which originally lacked the biof gene. the resulting strain still required biotin for growth, but it could be replaced by exogenous pimelic acid, a ... | 2013 | 23709504 |
| engineering of acetate recycling and citrate synthase to improve aerobic succinate production in corynebacterium glutamicum. | corynebacterium glutamicum lacking the succinate dehydrogenase complex can produce succinate aerobically with acetate representing the major byproduct. efforts to increase succinate production involved deletion of acetate formation pathways and overexpression of anaplerotic pathways, but acetate formation could not be completely eliminated. to address this issue, we constructed a pathway for recycling wasted carbon in succinate-producing c. glutamicum. the acetyl-coa synthetase from bacillus sub ... | 2013 | 23593275 |
| picoliter ndep traps enable time-resolved contactless single bacterial cell analysis in controlled microenvironments. | we present a lab-on-a-chip device, the envirostat 2.0, which allows for the first time contactless cultivation of a single bacterial cell by negative dielectrophoresis (ndep) in a precisely controllable microenvironment. stable trapping in perfusing growth medium was achieved by a miniaturization of octupole electrode geometries, matching the dimensions of bacteria. temperature sensitive fluorescent measurements showed that these reductions of microelectrode distances led to reduced joule heatin ... | 2013 | 23223864 |
| glycerol-3-phosphatase of corynebacterium glutamicum. | formation of glycerol as by-product of amino acid production by corynebacterium glutamicum has been observed under certain conditions, but the enzyme(s) involved in its synthesis from glycerol-3-phosphate were not known. it was shown here that cg1700 encodes an enzyme active as a glycerol-3-phosphatase (gpp) hydrolyzing glycerol-3-phosphate to inorganic phosphate and glycerol. gpp was found to be active as a homodimer. the enzyme preferred conditions of neutral ph and requires mg²⁺ or mn²⁺ for i ... | 2012 | 22353596 |
| conservation of structure and mechanism within the transaldolase enzyme family. | transaldolase (tal) is involved in the central carbon metabolism, i.e. the non-oxidative pentose phosphate pathway, and is therefore a ubiquitous enzyme. however, tals show a low degree in sequence identity and vary in length within the enzyme family which previously led to the definition of five subfamilies. we wondered how this variation is conserved in structure and function. to answer this question we characterised and compared the tals from bacillus subtilis, corynebacterium glutamicum and ... | 2012 | 22212631 |
| 4-hydroxyisoleucine production of recombinant corynebacterium glutamicum ssp. lactofermentum under optimal corn steep liquor limitation. | 4-hydroxyisoleucine (4-hil) is a nonproteinogenic amino acid that exhibits insulinotropic biological activity. here, l-isoleucine dioxygenase gene (ido) derived from bacillus thuringiensis ybt-1520 was cloned and expressed in an l-isoleucine-producing strain, corynebacterium glutamicum ssp. lactofermentum sn01, in order to directly convert its endogenous l-isoleucine (ile) into 4-hil through single-step fermentation. the effects of corn steep liquor limitation as well as ido and truncated idoδ6 ... | 2015 | 25725632 |
| [expression optimization and characterization of tenebrio molitor antimicrobiol peptides tmamp1m in escherichia coli]. | to improve the expression level of tmamp1m gene from tenebrio molitor in escherichia coli, we studied the effects of expression level and activity of the fusion protein his-tmamp1m by conditions, such as culture temperature, inducing time and the final concentration of inductor isopropyl beta-d-thiogalactopyranoside (iptg). we analyzed the optimum expression conditions by tricine-sds-page electrophoresis, meanwhile, detected its antibacterial activity by using agarose cavity diffusion method. th ... | 2013 | 24063242 |
| construction and application of an expression vector from the new plasmid platc1 of acidithiobacillus caldus. | in this study, a recently sequenced 9.8-kb plasmid, platc1, from acidithiobacillus caldus strain sm-1 was characterized and developed into an expression vector. the platc1 backbone carried an oriv, three rep genes, five mob genes, a nic site, and an addiction system. multilocus sequence analysis indicated that platc1 was phylogenetically more related to the incq-like broad host range plasmids than to other incq plasmids. platc1 was able to replicate and reside in gram-negative escherichia coli, ... | 2014 | 24445921 |
| identification and characterization of the channel-forming protein in the cell wall of corynebacterium amycolatum. | the mycolic-acid layer of certain gram-positive bacteria, the mycolata, represents an additional permeability barrier for the permeation of small water-soluble solutes. consequently, it was shown in recent years that the mycolic acid layer of individual bacteria of the group mycolata contains pores, called porins, for the passage of hydrophilic solutes. corynebacterium amycolatum, a pathogenic corynebacterium species, belongs to the corynebacteriaceae family but it lacks corynomycolic acids in i ... | 2013 | 23811360 |
| comparing galactan biosynthesis in mycobacterium tuberculosis and corynebacterium diphtheriae. | the suborder corynebacterineae encompasses species like corynebacterium glutamicum, which has been harnessed for industrial production of amino acids, as well as corynebacterium diphtheriae and mycobacterium tuberculosis, which cause devastating human diseases. a distinctive component of the corynebacterineae cell envelope is the mycolyl-arabinogalactan (mag) complex. the mag is composed of lipid mycolic acids, and arabinofuranose (araf) and galactofuranose (galf) carbohydrate residues. elucidat ... | 2017 | 28039359 |
| impact of lytr-cpsa-psr proteins on cell wall biosynthesis in corynebacterium glutamicum. | proteins of the lcp (lytr, cpsa, psr) family have been shown to inherit important roles in bacterial cell wall biosynthesis. however, their exact function in the formation of the complex cell wall structures of the corynebacteriales, including the prominent pathogens mycobacterium tuberculosis and corynebacterium diphtheriae, remains unclear. here, we analyzed the role of the lcp proteins lcpa and lcpb of corynebacterium glutamicum, both of which localize at regions of nascent cell wall biosynth ... | 2016 | 27551018 |
| caenorhabditis elegans star formation and negative chemotaxis induced by infection with corynebacteria. | caenorhabditis elegans is one of the major model systems in biology based on advantageous properties such as short life span, transparency, genetic tractability and ease of culture using an escherichia coli diet. in its natural habitat, compost and rotting plant material, this nematode lives on bacteria. however, c. elegans is a predator of bacteria, but can also be infected by nematopathogenic coryneform bacteria such microbacterium and leucobacter species, which display intriguing and diverse ... | 2016 | 26490043 |
| acetylation of trehalose mycolates is required for efficient mmpl-mediated membrane transport in corynebacterineae. | pathogenic species of mycobacteria and corynebacteria, including mycobacterium tuberculosis and corynebacterium diphtheriae, synthesize complex cell walls that are rich in very long-chain mycolic acids. these fatty acids are synthesized on the inner leaflet of the cell membrane and are subsequently transported to the periplasmic space as trehalose monomycolates (tmm), where they are conjugated to other cell wall components and to tmm to form trehalose dimycolates (tdm). mycobacterial tmm, and th ... | 2015 | 25427102 |
| engineered coryneform bacteria as a bio-tool for arsenic remediation. | despite current remediation efforts, arsenic contamination in water sources is still a major health problem, highlighting the need for new approaches. in this work, strains of the nonpathogenic and highly arsenic-resistant bacterium corynebacterium glutamicum were used as inexpensive tools to accumulate inorganic arsenic, either as arsenate (as(v)) or arsenite (as(iii)) species. the assays made use of "resting cells" from these strains, which were assessed under well-established conditions and c ... | 2014 | 25208910 |
| ipsa, a novel laci-type regulator, is required for inositol-derived lipid formation in corynebacteria and mycobacteria. | the development of new drugs against tuberculosis and diphtheria is focused on disrupting the biogenesis of the cell wall, the unique architecture of which confers resistance against current therapies. the enzymatic pathways involved in the synthesis of the cell wall by these pathogens are well understood, but the underlying regulatory mechanisms are largely unknown. | 2013 | 24377418 |
| identification of a mycoloyl transferase selectively involved in o-acylation of polypeptides in corynebacteriales. | we have previously described the posttranslational modification of pore-forming small proteins of corynebacterium by mycolic acid, a very-long-chain α-alkyl and β-hydroxy fatty acid. using a combination of chemical analyses and mass spectrometry, we identified the mycoloyl transferase (myt) that catalyzes the transfer of the fatty acid residue to yield o-acylated polypeptides. inactivation of corynomycoloyl transferase c (cg0413 [corynebacterium glutamicum mytc {cgmytc}]), one of the six cgmyt g ... | 2013 | 23852866 |
| characterization of the microbiota in the guts of triatoma brasiliensis and triatoma pseudomaculata infected by trypanosoma cruzi in natural conditions using culture independent methods. | chagas disease is caused by trypanosoma cruzi, which is transmitted by triatomine vectors. the northeastern region of brazil is endemic for chagas disease and has the largest diversity of triatomine species. t. cruzi development in its triatomine vector depends on diverse factors, including the composition of bacterial gut microbiota. | 2015 | 25903360 |
| ubiquitous distribution of phosphatidylinositol phosphate synthase and archaetidylinositol phosphate synthase in bacteria and archaea, which contain inositol phospholipid. | in eukarya, phosphatidylinositol (pi) is biosynthesized from cdp-diacylglycerol (cdp-dag) and inositol. in archaea and bacteria, on the other hand, we found a novel inositol phospholipid biosynthetic pathway. the precursors, inositol 1-phosphate, cdp-archaeol (cdp-aroh), and cdp-dag, form archaetidylinositol phosphate (aip) and phosphatidylinositol phosphate (pip) as intermediates. these intermediates are dephosphorylated to synthesize archaetidylinositol (ai) and pi. to date, the activities of ... | 2014 | 24269814 |
| complete genome sequence of enterobacter cloacae ggt036: a furfural tolerant soil bacterium. | enterobacter cloacae is a facultative anaerobic bacterium to be an important cause of nosocomial infection. however, the isolated e. cloacae ggt036 showed higher furfural-tolerant cellular growth, compared to industrial relevant strains such as escherichia coli and corynebacterium glutamicum. here, we report the complete genome sequence of e. cloacae ggt036 isolated from mt. gwanak, seoul, republic of korea. the genomic dna sequence of e. cloacae ggt036 will provide valuable genetic resources fo ... | 2015 | 25444880 |
| extreme furfural tolerance of a soil bacterium enterobacter cloacae ggt036. | detoxification process of cellular inhibitors including furfural is essential for production of bio-based chemicals from lignocellulosic biomass. here we isolated an extreme furfural-tolerant bacterium enterobacter cloacae ggt036 from soil sample collected in mt. gwanak, republic of korea. among isolated bacteria, only e. cloacae ggt036 showed cell growth with 35 mm furfural under aerobic culture. compared to the maximal half inhibitory concentration (ic50) of well-known industrial strains esche ... | 2015 | 25444876 |
| development of indole-3-acetic acid-producing escherichia coli by functional expression of ipdc, aspc, and iad1. | biosynthesis of indole-3-acetic acid (iaa) via the indole-3-pyruvic acid pathway involves three kinds of enzymes; aminotransferase encoded by aspc, indole-3-pyruvic acid decarboxylase encoded by ipdc, and indole-3-acetic acid dehydrogenase encoded by iad1. the ipdc from enterobacter cloacae atcc 13047, aspc from escherichia coli, and iad1 from ustilago maydis were cloned and expressed under the control of the tac and sod promoters in e. coli. according to sds-page and enzyme activity, ipdc and i ... | 2013 | 24043123 |
| inactivation of genes involved in base excision repair of corynebacterium glutamicum and survival of the mutants in presence of various mutagens. | base excision repair (ber) is considered as the most active dna repair pathway in vivo, which is initiated by recognition of the nucleotide lesions and excision of the damaged dna base. the genome of corynebacterium glutamicum atcc 13032 contains various dna glycosylases encoding genes (ung, fpg/mutm, tagi, alka, muty), two ap-endonuclease encoding genes (nei and nth) and an exonuclease encoding gene xth. to investigate the role of these genes during dna repair in c. glutamicum, mutants with del ... | 2017 | 28391506 |
| heterologous expression of the halothiobacillus neapolitanus carboxysomal gene cluster in corynebacterium glutamicum. | compartmentalization represents a ubiquitous principle used by living organisms to optimize metabolic flux and to avoid detrimental interactions within the cytoplasm. proteinaceous bacterial microcompartments (bmcs) have therefore created strong interest for the encapsulation of heterologous pathways in microbial model organisms. however, attempts were so far mostly restricted to escherichia coli. here, we introduced the carboxysomal gene cluster of halothiobacillus neapolitanus into the biotech ... | 2017 | 28359868 |
| redirecting carbon flux through pgi-deficient and heterologous transhydrogenase toward efficient succinate production in corynebacterium glutamicum. | corynebacterium glutamicum is particularly known for its potentiality in succinate production. we engineered c. glutamicum for the production of succinate. to enhance c3-c4 carboxylation efficiency, chromosomal integration of the pyruvate carboxylase gene pyc resulted in strain nc-4. to increase intracellular nadh pools, the pntab gene from escherichia coli, encoding for transhydrogenase, was chromosomally integrated into nc-4, leading to strain nc-5. furthermore, we deleted pgi gene in strain n ... | 2017 | 28303352 |
| functional expression of plant-derived o-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin. | plant polyphenols receive significant attention due to their anti-oxidative and health-promoting properties, and several microorganisms are currently engineered towards producing these valuable compounds. previously, corynebacterium glutamicum has been engineered for synthesizing polyphenol core structures such as the stilbene resveratrol and the (2s)-flavanone naringenin. decoration of these compounds by o-methylation or hydroxylation would provide access to polyphenols of even higher commercia ... | 2017 | 28143765 |
| heat-killed cell preparation of corynebacterium glutamicum stimulates the immune activity and improves survival of mice against enterohemorrhagic escherichia coli. | fermentation by corynebacterium glutamicum is used by various industries to produce l-glutamate, and the heat-killed cell preparation of this bacterium (hccg) is a by-product of the fermentation process. in present study, we evaluated the immunostimulating and survival effects against enterohemorrhagic escherichia coli (stec) infection of hccg. hccg significantly stimulated in vitro iga and interleukin-12 p70 production in murine peyer's patch cells and peritoneal macrophages, respectively. oral ... | 2017 | 28137189 |
| biotin-independent strains of escherichia coli for enhanced streptavidin production. | biotin is an archetypal vitamin used as cofactor for carboxylation reactions found in all forms of life. however, biotin biosynthesis is an elaborate multi-enzymatic process and metabolically costly. moreover, many industrially relevant organisms are incapable of biotin synthesis resulting in the requirement to supplement defined media. here we describe the creation of biotin-independent strains of escherichia coli and corynebacterium glutamicum through installation of an optimized malonyl-coa b ... | 2017 | 28062280 |
| polynucleotide phosphorylase, rnase e/g, and ybey are involved in the maturation of 4.5s rna in corynebacterium glutamicum. | corynebacterium glutamicum has been applied for the industrial production of various metabolites, such as amino acids. to understand the biosynthesis of the membrane protein in this bacterium, we investigated the process of signal recognition particle (srp) assembly. srp is found in all three domains of life and plays an important role in the membrane insertion of proteins. srp rna is initially transcribed as precursor molecules; however, relatively little is known about its maturation. in c. gl ... | 2017 | 28031281 |
| branched-chain amino acids. | branched-chain amino acids (bcaas), viz., l-isoleucine, l-leucine, and l-valine, are essential amino acids that cannot be synthesized in higher organisms and are important nutrition for humans as well as livestock. they are also valued as synthetic intermediates for pharmaceuticals. therefore, the demand for bcaas in the feed and pharmaceutical industries is increasing continuously. traditional industrial fermentative production of bcaas was performed using microorganisms isolated by random muta ... | 2016 | 27872960 |
| biotechnological production of aromatic compounds of the extended shikimate pathway from renewable biomass. | aromatic chemicals that contain an unsaturated ring with alternating double and single bonds find numerous applications in a wide range of industries, e.g. paper and dye manufacture, as fuel additives, electrical insulation, resins, pharmaceuticals, agrochemicals, in food, feed and cosmetics. their chemical production is based on petroleum (btx; benzene, toluene, and xylene), but they can also be obtained from plants by extraction. due to petroleum depletion, health compliance, or environmental ... | 2016 | 27871872 |
| exporters for production of amino acids and other small molecules. | microbes are talented catalysts to synthesize valuable small molecules in their cytosol. however, to make full use of their skills - and that of metabolic engineers - the export of intracellularly synthesized molecules to the culture medium has to be considered. this step is as essential as is each step for the synthesis of the favorite molecule of the metabolic engineer, but is frequently not taken into account. to export small molecules via the microbial cell envelope, a range of different typ ... | 2016 | 27832297 |
| lysine fermentation: history and genome breeding. | lysine fermentation by corynebacterium glutamicum was developed in 1958 by kyowa hakko kogyo co. ltd. (current kyowa hakko bio co. ltd.) and is the second oldest amino acid fermentation process after glutamate fermentation. the fundamental mechanism of lysine production, discovered in the early stages of the process's history, gave birth to the concept known as "metabolic regulatory fermentation," which is now widely applied to metabolite production. after the development of rational metabolic e ... | 2016 | 27832296 |
| increased production of food-grade d-tagatose from d-galactose by permeabilized and immobilized cells of corynebacterium glutamicum, a gras host, expressing d-galactose isomerase from geobacillus thermodenitrificans. | the generally recognized as safe microorganism corynebacterium glutamicum expressing geobacillus thermodenitrificans d-galactose isomerase (d-gai) was an efficient host for the production of d-tagatose, a functional sweetener. the d-tagatose production at 500 g/l d-galactose by the host was 1.4-fold higher than that by escherichia coli expressing d-gai. the d-tagatose-producing activity of permeabilized c. glutamicum (pcg) cells treated with 1% (w/v) triton x-100 was 2.1-fold higher than that of ... | 2016 | 27734668 |
| development of a genetically engineered escherichia coli strain for plasmid transformation in corynebacterium glutamicum. | gene disruption and replacement in corynebacterium glutamicum is dependent upon a high transformation efficiency. the cglir-cgiir restriction system is a major barrier to introduction of foreign dna into corynebacterium glutamicum cells. to improve the transformation efficiency of c. glutamicum, the cglim gene encoding methyltransferase in the cglir-cgliir-cglim restriction-modification system of c. glutamicum atcc 13032 was chromosomally integrated and expressed in escherichia coli, resulting i ... | 2016 | 27793586 |
| a new metabolic route for the production of gamma-aminobutyric acid by corynebacterium glutamicum from glucose. | gamma-aminobutyric acid (gaba), a non-protein amino acid widespread in nature, is a component of pharmaceuticals, foods, and the biodegradable plastic polyamide 4. corynebacterium glutamicum shows great potential for the production of gaba from glucose. gaba added to the growth medium hardly affected growth of c. glutamicum, since a half-inhibitory concentration of 1.1 m gaba was determined. as alternative to gaba production by glutamate decarboxylation, a new route for the production of gaba vi ... | 2016 | 27289384 |
| overexpression of methionine adenosyltransferase in corynebacterium glutamicum for production of s-adenosyl-l-methionine. | two genes encoding methionine adenosyltransferase, sam2 from saccharomyces cerevisiae and metk from corynebacterium glutamicum, were individually cloned into pdxw-8, the shuttle vector between escherichia coli and c. glutamicum, and overexpressed in e. coli dh5α and c. glutamicum atcc13032. in dh5α, both genes were overexpressed and their protein products showed the activity of methionine adenosyltransferase. in atcc13032, metk was overexpressed, its product metk showed the enzyme activity and c ... | 2016 | 26238196 |
| stereospecificity of corynebacterium glutamicum 2,3-butanediol dehydrogenase and implications for the stereochemical purity of bioproduced 2,3-butanediol. | the stereochemistry of 2,3-butanediol (2,3-bd) synthesis in microbial fermentations is important for many applications. in this work, we showed that corynebacterium glutamicum endowed with the lactococcus lactis genes encoding α-acetolactate synthase and decarboxylase activities produced meso-2,3-bd as the major end product, meaning that (r)-acetoin is a substrate for endogenous 2,3-butanediol dehydrogenase (bdh) activity. this is curious in view of the reported absolute stereospecificity of c. ... | 2016 | 27687994 |
| the topology of the l-arginine exporter argo conforms to an nin-cout configuration in escherichia coli: requirement for the cytoplasmic n-terminal domain, functional helical interactions, and an aspartate pair for argo function. | argo and lyse are members of the lyse family of exporter proteins and ordinarily mediate the export of l-arginine (arg) in escherichia coli and l-lysine (lys) and arg in corynebacterium glutamicum, respectively. under certain conditions, argo also mediates lys export. to delineate the arrangement of argo in the cytoplasmic membrane of e. coli, we have employed a combination of cysteine accessibility in situ, alkaline phosphatase fusion reporters, and protein modeling to arrive at a topological m ... | 2016 | 27645388 |
| time-resolved, single-cell analysis of induced and programmed cell death via non-invasive propidium iodide and counterstain perfusion. | conventional propidium iodide (pi) staining requires the execution of multiple steps prior to analysis, potentially affecting assay results as well as cell vitality. in this study, this multistep analysis method has been transformed into a single-step, non-toxic, real-time method via live-cell imaging during perfusion with 0.1 μm pi inside a microfluidic cultivation device. dynamic pi staining was an effective live/dead analytical tool and demonstrated consistent results for single-cell death in ... | 2016 | 27580964 |
| high-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in escherichia coli. | succinate has been identified by the u.s. department of energy as one of the top 12 building block chemicals, which can be used as a specialty chemical in the agricultural, food, and pharmaceutical industries. escherichia coli are now one of the most important succinate producing candidates. however, the stoichiometric maximum succinate yield under anaerobic conditions through the reductive branch of the tca cycle is restricted by nadh supply in e. coli. | 2016 | 27520031 |
| the flexible feedstock concept in industrial biotechnology: metabolic engineering of escherichia coli, corynebacterium glutamicum, pseudomonas, bacillus and yeast strains for access to alternative carbon sources. | most biotechnological processes are based on glucose that is either present in molasses or generated from starch by enzymatic hydrolysis. at the very high, million-ton scale production volumes, for instance for fermentative production of the biofuel ethanol or of commodity chemicals such as organic acids and amino acids, competing uses of carbon sources e.g. in human and animal nutrition have to be taken into account. thus, the biotechnological production hosts e. coli, c. glutamicum, pseudomona ... | 2016 | 27491712 |
| d-allulose production from d-fructose by permeabilized recombinant cells of corynebacterium glutamicum cells expressing d-allulose 3-epimerase flavonifractor plautii. | a d-allulose 3-epimerase from flavonifractor plautii was cloned and expressed in escherichia coli and corynebacterium glutamicum. the maximum activity of the enzyme purified from recombinant e. coli cells was observed at ph 7.0, 65°c, and 1 mm co2+ with a half-life of 40 min at 65°c, km of 162 mm, and kcat of 25280 1/s. for increased d-allulose production, recombinant c. glutamicum cells were permeabilized via combined treatments with 20 mg/l penicillin and 10% (v/v) toluene. under optimized con ... | 2016 | 27467527 |
| progress in the microbial production of s-adenosyl-l-methionine. | s-adenosyl-l-methionine (sam), which exists in all living organisms, serves as an activated group donor in a range of metabolic reactions, including trans-methylation, trans-sulfuration and trans-propylamine. compared with its chemical synthesis and enzyme catalysis production, the microbial production of sam is feasible for industrial applications. the current clinical demand for sam is constantly increasing. therefore, vast interest exists in engineering the sam metabolism in cells for increas ... | 2016 | 27465853 |
| engineering a glycerol utilization pathway in corynebacterium glutamicum for succinate production under o2 deprivation. | to explore the glycerol utilization pathway in corynebacterium glutamicum for succinate production under o2 deprivation. | 2016 | 27395064 |
| enhanced succinate production from glycerol by engineered escherichia coli strains. | in this study, an engineered strain escherichia coli mlb (ldha(-)pflb(-)) was constructed for production of succinate from glycerol. the succinate yield was 0.37mol/mol in anaerobic culture, however, the growth and glycerol consumption rates were very slow, resulting in a low succinate level. two-stage fermentation was performed in flasks, and the succinate yield reached 0.93mol/mol, but the succinate titer was still low. hence, overexpression of malate dehydrogenase, malic enzyme, phosphoenolpy ... | 2016 | 27371794 |
| roles of export genes cgma and lyse for the production of l-arginine and l-citrulline by corynebacterium glutamicum. | l-arginine is a semi-essential amino acid with application in cosmetic, pharmaceutical, and food industries. metabolic engineering strategies have been applied for overproduction of l-arginine by corynebacterium glutamicum. lyse was the only known l-arginine exporter of this bacterium. however, an l-arginine-producing strain carrying a deletion of lyse still accumulated about 10 mm l-arginine in the growth medium. overexpression of the putative putrescine and cadaverine export permease gene cgma ... | 2016 | 27350619 |
| three-steps in one-pot: whole-cell biocatalytic synthesis of enantiopure (+)- and (-)-pinoresinol via kinetic resolution. | pinoresinol is a high-value plant-derived lignan with multiple health supporting effects. enantiomerically pure pinoresinol can be isolated from natural sources, but with low efficiency. most chemical and biocatalytic approaches that have been described for the synthesis of pinoresinol furnish the racemic mixture. in this study we devised a three-step biocatalytic cascade for the production of enantiomerically pure pinoresinol from the cheap compound eugenol. two consecutive oxidations of eugeno ... | 2016 | 27160378 |
| updates on industrial production of amino acids using corynebacterium glutamicum. | l-amino acids find various applications in biotechnology. l-glutamic acid and its salts are used as flavor enhancers. other l-amino acids are used as food or feed additives, in parenteral nutrition or as building blocks for the chemical and pharmaceutical industries. l-amino acids are synthesized from precursors of central carbon metabolism. based on the knowledge of the biochemical pathways microbial fermentation processes of food, feed and pharma amino acids have been developed. production str ... | 2016 | 27116971 |
| properties of cassava starch modified by amylomaltase from corynebacterium glutamicum. | amylomaltase (α-1,4-glucanotransferase, am; ec 2.4.1.25) from corynebacterium glutamicum expressed in escherichia coli was used to prepare the enzyme-modified cassava starch for food application. about 5% to 15% (w/v) of cassava starch slurries were incubated with 1, 3, or 5 units of amylomaltase/g starch. apparent amylose, amylopectin chain length distribution, thermal properties, freeze-thaw stability, thermo-reversibility, and gel strength of the obtained modified starches were measured. the ... | 2016 | 27105125 |