| listeria infection in chinese pregnant women and neonates from shandong. | this study aims to investigate the characters of pregnant women and neonates with listeria monocytogenes infections (l. monocytogenes) in shandong of china. | 2014 | 25356132 |
| an essential role of a ferritin-like protein in acid stress tolerance of listeria monocytogenes. | the expression of ten genes of listeria monocytogenes previously identified as penicillin g-inducible was transcriptionally analyzed in the presence of 0.5 m kcl, ph 5.0 and 42 °c. this study revealed that all the genes are upregulated by osmotic stress, seven by acid stress and four by temperature stress conditions. the contribution of a gene encoding a ferritin-like protein (fri), a two-component phosphate-response regulator (phop) and an arac/xyls family transcription regulator (axyr) to temp ... | 2015 | 25352185 |
| bacterial and cellular rnas at work during listeria infection. | listeria monocytogenes is an intracellular pathogen that can enter and invade host cells. in the course of its infection, rna-mediated regulatory mechanisms provide a fast and versatile response for both the bacterium and the host. they regulate a variety of processes, such as environment sensing and virulence in pathogenic bacteria, as well as development, cellular differentiation, metabolism and immune responses in eukaryotic cells. the aim of this article is to summarize first the rna-mediate ... | 2014 | 25340833 |
| foodborne illness, australia, circa 2000 and circa 2010. | foodborne disease is a major public health problem worldwide. to examine changes in foodborne illness in australia, we estimated the incidence, hospitalizations, and deaths attributed to contaminated food circa 2010 and recalculated estimates from circa 2000. approximately 25% of gastroenteritis cases were caused by contaminated food; to account for uncertainty we used simulation techniques to estimate 90% credible intervals. we estimate that circa 2010, 4.1 million foodborne gastroenteritis cas ... | 2014 | 25340705 |
| of microspheres and microbes: a double-hit strategy for cancer immunotherapy. | efficient immunotherapy relies on the rapid generation of elevated amounts of cancer-specific t lymphocytes. we have recently demonstrated that both rapid and potent tumor-targeting immune responses can be induced with a heterologous prime-boost regimen consisting of poly-lactic co-glycolic acid (plga) microsphere-based immunization followed by listeria monocytogenes infection. | 2014 | 25340000 |
| species-specific engagement of human nucleotide oligomerization domain 2 (nod)2 and toll-like receptor (tlr) signalling upon intracellular bacterial infection: role of crohn's associated nod2 gene variants. | recognition of bacterial peptidoglycan-derived muramyl-dipeptide (mdp) by nucleotide oligomerization domain 2 (nod2) induces crucial innate immune responses. most bacteria carry the n-acetylated form of mdp (a-mdp) in their cell membranes, whereas n-glycolyl mdp (g-mdp) is typical for mycobacteria. experimental murine studies have reported g-mdp to have a greater nod2-stimulating capacity than a-mdp. as nod2 polymorphisms are associated with crohn's disease (cd), a link has been suggested betwee ... | 2015 | 25335775 |
| [immunomodulatory role of dietary lipids in an immunosuppressed mouse model and infected with listeria monocytogenes]. | dietary fatty acids immunomodulatory capacity in immunosuppression conditions may differ according to the type of fatty acid present in the diet. | 2014 | 25335671 |
| salmonella enterica serovar enteritidis and listeria monocytogenes in mango (mangifera indica l.) pulp: growth, survival and cross-contamination. | this study examined the ability of salmonella enterica serovar enteritidis and listeria monocytogenes to grow or survive in mango pulp stored at -20°c, 4°c, 10°c and 25°c, as well as to cross-contaminate mangoes by means of a knife contaminated with different levels of these pathogens. | 2014 | 25328926 |
| current status of antisense rna-mediated gene regulation in listeria monocytogenes. | listeria monocytogenes is a gram-positive human-pathogen bacterium that served as an experimental model for investigating fundamental processes of adaptive immunity and virulence. recent novel technologies allowed the identification of several hundred non-coding rnas (ncrnas) in the listeria genome and provided insight into an unexpected complex transcriptional machinery. in this review, we discuss ncrnas that are encoded on the opposite strand of the target gene and are therefore termed antisen ... | 2014 | 25325017 |
| drastic therapy for listerial brain abscess involving combined hyperbaric oxygen therapy and antimicrobial agents. | listeria monocytogenes (l. monocytogenes) is a rare causative pathogen of brain abscess that is often found in immunocompromised patients. although patients with supratentorial listerial abscesses showed a longer survival with surgical drainage, the standard therapy for patients with subtentorial lesions has not been established. | 2014 | 25324887 |
| lmo0171, a novel internalin-like protein, determines cell morphology of listeria monocytogenes and its ability to invade human cell lines. | internalins comprise a class of listeria monocytogenes proteins responsible for activation of signalling pathways leading to phagocytic uptake of the bacterium by the host cell. in this paper, a possible role of lmo0171-a new member of the internalin family was investigated. disruption of the lmo0171 gene resulted in important cell morphology alterations along with a decrease in the ability to invade three eukaryotic cell lines, that is int407, hep-2 and hela and diminished adhesion efficiency t ... | 2015 | 25323012 |
| listeria monocytogenes-associated biliary tract infections: a study of 12 consecutive cases and review. | at present, little is known regarding listeria monocytogenes-associated biliary tract infection, a rare form of listeriosis.in this article, we will study 12 culture-proven cases reported to the french national reference center for listeria from 1996 to 2013 and review the 8 previously published cases.twenty cases were studied: 17 cholecystitis, 2 cholangitis, and 1 biliary cyst infection. half were men with a median age of 69 years (32-85). comorbidities were present in 80%, including cirrhosis ... | 2014 | 25319439 |
| novel mechanism of hemin capture by hbp2, the hemoglobin-binding hemophore from listeria monocytogenes. | iron is an essential nutrient that is required for the growth of the bacterial pathogen listeria monocytogenes. in cell cultures, this microbe secretes hemin/hemoglobin-binding protein 2 (hbp2; lmo2185) protein, which has been proposed to function as a hemophore that scavenges heme from the environment. based on its primary sequence, hbp2 contains three near transporter (neat) domains of unknown function. here we show that each of these domains mediates high affinity binding to ferric heme (hemi ... | 2014 | 25315777 |
| direct incorporation of the nkt-cell activator α-galactosylceramide into a recombinant listeria monocytogenes improves breast cancer vaccine efficacy. | immune suppression in the tumour microenvironment remains a major limitation to successful immunotherapy of cancer. in the current study, we analysed whether the natural killer t cell-activating glycolipid α-galactosylceramide could overcome immune suppression and improve vaccination against metastatic breast cancer. | 2014 | 25314062 |
| the listeria monocytogenes transposon tn6188 provides increased tolerance to various quaternary ammonium compounds and ethidium bromide. | tolerance of the foodborne pathogen listeria monocytogenes to sublethal concentrations of disinfectants has been frequently reported. particularly, quaternary ammonium compounds (qacs) such as benzalkonium chloride (bc) are often used in disinfectants and also as antiseptics in food industry and hospitals. recently, we described tn6188, a novel transposon in l. monocytogenes harbouring the transporter qach, a molecular mechanism leading to increased tolerance to bc. in this study, we investigate ... | 2014 | 25312720 |
| pregnancy-related listeriosis in france, 1984 to 2011, with a focus on 606 cases from 1999 to 2011. | this study describes trends in the incidence of pregnancy-related listeriosis in france between 1984 and 2011, and presents the major characteristics of 606 cases reported between 1999 and 2011 to the french institute for public health surveillance through the mandatory notification system. the incidence of pregnancy-related listeriosis decreased by a factor of 12 from 1984 to 2011. this reduction was a result of progressive implementation of specific listeria monocytogenes control measures in f ... | 2014 | 25306879 |
| impedimetric characterization of adsorption of listeria monocytogenes on the surface of an aluminum-based immunosensor. | the impedimetric characteristics of an immunosensor depend on the electrical properties of an immunosensor substrate. the impedimetric characteristics of an immunosensor compared with adsorption of listeria monocytogenes were investigated on an aluminum surface insulated with an electrically resistive aluminum oxide layer. antibody for l. monocytogenes (anti-l. monocytogenes) was immobilized on an aluminum surface that was insulated with a native air-formed aluminum oxide layer. the resistance o ... | 2014 | 25296910 |
| detection of very long antisense transcripts by whole transcriptome rna-seq analysis of listeria monocytogenes by semiconductor sequencing technology. | the gram-positive bacterium listeria monocytogenes is the causative agent of listeriosis, a severe food-borne infection characterised by abortion, septicaemia, or meningoencephalitis. l. monocytogenes causes outbreaks of febrile gastroenteritis and accounts for community-acquired bacterial meningitis in humans. listeriosis has one of the highest mortality rates (up to 30%) of all food-borne infections. this human pathogenic bacterium is an important model organism for biomedical research to inve ... | 2014 | 25286309 |
| inhibition of listeria monocytogenes in deli-style turkey breast formulated with cultured celery powder and/or cultured sugar-vinegar blend during storage at 4°c. | fermentation-derived nitrite (no2) from vegetable sources is increasingly used as a "clean label" alternative to conventional nano2. previous results suggested that processed meats cured with no2 derived from a "natural" source had lower antimicrobial activity than did meats produced with chemical nano2; however, the differences were likely due to no2 concentration rather than source. the objective of this study was to compare the antilisterial properties of traditional and clean label alternati ... | 2014 | 25285499 |
| decontamination of hard cheeses by pulsed uv light. | cheese is a ready-to-eat food that may be contaminated on the surface by undesirable spoilage and pathogenic microorganisms during production, packaging, and postpackaging processes. penicillium roqueforti is commonly found on cheese surfaces at refrigeration temperatures and is one of the most common spoilage fungal species. consumption of cheese contaminated with listeria monocytogenes can result in foodborne listeriosis. therefore, cheese should be decontaminated at postprocessing stages. pul ... | 2014 | 25285489 |
| modeling the effects of temperature, sodium chloride, and green tea and their interactions on the thermal inactivation of listeria monocytogenes in turkey. | the interactive effects of heating temperature (55 to 65°c), sodium chloride (nacl; 0 to 2%), and green tea 60% polyphenol extract (gtpe; 0 to 3%) on the heat resistance of a five-strain mixture of listeria monocytogenes in ground turkey were determined. thermal death times were quantified in bags that were submerged in a circulating water bath set at 55, 57, 60, 63, and 65°c. the recovery medium was tryptic soy agar supplemented with 0.6% yeast extract and 1% sodium pyruvate. d-values were anal ... | 2014 | 25285486 |
| the diaphanous-related formins promote protrusion formation and cell-to-cell spread of listeria monocytogenes. | the gram-positive bacterium listeria monocytogenes is a facultative intracellular pathogen whose virulence depends on its ability to spread from cell to cell within an infected host. although the actin-related protein 2/3 (arp2/3) complex is necessary and sufficient for listeria actin tail assembly, previous studies suggest that other actin polymerization factors, such as formins, may participate in protrusion formation. here, we show that arp2/3 localized to only a minor portion of the protrusi ... | 2015 | 25281757 |
| phosphotransferase system-dependent extracellular growth of listeria monocytogenes is regulated by alternative sigma factors σl and σh. | alternative sigma (σ) factors and phosphotransferase systems (ptss) play pivotal roles in the environmental adaptation and virulence of listeria monocytogenes. the growth of the l. monocytogenes parent strain 10403s and 15 isogenic alternative σ factor mutants was assessed in defined minimal medium (dm) with pts-dependent or non-pts-dependent carbon sources at 25°c or 37°c. overall, our results suggested that the regulatory effect of alternative σ factors on the growth of l. monocytogenes is dep ... | 2014 | 25281379 |
| a novel function of interferon regulatory factor-1: inhibition of th2 cells by down-regulating the il4 gene during listeria infection. | infection with certain pathogens induces a shift of the th subset balance to a th1 dominant state. this, in turn, results in the suppression of th2 responses. we focused on the involvement of interferon regulatory factor-1 (irf-1) in the suppression of th2 cells during listeria infection. we found that the inhibition of il-4 production by th2 cells is mediated by a soluble factor (lmsn) produced by listeria-infected antigen-presenting cells. the inhibition is not observed with t cells from irf1 ... | 2015 | 25280793 |
| detection of listeria monocytogenes in food samples in avezzano, sulmona and castel di sangro (province of l'aquila, abruzzo, italy) between 2000-2009. | the retrospective study of the results of the analysed samples is a fundamental tool for the identification of major risk related to food and for planning future monitoring activities. the evaluation of the quality of data collected may also allow for estimating the effectiveness of the controls so to improve their efficacy. in this article, the authors evaluated the results of tests for the detection of listeria monocytogenes performed by the istituto zooprofilattico sperimentale dell'abruzzo e ... | 2014 | 25273959 |
| synergetic effect of combined fumaric acid and slightly acidic electrolysed water on the inactivation of food-borne pathogens and extending the shelf life of fresh beef. | to evaluate synergetic effect of slight acidic electrolysed water (saew) and fumaric acid (fa) on inactivation of total viable count (tvc) and staphylococcus aureus, listeria monocytogenes, escherichia coli o157:h7 and salmonella typhimurium in fresh beef and to study shelf life and sensory quality of beef. | 2014 | 25273314 |
| rapid and sensitive detection of listeria monocytogenes by cross-priming amplification of lmo0733 gene. | listeria monocytogenes is a food-borne pathogen that causes severe opportunistic infection in humans and animals. this study reports the development of single cross-priming amplification (s-cpa) and double cpa (d-cpa) assays targeting species-specific gene lmo0733 for identifying l. monocytogenes strains. the cpa assays were performed at a constant temperature 64 °c using seven specific primers and evaluated for specificity and sensitivity. the color change of positive amplification was directly ... | 2014 | 25273275 |
| tsc1 promotes the differentiation of memory cd8+ t cells via orchestrating the transcriptional and metabolic programs. | memory cd8(+) t cells are an essential component of protective immunity. signaling via mechanistic target of rapamycin (mtor) has been implicated in the regulation of the differentiation of effector and memory t cells. however, little is understood about the mechanisms that control mtor activity, or the effector pathways regulated by mtor. we describe here that tuberous sclerosis 1 (tsc1), a regulator of mtor signaling, plays a crucial role in promoting the differentiation and function of memory ... | 2014 | 25271321 |
| application of high resolution pyrolysis gas chromatography/mass spectrometry (hrpgc/ms) for detecting listeria monocytogenes. | the characteristic of listeria monocytogenes' pyrolysis product was found by fingerprint analysis of high resolution pyrolysis gas chromatography and mass spectrometry (hrpgc/ms), which hold a great potential to rapidly detect l. monocytogenes with the application of selected ion monitoring (sim). food products (beef and milk) contaminated by l. monocytogenes and uncontaminated were evaluated. the retention time of the characteristic peak of pyrolysis product was 19.056min, the ion of m/z were 5 ... | 2014 | 25270059 |
| σ(b) affects biofilm formation under the dual stress conditions imposed by adding salt and low temperature in listeria monocytogenes. | the food-borne pathogenic bacteria listeria monocytogenes can form biofilms on various surfaces including food-processing equipment. biofilms offer survival benefits to the organisms entrapped against environmental insults. moreover, the σ(b) transcription factor of l. monocytogenes plays an important role in its survival under various stress conditions. in this study, we evaluated whether σ(b) contributes to biofilm formation when l. monocytogenes is grown under various temperatures and media. ... | 2014 | 25269605 |
| chaperone-mediated autophagy regulates t cell responses through targeted degradation of negative regulators of t cell activation. | chaperone-mediated autophagy (cma) targets soluble proteins for lysosomal degradation. here we found that cma was activated in t cells in response to engagement of the t cell antigen receptor (tcr), which induced expression of the cma-related lysosomal receptor lamp-2a. in activated t cells, cma targeted the ubiquitin ligase itch and the calcineurin inhibitor rcan1 for degradation to maintain activation-induced responses. consequently, deletion of the gene encoding lamp-2a in t cells caused defi ... | 2014 | 25263126 |
| bacteria detection based on the evolution of enzyme-generated volatile organic compounds: determination of listeria monocytogenes in milk samples. | the rapid detection of listeria monocytogenes contamination in food is essential to prevent food-borne illness in humans. the aim of this study was to differentiate non-contaminated milk from milk contaminated with l. monocytogenes using enzyme substrates coupled with the analysis of volatile organic compounds (vocs). the method is based on the activity of β-glucosidase and hippuricase enzymes and the detection of a specific voc i.e. 2-nitrophenol and 3-fluoroaniline, respectively. vocs were ext ... | 2014 | 25263120 |
| adhesion of salmonella enteritidis and listeria monocytogenes on stainless steel welds. | pathogenic microorganisms are able to adhere on equipment surfaces, being possible to contaminate food during processing. salmonella spp. and listeria monocytogenes are important pathogens that can be transmitted by food, causing severe foodborne diseases. most surfaces of food processing industry are made of stainless steel joined by welds. however currently, there are few studies evaluating the influence of welds in the microorganism's adhesion. therefore the purpose of the present study was t ... | 2014 | 25261827 |
| whole-genome sequence of listeria monocytogenes type strain 53 xxiii. | listeria monocytogenes causes the food-borne illness listeriosis that primarily infects "vulnerable" groups (e.g., elderly adults, pregnant women, very young children, and the immunocompromised). we sequenced the genome of l. monocytogenes xxiii (atcc 15313) and assembled it into a single scaffold (three contigs) and deposited the annotated assembly into genbank as accession no. joox00000000. | 2014 | 25258271 |
| antimicrobial properties of magnesium chloride at low ph in the presence of anionic bases. | magnesium is an element essential for life and is found ubiquitously in all organisms. the different cations play important roles as enzymatic co-factors, as signaling molecules, and in stabilizing cellular components. it is not surprising that magnesium salts in microbiological experiments are typically associated with positive effects. in this study with listeria monocytogenes as a model organism, we focus however on the usefulness of magnesium (in form of mgcl2) as a stress enhancer. whereas ... | 2016 | 25252874 |
| [effect of ultra high pressure on cell membrane destruction and death of listeria monocytogenes]. | to study the effect of ultra high pressure (uhp) on the cell membrane of listeria monocytogenes. | 2014 | 25252455 |
| the adaptor asc exacerbates lethal listeria monocytogenes infection by mediating il-18 production in an inflammasome-dependent and -independent manner. | listeria monocytogenes induces the formation of inflammasomes and subsequent caspase-1 activation, and the adaptor apoptosis-associated speck-like protein containing a card (asc) is crucial for this response. however, the role of asc in l. monocytogenes infection in vivo is unclear. in this study, we demonstrate that asc has a detrimental effect on host defense against l. monocytogenes infection at a lethal dose (10(6) cfu), but not at a sublethal dose (10(3) cfu). during lethal l. monocytogenes ... | 2014 | 25251560 |
| preparation of a porcine plasma protein composite film and its application. | to use blood released from slaughtering houses, a porcine plasma protein (ppp)/nanoclay composite film was prepared. the tensile strength and elongation at break values of the ppp composite film with 5% nanoclay were 10.01 mpa and 6.55%, respectively. the ppp composite film containing 1% grapefruit seed extract (gse) was applied to pork meat, and the populations of inoculated escherichia coli o157:h7 and listeria monocytogenes in the pork meat packaged with the ppp composite film decreased by 0. ... | 2015 | 25248798 |
| retardation of listeria monocytogenes growth in mozzarella cheese using antimicrobial sachets containing rosemary oil and thyme oil. | an antimicrobial sachet containing microcellular foam starch (mfs) with embedded rosemary oil and thyme oil was developed to reduce bacterial growth in shredded mozzarella cheese. the efficacy of the volatiles of oils at various concentrations in reducing listeria monocytogenes as well as the release of the oils from the mfs have been also determined in this study. the cheese, inoculated with a cocktail of 5 strains of l. monocytogenes (approximately 3 log cfu/g), was packaged in a nylon/evoh/pe ... | 2014 | 25243791 |
| perinatal listeria monocytogenes susceptibility despite preconceptual priming and maintenance of pathogen-specific cd8(+) t cells during pregnancy. | listeria monocytogenes (lm) is an intracellular bacterium with unique predisposition for systemic maternal infection during pregnancy and morbid consequences for the developing fetus. given the high mortality associated with prenatal lm infection, strategies for augmenting protective immunity during the exceedingly vulnerable period of pregnancy are urgently needed. herein, protection conferred by attenuated lm administered before pregnancy against subsequent virulent lm prenatal infection was e ... | 2014 | 25242275 |
| the global burden of listeriosis: a systematic review and meta-analysis. | listeriosis, caused by listeria monocytogenes, is an important foodborne disease that can be difficult to control and commonly results in severe clinical outcomes. we aimed to provide the first estimates of global numbers of illnesses, deaths, and disability-adjusted life-years (dalys) due to listeriosis, by synthesising information and knowledge through a systematic review. | 2014 | 25241232 |
| listeria monocytogenes isolated from food samples from a romanian black market show distinct virulence profiles. | listeria monocytogenes is a facultative intracellular foodborne pathogen responsible for listeriosis. in a recent study, in which we investigated neglected exogenous routes of transmission of foodborne pathogens into the european union, we have isolated 15 l. monocytogenes strains in food products, which were imported from the republic of moldavia to romania and illegally sold at a local market. the aim of this study was to characterize the subtype and virulence potential of these 15 l. monocyto ... | 2015 | 25241012 |
| fisetin inhibits listeria monocytogenes virulence by interfering with the oligomerization of listeriolysin o. | listeriolysin o (llo), an essential virulence determinant of listeria monocytogenes, is a pore-forming toxin whose primary function is to facilitate cytosolic bacterial replication by breaching the phagosomal membranes, which is critical for the pathogen to evade host immune recognition. the critical role of llo in the virulence of l. monocytogenes renders it an ideal target for designing novel antivirulence therapeutics. we found that fisetin, a natural flavonoid without antimicrobial activity, ... | 2015 | 25231018 |
| old war, new battle, new fighters! | | 2015 | 25231016 |
| lmtdrm database: a comprehensive database on thiol metabolic gene/gene products in listeria monocytogenes egde. | there are a number of databases on the listeria species and about their genome. however, these databases do not specifically address a set of network that is important in defence mechanism of the bacteria. listeria monocytogenes egde is a well-established intracellular model organism to study host pathogenicity because of its versatility in the host environment. here, we have focused on thiol disulphide redox metabolic network proteins, specifically in l. monocytogenes egde. the thiol redox meta ... | 2014 | 25228549 |
| sodium chloride-induced filamentation and alternative gene expression of fts, murz, and gnd in listeria monocytogenes 08-5923 on vacuum-packaged ham. | the aim of this study was to examine the filament formation and differential gene expression of listeria monocytogenes 08-5923 grown on refrigerated vacuum-packaged ham products with various nacl concentrations. filament formation of l. monocytogenes was observed on ham products with 1.35% and 2.35% nacl, which was monitored using flow cytometry by measuring forward light scatter. quantitative real-time pcr was used to study the differential expression of genes in filamented cells of l. monocyto ... | 2014 | 25227637 |
| the p60 and nama autolysins from listeria monocytogenes contribute to host colonization and induction of protective memory. | inducing long-term protective memory cd8(+) t-cells is a desirable goal for vaccines against intracellular pathogens. however, the mechanisms of differentiation of cd8(+) t-cells into long-lived memory cells capable of mediating protection of immunized hosts remain incompletely understood. we have developed an experimental system using mice immunized with wild type (wt) or mutants of the intracellular bacterium listeria monocytogenes (lm) that either do or do not develop protective memory cd8(+) ... | 2015 | 25225110 |
| potentiometric aptasensing of listeria monocytogenes using protamine as an indicator. | exposure to pathogens in recreational or drinking water is a serious public health concern. it is important to rapidly determine and identify trace levels of pathogens in real environmental samples. we report here on a label-free potentiometric aptasensor for rapid, sensitive, and selective detection of listeria monocytogenes (lm), a pathogen widely distributed in the environment. an aptamer binds specifically to internalin a, a surface protein present in lm cells. the target-binding event preve ... | 2014 | 25220163 |
| progression to multi-scale models and the application to food system intervention strategies. | the aim of this article is to discuss how the systems science approach can be used to optimize intervention strategies in food animal systems. it advocates the idea that the challenges of maintaining a safe food supply are best addressed by integrating modeling and mathematics with biological studies critical to formulation of public policy to address these challenges. much information on the biology and epidemiology of food animal systems has been characterized through single-discipline methods ... | 2015 | 25217407 |
| phylogenetic analysis of the listeria monocytogenes based on sequencing of 16s rrna and hlya genes. | the discrimination between listeria monocytogenes and listeria species has been detected. the 16s rrna and hlya were pcr amplified with set of oligonucleotide primers with flank 1,500 and 456 bp fragments, respectively. based on the differences in 16s rrna and hlya genes, a total 80 isolates from different environmental, food and clinical samples confirmed it to be l. monocytogenes. the 16s rrna sequence similarity suggested that the isolates were similar to the previously reported ones from dif ... | 2014 | 25205124 |
| efficacy of acetic acid against listeria monocytogenes attached to poultry skin during refrigerated storage. | this work evaluates the effect of acetic acid dipping on the growth of l. monocytogenes on poultry legs stored at 4 °c for eight days. fresh inoculated chicken legs were dipped into either a 1% or 2% acetic acid solution (v/v) or distilled water (control). changes in mesophiles, psychrotrophs, enterobacteriaceae counts and sensorial characteristics (odor, color, texture and overall appearance) were also evaluated. the shelf life of the samples washed with acetic acid was extended by at least two ... | 2014 | 28234335 |
| summary of 11 years of enteric outbreak investigations and criteria to initiate an investigation, province of quebec, 2002 through 2012. | this article presents a retrospective analysis of enteric disease outbreak investigations led by or conducted in collaboration with provincial health authorities in the province of quebec from 2002 through 2012. objectives were to characterize enteric disease outbreaks, quantify and describe those for which a source was identified (including the control measures implemented), identify factors that contributed to or impeded identification of the source, and recommend areas for improvement in outb ... | 2014 | 25198848 |
| listeria monocytogenes serotype 1/2b and 4b isolates from human clinical cases and foods show differences in tolerance to refrigeration and salt stress. | control of listeria monocytogenes in food processing facilities is a difficult issue because of the ability of this microorganism to form biofilms and adapt to adverse environmental conditions. survival at high concentrations of sodium chloride and growth at refrigeration temperatures are two other important characteristics of l. monocytogenes isolates. the aim of this study was to compare the growth characteristics under stress conditions at different temperatures of l. monocytogenes serotypes ... | 2014 | 25198843 |
| thermal and nonthermal factors affecting survival of salmonella and listeria monocytogenes in animal manure-based compost mixtures. | reduction of enteric pathogens in animal manures before field application is essential for mitigating the risk of foodborne illness associated with produce. aerobic composting of manures has been advocated as an effective treatment for reducing pathogen populations, and heat is a major factor contributing to pathogen inactivation. this study was initiated to determine the potential contribution of both thermal and nonthermal (ph, volatile acids, and ammonia) factors to pathogen inactivation duri ... | 2014 | 25198842 |
| prevalence and antimicrobial susceptibility of listeria monocytogenes on chicken carcasses in bandung, indonesia. | this study was conducted to determine the prevalence and quantify the number of listeria monocytogenes in fresh chicken carcasses sold in traditional markets and supermarkets in bandung, west java, indonesia, and to determine the antimicrobial resistance patterns of the isolated l. monocytogenes strains. the overall prevalence of l. monocytogenes in chicken carcasses was 15.8% (29/184). when comparing samples from traditional markets and supermarkets, no significant difference in the l. monocyto ... | 2014 | 25198605 |
| comparison of listeria monocytogenes isolates across the island of ireland. | building a comprehensive knowledge base of the association of listeria monocytogenes isolates across national food chains, clinical cases, and environments can play a key role in helping control the incidence of listeriosis. today, many food chains cross national borders and are often shared by neighboring countries. this study characterized l. monocytogenes isolated from food samples in northern ireland and investigated whether similarities in the population and associations of l. monocytogenes ... | 2014 | 25198604 |
| low, medium, and high heat tolerant strains of listeria monocytogenes and increased heat stress resistance after exposure to sublethal heat. | a group of 37 strains representing all 13 serotypes of listeria monocytogenes with an initial cell density of 10(7) cfu/ml were analyzed for their heat tolerance at 60°c for 10 min. these l. monocytogenes strains were categorized into three heat tolerance groups: low (<2 log cfu/ml survival), medium (2 to 4 log cfu/ml survival), and high (4 to 6 log cfu/ml survival). serotype 1/2a strains had relatively low heat tolerance; seven of the eight tested strains were classified as low heat tolerant. o ... | 2014 | 25198590 |
| growth of listeria monocytogenes, salmonella spp., escherichia coli o157:h7, and staphylococcus aureus on cheese during extended storage at 25°c. | potentially hazardous foods require time/temperature control for safety. according to the u.s. food and drug administration food code, most cheeses are potentially hazardous foods based on ph and water activity, and a product assessment is required to evaluate safety of storage >6 h at 21°c. we tested the ability of 67 market cheeses to support growth of listeria monocytogenes (lm), salmonella spp. (salm), escherichia coli o157:h7 (ec), and staphylococcus aureus (sa) over 15 days at 25°c. hard ( ... | 2014 | 25198588 |
| virulence and genotypic characterization of listeria monocytogenes isolated from vegetable and soil samples. | listeria monocytogenes, a foodborne pathogen is ubiquitous to different environments including the agroecosystem. the organism poses serious public health problem. therefore, an attempt has been made to gain further insight to their antibiotic susceptibility, serotypes and the virulence genes. | 2014 | 25195727 |
| monitoring occurrence and persistence of listeria monocytogenes in foods and food processing environments in the republic of ireland. | although rates of listeriosis are low in comparison to other foodborne pathogenic illness, listeriosis poses a significant risk to human health as the invasive form can have a mortality rate as high as 30%. food processors, especially those who produce ready-to-eat (rte) products, need to be vigilant against listeria monocytogenes, the causative pathogen of listeriosis, and as such, the occurrence of l. monocytogenes in food and in the food processing environment needs to be carefully monitored. ... | 2014 | 25191314 |
| potential of cell-free supernatants from cultures of selected lactic acid bacteria and yeast obtained from local fermented foods as inhibitors of listeria monocytogenes, salmonella spp. and staphylococcus aureus. | food-borne infections cause huge economic and human life losses worldwide. the most common contaminants of foods include listeria monocytogenes salmonellae and staphylococcus aureus. l. monocytogenes is most notorious due to its tolerance to common food preservation methods and the risks it poses, including higher fatality rates. safer, more efficacious control methods are thus needed. along with food-borne pathogens, lactic acid bacteria (lab) can also be found in foods. some lab isolates inhib ... | 2014 | 25190588 |
| systemic c3 modulates cd8+ t cell contraction after listeria monocytogenes infection. | ag-specific cd8(+) t cell contraction (contraction), which occurs after the resolution of infection, is critical for homeostasis of the immune system. although complement components regulate the primary cd8(+) t cell response, there is insufficient evidence supporting their role in regulating contraction and memory. in this study, we show that c3-deficient (c3(-/-)) mice exhibited significantly less cd8(+) t cell contraction than did wild-type mice postinfection with recombinant listeria monocyt ... | 2014 | 25187659 |
| [host responses to bacterial infections]. | pathogenic bacteria and host defense system have been evolved by their offense and defense. in vivo research is crucial for elucidation of interactions between them. i have investigated their offence and defense by various standpoints using mouse models of listeria monocytogenes and staphylococcus aureus infections. herein, the results of my research including the roles of endogenous cytokines in host defense, the attenuation of host defense mechanism in obesity and diabetes, the development of ... | 2014 | 25186639 |
| an educationally inspired illustration of two-dimensional quantitative microbiological risk assessment (qmra) and sensitivity analysis. | quantitative microbiological risk assessment (qmra) is a structured methodology used to assess the risk involved by ingestion of a pathogen. it applies mathematical models combined with an accurate exploitation of data sets, represented by distributions and - in the case of two-dimensional monte carlo simulations - their hyperparameters. this research aims to highlight background information, assumptions and truncations of a two-dimensional qmra and advanced sensitivity analysis. we believe that ... | 2014 | 25173917 |
| benzalkonium chloride and heavy-metal tolerance in listeria monocytogenes from retail foods. | phenotypic and genotypic tolerance in 71 listeria monocytogenes isolates from different varieties of foods to benzalkonium chloride (bc) and cadmium were investigated by susceptibility test and molecular methods. to investigate the role of efflux pumps in bc tolerance, reserpine, an efflux pump inhibitor, was added to the bc tolerant strains. tolerance to bc and cadmium were 26.8% (19/71) and 49.3% (35/71) respectively. strains with bc tolerance were significantly more frequent among those of se ... | 2014 | 25173916 |
| listeria monocytogenes infection in inflammatory bowel disease patients: case series and review of the literature. | listeria monocytogenes (lm) is a gram-positive intracellular bacillus that in immunodeficient patients, children, geriatric patients, pregnant women, and even in healthy individuals can cause central nervous system infection, bacteremia, and other clinical manifestations, becoming a relevant pathogen. | 2014 | 25171025 |
| agonistic anti-cd40 enhances the cd8+ t cell response during vesicular stomatitis virus infection. | intracellular pathogens are capable of inducing vigorous cd8+ t cell responses. however, we do not entirely understand the factors driving the generation of large pools of highly protective memory cd8+ t cells. here, we studied the generation of endogenous ovalbumin-specific memory cd8+ t cells following infection with recombinant vesicular stomatitis virus (vsv) and listeria monocytogenes (lm). vsv infection resulted in the generation of a large ovalbumin-specific memory cd8+ t cell population, ... | 2014 | 25166494 |
| listeria rhomboencephalitis in a patient on a tumor necrosis factor α inhibitor (etanercept). | we describe a case of an 87-year-old woman with rheumatoid arthritis on etanercept who developed subacute encephalopathy. magnetic resonance imaging and blood cultures led to the diagnosis of listeria monocytogenes rhomboencephalitis, which proved to be fatal. postmortem examination of the brain revealed abscesses with extensive necrosis. gram stain showed small gram-positive rods in the necrotic tissue and the wall lining the abscesses. while rhomboencephalitis is a rare entity, and clinical re ... | 2014 | 25160017 |
| listeria monocytogenes is resistant to lysozyme through the regulation, not the acquisition, of cell wall-modifying enzymes. | listeria monocytogenes is a gram-positive facultative intracellular pathogen that is highly resistant to lysozyme, a ubiquitous enzyme of the innate immune system that degrades cell wall peptidoglycan. two peptidoglycan-modifying enzymes, pgda and oata, confer lysozyme resistance on l. monocytogenes; however, these enzymes are also conserved among lysozyme-sensitive nonpathogens. we sought to identify additional factors responsible for lysozyme resistance in l. monocytogenes. a forward genetic s ... | 2014 | 25157076 |
| dose response of listeria monocytogenes invasion, fetal morbidity, and fetal mortality after oral challenge in pregnant and nonpregnant mongolian gerbils. | listeria monocytogenes is a food-borne pathogen that can result in adverse pregnancy outcomes, such as stillbirth or premature delivery. the mongolian gerbil was recently proposed as the most appropriate small-animal model of listeriosis due to its susceptibility to the same invasion pathways as humans. the objectives of this study were to investigate invasion and adverse pregnancy outcomes in gerbils orally exposed to l. monocytogenes, to compare the dose-response data to those of other animal ... | 2014 | 25156729 |
| potential uptake of escherichia coli o157:h7 and listeria monocytogenes from growth substrate into leaves of salad plants and basil grown in soil irrigated with contaminated water. | outbreaks of foodborne illness, resulting from the consumption of fresh produce contaminated with human pathogens, are increasing. potential uptake and persistence of human pathogens within edible parts of consumed fresh vegetables become an important issue in food safety. this study was conducted to assess the potential uptake and internalization of escherichia coli o157:h7 and listeria monocytogenes from an autoclaved substrate into edible parts of basil and baby salad plants (lettuce, cultiva ... | 2014 | 25150671 |
| listeria monocytogenes meningoencephalitis during the treatment with rituximab and mycophenolate mofetil in a patient with pediatric-onset systemic lupus erythematosus. | | 2016 | 25150501 |
| anti-listerial synergism of leaf essential oil of metasequoia glyptostroboides with nisin in whole, low and skim milks. | to examine the individual and synergistic anti-listerial effect of nisin and leaf essential oil of metasequoia glyptostroboides (m. glyptostroboides) against one of the leading foodborne pathogens listeria monocytogenes (l. monocytogenes) atcc 19116 in milk samples. | 2014 | 25149373 |
| antibacterial effect of silver nanoparticles against four foodborne pathogens. | there is increased demand for improved disinfection methods due to microorganisms resistant to multiple antimicrobial agents. numerous types of disinfectants are available with different properties; but the proper disinfectant must be carefully selected for any specific application to obtain the desired antimicrobial effect. | 2014 | 25147658 |
| impact of sod on the expression of stress-related genes in listeria monocytogenes 4b g with/without paraquat treatment. | listeria monocytogenes is a foodborne pathogen that causes listeriosis. paraquat can generate reactive oxygen species (ros) in cells, which results in oxidative stress. it was first shown that 1 mm of paraquat inhibited the growth rate of a superoxide dismutase (sod)-deletion mutant (∆sod) generated from l. monocytogenes 4b g but not in the wild-type, and induced the expression of other resistance genes (kat, fri, perr, sigb, and reca) as well as sod in the wild type. interestingly, without para ... | 2014 | 25146690 |
| combination of pulsed electric fields, mild heat and essential oils as an alternative to the ultrapasteurization of liquid whole egg. | the production of microbiologically safe liquid whole egg (lwe) by industrial ultrapasteurization is restricted by the high thermal sensitivity of lwe components. this research proposes an alternative treatment based on the application of pulsed electric fields (pef) and mild heat, in the presence of natural essential oils (eos) or their individual components (ics). the obtained results indicate that the successive application of pef (25kv/ and 100kj/kg) followed by heat (60°c during 3.5') to lw ... | 2014 | 25146463 |
| riboswitches. sequestration of a two-component response regulator by a riboswitch-regulated noncoding rna. | riboswitches are ligand-binding elements contained within the 5' untranslated regions of bacterial transcripts, which generally regulate expression of downstream open reading frames. here, we show that in listeria monocytogenes, a riboswitch that binds vitamin b12 controls expression of a noncoding regulatory rna, rli55. rli55, in turn, controls expression of the eut genes, whose products enable ethanolamine utilization and require b12 as a cofactor. defects in ethanolamine utilization, or in it ... | 2014 | 25146292 |
| choice of reference sequence and assembler for alignment of listeria monocytogenes short-read sequence data greatly influences rates of error in snp analyses. | the wide availability of whole-genome sequencing (wgs) and an abundance of open-source software have made detection of single-nucleotide polymorphisms (snps) in bacterial genomes an increasingly accessible and effective tool for comparative analyses. thus, ensuring that real nucleotide differences between genomes (i.e., true snps) are detected at high rates and that the influences of errors (such as false positive snps, ambiguously called sites, and gaps) are mitigated is of utmost importance. t ... | 2014 | 25144537 |
| genipin cross-linked nanocomposite films for the immobilization of antimicrobial agent. | cellulose nanocrystal (cnc) reinforced chitosan based antimicrobial films were prepared by immobilizing nisin on the surface of the films. nanocomposite films containing 18.65 μg/cm(2) of nisin reduced the count of l. monocytogenes by 6.73 log cfu/g, compared to the control meat samples (8.54 log cfu/g) during storage at 4 °c in a ready-to-eat (rte) meat system. film formulations containing 9.33 μg/cm(2) of nisin increased the lag phase of l. monocytogenes on meat by more than 21 days, whereas f ... | 2014 | 25140839 |
| [listeria monocytogenes and its relationship with non-biological therapy in inflammatory bowel disease]. | | 2017 | 25138387 |
| the influence of subminimal inhibitory concentrations of benzalkonium chloride on biofilm formation by listeria monocytogenes. | disinfectants, such as benzalkonium chloride (bac), are commonly used to control listeria monocytogenes and other pathogens in food processing plants. prior studies have demonstrated that the resistance to bac of l. monocytogenes was associated with the prolonged survival of three strains of molecular serotype 1/2a in an iberian pork processing plant. because survival in such environments is related to biofilm formation, we hypothesised that the influence of bac on the biofilm formation potentia ... | 2014 | 25136789 |
| l. monocytogenes in a cheese processing facility: learning from contamination scenarios over three years of sampling. | the aim of this study was to analyze the changing patterns of listeria monocytogenes contamination in a cheese processing facility manufacturing a wide range of ready-to-eat products. characterization of l. monocytogenes isolates included genotyping by pulsed-field gel electrophoresis (pfge) and multi-locus sequence typing (mlst). disinfectant-susceptibility tests and the assessment of l. monocytogenes survival in fresh cheese were also conducted. during the sampling period between 2010 and 2013 ... | 2014 | 25136788 |
| cytotoxicity of purified listeriolysin o on mouse and human leukocytes and leukaemia cells. | listeriolysin o (llo) is the main virulence factor of listeria monocytogenes and facilitates the intracellular survival of the pathogen. some of its characteristics endorse the growing popularity of llo for use in biotechnology, particularly in the development of novel vaccines. here, we evaluate the use of llo to eradicate leukaemia cells. | 2014 | 25134983 |
| structural and mechanistic insight into the listeria monocytogenes two-enzyme lipoteichoic acid synthesis system. | lipoteichoic acid (lta) is an important cell wall component required for proper cell growth in many gram-positive bacteria. in listeria monocytogenes, two enzymes are required for the synthesis of this polyglycerolphosphate polymer. the lta primase ltap(lm) initiates lta synthesis by transferring the first glycerolphosphate (grop) subunit onto the glycolipid anchor and the lta synthase ltas(lm) extends the polymer by the repeated addition of grop subunits to the tip of the growing chain. here, w ... | 2014 | 25128528 |
| identification, characterization, immobilization of a novel type hydrolase (lmh) from listeria monocytogenes. | a novel type of hydrolase (lmh) from listeria monocytogenes was identified, characterized, and immobilized for biotechnological applications. primary sequence analysis indicated that lmh had a catalytic triad (ser(91)-asp(192)-his(222)) with a molecular weight of 27.8 kda. homologs of this enzyme are produced by many gram-positive bacteria including bacillus, staphylococcus, and enterococcus. biochemical properties of lmh were investigated by performing mass spectrometry, dynamic light scatterin ... | 2015 | 25128099 |
| is the exoproteome important for bacterial pathogenesis? lessons learned from interstrain exoprotein diversity in listeria monocytogenes grown at different temperatures. | bacterial exoproteomes vary in composition and quantity among species and within each species, depending on the environmental conditions to which the cells are exposed. this article critically reviews the literature available on exoproteins synthesized by the foodborne pathogenic bacterium listeria monocytogenes grown at different temperatures. the main challenges posed for exoproteome analyses and the strategies that are being used to overcome these constraints are discussed. over thirty exopro ... | 2014 | 25127015 |
| listerial rhombencephalitis in an immunocompetent woman. | listeriosis usually affects immunocompromised patients including elderly people and pregnant women, but it may also affect otherwise healthy individuals. in our report, we present a case of a rare and very severe form of listeriosis-rhombencephalitis in a 61-year-old female with no history of immunosuppression, who, because of history, clinical picture, and laboratory results as well as negative cultures, was at first diagnosed with viral encephalitis. this paper underlines that listeria monocyt ... | 2014 | 25126431 |
| 3,4-methylenedioxymethamphetamine (mdma--ecstasy) decreases neutrophil activity through the glucocorticoid pathway and impairs host resistance to listeria monocytogenes infection in mice. | ecstasy is the popular name of the abuse drug 3,4-methylenedioxymethamphetamine (mdma) that decreases immunity in animals. the mechanisms that generate such alterations are still controversial. seven independent pharmacological approaches were performed in mice to identify the possible mechanisms underlying the decrease of neutrophil activity induced by mdma and the possible effects of mdma on host resistance to listeria monocytogenes. our data showed that mdma (10 mg kg(-1)) administration decr ... | 2014 | 25113903 |
| enhanced inactivation of food-borne pathogens in ready-to-eat sliced ham by near-infrared heating combined with uv-c irradiation and mechanism of the synergistic bactericidal action. | the objective of the study described in this article was, first, to investigate the effect of the simultaneous application of near-infrared (nir) heating and uv irradiation on inactivation of escherichia coli o157:h7, salmonella enterica serovar typhimurium, and listeria monocytogenes in ready-to-eat (rte) sliced ham and as well as its effect on product quality and, second, to elucidate the underlying mechanisms of the synergistic bactericidal action of nir heating and uv irradiation. with the i ... | 2015 | 25107964 |
| the first invertebrate rig-i-like receptor (rlr) homolog gene in the pacific oyster crassostrea gigas. | retinoic acid-inducible gene i (rig-i)-like receptor (rlr) is a pivotal receptor that detects numerous rna and dna viruses and mediates the innate induction of interferons and pro-inflammatory cytokines upon viral infection. in the present study, we cloned and characterized the first rig-i gene in a marine mollusk, crassostrea gigas, and designated it as cgrig-i. the full-length cgrig-i cdna is 3436 bp, including 5'- and 3'-untranslated regions (utrs) of 93 bp and 286 bp, respectively, and an op ... | 2014 | 25107697 |
| effect of lauric arginate, nisin z, and a combination against several food-related bacteria. | the effects of lauric arginate (lae) and nisin z, alone or in combination, on cell damage were investigated against escherichia coli o157:h7, listeria monocytogenes and brochothrix thermosphacta, by scanning electron microscopy (sem) and transmission electron microscopy (tem) observations, efflux of potassium and phosphate ions, and growth inhibition. a combination of lae with nisin z caused severe and dramatic changes in the cytoplasmic membrane and cell lysis of both gram-positive and gram-neg ... | 2014 | 25106040 |
| cyclic di-gmp-dependent signaling pathways in the pathogenic firmicute listeria monocytogenes. | we characterized key components and major targets of the c-di-gmp signaling pathways in the foodborne pathogen listeria monocytogenes, identified a new c-di-gmp-inducible exopolysaccharide responsible for motility inhibition, cell aggregation, and enhanced tolerance to disinfectants and desiccation, and provided first insights into the role of c-di-gmp signaling in listerial virulence. genome-wide genetic and biochemical analyses of c-di-gmp signaling pathways revealed that l. monocytogenes has ... | 2014 | 25101646 |
| management of pregnant women with presumptive exposure to listeria monocytogenes. | : listeriosis is predominantly a foodborne illness, with sporadic and outbreak-related cases tied to consumption of food contaminated with listeria (listeria monocytogenes). the incidence of listeriosis associated with pregnancy is approximately 13 times higher than in the general population. maternal infection may present as a nonspecific, flu-like illness with fever, myalgia, backache, and headache, often preceded by diarrhea or other gastrointestinal symptoms. however, fetal and neonatal infe ... | 2014 | 25098360 |
| pediococcus pentosaceus sb83 as a potential probiotic incorporated in a liquid system for vaginal delivery. | pediococcus pentosaceus sb83 is a bacteriocinogenic culture having potential use as a vaginal probiotic. the objective of this study was to evaluate the behaviour of p. pentosaceus sb83 incorporated in a liquid system for eventually formulating a gel for vaginal delivery. the vaginal probiotic incorporated into glycerol was able to survive in simulated vaginal fluid at normal vaginal ph (4.2) and at ph of vaginal infections (5.5 and 6.5). the probiotic can be stored at 4 °c for a longer period o ... | 2014 | 25097107 |
| small molecule deubiquitinase inhibitors promote macrophage anti-infective capacity. | the global spread of anti-microbial resistance requires urgent attention, and diverse alternative strategies have been suggested to address this public health concern. host-directed immunomodulatory therapies represent one approach that could reduce selection for resistant bacterial strains. recently, the small molecule deubiquitinase inhibitor wp1130 was reported as a potential anti-infective drug against important human food-borne pathogens, notably listeria monocytogenes and noroviruses. util ... | 2014 | 25093325 |
| integrated air stream micromixer for performing bioanalytical assays on a plastic chip. | this paper describes the design, functioning and use of an integrated mixer that relies on air flux to agitate microliter entities of fluid in an embedded microfluidic cavity. the system was fabricated from multiple layers of a thermoplastic elastomer and features circuits for both liquid and air supply along with pneumatic valves for process control. internally-dyed polymer particles have been used to visualize flow within the fluid phase during agitation. numerical modelling of the micromixer ... | 2014 | 25091476 |
| impact of rli87 gene deletion on response of listeria monocytogenes to environmental stress. | listeria monocytogenes (lm) is a zoonotic pathogen that widely adapts to various environments. recent studies have found that noncoding rnas (ncrnas) play regulatory roles in lm responses to environmental stress. to understand the role of ncrna rli87 in the response regulation, a rli87 deletion strain lm-δrli87 was constructed by homologous recombination and tested for stress responses to high temperature, low temperature, high osmotic pressure, alcohol, acidity, alkaline and oxidative environme ... | 2014 | 25091276 |
| genomic characterization and expression analysis of five novel il-17 genes in the pacific oyster, crassostrea gigas. | interleukin-17 (il-17) is a proinflammatory cytokine that plays an important role in clearing extracellular bacteria and contributes to the pathology of many autoimmune and allergic conditions. in the present study, five novel il-17 homologs were identified by searching and analyzing the pacific oyster genome. all six cgil-17 members (including a previously reported homolog) contained four conserved cysteines that were used in the formation of disulfide bonds. phylogenetic analysis showed that a ... | 2014 | 25090939 |
| use of antimicrobial films and edible coatings incorporating chemical and biological preservatives to control growth of listeria monocytogenes on cold smoked salmon. | the relatively high incidence of listeria monocytogenes in cold smoked salmon (css) is of concern as it is a refrigerated processed food of extended durability (repfed). the objectives of this study were to compare and optimize the antimicrobial effectiveness of films and coatings incorporating nisin (nis) and sodium lactate (sl), sodium diacetate (sd), potassium sorbate (ps), and/or sodium benzoate (sb) in binary or ternary combinations on css. surface treatments incorporating nis (25000 iu/ml) ... | 2014 | 25089272 |
| development of double loop-mediated isothermal amplification to detect listeria monocytogenes in food. | in this study, a double loop-mediated isothermal amplification (dlamp) based on two target genes hlya and iap was developed for the rapid detection of listeria monocytogenes in food. the results revealed that the detection time and temperature of our dlamp assay for l. monocytogenes were 15 min and 63 °c respectively, with a sensitivity of 10 fg dna of l. monocytogenes per tube. while normal lamp (nlamp) of hlya or iap was 100 fg dna of l. monocytogenes per tube for 45 min and 63 °c. furthermore ... | 2014 | 25086581 |