| analysis of older adults' domestic kitchen storage practices in the united kingdom: identification of risk factors associated with listeriosis. | increased listeriosis incidence among older adults (≥ 60 years) has been reported internationally, with many cases reported to be sporadic and associated with ready-to-eat (rte) food products with extended refrigerated shelf life. given that the home kitchen is recognized as a significant location where foodborne illnesses are acquired, it is important that consumers implement safe food practices to minimize risks. this is crucial for vulnerable consumers, such as older adults. consumer food saf ... | 2015 | 25836399 |
| evaluation of novel micronized encapsulated essential oil-containing phosphate and lactate blends for growth inhibition of listeria monocytogenes and salmonella on poultry bologna, pork ham, and roast beef ready-to-eat deli loaves. | essential oils and their constituents are reported to possess potent antimicrobial activity, but their use in food processing is limited because of low solubility in aqueous systems and volatilization during processing. two proprietary noncommercial essential oil-containing phosphate blends were evaluated for antimicrobial activity against salmonella enterica cocktail (sc)-and listeria monocytogenes (lm)-inoculated deli meat products made from pork, poultry, or beef. four treatments were tested ... | 2015 | 25836394 |
| serotypes and pulsotypes diversity of listeria monocytogenes in a beef-processing environment. | utensils and equipment from meat-processing facilities are considered relevant cross-contamination points of listeria monocytogenes to foods, demanding tracking studies to identify their specific origins, and predict proper control. the present study aimed to detect l. monocytogenes in a beef-processing facility, investigating the diversity of serotypes and pulsotypes in order to identify the possible contamination routes. surface samples from knives (n=26), tables (n=78), and employees hands (n ... | 2015 | 25835809 |
| efficacy of cell wall-deficient spheroplasts against experimental murine listeriosis. | various strategies adapted to develop an efficient vaccine against foodborne pathogen, listeria monocytogenes, have met with little success. spheroplasts (bacterial cell devoid of cell wall) are likely to undergo membrane-membrane fusion, leading to the delivery of their content to the cytosol of antigen-presenting cells, thus facilitating mhc class i antigen processing and presentation. in this study, we evaluated the prophylactic potential of listeria spheroplast-based vaccine against experime ... | 2015 | 25833403 |
| colovesical fistula presenting as listeria monocytogenes bacteraemia. | we present a case of colovesical fistula presenting with a clinical syndrome of urosepsis subsequently demonstrated to be due to listeria monocytogenes bacteraemia. the patient had a history of previous rectal cancer with a low anterior resection and a covering ileostomy that had been reversed 6 months prior to this presentation. l. monocytogenes was also isolated among mixed enteric organisms on urine culture. there were no symptoms or signs of acute gastrointestinal listeriosis or meningoencep ... | 2015 | 25827919 |
| genetic characteristics of japanese clinical listeria monocytogenes isolates. | listeria monocytogenes causes foodborne illnesses through consumption of ready-to-eat foods. although 135-201annual listeriosis cases have been estimated in japan, the details regarding the clinical isolates such as infection source, virulence level, and other genetic characteristics, are not known. in order to uncover the trends of listeriosis in japan and use the knowledge for prevention measures to be taken, the genetic characteristics of the past human clinical isolates needs to be elucidate ... | 2015 | 25826318 |
| bacteriophage predation promotes serovar diversification in listeria monocytogenes. | listeria monocytogenes is a bacterial pathogen classified into distinct serovars (svs) based on somatic and flagellar antigens. to correlate phenotype with genetic variation, we analyzed the wall teichoic acid (wta) glycosylation genes of sv 1/2, 3 and 7 strains, which differ in decoration of the ribitol-phosphate backbone with n-acetylglucosamine (glcnac) and/or rhamnose. inactivation of lmo1080 or the dtdp-l-rhamnose biosynthesis genes rmlacbd (lmo1081-1084) resulted in loss of rhamnose, where ... | 2015 | 25825127 |
| the human p-glycoprotein transporter enhances the type i interferon response to listeria monocytogenes infection. | human multidrug efflux transporters are known for their ability to extrude antibiotics and toxic compounds out of cells, yet accumulating data indicate they have additional functions in diverse physiological processes not related to drug efflux. here, we show that the human multidrug transporter p-glycoprotein (p-gp) (also named mdr1 and abcb1) is transcriptionally induced in the monocytic cell line thp-1 upon infection with the human intracellular bacterial pathogen listeria monocytogenes. nota ... | 2015 | 25824830 |
| identification of a peptide-pheromone that enhances listeria monocytogenes escape from host cell vacuoles. | listeria monocytogenes is a gram-positive facultative intracellular bacterial pathogen that invades mammalian cells and escapes from membrane-bound vacuoles to replicate within the host cell cytosol. gene products required for intracellular bacterial growth and bacterial spread to adjacent cells are regulated by a transcriptional activator known as prfa. prfa becomes activated following l. monocytogenes entry into host cells, however the signal that stimulates prfa activation has not yet been de ... | 2015 | 25822753 |
| the listeria monocytogenes strain 10403s biocyc database. | listeria monocytogenes is a food-borne pathogen of humans and other animals. the striking ability to survive several stresses usually used for food preservation makes l. monocytogenes one of the biggest concerns to the food industry, while the high mortality of listeriosis in specific groups of humans makes it a great concern for public health. previous studies have shown that a regulatory network involving alternative sigma (σ) factors and transcription factors is pivotal to stress survival. ho ... | 2015 | 25819074 |
| tumor necrosis factor-α, interleukins-12(p40), 6, and 10 levels in cerebrospinal fluid and outcome prediction in ossimi sheep with encephalitic listeriosis. | encephalitic listeriosis in sheep is a life-threatening disease. however, little is known about the cytokine response and their predictive value in this disease. the aim of present study was to assess the prognostic significance of tumor necrosis factor-α (tnf-α), interleukin-12(p40) (il-12 p40), interleukin-6 (il-6), and interleukin 10 (il-10) levels in cerebrospinal fluid (csf) in sheep with encephalitic listeriosis. fifty-nine ewes in 14 flocks were diagnosed clinically as having listeriosis. ... | 2015 | 25817420 |
| [morphologic changes in listeria cells under the effect of metabolites of human intestinal microflora enterococci]. | characterization of morphologic changes in listeria cells under the effect of metabolites of human intestine microflora enterococci. | 2015 | 25816518 |
| ascorbic acid, ultraviolet c rays, and glucose but not hyperthermia are elicitors of human β-defensin 1 mrna in normal keratinocytes. | hosts' innate defense systems are upregulated by antimicrobial peptide elicitors (apes). our aim was to investigate the effects of hyperthermia, ultraviolet a rays (uva), and ultraviolet c rays (uvc) as well as glucose and ascorbic acid (aa) on the regulation of human β-defensin 1 (defb1), cathelicidin (camp), and interferon-γ (ifng) genes in normal human keratinocytes (nhk). the indirect in vitro antimicrobial activity against staphylococcus aureus and listeria monocytogenes of these potential ... | 2015 | 25815330 |
| a naturally occurring prfa truncation in a listeria monocytogenes field strain contributes to reduced replication and cell-to-cell spread. | listeria (l.) monocytogenes is an environmental bacterium that may become an intracellular pathogen upon ingestion to cause gastroenteritis, septicaemia, abortions, and/or fatal infections of the central nervous system. we here describe a l. monocytogenes field strain (jf5171) isolated from a bovine placenta in the context of abortion, which exhibited attenuation in bovine brain-slice cultures. the whole genome of strain jf5171 was sequenced, and the invasion, replication, and intercellular spre ... | 2015 | 25813546 |
| characterization of listeria monocytogenes from three countries and antibiotic resistance differences among countries and listeria monocytogenes serogroups. | a total of 104 listeria monocytogenes isolates from 330 fish samples from three countries were characterized by multiplex pcr for serogrouping and virulence markers determination and tested for antibiotics resistance. a 53·8% of the isolates belonged to serogroup 1/2a, 3a; 32% belonged to 1/2b, 3b, 7; 14·4% belonged to 4b, 4d, 4e and 1% belonged to 1/2c, 3c. all isolates exhibited resistance to at least one antibiotic but the resistance rates varied among countries. the isolates exhibited high r ... | 2015 | 25808878 |
| antimicrobial effects of essential oils, nisin, and irradiation treatments against listeria monocytogenes on ready-to-eat carrots. | the study aimed at using essential oil (eo) alone or combined eo with nisin and low dose γ-irradiation to evaluate their antibacterial effect against listeria monocytogenes during storage of carrots at 4 °c. minicarrots were inoculated with l. monocytogenes at a final concentration of approximately 7 log cfu/g. inoculated samples were coated by nisin at final concentration of 10(3) international unit (iu)/ml or individual mountain savory eo or carvacrol at final concentration of 0.35%, w/w) or n ... | 2015 | 25807882 |
| division of human listeria monocytogenes pulsed-field gel electrophoresis (pfge) types belonging to lineage i (serovar 4b, 1/2b, and 3b) into pfge groups. | the 63 pulsed-field gel electrophoresis (pfge) types identified among 427 clinical isolates of listeria monocytogenes that were characterized in a previous study by serotyping and pfge (asci) could be further divided into 17 pfge groups. while the 63 pfge types, all part of lineage i, were established based on the number and distribution of all bands in each dna profile, the 17 pfge groups were based on the configuration of small bands with sizes <145.5 kb. the 30 pfge types of l. monocytogenes ... | 2015 | 25803595 |
| pneumonia by listeria monocytogenes: a common infection by an uncommon pathogen. | infections by listeria monocytogenes typically occur in infants, the elderly, pregnant women, and immunosuppressed subjects. pulmonary infections in adults are extremely uncommon and only few reports can be found in the literature. we here report a case of listeria pneumonia in an 85-year-old female patient and we discuss our diagnostic and therapeutic approach. despite being rare and in most cases difficult to be identified, listeria pneumonia should always be considered in immunosuppressed pat ... | 2015 | 25802774 |
| fournier's gangrene caused by listeria monocytogenes as the primary organism. | a 70-year-old man with a history of tongue cancer presented with fournier's gangrene caused by listeria monocytogenes serotype 4b. surgical debridement revealed undiagnosed rectal adenocarcinoma. the patient did not have an apparent dietary or travel history but reported daily consumption of sashimi (raw fish). old age and immunodeficiency due to rectal adenocarcinoma may have supported the direct invasion of l monocytogenes from the tumour. the present article describes the first reported case ... | 2017 | 25798155 |
| molecular serotyping and pathogenic potential of listeria monocytogenes isolated from milk and milk products in tamil nadu, india. | listeria monocytogenes, an important bacterial pathogen, is responsible for foodborne illnesses worldwide. examination of food samples for the presence of l. monocytogenes and assessment of their pathogenicity is usually an effective strategy in the prevention of listeriosis. in the present study, we have tested 307 samples of milk and milk products from various places in tamil nadu, india for the presence of l. monocytogenes using iso 11290 and u.s. food and drug administration bacteriological ... | 2015 | 25793931 |
| complete genome sequence of the persistent listeria monocytogenes strain r479a. | the complete genome sequence of the persistent listeria monocytogenes strain r479a isolated from smoked salmon in denmark and belonging to lineage ii, serovar 1/2a, and multilocus sequence type 8 (st8) is presented here. | 2015 | 25792065 |
| sensitive enumeration of listeria monocytogenes and other listeria species in various naturally contaminated matrices using a membrane filtration method. | for the enumeration of listeria monocytogenes (l. monocytogenes) in food, a sensitive enumeration method has been recently developed. this method is based on a membrane filtration of the food suspension followed by transfer of the filter on a selective medium to enumerate l. monocytogenes. an evaluation of this method was performed with several categories of foods naturally contaminated with l. monocytogenes. the results obtained with this technique were compared with those obtained from the mod ... | 2015 | 25791005 |
| a novel gene, lstc, of listeria monocytogenes is implicated in high salt tolerance. | listeria monocytogenes, causative agent of human listeriosis, has been isolated from a wide variety of foods including deli meats, soft cheeses, cantaloupes, sprouts and canned mushrooms. standard control measures for restricting microbial growth such as refrigeration and high salt are often inadequate as l. monocytogenes grows quite well in these environments. in an effort to better understand the genetic and physiological basis by which l. monocytogenes circumvents these controls, a transposon ... | 2015 | 25790994 |
| enhancing the antibacterial effect of 461 and 521 nm light emitting diodes on selected foodborne pathogens in trypticase soy broth by acidic and alkaline ph conditions. | light emitting diodes (leds) with their antibacterial effect present a novel method for food preservation. this effect may be influenced by environmental conditions such as the ph of the food contaminated by the pathogen. thus, it is necessary to investigate the influence of ph on the antibacterial effect of leds before their application to real food matrices. escherichia coli o157:h7, salmonella typhimurium and listeria monocytogenes in trypticase soy broth were illuminated using 10-w 461 (22.1 ... | 2015 | 25790991 |
| effects of oxygen-depleted atmospheres on survival and growth of listeria monocytogenes on fresh-cut iceberg lettuce stored at mild abuse commercial temperatures. | the effects of oxygen-depleted atmospheres, 0.25% o2+12% co2 (balance n2) and 2% o2 + 6% co2 (balance n2), on growth of listeria monocytogenes on fresh-cut iceberg lettuce were determined. the study was carried out at mild abuse temperatures using controlled atmosphere chambers. during storage at a constant temperature of 7 °c, growth was enhanced at the lower oxygen level of 0.25% o2 by day 10. over 17 days of storage at temperatures designed to mimic mild abuse commercial conditions, there wer ... | 2015 | 25790986 |
| notes from the field: listeriosis associated with stone fruit--united states, 2014. | on july 19, 2014, a packing company in california (company a) voluntarily recalled certain lots of stone fruits, including whole peaches, nectarines, plums, and pluots, because of concern about contamination with listeria monocytogenes based on internal company testing. on july 31, the recall was expanded to cover all fruit packed at their facility during june 1-july 17. after the initial recall, clinicians, state and local health departments, cdc, and the food and drug administration (fda) rece ... | 2015 | 25789745 |
| combined effect of a nanoemulsion of d-limonene and nisin on listeria monocytogenes growth and viability in culture media and foods. | the present work evaluated the antibacterial effect of nanoemulsions from natural compounds d-limonene and nisin against listeria monocytogenes in tryptic soy broth growth medium, chicken broth, and vegetable cream. experiments were performed by means of optical density growth curves and studies of viability in culture media and foods. stability of nanoemulsions was evaluated by a mastersizer 2000 equipment. results showed greater effectiveness when applying d-limonene in form of nanoemulsion th ... | 2016 | 25788168 |
| estimating the attack rate of pregnancy-associated listeriosis during a large outbreak. | in 2011, a multistate outbreak of listeriosis linked to contaminated cantaloupes raised concerns that many pregnant women might have been exposed to listeria monocytogenes. listeriosis during pregnancy can cause fetal death, premature delivery, and neonatal sepsis and meningitis. little information is available to guide healthcare providers who care for asymptomatic pregnant women with suspected l. monocytogenes exposure. | 2015 | 25784782 |
| a new bovine conjunctiva model shows that listeria monocytogenes invasion is associated with lysozyme resistance. | listerial keratoconjunctivitis ('silage eye') is a wide spread problem in ruminants causing economic losses to farmers and impacts negatively on animal welfare. it results from direct entry of listeria monocytogenes into the eye, often following consumption of contaminated silage. an isolation protocol for bovine conjunctival swabbing was developed and used to sample both infected and healthy eyes bovine eyes (n=46). l. monocytogenes was only isolated from one healthy eye sample, and suggests th ... | 2015 | 25778543 |
| stability of sublethal acid stress adaptation and induced cross protection against lauric arginate in listeria monocytogenes. | the stability of acid stress adaptation in listeria monocytogenes and its induced cross protection effect against gras (generally recognized as safe) antimicrobial compounds has never been investigated before. in the present study, the acid stress adaptation in l. monocytogenes was initially induced in ph 5.0 tryptic soy broth supplemented with 0.6% yeast extract (tsb-ye) at 37 °c. subsequently, the stability of acid stress adaptation, which was defined as the capacity to maintain its acquired a ... | 2015 | 25777680 |
| irf8 directs stress-induced autophagy in macrophages and promotes clearance of listeria monocytogenes. | autophagy, activated by many stresses, plays a critical role in innate immune responses. here we show that interferon regulatory factor 8 (irf8) is required for the expression of autophagy-related genes in dendritic cells. furthermore in macrophages, irf8 is induced by multiple autophagy-inducing stresses, including ifnγ and toll-like receptor stimulation, bacterial infection, starvation and by macrophage colony-stimulating factor. irf8 directly activates many genes involved in various steps of ... | 2015 | 25775030 |
| erratum to: lmo0171, a novel internalin-like protein, determines cell morphology of listeria monocytogenes and its ability to invade human cell lines. | | 2015 | 25773205 |
| characterisation of the microflora contaminating the wooden vats used for traditional sicilian cheese production. | traditional sicilian cheese productions are carried out employing traditional wooden vats, called tina. many studies have highlighted the beneficial role of wooden dairy equipment by contributing to enriching the milk microflora and improving the acidification processes. the present work was undertaken to evaluate the safety of the wooden vats used to coagulate milk. to this purpose, the different microbial populations hosted onto the internal surfaces of the vats used to produce two different s ... | 2015 | 27800376 |
| tea and soybean extracts in combination with milk fermentation inhibit growth and enterocyte adherence of selected foodborne pathogens. | this study examined the antibacterial and anti-adhesive properties of pure plant extracts (ppes) of green tea (gt), black tea (bt) and soybean individually or in combination with milk. fermented phenolic enriched-milk (fpem) was prepared by combining ppes with milk and fermented with lactic acid bacteria. antimicrobial activity of extracts was evaluated by broth-dilution and agar diffusion assay. anti-adhesive property of extracts was evaluated in caco-2 cell model. results from antibacterial te ... | 2015 | 25766833 |
| [the metabolism of the innate immunity cells in bacterial infections]. | metabolic activity of innate immunity cells infected by various doses of gram-negative (yersinia pseudotuberculosis, salmonella enteritidis) and gram-positive (staphylococcus aureus, listeria monocytogenes) bacteria has been investigated. using various animal models we found that during the initial period (up to 2 days) changes of infection in cellular responses depend on the type of the pathogen. in response to infection caused by gram-negative bacteria predominant neutrophil accumulation in th ... | 2015 | 25762604 |
| a novel therapy for melanoma developed in mice: transformation of melanoma into dendritic cells with listeria monocytogenes. | listeria monocytogenes is a gram-positive bacteria and human pathogen widely used in cancer immunotherapy because of its capacity to induce a specific cytotoxic t cell response in tumours. this bacterial pathogen strongly induces innate and specific immunity with the potential to overcome tumour induced tolerance and weak immunogenicity. here, we propose a listeria based vaccination for melanoma based in its tropism for these tumour cells and its ability to transform in vitro and in vivo melanom ... | 2015 | 25760947 |
| gas chromatography-mass spectrometry characterisation of the anti-listeria components of garcinia kola seeds. | adsorption chromatography was used to separate the bioactive constituents of the crude n-hexane extract of garcinia kola seeds. the silica gel 60 column fractions were eluted using the solvent combination of benzene: ethanol : ammonium hydroxide (bea) in the ratio combination of 36 : 4 : 0.4 v/v. the fractions were tested for anti-listeria activities by determining their mic50, mic90 or mic against 4 listeria isolates. the fractions were labelled bea1 to bea5 and 3 out of the 5 fractions eluted ... | 2015 | 25757343 |
| presence of listeria monocytogenes in mediterranean-style dry fermented sausages. | the morphological, physiological and epidemiological features of l. monocytogenes, together with the severity of human listeriosis infections, make l. monocytogenes of particular concern for manufacturers of cold-stored "ready to eat" (rte) foods. l. monocytogenes has been isolated from a wide variety of rte foods and is responsible for several outbreaks associated with the consumption of rte meat, poultry, dairy, fish and vegetable products. although l. monocytogenes is among the most frequentl ... | 2015 | 28231188 |
| antibiotic resistance of listeria monocytogenes isolated from meat-processing environments, beef products, and clinical cases in brazil. | the present study aimed to assess the antimicrobial resistance and the presence of virulence markers in 137 listeria monocytogenes isolates obtained from meat-processing environments, beef products, and clinical cases. all isolates were subject to molecular serogrouping and their antibiotic resistance profiles were assessed against 12 antimicrobials. in addition, isolates were subjected to detection of virulence marker genes (inla, inlc, inlj). the isolates were classified into serogroups 4b, 4d ... | 2015 | 25756759 |
| cxcr3 blockade protects against listeria monocytogenes infection-induced fetal wastage. | mammalian pregnancy requires protection against immunological rejection of the developing fetus bearing discordant paternal antigens. immune evasion in this developmental context entails silenced expression of chemoattractant proteins (chemokines), thereby preventing harmful immune cells from penetrating the maternal-fetal interface. here, we demonstrate that fetal wastage triggered by prenatal listeria monocytogenes infection is driven by placental recruitment of cxcl9-producing inflammatory ne ... | 2015 | 25751061 |
| novel inhibitor discovery and the conformational analysis of inhibitors of listeriolysin o via protein-ligand modeling. | increasing bacterial resistance to available antibiotics makes the discovery of novel efficacious antibacterial agents a priority. a previous report showed that listeriolysin o (llo) is a critical virulence factor and suggested that it is a target for developing anti-virulence drugs against listeria monocytogenes infections. in this study, we report the discovery of llo natural compound inhibitors with differential activity by using hemolysis assay. the mechanism of action of the inhibitors was ... | 2015 | 25749652 |
| rapid identification and source-tracking of listeria monocytogenes using maldi-tof mass spectrometry. | listeria monocytogenes is an important foodborne pathogen responsible for the sometimes fatal disease listeriosis. public health concerns and stringent regulations associated with the presence of this pathogen in food and food processing environments underline the need for rapid and reliable detection and subtyping techniques. in the current study, the application of matrix assisted laser desorption/ionisation-time-of-flight mass spectrometry (maldi-tof ms) as a single identification and source- ... | 2015 | 25747262 |
| label-free impedimetric detection of listeria monocytogenes based on poly-5-carboxy indole modified ssdna probe. | listeria monocytogenes is a life threatening pathogenic bacteria concerned with human health. the accurate and rapid detection of l. monocytogenes is required for preventing of listeriosis. in this study, dna sensing probe based on conducting polymer poly-5-carboxy indole (5c pin) was developed for the detection of l. monocytogenes hlya gene responsible for pathogenicity. the probe sequences (24 mer ssdna) were covalently immobilized on 5c pin via n-(3-dimethylaminopropyl)-n'-ethylcarbodiimidehy ... | 2015 | 25746904 |
| microbiological contamination of dried and lyophilized garlic as a potential source of food spoilage. | garlic is valued more for its flavoring and used in a wide variety of foods. in food technology, fresh garlic is not used, but instead its processed forms, most often dried and lyophilized, are utilized. the quality and safety of the final product largely depends on their microbiological quality. this research has provided information about effect of garlic fixation methods and provided information about effect of microbiological contamination of garlic used as a spice for quality of garlic mayo ... | 2015 | 25745260 |
| genome sequences of five disinfectant-resistant listeria monocytogenes strains from two iberian pork-processing plants. | we announce the draft genome sequences of five listeria monocytogenes strains from two iberian pork-processing plants located in spain that were distinguished by their resistance to benzalkonium chloride. these strains seem highly adapted to the meat-processing environment according to their persistence and transmission capabilities. | 2015 | 25745005 |
| whole-genome sequence of listeria monocytogenes serovar 4b strain izsam_lm_hs2008, isolated from a human infection in italy. | listeria monocytogenes is one of the most important foodborne pathogens. in this report, we present the complete and annotated genome of l. monocytogenes sequence type 06 (st06) serovar 4b strain izsam_lm_hs2008, isolated from an adult immunocompetent patient who developed the disease and died. | 2015 | 25744995 |
| listeria monocytogenes meningitis in an immunocompromised patient. | this report describes a case of meningitis caused by listeria monocytogenes in a stem cell transplant recipient on immunosuppressive therapy for cutaneous chronic graft-versus host disease. a 59-year-old woman had undergone allogeneic stem cell transplantation (from a matched unrelated donor) 13 months previously for chronic lymphocytic leukemia. she was on regular hematologic follow-up. though her previous malignancy has been in remission, she was immunosuppressed due to the pharmacological tre ... | 2015 | 25742155 |
| effect of chitosan on spoilage bacteria, escherichia coli and listeria monocytogenes in cured chicken meat. | the effects of essential oil (eo) of oregano and chitosan on the microbial quality and growth inhibition of listeria monocytogenes and escherichia coli o157:h7 in the iranian traditional ready-to-barbecue chicken was evaluated. thus, three groups of samples were prepared. one of them was considered to evaluate for aerobic plate count (apc), lactic acid bacteria (lab), psychrophilic and enterobacteriacae counts and the other two groups were inoculated with e. coli o157:h7 and l. monocytogenes 4b ... | 2015 | 25735728 |
| molecular cloning and characterization of autophagy-related gene tmatg8 in listeria-invaded hemocytes of tenebrio molitor. | macroautophagy (hereinafter called autophagy) is a highly regulated process used by eukaryotic cells to digest portions of the cytoplasm that remodels and recycles nutrients and disposes of unwanted cytoplasmic constituents. currently 36 autophagy-related genes (atg) and their homologs have been characterized in yeast and higher eukaryotes, including insects. in the present study, we identified and functionally characterized the immune function of an atg8 homolog in a coleopteran insect, tenebri ... | 2015 | 25727880 |
| outbreak investigation identifies a single listeria monocytogenes strain in sheep with different clinical manifestations, soil and water. | listeria (l.) monocytogenes causes orally acquired infections and is of major importance in ruminants. little is known about l. monocytogenes transmission between farm environment and ruminants. in order to determine potential sources of infection, we investigated the distribution of l. monocytogenes genetic subtypes in a sheep farm during a listeriosis outbreak by applying four subtyping methods (maldi-tof-ms, mlst, mlva and pfge). l. monocytogenes was isolated from a lamb with septicemia and f ... | 2015 | 25726302 |
| comparative proteomic analysis of listeria monocytogenes atcc 7644 exposed to a sublethal concentration of nisin. | listeria monocytogenes infections have been frequently reported in many food poisoning outbreaks around the world. in this work, the protein repertoires of l. monocytogenes atcc 7644 cells treated or not with a 10(-3)mg/ml nisin sublethal concentration, established by antimicrobial susceptibility tests, were analyzed by lc-ms/ms. overall, 179 proteins were identified, 9 of them more abundant in nisin-treated samples, and 4 more abundant in non-treated control samples. in nisin treated cells, pro ... | 2015 | 25724729 |
| previous physicochemical stress exposures influence subsequent resistance of escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes to ultraviolet-c in coconut liquid endosperm beverage. | this study investigated the influences of prior exposures to common physicochemical stresses encountered by microorganisms in food and food processing ecologies such as acidity, desiccation, and their combinations, on their subsequent susceptibility towards uv-c treatment in coconut liquid endosperm beverage. cocktails of escherichia coli o157:h7, salmonella enterica, and listeria monocytogenes were separately subjected to gradually acidifying environment (final ph 4.46), exposed to abrupt desic ... | 2015 | 25723813 |
| plasmid-borne cadmium resistant determinants are associated with the susceptibility of listeria monocytogenes to bacteriophage. | listeria monocytogenes is an intracellular pathogen causing gastroenteritis, central nervous system infections and abortions. chromosomal virulence determinants have been extensively investigated. however, the function of genes encoded by plasmids in l. monocytogenes has not been fully understood. in this study, we determined the prevalence and molecular profile of plasmids in food isolates of l. monocytogenes and examined the contribution of four plasmid-borne cadmium-resistant genes to the sus ... | 2015 | 25721472 |
| whole-genome sequences of 12 clinical strains of listeria monocytogenes. | listeria monocytogenes is a foodborne pathogen of global concern due to the high mortality rate among immunocompromised patients. whole-genome sequences of 12 strains of l. monocytogenes from humans were reported. the availability of these genomes should provide useful information on the evolutionary history and genetic diversity of l. monocytogenes. | 2015 | 25720683 |
| seek and destroy process: listeria monocytogenes process controls in the ready-to-eat meat and poultry industry. | the majority of human listeriosis cases appear to be caused by consumption of ready-to-eat (rte) foods contaminated at the time of consumption with high levels of listeria monocytogenes. although strategies to prevent growth of l. monocytogenes in rte products are critical for reducing the incidence of human listeriosis, control of postprocessing environmental contamination of rte meat and poultry products is an essential component of a comprehensive l. monocytogenes intervention and control pro ... | 2015 | 25710164 |
| assessing the survival of listeria monocytogenes in a domestic freezer by analyzing subsequent growth at 30°c using a novel reference method. | listeria monocytogenes is a serious pathogen capable of extensive survival under frozen storage. using optical density and multiple initial inocula in multiple identically prepared microtiter plates, the effect of storage time at -22°c on the subsequent growth at 30°c of the organism when defrosted was studied using a technique that compared the growth (through time to detection) of a test plate (previously frozen) with that of an identically prepared control plate, analyzed at the start of the ... | 2015 | 25710150 |
| survival of salmonella, escherichia coli o157:h7, and listeria monocytogenes on raw peanut and pecan kernels stored at -24, 4, and 22°c. | cocktails of lawn-collected cells were used to determine the survival of salmonella, escherichia coli o157:h7, and listeria monocytogenes on the surface of raw peanut and pecan kernels. kernels were inoculated with mixtures of four to five strains at 3 or 6 log cfu/g, dried at room temperature, and then stored at -24 ± 1, 4 ± 2, and 22 ± 1°c for 28 or 365 days. in most cases, rates of decline of the pathogens did not differ significantly between the two inoculum concentrations in the 28-day stud ... | 2015 | 25710147 |
| estimation of listeria monocytogenes and escherichia coli o157:h7 prevalence and levels in naturally contaminated rocket and cucumber samples by deterministic and stochastic approaches. | the aims of the present study were to determine the prevalence and levels of listeria monocytogenes and escherichia coli o157:h7 in rocket and cucumber samples by deterministic (estimation of a single value) and stochastic (estimation of a range of values) approaches. in parallel, the chromogenic media commonly used for the recovery of these microorganisms were evaluated and compared, and the efficiency of an enzyme-linked immunosorbent assay (elisa)-based protocol was validated. l. monocytogene ... | 2015 | 25710146 |
| manure source and age affect survival of zoonotic pathogens during aerobic composting at sublethal temperatures. | heat is the primary mechanism by which aerobic composting inactivates zoonotic bacterial pathogens residing within animal manures, but at sublethal temperatures, the time necessary to hold the compost materials to ensure pathogen inactivation is uncertain. to determine the influence of the type of nitrogen amendment on inactivation of salmonella, listeria monocytogenes, and escherichia coli o157:h7 in compost mixtures stored at sublethal temperatures, specific variables investigated in these stu ... | 2015 | 25710145 |
| receptor binding proteins of listeria monocytogenes bacteriophages a118 and p35 recognize serovar-specific teichoic acids. | adsorption of a bacteriophage to the host requires recognition of a cell wall-associated receptor by a receptor binding protein (rbp). this recognition is specific, and high affinity binding is essential for efficient virus attachment. the molecular details of phage adsorption to the gram-positive cell are poorly understood. we present the first description of receptor binding proteins and a tail tip structure for the siphovirus group infecting listeria monocytogenes. the host-range determining ... | 2015 | 25708539 |
| invasive listeria monocytogenes infection after liver transplantation: a life-threatening condition. | | 2015 | 25706469 |
| listeria monocytogenes meningitis in the elderly: distinctive characteristics of the clinical and laboratory presentation. | | 2015 | 25704282 |
| the clinical features, diagnosis, treatment, and prognosis of neuroinvasive listeriosis: a multinational study. | the aim of this study was to determine the independent risk factors, morbidity, and mortality of central nervous system (cns) infections caused by listeria monocytogenes. we retrospectively evaluated 100 episodes of neuroinvasive listeriosis in a multinational study in 21 tertiary care hospitals of turkey, france, and italy from 1990 to 2014. the mean age of the patients was 57 years (range, 19-92 years), and 64% were males. the all-cause immunosuppression rate was 54 % (54/100). forty-nine (49 ... | 2015 | 25698311 |
| molecular basis for the recognition of cyclic-di-amp by psta, a pii-like signal transduction protein. | cyclic-di-amp (c-di-amp) is a broadly conserved bacterial second messenger that is of importance in bacterial physiology. the molecular receptors mediating the cellular responses to the c-di-amp signal are just beginning to be discovered. psta is a previously uncharacterized pii -like protein which has been identified as a c-di-amp receptor. psta is widely distributed and conserved among gram-positive bacteria in the phylum firmicutes. here, we report the biochemical, structural, and functional ... | 2015 | 25693966 |
| interaction of graphene family materials with listeria monocytogenes and salmonella enterica. | graphene family materials have unique properties, which make them valuable for a range of applications. the antibacterial properties of graphene have been reported; however, findings have been contradictory. this study reports on the antimicrobial proprieties of three different graphene materials (pristine graphene (pg), graphene oxide (go), and reduced graphene oxide (rgo)) against the food-borne bacterial pathogens listeria monocytogenes and salmonella enterica. a high concentration (250 μg/ml ... | 2015 | 25685114 |
| ph-responsive deoxyribonucleic acid capture/release by polydopamine functionalized magnetic nanoparticles. | polydopamine functionalized magnetic nanoparticles (pda@fe3o4) were prepared and characterized by transmission electron microscopy, scanning electron microscopy, zeta potential and vibrating sample magnetometry. they were found to enable highly efficient capture of genomic deoxyribonucleic acid (dna). the adsorption capacity of pda@fe3o4 for genomic dna can reach 161 mg g(-1). the extraction protocol used aqueous solutions for dna binding to and releasing from the surface of the magnetic particl ... | 2015 | 25682426 |
| [septic vasculitis caused by listeria monocytogenes]. | septic vasculitis is a medium and small-vessel vasculitis caused by direct action of pathogens, associated with bacteremia. it is an uncommon condition; clinical manifestations include purpura, ulcers and vesicles-pustules. most cases of septic vasculitis are related to meningococcemia. there are no cases reported in medical literature associated to listeria spp. we report a case of a 71 year-old man who presented sepsis by listeria monocytogenes, and then evolved with purpuric skin lesions. ski ... | 2014 | 25679934 |
| hplc/ms analysis of polyphenols, antioxidant and antimicrobial activities of artabotrys hildebrandtii o. hffm. extracts. | the purpose of this work was to evaluate chemical constituents, antioxidant and antimicrobial activities of artabotrys hildebrandtii, an endemic medicinal plant from madagascar. ethanol extracts from the leaves and stem bark were tested to evaluate dpph free radical scavenging, using butylated hydroxytoluene and quercetin as standard antioxidants. an high-performance liquid chromatography/mass spectrometry method was developed to investigate the presence of phenolic compounds in the studied samp ... | 2015 | 25679267 |
| protective dendritic cell responses against listeriosis induced by the short form of the deubiquitinating enzyme cyld are inhibited by full-length cyld. | the deubiquitinating enzyme cyld is an important tumor suppressor and inhibitor of immune responses. in contrast to full-length cyld, the immunological function of the naturally occurring short splice variant of cyld (scyld) is insufficiently described. previously, we showed that dcs, which lack full-length cyld but express scyld, exhibit augmented nf-κb and dc activation. to explore the function of scyld in infection, we investigated whether dc-specific scyld regulates the pathogenesis of liste ... | 2015 | 25675948 |
| a case report of listeria monocytogenes meningoencephalitis in general hospital "dr radivoj simonovir" sombor. | listeria monocytogenes is one of the most common causes of bacterial central nervous system infections in adults. it often affects immunicompromised and elderly patients. even with appropriate antimicrobial treatment, mortality due to listeria monocytogenes meningoencephalitis is among the highest of all causes of bacterial central nervous system infections. | 2015 | 25675833 |
| nisin as a food preservative: part 1: physicochemical properties, antimicrobial activity, and main uses. | nisin is a natural preservative for many food products. this bacteriocin is mainly used in dairy and meat products. nisin inhibits pathogenic food borne bacteria such as listeria monocytogenes and many other gram-positive food spoilage microorganisms. nisin can be used alone or in combination with other preservatives or also with several physical treatments. this paper reviews physicochemical and biological properties of nisin, the main factors affecting its antimicrobial effectiveness, and its ... | 2016 | 25675115 |
| evaluation of transcription levels of inla, inlb, hly, bsh and prfa genes in listeria monocytogenes strains using quantitative reverse-transcription pcr and ability of invasion into human caco-2 cells. | listeria monocytogenes virulence depends on the activity of well-characterized virulence factors. in this study, transcription levels of inla, inlb, hly, bsh and prfa genes in l. monocytogenes strains, and the ability of invasion into caco-2 cells were investigated. serotyping, multiplex-pcr for serovar identification and restriction fragment analysis of inla were performed. transcription levels and invasiveness were evaluated by quantitative reverse-transcription pcr and by in vitro assays, res ... | 2015 | 25673285 |
| mir-182 is largely dispensable for adaptive immunity: lack of correlation between expression and function. | microrna (mir)-mediated regulation of protein abundance is a pervasive mechanism of directing cellular processes. the well-studied and abundant mir-182 has previously been implicated in many aspects of t cell function, dna repair, and cancer. in this study, we show that mir-182 is the most highly induced mir in b cells undergoing class-switch recombination. to elucidate the requirement of mir-182 in lymphocyte function, we extensively characterized mice with a targeted deletion of mir182. we sho ... | 2015 | 25672759 |
| organelle targeting during bacterial infection: insights from listeria. | listeria monocytogenes, a facultative intracellular bacterium responsible for severe foodborne infections, is now recognized as a multifaceted model in infection biology. comprehensive studies of the molecular and cellular basis of the infection have unraveled how the bacterium crosses the intestinal and feto-placental barriers, invades several cell types in which it multiplies and moves, and spreads from cell to cell. interestingly, although listeria does not actively invade host cell organelle ... | 2015 | 25670529 |
| listeria monocytogenes varies among strains to maintain intracellular ph homeostasis under stresses by different acids as analyzed by a high-throughput microplate-based fluorometry. | listeria monocytogenes, a food-borne pathogen, has the capacity to maintain intracellular ph (phi) homeostasis in acidic environments, but the underlying mechanisms remain elusive. here, we report a simple microplate-based fluorescent method to determine phi of listerial cells that were prelabeled with the fluorescent dye carboxyfluorescein diacetate n-succinimidyl ester and subjected to acid stress. we found that l. monocytogenes responds differently among strains toward organic and inorganic a ... | 2015 | 25667585 |
| the class ii phosphatidylinositol 3-phosphate kinase pik3c2a promotes shigella flexneri dissemination through formation of vacuole-like protrusions. | intracellular pathogens such as shigella flexneri and listeria monocytogenes achieve dissemination in the intestinal epithelium by displaying actin-based motility in the cytosol of infected cells. as they reach the cell periphery, motile bacteria form plasma membrane protrusions that resolve into vacuoles in adjacent cells, through a poorly understood mechanism. here, we report on the role of the class ii phosphatidylinositol 3-phosphate kinase pik3c2a in s. flexneri dissemination. time-lapse mi ... | 2015 | 25667265 |
| g0s2 modulates homeostatic proliferation of naïve cd8⁺ t cells and inhibits oxidative phosphorylation in mitochondria. | since its discovery, diverse functions have been attributed to the g0/g1 switch gene 2 (g0s2), from lipid metabolism to control of cell proliferation. our group showed for the first time that g0s2 promotes quiescence in hematopoietic stem cells by interacting with and retaining nucleolin around the nucleus. herein, we report the role of g0s2 in the differentiation and function of cd8(+) t cells examined in mice with an embryonic deletion of the g0s2 gene. g0s2 expression in naïve cd8(+) t cells ... | 2015 | 25666096 |
| efficacies of nisin a and nisin v semipurified preparations alone and in combination with plant essential oils for controlling listeria monocytogenes. | the food-borne pathogenic bacterium listeria is known for relatively low morbidity and high mortality rates, reaching up to 25 to 30%. listeria is a hardy organism, and its control in foods represents a significant challenge. many naturally occurring compounds, including the bacteriocin nisin and a number of plant essential oils, have been widely studied and are reported to be effective as antimicrobial agents against spoilage and pathogenic microorganisms. the aim of this study was to investiga ... | 2015 | 25662980 |
| listeria monocytogenes exopolysaccharide: origin, structure, biosynthetic machinery and c-di-gmp-dependent regulation. | elevated levels of the second messenger c-di-gmp activate biosynthesis of an unknown exopolysaccharide (eps) in the food-borne pathogen listeria monocytogenes. this eps strongly protects cells against disinfectants and desiccation, indicating its potential significance for listerial persistence in the environment and for food safety. we analyzed the potential phylogenetic origin of this eps, determined its complete structure, characterized genes involved in its biosynthesis and hydrolysis and id ... | 2015 | 25662512 |
| synergy among thymol, eugenol, berberine, cinnamaldehyde and streptomycin against planktonic and biofilm-associated food-borne pathogens. | essential oils have been found to exert antibacterial, antifungal, spasmolytic, and antiplasmodial activity and therapeutic effect in cancer treatment. in this study, the antibacterial activities of four main essential oils' components (thymol (thy), eugenol (eug), berberine (ber), and cinnamaldehyde (cin)) were evaluated against two food-borne pathogens, listeria monocytogenes and salmonella typhimurium, either alone or in combination with streptomycin. checkerboard assay demonstrated that thy ... | 2015 | 25661823 |
| a gold glyco-nanoparticle carrying a listeriolysin o peptide and formulated with advax™ delta inulin adjuvant induces robust t-cell protection against listeria infection. | in the search for an effective vaccine against the human pathogen, listeria monocytogenes (listeria), gold glyconanoparticles (gnp) loaded with a listeriolysin o peptide llo91-99 (gnp-llo) were used to immunise mice, initially using a dendritic cell (dc) vaccine approach, but subsequently using a standard parenteral immunisation approach. to enhance vaccine immunogenicity a novel polysaccharide adjuvant based on delta inulin (advax™) was also co-formulated with the gnp vaccine. confirming previo ... | 2015 | 25659269 |
| pfge standard operating procedures for listeria monocytogenes: harmonizing the typing of food and clinical strains in europe. | listeria monocytogenes is a foodborne pathogen responsible for a severe disease known as listeriosis. the european centre for disease prevention and control (ecdc) coordinates a network of national public health laboratories (nphls) in charge of typing clinical strains. in food, it is the european union reference laboratory for l. monocytogenes (eurl lm), which manages a network of national reference laboratories (nrls). a pulsed-field gel electrophoresis (pfge) standard operating procedure (eur ... | 2015 | 25658711 |
| whole-genome sequence of listeria monocytogenes strains from clinical and environmental samples from varanasi, india. | we present here the whole-genome sequences of listeria monocytogenes from ganges river water, agricultural soil, and human clinical samples from varanasi, india, which will be used for a comparative analysis. | 2015 | 25657276 |
| draft genome sequence of listeria monocytogenes lm201, isolated from foodstuff. | listeria monocytogenes is a facultative intracellular foodborne pathogen that can cause listeriosis in humans and animals. l. monocytogenes lm201 was isolated from foodstuff. the draft genome sequence of strain lm201 provides the genetic basis for the application of this strain in biotechnological vaccine production. | 2015 | 25657267 |
| characterization of polar lipids of listeria monocytogenes by hcd and low-energy cad linear ion-trap mass spectrometry with electrospray ionization. | listeria monocytogenes (l. monocytogenes) is a facultative, gram-positive, food-borne bacterium, which causes serious infections. although it is known that lipids play important roles in the survival of listeria, the detailed structures of these lipids have not been established. in this contribution, we described linear ion-trap multiple-stage mass spectrometric approaches with high-resolution mass spectrometry toward complete structural analysis including the identities of the fatty acid substi ... | 2015 | 25656850 |
| listeria monocytogenes as a cause of spontaneous bacterial peritonitis: a rare entity. | listeria is an uncommon cause of spontaneous bacterial peritonitis (sbp) in the united states. listeria should be suspected as a cause of sbp when the patient has diphtheria-like organisms on ascitic/blood cultures, iron overload/hemochromatosis, exposure to farm animals, or poor response to empiric therapy within 48-72 h. diagnosis of sbp is made if the ascitic fluid shows polymorphonuclear cell count >250 cells/mm(3) without an intra-abdominal source of infection. ampicillin with or without an ... | 2015 | 25656671 |
| microfluidic filtration and extraction of pathogens from food samples by hydrodynamic focusing and inertial lateral migration. | detecting pathogenic bacteria in food or other biological samples with lab-on-a-chip (loc) devices requires several sample preparation steps prior to analysis which commonly involves cleaning complex sample matrices of large debris. this often underestimated step is important to prevent these larger particles from clogging devices and to preserve initial concentrations when loc techniques are used to concentrate or isolate smaller target microorganisms for downstream analysis. in this context, w ... | 2015 | 25653055 |
| septicemic listeriosis in wild hares from saskatchewan, canada. | the bacterium listeria monocytogenes causes disease in a wide variety of mammals including rabbits and hares. we describe naturally acquired metritis and septicemic listeriosis in wild female hares from saskatchewan, canada. between april 2012 and july 2013, two white-tailed jackrabbits (lepus townsendii) and a snowshoe hare (lepus americanus) were presented to the veterinary medical centre at the western college of veterinary medicine, saskatoon, saskatchewan, canada with nonspecific neurologic ... | 2015 | 25647601 |
| contribution of the multiple type i signal peptidases to the secretome of listeria monocytogenes: deciphering their specificity for secreted exoproteins by exoproteomic analysis. | as commonly seen in monoderm bacteria, listeria monocytogenes possesses multiple membrane-bound signal peptidases of type i (spases i) called sipx, sipy and sipz. in order to decipher their respective contribution in an integrated and global view, the complement of the secretome corresponding to the exoproteome was resolved by two-dimensional gel electrophoresis (2-de). this was performed for l. monocytogenes sipx(-), sipy(-), sipz(-) single mutants, as well as for δsipxy and δsipyz double mutan ... | 2015 | 25637307 |
| src-dependent tyrosine phosphorylation of non-muscle myosin heavy chain-iia restricts listeria monocytogenes cellular infection. | bacterial pathogens often interfere with host tyrosine phosphorylation cascades to control host responses and cause infection. given the role of tyrosine phosphorylation events in different human infections and our previous results showing the activation of the tyrosine kinase src upon incubation of cells with listeria monocytogenes, we searched for novel host proteins undergoing tyrosine phosphorylation upon l. monocytogenes infection. we identify the heavy chain of the non-muscle myosin iia (n ... | 2015 | 25635050 |
| structure and mechanism of the caseinolytic protease clpp1/2 heterocomplex from listeria monocytogenes. | listeria monocytogenes is a devastating bacterial pathogen. its virulence and intracellular stress tolerance are supported by caseinolytic protease p (clpp), an enzyme that is conserved among bacteria. l. monocytogenes expresses two clpp isoforms that are only distantly related by sequence and differ in catalysis, oligomerization, active-site composition, and n-terminal interaction sites for associated aaa(+) chaperones. the crystal structure of the clpp1/2 heterocomplex from l. monocytogenes wa ... | 2015 | 25630955 |
| empirical antibiotic cover for listeria monocytogenes infection beyond the neonatal period: a time for change? | | 2015 | 25628458 |
| shelf life determination of sliced portuguese traditional blood sausage--morcela de arroz de monchique through microbiological challenge and consumer test. | morcela de arroz (ma) is a ready-to-eat blood and rice cooked sausage produced with pork, blood, rice, and seasonings, stuffed in natural casing and cooked above 90 °c/30 min. it is commercialized whole, not packed, with a restricted shelf life (1 wk/0 to 5 °c). the objective of this work was to establish sliced ma shelf life considering both the behavior of l. monocytogenes through a microbiological challenge test (mct) and the consumer acceptability of ma stored: vacuum packed (vp), modified a ... | 2015 | 25627176 |
| pi3-kinase activation is critical for host barrier permissiveness to listeria monocytogenes. | invasion of nonphagocytic cells, a critical property of listeria monocytogenes (lm) that enables it to cross host barriers, is mediated by the interaction of two bacterial surface proteins, inla and inlb, with their respective receptors e-cadherin and c-met. although inla-e-cadherin interaction is necessary and sufficient for lm crossing of the intestinal barrier, both inla and inlb are required for lm crossing of the placental barrier. the mechanisms underlying these differences are unknown. ph ... | 2015 | 25624443 |
| impact of the contamination level and the background flora on the growth of listeria monocytogenes in ready-to-eat diced poultry. | the food safety criteria that have been incorporated in european regulation (ec) no2073/2005 (official journal of the european union l, 338, 2005, 1), for listeria monocytogenes in ready-to eat (rte) foods, specify a maximum allowable concentration of 100 cfu g(-1) or ml(-1) . some factors such as ph, salt and modified atmosphere packaging (map) are used to prevent the growth of l. monocytogenes in order to comply with the limit. interactions between background microflora (bm) and l. monocytogen ... | 2015 | 25619748 |
| type i interferons in infectious disease. | type i interferons (ifns) have diverse effects on innate and adaptive immune cells during infection with viruses, bacteria, parasites and fungi, directly and/or indirectly through the induction of other mediators. type i ifns are important for host defence against viruses. however, recently, they have been shown to cause immunopathology in some acute viral infections, such as influenza virus infection. conversely, they can lead to immunosuppression during chronic viral infections, such as lympho ... | 2015 | 25614319 |
| risk-based control of food-borne pathogens listeria monocytogenes and salmonella enterica in the italian fermented sausages cacciatore and felino. | fermentation is the most important killing step during production of fermented meats to eliminate food-borne pathogens. the objective was to evaluate whether the food-borne pathogens listeria monocytogenes and salmonella enterica may survive during the production of two italian fermented sausages. sausage batter was inoculated with five strains of l. monocytogenes or s. enterica (ca. 10(5)-10(6) cfu/g) and their kinetic behavior was monitored during production. both pathogens survived relatively ... | 2015 | 25612557 |
| [listeriosis in mexico: clinical and epidemiological importance]. | listeriosis is caused by listeria monocytogenes, an important food-borne disease due to its clinical forms, high mortality rate, and the economic impact in both clinical and food production industries. in mexico, the lack of epidemiological surveillance systems leads to the need of accurate data on the incidence of listeriosis and its association with food-borne disease. in this paper, we present data about the presence of this bacterium in food, reports related to clinical cases of listeriosis, ... | 2017 | 25604418 |
| pregnancy related listeriosis: treatment and control. | listeriosis during pregnancy usually presents as an unremarkable febrile illness in the mother but can be fatal for the fetus and newborn. reliable laboratory testing for early diagnosis is lacking. serological antibody tests and bacteriological stool tests are not helpful since listeria-specific antibodies and stool cultures yielding the organism can be found in healthy pregnant women. because early diagnosis is difficult, diagnosis is usually made by culturing the pathogen from blood, cerebros ... | 2015 | 25604158 |
| proteome mining for drug target identification in listeria monocytogenes strain egd-e and structure-based virtual screening of a candidate drug target penicillin binding protein 4. | we used a combination of in-silico approaches to identify 168 promising drug targets in the proteome of a multidrug-resistant listeria monocytogenes strain; of these, one (pbp4) was particularly promising. virtual screening using it, followed by reverse docking, revealed four compounds namely nci524121, cap332797, nci524136 and zinc00518462 as good multi-target leads. | 2015 | 25601791 |