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molecular diagnosis of ehrlichia canis in dogs and ticks rhipicephalus sanguineus (acari: ixodidae) in yucatan, mexico.ehrlichia canis is the etiological agent behind canine monocytic ehrlichiosis, and the tick rhipicephalus sanguineus (latreille) is its main vector. blood smear and nested polymerase chain reaction (pcr) techniques were used to identify e. canis infection in dogs and r. sanguineus, and explore factors possibly associated with infection in dogs in yucatan, mexico. blood samples were taken and ticks r. sanguineus collected from 50 dogs (10 house dogs and 40 in an animal control center). data were ...201526336286
seroprevalence of tick-borne pathogens and tick infestation in dogs from tapirapé and karajá indigenous communities, brazil.with the aim of studying some tick-borne diseases, a total of 327 dogs (114 from tapirapé and 213 from karajá indigenous ethnicity, mato grosso, mt, brazil) were sampled. serum samples were submitted to the indirect fluorescence antibody test (ifat) to detect antibodies against babesia vogeli, ehrlichia canis, and rickettsia spp. possible associations of risk factors and the occurrence of seroreativity to tick-borne agents and tick infestations were analyzed through chi-squared tests. among 327 ...201526186512
the efficacy of a generic doxycycline tablet in the treatment of canine monocytic ehrlichiosis.the objective of the present study was to evaluate the therapeutic efficacy of a generic doxycycline tablet (doxyvet) against ehrlichia canis infection in dogs. canine monocytic ehrlichiosis is caused by the bacterium e. canis and transmitted by the brown kennel tick (rhipicephalus sanguineus). six disease-free and tick-free dogs were infested with e. canis infected ticks. once diagnosed (with polymerase chain reaction [pcr] analysis and platelet counts) as positive for infection, doxycycline ta ...201526018824
survey of ehrlichia canis, babesia spp. and hepatozoon spp. in dogs from a semiarid region of brazil.this study assessed the occurrence of ehrlichia spp., babesia spp. and hepatozoon spp. infections in 100 tick-harboring dogs from a semiarid region of the state of paraíba, northeastern brazil. blood samples and ticks were collected from the animals, and a questionnaire was submitted to dog owners to obtain general data. blood samples were used to perform hemogram, direct blood smear and immunological and molecular hemoparasite detection. the 1,151 ticks collected were identified as rhipicephalu ...201525909253
a serological and molecular survey of babesia vogeli, ehrlichia canis and rickettsia spp. among dogs in the state of maranhão, northeastern brazil.this study evaluated exposure and infection by tick-borne agents (babesia vogeli, ehrlichia canis and rickettsia spp.) in 172 dogs in rural areas and 150 dogs in urban areas of the municipality of chapadinha, state of maranhão, northeastern brazil, using molecular and serological methods. overall, 16.1% of the sampled dogs (52/322) were seroreactive to b. vogeli, with endpoint titers ranging from 40 to 640. for e. canis, 14.6% of the dogs (47/322) were seroreactive, with endpoint titers from 80 ...201525909250
further thoughts on the taxonomy and vector role of rhipicephalus sanguineus group ticks.rhipicephalus sanguineus is a tick species described in 1806 by latreille, based on specimens probably collected in france. however, this is a taxon with uncertain morphological definition and recent studies have gathered irrefutable evidence supporting the existence of a cryptic species-complex under the name 'r. sanguineus', whose number of sibling species around the world has yet to be ascertained. this fact is of great medical and veterinary concern, also considering that ticks currently ide ...201525579394
transmission of ehrlichia canis by rhipicephalus sanguineus ticks feeding on dogs and on artificial membranes.a south african strain of ehrlichia canis was isolated and used to infect a laboratory-bred beagle dog. rhipicephalus sanguineus nymphs, which fed on this dog, moulted to adult ticks which carried infection rates of e. canis between 12% and 19% and were used in a series of in vivo and in vitro experiments. five groups of 6 dogs were challenged with the infected r. sanguineus ticks, which were removed 24h, 12h, 6h or 3h after the ticks had been released onto the dogs. the animals were monitored f ...201323962826
imidacloprid 10 % / flumethrin 4.5 % collars (seresto®, bayer) successfully prevent long-term transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs.the objective of this study was to determine the empirical efficacy of imidacloprid 10 %/flumethrin 4.5 % (seresto®) collars in preventing long-term transmission of ehrlichia canis by infected rhipicephalus sanguineus ticks to dogs. the study was a parallel group design, single centre, randomised, non-blinded, controlled, long-term efficacy study. the treatment group of 8 dogs was fitted with seresto® collars, the untreated control group of 8 dogs received no collars. ehrlichia canis-infected ti ...201323774841
prevention of transmission of ehrlichia canis by rhipicephalus sanguineus ticks to dogs treated with a combination of fipronil, amitraz and (s)-methoprene (certifect®).the ability of certifect(®) (a combination of fipronil, amitraz and (s)-methoprene) to prevent transmission of ehrlichia canis was studied in two groups of eight dogs. one group was treated with certifect while the other group remained untreated. all dogs were exposed to e. canis-infected rhipicephalus sanguineus ticks on days 7, 14, 21 and again on day 28 post-treatment by releasing ticks into the kennels of the dogs to simulate the natural way of infestation. animals were examined in situ for ...201323298566
serological survey on ehrlichia sp. among dogs in the central region of rio grande do sul.a serological survey on ehrlichia canis was conducted among dogs in the central area of the state of rio grande do sul, where the tick rhipicephalus sanguineus is a common parasite of dogs. out of a total of 316 dogs attended at the veterinary teaching hospital in the municipality of santa maria, only 14 (4.43%) reacted positively to e. canis antigens in the indirect immunofluorescence assay, with the following endpoint titers: 80 (three dogs), 160 (five), 320 (four), 640 (one) and 1280 (one). l ...201323184320
ehrlichia canis in rhipicephalus sanguineus ticks in the ivory coast.canine monocytic ehrlichiosis caused by ehrlichia canis is distributed globally, but its prevalence in africa is poorly known. in the study reported herein, 27% of rhipicephalus sanguineus ticks collected from watchdogs in abidjan, ivory coast, were positive for e. canis using quantitative real-time pcr. a new molecular strategy is proposed that can be used not only for epidemiological study, but also for the diagnosis of canine monocytic ehrlichiosis. our findings show for the first time the pr ...201223141106
mitochondrial dna analysis of rhipicephalus sanguineus sensu lato (acari: ixodidae) in the southern cone of south america.a genetic analysis of partial sequences of the mitochondrial 16s and 12s rdna genes of rhipicephalus sanguineus sensu lato collected in the southern cone of south america was carried out. also, sequences of ticks belonging to this taxon from europe, africa and other south american localities were included. tcs networks constructed with 16s rdna sequences showed two clusters of haplotypes, namely, southern lineage (st) and northern lineage (nt). haplotypes representing the specimens coming from l ...201222818199
detection of francisella-like endosymbiont in hyalomma rufipes from ethiopia.the expanding family of francisellaceae includes the genus francisella, where several pathogen bacteria, e.g. the zoonotic f. tularensis, and different francisella-like agents belong to. francisella-like endosymbionts (fles) are widespread in hard and soft ticks and their pathogenicity is unknown. the examination of 296 ticks collected in ethiopia was performed for the detection of f. tularensis and fles using polymerase chain reaction (pcr) assays based on the amplification of 16s rrna, sdha an ...201425108781
retrospective molecular study on canine hepatozoonosis in slovakia - does infection risk for dogs really exist?this study provides the very first evidence of hepatozoon canis infection in dogs from slovakia, a rhipicephalus sanguineus sensu lato free area. in total, 297 red foxes (vulpes vulpes) and 293 dogs, from three regions of slovakia, were screened for the presence of h. canis using a conventional 18s rrna polymerase chain reaction (pcr). genomic dna of this blood parasite was detected in 51 (17.1%) foxes from all sampling regions in slovakia, whereas the overall prevalence in examined dogs was sig ...201728366618
feline and canine leishmaniosis and other vector-borne diseases in the aeolian islands: pathogen and vector circulation in a confined environment.vector-borne diseases (vbds) are prevalently investigated in dogs. studies on feline vbds are scant, though feline leishmaniosis (fel) is increasingly recognised as a disease of cats in endemic areas. comprehensive investigations on the distribution of vbds in populations of cats and dogs living in relatively small geographical areas, such as islands, are currently lacking. in this study the prevalence of leishmania infantum and other vbd pathogens was assessed in cohorts of cats and dogs living ...201728288759
rhipicephalus turanicus, a new vector of hepatozoon canis.the distribution of hepatozoon canis mainly encompasses areas where its main tick vector, rhipicephalus sanguineus sensu lato, is present. however, the detection of this pathogen in dogs, foxes and golden jackals well outside the areas inhabited by this tick species reinforced the hypothesis that additional ixodids are involved in the life cycle and transmission of this protozoon. the present study provides, for the first time, data supporting the sporogonic development of h. canis in specimens ...201627998321
detection and molecular identification of hepatozoon canis and babesia vogeli from domestic dogs in palestine.dogs serve as hosts for a great number of parasites, which may affect their health and wellbeing. this study aimed to observe tick borne pathogens in dogs from palestine including hepatozoon canis and babesia species. the prevalence of both h. canis and babesia species infections in apparently healthy dogs, from ten districts of the west bank was surveyed. dna was extracted from blood samples obtained from dogs (n = 362) and ticks (n = 213) collected from dogs (n = 77). a primer set that amplifi ...201727938422
autochthonous hepatozoon infection in hunting dogs and foxes from the czech republic.blood samples from 21 red foxes (vulpes vulpes) and 8 hunting dogs from the same locality in the czech republic were examined for presence of hepatozoon canis/hepatozoon sp. the dogs were selected based on their close contact with foxes during fox bolting and because they had not traveled into known endemic areas. using diagnostic pcr amplifying partial 18s rdna fragment, hepatozoon dna was detected in 20 red foxes (95 %) and 4 dogs (50 %). from 8 positive foxes and 2 positive dogs, we obtained ...201627431805
molecular detection and phylogenetic analysis of hepatozoon spp. in questing ixodes ricinus ticks and rodents from slovakia and czech republic.by amplification and sequencing of 18s rrna gene fragments, hepatozoon spp. dna was detected in 0.08 % (4/5057) and 0.04 % (1/2473) of questing ixodes ricinus ticks from slovakia and czech republic, respectively. hepatozoon spp. dna was also detected in spleen and/or lungs of 4.45 % (27/606) of rodents from slovakia. prevalence of infection was significantly higher in myodes glareolus (11.45 %) than in apodemus spp. (0.28 %) (p < 0.001). sequencing of 18s rrna hepatozoon spp. gene amplicons from ...201627245074
occurrence of hepatozoon canis and cercopithifilaria bainae in an off-host population of rhipicephalus sanguineus sensu lato ticks.hepatozoon canis (eucoccidiorida, hepatozoidae) and the filarioid cercopithifilaria bainae (spirurida, onchocercidae) are tick-transmitted infectious agents of dogs, highly prevalent in the mediterranean basin in association with rhipicephalus sanguineus sensu lato. ticks were collected from the environment every 25±2 days in a confined location in southern italy where a community of dogs lives, from august 2012 to july 2013. in order to study the occurrence of h. canis and c. bainae, 1091 tick ...201424594107
failure of imidocarb dipropionate and toltrazuril/emodepside plus clindamycin in treating hepatozoon canis infection.hepatozoonosis caused by hepatozoon canis (eucoccidiorida, hepatozoidae) is among the most widespread vector-borne infections of dogs, primarily transmitted by rhipicephalus sanguineus sensu lato ticks. based on the absence of a consensus on the treatment regimes for canine hepatozoonosis, the present study aimed to evaluate the efficacy of imidocarb dipropionate (5-6 mg/kg subcutaneously once a week for 6 weeks), and of toltrazuril/emodepside (procox(®), 15 mg/kg once a day for 6 days) in assoc ...201424439769
investigation of tick vectors of hepatozoon canis in brazil.hepatozoon canis is a common apicomplexan parasite of dogs. in brazil, in addition to rhipicephalus sanguineus, amblyomma ovale, amblyomma cajennense, and rhipicephalus (boophilus) microplus have been suggested to act as vectors. the present study aimed to evaluate, under controlled conditions, the acquisition of h. canis by a. ovale, r. sanguineus, and a. cajennense after feeding on naturally infected dogs. cytological and histophatological examinations were performed to recover oocysts and oth ...201324209494
experimental evidence against transmission of hepatozoon canis by ixodes ricinus.hepatozoon canis is among the most widespread tick-borne protozoa infecting domestic and wild carnivores. its distribution is related to the occurrence of its major vector, the brown dog tick rhipicephalus sanguineus. however, the role of ixodes ricinus as a vector of h. canis has been hypothesized. in the present study, the development of h. canis was investigated in i. ricinus and r. sanguineus nymphs collected from a naturally infested dog. all i. ricinus ticks examined (n=133) were negative ...201323727151
transstadial transmission of hepatozoon canis from larvae to nymphs of rhipicephalus sanguineus.hepatozoon canis is an apicomplexan parasite of dogs, which is known to become infected by ingesting rhipicephalus sanguineus adult ticks. to investigate the possibility of h. canis transovarial and transstadial transmission from larvae to nymphs, engorged adult female ticks were collected from a private animal shelter in southern italy, where h. canis infection is highly prevalent. female ticks (n=35) and egg batches were tested by pcr for h. canis. all eggs examined were pcr-negative whereas 8 ...201323537949
molecular investigations of hepatozoon species in dogs and developmental stages of rhipicephalus sanguineus.the occurrence and distribution of hepatozoon species in stray dogs, and the developmental stages of rhipicephalus sanguineus detached from the same dogs in diyarbakır province, turkey is reported. a total of 328 ticks, including 133 adults (55 males and 75 females consist of 63 partially engorged and 15 fully engorged) and 195 nymphs (91 partially engorged and 104 fully engorged) were detached from the dogs. fully engorged nymphs and females were incubated at 27 °c and relative humidity of 85 % ...201323535887
high prevalence of hepatozoon-infection among shepherd dogs in a region considered to be free of rhipicephalus sanguineus.blood samples and ticks were collected from 100 shepherd dogs, 12 hunting dogs and 14 stray dogs in southern hungary, in order to screen them for the presence of hepatozoon spp. by pcr. out of 126 blood samples, 33 were positive (26%). significantly more shepherd dogs (31%) were infected, than hunting (8%) and stray dogs (7%). three genotypes of hepatozoon canis were identified by sequencing, differing from each other in up to six nucleotides in the amplified portion of their 18s rrna gene. in d ...201323499483
a golden jackal (canis aureus) from austria bearing hepatozoon canis--import due to immigration into a non-endemic area?the protozoan hepatozoon canis, which is transmitted via ingestion of infected ticks by canine hosts, is not endemic to mid-latitude regions in europe. its distribution is supposed to be linked to the occurrence of its primary tick vector rhipicephalus sanguineus. a young male golden jackal (canis aureus) found as road kill close to vienna, austria, was infected by this pathogen. cloning and sequencing of the pcr product revealed 6 different haplotypes of h. canis. based on the sequences, no cle ...201323306030
apparent tick paralysis by rhipicephalus sanguineus (acari: ixodidae) in dogs.certain tick species including ixodes holocyclus can inoculate neurotoxins that induce a rapid, ascending flaccid paralysis in animals. rhipicephalus sanguineus, the most widespread tick of dogs, is recognized as a vector of several pathogens causing diseases in dogs and humans. a single report suggests its role as cause of paralysis in dogs. this study presents the clinical history of 14 young dogs heavily infested by r. sanguineus (intensity of infestation, 63-328) in an endemic area of southe ...201222546547
natural transovarial and transstadial transmission of leishmania infantum by naïve rhipicephalus sanguineus ticks blood feeding on an endemically infected dog in shiraz, south of iran.the visceral leishmaniasis parasite, leishmania infantum, is naturally transmitted through the bites of phlebotomine sand flies. alternative routes of transmission are questioned. the main aim is to verify the passage of l. infantum kdna in ticks, rhipicephalus sanguineus, blood feeding on a parasitemic dog in shiraz, south of iran.201627496515
hemocytes of rhipicephalus sanguineus (acari: ixodidae): characterization, population abundance, and ultrastructural changes following challenge with leishmania infantum.few studies have examined the cellular immune response of ticks, and further research on the characterization of the hemocytes of ticks is required, particularly on those of rhipicephalus sanguineus (latreille) because of the medical and veterinary importance of this tick. the aims of this study were to characterize the morphology and the ultrastructure of the different types of hemocytes of adult r. sanguineus and to determine the population abundance and the ultrastructural changes in the hemo ...201526336264
determination the role of rhipicephalus sanguineus for transmission of leishmania major to reservoir animals.this study aimed to explore the role of rhipicephalus sanguineus in the transmission of leishmania major, the etiological agent of zoonotic cutaneous leishmaniasis.201628091384
heat-stressed metarhizium anisopliae: viability (in vitro) and virulence (in vivo) assessments against the tick rhipicephalus sanguineus.the current study investigated the thermotolerance of metarhizium anisopliae s.l. conidia from the commercial products metarril® sp organic and metarril® wp. the efficacy of these m. anisopliae formulations against the tick rhipicephalus sanguineus s.l. was studied in laboratory under optimum or heat-stress conditions. the products were prepared in water [tween® 80, 0.01 % (v/v)] or pure mineral oil. conidia from metarril® sp organic suspended in water presented markedly delayed germination afte ...201727704216
effect of heat stress and oil formulation on conidial germination of metarhizium anisopliae s.s. on tick cuticle and artificial medium.the effect of heat stress (45°c) versus non-heat stress (27°c) on germination of metarhizium anisopliae sensu stricto (s.s.) isolate ip 119 was examined with conidia formulated (suspended) in pure mineral oil or in water (tween 80, 0.01%), and then applied onto the cuticle of rhipicephalus sanguineus sensu lato (s.l.) engorged females or onto culture medium (pday). in addition, bioassays were performed to investigate the effect of conidia heated while formulated in oil, then applied to blood-eng ...201627317831
efficacy of water- and oil-in-water-formulated metarhizium anisopliae in rhipicephalus sanguineus eggs and eclosing larvae.conidia of the entomopathogenic fungus metarhizium anisopliae (ascomycota: clavicipitaceae) were assessed against rhipicephalus sanguineus (arachnida: ixodidae) eggs under laboratory conditions. clusters of 25 eggs were applied either directly with the fungal conidial formulations or set on previously fungus-treated filter paper. treatments consisted of conidia formulated in water or an oil-in-water emulsion at final concentrations of 3.3 × 10(3), 10(4), 3.3 × 10(4), 10(5), or 3.3 × 10(5) conidi ...201626364059
resistant ticks inhibit metarhizium infection prior to haemocoel invasion by reducing fungal viability on the cuticle surface.we studied disease progression of, and host responses to, four species in the metarhizium anisopliae complex expressing green fluorescent protein (gfp). we compared development and determined their relative levels of virulence against two susceptible arthropods, the cattle tick rhipicephalus annulatus and the lepidopteran galleria mellonella, and two resistant ticks, hyalomma excavatum and rhipicephalus sanguineus. metarhizium brunneum ma7 caused the greatest mortality of r. annulatus, metarhizi ...201222507442
occurrence of mycoplasma haemocanis in dogs infested by ticks in campo grande, mato grosso do sul, brazil.hemotropic mycoplasmas in dogs, such as mycoplasma haemocanis, have been described worldwide. recently, these pathogens have been reported to be causative agent of zoonosis. it is known that its transmission may occur through the action of blood-sucking arthropods (e.g. ticks or fleas), through blood transfusion, contaminated fomites and/or transplacentally. in brazil, m. haemocanis is present in practically all regions and the tick rhipicephalus sanguineus sensu lato is suspected the main vecto ...201627580391
characterisation of ecto- and endoparasites in domestic cats from tirana, albania.during 2008 to 2011, faecal samples, ear swabs, and ectoparasites obtained by full body search and total body comb were collected from 252 cats originating from the greater tirana area. faecal samples were examined using the mcmaster and baermann techniques, and a subset of 58 samples was tested for giardia-specific antigen using a coproantigen enzyme-linked immunosorbent assay (elisa). the ear swabs were examined for the presence of parasitic mites. overall, almost 93% of the cats were identifi ...201424994454
detection of rickettsia hoogstraalii, rickettsia helvetica, rickettsia massiliae, rickettsia slovaca and rickettsia aeschlimannii in ticks from sardinia, italy.tick-borne diseases represent a large proportion of infectious diseases that have become a world health concern. the presence of rickettsia spp. was evaluated by standard pcr and sequencing in 123 ticks collected from several mammals and vegetation in sardinia, italy. this study provides the first evidence of the presence of rickettsia hoogstralii in haemaphysalis punctata and haemaphysalis sulcata ticks from mouflon and rickettsia helvetica in ixodes festai ticks from hedgehog. in addition, ric ...201728110915
molecular detection and characterization of spotted fever group rickettsiae in ticks from central italy.the aim of this study was to investigate the presence of rickettsial pathogens in ticks from central italy. a total of 113 ticks hailed from latium and tuscany regions were identified and tested by pcr to detect glta, ompa, ompb genes of rickettsia. positive amplicons were sequenced and identified at species level. ticks were analyzed individually or in pools. the percentage of positivity for sfg rickettsiae was 12.4%, expressed as minimum infection rate (mir) assuming that one tick was positive ...201627365155
identification of tick species and disseminate pathogen using hemolymph by maldi-tof ms.matrix assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) is increasingly emerging tool for identification of arthropods including tick vectors using whole or body part of specimens. the challenges of the present study were to assess maldi-tof ms profiling for the both identification of tick species and rickettsia spp. in infected ticks using hemolymph as protein mixture.201526051210
spotted fever group rickettsiae in ticks and fleas from the democratic republic of the congo.little is known about the prevalence of spotted fever group (sfg) rickettsiae in ticks and fleas in the democratic republic of the congo. in 2008, 12 amblyomma compressum ticks were collected from 3 pangolins (manis gigantea). two haemaphysalis punctaleachi ticks were collected from 2 african civets (civettictis civetta congica), and one was collected from an antelope (onotragus leche). a total of 111 rhipicephalus sanguineus ticks, 23 ctenocephalides canis fleas, 39 c. felis fleas, and 5 tricho ...201223137572
detection of rickettsiae in fleas and ticks from areas of costa rica with history of spotted fever group rickettsioses.outbreaks of spotted fevers have been reported in costa rica since the 1950s, although vectors responsible for transmission to humans have not been directly identified. in this study, species of rickettsia were detected in ectoparasites from costa rica, mostly from five study sites where cases of spotted fevers have been reported. ticks and fleas were collected using drag cloths or directly from domestic and wild animals and pooled according to species, host, and location. pools were analyzed in ...201627592065
rickettsia (rickettsiales: rickettsiaceae) vector biodiversity in high altitude atlantic forest fragments within a semiarid climate: a new endemic area of spotted-fever in brazil.rickettsioses are re-emerging vector-borne zoonoses with a global distribution. recently, rickettsia sp. strain atlantic rainforest has been associated with new human spotted-fever (sf) cases in brazil, featuring particular clinical signs: eschar formation and lymphadenopathy. these cases have been associated with the tick species, amblyomma ovale from 2010 until 2015, the brazilian health department confirmed 11 human sf cases in the maciço de baturité region, ceará, brazil. the present study r ...201627480099
genetic characterization of spotted fever group rickettsiae in questing ixodid ticks collected in israel and environmental risk factors for their infection.this study aimed to genetically characterize spotted fever group rickettsiae (sfgr) in questing ixodid ticks from israel and to identify risk factors associated with sfgr-positive ticks using molecular techniques and geographic information systems (gis) analysis. 1039 ticks from the genus rhipicephalus were collected during 2014. 109/1039 (10·49%) carried sfgr-dna of either rickettsia massiliae (95), 'candidatus rickettsia barbariae' (8) or rickettsia conorii (6). higher prevalence of sfgr was f ...201728330517
mediterranean spotted fever: a rare non-endemic disease in the usa.we report a case of a 43-year-old israeli male who presented with an intermittent fever associated with a gradual appearance of diffusely scattered erythematous non-pruritic maculopapular lesions, generalized body malaise, muscle aches, and distal extremity weakness. he works in the israeli military and has been exposed to dogs that are used to search for people in tunnels and claimed that he had removed ticks from the dogs. in the hospital, he presented with fever, a diffuse maculopapular rash, ...201728191378
the role of rhipicephalus sanguineus sensu lato saliva in the dissemination of rickettsia conorii in c3h/hej mice.animal models have been developed for the study of rickettsial pathogenesis. however, to understand what occurs during the natural route of rickettsial transmission via the tick bite, the role of tick saliva should be considered in these models. to address this, we analysed the role of tick saliva in the transmission of rickettsia conorii (rickettsiales: rickettsiaceae) in a murine host by intradermally (i.d.) inoculating two groups of susceptible c3h/hej mice with this rickettsia, and infesting ...201526011701
mediterranean spotted fever and hearing impairment: a rare complication.mediterranean spotted fever (msf) is caused by rickettsia conorii and transmitted by the brown dog tick rhipicephalus sanguineus. it is prevalent in southern europe, africa and central asia. the disease usually has a benign course and is characterized by fever, myalgia and a characteristic papular rash with an inoculation eschar ('tache noir') at the site of the tick bite. severe forms of disease can have cardiac, neurologic or renal involvement. nervous system complications are unusual and may ...201525892247
rickettsia conorii israelensis in rhipicephalus sanguineus ticks, sardinia, italy.the presence of tick-borne rickettsia spp. was examined by pcr using dna samples extracted from 254 ticks collected from mammals originating from northern and eastern sardinia, italy. the spotted fever group rickettsial agent rickettsia conorii israelensis was detected in 3 rhipicephalus sanguineus ticks from a dog for the first time in this geographical area. in addition, ri. massiliae, ri. slovaca, and ri. aeschlimannii were detected in rh. turanicus, rh. sanguineus, dermacentor marginatus, an ...201424852264
levels of certain endothelial biomarkers during the acute phase and convalescence in patients with different severity of mediterranean spotted fever.mediterranean spotted fever (msf) is a tick-borne disease caused by rickettsia conorii subspp. conorii. it is transmitted by the bite of the tick rhipicephalus sanguineus. modified by the rickettsial invasion, the micro-vascular endothelium acquires an activated inflammatory phenotype and initiates secretion of cytokines and expression of cell adhesion molecules (cams) and chemoattractans.201524712278
effects of homologous and heterologous immunization on the reservoir competence of domestic dogs for rickettsia conorii (israelensis).a number of spotted fever group (sfg) rickettsiae cause serious infections in humans. several antigenically related rickettsial agents may coexist within the same geographical area, and humans or vertebrate hosts may be sequentially exposed to multiple sfg agents. we assessed whether exposure of a vertebrate reservoir to one sfg rickettsia will affect the host's immune response to a related pathogen and the efficiency of transmission to uninfected ticks. two pairs of dogs were each infected with ...201424201056
urban family cluster of spotted fever rickettsiosis linked to rhipicephalus sanguineus infected with rickettsia conorii subsp. caspia and rickettsia massiliae.here, we report an epidemiological and entomological investigation of a cluster of cases of spotted fever group (sfg) rickettsiosis occurring in southern france. a family of 3 (husband, wife, and their son) presented with symptoms compatible with sfg rickettsiosis. for 2 patients, serum samples presented increased levels of igm and igg for sfg rickettsia. the patients' home was investigated, and rhipicephalus sanguineus ticks were collected from the floor from behind the furniture. of 22 ticks c ...201223140893
some epidemiological features of the mediterranean spotted fever re-emerging in bulgaria.the mediterranean spotted fever (msf), caused by rickettsia conorii conorii strain malish, is transmitted by the brown dog tick rhipicephalus sanguineus. in bulgaria, cases of msf occurred in two epidemic waves, the first in 1948-1970, (after there were no report of new cases more than for 20 years) and the second started in 1993 and is still going on. the aim of the study was to investigate the epidemiological characteristics of the re-emerging msf in plovdiv city and its suburbs, which is the ...201222908829
rickettsia parkeri: a rickettsial pathogen transmitted by ticks in endemic areas for spotted fever rickettsiosis in southern uruguay.at first rickettsia conorii was implicated as the causative agent of spotted fever in uruguay diagnosed by serological assays. later rickettsia parkeri was detected in human-biting amblyomma triste ticks using molecular tests. the natural vector of r. conorii, rhipicephalus sanguineus, has not been studied for the presence of rickettsial organisms in uruguay. to address this question, 180 r. sanguineus from dogs and 245 a. triste from vegetation (flagging) collected in three endemic localities w ...201222634883
[mediterranean spotted fever in paediatric and adult patients: two clinical aspects of the same disease].mediterranean spotted fever is an acute febrile disease caused by rickettsia conorii and transmitted to humans by the brown dog tick rhipicephalus sanguineus. nearly 400 cases are reported every year in sicily, mainly from june to september. the aim of this study is to compare the clinical and laboratory features of two different groups of patients , one of adults and one of children. the analysis included all adult patients with msf diagnosed at the institute of infectious diseases, paolo giacc ...201122212164
"candidatus rickettsia asemboensis" in rhipicephalus sanguineus ticks, brazil."candidatus rickettsia asemboensis" is an obligate intracellular bacterium of the rickettsiales order, genetically related to species belonging to the rickettsia felis group, agents of flea-borne spotted fever. here we report for the first time the detection of "ca. r. asemboensis", a flea-associated organism, in rhipicephalus sanguineus ticks. it is the first occurrence of this emerging bacterium in brazil, which increases the geographical distribution of this r. felis-like agent.201727986544
fleas and ticks in carnivores from a domestic-wildlife interface: implications for public health and wildlife.fleas and ticks are parasites of wild and domestic mammals, and can be vectors of several pathogens. in rural areas, domestic carnivores such as the domestic dog (canis lupus familiaris l.), may act as a "bridge" between natural areas and human settlements where ectoparasites can be used as a metric of such link. the aim of this study was to identify fleas, ticks, and rickettsia spp., collected from domestic and wild carnivores in a natural reserve and surrounding human settlements in central ch ...201627539151
exposure of dogs to spotted fever group rickettsiae in urban sites associated with human rickettsioses in costa rica.the zoonotic transmission cycles of rickettsia rickettsii and other spotted fever group (sfg) rickettsiae in latin america have usually been associated with rural or sylvatic environments, although domestic dogs can be implicated in more populated settings. in this study, exposure of dogs to sfg rickettsiae in the greater metropolitan area of costa rica was investigated. dogs from sites associated with human cases and from dog shelters were evaluated by indirect immunofluorescence assay (ifa) us ...201626995323
rickettsia rickettsii and rickettsia felis infection in rhipicephalus sanguineus ticks and ctenocephalides felis fleas co-existing in a small city in yucatan, mexico. 201526611982
rickettsia felis in rhipicephalus sanguineus from two distant chilean cities.rickettsia felis is an emerging agent considered a human threat; although its natural reservoir and agent of transmission is the cat flea, it has been also found in other vectors. r. felis has been identified in chile in cat fleas and in one specimen of rhipicephalus sanguineus collected in the metropolitan region. the objective of this study was to detect the presence of rickettsia spp. in r. sanguineus from dogs of two different and distant geographical areas in chile.201323659352
prevalence and infection intensity of rickettsia massiliae in rhipicephalus sanguineus sensu lato ticks from mendoza, argentina.rickettsia massiliae belongs to the spotted fever group and in the new world is commonly associated with the brown dog tick, rhipicephalus sanguineus. herein we investigate the presence of r. massiliae in rh. sanguineus sensu lato ticks in a location near the andean foothills (mendoza, argentina), to provide a prevalence estimate and to assess the infection intensity of this pathogen. rickettsia massiliae infection was found in 5.1% of the rh. sanguineus s.l ticks analyzed, all with high infecti ...201627394145
isolation of rickettsia massiliae from rhipicephalus sanguineus ticks, buenos aires (argentina).rickettsia massiliae , a member of the spotted fever group of rickettsia, was first isolated from a rhipicephalus turanicus tick in france. in the new world, it has been detected in rhipicephalus sanguineus ticks from different geographical locations in argentina and the united states, but it has only been isolated in arizona. the aim of this study was the isolation and genetic characterization of r. massiliae from r. sanguineus ticks collected from dogs in buenos aires city, argentina. in total ...201526301512
quantitative study of rickettsia massiliae in rhipicephalus sanguineus organs.rickettsia massiliae, belonging to the spotted fever group of rickettsia, is a human pathogen causing a similar course of disease to that caused by r. conorii, the originally recognized etiologic agent of mediterranean spotted fever. in view of this similarity, we performed an ultrastructural study of r. massiliae in organs of rhipicephalus sanguineus ticks, in order to advance knowledge of the complex dynamics at the tick-pathogen interface in rickettsioses. adult r. massiliae-infected rh. sang ...201425108779
molecular detection of rickettsia species within ticks (acari: ixodidae) collected from arkansas united states.rocky mountain spotted fever (rmsf), caused by the etiological agent rickettsia rickettsii, is the most severe and frequently reported rickettsial illness in the united states, and is commonly diagnosed throughout the southeast. with the discoveries of rickettsia parkeri and other spotted fever group rickettsiae (sfgr) in ticks, it remains inconclusive if the cases reported as rmsf are truly caused by r. rickettsii or other sfgr. arkansas reports one of the highest incidence rates of rmsf in the ...201526334827
rickettsia parkeri infecting free-living amblyomma triste ticks in the brazilian pantanal.the present study evaluated the infection of rickettsiae in 151 rhipicephalus sanguineus, 59 amblyomma ovale, 166 amblyomma triste, one amblyomma dissimile and four amblyomma dubitatum ticks collected in the municipality of poconé, state of mato grosso, within the pantanal biome of brazil. ticks were individually processed by the hemolymph test with gimenez staining, isolation of rickettsia in vero cell culture by the shell vial technique, and polymerase chain reaction (pcr) targeting the citrat ...201525650348
epidemiology of rickettsia sp. strain atlantic rainforest in a spotted fever-endemic area of southern brazil.the present study was performed in vila itoupava, an area of the state of santa catarina, southern brazil, in which a tick-borne spotted fever illness has been endemic since 2003. notably, both the etiological agent and the vector of these spotted fever cases remain unknown. during january 2011, humans, domestic dogs, and their ticks were sampled in households that are typically surrounded by highly preserved atlantic rainforest fragments. ticks collected from dogs were amblyomma ovale (34% prev ...201425108786
isolation and maintenance of rickettsia raoultii in a rhipicephalus sanguineus tick cell line.rickettsia raoultii, a member of the spotted fever group rickettsiae, has been implicated in cases of debonel/tibola/senlat, and has been detected in dermacentor spp. and rhipicephalus pumilio ticks by pcr. r. raoultii has been isolated in mammalian and tick cell lines. this study aimed to isolate r. raoultii from spanish dermacentor marginatus in tick cell lines. a single adult d. marginatus collected from vegetation in la rioja (northen spain) in october 2012 was surface-sterilised, triturated ...201626428859
rocky mountain spotted fever in mexico: past, present, and future.rocky mountain spotted fever, a tick-borne zoonosis caused by rickettsia rickettsii, is among the most lethal of all infectious diseases in the americas. in mexico, the disease was first described during the early 1940s by scientists who carefully documented specific environmental determinants responsible for devastating outbreaks in several communities in the states of sinaloa, sonora, durango, and coahuila. these investigators also described the pivotal roles of domesticated dogs and rhipiceph ...201728365226
notes from the field: community-based prevention of rocky mountain spotted fever - sonora, mexico, 2016.rocky mountain spotted fever (rmsf), a life-threatening tickborne zoonosis caused by rickettsia rickettsii, is a reemerging disease in mexico (1,2). r. rickettsii is an intracellular bacterium that infects vascular endothelium and can cause multisystem organ failure and death in the absence of timely administration of a tetracycline-class antibiotic, typically doxycycline. epidemic rmsf, as described in parts of arizona and mexico, is associated with massive local infestations of the brown dog t ...201627880753
distribution of spotted fever group rickettsiae in hard ticks (ixodida: ixodidae) from panamanian urban and rural environments (2007-2013).tick-borne rickettsiosis is an important emerging disease in panama; to date, there have been 12 confirmed cases, including eight fatalities. to evaluate the distribution of rickettsiae in panamanian ticks, we collected questing and on-host ticks in urban and rural towns in elevations varying between 0 and 2300 m. a total of 63 sites (13 urban and 50 rural towns) were used to develop models of spatial distributions. we found the following tick species: rhipicephalus sanguineus s.l. (present in 5 ...201627068930
ixodid fauna and zoonotic agents in ticks from dogs: first report of rickettsia rickettsii in rhipicephalus sanguineus in the state of mato grosso do sul, mid-western brazil.ticks from 148 dogs from the urban area of the municipality of campo grande, state of mato grosso do sul, brazil, were collected, classified and analyzed using polymerase chain reaction (pcr) for the identification of rickettsia spp., trypanosoma cruzi and leishmania spp. a total of 2015 ticks were collected. the species rhipicephalus sanguineus (98.9 %) and amblyomma cajennense (1.1 %) were identified. molecular analysis revealed that no tick samples were infected by t. cruzi. regarding leishma ...201323229491
epidemiology of brazilian spotted fever in the atlantic forest, state of são paulo, brazil.the tick-borne bacterium rickettsia rickettsii is the aetiological agent of brazilian spotted fever (bsf). the present study evaluated tick infestations on wild and domestic animals, and the rickettsial infection in these animals and their ticks in 7 forest areas adjacent to human communities in the são paulo metropolitan area (spma). the results were compared to ecological traits of each sampled area. two main tick species, amblyomma aureolatum and rhipicephalus sanguineus, were collected from ...201222716923
detection and genetic characterization of tick-borne encephalitis virus (tbev) derived from ticks removed from red foxes (vulpes vulpes) and isolated from spleen samples of red deer (cervus elaphus) in croatia.tick-borne encephalitis (tbe) is a growing public health concern in central and northern european countries. even though tbe is a notifiable disease in croatia, there is a significant lack of information in regard to vector tick identification, distribution as well as tbe virus prevalence in ticks or animals. the aim of our study was to identify and to investigate the viral prevalence of tbe virus in ticks removed from red fox (vulpes vulpes) carcasses hunted in endemic areas in northern croatia ...201424035586
occurrence of ectoparasitic arthropods associated with rodents in hail region northern saudi arabia.ectoparasitic arthropods are a diverse element of the saudi fauna. due to this, a survey of ectoparasites associated with rodents was conducted as a preliminary study in five districts of hail region of northern saudi arabia for the first time. ectoparasites extracted from 750 rodents were sampled and identified by recording their frequency of appearance. results revealed that 1,287 ectoparasites infested 316 of the captured rodent hosts. these ectoparasites parasitized on four species of rodent ...201424838257
the novel isoxazoline ectoparasiticide fluralaner: selective inhibition of arthropod γ-aminobutyric acid- and l-glutamate-gated chloride channels and insecticidal/acaricidal activity.isoxazolines are a novel class of parasiticides that are potent inhibitors of γ-aminobutyric acid (gaba)-gated chloride channels (gabacls) and l-glutamate-gated chloride channels (glucls). in this study, the effects of the isoxazoline drug fluralaner on insect and acarid gabacl (rdl) and glucl and its parasiticidal potency were investigated. we report the identification and cdna cloning of rhipicephalus (r.) microplus rdl and glucl genes, and their functional expression in xenopus laevis oocytes ...201424365472
efficacy of chemical repellents against otobius megnini (acari: argasidae) and three species of ixodid ticks.otobius megnini (dugès), often referred to as the ear tick or spinose ear tick, is a one-host tick native to the southwestern united states and mexico. infestations of this species can cause severe irritation and may affect breeding behavior in the host. although chemical repellents are commonly used as an alternative approach to conventional arthropod vector control, information on repellency against o. megnini is lacking. this study compared repellency of ammonia, bioud(®), 98.25% deet, garlic ...201424659519
ectoparasites of free-roaming domestic cats in the central united states.free-roaming domestic cat (felis catus) populations serve as a valuable resource for studying ectoparasite prevalence. while they share a similar environment as owned cats, free-roaming cats do not receive routine veterinary care or ectoparasiticide application, giving insight into parasite risks for owned animals. we examined up to 673 infested cats presented to a trap-neuter-return (tnr) clinic in the central united states. ectoparasite prevalences on cats were as follows: fleas (71.6%), ticks ...201627692321
manual for maintenance of multi-host ixodid ticks in the laboratory.use of laboratory animals as hosts for blood-sucking arthropods remains a time-proven and the most efficient method for establishment and propagation of slowly feeding ixodid ticks, despite introduction of techniques involving artificial feeding on either animal skins or synthetic membranes. new zealand white rabbits are usually the most accessible and most suitable hosts routinely used for establishment and maintenance of a large variety of multi-host tick species. here we describe standard pro ...201627651325
new records of ticks (acari: ixodidae) from dogs, cats, humans, and some wild vertebrates in alaska: invasion potential.during 2010-2016, tick specimens were solicited from veterinarians, biologists, and members of the public in alaska. eight species of ticks were recorded from domestic dogs. some ticks were collected from dogs with recent travel histories to other countries or other u.s. states, which appears to explain records of ticks not native to alaska such as amblyomma americanum (l.) (lone star tick), ixodes scapularis (say) (blacklegged tick), and ixodes ricinus (l.). however, we recorded dermacentor var ...201627524823
repellency to ticks (acari: ixodidae) of extracts of nigella sativa (ranunculaceae) and the anti-inflammatory dogsbestfriend™.motivated by observations that the canine anti-inflammatory cream dogsbestfriend™ (dbf) appeared to deter flies, mosquitoes, and ticks from treated animals, repellent efficacy bioassays using four species of ticks were conducted with three extracts of nigella sativa l. (ranunculaceae), a constituent of dbf. the dbf cream was tested against nymphs of lone star tick, amblyomma americanum (l.). in vertical filter paper assays, the three extracts applied at 0.413 mg extract/cm(2) filter paper repell ...201627394440
determination of the effective dose of a novel oral formulation of sarolaner (simparica™) for the treatment and month-long control of fleas and ticks on dogs.three laboratory studies were conducted to determine the appropriate dose of sarolaner, a novel isoxazoline, for the treatment and month-long control of infestations of fleas and ticks on dogs. in the first study, dogs were treated orally with sarolaner suspension formulations at 1.25, 2.5 or 5.0mg/kg, and infested with dermacentor reticulatus, rhipicephalus sanguineus ticks and with ctenocephalides felis felis (cat flea) prior to treatment and then weekly for up to 8 weeks. fleas and ticks were ...201626948830
efficacy of a novel oral formulation of sarolaner (simparica™) against five common tick species infesting dogs in the united states.the efficacy of a single oral treatment with sarolaner (simparica™, zoetis), a novel isoxazoline compound, was evaluated against five tick species known to infest dogs in the united states. a total of 10 laboratory studies, two against each species, were conducted using adult purpose-bred mongrels or beagle dogs. in each study, 16 dogs were randomly allocated to one of two treatment groups based on pre-treatment host-suitability tick counts. dogs were infested with approximately 50 unfed adult a ...201626935819
ticks associated with domestic dogs and cats in florida, usa.voluntary collections of ticks from domestic dogs and cats by veterinary practitioners across florida, usa, were conducted over a 10 month period. of the 1337 ticks submitted, five species of ixodid ticks were identified and included rhipicephalus sanguineus, amblyomma americanum, a. maculatum, dermacentor variabilis, and ixodes scapularis. most ticks were collected from dogs (98.4%) with the most predominant species being r. sanguineus (94.3%). of the ticks collected from cats (1.6%), a. americ ...201626888081
the putative role of rhipicephalus microplus salivary serpins in the tick-host relationship.inflammation and hemostasis are part of the host's first line of defense to tick feeding. these systems are in part serine protease mediated and are tightly controlled by their endogenous inhibitors, in the serpin superfamily (serine protease inhibitors). from this perspective ticks are thought to use serpins to evade host defenses during feeding. the cattle tick rhipicephalus microplus encodes at least 24 serpins, of which rms-3, rms-6, and rms-17 were previously identified in saliva of this ti ...201626844868
occurrence and county-level distribution of ticks (acari: ixodoidea) in nebraska using passive surveillance.a 100 yr (1911-2011) examination of tick submissions was compiled from the u.s. national tick collection and three state databases to determine tick species occurrence in nebraska sixteen tick species were identified including amblyomma americanum (l.), dermacentor variabilis (say), dermacentor albipictus (packard), dermacentor andersoni stiles, haemaphysalis leporispalustris (packard), rhipicephalus sanguineus (latreille), and otobius megrini (dughs). amblyomma maculatum koch and ixodes scapula ...201424724283
activity of the plant-based repellent, tt-4302 against the ticks amblyomma americanum, dermacentor variabilis, ixodes scapularis and rhipicephalus sanguineus (acari: ixodidae).the plant-based repellent tt-4302 (5 % geraniol) was compared to deet (15 %) in laboratory two-choice bioassays against the ticks amblyomma americanum, dermacentor variabilis, ixodes scapularis, and rhipicephalus sanguineus. at 2.5 and 3.5 h after treatment of filter paper with tt-4302, 100 % repellency was observed for all species at both time points with the exception of i. scapularis at the 3.5 h evaluation where repellency was 95.8 %. deet was 100 % repellent at both time points for d. varia ...201423907554
ticks infesting humans in northern misiones, argentina.this work presents records of ticks infesting humans in northern misiones province, argentina. also, notes on potential transmission of tick-borne pathogens are included. a total of 282 ticks attached to researchers were collected and identified by their morphological characters. eight tick species were found: amblyomma brasiliense, amblyomma coelebs, amblyomma dubitatum, amblyomma incisum, amblyomma ovale, haemaphysalis juxtakochi, rhipicephalus sanguineus and rhipicephalus microplus. some of t ...201627135846
tick repellents and acaricides of botanical origin: a green roadmap to control tick-borne diseases?arthropods are dangerous vectors of agents of deadly diseases, which may hit as epidemics or pandemics in the increasing world population of humans and animals. among them, ticks transmit more pathogen species than any other group of blood-feeding arthropods worldwide. thus, the effective and eco-friendly control of tick vectors in a constantly changing environment is a crucial challenge. a number of novel routes have been attempted to prevent and control tick-borne diseases, including the devel ...201627146901
impact of humidity on clustered tick eggs.eclosion of larvae from clustered tick eggs (1, 5, 10, 15, 20, 25, 50, and ±1,000 eggs) of rhipicephalus sanguineus (rs), rhipicephalus microplus (rm), amblyomma cajennense (ac), and dermacentor nitens (dn) was investigated at 43, 75, and > 98% relative humidity (rh) and 25 °c. susceptibility of eggs to dehydration varied among tick species (rs < rm < ac and dn) and the number of clustered eggs but even singularized eggs of all tested species matured, and larvae eclosed at rh > 98%. a minimum of ...201425199556
acaricidal activity of essential oil from lippia sidoides on unengorged larvae and nymphs of rhipicephalus sanguineus (acari: ixodidae) and amblyomma cajennense (acari: ixodidae).the aims of this work were to identify the compounds and to investigate the acaricidal activity of the essential oil of lippia sidoides for unengorged larvae and nymphs of rhipicephalus sanguineus and amblyomma cajennense. the oil was analyzed by gas chromatography and gas chromatography/mass spectrometry. in total, 22 compounds comprising 98.5% of the total peak area were identified. the major constituent of the essential oil was thymol (69.9%). the acaricidal activity against larvae and nymphs ...201424333789
investigation of activity of monoterpenes and phenylpropanoids against immature stages of amblyomma cajennense and rhipicephalus sanguineus (acari: ixodidae).the objective of this study was to assess the acaricidal activity of carvacrol, thymol, eugenol, and (e)-cinnamaldehyde on unengorged larvae and nymphs of amblyomma cajennense and rhipicephalus sanguineus, using the modified larval packet test. carvacrol, eugenol, and (e)-cinnamaldehyde were tested at concentrations of 2.5, 5.0, 10.0, 15.0, and 20.0 μl/ml, while thymol was tested at concentrations of 2.5, 5.0, 10.0, 15.0, and 20.0 mg/ml, in all cases with 10 repetitions per treatment. for the a. ...201323842886
a survey of ectoparasites infesting urban and rural dogs of maranhão state, brazil.this study evaluated for the first time, ectoparasite infestations on dogs from urban and rural areas of the continental land of the state of maranhão, northeastern brazil. in total, 622 dogs were examined for ectoparasite infestations. overall, 392 (63.0%) were infested with ectoparasites, 154 (51.3%) of 300 urban dogs and 238 (73.9%) of 322 rural dogs. five species of ectoparasites were found, three ticks [rhipicephalus sanguineus (latreille), amblyomma ovale koch, and amblyomma cajennense (f. ...201323802466
ticks on birds in a savanna (cerrado) reserve on the outskirts of uberlândia, minas gerais, brazil.we report tick infestations on birds, in the environment and on domestic animals in a non-forested phytophysiognomy, the savanna-like cerrado sensu stricto, in a natural reserve on the outskirts of the urban area of uberlândia, minas gerais, brazil. overall, 238 birds within 50 species, 15 families and six orders were caught. passeriformes were the most numerous, with 216 birds (90.75%), among which 22 had ticks (n = 31). within this order, the prevalence of tick infestation was 10.2%, and the a ...201323471429
chemical identification of tagetes minuta linnaeus (asteraceae) essential oil and its acaricidal effect on ticks.the control of tick species that affect animal production is vital for the economic welfare of the cattle industry. this study focused on testing the acaricidal activity of the essential oil from the leaves and stems of tagetes minuta against several brazilian tick species, including rhipicephalus (boophilus) microplus, rhipicephalus sanguineus, amblyomma cajennense and argas miniatus. the chemical composition of the essential oil was determined by chromatography and spectroscopy analyses, which ...201323295821
[detection of rickettsia in ectoparasites of wild and domestic mammals from the cerro chucanti private reserve and from neighboring towns, panamá, 2007-2010].ectoparasites are the main vectors of rickettsiosis. in panama, however, limited data are available concerning the arthropod species that serve as vectors or reservoirs.201223242292
ticks (ixodida) on humans from central panama, panama (2010-2011).from january 2010 to december 2011, a total of 138 cases of ticks feeding on humans were reported from 11 locations in central panama. five of these locations were situated in forest environments, three in rural landscapes and three in urban areas. the ticks were submitted to the gorgas memorial institute, where nine species were identified among 65 specimens: amblyomma cajennense s.l., a. dissimile, a. naponense, a. oblongoguttatum, a. ovale, a. sabanerae, a. tapirellum, haemaphysalis juxtakoch ...201222544074
altitudinal and seasonal differences of tick communities in dogs from pastoralist tribes of northern kenya.studies regarding the distribution and ecology of ticks in dogs from eastern africa are scarce. our research was based on a long-term screening of ticks parasitising the domestic dogs living with indigenous people around lake turkana, mt. kulal and mt. nyiru areas, northern kenya. a total of 9977 ticks were collected from 1464 dogs of all ages and both sexes. identification was performed using morphological keys and data were analyzed using the repeated measures anova, post-hoc scheffe test and ...201526343303
an update on the ecological distribution of the ixodidae ticks in zimbabwe.in total 7657 ticks were collected from 121 dip tanks in 12 districts representative of zimbabwe's five ecological regions between september 2013 and may 2014. based on morphological traits four genera and 13 species of ticks were identified. amblyomma hebraeum (60.3 %), rhipicephalus microplus (58.7 %), rhipicephalus decoloratus (47.1 %), rhipicephalus appendiculatus (56.2 %), rhipicephalus evertsi evertsi (67.8 %), rhipicephalus (near) punctatus (13.2 %), hyalomma truncatum (38 %) and hyalomma ...201525721256
francisella-like endosymbiont in a tick collected from a chicken in southern thailand.francisella is a genus of bacterial pathogens potentially lethal to humans. we report here for the first time a novel francisella-like endosymbiont discovered in a hard-tick (rhipicephalus sanguineus s.l.) obtained from a chicken (gallus domesticus) in thailand. the phylogenetic results indicate the 16s rdna sequences of this francisella bacterium form a unique clade with the francisella-like endosymbiont of the tick species, amblyomma varanense and amblyomma helvolum, that have previously been ...201627244963
a biological/medical review of alien tick species (acari: ixodida) accidentally transferred to poland.alien tick species of the genera ixodes, amblyomma, hyalomma and rhipicephalus are sporadically transferred to the territories of the central european countries. a biological-medical review of the following alien tick species transferred to poland is presented: ixodes eldaricus, ixodes festai, amblyomma sphenodonti, amblyomma exornatumn, amblyomma flavomaculatum, amblyomma latum, amblyomma nuttalli, amblyomma quadricavum, amblyomma transversale, amblyomma varanense, hyalomma aegyptium, hyalomma ...201424930246
ectoparasites of dogs in home environments on the caribbean slope of costa rica.reports on ectoparasites on dogs in central america are scarce. the aim of this study was to identify flea, louse and tick species infesting dogs in home environments on the caribbean slope of costa rica, and determine their frequency and coexistence. ectoparasites were collected from dogs in 83 rural homes at five study sites. specimens were identified and separated according to species. fleas were the most common ectoparasite (g = 22,217, df = 8, p = 0.004). ctenocephalides felis and pulex sim ...201322832763
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