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demonstration by confocal laserscanning microscopy of invasive potential with bacteroides fragilis.the confocal laserscanning microscopy (clsm) system provides a stereoscopic view of an object. by this system the penetration of b. fragilis into hela cells was observed. the intensity of contact is highlighted with time. the clsm system consolidates the recently described fluorescence technique to test invasive potential. this work purports that certain gram-negative anaerobes should be considered for invasiveness.19912067416
in vivo metal substitution in bacteroides fragilis superoxide dismutase.bacteroides fragilis, an obligate anaerobe, synthesizes an azide-inhibitable iron-containing superoxide dismutase when grown in complex medium. cells grown anaerobically in complex media containing desferrioxamine (desferal, ciba-geigy) and graded concentrations of mn synthesize the azide-resistant manganese-containing sod. the fraction of mnsod activity in dialyzed cell extracts increased progressively as the mn concentration in the medium increased. the fraction of mnsod activity also increase ...19912071036
early events after intra-abdominal infection with bacteroides fragilis and escherichia coli.growth of bacteroides fragilis and escherichia coli was monitored during early stages of single (mono-) and mixed intra-abdominal infection in a rat fibrin clot model. when b. fragilis and e. coli were together involved in the infection, b. fragilis numbers increased about 6 h after an initial decline. this increase was not found with b. fragilis mono-infections. the numbers of e. coli increased rapidly in both mono- and mixed infections and stayed high for several days, but only mixed infection ...19912072375
autologous fibrin gel: bactericidal properties in contaminated hepatic injury.fibrin glue is an effective hemostatic agent in a variety of clinical situations; its utility is limited by potential transmission of viral infection. we studied the bactericidal properties of fibrin gel (fg) in a murine contaminated hepatic injury model and in vitro by agar plate culture method. intra-abdominal abscess formation and adhesion rate were assessed following controlled liver injury in association with abdominal contamination with 10(7) bacteroides fragilis and hepatorrhaphy (h, n = ...19912072441
characterization of beta-lactamases from non-bacteroides fragilis group bacteroides spp. belonging to seven species and their role in beta-lactam resistance.twelve beta-lactamase-positive non-bacteroides fragilis group bacteroides spp. belonging to seven different species were examined by mic determination and enzyme characterization. mics of most beta-lactams except cefoxitin, cefotetan, imipenem, and meropenem were relatively high or very high. all enzymes hydrolyzed cephaloridine (vmax, 100%; km, 12 to 70 microm), cephalothin (vmax, 25 to 826%; km, 8 to 143 microm), cefamandole (vmax, 13 to 158%; km, 17 to 170 microm), and cefuroxime (hydrolysis ...19902073107
statistical analysis of the effects of trial, reader, and replicates on mic determination for cefoxitin.a pilot study was designed to estimate the variance components in the determination of the mic of cefoxitin for isolates of the bacteroides fragilis group. twenty different organisms were tested, and replicate, trial, and reader variabilities were examined. when the total-variance component was used, if the true mic was 16 micrograms/ml, then the chance that the observed mic was between 8 and 32 micrograms/ml, inclusive, was 95%. for all analyses, the isolate (p = 0.0001) and reader (p less than ...19902073115
the in-vitro activity of cefodizime: a review.for enterobacteriaceae, mic50s and mic90s of cefodizime (mg/l), respectively, were as follows, for naturally non-beta-lactamase-producing species: escherichia coli 0.12 and 0.5, salmonella spp. and shigella spp. 0.25 and 0.5, proteus mirabilis 0.016 and 0.03; for chromosomal penicillinase-producing species. klebsiella spp. 0.25 and 64, and for chromosomal cephalosporinase-producing species. enterobacter cloacae 1 and 64, citrobacter freundii 1 and 128, serratia marcescens 2 and 8: indole-positiv ...19902074248
[recurrent thromboembolism disclosing protein c deficiency. apropos of a case with familial investigation].every thromboembolic manifestation, especially in young subjects, calls for an aetiological study in which haemostasis is evaluated primarily with assays of physiological coagulation inhibitors: protein c, protein s and antithrombin iii. protein c deficiency is found in 6 to 7% of thromboembolic manifestations. we report the case of a 21-year old man who had phlebitis followed by pulmonary embolism without facilitating factors. protein c level was 50% of normal value (0.50 iu/ml). the patient re ...19902075365
differences in the in vitro inhibitory and bactericidal activity of ceftizoxime, cefoxitin, cefotetan, and penicillin g against bacteroides fragilis group isolates. comparison of time-kill kinetic studies with mic values.nineteen strains of the bacteroides fragilis group were used to determine the bactericidal activity of ceftizoxime, cefoxitin, cefotetan, and penicillin g with time-kill kinetics studies. each antimicrobial agent was tested at subinhibitory (1/2 x mic), inhibitory (1 x mic), and suprainhibitory (4 x mic) concentrations. penicillin g exhibited virtually no sustained bactericidal activity at any of the antimicrobial concentrations tested. at subinhibitory concentrations, ceftizoxime was considerab ...19902076592
in vitro activity of sparfloxacin (at-4140, ci-978, pd 131501), a new quinolone antimicrobial agent.in vitro activity of sparfloxacin (at-4140, ci-978, pd 131501) against clinical bacterial isolates was compared with those of ciprofloxacin and vancomycin or imipenem. sparfloxacin was more active or equal to ciprofloxacin against most gram-positive species and against bacteroides fragilis, and it inhibited virtually all enterobacteriaceae at 1.0 micrograms/ml or less.19902076597
pharmacokinetics of imipenem in patients undergoing major colon surgery.ten patients about to undergo a colorectal operation lasting an average of three hours received 500 mg each of imipenem and cilastatin i.v. preoperatively. during the operation blood and tissue samples were taken in order to determine the serum kinetics of the substances as well as the levels of imipenem in the cutis, subcutis, fascia, muscle, parietal peritoneum and colon. the imipenem concentrations were measured by hplc. the mean peak serum concentration was 26 mg/l, the mean half-life 55 min ...19902076910
an experimental study of lymphatic vessel autotransplantation in the dog.to provide greater insight into microsurgical treatment of lymphedema, 7 mongrel dogs underwent autotransplantation of a lymphatic collector into the contralateral lower limb. two months later, lymphatic vessels were studied using patent blue dye, light microscopy, and histomorphometry. patency of the transplanted lymphatics was uniformly observed although there was a notable increase in the width of the intima and a corresponding reduction in the size of the lymphatic lumen. of note, there was ...19902077300
animal model for chronic-abscess-forming peritonitis: histology and microbiology.clinical experience gives evidence of the increasing importance of chronic-abscess-forming peritonitis with a general physical impairment in spite of a well-controlled abdominal situation. the number of unsolved problems led us to develop a standardized and reproducible experimental animal model, using wistar rats. we induced peritonitis by intraperitoneal injection of a mixture containing a capsulated strain of bacteroides fragilis and dev agar. the histologic changes in the parietal and viscer ...19902079095
in-vitro activity of sparfloxacin, a new quinolone antimicrobial agent.the in-vitro activity of sparfloxacin (at-4140), a new difluorinated quinolone, was compared with those of ciprofloxacin, temafloxacin and selected members of other groups of antimicrobial agents, against 651 recent distinct clinical isolates and strains with known mechanisms of resistance. three strains of chlamydia trachomatis were also studied. the mics for 90% of the enterobacteriaceae were between 0.06 and 1 mg/l; for pseudomonas aeruginosa the mic90 was 2 mg/l. sparfloxacin was 16-fold mor ...19902079449
detection of non-volatile organic acids by head-space gas chromatography. 19902084133
clinical evaluation of the imipenem/cilastatin combination in the therapy of severe infections in patients with malignant diseases.thirty hospitalized patients (23 men and 7 women), aged 42 to 78 years, with impaired host defences for malignant underlying diseases and affected by life-threatening infections, were treated with the imipenem/cilastatin combination at a dosage ranging between 2 and 4 g daily i.v. at 8 or 12 h intervals. the average length of therapy was 9.6 +/- 2.09 days. the isolated organisms were as follows: pseudomonas aeruginosa [12], escherichia coli [10], klebsiella pneumoniae [5], proteus mirabilis [3], ...19902086164
a collaborative study of french laboratories to assess any evolution of antibiotic resistance within the bacteroides fragilis group.antibiotic susceptibility testing of anaerobes by a same methodology allows the authors to draw up suggestions about the evolution of antibiotic resistance within the b. fragilis group. cefoxitin resistance rates were stable until 1985 and were slowly increasing later. until 1985 piperacillin was able to inhibit all tested strains. in 1987 the two groups noticed an increasing resistance to piperacillin (4 to 9%). during the 1970's clindamycin resistance was a minor event (less than 1%) then the ...19902086755
[susceptibilities of clinical bacterial isolates to antimicrobial agents. a study mainly focused on imipenem. research group for testing imipenem susceptibility on clinical isolates].we investigated susceptibilities of clinical bacterial isolates to imipenem (ipm) and other antimicrobial agents at 459 hospital laboratories throughout japan from september to december of 1988. in this study, identification and susceptibility testing were performed at each hospital laboratory and the tests were carried out according to the 1-dilution or 3-dilution disc technique in which susceptibilities are classified into 4 grades: , ++, + and -. ipm had significantly high activity against st ...19902086814
a simple technique useful for clinical laboratory testing for invasive potential applied to bacteroides fragilis.a simple technique using fluorescent microscopy examines the association between bacteroides fragilis and different types of tissue cells (epithelial, fibroblast, osteoblast). through this stepwise intracellular staining and extracellular quenching some latent signs of invasive quality may be exhibited. the bacteria are incubated with monolayers of tissue cells, and the reactions immediately visualized after staining with acridine orange and masking by crystal violet. intact bacterial rods exhib ...19902087201
[soft-tissue necrosis of the vulva and perineum]. 19902090215
combination of ofloxacin and other antimicrobial agents.we evaluated the combination of ofloxacin with piperacillin, gentamicin, vancomycin, rifampin, clindamycin, and metronidazole by the checkerboard agar dilution method against 165 isolates which included escherichia coli, klebsiella pneumoniae, enterobacter cloacae, serratia marcescens, pseudomonas aeruginosa, providencia stuartii, acinetobacter, staphylococcus aureus, bacteroides fragilis, and citrobacter perfringens. synergy of piperacillin-ofloxacin was demonstrated for 50% and 40% respectivel ...19902093103
[phosphorylation of tryptophanyl-trna-synthetase by casein kinase type ii].incubation of tryptophanyl-trna synthetase from bovine pancrease with [gamma-32p]atp of [gamma-32p]gtp and casein kinase ii from rabbit liver leads to the incorporation of labeled phosphate into serine residues of synthetase polypeptide. the maximal level of 32p incorporation into synthetase polypeptide (mr = 60 kda) 0.15 moles of 32p per 1 mole of polypeptide was observed. electrophoretic analysis according to o'farrell showed that kinase phosphorylates exclusively the most acidic polypeptides ...19902094810
plasmid transformation of bacteroides spp. by electroporation.transformation of bacteroides spp. with a variety of plasmid dnas was accomplished using electroporation. the standard transformation assay system used to deduce the optimal electroporation parameters employed a 50-to 100-fold concentrated cell suspension of mid-logarithmic phase bacteroides fragilis strain 638 and the 5.4-kb clindamycin resistance (ccr) vector, pbi191. a variety of electroporation buffers were used successfully in transformation experiments but of these, 1 mm mgcl2 in 10% glyce ...19902096397
plasmid analysis of clinical isolates of bacteroides fragilis group strains.cryptic plasmids were isolated from 26 of 52 bacteroides fragilis group strains derived from severe infections. four strains harboured two plasmids, one three plasmids and one five plasmids with different molecular weights. the same molecular weight plasmid (3.7 md) was isolated from 17 of the 26 plasmid-containing b. fragilis group strains. no correlation was found between plasmid-harbouring and resistance against ten antibiotics and different heavy metal ions. no curing of the strains from the ...19902099083
immune response to endotoxin isolated from bacteroides fragilis in the pregnant guinea pig.the humoral immune response to endotoxin isolated from bacteroides fragilis was analyzed in the pregnant guinea pig by means of passive hemagglutination, passive hemolysis, a modified coombs test, and by crossed immunoelectrophoresis. pregnant animals were immunized with endotoxin on day 30 of gestation, and antibodies were determined on day 61 in maternal and fetal sera, and in amniotic fluid. the igg and igm responses in maternal sera were of the same magnitude as in sera of nonpregnant animal ...19902099349
assessment of abdominal fat deposition in female cynomolgus monkeys.computed tomography (ct) was used to assess abdominal fat distribution in adult, female cynomolgus macaques (macaca fascicularis). the technique used was similar to that applied in human beings and allowed intra-abdominal, subcutaneous and total abdominal fat to be quantitated in 1 cm thick sections. correlations between single scans and the average of scans at several levels ranged from r = 0.96 to r = 1.00, indicating that a single scan is representative of abdominal fat distribution. signific ...19912045214
efficacy and safety of recombinant human erythropoietin to prevent the anaemias of prematurity. european randomized multicenter trial. 19912040194
[spleen abscess].3 cases of splenic abscess (sa), all of them with similar clinical findings; abdominal pain, liver and splenic enlargement and leukocytosis, are presented. the diagnosis was suspected by abdominal ultrasound and confirmed by ctr. we focused the interest on the etiopathogenesis, diagnosis and the rareness of the bacteria isolated: salmonella group d (two cases) and bacteroides fragilis.19902103770
isolation and identification of strains of bacteroides fragilis group from the digestive tract of callithrix penicillata marmosets.thirty-five strains of the bacteroides fragilis group were isolated from oral and intestinal samples from 5 wild caught, captive callithrix penicillata. nine oral strains of bacteroides fragilis (7) and bacteroides distasonis (2), and 26 intestinal strains of bacteroides fragilis (14) and bacteroides distasonis (12) were identified.19902106054
interferon gamma and tumor necrosis factor alpha. use in gram-negative infection after shock.shock increases the propensity to develop infection after injury or operation. this study evaluated the effect of cefoxitin, interferon gamma (inf-gamma), and tumor necrosis factor alpha (tnf-alpha) on the development of a polymicrobial soft-tissue infection. after sham operation or hemorrhagic shock and resuscitation, sprague-dawley rats were inoculated with 1 x 10(8) escherichia coli and 1 x 10(9) bacteroides fragilis in a 5% fecal suspension. animals received either no treatment, cefoxitin, r ...19902108652
sialidase (neuraminidase) activity among gram-negative anaerobic and capnophilic bacteria.a filter paper spot test with 2'-(4-methylumbelliferyl)-alpha-d-n-acetylneuraminic acid as a substrate was used to study the prevalence of sialidase activity among gram-negative anaerobic and capnophilic bacteria. a total of 567 isolates representing four genera of obligate anaerobes and four genera of capnophilic organisms was tested. sialidase activity was detected in 94% of 66 isolates from the bacteroides fragilis group, 98% of 66 b. bivius isolates, and all isolates of the following species ...19902108991
sequencing the gene for an imipenem-cefoxitin-hydrolyzing enzyme (cfia) from bacteroides fragilis tal2480 reveals strong similarity between cfia and bacillus cereus beta-lactamase ii.using a newly constructed bacteroides fragilis-escherichia coli cloning shuttle vector, pjst61, we have cloned the cefoxitin (fox)-imipenem (imp) resistance determinant from b. fragilis tal2480. fox-imp resistance in this strain results from the production of a periplasmic, zn2(+)-containing beta-lactamase which hydrolyzes carbapenems and cephamycins and whose activity is resistant to clavulanic acid but sensitive to zn2(+)-binding reagents, including edta. the pjst61 vector permits efficient li ...19902110145
complete nucleotide sequences of seven eubacterial genes coding for the elongation factor tu: functional, structural and phylogenetic evaluations.the nucleotide sequences of cloned genes coding for the elongation factor tu of seven eubacteria have been determined. these genes were from anacystis nidulans, bacillus subtilis, bacteroides fragilis, "deinonema" spec., pseudomonas cepacia, shewanella putrefaciens and streptococcus oralis. the primary structures of the genes were compared to the available sequences of prokaryotic elongation factors tu and eukaryotic elongation factors 1 alpha. a conservation profile was determined for homologou ...19902110445
biological characterization of a new radioactive labeling reagent for bacterial penicillin-binding proteins.radiolabeled penicillin g is widely used as the imaging agent in penicillin-binding protein (pbp) assays. the disadvantages of most forms of labeled penicillin g are instability on storage and the long exposure times usually required for autoradiography or fluorography of electrophoretic gels. we investigated the utility of radioiodinated penicillin v as an alternative reagent. radioiodination of p-(trimethylstannyl)penicillin v with [125i]na, using a modification of the chloramine-t method, is ...19902113792
beta-lactamase of lysobacter enzymogenes: induction, purification and characterization.lysobacter enzymogenes produces an inducible beta-lactamase and induction with 100 micrograms ampicillin ml-1 resulted in an increase of more than 100-fold in enzyme activity. various other beta-lactam antibiotics also served as effective inducers. the enzyme was obtained from cells by osmotic shocking to release periplasmic components and it was purified primarily by ion-exchange chromatography and page. the beta-lactamase consists of one polypeptide with a molecular mass of about 28 kda and an ...19902118167
occlusive dressings. does dressing type influence the growth of common bacterial pathogens?we studied the effect of different occlusive dressings and of air exposure on the growth of four pathogenic bacteria in wounds. partial-thickness wounds on domestic pigs were inoculated with staphylococcus aureus, clostridium perfringens, bacteroides fragilis, or pseudomonas aeruginosa. each wound was covered with three dressings (duoderm, opsite, or vigilon), or left exposed to air. groups of wounds were sampled at 24, 48, and 72 hours. staphylococcus aureus reached high levels beneath all of t ...19902119166
intra-abdominal abscess in children: a 13-year experience.the microbiology of intra-abdominal abscess in children is similar to that of adults--a mixture of aerobes and anaerobes. in abscess specimens taken from 36 children, predominant pathogens were bacteroides fragilis group species, peptostreptococcus species, and escherichia coli.19902120268
effects of differences in media and methods on mic values for anaerobes.in vitro susceptibility of bacteroides fragilis isolates to cefoxitin at the breakpoint of 32 micrograms/ml varied little when tested by eight different methods. we found that broth disk elution results were difficult to read and interpret and did not correlate with other methods.19902120269
gangrenous and/or perforated appendix: clinical outcome and in vitro susceptibility testing.the data from this study indicate that cefoxitin was effective and generally well tolerated in the management of gangrenous and/or perforated appendicitis. no strong correlation was identified between in vitro susceptibility testing results and clinical outcome.19902120270
cloning and sequencing of the class b beta-lactamase gene (ccra) from bacteroides fragilis tal3636.bacteroides fragilis tal3636 produces a class b, zn2(+)-requiring beta-lactamase. the gene, ccra, was cloned and expressed in escherichia coli. the gene was sequenced and shown to share greater than 33% identity with the metalloenzyme from bacillus cereus 569/h.19902121094
in vitro activity of dirithromycin (ly 237216) compared with activities of other macrolide antibiotics.dirithromycin inhibited streptococcus pyogenes, streptococcus pneumoniae, and other hemolytic streptococci at concentrations of less than or equal to 0.03 to 0.12 micrograms/ml, with 90% inhibition at 0.12 micrograms/ml, which is comparable to results using erythromycin. group a streptococci, listeriae, and enterococci resistant to erythromycin were resistant to dirithromycin. erythromycin-susceptible staphylococci were inhibited by 0.5 micrograms/ml, but for erythromycin-resistant isolates mics ...19902126695
suppression of blastogenic transformation of lymphocytes by bacteroides fragilis in vitro and in vivo.bacteroides species and enterobacteriaceae are known to cause synergistic infections. however, the mechanisms behind this synergy are not completely understood. several authors have shown that bacteroides species may inhibit the phagocytosis of enterobacteriaceae by polymorphonuclear leukocytes as well as by macrophages. with the present study we have addressed the question of whether bacteroides fragilis (bf) is also capable of suppressing specific immune functions. when incubated together with ...19902128603
radio-immunoassay for formyl methionyl leucyl phenylalanine. i. development and application to assessment of chemotactic peptide production by enteric bacteria.bacterial chemotactic peptides are low molecular weight peptides which stimulate a wide range of neutrophil functions following binding to specific leucocyte receptors. formyl methionyl leucyl phenylalanine (fmlp) is the major chemotactic peptide in escherichia coli culture supernatants. this paper reports the development and validation of a radio-immunoassay (ria) for fmlp and its application to the analysis of formyl peptide production by enteric bacteria in vitro. the assay was moderately sen ...19902129421
rheological properties of the extracellular polysaccharide from agrobacterium sp.s-1231.a strain of agrobacterium sp. s-1231 was isolated from one of the soil samples taken from beijing area. in 4 days' cultivation it was found to produce extracellular polysaccharide and about 24 grams per liter of sugar-containing substrate. at the same concentrations, the polymer produced higher viscosity than that of xanthan gum (kelzan). the viscosities of 0.2%, 0.5% and 1.0% solutions of this polymer were found to be 840, 5200 and 15000 cp respectively, measured with a brookfield lvf viscosime ...19902129792
quantitative study of apoptosis in normal rat gastroduodenal mucosa.the occurrence of apoptosis in the normal gastrointestinal mucosa has been given little consideration until now, although the phenomenon may be of interest in the light of recent hypotheses about its role in physiological cell renewal. in the present study, a quantitative evaluation conducted on normal gastric and duodenal mucosa of young rats has shown that apoptosis is a rare but constant phenomenon: 1.4 +/- 1.1 (mean +/- 1 s.d.) apoptotic bodies were observed within the surface epithelium of ...19902129807
antibacterial activity of combined cefotaxime and desacetyl-cefotaxime against aerobic and anaerobic gram-negative bacilli.synergy between cefotaxime and desacetyl-cefotaxime was evaluated against 12 strains (9 enterobacteriaceae and 3 bacteroides fragilis) by the chequerboard technique. a 1:1 combination of cefotaxime and desacetyl-cefotaxime was synergistic against two-thirds of the 12 strains tested. the in vitro activity of the combination was compared with that of four other beta-lactam antibiotics against 96 recent clinical isolates: 78 enterobacteriaceae, 8 haemophilus influenzae, 10 b. fragilis. the mics of ...19902130005
[gangrenous and perforating appendicitis in a provincial hospital: a 48-month retrospective study. clinical and microbiological aspects, course and postoperative morbidity].the appendectomy for gangrenous or perforated appendicitis is made more serious by a very high frequency of infection. the antibiotic prophylaxis should be able to reduce the frequency of such complications. we have considered retrospectively the patients who underwent appendectomy for gangrenous and perforated appendicitis in the period from june 1st, 1986 to may 31st 1990 in the ivrea-castellamonte hospital (province of turin), focusing our attention on certain microbiological and clinical var ...19902133325
[community- and hospital-acquired bacteremia: a retrospective study in a regional hospital. iii. microbiological aspects].a retrospective study was made of all blood cultures performed over a 40-month period at the ivrea-castellamonte hospital (turin, italy). a total of 4386 vials from 619 patients were examined. there were 619 positive vials (14.1%) from 131 patients (21.2%) corresponding to 145 bacteremia episodes, including 129 monomicrobial (89%) and 16 polymicrobial (11%). ten patients (1.6%) had more than one episode. there were 73 polluted vials (1.7%). a total of 165 microorganism were isolated: gram-positi ...19902133330
[studies on suppurative lesion and bone distribution of peroral antimicrobial agents (lapc and tflx) to experimental infected rabbits].the models of infection in the rabbits mandible using streptococcus milleri and bacteroides fragilis by the method of satoh-heimdahl were prepared. a penicillic peroral antimicrobial agent, lenamipicillin (lapc), and pyridone carboxylic acid peroral antimicrobial agent, tosufloxacin tosilate (tflx), were administered to infected and healthy groups. drug concentrations in the serum, pus, mandible, maxilla, humerus, sternum, costa, ilium, femur, tibia, liver, and kidney were determined by a biolog ...19902134803
penetration of clindamycin, cefoxitin, and metronidazole into pelvic peritoneal fluid of women undergoing diagnostic laparoscopy.a single dose of clindamycin, cefoxitin, or metronidazole was administered to each of 30 women. the mean concentration of cefoxitin in pelvic fluid at 1 h exceeded those of the other two drugs (p less than 0.007). cefoxitin concentrations were inferior to those of the other drugs when compared with the published mic for 90% of bacteroides fragilis strains.19902139315
in vitro activity of teicoplanin, vancomycin, a16686, clindamycin, erythromycin and fusidic acid against anaerobic bacteria.the in vitro activity of teicoplanin and a16686, two new glycopeptide antibiotics was determined against 196 isolates of anaerobic bacteria. the activity of teicoplanin and a16686, in comparison with that of vancomycin, clindamycin, erythromycin and fusidic acid was 2 to 16 times higher against the gram positive anaerobes, namely, propionibacterium acnes, clostridium perfringens, clostridium difficile, clostridium species, peptococcus species and peptostreptococcus species. however, bacteroides ...19902139737
[in vitro activity of imipenem against hospital bacteria].imipenem proved highly active against enterobacteriaceae: the different bacterial groups exhibited similar mode mics (0.12 to 0.25 micrograms/ml), except for serratia (0.25-0.5 micrograms/ml), proteus mirabilis (0.5 micrograms/ml), indole-positive proteus (2 micrograms/ml) and providencia (1 mu/ml). the mics of cefotaxime-resistant strains (cephalosporinase hyperproducing or very broad spectrum betalactamase producing) were within the susceptibility range. imipenem also exhibited satisfactory ac ...19902139936
[in vitro antibacterial activity of clarithromycin, a new macrolide antibiotic, and regression curve].this study was set up to establish the regression curve for clarithromycin inhibition zone diameters (disks 15 micrograms) and mic to create a strain distribution plot, in order to allow accurate interpretation of the disk diffusion method for testing susceptibility to clarithromycin. 430 bacterial strains were studied in three university hospital. clarithromycin was active against erythromycin sensitive staphylococcus aureus and coagulase negative staphylococci at concentrations of 0.12 to 0.25 ...19902142274
comparative in-vitro activity of azithromycin and erythromycin against gram-positive cocci, haemophilus influenzae and anaerobes.the in-vitro activities of azithromycin and erythromycin were compared against 689 clinical isolates, including gram-positive cocci, haemophilus influenzae, and anaerobes. of the 100 methicillin-susceptible isolates of staphylococcus aureus tested, 77% were susceptible to 1 mg/l azithromycin and 0.5 mg/l erythromycin, whereas 22% were resistant to 32 mg/l of both compounds. all methicillin-resistant s. aureus isolates were highly resistant to both macrolides (mic greater than 64 mg/l). coagulase ...19902154433
correlation of the extravascular pharmacokinetics of azithromycin with in-vivo efficacy in models of localized infection.infection models were used to clarify the roles of serum and extravascular concentrations in the in-vivo efficacy observed with azithromycin. in-vivo experiments were designed to give serum concentrations well below the mic and tissue levels generally above the mic at time of challenge and during the course of infection. the efficacy of azithromycin against a salmonella enteritidis oral challenge (a tissue-associated infection model) in mice correlated directly with azithromycin liver levels, bu ...19902154439
cloning and expression of the bacteroides fragilis tal2480 neuraminidase gene, nanh, in escherichia coli.we have cloned the bacteroides fragilis tal2480 neuraminidase (nanase) structural gene, nanh, in escherichia coli. this was accomplished by using the cloning shuttle vector pjst61 and a partial sau3a library of tal2480 chromosomal inserts created in e. coli. the library was mobilized into the nanase-deficient b. fragilis tm4000 derivative tc2. nanase-producing colonies were enriched by taking advantage of the inability of tc2, but not the wild-type of nanase+ revertant, to grow in vitro in fluid ...19902158974
turbidimetric and microscopic analysis of bacteroides fragilis exposed to tazobactam and piperacillin alone and in combination.tazobactam, a non-amino penicillanic acid sulfone, is a new beta-lactamase inhibitor and acts synergistically with piperacillin against clinical isolates of beta-lactamase-producing bacteroides fragilis. the effectiveness of this inhibitor has been demonstrated by reduction of piperacillin mic values and growth curves in two combinations (ratio 8:1 and 4:1). tazobactam has an intrinsic activity against b. fragilis (mic = 4-8 micrograms/ml). phase contrast microscopy of treated cells showed cell- ...19902159865
disparate mechanisms of induction of procoagulant activity by live and inactivated bacteria and viruses.this study describes the dose response, time course, and lymphocyte requirements of procoagulant activity (pca) induction following stimulation of thioglycolate-elicited balb/c peritoneal macrophages with live and inactivated bacteria (bacteroides fragilis, escherichia coli, and staphylococcus aureus) and murine hepatitis virus type 3 (mhv-3). induction of pca by mhv-3 was significantly more rapid and the maximal pca achieved was significantly greater than by the three bacterial species studied. ...19902160434
in vitro activity of daptomycin-metronidazole combinations against mixed bacterial cultures: reduced activity of metronidazole against bacteroides species in the presence of enterococcus faecalis.the in vitro activity of daptomycin-metronidazole combinations against mixed cultures of gram-positive facultative cocci and strains of the bacteroides fragilis group was investigated. metronidazole did not influence the high activity of daptomycin against strains of staphylococcus aureus, staphylococcus epidermidis and enterococcus faecalis in the absence or presence of the co-cultured bacteroides strains. in contrast, the enterococcus faecalis isolates protected the co-cultured bacteroides str ...19902161762
expression and regulation of a bacteroides fragilis sucrose utilization system cloned in escherichia coli.a bacteroides fragilis strain isolated from human feces was the source of chromosomal dna in the construction of plasmid pbs100. the cloned 6-kilobase insert of plasmid pbs100 conferred a sucrose positivity phenotype on transformed cells of escherichia coli ja221. e. coli ja221(pbs100) cells were able to utilize sucrose as the sole source of carbon because of the presence of sucrase enzyme and sucrose uptake activities. sucrase activity was inducible in b. fragilis but constitutive in e. coli ja ...19902166474
synthesis and antiviral activity of 3'-deoxy-3'-c-hydroxymethyl nucleosides.a series of 3'-branched-chain sugar nucleosides, in particular 3'-deoxy-3'-c-hydroxmethyl nucleosides, have been synthesized and evaluated as antiviral agents. reaction of 1-(2,3-epoxy-5-o-trityl-beta-d-lyxo-pentofuranosyl) derivatives 12 and 13, of uracil and thymine, respectively, with 5,6-dihydro-2-lithio-5-methyl-1,3,5-dithiazine 14 afforded the corresponding 3'-functionalized nucleosides 15 and 16, respectively. replacement of the trityl group with tertbutyldiphenylsilyl allowed high yieldi ...19902167981
[new antitubercular drugs]. 19902168041
ki-1 antigen expression defines a favorable clinical subset of non-b cell non-hodgkin's lymphoma.immunohistochemical and immunophenotypic analyses were performed on 278 cases of karyotypically abnormal non-hodgkin's lymphoma (nhl). excluding cases of lymphoblastic lymphoma or mycosis fungoides, 20 cases showed evidence of non-b cell lineage. t cell lineage was proven by genotypic and immunophenotypic analyses in 15 of the 20 cases; five were of ambiguous lineage. all of the non-b lineage cases were of diffuse histology with a large cell component (dlcl). twelve cases expressed the ki-1 anti ...19902168505
protective effect of inactivated pasteurella haemolytica bacterin challenged in bovine herpesvirus-1 experimentally infected calves.formalin-inactivated and sonicated pasteurella haemolytica bacterins were prepared from 1 h cultures of bacterial pellets in rpmi-1640 medium containing 7% fetal calf serum. the bacterial pellets were obtained from logarithmic phase growth of the organism by centrifugation. the protective effect of the vaccine was evaluated in 43 specific-pathogen-free hereford crossbred calves and yearlings in three experiments. cattle were either single vaccinated or boosted via three routes; intratracheally ( ...19902168606
comparative study of chemical modifiers for the determination of molybdenum in milk by electrothermal atomisation atomic absorption spectrometry.a comparative study of various chemical modifiers for the determination of molybdenum in milk by electrothermal atomisation atomic absorption spectrometry was carried out. methods with nitric acid or barium difluoride as the chemical modifier and in the absence of a chemical modifier were studied by introducing the milk samples directly into the graphite furnace with octyl alcohol. the graphite furnace programme, amount of modifier and the calibration and additions graphs were studied in all ins ...19902168685
import into mitochondria of precursors containing hydrophobic passenger proteins: pretreatment of precursors with urea inhibits import.we have studied the import into isolated yeast mitochondria of three hydrophobic passenger proteins attached to the n-terminal cleavable presequence of mitochondrial atpase subunit 9 from neurospora crassa. one natural precursor (pn9) contained n. crassa subunit 9; two chimaeric precursors, n9l/y8-1 and n9l/y9-2, respectively contained yeast mitochondrial atpase subunits 8 and 9. in the absence of urea, pn9 and n9l/y8-1 are imported efficiently but n9l/y9-2 is not imported. after pretreatment of ...19902168755
role of xanthine-xanthine oxidase systems and polymorphonuclear leukocytes in lipid peroxidation in compound 48/80-induced gastric mucosal injury in rats. 19902173878
antimicrobial spectrum of cefpirome combined with tazobactam against the bacteroides fragilis group.cefpirome, a so-called fourth-generation cephalosporin, was tested alone and in combination with the sulfone beta-lactamase inhibitor, tazobactam, against 63 members of the bacteroides fragilis group. the cefpirome mic50 was only 64 micrograms/ml, but the mic was reduced eightfold with tazobactam (2:1 ratio). the addition of tazobactam to cefpirome or the use of metronidazole as a codrug appear to be alternative choices to enhance the antianaerobic spectrum. over 98% of strains had cefpirome-taz ...19902178078
comparative in vitro activities of a new quinolone, win 57273, and piperacillin plus tazobactam against anaerobic bacteria.the in vitro activities of a new quinolone, win 57273, and the combination of piperacillin and tazobactam, a beta-lactamase inhibitor, were compared with those of cefoxitin, ceftizoxime, chloramphenicol, clindamycin, imipenem, metronidazole, and piperacillin for 123 clinical anaerobic isolates. ceftizoxime and cefoxitin had equivalent activities, while metronidazole was active against gram-negative isolates. in the bacteroides fragilis group, species other than b. fragilis were the most resistan ...19902178340
the role of bacterial vaginosis as a cause of amniotic fluid infection, chorioamnionitis and prematurity--a review.antepartum bacterial vaginosis in pregnancy has been related to premature delivery, the recovery of microorganisms from amniotic fluid of women in premature labor with intact membranes, to histologic chorioamnionitis and to the recovery of microorganisms from the placenta or membranes. microorganisms associated with bacterial vaginosis are commonly recovered from the amniotic fluid and chorioamnion of patients who deliver prematurely. in addition, bacterial vaginosis is associated with maternal ...19902178562
multicenter in vitro comparison of piperacillin and nine other antibacterials against 1,629 clinical isolates.the antibacterial spectrum of activity of piperacillin was compared with that of other antibiotics against isolates of escherichia coli, enterobacter cloacae, haemophilus influenzae, klebsiella pneumoniae, pseudomonas aeruginosa, pseudomonas cepacia, pseudomonas maltophilia, serratia marcescens, enterococcus sp, bacteroides fragilis, bacteroides bivius, and clostridium difficile obtained from laboratories at hospitals in st. louis, in memphis, and in newark, new jersey. of the 1,629 isolates tes ...19902183941
microbiology of empyema in children and adolescents.the microbiology of empyema was studied in 72 children and adolescents whose specimens yielded bacterial growth after inoculation for aerobic and anaerobic bacteria. a total of 93 organisms, 60 aerobic or facultative and 33 anaerobic, were isolated. aerobic bacteria was isolated in 48 (67%) patients, anaerobic bacteria in 17 (24%), and mixed aerobic and anaerobic bacteria in 7 (10%). the predominant aerobic or facultative bacteria were haemophilus influenzae (15 isolates), streptococcus pneumoni ...19902184407
optimum therapy for acute pelvic inflammatory disease.neisseria gonorrhoeae is responsible for about one-third to one-half of cases of acute pelvic inflammatory disease (pid), although there is considerable geographical variation. chlamydia trachomatis is also an important aetiological agent, and is currently isolated 4 times more commonly from the cervix than the gonococcus. however, it is now clear that acute pid is polymicrobial in aetiology. even when n. gonorrhoeae and/or c. trachomatis are isolated from the endocervix, anaerobes such as bacte ...19902190794
bacteroides fragilis endophthalmitis: a case report.bacteroides fragilis is an infrequent anaerobic ocular pathogen. nevertheless, this organism is of particular significance because it may be resistant to many antibiotics that are typically effective against anaerobes. we present what is to the best of our knowledge the first reported case of b. fragilis endophthalmitis. the endophthalmitis presented in a 95-year-old man following extracapsular cataract extraction with posterior chamber intraocular lens implantation.19902191760
cardiac dysfunction in a rat model of chronic bacteremia.cardiac function was examined in vivo and in vitro in rats to determine if cardiac dysfunction could be demonstrated in a nonlethal model of infection. bacteremic rats (n = 6) had a subcutaneous polymicrobial abscess produced via repeated inoculations of an encapsulated foreign body with escherichia coli, bacteroides fragilis, and staphylococcus aureus while control rats (n = 6) had the same subcutaneous, encapsulated foreign body (an inflammatory focus) but were not inoculated with bacteria. ca ...19902192813
acetazolamide and insulin alter choroid plexus epithelial cell [na+], ph, and volume.agents that inhibit or stimulate na+ transport were tested for their effects on the ionic composition and volume of the in vivo choroid plexus (cp) epithelium. ketamine-anesthetized adult sprague-dawley rats treated 1 h with acetazolamide or insulin were analyzed for choroid cell [na+]i, [hco3-]i, and phi (dimethadione method); for transmembrane na+ and h+ gradients; and for the kinetics of penetration of 22na from plasma to plexus epithelium to csf. acetazolamide (25 mg/kg) reduced [na+]i by 5- ...19902193541
clinical manifestations of hiv infection, including persistent generalized lymphadenopathy and aids-related complex.the ability to recognize disease related to the human immunodeficiency virus (hiv) and subsequent acquired immunodeficiency syndrome (aids) has improved in recent years with refinement of laboratory techniques. because of this progress, several clinical conditions can now be identified as "pre-aids" syndromes. these include the persistent generalized lymphadenopathy syndrome, immune thrombocytopenic purpura, the wasting syndrome, and certain predominantly neurologic presentations. all are charac ...19902193949
cloning of bacteroides fragilis plasmid genes affecting metronidazole resistance and ultraviolet survival in escherichia coli.since reduced metronidazole causes dna damage, resistance to metronidazole was used as a selection method for the cloning of bacteroides fragilis genes affecting dna repair mechanisms in escherichia coli. genes from b. fragilis bf-2 were cloned on a recombinant plasmid pmt100 which made e. coli ab1157 and uvra, b, and c mutant strains more resistant to metronidazole, but more sensitive to far uv irradiation under aerobic conditions. the loci affecting metronidazole resistance and uv sensitivity ...19902194230
[in vitro antibacterial activity of lomefloxacin, a new fluoroquinolone, against hospital strains. results of a multicenter study].minimal inhibitory concentrations (mics) of lomefloxacin (lom) was determined by agar dilution for 2,819 bacterial strains isolated in 1988 in 9 university hospitals. activity of lom against nalidixic acid (nal) susceptible (s) enterobacteriaceae was close to that of pefloxacin (pef) (mode mic: 0.12-0.25 micrograms/ml); like for pef, this activity was reduced against nal resistant (r) enterobacteriaceae (mode mic: 4). mics of lom against p. aeruginosa were between 0.5 and 16 (mode: 2). lom had a ...19902195448
incidence and significance of intraperitoneal aerobic and anaerobic bacteria.intra-abdominal infections frequently occur after trauma, surgical resections, or intrinsic diseases of the gastrointestinal tract. these infections, which can be generalized or localized in intraperitoneal or extraperitoneal locations as well as in organs, are often difficult to diagnose and treat. they are usually polymicrobial, involving both aerobic and anaerobic bacteria. the average number of bacterial strains recovered is between two and five, with anaerobes dominating. the aerobic microo ...19902196123
[ways of reducing postoperative mortality and the incidence of suppurative complications in tumors of the large intestine]. 19902197491
[primary abscess of the psoas].we present in this paper a series of cases from our service of primary abscesses in psoas. we consider the appearance of immunosuppressed patients as a relevant factor of its etiopathogenicity. we believe that a c.a.t. is the best diagnostic method. all three patients that underwent open surgery have had complications. the patients treated with percutaneous puncture has shown a favourable evolution.19902198765
evaluation of the atb 32 a system for identification of anaerobic bacteria isolated from clinical specimens.a new miniaturized 4-h method for the identification of anaerobic bacteria, atb 32 a (api system sa, montalieu vercieu, france), was evaluated against conventional methods of identification. the evaluation was done by using 260 recent clinical isolates and 21 reference strains of anaerobic bacteria. all reference strains were correctly identified and did not figure in the detailed analysis. of the 140 gram-negative bacilli, 90.6% of bacteroides spp. and 95.5% of fusobacterium spp. were correctly ...19902199516
from the national institute of allergy and infectious diseases. summary of the 25th united states-japan joint conference on cholera and related diarrheal diseases. 19902201739
an update on the in vitro activity of ceftizoxime and other cephalosporin/cephamycin antimicrobial agents against clinically significant anaerobic bacteria.the in vitro activity of certain beta-lactam agents against aerobic and anaerobic bacteria is influenced by the method and medium used for susceptibility testing. the in vitro activity of ceftizoxime and cefoxitin against bacteroides fragilis group strains varies when either the broth microdilution or agar dilution method is used. in general, minimal inhibitory concentration values from broth microdilution are two- to fourfold lower than those from agar dilution. broth microdilution test results ...19902202509
gangrenous and perforated appendicitis with peritonitis: treatment and bacteriology.a comparison of single-agent antimicrobial therapy in the treatment of patients with perforated or gangrenous appendicitis and peritonitis was performed in a double-blind, randomized, prospective trial. pathologic documentation of advanced appendicitis and positive intraoperative specimen cultures were required for inclusion in the study. ceftizoxime (2 gm every 12 hours) and cefoxitin (2 gm every six hours) were compared. there were no significant differences between the treatment groups. ninet ...19902202510
in vitro activity of ljc10,627, a new carbapenem antibiotic with high stability to dehydropeptidase i.the in vitro activity of ljc10,627, a new carbapenem, was compared with those of imipenem and ceftazidime. ljc10,627 had broad-spectrum activity against gram-positive and gram-negative clinical isolates. the mics of this compound for 90% of members of the family enterobacteriaceae tested (mic90s), including strains resistant to ceftazidime, ranged from 0.1 to 25 micrograms/ml. ljc10,627 inhibited pseudomonas aeruginosa at an mic90 of 3.13 micrograms/ml; it thus was twofold more active than imipe ...19902203313
[denture stomatitis. its relation to candida albicans].one of the most usual complications of the patients that carry a prosthesis is one called stomatitis denture. its etiology is not clear enough. in this work we study ninety people, sixty of them wear prosthesis. we take samples of different places in the mouth to plate and class c. albicans. we look for the relationship of results with the clinical parameters of the patient.19902203365
[effect of the combination of estradiol-17 beta and trenbolone acetate on the plasma concentration of various metabolites and hormones in kids before a meal].a combined implant of 17 beta-estradiol trenbolone acetate in young male goats decreased blood glucose and insulin 28 d after treatment, but had no effect on growth or thyroid hormones. no relation was found between hormonal concentrations and growth performance.19902206354
a facile method for the isolation and preparation of proteins and peptides for sequence analysis in the picomolar range.we report a new and facile extraction method of proteins and polypeptides in the range of 100 to 1 kda previously separated by high-resolution sds/polyacrylamide-gel electrophoresis. proteins and polypeptides obtained by chemical or proteolytic cleavage of proteins can directly be applied to high-sensitivity n-terminal amino-acid sequence analysis by gas-phase sequencing. the coomassie blue-stained protein bands are eluted from the gel slices with 0.1 m sodium acetate buffer, ph 8.5, 0.1% sds in ...19902206456
in-vitro sensitivity tests and in-vivo efficacy of cephalosporins against bacteroides fragilis. 19902211440
alterations to the penicillin-binding proteins in the bacteroides fragilis group: a mechanism for non-beta-lactamase mediated cefoxitin resistance.the penicillin-binding proteins (pbps) of attc type strains of nine species of the bacteroides fragilis group were visualized by gel electrophoresis and subsequent fluorography. each species had a distinctive pbp pattern, although variation within species was seen. generally, five pbps could be visualized, ranging in molecular weight from approximately 40,000 to approximately 90,000. a laboratory-derived cefoxitin-resistant mutant of b. distasonis was compared with its wild type parent and cefox ...19902211448
protective effect of pgd2 against ethanol-induced gastric mucosal injury in rats.the protective effect of prostaglandin d2 (pgd2) in ethanol-, hcl-, or nacl-induced gastric mucosal injury was investigated. to clarify the mechanism, the gastric mucosal hemodynamics were also investigated using reflectance spectrophotometry and laser doppler flowmetry in anesthetized rats. pgd2 administered orally significantly reduced the ethanol-induced mucosal injury. although it did not reach any significance, pgd2 also reduced 0.6 n hcl-induced mucosal damage, and slightly reduced 25% nac ...19902212542
the bacteriology of intra-abdominal abscesses in nigerian children.the bacteriology of intra-abdominal abscess in 30 nigerian children showed anaerobic bacteria constituted 52 per cent of total microflora with bacteroides fragilis and anaerobic streptococci (19 per cent each) being predominant. e. coli and s. aureus were the predominant facultative bacteria isolated occurring in 24 and 11 per cent, respectively. the incidence of abscess was higher in males than in females and these predominant bacteria were relatively resistant to penicillins, suggesting cautio ...19902213978
septic shock complicating drainage of a bartholin gland abscess. 19902216255
beta-lactamase production and susceptibilities to amoxicillin, amoxicillin-clavulanate, ticarcillin, ticarcillin-clavulanate, cefoxitin, imipenem, and metronidazole of 320 non-bacteroides fragilis bacteroides isolates and 129 fusobacteria from 28 u.s. centers.beta-lactamase production (nitrocefin disk method) and agar dilution susceptibility of amoxicillin, amoxicillin-clavulanate, ticarcillin, ticarcillin-clavulanate, cefoxitin, imipenem, and metronidazole were determined for 320 bacteroides species (not bacteroides fragilis group) and 129 fusobacteria from 28 u.s. centers. overall, 64.7% of bacteroides species and 41.1% of fusobacteria were beta-lactamase positive. among the bacteroides species, positivity rates were highest for b. bivius (85.0%), ...19902221864
evidence that bacteroides nodosus belongs in subgroup gamma of the class proteobacteria, not in the genus bacteroides: partial sequence analysis of a b. nodosus 16s rrna gene.the taxonomic status of the anaerobe bacteroides nodosus has for some time been uncertain. to resolve this uncertainty, the distal portion of a 16s rrna gene from this important ovine pathogen was cloned, mapped, and sequenced. a comparison of the sequence with the sequences of 16s rrna molecules from other bacteria indicated that b. nodosus is more closely related to escherichia coli and other members of the class proteobacteria than to bacteroides fragilis or the bacteroides-flavobacterium-cyt ...19902223609
prevotella, a new genus to include bacteroides melaninogenicus and related species formerly classified in the genus bacteroides.it was recently proposed that the genus bacteroides should be restricted to bacteroides fragilis (the type species) and closely related organisms (viz., b. caccae, b. distasonis, b. eggerthii, b. merdae, b. ovatus, b. stercoris, b. thetaiotaomicron, b. uniformis, and b. vulgatus). by contrast, the moderately saccharolytic, predominantly oral bacteroides species, which include b. melaninogenicus, b. oralis, and related species, form a phenotypically and phylogenetically coherent group of species ...19902223612
non-immune hydrops in trisomy-18. diagnosis by vaginosonography and chorionic villus sampling in the first trimester. case report. 19902223690
non-immune hydrops in trisomy-18. diagnosis by vaginosonography and chorionic villus sampling in the first trimester. case report. 19902223690
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