| phenolphthalein induces thymic lymphomas accompanied by loss of the p53 wild type allele in heterozygous p53-deficient (+/-) mice. | epidemiology studies have indicated that many human cancers are influenced by environmental factors. genetically altered mouse model systems offer us the opportunity to study the interaction of chemicals with genetic predisposition to cancer. using the heterozygous p53-deficient (+/-) mouse, an animal model carrying one wild type p53 gene and one p53 null allele, we studied the effects of phenolphthalein on tumor induction and p53 gene alterations. earlier studies showed that phenolphthalein cau ... | 1997 | 9437796 |
| neonatal early-onset escherichia coli disease. the effect of intrapartum ampicillin. | maternal intrapartum ampicillin has been recommended for the prevention of neonatal group b streptococcal disease. | 1998 | 9452705 |
| effects of nitric oxide synthase inhibition or sulfasalazine on the spontaneous colitis observed in hla-b27 transgenic rats. | the objective of this study was to determine the effects that certain nitric oxide synthase inhibitors have on the spontaneous intestinal and colonic inflammation that develops in hla-b27 transgenic rats and compare these data to those obtained using sulfasalazine (sz). in an attempt to more closely mimic the clinical situation, drug treatment was begun after the onset of colitis. hla-b27 male rats that developed clinical signs of colitis (diarrhea/loose stools) at 17 wk of age were randomized i ... | 1998 | 9454820 |
| characterization of campylobacter jejuni isolates from patients with guillain-barré syndrome. | campylobacter jejuni is a major pathogen preceding guillain-barré syndrome (gbs), and most c. jejuni isolates from gbs patients belong to penner serotype 19 (heat-stable; hs-19). we analyzed sixteen independent clinical isolates from gbs patients, twelve of which belonged to hs-19, three to hs-2, and one to hs-4, using pcr-based rflp analysis of a flagellin-a (flaa) gene. two isolates from patients with miller fisher syndrome (mfs), and 27 from patients with uncomplicated enteritis were also exa ... | 1997 | 9455985 |
| bystander effect-mediated therapy of experimental brain tumor by genetically engineered tumor cells. | transfer of the herpes simplex virus-thymidine kinase (hsv-tk) gene, followed by administration of ganciclovir (gcv), generates the "bystander effect," in which hsv-tk-negative wild-type cells, as well as hsv-tk-expressing cells, are killed by gcv. to eradicate an intracranial tumor by this bystander effect, we injected the tumor cells transduced with the hsv-tk gene (tk cells) in the vicinity of the preimplanted wild-type tumor and then administered gcv. wild-type 9l-gliosarcoma cells (1 x 10[5 ... | 1998 | 9458237 |
| uncoupling of muscarinic cholinergic phosphoinositide signals in senescent cerebral cortical and hippocampal membranes. | muscarinic-cholinergic signals in brain are mediated in part through the hydrolysis of phosphoinositides (ptdins) by phospholipase c (plc). to test the hypothesis that muscarinic ptdins signals change during aging, membranes were prepared from the cerebral cortex and hippocampus of young (4-6 months old), middle aged (8-10 months old) and senescent (24-26 months old) fisher 344 rats. carbachol dose-dependently increased [3h]-ptdins hydrolysis in both brain regions in all three age groups, howeve ... | 1998 | 9460709 |
| intracellular signal triggered by cholera toxin in saccharomyces boulardii and saccharomyces cerevisiae. | as is the case for saccharomyces boulardii, saccharomyces cerevisiae w303 protects fisher rats against cholera toxin (ct). the addition of glucose or dinitrophenol to cells of s. boulardii grown on a nonfermentable carbon source activated trehalase in a manner similar to that observed for s.cerevisiae. the addition of ct to the same cells also resulted in trehalase activation. experiments performed separately on the a and b subunits of ct showed that both are necessary for activation. similarly, ... | 1998 | 9464394 |
| long-term outcome of children following maternal human parvovirus b19 infection. | to determine the long-term outcomes of children exposed in utero to maternal parvovirus b19 infection. | 1998 | 9464735 |
| a role for mast cells in the development of adjuvant-induced vasculitis and arthritis. | the objective of this study was to characterize the role of mast cells in the development of vasculitis and joint swelling in adjuvant-immunized rats. leukocyte trafficking within mesenteric venules (rolling and adhesion) and mast cell activation (ruthenium red uptake) were examined in vivo. elevated leukocyte trafficking was observed by 4 days after immunization, whereas joint swelling developed between days 10 and 12. perivascular mast cells took up ruthenium red and appeared activated by elec ... | 1998 | 9466582 |
| effect of 2,4-dicholorophenoxyacetic acid, trifluralin and triallate herbicides on immune function. | the commercial formulations of 3 commonly used herbicides (the amine salt of 2,4-dichlorophenoxyacetic acid, trifluralin and triallate) were evaluated for effects on immune function in male fisher 344 rats. the herbicides were prepared in an olive oil vehicle and administered by oral gavage twice weekly for 28 d at the following doses: 10.0 mg 2,4-d/kg; 17.5 mg trifluralin/kg; 5.0 mg triallate/kg/treatment. normal body weight and organ/body weight ratios indicated the rats tolerated the herbicid ... | 1998 | 9467199 |
| efficacy of topical cyclosporine-loaded nanocapsules on keratoplasty rejection in the rat. | to evaluate the efficacy of a topical formulation of nanocapsules loaded with 1% cyclosporine a (csa), which has been previously demonstrated to enhance csa corneal penetration, compared to 1% csa in migliol oil on a penetrating keratoplasty rejection model in the rat. | 1998 | 9472469 |
| telomerase activation in nasopharyngeal carcinomas. | nasopharyngeal carcinomas (npc) are common in hong kong and southern china but rare in western countries. telomerase activation is common in human cancers but has not been reported previously in npc. telomerase activation in npc was determined using the sensitive trap (telomerase rapid amplification protocol) assay in 45 nasopharyngeal biopsies (36 npc, nine normal nasopharyngeal mucosae) in four xenografted npc tumours established in nude mice and in five in vitro npc cell lines. telomerase act ... | 1998 | 9472643 |
| effects of argon laser curing on dentin shear bond strengths. | previous studies have demonstrated the ability of the argon laser to polymerize light-activated materials and improve enamel shear bond strengths. this study was conducted to evaluate the effects of the argon laser on dentin shear bond strengths of current dentin bonding systems. argon laser (hgm model 8) at 231 and 280 mw, 5 sec bonding agent, 10 sec composite, and a conventional curing light (translux ec/kulzer) at 10 sec bonding agent, 20 sec composite were used to polymerize samples of denti ... | 1996 | 9484085 |
| relaxation of insulin-like growth factor-2 imprinting in rat cultured cells. | the parental-specific expression of the insulin-like growth factor-2 (igf-2) and h19 genes was studied in rat fibroblast cells derived from a 3 day-old first-generation hybrid animal obtained by crossing fisher and wistar strains (f x w cells). results showed that the reciprocal imprinting of the igf-2 and h19 genes was conserved in the rat tissues and in the derived f x w cells when cultured with frequent transfer. igf-2 and h19 gene expression was coordinately up-regulated upon reaching conflu ... | 1997 | 9484911 |
| isolation of a novel potassium channel gene hskca3 containing a polymorphic cag repeat: a candidate for schizophrenia and bipolar disorder? | many human hereditary neurodegenerative diseases are caused by expanded cag repeats, and anonymous cag expansions have also been described in schizophrenia and bipolar disorder. we have isolated and sequenced a novel human cdna encoding a neuronal, small conductance calcium-activated potassium channel (hskca3) that contains two arrays of cag trinucleotide repeats. the second cag repeat in hskca3 is highly polymorphic in control individuals, with alleles ranging in size from 12 to 28 repeats. the ... | 1998 | 9491810 |
| candesartan cilexetil reduces chronic renal allograft injury in fisher-->lewis rats. | to determine the effects of the angiotensin ii receptor antagonist, candesartan cilexetil, on glomerular and systemic blood pressures and the development of renal injury in lewis rat recipients of a single fisher kidney (f334--> lew transplantation), an established rat model of chronic renal allograft failure. | 1997 | 9493123 |
| differential behavior of the mo + pyf101 enhancer variant of moloney murine leukemia virus in rats and mice. | the mo + pyf101 enhancer variant of moloney murine leukemia virus (m-mulv) has been very useful in investigating m-mulv leukemogenesis. when inoculated subcutaneously (s.c.) into neonatal mice, mo + pyf101 m-mulv is attenuated for development of disease. previous studies in mice infected with wild-type m-mulv have revealed several important preleukemic events, including development of splenic hyperplasia, defects in bone marrow hematopoiesis, and in vivo generation of mcf viruses that arise by r ... | 1998 | 9501051 |
| effect of cholesterol content in diet on capillary flow of rat erythrocytes. part i: geometric and flow characteristics. | four different groups of male fisher rats were placed on one of the following diets for a period of one month: normal (control) diet, high cholesterol (hc) diet, high saturated fat (hf) diet and low fat (lf) diet. subsequently, blood samples were drawn and washed in phosphate buffered saline (pbs), and the red cells were resuspended in the same buffer. these samples were introduced into a polycarbonate capillary flow system and their flow characteristics observed. in addition, the mean cell volu ... | 1997 | 9502528 |
| effect of cholesterol content in diet on capillary flow of rat erythrocytes. part ii: mechanical properties. | erythrocytes from male fisher 344 rats on a high cholesterol diet (hc) exhibited a decreased ability to flow through capillaries when compared with those on a normal diet (control), a phenomenon which could not be explained by changes in geometric characteristics. to explain these results, changes in capillary-dependent rheological characteristics and intrinsic mechanical properties of red blood cells (rbc) due to diet were calculated. the hematocrit deficit in the capillary with respect to the ... | 1997 | 9502529 |
| multivariant analysis of men from infertile couples with and without antisperm antibodies. | research studies in animal and human systems have demonstrated conclusively that antisperm antibodies can interfere with fertilization. in the male, autoantibodies to sperm can be detected both in the sera and seminal plasma. | 1998 | 9506214 |
| interaction of hypoxia and aging in the heart: analysis of high energy phosphate content. | to test the hypotheses that aged myocardium has lower atp concentration and that a greater atp hydrolysis during hypoxia exaggerates diastolic dysfunction in aged myocardium, we used 31p nmr spectroscopy to measure atp and phosphocreatine (pcr) contents combined with heart function at baseline and during hypoxia and reoxygenation in perfused hearts isolated from young adult (3-4 months old) and old (24-25 months old) fisher 344 rats. at baseline, old hearts had 30% lower heart rate and prevalent ... | 1998 | 9515041 |
| rapid healing of venous ulcers and lack of clinical rejection with an allogeneic cultured human skin equivalent. human skin equivalent investigators group. | to test the safety, efficacy, and immunological impact of a cultured allogeneic human skin equivalent (hse) in the treatment of venous ulcers. | 1998 | 9521027 |
| the role of microglia and tumor-primed lymphocytes in the interaction between t lymphocytes and brain endothelial cells. | we investigated the role of ifn-gamma activated microglia in the passage of t lymphocytes across a monolayer of brain endothelial cells (ec) in vitro. microglia isolated from fisher 344 (f344) newborn rats were stimulated with ifn-gamma (100 u/ml) for 48 h. t lymphocytes primed with glioma cells were 51cr-labeled, and added to the monolayer of f344 brain ec. in the adhesion assay, when ec were cultured in medium containing the supernatant of reactive microglia before the assay was carried out, t ... | 1998 | 9521610 |
| the role of vitamin e in t-cell differentiation and the decrease of cellular immunity with aging. | the purpose of this study is to investigate the effects of vitamin e on both the decrease of cellular immunity with aging (section 2) and the differentiation of t-cells in thymus (section 3). in section 2, spontaneously hypertensive rats (shr) as a model for aging were used in this experiment and fed regular (50 iu/kg diet) or a high vitamin e (500 iu/kg diet) diet for 6 weeks. at 12 weeks old, they were killed and assayed. although proliferation of thymic lymphocytes was significantly decreased ... | 1998 | 9523031 |
| quantitation of age-related mitochondrial dna deletions in rat tissues shows that their pattern of accumulation differs from that of humans. | three age-related mtdna deletions were identified, and the competitive polymerase chain reaction (pcr) was used to quantitate their levels in different fisher 344 rat tissues. deletions that removed 4834; 13273; or 13415nt of the mitochondrial genome were shown to be associated with 16 (mtdna4834), nine (mtdna13273), or five (mtdna13415) nt direct repeats, respectively. the mtdna4834 deletion was detected in an age-related manner in all tissues screened; the mtdna13415 deletion was detected in o ... | 1998 | 9524209 |
| tgf-beta1 actions on frtl-5 cells provide a model for the physiological regulation of thyroid growth. | little is known about the tgf-beta1 mechanism that promotes thyroid cell growth arrest. we assessed tgf-beta1 effects on fisher rat thyroid cell line (frtl-5). this allowed us to study tgf-beta1 action on thyroid cells in various physiological situations such as actively proliferating cells, resting cells stimulated to proliferate by the action of various mitogens, and resting cells. tgf-beta1 arrested proliferating frtl-5 cells, increasing c-myc mrna levels and reducing p27-free cyclin d1 prote ... | 1998 | 9525744 |
| modulation of doxorubicin concentration by cyclosporin a in brain and testicular barrier tissues expressing p-glycoprotein in rats. | p-glycoprotein (pgp) is an inducible transmembrane protein that functions as an atp-dependent efflux pump. pgp is normally expressed in two types of cells: specialized epithelial cells with secretory/excretory functions (e.g., proximal renal tubules) and specialized endothelial cells (e.g., the capillary endothelial cells of the blood-brain barrier). in normal tissues, pgp could exert a cytoprotective effect by facilitating excretion of drugs. it follows that inhibition of pgp would alter the ph ... | 1998 | 9525837 |
| [epidemic neuropathy: proposal and arguments to rename strachan disease as strachan and madan disease]. | strachan's disease is a condition which mainly affects the nervous system. it is characterized by optic, auditory and peripheral neuropathies and lesions of the skin and mucous membranes. in 1955 miller fisher gave it this name, since the clinical condition described by henry strachan in jamaica during the nineteenth century was similar to that seen in canadian prisoners-of-war in japanese concentration camps during the second world war. | 1997 | 9528040 |
| the significance of epstein-barr virus seropositivity in multiple sclerosis patients? | the objective of this study was to evaluate and investigate the significance of the previously found 100% seropositivity toward epstein-barr virus (ebv) found in multiple sclerosis (ms) patients in contrast to healthy controls. using a commercially available elisa-test (biotest), which differentiates infections with ebv into previous infections, primary infections, reactivated infections and no previous infection, we found 137 of 138 ms patients and 124 of 138 healthy controls seropositive. a pr ... | 1998 | 9531433 |
| interferon-alpha treatment of chronic hepatitis c virus infection in children. | to determine the safety and efficacy of interferon-alpha therapy of chronic hepatitis c virus (hcv) infection in children. | 1998 | 9535253 |
| pancreatic bile-salt-dependent lipase activity in serum of diabetic patients: is there a relationship with glycation? | 1. pancreatic bile-salt-dependent lipase has been detected in human plasma where it has the capability to modify normal low- and high-density lipoprotein composition and structure and to reduce the atherogenicity of oxidized low-density lipoprotein (shamir r, johnson wj, morlock-fitzpatrick k, zolfaghari r, li l, mas e, lombardo d, morel dw, fisher ea. pancreatic carboxyl ester lipase: a circulating enzyme that modifies normal and oxidized lipoproteins in vitro. j clin invest 1996; 97: 1696-704) ... | 1998 | 9536927 |
| epididymal sperm aneuploidies in three strains of rats detected by multicolor fluorescence in situ hybridization. | a multicolor fluorescence in situ hybridization (fish) method was developed to detect aneuploidy and diploidy in epididymal sperm of rats using dna probes specific for chromosomes 4 and y. fourteen healthy young-adult rats from three strains were evaluated: inbred fisher 344/n/ehs, outbred sprague-dawley, and outbred wu wistar/cpb. the hybridization efficiency of the fish procedure was > 99.9%, the sex-ratio in sperm was approximately 1 as expected, and there was no significant variation among t ... | 1998 | 9544190 |
| spreading depression induces depletion of map2 in area ca3 of the hippocampus in a rat unilateral carotid artery occlusion model. | traumatic brain injury (tbi) induces neuronal cell loss in area ca3 of the hippocampus. however, it has not yet been established why traumatic injury of the cortex induces neuronal damage in more remote areas. spreading depression (sd) may be one potential mechanism for this pathophysiology. the present study evaluated whether sd on the cortex evokes a pathological change in the hippocampus. forty-two fisher rats were assigned to four groups: group i: sham operation (n = 7), group ii: right caro ... | 1998 | 9555973 |
| the effect of mao-a inhibition and cold-immobilization stress on n-acetylserotonin and melatonin in shr and wky rats. | selective monoamine oxidase (mao) a inhibitors and cold-immobilization stress (which increases the production of the endogenous mao-a inhibitor, tribulin) stimulate rat pineal melatonin biosynthesis in sprague-dawley and fisher 344n rats. considering the hyperactive sympathetic response of the hypertensive rats, it was interesting to compare the effect of clorgyline and cold-immobilization stress on pineal melatonin and related indoles levels in shr and wky rats (hplc-fluorimetric method). clorg ... | 1998 | 9564635 |
| effect of pentachlorophenol on immune function. | the organochlorine compound, pentachlorophenol, was evaluated for effects on immune system function in male fisher 344 rats. pentachlorophenol was prepared in an olive oil vehicle and was administered by oral gavage twice weekly for 28 days at a dose of 2.0 mg/kg per treatment. exposure to pentachlorophenol increased body weight gains (p=0.024) during the treatment period. liver (p=0.034) and kidney (p=0.012) body weight ratios were also increased. pentachlorophenol exposure enhanced t-lymphocyt ... | 1998 | 9570329 |
| developmental expression of cytochrome p450 isoforms after transplantation of fetal liver tissue suspension into the spleens of adult syngenic rats. | in the present study, the developmental expression of three cytochrome p450 (p450) isoforms, 1a1, 2b1 and 3a2, and the ability to store glycogen was investigated in intrasplenic liver cell explants in comparison to adult and fetal liver. fetal liver tissue suspensions were transplanted into the spleens of adult male syngenic fisher inbred rats. animals were sacrificed at 3 days, 1, 2, 4 weeks, 2, 4, 6 months and 1 year after transplantation. spleens and livers of transplant recipients were compa ... | 1998 | 9570501 |
| predictive neural networks for learning the time course of blood glucose levels from the complex interaction of counterregulatory hormones. | diabetes mellitus is a widespread disease associated with an impaired hormonal regulation of normal blood glucose levels. patients with insulin-dependent diabetes mellitus (iddm) who practice conventional insulin therapy are at risk of developing hypoglycemia (low levels of blood glucose), which can lead to severe dysfunction of the central nervous system. in large retrospective studies, up to approximately 4% of deaths of patients with iddm have been attributed to hypoglycemia (cryer, fisher, & ... | 1998 | 9573413 |
| the response of dunning r3327 prostatic adenocarcinoma to il-2, histamine and radiation. | a syngeneic, androgen-sensitive dunning r3327 rat prostatic adenocarcinoma was transplanted bilaterally in the flanks of male copenhagen fisher rats. approximately 3 months after implantation, when the tumours had a median volume of 150 mm3, one group of rats was treated with histamine alone (4 mg kg(-1) subcutaneously on week days), another group with human recombinant interleukin 2 (il-2) alone (425 iu kg(-1) continuous infusion) and a third group with both histamine and il-2 during 6 weeks. t ... | 1998 | 9579825 |
| lipo-oligosaccharides of campylobacter jejuni serotype o:10. structures of core oligosaccharide regions from a bacterial isolate from a patient with the miller-fisher syndrome and from the serotype reference strain. | lipo-oligosaccharide (losa) was obtained by phenol-water extraction of bacterial cells of an isolate pg 836, identified as campylobacter jejuni serotype o:10, from a patient who subsequently developed the miller-fisher syndrome (mfs). the product was separated into a water-insoluble gel of low mr and a water-soluble component of high mr. the structure of the core oligosaccharide region in losa is reported herein for comparison with losb from the c. jejuni o:10 reference strain, and is based on i ... | 1997 | 9581276 |
| antibodies to gt1a ganglioside in patients with guillain-barré syndrome. | serum antibodies from 8 (13%) of 62 patients with the acute guillain-barré syndrome (gbs) and 1 of 3 patients with the miller fisher syndrome (mfs) recognized a minor ganglioside in bovine and human brain trisialoganglioside fractions. the ganglioside antigen migrated between gd1a and gd1b on thin-layer chromatograms. the structure of this ganglioside was established to be gt1a by thin-layer chromatography blotting and mass spectrometry. gt1a a ganglioside was also detected in human and bovine p ... | 1998 | 9585812 |
| immobilization effects on contractile properties of aging rat skeletal muscle. | the effect of four weeks of ankle immobilization on muscle mass and in situ contractile properties of soleus (sol), plantaris (pla), and extensor digitorum longus (edl) of 31- and 37-month-old (mo) fisher 344/brown norway rats was examined. following contractile tests, muscles were harvested, weighed, and analyzed for fiber type and fiber cross-sectional area. body weights (g) were similar in both control (con) groups (31 mo, 578 +/- 20; 37 mo, 553 +/- 26; mean +/- se) and both immobilized (im) ... | 1998 | 9589753 |
| the long-term clinical outcome of elderly patients with helicobacter pylori-associated peptic ulcer disease. | the objective of this study was to evaluate the long-term efficacy of helicobacter pylori (hp) eradication treatment in elderly patients with hp-associated peptic ulcer. a total of 56 subjects, 25 affected with gastric ulcer (gu, 9 males, 16 females, mean age 77.8 years, range 67-93) and 31 with duodenal ulcer (du, 19 males, 12 females, mean age 76.5 years, range 65-87) were confirmed to be hp-positive by gastric histology and the rapid urease test. all patients were then consecutively treated w ... | 1998 | 9592687 |
| [relationship between transcription and repair of radioinduced dna damage]. | the dna repair is occurred on genome level with different rates. this rates are depend on a transcription activity of various genome sequences. the repair rates are low in non-transcribed genes and dna sequences, more higher in transcribed genes and the highest in actively transcribed strands of active genes. such classification could be applied to the repair of uv-induced cyclobutane pyrimidine dimers and for other (but not for all) dna lesions. this observation has become a basement of such im ... | 1997 | 9599610 |
| modulation by 5-ht1a receptors of the 5-ht2 receptor-mediated tachykinin-induced contraction of the rat trachea in vitro. | 1. in the fisher 344 rat, tachykinins have been shown to cause the release of 5-hydroxytryptamine (5-ht) from airway mast cells, which then causes direct smooth muscle activation as well as the release of acetylcholine from cholinergic nerves. the aim of the present study was to examine the modulatory effects of 5-ht receptors on the neurokinin a (nka)-induced release of endogenous 5-ht and airway smooth muscle contraction in the isolated fisher 344 rat trachea. 2. the selective 5-ht2 receptor a ... | 1998 | 9605563 |
| perfusion quantitation in transplanted rat kidney by mri with arterial spin labeling. | the purpose of this study was to determine the feasibility of using quantitative magnetic resonance imaging (mri) with non-invasive arterial spin labeling to assess perfusion of transplanted kidneys in rats. mri studies were performed on five groups of rats: normal fisher 344 rats, fisher 344 rats that had received a syngeneic kidney transplant either 3 or seven days prior to study, and fisher 344 rats that had received an allogeneic kidney (aci rat as the donor) either three or seven days prior ... | 1998 | 9607213 |
| spontaneous proliferative lesions in the nasopharyngeal meatus of f344 rats. | spontaneous proliferative lesions in the nasopharyngeal meatus were identified as the cause of death in 12 of 1,600 male and 5 of 1,600 female fisher 344 (f344) rats used in 2-yr carcinogenicity studies; none of the lesions were considered treatment related. all the rats showed dyspnea, abdominal distension, and clinical deterioration. gross features were characterized by simultaneous occurrence of conspicuous gaseous distension of the intestinal tract, especially in the ileum and cecum, and foc ... | 1998 | 9608649 |
| cloning and expression of rat lung acidic ca(2+)-independent pla2 and its organ distribution. | a clone for a rat acidic ca(2+)-independent phospholipase a2 (aipla2) was isolated from a cdna library prepared from rat granular pneumocytes with a probe based on the human aipla2 sequence (t.s. kim, c.s. sundaresh, s. i. feinstein, c. dodia, w. r. skach, m. k. jain, t. nagase, n. seki, k. ishikawa, n. nomura, and a. b. fisher. j. biol. chem. 272: 2542-2550, 1997). in addition, a consensus sequence for mouse aipla2 was constructed from several mouse cdna clones in the genbank and dbest database ... | 1998 | 9612290 |
| presence and possible role of vascular endothelial growth factor in thyroid cell growth and function. | angiogenesis is an important component in the development of thyroid goitre. vascular endothelial growth factor (vegf) represents a family of specific endothelial cell mitogens involved in normal angiogenesis and in tumour development. the purpose of this study was to determine the distribution of vegf in thyroid tissues during goitre formation, and to study the actions of vegf on the regulation of thymidine incorporation and iodine uptake by thyroid follicular cells. goitre was induced in adult ... | 1998 | 9614352 |
| hyperbaric oxygen in the treatment of acute muscle stretch injuries. results in an animal model. | hyperbaric oxygen therapy is an established therapy in several areas of clinical medicine. however, studies have produced conflicting results regarding its efficacy for sports-related soft tissue injuries. this study examines the use of hyperbaric oxygen therapy after an acute muscle stretch injury in an animal model. two groups of rabbits (nine in each group) were subjected to a partial stretch injury to the tibialis anterior muscle-tendon unit. for all 18 animals, the injuries were induced in ... | 1998 | 9617397 |
| functional transitions in myosin: formation of a critical salt-bridge and transmission of effect to the sensitive tryptophan. | for analyzing the mechanism of energy transduction in the "motor" protein, myosin, it is opportune both to model the structural change in the hydrolytic transition, atp (myosin-bound) + h2o --> adp.pi (myosin-bound) and to check the plausibility of the model by appropriate site-directed mutations in the functional system. here, we made a series of mutations to investigate the role of the salt-bridge between glu-470 and arg-247 (of chicken smooth muscle myosin) that has been inferred from crystal ... | 1998 | 9618467 |
| time and dose dependency of the suppression of pulmonary metastases of rat mammary cancer by amiloride. | amiloride is an inhibitor of urokinase plasminogen activator (upa), an essential component of the plasminogen/plasmin enzyme system. inhibition of upa prevents the conversion of plasminogen to tumor cell surface bound plasmin which is required for initiation of the metastatic process. matb rat mammary cancer cells were introduced into the jugular venous system of 80 fisher 344 female rats. amiloride at high and low dosages was administered in the drinking water at the time of, prior to or severa ... | 1998 | 9626814 |
| effects of macrophage migration inhibitory factor and macrophage migration stimulatory factor on function and survival of foetal dopaminergic grafts in the 6-hydroxydopamine rat model of parkinson's disease. | activated microglia play an important role in the rejection of intracerebral grafts and the degeneration of axotomized neurones. we studied the effect of macrophage migration stimulatory factor (msf) or macrophage migration inhibitory factor (mif) on allogeneic foetal mesencephalic dopaminergic grafts transplanted into the striatum of 6-hydroxydopamine-lesioned rats. rotation testing revealed a significant compensation of lesion-induced motor asymmetry 3 weeks post-grafting in animals treated wi ... | 1998 | 9628407 |
| neuroimmunomodulation in inflammatory bowel disease. how far from "bench" to "bedside"? | the chronic inflammatory bowel diseases (bid), crohn's disease and ulcerative colitis, are characterized by recurrent periods of inflammation and tissue destruction. the clinical course is influenced by genetics, environmental factors, and the immune system. recent insights (bench trials) benefiting from advances in genetic engineering and molecular biology have contributed to clinical care (bedside) in terms of actual or potential therapies. does the neuroendocrine system significantly modify d ... | 1998 | 9629299 |
| the fiber-type-specific effect of inactivity and intermittent weight-bearing on the gastrocnemius muscle of 30-month-old rats. | to characterize single skeletal muscle fiber contractile properties from the gastrocnemius muscle that occur during inactivity and intermittent weight-bearing in 30-month-old animals. | 1998 | 9630145 |
| optimal ppi-based triple therapy for the cure of helicobacter pylori infection: a single center comparison of four 14-day schedules. | helicobacter pylori eradication is accomplished using a wide array of drugs combined in a multitude of dosage schedules. the aim of the present study was to define the best 14-day eradication schedule using a ppi plus either two antibiotics or one antibiotic and bismuth. | 1998 | 9631310 |
| manganese-metalloporphyrin (atn-10) as a tumor-localizing agent: magnetic resonance imaging and inductively coupled plasma atomic emission spectroscopy study with experimental brain tumors. | we examined whether selective tumor accumulation of a novel manganese-metalloporphyrin (atn-10) occurs in fisher rats bearing intracerebral 9l gliomas. | 1998 | 9632193 |
| chronic ethanol consumption impairs spatial remote memory in rats but does not affect cortical cholinergic parameters. | we have studied learning, memory and cortical cholinergic parameters after oral administration of 20% v/v ethanol solution to male fisher rats for 6 months. a group of rats were trained to behave efficiently in an eight-arm radial maze and after that split into two subgroups submitted to ethanol or control treatment. ethanol-treated rats had more difficulty in relearning the same task 1 year later, compared to ethanol-untreated rats (control). differences in working memory performance were found ... | 1998 | 9632211 |
| alterations of antioxidant enzymes and oxidative damage to macromolecules in different organs of rats during aging. | oxygen free radicals have been hypothesized to play an important role in the aging process. to investigate the correlation between the oxidative stress and aging, we have determined the levels of oxidative protein damage and lipid peroxidation in the brain and liver, and activities of antioxidant enzymes in the brain, liver, heart, kidney, and serum from the fisher 344 rats at ages of 1, 6, 12, 18, and 24 months. the results showed that the level of oxidative protein damage (measured as carbonyl ... | 1998 | 9641266 |
| reduction of gut hypoplasia and cachexia in tumor-bearing rats maintained on total parenteral nutrition and treated with peptide yy and clenbuterol. | prevention of gut hypoplasia associated with total parenteral nutrition (tpn) was investigated in 67 adult male fisher 344 rats. mass and protein content of the small intestine was reduced by 31% and 39%, respectively, after 7 d of tpn in tumor-bearing (tb) rats. coinfusing peptide yy (pyy; 1 nmol.kg-1.h-1) and treating the rats with the anabolic beta-adrenergic agonist, clenbuterol (cle; 2 mg.kg-1.d-1), resulted in significant savings in small intestine weight (26% increase) and protein (42% in ... | 1998 | 9646290 |
| amniotic fluid complement c3 as a marker of intra-amniotic infection. | to determine the value of amniotic fluid (af) complement c3 as a marker of intra-amniotic infection and to compare complement c3 with other rapid markers of intra-amniotic infection. | 1998 | 9649097 |
| hysterosalpingography with videofluoroscopy: effect on radiologic practice in an academic medical center. | the authors attempted to determine whether videotaping the fluoroscopic portion of hysterosalpingography would result in changed diagnoses or an increase in diagnostic confidence. | 1998 | 9653464 |
| clinical comparison of bioabsorbable barriers with non-resorbable barriers in guided tissue regeneration in the treatment of human intrabony defects. | the purpose of the study was to compare the effects of guided tissue regeneration (gtr) with expanded polytetrafluoroethylene (eptfe) non-resorbable barriers and polylactic acid bioabsorbable barriers in humans with intrabony defects due to periodontitis. ten patients presented with 2 intrabony defects each. mucoperiosteal flaps were performed. one of the defects was randomly assigned for placement of the eptfe barrier over the roots and alveolar bone and the other defect with placement of the p ... | 1998 | 9660331 |
| glutamine enhances gut glutathione production. | the gastrointestinal tract is recognized as having important metabolic functions. this study examined gut glutathione (gsh) extraction and the effect of supplemental oral glutamine (gln) on gut gsh fractional release. | 1998 | 9661123 |
| fisher syndrome associated with igg anti-gq1b antibody following infection by a specific serotype of campylobacter jejuni. | the purpose of the study was to describe clinical and serologic features of fisher syndrome associated with igg anti-gq1b ganglioside antibody following campylobacter jejuni enteritis. | 1998 | 9663234 |
| physiologically-based pharmacokinetic model for trichloroethylene considering enterohepatic recirculation of major metabolites. | trichloroacetic acid (tca) is a major metabolite of trichloroethylene (tri) thought to contribute to its hepatocarcinogenic effects in mice. recent studies have shown that peak blood concentrations of tca in rats do not occur until approximately 12 hours following an oral dose of tri. however, blood concentrations of tri reach a maximum within an hour and are nondetectable after 2 hours. the results of a study which examined the enterohepatic recirculation (ehc) of the principle tri metabolites ... | 1998 | 9664722 |
| epidemiology and molecular identification of salmonella infections in children. | to explore the role of foods and the environment in the development of infections with salmonella in infants and children. | 1998 | 9667537 |
| antiglycosphingolipid immune responses in neurology. the vienna experience with isotypes, subclasses, and disease. | igm, igg, iga, and igg subclass anti-gm1, anti-gq1b, and anti-asialo-gm1 (anti-ga1) antibodies, respectively, were investigated by elisa in serum from neurological and other patients. increased anti-gm1 occurred mostly in approximately 15-35% of the cases without statistical differences; high percentages were found in guillain-barré syndrome (gbs) preceded by gastrointestinal infection and multifocal motor neuropathy. roughly, igm anti-gm1 was most frequent; however, distinct igg and iga reactio ... | 1998 | 9668368 |
| a chimeric influenza virus expressing an epitope of outer membrane protein f of pseudomonas aeruginosa affords protection against challenge with p. aeruginosa in a murine model of chronic pulmonary infection. | the ability of a chimeric influenza virus containing, within the antigenic b site of its hemagglutinin, an 11-amino-acid (aegrainrrve) insert from the peptide 10 epitope of outer membrane (om) protein f of pseudomonas aeruginosa to serve as a protective vaccine against p. aeruginosa was tested by using the murine chronic pulmonary infection model. mice immunized with the chimeric virus developed antibodies that reacted in an enzyme-linked immunosorbent assay with peptide 10, with purified protei ... | 1998 | 9673294 |
| neurosyphilis mimicking miller-fisher syndrome: a case report and mri findings. | a 36-year-old man presented with rapidly progressive unsteadiness of gait and diplopia. he had horizontal nystagmus, left sixth nerve palsy, severe ataxia, and areflexia. csf protein content was 2.4 g/l. despite a pleocytosis, miller-fisher syndrome was diagnosed. two years later, reevaluation revealed active neurosyphilis in the freshly drawn and stored serum and csf samples. clinical findings were consistent with tabes dorsalis. mri showed lesions confined to the posterior columns of the spina ... | 1998 | 9674818 |
| 4-aminobutyrate (gaba) transporters from the amine-polyamine-choline superfamily: substrate specificity and ligand recognition profile of the 4-aminobutyrate permease from bacillus subtilis. | a previous study [ferson, wray and fisher (1996) mol. microbiol. 22, 693-701] has shown that transposon-mediated disruption of a protein 47% identical to the escherichia coli gaba (4-aminobutyrate) transporter abolishes the ability of nitrogen-limited culture conditions to induce expression of a gaba transport activity in bacillus subtilis. here it is demonstrated directly that the b. subtilis gaba permease (gabp) gene can complement the transport defect in the gabp-negative e. coli strain. unex ... | 1998 | 9677314 |
| transplantation of fetal liver tissue suspension into the spleens of adult syngenic rats: effects of beta-naphthoflavone, phenobarbital and dexamethasone on cytochrome p450 isoforms expression and on glycogen storage. | in the present study, the effect of beta-naphthoflavone (bnf), phenobarbital (pb) and dexamethasone (dex) on the expression of three cytochrome p450 (p450) isoforms, 1a1, 2b1 and 3a2, and on glycogen storage was investigated in intrasplenic liver cell explants in comparison to adult liver. fetal liver tissue suspensions were transplanted into the spleens of adult male syngenic fisher inbred rats. four months after surgery, transplant recipients and age matched controls were orally treated with b ... | 1998 | 9681647 |
| developmental changes of cytochrome p450 dependent monooxygenase functions after transplantation of fetal liver tissue suspension into spleens of adult syngenic rats. | fetal liver tissue suspensions were transplanted into the spleens of adult male syngenic fisher 344 inbred rats. animals were sacrificed at 3 days, 1, 2, 4 weeks, and 2, 4 and 6 months after transplantation and cytochrome p450 (p450) dependent monooxygenase functions in spleen and liver 9000 g supernatants were assessed by measuring three model reactions for different p450 subtypes: ethoxyresorufin o-deethylation (erod; mainly 1a), ethoxycoumarin o-deethylation (ecod; predominantly 1a, 2a, 2b) a ... | 1998 | 9681655 |
| comparison of soleus muscles from rats exposed to microgravity for 10 versus 14 days. | the effects of two different duration space-flights on the extent of atrophy, fiber type composition, and myosin heavy chain (mhc) content of rat soleus muscles were compared. adult male fisher rats (n=12) were aboard flight sts-57 and exposed to 10 days of microgravity and adult ovariectomized female spraque-dawley rats (n=12) were aboard flight sts-62 for 14 days. soleus muscles were bilaterally removed from the flight and control animals and frozen for subsequent analyses. muscle wet weights, ... | 1998 | 9681692 |
| food restriction alters the age-related decline in cardiac beta-adrenergic responsiveness. | the responsiveness of the heart to beta-adrenergic receptor stimulation declines with age. the aim of this study was to determine whether food restriction (fr), the most effective means of retarding the aging processes, affects the loss of beta-adrenergic responsiveness. male fisher 344 rats, fed either ad libitum (group a) or allowed to eat only 60% of what group a rats consumed (group b), were sacrificed at 4, 11 or 22-28 months of age. the hearts were isolated and perfused via the langendorff ... | 1998 | 9681875 |
| endogenous marinobufagenin-like factor in acute plasma volume expansion. | the aim of the study was to further investigate the nature of endogenous digitalis-like factors stimulated by acute plasma volume expansion (ve). 10 male fisher 344xnb rats were given intravenous (iv) saline infusion (4% of the body mass) for 30 min, which caused a fall in % hematocrit (35.9+/-0.69 vs. 41.8+/-1.05; vs. 10 controls, p<0.01). edlf was measured in c-18 reverse-phase extracted (32%+80% acetonitrile) tissues. ve was associated with an increase in plasma marinobufagenin-like factor (m ... | 1998 | 9682914 |
| plasma marinobufagenin-like and ouabain-like immunoreactivity in adrenocorticotropin-treated rats. | recently, an endogenous digitalis-like factor (edlf) was shown to be stimulated in corticotropin (acth) hypertension in the rat. we have shown that mammalian plasma contains a vasoconstrictor na,k-atpase inhibitor, which cross-reacts with an antibody to amphibian edlf, marinobufagenin. in the present experiment, the effect of 8 days of intramuscular acth treatment (0.5 mg/kg/day) of male fisher 344 x nb rats on blood pressure, plasma ouabain-like and marinobufagenin-like immunoreactivity, and on ... | 1998 | 9683040 |
| subzero nonfreezing preservation in a murine limb replantation model. | this study was designed to examine the most effective temperature for hypothermic storage, without freezing, to prolong ischemic tolerance in an amputated murine hindlimb model. we measured freezing points in the calf muscle and the subcutaneous tissue of the foot in the amputated limbs of fisher 344 strain male inbred rats. the highest freezing point was -1.5 degrees c, which was recorded in the calf muscle. to prevent freezing in any of the tissues in the amputated limb, the temperature for th ... | 1998 | 9683769 |
| differential effect of 2-aminoethyl-isothiourea, an inhibitor of the inducible nitric oxide synthase, on microvascular blood flow and organ injury in models of hepatic ischemia-reperfusion and endotoxemia. | the vasodilator nitric oxide (no) is involved in the regulation of systemic blood pressure and local organ blood flow. inhibitors of the constitutively expressed nitric oxide synthase in endothelial cells (enos), e.g., nomega-nitro-l-arginine methyl ester hydrochloride (l-name), aggravated liver injury in a variety of models. on the other hand, inhibitors of the inducible nos (inos), e.g., 2-aminoethyl-isothiourea (aet), were found to be beneficial during endotoxemia. the aim of this investigati ... | 1998 | 9688086 |
| strain differences in cytochrome p4501a1 gene expression caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin in the rat liver: role of the aryl hydrocarbon receptor and its nuclear translocator. | rat strain variation in hepatic cytochrome p4501a1 (cyp1a1) gene expression caused by 2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) was investigated along with possible underlying mechanism. tcdd at a single oral dose of 13.5 ng/kg body weight significantly increased hepatic cyp1a1 mrna expression in drh, long-evans cinamon (lec), long-evans (le), and holtzman (ho) rats, but not in sprague-dawley (sd), wistar-imamichi (wi), lewis (lew), and fisher-344 (f344) strains. all showed significant inductio ... | 1998 | 9703964 |
| biopersistence of synthetic vitreous fibers and amosite asbestos in the rat lung following inhalation. | fiber biopersistence as a major mechanism of fiber-induced pathogenicity was investigated. the lung biopersistence of 5 synthetic vitreous fibers (svfs) and amosite asbestos was evaluated using the rat inhalation model. in contrast to several previous studies, this study examined fibers that dissolve relatively slowly in vitro at ph 7.4. fisher rats were exposed for 5 days by nose-only inhalation to refractory ceramic fiber (rcf1a), rock (stone) wool (mmvf21), 2 relatively durable special applic ... | 1998 | 9707503 |
| [a study of soluble form of selectin family in the sera of patients with neuroimmunological disorders]. | members of the selectin family mediate the first step of leukocyte-endothelial cell interaction in inflammatory lesions. soluble(s) l-selectin, se-selectin and sp-selectin were examined by monoclonal antibody-based enzyme linked immunosorbent assay on serum samples taken from patients with neuroimmunological disorders, namely, 35 cases of multiple sclerosis (ms), 18 of guillain-barré syndrome (gbs), 7 of miller-fisher syndrome (mfs), 8 of chronic inflammatory demyelinating polyneuropathy (cidp), ... | 1998 | 9711113 |
| fetal growth retardation, particularly within lymphoid organs, following repeated maternal injections of betamethasone in sheep. | to examine the effect of single or repeated injections of maternally administered corticosteroids on fetal growth in sheep. | 1998 | 9714987 |
| regionally different influence of contractile agonists on isolated rat airway segments. | rats are increasingly used to study airway inflammation and bronchial responsiveness. relative little is known on the contribution of small airways in this animal model. we therefore compared the responsiveness to various inflammatory agents of isolated trachea, main bronchi (using classic organ bath) and isolated bronchioles (using small myograph) from wistar and fisher 344 rats. the largest contraction was elicited on all preparations by carbachol. histamine elicited a (small) contraction only ... | 1998 | 9716302 |
| enhanced isoflurane suppression of excitatory synaptic transmission in the aged rat hippocampus. | 1. the effects of the volatile anaesthetic, isoflurane, were investigated on evoked dendritic field excitatory postsynaptic potentials (f.e.p.s.p.) and antidromic and orthodromic population spikes recorded extracellularly in the ca1 cell layer region in the in vitro hippocampal slice taken from young mature (2-3 months) and old (24-27 months) fisher 344 rats. 2. isoflurane depressed the f.e.p.s.ps and the orthodromically-evoked population spikes in both old and young hippocampi. however, the mag ... | 1998 | 9720776 |
| reactivities of cys707 (sh1) in intermediate states of myosin subfragment-1 atpase. | to detect structural changes around the reactive cys707 (sh1) in the myosin heavy chain during the atpase reaction, the reactivity of sh1 in rabbit skeletal myosin subfragment-1 (s-1) was measured using a fluorescent reagent, 5-(iodoacetamidoethyl)aminonaphthalene-1-sulfonic acid, in the presence of various atp analogs: adenosine 5'-(3-thiotriphosphate) (atpgammas), adp-vanadate (adp-vi), adp-befx, and adp-alf4. the sh1 reactivities in the s-1 complexes with atpgammas and adp-befx, analogs of th ... | 1998 | 9722673 |
| medial smooth muscle cell loss in arterial allografts occurs by cytolytic cell induced apoptosis. | experimental arterial allografts, used as models of chronic rejection, undergo marked loss of smooth muscle cells (smc) from their media prior to the development of occlusive, intimal proliferative lesions. medial smc loss has been described in human heart transplants, and may be related to the development of occlusive coronary lesions which are the hallmark of chronic rejection. this smc loss does not exhibit the characteristics of necrotic cell death. we sought to determine whether medial smc ... | 1998 | 9726621 |
| increased activity improves recovery of partially denervated fast rat muscles. | partial denervation of the neonatal rat extensor digitorum longus muscles by removing the l4 spinal nerve and thus 80% of its innervation [a.l. connold, t.j. fisher, s. maudarbocus, g. vrbová, response of developing fast muscles to partial denervation, neuroscience 46 (1992) 981-988; f. tyc, g. vrbová, the effect of partial denervation of developing rat fast muscles on their motor unit properties, j. physiol. 482 (1995) 651-660] results in its permanent weakness. the possibility that the weaknes ... | 1998 | 9729347 |
| nutritional status modulates rat liver cytochrome p450 arachidonic acid metabolism. | alterations in nutritional status affect hepatic cytochrome p450 levels. since cytochromes p450 participate in the metabolism of arachidonic acid, we hypothesized that changes in liver p450 arachidonic acid metabolism occur during fasting and refeeding. male fisher 344 rats were either fed, fasted 48 hr (f48), fasted 48 hr and then refed 6 hr (f48/r6), or fasted 48 hr and then refed 24 hr (f48/r24). f48 rats had reduced body weight, increased plasma beta-hydroxybutyrate, and reduced plasma insul ... | 1998 | 9730909 |
| caloric restriction prevents age-related decline in skeletal muscle dihydropyridine receptor and ryanodine receptor expression. | the dihydropyridine receptor (dhpr), a voltage-gated l-type ca2+ channel, and the ca2+ release channel/ryanodine receptor isoform-1 (ryr1) are key molecules involved in skeletal muscle excitation-contraction coupling. we have reported age-related decreases in the level of dhpr expression in fast- and slow-twitch muscles from fisher 344 cross brown norway (f344bnx) rats (renganathan, messi and delbono, j. membr. biol. 157 (1997) 247-253). based on these studies we postulate that excitation-contra ... | 1998 | 9742952 |
| antioxidant effects of n-acetylcysteine and succimer in red blood cells from lead-exposed rats. | this study examined whether lead-induced alterations in selected parameters that are indicative of oxidative stress accompany the toxic effects of lead in red blood cells (rbcs) in vivo. it also explored the possibility that treatment with n-acetylcysteine (nac) or succimer (meso-2,3-dimercaptosuccinic acid) was capable of reversing parameters indicative of lead-induced oxidative stress. fisher 344 rats were given 2000 ppm lead acetate in their drinking water for 5 weeks. the lead was then remov ... | 1998 | 9750041 |
| rapid hybrid speciation in wild sunflowers. | hybrid or "recombinational" speciation refers to the origin of a new homoploid species via hybridization between chromosomally or genetically divergent parental species. theory predicts that this mode of speciation is punctuated, but there has been little empirical evidence to support this claim. here, we test the hypothesis of rapid hybrid speciation by estimating the sizes of parental species chromosomal blocks in helianthus anomalus, a wild sunflower species derived via hybridization between ... | 1998 | 9751738 |
| pre- and postsynaptic blockade of neuromuscular transmission by miller-fisher syndrome igg at mouse motor nerve terminals. | miller-fisher syndrome, a variant of an acute inflammatory neuropathy is often associated with serum antibodies to the ganglioside gq1b, but the pathogenic role of these antibodies and other serum factors is unclear. we here investigated the effect of highly purified immunoglobulin g (igg) from patients with typical miller-fisher syndrome, recording quantal endplate currents by means of a perfused macro-patch-clamp electrode on hemidiaphragms of adult mice. the gq1b-positive and the gq1b-negativ ... | 1998 | 9753137 |
| immunotargeting of catalase to ace or icam-1 protects perfused rat lungs against oxidative stress. | the pulmonary endothelium is susceptible to oxidative insults. catalase conjugated with monoclonal antibodies (mabs) against endothelial surface antigens, angiotensin-converting enzyme (mab 9b9) or intercellular adhesion molecule-1 (mab 1a29), accumulates in the lungs after systemic injection in rats (v. muzykantov, e. atochina, h. ischiropoulos, s. danilov, and a. fisher. proc. natl. acad. sci. usa 93: 5213-5218, 1996). the present study characterizes the augmentation of antioxidant defense by ... | 1998 | 9755114 |
| sex and the evolution of intrahost competition in rna virus phi6. | sex allows beneficial mutations that occur in separate lineages to be fixed in the same genome. for this reason, the fisher-muller model predicts that adaptation to the environment is more rapid in a large sexual population than in an equally large asexual population. sexual reproduction occurs in populations of the rna virus phi6 when multiple bacteriophages coinfect the same host cell. here, we tested the model's predictions by determining whether sex favors more rapid adaptation of phi6 to a ... | 1998 | 9755186 |
| continuity between wound macrophage and fibroblast phenotype: analysis of wound fibroblast phagocytosis. | analysis of phagocytic activity in wound fibroblasts was chosen as a means to assess the possible continuity between macrophage and fibroblast phenotypes. fibroblast phagocytosis of uncoated, igg-coated, or collagen-coated fluorescent beads was analyzed by flow cytometry in vivo and in vitro. phagocytosis of fluorescent beads by procollagen i-positive cells (fibroblasts) was evaluated in vivo by injecting beads into subcutaneously implanted sponge wounds in anesthetized fisher rats. phagocytic a ... | 1998 | 9756532 |
| isolated lung liposome-mediated gene transfer produces organ-specific transgenic expression. | gene therapy is a promising strategy for the treatment of inoperable pulmonary tumors and rejection after lung transplantation. however, unlike ex vivo administration, intravenous in vivo transfection lacks organ specificity and has a limited duration of expression. the objectives of this study were to limit transfection to a single lung and to increase the duration of gene expression in vivo. | 1998 | 9768949 |
| detection of 400-year-old yersinia pestis dna in human dental pulp: an approach to the diagnosis of ancient septicemia. | ancient septicemic plague epidemics were reported to have killed millions of people for 2 millenniums. however, confident diagnosis of ancient septicemia solely on the basis of historical clinical observations is not possible. the lack of suitable infected material has prevented direct demonstration of ancient septicemia; thus, the history of most infections such as plague remains hypothetical. the durability of dental pulp, together with its natural sterility, makes it a suitable material on wh ... | 1998 | 9770538 |
| histological characterization and pharmacological control of chronic rejection in xenogeneic and allogeneic heart transplantation. | chronic allograft rejection remains a major barrier to successful long-term allograft transplantation in humans. chronic allograft rejection is characterized by the appearance of arterial lesions with concentric intimal thickening. this study investigates the development and control of chronic rejection in hamster cardiac xenografts transplanted into lewis rats. | 1998 | 9771831 |
| chronic allograft nephropathy in the rat is improved by angiotensin ii receptor blockade but not by calcium channel antagonism. | functional and structural changes of chronic renal allograft failure share similarities with other chronic nephropathies with low nephron number. in models of reduced nephron number, angiotensin-converting enzyme inhibitors or angiotensin ii receptor blockers prevented proteinuria and retarded renal lesions. this study investigates whether blockade of angiotensin ii activity prevented chronic allograft injury in the fisher 344 --> lewis rat kidney transplant model, and compares its effect with t ... | 1998 | 9773797 |
| [recurrent miller fisher syndrome and anti-gq1b antibodies]. | introduction: miller-fisher syndrome is defined by the triad: ophthalmoplegia, ataxia and areflexia. this rare entity is generally regarded as a variant of the guillain-barré syndrome, although neurophysiological patterns differ. in the acute phase of the disease, sera of affected patients contain high titers of antiganglioside anti-gq1b, which is a specific marker. recurrences are exceptional. exegese: we report the case of a man with three recurrences of miller-fisher syndrome within 16 years. ... | 1998 | 9775140 |